SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013075572.1 NCBI__GCF_000092905.1:WP_013075572.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9X1K9 4-hydroxy-tetrahydrodipicolinate synthase; HTPA synthase; EC 4.3.3.7 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
39% identity, 95% coverage: 8:298/305 of query aligns to 2:294/294 of Q9X1K9

query
sites
Q9X1K9

L

F

K

R

G

G

S

V

I

G

V

T

P

A

L

I

V

V

T

T

P

P

F

F

D

-

E

K

E

N

G

G

R

E

F

L

D

D

E

L

K

E

T

S

F

Y

Q

E

R

R

L

L

I

V

D

R

F

Y

Q

Q

I

L

E

E

S

N

G

G

S

V

H

N

G

A

I

L

S

I

C

V

T

L

G

G

T

T

G

G

E

E

P

S

S

P

S

T

L

V

S

N

L

E

E

D

E

E

R

R

E

E

Y

K

V

L

I

V

E

S

T

R

A

T

I

L

R

E

H

I

V

V

G

D

G

G

R

K

V

I

P

P

V

V

V

I

P

V

G

G

T

A

G

G

T

T

N

N

N

S

Y

T

E

E

E

K

T

T

L

L

R

K

L

L

T

V

R

K

H

Q

A

A

Q

E

R

K

L

L

G

G

A

A

D

N

A

G

A

V

L

L

V

V

I

V

V

T

P

P

Y

Y

N

N

R

K

P

P

S

T

Q

Q

E

E

G

G

L

L

Y

Y

R

Q

Y

H

F

Y

R

K

A

Y

L

I

A

S

D

E

E

R

V

T

D

D

I

L

P

G

I

I

I

V

Y

Y

N

N

I

V

P

P

G

G

R

R

T

T

A

G

T

V

N

N

M

V

E

L

P

P

S

E

T

T

V

A

A

A

R

R

L

I

R

A

E

D

S

L

K

K

N

N

I

V

G

G

I

I

K

K

E

E

S

A

N

N

K

P

N
x
D

F
x
I

E
x
D

Q

Q

V

I

S

D

W

R

V

T

L

V

H

S

L

L

N

T

G

K

R

Q

-

A

-

R

-

S

D

D

F

F

L

M

V

V

Y

W

S

S

G

G

I

N

E

D

T

D

L

R

C

T

Y

F

P

Y

M

L

L

L

C

C

L

A

G

G

A

D

G

G

H

V

V

I

S

S

A

V

T

V

A

S

N

N

L

V

L

A

P

P

R

K

E

Q

V

M

A

V

R

E

L

L

Y

C

D

A

L

E

T

Y

A

F

A

S

G

G

R

N

W

L

E

E

E

K

A

S

R

R

D

E

L

V

H

H

Y

R

Q

K

L

L

L

R

E

P

L

L

N

M

D

K

V

A

L

L

F

F

V

V

E

E

T

T

N

N

P

P

G

I

P

P

L

V

K

K

Y

A

A

A

M

L

G

N

L

L

A

M

G

G

M

F

I

I

H

E

P

N

R

E

L

L

R

R

P

L

P

P

L

L

C

V

E

P

P

A

S

S

P

E

E

K

N

T

R

V

E

E

K

L

I

L

R

R

T

N

V

V

L

L

A

K

K

E

Y

S

G

G

L

L

DID 166:168 (≠ NFE 173:175) mutation to AAA: Exists as a monomer in solution. Decreased activity and substrate affinity. Reduced thermal stability.

1o5kA Crystal structure of dihydrodipicolinate synthase (tm1521) from thermotoga maritima at 1.80 a resolution
39% identity, 95% coverage: 8:298/305 of query aligns to 3:295/295 of 1o5kA

query
sites
1o5kA

L

F

K

R

G

G

S

V

I

G

V

T

P

A

L

I

V

V

T

T

P

P

F

F

D

-

E

K

E

N

G

G

R

E

F

L

D

D

E

L

K

E

T

S

F

Y

Q

E

R

R

L

L

I

V

D

R

F

Y

Q

Q

I

L

E

E

S

N

G

G

S

V

H

N

G

A

I

L

S

I

C

V

T

L

G

G

T
|
T

T

T

G

G

E

E

P

S

S

P

S

T

L

V

S

N

L

E

E

D

E

E

R

R

E

E

Y

K

V

L

I

V

E

S

T

R

A

T

I

L

R
x
E

H

I

V

V

G
x
D

G

G

R

K

V

I

P

P

V

V

V

I

P

V

G

G

T

A

G

G

T

T

N

N

N

S

Y

T

E

E

E

K

T

T

L

L

R

K

L

L

T

V

R

K

H

Q

A

A

Q

E

R

K

L

L

G

G

A

A

D

N

A

G

A

V

L

L

V

V

I

V

V

T

P

P

Y

Y

Y
|
Y

N

N

R

K

P

P

S

T

Q

Q

E

E

G

G

L

L

Y

Y

R

Q

Y

H

F

Y

R

K

A

Y

L

I

A

S

D

E

E

R

V

T

D

D

I

L

P

G

I

I

I

V

Y
|
Y

N

N

I

V

P

P

G

G

R
|
R

T

T

A

G

T

V

N

N

M

V

E

L

P

P

S

E

T

T

V

A

A

A

R

R

L

I

R

A

E

D

S

L

K

K

N

N

I

V

G

G

I

I

K
|
K

E

E

S

A

N

N

K

P

N

D

F

I

E

D

Q

Q

V

I

S

D

W

R

V

T

L

V

H

S

L

L

N

T

G

K

R

Q

-

A

-

R

-

S

D

D

F

F

L

M

V

V

Y

W

S

S

G

G

I

N

E

D

T

D

L

R

C

T

Y

F

P

Y

M

L

L

L

C

C

L

A

G

G

A

D

G

G

H

V

V
x
I

S

S

A

V

T

V

A

S

N

N

L

V

L

A

P

P

R

K

E

Q

V

M

A

V

R

E

L

L

Y

C

D

A

L

E

T

Y

A

F

A

S

G

G

R

N

W

L

E

E

E

K

A

S

R

R

D

E

L

V

H

H

Y

R

Q

K

L

L

L

R

E

P

L

L

N

M

D

K

V

A

L

L

F

F

V

V

E

E

T

T

N

N

P

P

G

I

P

P

L

V

K

K

Y

A

A

A

M

L

G

N

L

L

A

M

G

G

M

F

I

I

H

E

P

N

R

E

L

L

R

R

P

L

P

P

L

L

C

V

E

P

P

A

S

S

P

E

E

K

N

T

R

V

E

E

K

L

I

L

R

R

T

N

V

V

L

L

A

K

K

E

Y

S

G

G

L

L

active site: T44 (= T50), Y107 (= Y113), Y133 (= Y139), R138 (= R144), K162 (= K168), I207 (≠ V210)
binding calcium ion: E65 (≠ R71), D68 (≠ G74)

3s8hA Structure of dihydrodipicolinate synthase complexed with 3- hydroxypropanoic acid(hpa)at 2.70 a resolution
42% identity, 96% coverage: 8:299/305 of query aligns to 2:292/292 of 3s8hA

query
sites
3s8hA

L

I

K

A

G

G

S

S

I

M

V

V

P
x
A

L
|
L

V

V

T

T

P

P

F

F

D

D

E

A

E

Q

G

G

R

R

F

L

D

D

E

W

K

D

T

S

F

L

Q

A

R

K

L

L

I

V

D

D

F

F

Q

H

I

L

E

Q

S

D

G

G

S

T

H

N

G

A

I

I

S
x
V

C

A

T

V

G
|
G

T
|
T

G
|
G

E

E

P

S

S

A

S

T

L

L

S

D

L

V

E

E

R

H

E

I

Y

Q

V

V

I

V

E

R

T

R

A

V

I

V

R

D

H

Q

V

V

G

K

G

G

R

R

V

I

P

P

V

V

V

I

P

A

G

G

T

T

G

G

T

A

N

N

N

S

Y

T

E

R

E

E

T

A

L

V

R

A

L

L

T

T

R

E

H

A

A

A

Q

K

R

S

L

G

G

G

A

A

D

D

A

A

A

C

L

L

V

L

I

V

V

T

P

P

Y

Y

Y
|
Y

N

N

R

K

P

P

S

T

Q

Q

E

E

G

G

L

M

Y

Y

R

Q

Y

H

F

F

R

R

A

H

L

I

A

A

D

E

E

A

V

V

D

A

I

I

P

P

I

Q

I

I

I

L

Y
|
Y

N

N

I

V

P

P

G

G

R
|
R

T

T

A

S

T

C

N

D

M

M

E

L

P

P

S

E

T

T

V

V

A

E

R

R

L

L

R

S

R

K

E

-

S

V

K

P

N

N

I

I

G

G

I

I

K
|
K

E

E

S

A

N

T

K

G

N

D

F

L

E

Q

Q

R

V

A

S

K

W

E

V

V

L

I

H

E

L

R

N

V

G

G

R

K

D

D

F

F

L

L

V

V

Y

Y

S

S

G

G

I

D

E

D

T

A

L

T

C

A

Y

V

P

E

M

L

L

M

C

L

L

L

G

G

A

K

G

G

H

N

V
x
I

S

S

A

V

T

T

A

A

N

N

L

V

L

A

P

P

R

R

E

A

V

M

A

S

R

D

L

L

Y

C

D

A

L

A

T

A

A

M

A

R

G

G

R

D

W

A

E

A

A

A

R

R

D

A

L

I

H

N

Y

D

Q

R

L

L

L

M

E

P

L

L

N

H

D

K

V

A

L

L

F

F

V

I

E

E

T

S

N

N

P

P

G

I

P

P

L

V

K

K

Y

W

A

A

M

L

G

H

L

E

A

M

G

G

M

L

I

I

H

P

P

E

R

G

L

I

R

R

P

L

P

P

L

L

C

T

E

W

P

L

S

S

P

P

E

H

N

C

R

H

E

D

K

P

I

L

R

R

T

Q

V

A

L

M

A

R

K

Q

Y

T

G

G

L

V

L

L

A

A

active site: T44 (= T50), Y107 (= Y113), Y133 (= Y139), R138 (= R144), K161 (= K168), I203 (≠ V210)
binding 3-hydroxy-propanoic acid: A8 (≠ P14), L9 (= L15), V40 (≠ S46), G43 (= G49), T44 (= T50), T45 (= T51), G46 (= G52), K161 (= K168)

3puoA Crystal structure of dihydrodipicolinate synthase from pseudomonas aeruginosa(psdhdps)complexed with l-lysine at 2.65a resolution (see paper)
42% identity, 96% coverage: 8:299/305 of query aligns to 2:292/292 of 3puoA

query
sites
3puoA

L

I

K

A

G

G

S

S

I

M

V

V

P

A

L

L

V

V

T

T

P

P

F

F

D

D

E

A

E

Q

G

G

R

R

F

L

D

D

E

W

K

D

T

S

F

L

Q

A

R

K

L

L

I

V

D

D

F

F

Q

H

I

L

E

Q

S

D

G

G

S

T

H

N

G

A

I

I

S

V

C

A

T

V

G

G

T
|
T

T

T

G

G

E

E

P
x
S

S
x
A

S

T

L
|
L

S
x
D

L
x
V

E

E

R

H

E

I

Y

Q

V

V

I

V

E

R

T

R

A

V

I

V

R

D

H

Q

V

V

G

K

G

G

R

R

V

I

P

P

V

V

V

I

P

A

G

G

T

T

G

G

T

A

N

N

N

S

Y

T

E

R

E

E

T

A

L

V

R

A

L

L

T

T

R

E

H

A

A

A

Q

K

R

S

L

G

G

G

A

A

D

D

A

A

A

C

L

L

V

L

I

V

V

T

P

P

Y

Y

Y
|
Y

N

N

R

K

P

P

S

T

Q

Q

E

E

G

G

L

M

Y

Y

R

Q

Y

H

F

F

R

R

A

H

L

I

A

A

D

E

E

A

V

V

D

A

I

I

P

P

I

Q

I

I

I

L

Y
|
Y

N

N

I

V

P

P

G

G

R
|
R

T

T

A

S

T

C

N

D

M

M

E

L

P

P

S

E

T

T

V

V

A

E

R

R

L

L

R

S

R

K

E

-

S

V

K

P

N

N

I

I

G

G

I

I

K
|
K

E

E

S

A

N

T

K

G

N

D

F

L

E

Q

Q

R

V

A

S

K

W

E

V

V

L

I

H

E

L

R

N

V

G

G

R

K

D

D

F

F

L

L

V

V

Y

Y

S

S

G

G

I

D

E

D

T

A

L

T

C

A

Y

V

P

E

M

L

L

M

C

L

L

L

G

G

A

K

G

G

H

N

V
x
I

S

S

A

V

T

T

A

A

N

N

L

V

L

A

P

P

R

R

E

A

V

M

A

S

R

D

L

L

Y

C

D

A

L

A

T

A

A

M

A

R

G

G

R

D

W

A

E

A

A

A

R

R

D

A

L

I

H

N

Y

D

Q

R

L

L

L

M

E

P

L

L

N

H

D

K

V

A

L

L

F

F

V

I

E

E

T

S

N

N

P

P

G

I

P

P

L

V

K

K

Y

W

A

A

M

L

G

H

L

E

A

M

G

G

M

L

I

I

H

P

P

E

R

G

L
x
I

R

R

P
x
L

P

P

L
|
L

C
x
T

E

W

P

L

S

S

P

P

E

H

N

C

R

H

E

D

K

P

I

L

R

R

T

Q

V

A

L

M

A

R

K

Q

Y

T

G

G

L

V

L

L

A

A

active site: T44 (= T50), Y107 (= Y113), Y133 (= Y139), R138 (= R144), K161 (= K168), I203 (≠ V210)
binding lysine: S48 (≠ P54), A49 (≠ S55), L51 (= L57), D52 (≠ S58), V53 (≠ L59), I266 (≠ L273), L268 (≠ P275), L270 (= L277), T271 (≠ C278)

4dxvA Crystal structure of dihydrodipicolinate synthase from acinetobacter baumannii complexed with mg and cl ions at 1.80 a resolution
39% identity, 95% coverage: 8:298/305 of query aligns to 2:291/291 of 4dxvA

query
sites
4dxvA

L

I

K

Q

G

G

S

S

I

I

V

V

P

A

L

I

V

V

T

T

P

P

F

M

D

L

E

K

E

D

G

G

R

G

F

V

D

D

E

W

K

K

T

S

F

L

Q

E

R

K

L

L

I

V

D

E

F

W

Q

H

I

I

E

E

S

Q

G

G

S

T

H

N

G

S

I

I

S

V

C

A

T

V

G

G

T
|
T

T

T

G

G

E

E

P

A

S

S

S

T

L

L

S

S

L

M

E

E

R

H

E

T

Y

Q

V

V

I

I

E

K

T

E

A

I

I

I

R

R

H

V

V

A

G

N

G

K

R

R

V

I

P

P

V

I

P

A

G

G

T

T

G

G

T

A

N

N

N

S

Y

T

E

R

E

E

T

A

L

I

R

E

L

L

T

T

R

K

H

A

A

A

Q

K

R

D

L

L

G

G

A

A

D

D

A

A

L

L

V

L

I

V

V
x
T

P

P

Y

Y

Y
|
Y

N

N

R

K

P

P

S

T

Q

Q

E

E

G

G

L

L

Y

Y

R

Q

Y

H

F

Y

R

K

A

A

L

I

A

A

D

E

E

A

V

V

D

E

I

L

P

P

I

L

I

I

I

L

Y
|
Y

N
|
N

I
x
V

P
|
P

G

G

R
|
R

T
|
T

A

G

T

V

N

D

M

L

E

S

P

N

S

D

T

T

V

A

A

V

R

R

L

L

R

-

R

A

E

E

S

I

K

P

N

N

I

I

I

V

G

G

I

I

K
|
K

E

D

S

A

N

T

K

G

N

D

F

V

E

P

Q

R

V

G

S

K

W

A

V

L

L

I

H

D

-

A

L

L

N

N

G

G

R

K

D

-

F

M

L

A

V

V

Y

Y

S

S

G

G

I

D

E

D

T

E

L

T

C

A

Y

W

P

E

M

L

L

M

C

L

L

L

G

G

G

A

A

D

G

G

H

N

V
x
I

S

S

A

V

T

T

A

A

N

N

L

I

L

A

P

P

R

K

E

A

V

M

A

S

R

E

L

V

Y

C

D

A

L

V

T

A

A

I

A

A

G

K

R

D

W

E

E

Q

A

A

R

K

D

T

L

L

H

N

Y

N

Q

K

L

I

L

A

E

N

L

L

N

H

D

N

V

I

L

L

F

F

V

C

E

E

T

S

N

N

P

P

G

I

P

P

L

V

K

K

Y

W

A

A

M

L

G

H

L

E

A

M

G

G

M

L

I

I

H

D

P

T

R

G

L

I

R

R

P

L

P

P

L

L

C

T

E

P

P

L

S

A

P

E

E

Q

N

Y

R

R

E

E

K

P

I

L

R

R

T

N

V

A

L

L

A

K

K

D

Y

A

G

G

L

I

active site: T44 (= T50), Y107 (= Y113), Y133 (= Y139), R138 (= R144), K161 (= K168), I203 (≠ V210)
binding magnesium ion: T104 (≠ V110), N134 (= N140), V135 (≠ I141), P136 (= P142), T139 (= T145)

3u8gA Crystal structure of the complex of dihydrodipicolinate synthase from acinetobacter baumannii with oxalic acid at 1.80 a resolution
39% identity, 95% coverage: 8:298/305 of query aligns to 2:291/291 of 3u8gA

query
sites
3u8gA

L

I

K

Q

G

G

S

S

I

I

V

V

P
x
A

L

I

V

V

T

T

P

P

F

M

D

L

E

K

E

D

G

G

R

G

F

V

D

D

E

W

K

K

T

S

F

L

Q

E

R

K

L

L

I

V

D

E

F

W

Q

H

I

I

E

E

S

Q

G

G

S

T

H

N

G

S

I

I

S

V

C

A

T

V

G
|
G

T
|
T

G

G

E

E

P

A

S

S

S

T

L

L

S

S

L

M

E

E

R

H

E

T

Y

Q

V

V

I

I

E

K

T

E

A

I

I

I

R

R

H

V

V

A

G

N

G

K

R

R

V

I

P

P

V

I

P

A

G

G

T

T

G

G

T

A

N

N

N

S

Y

T

E

R

E

E

T

A

L

I

R

E

L

L

T

T

R

K

H

A

A

A

Q

K

R

D

L

L

G

G

A

A

D

D

A

A

L
|
L

V

L

I

V

V

T

P

P

Y

Y

Y
|
Y

N

N

R

K

P

P

S

T

Q

Q

E

E

G

G

L

L

Y

Y

R

Q

Y

H

F

Y

R

K

A

A

L

I

A

A

D

E

E

A

V

V

D

E

I

L

P

P

I

L

I

I

I

L

Y
|
Y

N

N

I

V

P

P

G

G

R
|
R

T

T

A

G

T

V

N

D

M

L

E

S

P

N

S

D

T

T

V

A

A

V

R

R

L

L

R

-

R

A

E

E

S

I

K

P

N

N

I

I

I

V

G

G

I

I

K
|
K

E

D

S

A

N

T

K

G

N

D

F

V

E

P

Q

R

V

G

S

K

W

A

V

L

L

I

H

D

-

A

L

L

N

N

G

G

R

K

D

-

F

M

L

A

V

V

Y

Y

S

S

G

G

I

D

E

D

T

E

L

T

C

A

Y

W

P

E

M

L

L

M

C

L

L

L

G

G

G

A

A

D

G

G

H

N

V
x
I

S

S

A

V

T

T

A

A

N

N

L

I

L

A

P

P

R

K

E

A

V

M

A

S

R

E

L

V

Y

C

D

A

L

V

T

A

A

I

A

A

G

K

R

D

W

E

E

Q

A

A

R

K

D

T

L

L

H

N

Y

N

Q

K

L

I

L

A

E

N

L

L

N

H

D

N

V

I

L

L

F

F

V

C

E

E

T

S

N

N

P

P

G

I

P

P

L

V

K

K

Y

W

A

A

M

L

G

H

L

E

A

M

G

G

M

L

I

I

H

D

P

T

R

G

L

I

R

R

P

L

P

P

L

L

C

T

E

P

P

L

S

A

P

E

E

Q

N

Y

R

R

E

E

K

P

I

L

R

R

T

N

V

A

L

L

A

K

K

D

Y

A

G

G

L

I

active site: T44 (= T50), Y107 (= Y113), Y133 (= Y139), R138 (= R144), K161 (= K168), I203 (≠ V210)
binding oxalate ion: A8 (≠ P14), G43 (= G49), T44 (= T50), T45 (= T51), L101 (= L107), Y133 (= Y139), K161 (= K168)

3tdfA Crystal structure of the complex of dihydrodipicolinate synthase from acinetobacter baumannii with 2-ketobutanoic acid at 1.99 a resolution
39% identity, 95% coverage: 8:298/305 of query aligns to 2:291/291 of 3tdfA

query
sites
3tdfA

L

I

K

Q

G

G

S

S

I

I

V

V

P
x
A

L

I

V

V

T

T

P

P

F

M

D

L

E

K

E

D

G

G

R

G

F

V

D

D

E

W

K

K

T

S

F

L

Q

E

R

K

L

L

I

V

D

E

F

W

Q

H

I

I

E

E

S

Q

G

G

S

T

H

N

G

S

I

I

S

V

C

A

T

V

G

G

T
|
T

G

G

E

E

P

A

S

S

S

T

L

L

S

S

L

M

E

E

R

H

E

T

Y

Q

V

V

I

I

E

K

T

E

A

I

I

I

R

R

H

V

V

A

G

N

G

K

R

R

V

I

P

P

V

I

P

A

G

G

T

T

G

G

T

A

N

N

N

S

Y

T

E

R

E

E

T

A

L

I

R

E

L

L

T

T

R

K

H

A

A

A

Q

K

R

D

L

L

G

G

A

A

D

D

A

A

L

L

V

L

I

V

V

T

P

P

Y

Y

Y
|
Y

N

N

R

K

P

P

S

T

Q

Q

E

E

G

G

L

L

Y

Y

R

Q

Y

H

F

Y

R

K

A

A

L

I

A

A

D

E

E

A

V

V

D

E

I

L

P

P

I

L

I

I

I

L

Y
|
Y

N

N

I

V

P

P

G

G

R
|
R

T

T

A

G

T

V

N

D

M

L

E

S

P

N

S

D

T

T

V

A

A

V

R

R

L

L

R

-

R

A

E

E

S

I

K

P

N

N

I

I

I

V

G

G

I

I

K
|
K

E

D

S

A

N

T

K

G

N

D

F

V

E

P

Q

R

V

G

S

K

W

A

V

L

L

I

H

D

-

A

L

L

N

N

G

G

R

K

D

-

F

M

L

A

V

V

Y

Y

S

S

G

G

I

D

E

D

T

E

L

T

C

A

Y

W

P

E

M

L

L

M

C

L

L

L

G

G

G

A

A

D

G

G

H

N

V
x
I

S

S

A

V

T

T

A

A

N

N

L

I

L

A

P

P

R

K

E

A

V

M

A

S

R

E

L

V

Y

C

D

A

L

V

T

A

A

I

A

A

G

K

R

D

W

E

E

Q

A

A

R

K

D

T

L

L

H

N

Y

N

Q

K

L

I

L

A

E

N

L

L

N

H

D

N

V

I

L

L

F

F

V

C

E

E

T

S

N

N

P

P

G

I

P

P

L

V

K

K

Y

W

A

A

M

L

G

H

L

E

A

M

G

G

M

L

I

I

H

D

P

T

R

G

L

I

R

R

P

L

P

P

L

L

C

T

E

P

P

L

S

A

P

E

E

Q

N

Y

R

R

E

E

K

P

I

L

R

R

T

N

V

A

L

L

A

K

K

D

Y

A

G

G

L

I

active site: T44 (= T50), Y107 (= Y113), Y133 (= Y139), R138 (= R144), K161 (= K168), I203 (≠ V210)
binding 2-ketobutyric acid: A8 (≠ P14), T44 (= T50), T45 (= T51), Y133 (= Y139), K161 (= K168), I203 (≠ V210)

3tceA Crystal structure of the complex of dihydrodipicolinate synthase from acinetobacter baumannii with 5-hydroxylysine at 2.6 a resolution
39% identity, 95% coverage: 8:298/305 of query aligns to 2:291/291 of 3tceA

query
sites
3tceA

L

I

K

Q

G

G

S

S

I

I

V

V

P

A

L

I

V

V

T

T

P

P

F

M

D

L

E

K

E

D

G

G

R

G

F

V

D

D

E

W

K

K

T

S

F

L

Q

E

R

K

L

L

I

V

D

E

F

W

Q

H

I

I

E

E

S

Q

G

G

S

T

H

N

G

S

I

I

S

V

C

A

T

V

G

G

T
|
T

T

T

G

G

E

E

P

A

S
|
S

S

T

L
|
L

S

S

L

M

E

E

R
x
H

E

T

Y

Q

V

V

I

I

E

K

T

E

A

I

I

I

R

R

H

V

V

A

G

N

G

K

R

R

V

I

P

P

V

I

P

A

G

G

T

T

G

G

T

A

N

N

N

S

Y

T

E

R

E

E

T

A

L

I

R

E

L

L

T

T

R

K

H

A

A

A

Q

K

R

D

L

L

G

G

A

A

D

D

A

A

L

L

V

L

I

V

V

T

P

P

Y
|
Y

N

N

R

K

P

P

S

T

Q

Q

E

E

G

G

L

L

Y

Y

R

Q

Y

H

F

Y

R

K

A

A

L

I

A

A

D

E

E

A

V

V

D

E

I

L

P

P

I

L

I

I

I

L

Y
|
Y

N

N

I

V

P

P

G

G

R
|
R

T

T

A

G

T

V

N

D

M

L

E

S

P

N

S

D

T

T

V

A

A

V

R

R

L

L

R

-

R

A

E

E

S

I

K

P

N

N

I

I

I

V

G

G

I

I

K
|
K

E

D

S

A

N

T

K

G

N

D

F

V

E

P

Q

R

V

G

S

K

W

A

V

L

L

I

H

D

-

A

L

L

N

N

G

G

R

K

D

-

F

M

L

A

V

V

Y

Y

S

S

G

G

I

D

E

D

T

E

L

T

C

A

Y

W

P

E

M

L

L

M

C

L

L

L

G

G

G

A

A

D

G

G

H

N

V
x
I

S

S

A

V

T

T

A

A

N

N

L

I

L

A

P

P

R

K

E

A

V

M

A

S

R

E

L

V

Y

C

D

A

L

V

T

A

A

I

A

A

G

K

R

D

W

E

E

Q

A

A

R

K

D

T

L

L

H

N

Y

N

Q

K

L

I

L

A

E

N

L

L

N

H

D

N

V

I

L

L

F

F

V

C

E

E

T

S

N

N

P

P

G

I

P

P

L

V

K

K

Y

W

A

A

M

L

G

H

L

E

A

M

G

G

M

L

I

I

H

D

P

T

R

G

L

I

R

R

P

L

P

P

L

L

C

T

E

P

P

L

S

A

P

E

E

Q

N

Y

R

R

E

E

K

P

I

L

R

R

T

N

V

A

L

L

A

K

K

D

Y

A

G

G

L

I

active site: T44 (= T50), Y107 (= Y113), Y133 (= Y139), R138 (= R144), K161 (= K168), I203 (≠ V210)
binding 5-hydroxylysine: S49 (= S55), L51 (= L57), H56 (≠ R62), Y106 (= Y112)

3rk8A Crystal structure of the chloride inhibited dihydrodipicolinate synthase from acinetobacter baumannii complexed with pyruvate at 1.8 a resolution
39% identity, 95% coverage: 8:298/305 of query aligns to 2:291/291 of 3rk8A

query
sites
3rk8A

L

I

K

Q

G

G

S

S

I

I

V

V

P

A

L

I

V

V

T

T

P

P

F

M

D

L

E

K

E

D

G

G

R

G

F

V

D

D

E

W

K

K

T

S

F

L

Q

E

R

K

L

L

I

V

D

E

F

W

Q

H

I

I

E

E

S

Q

G

G

S

T

H

N

G

S

I

I

S

V

C

A

T

V

G

G

T
|
T

T

T

G

G

E

E

P

A

S

S

S

T

L

L

S

S

L

M

E

E

R

H

E

T

Y

Q

V

V

I

I

E

K

T

E

A

I

I

I

R

R

H

V

V

A

G

N

G

K

R

R

V

I

P

P

V

I

P

A

G

G

T

T

G

G

T

A

N

N

N

S

Y

T

E

R

E

E

T

A

L

I

R

E

L

L

T

T

R

K

H

A

A

A

Q

K

R

D

L

L

G

G

A

A

D

D

A

A

L

L

V

L

I

V

V

T

P

P

Y

Y

Y
|
Y

N

N

R

K

P

P

S

T

Q

Q

E

E

G

G

L

L

Y

Y

R

Q

Y

H

F

Y

R

K

A

A

L

I

A

A

D

E

E

A

V

V

D

E

I

L

P

P

I

L

I

I

I

L

Y
|
Y

N

N

I

V

P

P

G

G

R
|
R

T

T

A

G

T

V

N

D

M

L

E

S

P

N

S

D

T

T

V

A

A

V

R

R

L

L

R

-

R

A

E

E

S

I

K

P

N

N

I

I

I

V

G

G

I

I

K
|
K

E

D

S

A

N

T

K

G

N

D

F

V

E

P

Q

R

V

G

S

K

W

A

V

L

L

I

H

D

-

A

L

L

N

N

G

G

R

K

D

-

F

M

L

A

V

V

Y

Y

S

S

G
|
G

I

D

E

D

T

E

L

T

C

A

Y

W

P

E

M

L

L

M

C

L

L

L

G

G

G

A

A

D

G

G

H

N

V
x
I

S

S

A

V

T

T

A

A

N

N

L

I

L

A

P

P

R

K

E

A

V

M

A

S

R

E

L

V

Y

C

D

A

L

V

T

A

A

I

A

A

G

K

R

D

W

E

E

Q

A

A

R

K

D

T

L

L

H

N

Y

N

Q

K

L

I

L

A

E

N

L

L

N

H

D

N

V

I

L

L

F
|
F

V

C

E

E

T

S

N

N

P

P

G

I

P

P

L

V

K

K

Y

W

A

A

M

L

G

H

L

E

A

M

G

G

M

L

I

I

H

D

P

T

R

G

L

I

R

R

P

L

P

P

L

L

C

T

E

P

P

L

S

A

P

E

E

Q

N

Y

R

R

E

E

K

P

I

L

R

R

T

N

V

A

L

L

A

K

K

D

Y

A

G

G

L

I

active site: T44 (= T50), Y107 (= Y113), Y133 (= Y139), R138 (= R144), K161 (= K168), I203 (≠ V210)
binding pyruvic acid: R138 (= R144), K161 (= K168), G186 (= G193), F244 (= F251)

3pueB Crystal structure of the complex of dhydrodipicolinate synthase from acinetobacter baumannii with lysine at 2.6a resolution
39% identity, 95% coverage: 8:298/305 of query aligns to 2:291/291 of 3pueB

query
sites
3pueB

L

I

K

Q

G

G

S

S

I

I

V

V

P

A

L

I

V

V

T

T

P

P

F

M

D

L

E

K

E

D

G

G

R

G

F

V

D

D

E

W

K

K

T

S

F

L

Q

E

R

K

L

L

I

V

D

E

F

W

Q

H

I

I

E

E

S

Q

G

G

S

T

H

N

G

S

I

I

S

V

C

A

T

V

G

G

T
|
T

T

T

G

G

E

E

P

A

S

S

S

T

L

L

S

S

L

M

E

E

R

H

E

T

Y

Q

V

V

I

I

E

K

T

E

A

I

I

I

R

R

H

V

V

A

G

N

G

K

R

R

V

I

P

P

V

I

P

A

G

G

T

T

G

G

T

A

N

N

N

S

Y

T

E

R

E

E

T

A

L

I

R

E

L

L

T

T

R

K

H

A

A

A

Q

K

R

D

L

L

G

G

A

A

D

D

A

A

L

L

V

L

I

V

V

T

P

P

Y

Y

Y
|
Y

N

N

R

K

P

P

S

T

Q

Q

E

E

G

G

L

L

Y

Y

R

Q

Y

H

F

Y

R

K

A

A

L

I

A

A

D

E

E

A

V

V

D

E

I

L

P

P

I

L

I

I

I

L

Y
|
Y

N

N

I

V

P

P

G

G

R
|
R

T

T

A

G

T

V

N

D

M

L

E

S

P

N

S

D

T

T

V

A

A

V

R

R

L

L

R

-

R

A

E

E

S

I

K

P

N

N

I

I

I

V

G

G

I

I

K
|
K

E

D

S

A

N

T

K

G

N

D

F

V

E

P

Q

R

V

G

S

K

W

A

V

L

L

I

H

D

-

A

L

L

N

N

G

G

R

K

D

-

F

M

L

A

V

V

Y

Y

S

S

G

G

I

D

E

D

T

E

L

T

C

A

Y

W

P

E

M

L

L

M

C

L

L

L

G

G

G

A

A

D

G

G

H

N

V
x
I

S

S

A

V

T

T

A

A

N

N

L

I

L

A

P

P

R

K

E

A

V

M

A

S

R

E

L

V

Y

C

D

A

L

V

T

A

A

I

A

A

G

K

R

D

W

E

E

Q

A

A

R

K

D

T

L

L

H

N

Y

N

Q

K

L

I

L

A

E

N

L

L

N

H

D

N

V

I

L

L

F
|
F

V

C

E

E

T

S

N
|
N

P

P

G

I

P

P

L

V

K

K

Y

W

A

A

M

L

G

H

L

E

A

M

G

G

M

L

I

I

H

D

P

T

R

G

L

I

R

R

P

L

P

P

L

L

C

T

E

P

P

L

S

A

P

E

E

Q

N

Y

R

R

E

E

K

P

I

L

R

R

T

N

V

A

L

L

A

K

K

D

Y

A

G

G

L

I

active site: T44 (= T50), Y107 (= Y113), Y133 (= Y139), R138 (= R144), K161 (= K168), I203 (≠ V210)
binding lysine: R138 (= R144), F244 (= F251), N248 (= N255)

7kg2A Dihydrodipicolinate synthase (dhdps) from c.Jejuni, h59k mutant with pyruvate bound in the active site and l-histidine bound at the allosteric site
40% identity, 94% coverage: 10:297/305 of query aligns to 6:293/296 of 7kg2A

query
sites
7kg2A

G

G

S

A

I

M

V

T

P

A

L

L

V

I

T

T

P

P

F

F

D

-

E

K

E

N

G

G

R

K

F

V

D

D

E

E

K

Q

T

S

F

Y

Q

A

R

R

L

L

I

I

D

K

F

R

Q

Q

I

I

E

E

S

N

G

G

S

I

H

D

G

A

I

V

S

V

C

P

T

V

G

G

T

T

G

G

E

E

P
x
S

S
x
A

S

T

L
|
L

S

T

L

H

E

E

R
x
K

E

R

Y

T

V

C

I

I

E

E

T

I

A

A

I

V

R

E

H

T

V

C

G

K

G

G

-

T

R

K

V

V

P

K

V

V

V

L

P

A

G

G

T

A

G

G

T

S

N
|
N

N

A

Y

T

E

H

E
|
E

T

A

L

V

R

G

L

L

T

A

R

K

H

F

A

A

Q

K

R

E

L

H

G

G

A

A

D

D

A

G

A

I

L

L

V

S

I

V

V

A

P

P

Y
|
Y

Y

Y

N

N

R

K

P

P

S

T

Q

Q

E

Q

G

G

L

L

Y

Y

R

E

Y

H

F

Y

R

K

A

A

L

I

A

A

D

Q

E

S

V

V

D

D

I

I

P

P

I

V

I

L

Y

Y

N

N

I

V

P

P

G

G

R

R

T

T

A

G

T

C

N

E

M

I

E

S

P

T

S

D

T

T

V

I

A

I

R

K

L

L

R

F

R

R

E

D

S

C

K

E

N

N

I

I

I

Y

G

G

I

V

K

K

E

E

S

A

N

S

K

G

N

N

F

I

E

D

Q

K

V

C

S

V

W

D

V

L

L

L

H

A

L

H

N

E

G

P

R

R

D

M

F

M

L

L

V

I

Y

-

S

S

G

G

I

E

E

D

T

A

L

I

C

N

Y

Y

P

P

M

I

L

L

C

S

L

N

G

G

A

K

G

G

H

V

V

I

S

S

A

V

T

T

A

S

N

N

L

L

P

P

R

D

E

M

V

I

A

S

R

A

L

L

Y

T

D

H

L

F

T

A

A

L

A

D

G

E

R

N

W

Y

E

K

E

E

A

A

R

K

D

K

L

I

H

N

Y

D

Q

E

L

L

L

Y

E

N

L

I

N

N

D

K

V

I

L

L

F

F

V

C

E

E

T

S

N

N

P

P

G

I

P

P

L

I

K

K

Y

T

A

A

M

M

G

Y

L

L

A

A

G

G

M

L

I

I

H

E

P

S

-

L

R

E

L

F

R

R

P

L

P

P

L

L

C

C

E

S

P

P

S

S

P

K

E

E

N

N

R

F

E

A

K

K

I

I

R

E

T

E

V

V

L

M

A

K

K

K

Y

Y

G

K

L

I

binding histidine: S49 (≠ P54), A50 (≠ S55), L52 (= L57), K57 (≠ R62), N82 (= N86), E86 (= E90), Y108 (= Y112)

4m19A Dihydrodipicolinate synthase from c. Jejuni with pyruvate bound to the active site and lysine bound to allosteric site (see paper)
40% identity, 94% coverage: 10:297/305 of query aligns to 6:293/296 of 4m19A

query
sites
4m19A

G

G

S

A

I

M

V

T

P

A

L

L

V

I

T

T

P

P

F

F

D

-

E

K

E

N

G

G

R

K

F

V

D

D

E

E

K

Q

T

S

F

Y

Q

A

R

R

L

L

I

I

D

K

F

R

Q

Q

I

I

E

E

S

N

G

G

S

I

H

D

G

A

I

V

S

V

C

P

T

V

G

G

T
|
T

T

T

G

G

E

E

P
x
S

S
x
A

S

T

L
|
L

S

T

L
x
H

E

E

R
x
H

E

R

Y

T

V

C

I

I

E

E

T

I

A

A

I

V

R

E

H

T

V

C

G

K

G

G

-

T

R

K

V

V

P

K

V

V

V

L

P

A

G

G

T

A

G

G

T

S

N
|
N

N

A

Y

T

E

H

E
|
E

T

A

L

V

R

G

L

L

T

A

R

K

H

F

A

A

Q

K

R

E

L

H

G

G

A

A

D

D

A

G

A

I

L

L

V

S

I

V

V

A

P

P

Y
|
Y

N

N

R

K

P

P

S

T

Q

Q

E

Q

G

G

L

L

Y

Y

R

E

Y

H

F

Y

R

K

A

A

L

I

A

A

D

Q

E

S

V

V

D

D

I

I

P

P

I

V

I

L

Y
|
Y

N

N

I

V

P

P

G

G

R
|
R

T

T

A

G

T

C

N

E

M

I

E

S

P

T

S

D

T

T

V

I

A

I

R

K

L

L

R

F

R

R

E

D

S

C

K

E

N

N

I

I

I

Y

G

G

I

V

K
|
K

E

E

S

A

N

S

K

G

N

N

F

I

E

D

Q

K

V

C

S

V

W

D

V

L

L

L

H

A

L

H

N

E

G

P

R

R

D

M

F

M

L

L

V

I

Y

-

S

S

G

G

I

E

E

D

T

A

L

I

C

N

Y

Y

P

P

M

I

L

L

C

S

L

N

G

G

A

K

G

G

H

V

V
x
I

S

S

A

V

T

T

A

S

N

N

L

L

P

P

R

D

E

M

V

I

A

S

R

A

L

L

Y

T

D

H

L

F

T

A

A

L

A

D

G

E

R

N

W

Y

E

K

E

E

A

A

R

K

D

K

L

I

H

N

Y

D

Q

E

L

L

L

Y

E

N

L

I

N

N

D

K

V

I

L

L

F

F

V

C

E

E

T

S

N

N

P

P

G

I

P

P

L

I

K

K

Y

T

A

A

M

M

G

Y

L

L

A

A

G

G

M

L

I

I

H

E

P

S

-

L

R

E

L

F

R

R

P

L

P

P

L

L

C

C

E

S

P

P

S

S

P

K

E

E

N

N

R

F

E

A

K

K

I

I

R

E

T

E

V

V

L

M

A

K

K

K

Y

Y

G

K

L

I

active site: T45 (= T50), Y109 (= Y113), Y135 (= Y139), R140 (= R144), K164 (= K168), I205 (≠ V210)
binding lysine: S49 (≠ P54), A50 (≠ S55), L52 (= L57), H54 (≠ L59), H57 (≠ R62), N82 (= N86), E86 (= E90), Y108 (= Y112)

2atsA Dihydrodipicolinate synthase co-crystallised with (s)-lysine
40% identity, 95% coverage: 8:298/305 of query aligns to 2:292/292 of 2atsA

query
sites
2atsA

L

F

K

T

G

G

S

S

I

I

V

V

P

A

L

I

V

V

T

T

P

P

F

M

D

D

E

E

E

K

G

G

R

N

F

V

D

C

E

R

K

A

T

S

F

L

Q

K

R

K

L

L

I

I

D

D

F

Y

Q

H

I

V

E

A

S

S

G

G

S

T

H

S

G

A

I

I

S

V

C

S

T

V

G

G

T
|
T

T

T

G

G

E

E

P

S

S
x
A

S

T

L
|
L

S

N

L
x
H

E

D

E

E

R
x
H

E

A

Y

D

V

V

I

V

E

M

T

M

A

T

I

L

R

D

H

L

V

A

G

D

G

G

R

R

V

I

P

P

V

V

V

I

P

A

G

G

T

T

G

G

T

A

N
|
N

N

A

Y

T

E

A

E
|
E

T

A

L

I

R

S

L

L

T

T

R

Q

H

R

A

F

Q

N

R

D

L

S

G

G

A

I

D

V

A

G

A

C

L

L

V

T

I

V

V

T

P

P

Y
|
Y

N

N

R

R

P

P

S

S

Q

Q

E

E

G

G

L

L

Y

Y

R

Q

Y

H

F

F

R

K

A

A

L

I

A

A

D

E

E

H

V

T

D

D

I

L

P

P

I

Q

I

I

I

L

Y
|
Y

N

N

I

V

P

P

G

S

R
|
R

T

T

A

G

T

C

N

D

M

L

E

L

P

P

S

E

T

T

V

V

A

G

R

R

L

L

R

A

R

K

E

-

S

V

K

K

N

N

I

I

G

G

I

I

K
|
K

E

E

S

A

N

T

K

G

N

N

F

L

E

T

Q

R

V

V

S

N

W

Q

V

I

L

K

H

E

L

L

N

V

G

S

R

D

D

D

F

F

L

V

V

L

Y

L

S

S

G

G

I

D

E

D

T

A

L

S

C

A

Y

L

P

D

M

F

L

M

C

Q

L

L

G

G

A

H

G

G

H

V

V
x
I

S

S

A

V

T

T

A

A

N

N

L

V

L

A

P

A

R

R

E

D

V

M

A

A

R

Q

L

M

Y

C

D

K

L

L

T

A

A

A

A

E

G

G

R

H

W

F

E

A

E

E

A

A

R

R

D

V

L

I

H

N

Y

Q

Q

R

L

L

L

M

E

P

L

L

N

H

D

N

V

K

L

L

F

F

V

V

E

E

T

P

N

N

P

P

G

I

P

P

L

V

K

K

Y

W

A

A

M

C

G

K

L

E

A

L

G

G

M

L

I

V

H

A

P

T

-

D

R

T

L

L

R

R

P

L

P

P

L

M

C

T

E

P

P

I

S

T

P

D

E

S

N

G

R

R

E

E

K

T

I

V

R

R

T

A

V

A

L

L

A

K

K

H

Y

A

G

G

L

L

active site: T44 (= T50), Y107 (= Y113), Y133 (= Y139), R138 (= R144), K161 (= K168), I203 (≠ V210)
binding d-lysine: A49 (≠ S55), L51 (= L57), H53 (≠ L59), H56 (≠ R62), N80 (= N86), E84 (= E90), Y106 (= Y112)

P0A6L2 4-hydroxy-tetrahydrodipicolinate synthase; HTPA synthase; EC 4.3.3.7 from Escherichia coli (strain K12) (see 7 papers)
40% identity, 95% coverage: 8:298/305 of query aligns to 2:292/292 of P0A6L2

query
sites
P0A6L2

L

F

K

T

G

G

S

S

I

I

V

V

P

A

L

I

V

V

T

T

P

P

F

M

D

D

E

E

E

K

G

G

R

N

F

V

D

C

E

R

K

A

T

S

F

L

Q

K

R

K

L

L

I

I

D

D

F

Y

Q

H

I

V

E

A

S

S

G

G

S

T

H

S

G

A

I

I

S

V

C

S

T

V

G

G

T
|
T

G

G

E

E

P

S

S

A

S

T

L

L

S

N

L

H

E

D

E

E

R

H

E

A

Y

D

V

V

I

V

E

M

T

M

A

T

I

L

R

D

H

L

V

A

G

D

G

G

R

R

V

I

P

P

V

V

V

I

P

A

G

G

T

T

G

G

T

A

N

N

N

A

Y

T

E

A

E

E

T

A

L

I

R

S

L

L

T

T

R

Q

H

R

A

F

Q

N

R

D

L

S

G

G

A

I

D

V

A

G

A

C

L

L

V

T

I

V

V

T

P

P

Y

Y

Y
|
Y

N

N

R

R

P

P

S

S

Q

Q

E

E

G

G

L

L

Y

Y

R

Q

Y

H

F

F

R

K

A

A

L

I

A

A

D

E

E

H

V

T

D

D

I

L

P

P

I

Q

I

I

I

L

Y
|
Y

N

N

I

V

P

P

G

S

R
|
R

T

T

A

G

T

C

N

D

M

L

E

L

P

P

S

E

T

T

V

V

A

G

R

R

L

L

R

A

R

K

E

-

S

V

K

K

N

N

I

I

G

G

I

I

K
|
K

E

E

S

A

N

T

K

G

N

N

F

L

E

T

Q

R

V

V

S

N

W

Q

V

I

L

K

H

E

L

L

N

V

G

S

R

D

D

D

F

F

L

V

V

L

Y

L

S

S

G

G

I

D

E

D

T

A

L

S

C

A

Y

L

P

D

M

F

L

M

C

Q

L
|
L

G

G

A

H

G

G

H

V

V
x
I

S

S

A

V

T

T

A

A

N

N

L

V

L

A

P

A

R

R

E

D

V

M

A

A

R

Q

L

M

Y

C

D

K

L

L

T

A

A

A

A

E

G

G

R

H

W

F

E

A

E

E

A

A

R

R

D

V

L

I

H

N

Y

Q

Q

R

L

L

L

M

E

P

L

L

N

H

D

N

V

K

L

L

F

F

V

V

E

E

T

P

N

N

P

P

G

I

P

P

L

V

K

K

Y

W

A

A

M

C

G

K

L

E

A

L

G

G

M

L

I

V

H

A

P

T

-

D

R

T

L

L

R

R

P

L

P

P

L

M

C

T

E

P

P

I

S

T

P

D

E

S

N

G

R

R

E

E

K

T

I

V

R

R

T

A

V

A

L

L

A

K

K

H

Y

A

G

G

L

L

T44 (= T50) mutation to S: 8% of wild-type activity. 4-fold decrease in affinity for pyruvate, but nearly no change in that for (S)-ASA.; mutation to V: Reduced kcat by 99.9%.
T45 (= T51) binding pyruvate
Y107 (= Y113) mutation to F: Reduced kcat by 90%.; mutation to W: Reduced activity by 95%. Reduced affinity for both substrates. Exists as a mixture of monomer, dimer and tetramer in solution. Has significantly lower thermal stability than the wild-type enzyme.
Y133 (= Y139) mutation to F: Reduced kcat by 99.7%. Reduced affinity for both substrates.
R138 (= R144) mutation R->A,H: Strongly increased KM for L-aspartate 4-semialdehyde. No effect on KM for pyruvate. Reduced activity by 99.7%.
K161 (= K168) mutation to A: 0.1% of wild-type activity. 3-fold decrease in affinity for pyruvate, and 2-fold decrease in that for (S)-ASA.; mutation to R: 0.35% of wild-type activity. 3-fold decrease in affinity for pyruvate, but nearly no change in that for (S)-ASA.
L197 (= L204) mutation L->Y,D: 1.4 to 2.5% of wild-type activity. Decrease in affinity for pyruvate, but nearly no change in that for (S)-ASA. Exists as a dimer in solution.
I203 (≠ V210) binding pyruvate

7kkdB Dihydrodipicolinate synthase (dhdps) from c.Jejuni, n84a mutant with pyruvate bound in the active site and r,r-bislysine bound at the allosteric site
40% identity, 97% coverage: 2:297/305 of query aligns to 8:303/306 of 7kkdB

query
sites
7kkdB

S

S

T

M

D

D

I

K

D

N

R

I

L

I

K

I

G

G

S

A

I

M

V

T

P

A

L

L

V

I

T

T

P

P

F

F

D

-

E

K

E

N

G

G

R

K

F

V

D

D

E

E

K

Q

T

S

F

Y

Q

A

R

R

L

L

I

I

D

K

F

R

Q

Q

I

I

E

E

S

N

G

G

S

I

H

D

G

A

I

V

S

V

C

P

T

V

G

G

T

T

G

G

E

E

P
x
S

S

A

S

T

L
|
L

S

T

L

H

E

E

R
x
H

E

R

Y

T

V

C

I

I

E

E

T

I

A

A

I

V

R

E

H

T

V

C

G

K

G

G

-

T

R

K

V

V

P

K

V

V

V

L

P

A

G

G

T

A

G

G

T

S

N

A

Y

T

E

H

E
|
E

T

A

L

V

R

G

L

L

T

A

R

K

H

F

A

A

Q

K

R

E

L

H

G

G

A

A

D

D

A

G

A

I

L

L

V

S

I

V

V

A

P

P

Y
|
Y

Y

Y

N

N

R

K

P

P

S

T

Q

Q

E

Q

G

G

L

L

Y

Y

R

E

Y

H

F

Y

R

K

A

A

L

I

A

A

D

Q

E

S

V

V

D

D

I

I

P

P

I

V

I

L

Y

Y

N

N

I

V

P

P

G

G

R

R

T

T

A

G

T

C

N

E

M

I

E

S

P

T

S

D

T

T

V

I

A

I

R

K

L

L

R

F

R

R

E

D

S

C

K

E

N

N

I

I

I

Y

G

G

I

V

K

K

E

E

S

A

N

S

K

G

N

N

F

I

E

D

Q

K

V

C

S

V

W

D

V

L

L

L

H

A

L

H

N

E

G

P

R

R

D

M

F

M

L

L

V

I

Y

-

S

S

G

G

I

E

E

D

T

A

L

I

C

N

Y

Y

P

P

M

I

L

L

C

S

L

N

G

G

A

K

G

G

H

V

V

I

S

S

A

V

T

T

A

S

N

N

L

L

P

P

R

D

E

M

V

I

A

S

R

A

L

L

Y

T

D

H

L

F

T

A

A

L

A

D

G

E

R

N

W

Y

E

K

E

E

A

A

R

K

D

K

L

I

H

N

Y

D

Q

E

L

L

L

Y

E

N

L

I

N

N

D

K

V

I

L

L

F

F

V

C

E

E

T

S

N

N

P

P

G

I

P

P

L

I

K

K

Y

T

A

A

M

M

G

Y

L

L

A

A

G

G

M

L

I

I

H

E

P

S

-

L

R

E

L

F

R

R

P

L

P

P

L

L

C

C

E

S

P

P

S

S

P

K

E

E

N

N

R

F

E

A

K

K

I

I

R

E

T

E

V

V

L

M

A

K

K

K

Y

Y

G

K

L

I

binding (2R,5R)-2,5-diamino-2,5-bis(4-aminobutyl)hexanedioic acid: S59 (≠ P54), L62 (= L57), H67 (≠ R62), E96 (= E90), Y118 (= Y112)

5t25A Kinetic, spectral and structural characterization of the slow binding inhibitor acetopyruvate with dihydrodipicolinate synthase from escherichia coli.
40% identity, 95% coverage: 8:298/305 of query aligns to 3:293/293 of 5t25A

query
sites
5t25A

L

F

K

T

G

G

S

S

I

I

V

V

P

A

L

I

V

V

T

T

P

P

F

M

D

D

E

E

E

K

G

G

R

N

F

V

D

C

E

R

K

A

T

S

F

L

Q

K

R

K

L

L

I

I

D

D

F

Y

Q

H

I

V

E

A

S

S

G

G

S

T

H

S

G

A

I

I

S

V

C

S

T

V

G

G

T
|
T

T

T

G

G

E

E

P

S

S
x
A

S

T

L
|
L

S

N

L
x
H

E

D

E

E

R

H

E

A

Y

D

V

V

I

V

E

M

T

M

A

T

I

L

R

D

H

L

V

A

G

D

G

G

R

R

V

I

P

P

V

V

V

I

P

A

G

G

T

T

G

G

T

A

N
|
N

N

A

Y

T

E

A

E
|
E

T

A

L

I

R

S

L

L

T

T

R

Q

H

R

A

F

Q

N

R

D

L

S

G

G

A

I

D

V

A

G

A

C

L

L

V

T

I

V

V

T

P

P

Y
|
Y

N

N

R

R

P

P

S

S

Q

Q

E

E

G

G

L

L

Y

Y

R

Q

Y

H

F

F

R

K

A

A

L

I

A

A

D

E

E

H

V

T

D

D

I

L

P

P

I

Q

I

I

I

L

Y
|
Y

N

N

I

V

P

P

G

S

R
|
R

T

T

A

G

T

C

N

D

M

L

E

L

P

P

S

E

T

T

V

V

A

G

R

R

L

L

R

A

R

K

E

-

S

V

K

K

N

N

I

I

G

G

I

I

K
|
K

E

E

S

A

N

T

K

G

N

N

F

L

E

T

Q

R

V

V

S

N

W

Q

V

I

L

K

H

E

L

L

N

V

G

S

R

D

D

D

F

F

L

V

V

L

Y

L

S

S

G

G

I

D

E

D

T

A

L

S

C

A

Y

L

P

D

M

F

L

M

C

Q

L

L

G

G

A

H

G

G

H

V

V
x
I

S

S

A

V

T

T

A

A

N

N

L

V

L

A

P

A

R

R

E

D

V

M

A

A

R

Q

L

M

Y

C

D

K

L

L

T

A

A

A

A

E

G

G

R

H

W

F

E

A

E

E

A

A

R

R

D

V

L

I

H

N

Y

Q

Q

R

L

L

L

M

E

P

L

L

N

H

D

N

V

K

L

L

F

F

V

V

E

E

T

P

N

N

P

P

G

I

P

P

L

V

K

K

Y

W

A

A

M

C

G

K

L

E

A

L

G

G

M

L

I

V

H

A

P

T

-

D

R

T

L

L

R

R

P

L

P

P

L

M

C

T

E

P

P

I

S

T

P

D

E

S

N

G

R

R

E

E

K

T

I

V

R

R

T

A

V

A

L

L

A

K

K

H

Y

A

G

G

L

L

active site: T45 (= T50), Y108 (= Y113), Y134 (= Y139), R139 (= R144), K162 (= K168), I204 (≠ V210)
binding lysine: A50 (≠ S55), L52 (= L57), H54 (≠ L59), N81 (= N86), E85 (= E90), Y107 (= Y112)

6u01B Dihydrodipicolinate synthase (dhdps) from c.Jejuni, n84d mutant with pyruvate bound in the active site (see paper)
40% identity, 94% coverage: 10:297/305 of query aligns to 6:293/296 of 6u01B

query
sites
6u01B

G

G

S

A

I

M

V

T

P

A

L

L

V

I

T

T

P

P

F

F

D

-

E

K

E

N

G

G

R

K

F

V

D

D

E

E

K

Q

T

S

F

Y

Q

A

R

R

L

L

I

I

D

K

F

R

Q

Q

I

I

E

E

S

N

G

G

S

I

H

D

G

A

I

V

S

V

C

P

T

V

G

G

T
|
T

T

T

G

G

E

E

P

S

S

A

S

T

L

L

S

T

L

H

E

E

R

H

E

R

Y

T

V

C

I

I

E

E

T

I

A

A

I

V

R

E

H

T

V

C

G

K

G

G

-

T

R

K

V

V

P

K

V

V

V

L

P

A

G

G

T

A

G

G

T

S

N

D

N

A

Y

T

E

H

E

E

T

A

L

V

R

G

L

L

T

A

R

K

H

F

A

A

Q

K

R

E

L

H

G

G

A

A

D

D

A

G

A

I

L

L

V

S

I

V

V

A

P

P

Y

Y

Y
|
Y

N

N

R

K

P

P

S

T

Q

Q

E

Q

G

G

L

L

Y

Y

R

E

Y

H

F

Y

R

K

A

A

L

I

A

A

D

Q

E

S

V

V

D

D

I

I

P

P

I

V

I

L

Y
|
Y

N

N

I

V

P

P

G

G

R
|
R

T

T

A

G

T

C

N

E

M

I

E

S

P

T

S

D

T

T

V

I

A

I

R

K

L

L

R

F

R

R

E

D

S

C

K

E

N

N

I

I

I

Y

G

G

I

V

K
|
K

E

E

S

A

N

S

K

G

N

N

F

I

E

D

Q

K

V

C

S

V

W

D

V

L

L

L

H

A

L

H

N

E

G

P

R

R

D

M

F

M

L

L

V

I

Y

-

S
|
S

G

G

I
x
E

E
x
D

T

A

L

I

C
x
N

Y

Y

P

P

M

I

L

L

C

S

L

N

G

G

A

K

G

G

H

V

V
x
I

S

S

A

V

T

T

A

S

N

N

L

L

P

P

R

D

E

M

V

I

A

S

R

A

L

L

Y

T

D

H

L

F

T

A

A

L

A

D

G

E

R

N

W

Y

E

K

E

E

A

A

R

K

D

K

L

I

H

N

Y

D

Q

E

L

L

L

Y

E

N

L

I

N

N

D

K

V

I

L

L

F

F

V

C

E

E

T

S

N

N

P

P

G

I

P

P

L

I

K

K

Y

T

A

A

M

M

G

Y

L

L

A

A

G

G

M

L

I

I

H

E

P

S

-

L

R

E

L

F

R

R

P

L

P

P

L

L

C

C

E

S

P

P

S

S

P

K

E

E

N

N

R

F

E

A

K

K

I

I

R

E

T

E

V

V

L

M

A

K

K

K

Y

Y

G

K

L

I

active site: T45 (= T50), Y109 (= Y113), Y135 (= Y139), R140 (= R144), K164 (= K168), I205 (≠ V210)
binding magnesium ion: S187 (= S192), E189 (≠ I194), D190 (≠ E195), N193 (≠ C198), I205 (≠ V210)

3i7sA Dihydrodipicolinate synthase mutant - k161a - with the substrate pyruvate bound in the active site. (see paper)
40% identity, 95% coverage: 8:298/305 of query aligns to 2:292/292 of 3i7sA

query
sites
3i7sA

L

F

K

T

G

G

S

S

I

I

V

V

P
x
A

L

I

V

V

T

T

P

P

F

M

D

D

E

E

E

K

G

G

R

N

F

V

D

C

E

R

K

A

T

S

F

L

Q

K

R

K

L

L

I

I

D

D

F

Y

Q

H

I

V

E

A

S

S

G

G

S

T

H

S

G

A

I

I

S

V

C

S

T

V

G

G

T
|
T

G

G

E

E

P

S

S

A

S

T

L

L

S

N

L

H

E

D

E

E

R

H

E

A

Y

D

V

V

I

V

E

M

T

M

A

T

I

L

R

D

H

L

V

A

G

D

G

G

R

R

V

I

P

P

V

V

V

I

P

A

G

G

T

T

G

G

T

A

N

N

N

A

Y

T

E

A

E

E

T

A

L

I

R

S

L

L

T

T

R

Q

H

R

A

F

Q

N

R

D

L

S

G

G

A

I

D

V

A

G

A

C

L

L

V

T

I

V

V

T

P

P

Y

Y

Y
|
Y

N

N

R

R

P

P

S

S

Q

Q

E

E

G

G

L

L

Y

Y

R

Q

Y

H

F

F

R

K

A

A

L

I

A

A

D

E

E

H

V

T

D

D

I

L

P

P

I

Q

I

I

I

L

Y
|
Y

N

N

I

V

P

P

G

S

R
|
R

T

T

A

G

T

C

N

D

M

L

E

L

P

P

S

E

T

T

V

V

A

G

R

R

L

L

R

A

R

K

E

-

S

V

K

K

N

N

I

I

G

G

I

I

K
x
R

E

E

S

A

N

T

K

G

N

N

F

L

E

T

Q

R

V

V

S

N

W

Q

V

I

L

K

H

E

L

L

N

V

G

S

R

D

D

D

F

F

L

V

V

L

Y

L

S

S

G

G

I

D

E

D

T

A

L

S

C

A

Y

L

P

D

M

F

L

M

C

Q

L

L

G

G

A

H

G

G

H

V

V
x
I

S

S

A

V

T

T

A

A

N

N

L

V

L

A

P

A

R

R

E

D

V

M

A

A

R

Q

L

M

Y

C

D

K

L

L

T

A

A

A

A

E

G

G

R

H

W

F

E

A

E

E

A

A

R

R

D

V

L

I

H

N

Y

Q

Q

R

L

L

L

M

E

P

L

L

N

H

D

N

V

K

L

L

F

F

V

V

E

E

T

P

N

N

P

P

G

I

P

P

L

V

K

K

Y

W

A

A

M

C

G

K

L

E

A

L

G

G

M

L

I

V

H

A

P

T

-

D

R

T

L

L

R

R

P

L

P

P

L

M

C

T

E

P

P

I

S

T

P

D

E

S

N

G

R

R

E

E

K

T

I

V

R

R

T

A

V

A

L

L

A

K

K

H

Y

A

G

G

L

L

active site: T44 (= T50), Y107 (= Y113), Y133 (= Y139), R138 (= R144), R161 (≠ K168), I203 (≠ V210)
binding pyruvic acid: A8 (≠ P14), T44 (= T50), T45 (= T51), Y133 (= Y139)

Q07607 4-hydroxy-tetrahydrodipicolinate synthase; HTPA synthase; Protein MosA; EC 4.3.3.7 from Rhizobium meliloti (Ensifer meliloti) (Sinorhizobium meliloti) (see paper)
40% identity, 95% coverage: 8:298/305 of query aligns to 2:291/292 of Q07607

query
sites
Q07607

L

F

K

E

G

G

S

S

I

I

V

T

P

A

L

L

V

V

T

T

P

P

F

F

D

A

E

D

G

-

R

R

F

I

D

D

E

E

K

V

T

A

F

L

Q

H

R

D

L

L

I

V

D

E

F

W

Q

Q

I

I

E

E

S

E

G

G

S

S

H

F

G

G

I

L

S

V

C

P

T

C

G

G

T

T

G

G

E

E

P

S

S

P

S

T

L

L

S

S

L

K

E

S

E

E

R

H

E

E

Y

Q

V

V

I

V

E

E

T

I

A

T

I

I

R

K

H

T

V

A

G

N

G

G

R

R

V

V

P

P

V

V

V

I

P

A

G

G

T

A

G

G

T

S

N

N

N

S

Y

T

E

A

E

E

T

A

L

I

R

A

L

F

T

V

R

R

H

H

A

A

Q

Q

R

N

L

A

G

G

A

A

D

D

A

G

A

V

L

L

V

I

I

V

V

S

P

P

Y

Y

N

N

R

K

P

P

S

T

Q

Q

E

E

G

G

L

I

Y

Y

R

Q

Y

H

F

F

R

K

A

A

L

I

A

D

D

A

E

A

V

S

D

T

I

I

P

P

I

I

I

V

Y

Y

N

N

I

I

P

P

G

G

R

R

T

S

A

A

T

I

N

E

M

I

E

H

P

V

S

E

T

T

V

L

A

A

R

R

L

I

R

F

R

E

E

D

S

C

K

P

N

N

I

V

I

K

G

G

I

V

K
|
K

E

D

S

A

N

T

K

G

N

N

F

L

E

L

Q

R

V

P

S

S

W

L

V

E

L

R

H

M

L

A

N

C

G

G

R

E

D

D

F

F

L

N

V

L

Y

L

S

T

G

G

I

E

E

D

T

G

L

T

C

A

Y

L

P

G

M

Y

L

M

C

A

L

H

G

G

A

H

G

G

H

C

V

I

S

S

A

V

T

T

A

A

N

N

L

V

L

A

P

P

R

A

E

L

V

C

A

A

R

D

L

F

Y

Q

D

Q

L

A

T

C

A

L

A

N

G

G

R

D

W

F

E

A

A

A

R

L

D

K

L

L

H

Q

Y

D

Q

R

L

L

L

M

E

P

L

L

N

H

D

R

V

A

L

L

F

F

V

L

E

E

T

T

N

N

P

P

G

A

P

G

L

A

K

K

Y

Y

A

A

M

L

G

Q

L

R

A

L

G

G

M

R

I

M

H

R

P

G

R

D

L

L

R

R

P

L

P

P

L

L

C

V

E

T

P

I

S

S

P

P

E

S

N

F

R

Q

E

E

K

E

I

I

R

D

T

D

V

A

L

M

A

R

K

H

Y

A

G

G

L

I

L

L

K161 (= K168) active site, Schiff-base intermediate with substrate

Q8UGL3 4-hydroxy-tetrahydrodipicolinate synthase; HTPA synthase; EC 4.3.3.7 from Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58)) (see paper)
39% identity, 95% coverage: 8:298/305 of query aligns to 2:293/294 of Q8UGL3

query
sites
Q8UGL3

L

F

K

K

G

G

S

S

I

I

V

P

P

A

L

L

V

I

T

T

P

P

F

F

D

T

E

D

E

N

G

G

R

S

F

V

D

D

E

E

K

K

T

A

F

F

Q

A

R

A

L

H

I

V

D

E

F

W

Q

Q

I

I

E

A

S

E

G

G

S

S

H

N

G

G

I

L

S

V

C

P

T

V

G

G

T

T

T
|
T

G

G

E

E

P

S

S
x
P

S

T

L

L

S

S

L

H

E

D

E

E

R
x
H

E

K

Y

R

V

V

I

V

E

E

T

L

A

C

I

I

R

E

H

V

V

A

G

A

G

K

R

R

V

V

P

P

V

V

V

I

P

A

G

G

T

A

G

G

T

S

N
|
N

N

N

Y

T

E

D

E
|
E

T

A

L

I

R

E

L

L

T

A

R

L

H

H

A

A

Q

Q

R

E

L

A

G

G

A

A

D

D

A

A

A

L

L

L

V

V

I

V

V

T

P

P

Y
|
Y

Y

Y

N

N

R

K

P

P

S

T

Q

Q

E

K

G

G

L

L

Y

F

R

A

Y

H

F

F

R

S

A

A

L

V

A

A

D

E

E

A

V

V

D

K

I

L

P

P

I

I

I

V

I

I

Y

Y

N

N

I

I

P

P

G

P

R

R

T

S

A

V

T

V

N

D

M

M

E

S

P

P

S

E

T

T

V

M

A

G

R

A

L

L

R

V

R

K

E

A

S

H

K

K

N

N

I

I

G

G

I

V

K
|
K

E

D

S

A

N

T

K

G

N

K

F

L

E

D

Q

R

V

V

S

S

W

E

V

Q

L

R

H

I

L

S

N

C

G

G

R

K

D

D

F

F

L

V

V

Q

Y

L

S

S

G

G

I

E

E

D

T

G

L

T

C

A

Y

L

P

G

M

F

L

N

C

A

L

H

G

G

A

V

G

G

H

C

V

I

S

S

A

V

T

T

A

A

N

N

L

V

L

A

P

P

R

R

E

L

V

C

A

S

R

E

L

F

Y

Q

D

A

L

A

T

M

A

L

A

A

G

G

R

D

W

Y

E

A

E

K

A

A

R

L

D

E

L

Y

H

Q

Y

D

Q

R

L

L

L

M

E

P

L

L

N

H

D

R

V

A

L

I

F

F

V

M

E

E

T

P

N

G

P

V

G

C

P

G

L

T

K

K

Y

Y

A

A

M

L

G

S

L

K

A

T

G

R

M

G

I

G

H

N

P

R

R

R

L

V

R

R

P

S

P

P

L

L

C

M

E

S

P

T

-

L

S

E

P

P

E

A

N

T

R

E

E

A

K

A

I

I

R

D

T

A

V

A

L

L

A

K

K

H

Y

A

G

G

L

L

L

M

T45 (= T51) binding pyruvate
P49 (≠ S55) L-lysine inhibitor binding; via carbonyl oxygen
H56 (≠ R62) L-lysine inhibitor binding
N80 (= N86) L-lysine inhibitor binding
E84 (= E90) L-lysine inhibitor binding
Y106 (= Y112) L-lysine inhibitor binding
K162 (= K168) active site, Schiff-base intermediate with substrate

Query Sequence

>WP_013075572.1 NCBI__GCF_000092905.1:WP_013075572.1
MSTDIDRLKGSIVPLVTPFDEEGRFDEKTFQRLIDFQIESGSHGISCTGTTGEPSSLSLE
EREYVIETAIRHVGGRVPVVPGTGTNNYEETLRLTRHAQRLGADAALVIVPYYNRPSQEG
LYRYFRALADEVDIPIIIYNIPGRTATNMEPSTVARLRRESKNIIGIKESNKNFEQVSWV
LHLNGRDFLVYSGIETLCYPMLCLGGAGHVSATANLLPREVARLYDLTAAGRWEEARDLH
YQLLELNDVLFVETNPGPLKYAMGLAGMIHPRLRPPLCEPSPENREKIRTVLAKYGLLAK
ERSES

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory