SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013012054.1 NCBI__GCF_000025725.1:WP_013012054.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

O14289 3-isopropylmalate dehydratase; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase; EC 4.2.1.33 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
36% identity, 86% coverage: 5:176/201 of query aligns to 539:709/758 of O14289

query
sites
O14289

K

K

I

F

E

T

K

V

V

V

T

E

G

G

N

I

A

A

V

A

P

P

V

L

P

P

G

M

D

A

D

N

I

V

D

D

T

T

D

D

R

K

I

I

P

P

A

K

R

Q

F

F

L

L

K

K

C

T

V

I

T

K

F

R

D

T

A

G

L

L

G

G

E

Q

Q

F

L

A

F

F

Y

Y

D

E

V

I

R

R

F

Y

D

D

E

A

D

D

G

G

K

K

P

E

L

I

-

P

N

D

F

F

P

V

I

L

D

N

R

R

P

E

Q

P

Y

Y

K

R

G

H

A

A

N

T

I

V

I

L

V

V

S

A

G

H

N

D

N

N

F

F

G

G

C

C

G

G

S

S

R

R

E

E

H

H

A

A

P

P

Q

W

S

A

I

L

K

N

K

D

A

F

G

G

F

I

D

R

A

V

I

I

A

A

G

P

S

S

Y

F

A

A

E

D

I

I

F

F

G

N

N

N

C

C

T

F

T

K

L

N

G

G

I

M

V

L

C

P

A

I

E

P

A

T

S

P

D

I

D

E

I

Q

R

V

G

N

E

D

L

M

M

M

R

K

E

A

I

A

E

E

A

N

N

Q

P

-

S

-

E

V

K

K

M

F

E

S

L

V

D

D

V

L

A

V

E

N

S

Q

S

T

V

I

S

T

F

Y

N

G

G

D

K

K

S

Q

Y

V

K

K

V

F

N

D

I

V

K

E

P

P

N

F

A

R

Q

K

A

H

A

C

F

L

L

V

A

N

G

G

T

L

Y

D

D

D

Sites not aligning to the query:

486 modified: Phosphoserine
488 modified: Phosphoserine

P9WK95 3-isopropylmalate dehydratase small subunit; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase; EC 4.2.1.33 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
29% identity, 94% coverage: 10:197/201 of query aligns to 8:189/198 of P9WK95

query
sites
P9WK95

T

S

G

G

N

I

A

G

V

V

P

P

V

L

P

R

G

R

D

S

D

N

I

V

D

D

T

T

D

D

R

Q

I

I

P

P

A

A

R

V

F

F

L

L

K

K

C

R

V

V

T

T

F

R

D

T

A

G

L

F

G

E

E

D

Q

G

L

L

F

F

Y

A

D

G

V

W

R

R

F

S

D

D

E

P

D

-

G

-

K

-

P

-

L

-

N

A

F

F

P

V

I

L

D

N

R

L

P

S

Q

P

Y

F

K

D

G

R

A

G

N

S

I

V

I

L

V

V

S

A

G

G

N

P

N

D

F

F

G

G

C

T

G

G

S

S

R

R

E

E

H

H

A

A

P

V

Q

W

S

A

I

L

K

M

K

D

A

Y

G

G

F

F

D

R

A

V

I

V

I

I

A

S

G

S

S

R

Y

F

A

G

E

D

I

I

F

F

F

R

G

G

N

N

C

A

T

G

T

K

L

A

G

G

I

L

V

L

C

A

A

A

E

E

A

V

S

A

D

Q

D

D

I

D

R

V

G

E

E

L

L

L

M

W

R

K

E

L

I

I

E

E

A

Q

N

S

P

P

S

G

E

L

K

E

M

I

E

T

L

A

D

N

V

L

A

Q

E

D

S

R

S

I

V

I

S

T

F

A

N

A

G

T

K

V

S

V

Y

L

K

P

V

F

N
x
K

I

I

K

D

P

D

N

H

A

S

Q

A

A

W

A

R

F

L

L

L

A

E

G

G

T

-

Y

L

D

D

S

D

L

I

A

A

E

L

L

T

L

L

N

R

G

K

M

L

K

D

S

E

V

I

K

E

K

A

L

F

E

E

S

G

E

A

L

C

K

A

Y

Y

K154 (≠ N161) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

Q58667 Methanogen homoaconitase small subunit; HACN; Homoaconitate hydratase; EC 4.2.1.114 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
33% identity, 88% coverage: 18:194/201 of query aligns to 11:165/170 of Q58667

query
sites
Q58667

G

G

D

D

I

V

D

D

T

T

D

D

R

A

I

I

P

P

A

G

R

P

F

Y

L

L

K
x
R

C
x
T

V

T

T

D

F

P

D

Y

A

E

L

L

G

A

E

S

Q

H

L

C

F

M

Y

A

D

G

V

I

R

-

F

-

D

D

E

E

D

-

G

-

K

-

P

-

L

-

N

N

F

F

P

P

I

-

D

-

R

-

P

K

Q

K

Y

V

K

K

G

E

A

G

N

D

I

V

I

I

V

V

S

A

G

G

N

E

N

N

F

F

G

G

C

C

G

G

S

S

R

R

E

E

H

Q

A

A

P

V

Q

I

S

A

I

I

K

K

K

Y

A

C

G

G

F

I

D

K

A

A

I

V

I

I

A

A

G

K

S

S

Y

F

A

A

E

R

I

I

F

F

F

Y

G

R

N

N

C

A

T

I

T

N

L

V

G

G

I

L

V

I

C

P

A

I

E

I

A

A

S

N

D

T

D

D

I

-

R

-

G

-

E

-

L

-

M

-

R

-

E

E

I

I

E

K

A

D

N

G

P

D

S

I

E

V

K

E

M

I

E

D

L

L

D

D

V

K

A

E

E

E

S

I

S

V

V

I

S

T

F

N

N

K

G

N

K

K

S

T

Y

I

K

K

V

C

N

E

I

T

K

P

P

K

N

G

A

L

Q

E

A

R

A

E

F

I

L

L

A

A

G

A

T

-

Y

-

D

-

S

-

L

-

A

G

E

G

L

L

L

V

N

N

G

Y

M

L

K

K

S

K

V

R

K

K

K

L

L

I

E

Q

S

S

E

K

R26 (≠ K33) mutation R->K,V: Allows the recognition of substrates of IPMI enzymes since it becomes able to efficiently catalyze citraconate hydration and 3-isopropylmalate dehydration. Largely decreases substrate affinity for cis-homo(1-3)-aconitate.
T27 (≠ C34) mutation to A: Largely decreases substrate affinity for cis-homo(1-3)-aconitate while slightly increases activity on these substrates, and also decreases substrate affinity for maleate. Gains the ability to hydrate citraconate, an IPMI substrate.

2pkpA Crystal structure of 3-isopropylmalate dehydratase (leud)from methhanocaldococcus jannaschii dsm2661 (mj1271) (see paper)
33% identity, 88% coverage: 18:194/201 of query aligns to 11:165/167 of 2pkpA

query
sites
2pkpA

G

G

D

D

D
|
D

I

V

D

D

T

T

D

D

R

A

I

I

P

P

A

G

R

P

F

Y

L

L

K

R

C

T

V

T

T

D

F

P

D

Y

A

E

L

L

G

A

E

S

Q

H

L

C

F

M

Y

A

D

G

V

I

R

-

F

-

D

D

E

E

D

-

G

-

K

-

P

-

L

-

N

N

F

F

P

P

I

-

D

-

R

-

P

K

Q

K

Y

V

K

K

G

E

A

G

N

D

I

V

I

I

V

V

S

A

G

G

N

E

N

N

F

F

G

G

C
|
C

G

G

S

S

R

R

E

E

H

Q

A

A

P

V

Q

I

S

A

I

I

K

K

K

Y

A

C

G

G

F

I

D

K

A

A

I

V

I

I

A

A

G

K

S

S

Y

F

A

A

E

R

I

I

F

F

F

Y

G

R

N

N

C

A

T

I

T

N

L

V

G

G

I

L

V

I

C

P

A

I

E

I

A

A

S

N

D

T

D

D

I

-

R

-

G

-

E

-

L

-

M

-

R

-

E

E

I

I

E

K

A

D

N

G

P

D

S

I

E

V

K

E

M

I

E

D

L

L

D

D

V

K

A

E

E

E

S

I

S

V

V

I

S

T

F

N

N

K

G

N

K

K

S

T

Y

I

K

K

V

C

N

E

I

T

K

P

P

K

N

G

A

L

Q

E

A

R

A

E

F

I

L

L

A

A

G

A

T

-

Y

-

D

-

S

-

L

-

A

G

E

G

L

L

L

V

N

N

G

Y

M

L

K

K

S

K

V

R

K

K

K

L

L

I

E

Q

S

S

E

K

binding zinc ion: D13 (= D20), C63 (= C80)

Query Sequence

>WP_013012054.1 NCBI__GCF_000025725.1:WP_013012054.1
MALGKIEKVTGNAVPVPGDDIDTDRIIPARFLKCVTFDALGEQLFYDVRFDEDGKPLNFP
IDRPQYKGANIIVSGNNFGCGSSREHAPQSIKKAGFDAIIAGSYAEIFFGNCTTLGIVCA
EASDDIRGELMREIEANPSEKMELDVAESSVSFNGKSYKVNIKPNAQAAFLAGTYDSLAE
LLNGMKSVKKLESELKYSFSA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory