SitesBLAST
Comparing WP_013088370.1 NCBI__GCF_000092885.1:WP_013088370.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
6lpxA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with 2-oxoglutarate (2-og) (see paper)
23% identity, 28% coverage: 197:574/1368 of query aligns to 49:364/466 of 6lpxA
- binding 2-oxoglutaric acid: R333 (= R543), T337 (= T546), K348 (= K557)
- binding flavin-adenine dinucleotide: P75 (= P223), Q76 (≠ R224), G77 (= G225), G78 (= G226), N79 (≠ G227), T80 (= T228), G81 (= G229), M82 (≠ Y230), G85 (= G233), S86 (≠ A234), L139 (≠ P296), G140 (≠ T297), A141 (≠ S298), C145 (≠ S302), G149 (= G306), N150 (= N307), A152 (= A309), T153 (≠ M310), G157 (= G314), G207 (= G412), I212 (= I417)
Sites not aligning to the query:
6lpuA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with l-2-hydroxyglutarate (l-2-hg) (see paper)
23% identity, 28% coverage: 197:574/1368 of query aligns to 49:364/466 of 6lpuA
- binding flavin-adenine dinucleotide: P75 (= P223), G77 (= G225), G78 (= G226), N79 (≠ G227), T80 (= T228), G81 (= G229), G85 (= G233), S86 (≠ A234), L139 (≠ P296), G140 (≠ T297), A141 (≠ S298), C145 (≠ S302), H146 (≠ C303), G148 (= G305), G149 (= G306), N150 (= N307), A152 (= A309), T153 (≠ M310), A155 (= A312), E206 (= E411), G207 (= G412), I211 (≠ L416), I212 (= I417)
- binding (2s)-2-hydroxypentanedioic acid: R333 (= R543), T337 (= T546), K348 (= K557)
Sites not aligning to the query:
6lpqA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-malate (d-mal) (see paper)
23% identity, 28% coverage: 197:574/1368 of query aligns to 49:364/466 of 6lpqA
- binding flavin-adenine dinucleotide: P75 (= P223), G77 (= G225), G78 (= G226), N79 (≠ G227), T80 (= T228), G81 (= G229), G85 (= G233), S86 (≠ A234), L139 (≠ P296), G140 (≠ T297), A141 (≠ S298), C145 (≠ S302), H146 (≠ C303), G149 (= G306), N150 (= N307), A152 (= A309), T153 (≠ M310), A155 (= A312), G157 (= G314), E206 (= E411), G207 (= G412), I211 (≠ L416), I212 (= I417)
- binding d-malate: M82 (≠ Y230), R333 (= R543), T337 (= T546), K348 (= K557)
Sites not aligning to the query:
6lppA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-2-hydroxyglutarate (d-2-hg) (see paper)
23% identity, 28% coverage: 197:574/1368 of query aligns to 49:364/466 of 6lppA
- binding (2R)-2-hydroxypentanedioic acid: R333 (= R543), T337 (= T546), K348 (= K557)
- binding flavin-adenine dinucleotide: P75 (= P223), G77 (= G225), G78 (= G226), N79 (≠ G227), T80 (= T228), G81 (= G229), M82 (≠ Y230), G85 (= G233), S86 (≠ A234), L139 (≠ P296), G140 (≠ T297), A141 (≠ S298), C145 (≠ S302), G149 (= G306), N150 (= N307), A152 (= A309), T153 (≠ M310), A155 (= A312), G157 (= G314), G207 (= G412), I212 (= I417)
Sites not aligning to the query:
6lpnB Crystal structure of human d-2-hydroxyglutarate dehydrogenase in apo form (see paper)
23% identity, 28% coverage: 197:574/1368 of query aligns to 50:365/467 of 6lpnB
- binding flavin-adenine dinucleotide: P76 (= P223), G78 (= G225), G79 (= G226), N80 (≠ G227), T81 (= T228), G82 (= G229), M83 (≠ Y230), G86 (= G233), S87 (≠ A234), L140 (≠ P296), A142 (≠ S298), C146 (≠ S302), H147 (≠ C303), G150 (= G306), N151 (= N307), A153 (= A309), T154 (≠ M310), G208 (= G412), I212 (≠ L416), I213 (= I417)
Sites not aligning to the query:
Q8N465 D-2-hydroxyglutarate dehydrogenase, mitochondrial; EC 1.1.99.39 from Homo sapiens (Human) (see 7 papers)
23% identity, 28% coverage: 197:574/1368 of query aligns to 102:417/521 of Q8N465
- S109 (≠ E204) to W: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs142050154
- N127 (≠ I222) to K: in D2HGA1; uncertain significance; complete loss of catalytic activity; dbSNP:rs762857195
- G131 (= G226) to V: in D2HGA1; uncertain significance; complete loss of catalytic activity
- I147 (= I244) to S: in D2HGA1; uncertain significance; severe phenotype; almost complete loss of catalytic activity; dbSNP:rs121434361
- M153 (= M257) to T: in D2HGA1; uncertain significance; significant loss of catalytic activity; to V: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs1432270139
- C172 (≠ V276) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs773735172
- P189 (≠ A293) to L: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs587783517
- A205 (= A309) to V: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs750889931
- A231 (≠ P335) to V: in D2HGA1; uncertain significance; significant loss of catalytic activity
- G233 (= G337) to S: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs374535734
- D375 (≠ S532) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs267606759
- R386 (= R543) binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- T390 (= T546) binding (R)-2-hydroxyglutarate; binding (R)-malate; mutation to A: Significantly reduced catalytic activity.
- V399 (≠ A555) to M: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746519212
- K401 (= K557) binding (R)-2-hydroxyglutarate; binding (R)-malate; mutation to A: Loss of catalytic activity.
Sites not aligning to the query:
- 15 R → G: in dbSNP:rs4675887
- 169:521 natural variant: Missing (in D2HGA1; uncertain significance)
- 400:521 natural variant: Missing (in D2HGA1; uncertain significance; complete loss of catalytic activity)
- 419 R → H: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs199908032
- 426 A → T: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs146578303
- 434 binding Zn(2+); H→A: Loss of catalytic activity.
- 436 G → V: slight reduction in catalytic activity
- 439 N → D: in D2HGA1; uncertain significance; mild phenotype; moderate reduction in catalytic activity; dbSNP:rs121434362
- 441 binding Zn(2+); H→A: Loss of catalytic activity.
- 443 binding (R)-2-hydroxyglutarate; N→A: Significantly reduced catalytic activity.
- 444 V → A: in D2HGA1; uncertain significance; severe phenotype; significant reduction in catalytic activity; dbSNP:rs121434360
- 446 A → V: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746956176
- 475 binding Zn(2+); E→A: Loss of catalytic activity.
- 476 binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; H→A: Loss of catalytic activity.
- 477 G → R: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs1453924640
8jdvA Crystal structure of mldhd in complex with 2-ketohexanoic acid (see paper)
26% identity, 29% coverage: 180:571/1368 of query aligns to 25:347/454 of 8jdvA
- binding 2-Ketohexanoic acid: V75 (≠ Y230), R317 (= R543), W321 (vs. gap)
- binding flavin-adenine dinucleotide: P68 (= P223), G70 (= G225), T71 (≠ G226), G72 (= G227), T73 (= T228), G74 (= G229), G78 (= G233), V79 (≠ A234), L90 (≠ T246), P132 (= P296), G133 (≠ T297), A134 (≠ S298), G140 (= G306), M141 (≠ N307), A143 (= A309), T144 (≠ M310), A146 (= A312), S147 (≠ G313), E200 (= E411), G201 (= G412), I206 (= I417), W321 (vs. gap), Y322 (vs. gap)
Sites not aligning to the query:
8jdsA Crystal structure of mldhd in complex with pyruvate (see paper)
25% identity, 29% coverage: 180:571/1368 of query aligns to 25:349/456 of 8jdsA
- binding flavin-adenine dinucleotide: E32 (≠ A187), P68 (= P223), G70 (= G225), T71 (≠ G226), G72 (= G227), T73 (= T228), G74 (= G229), G78 (= G233), V79 (≠ A234), L90 (≠ T246), P132 (= P296), G133 (≠ T297), A134 (≠ S298), G140 (= G306), M141 (≠ N307), A143 (= A309), T144 (≠ M310), A146 (= A312), S147 (≠ G313), E200 (= E411), G201 (= G412), I206 (= I417), W323 (vs. gap)
- binding pyruvic acid: R319 (= R543)
Sites not aligning to the query:
8jdrA Crystal structure of h405a mldhd in complex with d-2-hydroxy-3-methyl- valeric acid (see paper)
25% identity, 29% coverage: 180:571/1368 of query aligns to 25:349/456 of 8jdrA
- binding flavin-adenine dinucleotide: P68 (= P223), G70 (= G225), T71 (≠ G226), G72 (= G227), T73 (= T228), G74 (= G229), G78 (= G233), V79 (≠ A234), L90 (≠ T246), P132 (= P296), G133 (≠ T297), A134 (≠ S298), G140 (= G306), M141 (≠ N307), A143 (= A309), T144 (≠ M310), A146 (= A312), S147 (≠ G313), E200 (= E411), G201 (= G412), I206 (= I417), Y324 (vs. gap)
- binding (2R,3S)-3-methyl-2-oxidanyl-pentanoic acid: R319 (= R543), W323 (vs. gap)
Sites not aligning to the query:
8jdqA Crystal structure of h405a mldhd in complex with d-2-hydroxyisocaproic acid (see paper)
25% identity, 29% coverage: 180:571/1368 of query aligns to 25:349/456 of 8jdqA
- binding (2R)-2-hydroxy-4-methylpentanoic acid: R319 (= R543), W323 (vs. gap)
- binding flavin-adenine dinucleotide: P68 (= P223), G70 (= G225), T71 (≠ G226), G72 (= G227), T73 (= T228), G74 (= G229), G78 (= G233), V79 (≠ A234), L90 (≠ T246), P132 (= P296), G133 (≠ T297), A134 (≠ S298), G140 (= G306), M141 (≠ N307), A143 (= A309), T144 (≠ M310), A146 (= A312), S147 (≠ G313), E200 (= E411), G201 (= G412), I206 (= I417)
Sites not aligning to the query:
8jdoA Crystal structure of h405a mldhd in complex with d-2-hydroxyhexanoic acid (see paper)
25% identity, 29% coverage: 180:571/1368 of query aligns to 25:349/456 of 8jdoA
- binding (2R)-2-hydroxyhexanoic acid: R319 (= R543), W323 (vs. gap)
- binding flavin-adenine dinucleotide: P68 (= P223), G70 (= G225), T71 (≠ G226), G72 (= G227), T73 (= T228), G74 (= G229), G78 (= G233), V79 (≠ A234), L90 (≠ T246), P132 (= P296), G133 (≠ T297), A134 (≠ S298), G140 (= G306), M141 (≠ N307), A143 (= A309), T144 (≠ M310), A146 (= A312), S147 (≠ G313), E200 (= E411), G201 (= G412), I206 (= I417), Y324 (vs. gap)
Sites not aligning to the query:
8jdnA Crystal structure of h405a mldhd in complex with d-2-hydroxyvaleric acid (see paper)
25% identity, 29% coverage: 180:571/1368 of query aligns to 25:349/456 of 8jdnA
- binding flavin-adenine dinucleotide: P68 (= P223), G70 (= G225), T71 (≠ G226), G72 (= G227), T73 (= T228), G74 (= G229), G78 (= G233), V79 (≠ A234), L90 (≠ T246), P132 (= P296), G133 (≠ T297), A134 (≠ S298), G140 (= G306), M141 (≠ N307), A143 (= A309), T144 (≠ M310), A146 (= A312), S147 (≠ G313), E200 (= E411), G201 (= G412), I206 (= I417)
- binding (2R)-2-oxidanylpentanoic acid: R319 (= R543), W323 (vs. gap)
Sites not aligning to the query:
8jdgA Crystal structure of h405a mldhd in complex with d-2-hydroxybutanoic acid (see paper)
25% identity, 29% coverage: 180:571/1368 of query aligns to 25:349/456 of 8jdgA
- binding flavin-adenine dinucleotide: P68 (= P223), G70 (= G225), T71 (≠ G226), G72 (= G227), T73 (= T228), G74 (= G229), G78 (= G233), V79 (≠ A234), L90 (≠ T246), P132 (= P296), G133 (≠ T297), A134 (≠ S298), G140 (= G306), M141 (≠ N307), A143 (= A309), T144 (≠ M310), A146 (= A312), S147 (≠ G313), E200 (= E411), G201 (= G412), I206 (= I417)
- binding (2R)-2-oxidanylbutanoic acid: R319 (= R543)
Sites not aligning to the query:
8jdbA Crystal structure of h405a mldhd in complex with d-2-hydroxyoctanoic acid (see paper)
25% identity, 29% coverage: 180:571/1368 of query aligns to 25:349/456 of 8jdbA
- binding flavin-adenine dinucleotide: P68 (= P223), G70 (= G225), T71 (≠ G226), G72 (= G227), T73 (= T228), G74 (= G229), G78 (= G233), V79 (≠ A234), L90 (≠ T246), P132 (= P296), G133 (≠ T297), A134 (≠ S298), G140 (= G306), M141 (≠ N307), A143 (= A309), T144 (≠ M310), A146 (= A312), S147 (≠ G313), E200 (= E411), G201 (= G412), I206 (= I417), Y324 (vs. gap)
- binding (2R)-2-oxidanyloctanoic acid: V75 (≠ Y230), R319 (= R543), W323 (vs. gap)
Sites not aligning to the query:
8jdtA Crystal structure of mldhd in complex with 2-ketobutanoic acid (see paper)
25% identity, 29% coverage: 180:571/1368 of query aligns to 25:348/455 of 8jdtA
- binding 2-ketobutyric acid: R318 (= R543)
- binding flavin-adenine dinucleotide: P68 (= P223), G70 (= G225), T71 (≠ G226), G72 (= G227), T73 (= T228), G74 (= G229), G78 (= G233), V79 (≠ A234), L90 (≠ T246), P132 (= P296), G133 (≠ T297), A134 (≠ S298), G140 (= G306), M141 (≠ N307), A143 (= A309), T144 (≠ M310), A146 (= A312), S147 (≠ G313), E200 (= E411), G201 (= G412), I206 (= I417), W322 (vs. gap)
Sites not aligning to the query:
8jduA Crystal structure of mldhd in complex with 2-ketovaleric acid (see paper)
25% identity, 29% coverage: 180:571/1368 of query aligns to 25:348/455 of 8jduA
- binding 2-oxopentanoic acid: R318 (= R543), W322 (vs. gap)
- binding flavin-adenine dinucleotide: P68 (= P223), G70 (= G225), T71 (≠ G226), G72 (= G227), T73 (= T228), G74 (= G229), G78 (= G233), V79 (≠ A234), L90 (≠ T246), P132 (= P296), G133 (≠ T297), A134 (≠ S298), G140 (= G306), M141 (≠ N307), A143 (= A309), T144 (≠ M310), A146 (= A312), S147 (≠ G313), E200 (= E411), G201 (= G412), I206 (= I417), W322 (vs. gap)
Sites not aligning to the query:
P39976 D-2-hydroxyglutarate--pyruvate transhydrogenase DLD3; D-2HG--pyruvate transhydrogenase DLD3; (R)-2-hydroxyglutarate--pyruvate transhydrogenase; D-lactate dehydrogenase [cytochrome] 3; D-lactate ferricytochrome C oxidoreductase; D-LCR; EC 1.1.99.40; EC 1.1.2.4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
28% identity, 11% coverage: 189:337/1368 of query aligns to 59:201/496 of P39976
Sites not aligning to the query:
- 17 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
8jdxA Crystal structure of mldhd in complex with 2-ketoisovaleric acid (see paper)
24% identity, 30% coverage: 156:571/1368 of query aligns to 1:348/455 of 8jdxA
- binding flavin-adenine dinucleotide: E32 (≠ A187), P68 (= P223), G70 (= G225), T71 (≠ G226), G72 (= G227), T73 (= T228), G74 (= G229), G78 (= G233), V79 (≠ A234), L90 (≠ T246), P132 (= P296), G133 (≠ T297), A134 (≠ S298), G140 (= G306), M141 (≠ N307), A143 (= A309), T144 (≠ M310), A146 (= A312), S147 (≠ G313), E200 (= E411), G201 (= G412), I206 (= I417), W322 (vs. gap)
- binding 3-methyl-2-oxobutanoic acid: R318 (= R543)
Sites not aligning to the query:
8jdpA Crystal structure of h405a mldhd in complex with d-2-hydroxyisovaleric acid (see paper)
25% identity, 29% coverage: 180:571/1368 of query aligns to 25:348/455 of 8jdpA
- binding flavin-adenine dinucleotide: P68 (= P223), G70 (= G225), T71 (≠ G226), G72 (= G227), T73 (= T228), G74 (= G229), G78 (= G233), V79 (≠ A234), L90 (≠ T246), P132 (= P296), G133 (≠ T297), A134 (≠ S298), G140 (= G306), M141 (≠ N307), A143 (= A309), T144 (≠ M310), A146 (= A312), S147 (≠ G313), E200 (= E411), G201 (= G412), I206 (= I417)
- binding deaminohydroxyvaline: R319 (= R543)
Sites not aligning to the query:
8jdzA Crystal structure of mldhd in complex with 2-keto-3-methylvaleric acid (see paper)
24% identity, 30% coverage: 156:571/1368 of query aligns to 1:348/454 of 8jdzA
- binding (3S)-3-methyl-2-oxopentanoic acid: R318 (= R543), W322 (vs. gap)
- binding flavin-adenine dinucleotide: E32 (≠ A187), P68 (= P223), G70 (= G225), T71 (≠ G226), G72 (= G227), T73 (= T228), G74 (= G229), G78 (= G233), V79 (≠ A234), L90 (≠ T246), P132 (= P296), G133 (≠ T297), A134 (≠ S298), G140 (= G306), M141 (≠ N307), A143 (= A309), T144 (≠ M310), A146 (= A312), S147 (≠ G313), E200 (= E411), G201 (= G412), I206 (= I417), W322 (vs. gap)
Sites not aligning to the query:
Query Sequence
>WP_013088370.1 NCBI__GCF_000092885.1:WP_013088370.1
MNAPQVFDPHGAAAAVAADPEARLREIPYNYTSFSDREIVIRLLGNDAWDALAELRAERR
TGRSARMLYEVLGDIWVVRRNPYLQDDLLDNPKRRALLIEALNHRLAEIEKRRRADLSEH
GDEAGVDRAARVEKLVQAARRAIDQFASEFQQTYDLRRRTTRVLGKVTQKDNIKFDGLSR
VSHVTDATDWRVEYPFVVLTPDSEAEIAGMIKACFELGLTVIPRGGGTGYTGGAVPLTPF
SAVINTEKLEQLGPVEMTQLPGVDRKVATIFSGAGVVTRRVTEAAEQAGFVFAVDPTSLD
ASCVGGNVAMNAGGKKAVLWGTALDNLAWWRMVDPEGNWLEVTRLEHNMGKIHDIEVARF
ELKWFDGNYAPGEKLLRTESLDIKGRVFRKEGLGKDVTDKFLAGLPGVQKEGCDGLITSA
RWVLHKMPAHTRTVCLEFFGQAREAIPSIVEIKDYLFETSRQGGAILAGLEHLDERYLRA
VGYATKSKRNAFPKMVLIGDIVGDDADAVAQATSEVVRMANGKSGEGFVAVSAEARKRFW
LDRSRTAAIAKHTNAFKINEDVVIPLDRMDEYTDGIERINIELSLKNKLQLVDALEAFFK
GGKLPLGKSDDANEIPSAELLEDRVQQALDLLARVRTRWEFVRDKLDLSLREAQHYLVGL
GYESLAEKFADRVDAQPDATVFHLAQDRTIRVSWKQEIRAELRQIFNGGEFKPILDEAQA
IHKQVLRGRVFVALHMHAGDGNVHTNLPVNSDNYEMLQDAHAAVARIMKLARSLDGVISG
EHGIGITKLEFLTDDEIGEFRKYKQRVDPHGRFNAGKLLEGADLRNAYTPSFGLMGYESL
IMQQSDIGAISESIKDCLRCGKCKPVCATHVPRANLLYSPRNKILATSLLVEAFLYEEQT
RRGVSIKHWDEFNDVADHCTVCHKCVTPCPVKIDFGDVSMNMRNLLRKMGKKKFNPGNAA
GMFFLNATNPQTINLARTAMMGVGYKAQRLGNEVLKKFTKKQTAHPPATVGKPAITQQVI
HFMNKKMPGNLPKKTARALLDIEDNKIVPIIRNPKTTTADTEAVFYFPGCGSERLFSQVG
LATQAMLWEAGVQTVLPPGYLCCGYPQRGSGQYDKAEQIVTDNRVLFHRVANTLNYLDIK
TVVVSCGTCYDQLAGYEFEKIFPGCRIIDIHEYLLEKGIKLDGVNGVRYMYHDPCHSPIK
TMDPVKLVNQLMGSEQDGYKIEKNDRCCGESGTLAVTRPDISTQVRFRKEEEIRKGAAKL
RGIPLVAEAGANAINPANASAGSAGAPNGSVLKAGDGPQPGNGNAGSTDVKILTSCPSCL
QGLSRYNEDAGTEADYIVVEMARHVLGENWMADYVQRANNGGIERVLV
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory