SitesBLAST
Comparing WP_013088923.1 NCBI__GCF_000092885.1:WP_013088923.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P09342 Acetolactate synthase 1, chloroplastic; ALS I; Acetohydroxy-acid synthase I; Acetolactate synthase I; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see 2 papers)
43% identity, 95% coverage: 23:581/587 of query aligns to 96:658/667 of P09342
- C161 (= C88) modified: Disulfide link with 307
- P194 (= P121) mutation to Q: In C3; highly resistant to sulfonylurea herbicides.
- C307 (≠ I232) modified: Disulfide link with 161
P09114 Acetolactate synthase 2, chloroplastic; ALS II; Acetohydroxy-acid synthase II; Acetolactate synthase II; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see paper)
43% identity, 95% coverage: 23:581/587 of query aligns to 93:655/664 of P09114
- P191 (= P121) mutation to A: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with L-568.
- W568 (= W500) mutation to L: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with A-191.
P17597 Acetolactate synthase, chloroplastic; AtALS; Acetohydroxy-acid synthase; Protein CHLORSULFURON RESISTANT 1; EC 2.2.1.6 from Arabidopsis thaliana (Mouse-ear cress) (see 8 papers)
42% identity, 95% coverage: 23:581/587 of query aligns to 99:661/670 of P17597
- A122 (≠ S46) mutation to V: Reduced catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
- M124 (≠ L48) mutation to E: Reduced catalytic activity. Resistant to imidazolinone herbicides and reduced sensitivity to sulfonylurea herbicides.; mutation to I: No effect on catalytic activity. Increased resistance to imidazolinone herbicides.
- E144 (= E68) binding
- S186 (= S110) binding
- P197 (= P121) mutation to S: In csr1-1/GH50; resistant to sulfonylurea but not to imidazolinone herbicides.
- R199 (≠ A123) mutation R->A,E: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
- Q207 (= Q131) binding
- K220 (= K144) binding
- R246 (= R170) binding ; binding
- K256 (= K180) binding
- G308 (= G230) binding
- TL 331:332 (= TL 255:256) binding
- C340 (≠ S264) modified: Cysteine sulfinic acid (-SO2H)
- LGMH 349:352 (= LGMH 273:276) binding
- GVRFD 371:375 (≠ GARFD 295:299) binding
- DR 376:377 (= DR 300:301) binding
- DI 395:396 (= DI 320:321) binding
- DV 414:415 (= DV 339:340) binding
- QH 487:488 (= QH 413:414) binding
- GG 508:509 (= GG 434:435) binding
- GAM 511:513 (≠ GTM 437:439) binding
- D538 (≠ E464) binding
- DGS 538:540 (≠ EGS 464:466) binding
- N565 (= N491) binding
- NQHLGM 565:570 (≠ NRYLGM 491:496) binding
- H567 (≠ Y493) binding
- W574 (= W500) binding ; mutation to L: Increased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.; mutation to S: Slightly decreased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.
- S653 (≠ A573) binding ; mutation to A: No effect on catalytic activity or sensitivity to herbicides.; mutation to F: No effect on catalytic activity. Resistant to imidazolinone herbicides and also slightly sulfonylurea-resistant.; mutation to N: In csr1-2/GH90; no effect on catalytic activity. Resistant to imidazolinone but not to sulfonylurea herbicides.; mutation to T: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
5k3sA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, bispyribac-sodium (see paper)
42% identity, 95% coverage: 23:581/587 of query aligns to 14:576/583 of 5k3sA
- active site: Y33 (= Y42), G35 (= G44), G36 (= G45), A37 (≠ S46), S38 (≠ V47), E59 (= E68), T82 (= T91), F121 (= F130), Q122 (= Q131), E123 (= E132), K171 (= K180), M266 (= M275), V293 (= V302), V400 (= V411), G426 (= G437), M428 (= M439), D453 (≠ E464), N480 (= N491), H482 (≠ Y493), L483 (= L494), M485 (= M496), V486 (= V497), W489 (= W500), H558 (≠ P563)
- binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: R292 (= R301), M485 (= M496), W489 (= W500), G569 (= G574)
- binding flavin-adenine dinucleotide: R161 (= R170), G222 (= G229), G223 (= G230), G224 (= G231), T246 (= T255), L247 (= L256), M248 (= M257), L264 (= L273), M266 (= M275), G286 (= G295), R288 (= R297), D290 (= D299), V293 (= V302), D310 (= D320), I311 (= I321), D329 (= D339), V330 (= V340), M405 (= M416), G423 (= G434)
- binding magnesium ion: D453 (≠ E464), N480 (= N491), H482 (≠ Y493)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V411), G401 (= G412), Q402 (= Q413), H403 (= H414), G426 (= G437), M428 (= M439), D453 (≠ E464), G454 (= G465), S455 (= S466), N480 (= N491), H482 (≠ Y493), L483 (= L494), G484 (= G495), M485 (= M496), V486 (= V497)
8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron (see paper)
42% identity, 95% coverage: 23:581/587 of query aligns to 14:576/582 of 8et4A
- binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V411), G401 (= G412), Q402 (= Q413), H403 (= H414), G426 (= G437), M428 (= M439), G452 (= G463), D453 (≠ E464), G454 (= G465), S455 (= S466), M458 (= M469), N480 (= N491), H482 (≠ Y493), L483 (= L494), G484 (= G495), M485 (= M496), V486 (= V497)
- binding flavin-adenine dinucleotide: R161 (= R170), G222 (= G229), G223 (= G230), G224 (= G231), T246 (= T255), L247 (= L256), M248 (= M257), L264 (= L273), M266 (= M275), H267 (= H276), G286 (= G295), V287 (≠ A296), R288 (= R297), D290 (= D299), R292 (= R301), V293 (= V302), D310 (= D320), I311 (= I321), D329 (= D339), V330 (= V340), M405 (= M416), G423 (= G434)
- binding magnesium ion: F370 (= F381), D453 (≠ E464), M458 (= M469), Q461 (= Q472), N480 (= N491), H482 (≠ Y493), K533 (≠ T537)
- binding N-{[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]sulfamoyl}-N-methylmethanesulfonamide: M266 (= M275), R292 (= R301), M485 (= M496), W489 (= W500), S568 (≠ A573)
5wj1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a triazolopyrimidine herbicide, penoxsulam (see paper)
42% identity, 95% coverage: 23:581/587 of query aligns to 14:576/582 of 5wj1A
- active site: Y33 (= Y42), G35 (= G44), G36 (= G45), A37 (≠ S46), S38 (≠ V47), E59 (= E68), T82 (= T91), F121 (= F130), Q122 (= Q131), E123 (= E132), K171 (= K180), M266 (= M275), V293 (= V302), V400 (= V411), G426 (= G437), M428 (= M439), D453 (≠ E464), N480 (= N491), H482 (≠ Y493), L483 (= L494), M485 (= M496), V486 (= V497), W489 (= W500), H558 (≠ P563)
- binding flavin-adenine dinucleotide: R161 (= R170), G222 (= G229), G223 (= G230), G224 (= G231), T246 (= T255), L247 (= L256), M248 (= M257), M263 (= M272), L264 (= L273), G286 (= G295), R288 (= R297), V293 (= V302), D310 (= D320), I311 (= I321), D329 (= D339), V330 (= V340), M405 (= M416), G423 (= G434), G424 (= G435)
- binding magnesium ion: D453 (≠ E464), N480 (= N491), H482 (≠ Y493)
- binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: M266 (= M275), D291 (= D300), R292 (= R301), M485 (= M496), W489 (= W500), S568 (≠ A573)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V411), G401 (= G412), Q402 (= Q413), H403 (= H414), M428 (= M439), D453 (≠ E464), G454 (= G465), S455 (= S466), M458 (= M469), N480 (= N491), H482 (≠ Y493), L483 (= L494), G484 (= G495), M485 (= M496), V486 (= V497)
5k6tA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, propoxycarbazone-sodium (see paper)
42% identity, 95% coverage: 23:581/587 of query aligns to 14:576/582 of 5k6tA
- active site: Y33 (= Y42), G35 (= G44), G36 (= G45), A37 (≠ S46), S38 (≠ V47), E59 (= E68), T82 (= T91), F121 (= F130), Q122 (= Q131), E123 (= E132), K171 (= K180), M266 (= M275), V293 (= V302), V400 (= V411), G426 (= G437), M428 (= M439), D453 (≠ E464), N480 (= N491), H482 (≠ Y493), L483 (= L494), M485 (= M496), V486 (= V497), W489 (= W500), H558 (≠ P563)
- binding methyl 2-[(4-methyl-5-oxidanylidene-3-propoxy-1,2,4-triazol-1-yl)carbonylsulfamoyl]benzoate: H267 (= H276), R292 (= R301), M485 (= M496), W489 (= W500), S568 (≠ A573)
- binding flavin-adenine dinucleotide: R161 (= R170), G222 (= G229), G223 (= G230), G224 (= G231), T246 (= T255), L247 (= L256), M248 (= M257), L264 (= L273), G286 (= G295), R288 (= R297), D290 (= D299), R292 (= R301), V293 (= V302), D310 (= D320), I311 (= I321), D329 (= D339), V330 (= V340), Q404 (= Q415), M405 (= M416), G423 (= G434)
- binding magnesium ion: D453 (≠ E464), N480 (= N491), H482 (≠ Y493)
- binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (= V411), G401 (= G412), Q402 (= Q413), H403 (= H414), G426 (= G437), M428 (= M439), G452 (= G463), G454 (= G465), S455 (= S466), N480 (= N491), H482 (≠ Y493), L483 (= L494), G484 (= G495)
5k6rA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, thiencarbazone-methyl (see paper)
42% identity, 95% coverage: 23:581/587 of query aligns to 14:576/582 of 5k6rA
- active site: Y33 (= Y42), G35 (= G44), G36 (= G45), A37 (≠ S46), S38 (≠ V47), E59 (= E68), T82 (= T91), F121 (= F130), Q122 (= Q131), E123 (= E132), K171 (= K180), M266 (= M275), V293 (= V302), V400 (= V411), G426 (= G437), M428 (= M439), D453 (≠ E464), N480 (= N491), H482 (≠ Y493), L483 (= L494), M485 (= M496), V486 (= V497), W489 (= W500), H558 (≠ P563)
- binding methyl 4-[(3-methoxy-4-methyl-5-oxidanylidene-1,2,4-triazol-1-yl)carbonylsulfamoyl]-5-methyl-thiophene-3-carboxylate: R292 (= R301), W489 (= W500), S568 (≠ A573)
- binding flavin-adenine dinucleotide: R161 (= R170), G222 (= G229), G223 (= G230), G224 (= G231), T246 (= T255), L247 (= L256), M248 (= M257), L264 (= L273), M266 (= M275), G286 (= G295), R288 (= R297), R292 (= R301), V293 (= V302), D310 (= D320), I311 (= I321), G328 (= G338), D329 (= D339), V330 (= V340), M405 (= M416), G423 (= G434)
- binding magnesium ion: D453 (≠ E464), N480 (= N491), H482 (≠ Y493)
- binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (= V411), G401 (= G412), Q402 (= Q413), H403 (= H414), G426 (= G437), M428 (= M439), D453 (≠ E464), G454 (= G465), S455 (= S466), M458 (= M469), N480 (= N491), H482 (≠ Y493), L483 (= L494), G484 (= G495), M485 (= M496), V486 (= V497)
1z8nA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with an imidazolinone herbicide, imazaquin (see paper)
42% identity, 95% coverage: 23:581/587 of query aligns to 14:576/582 of 1z8nA
- active site: Y33 (= Y42), G35 (= G44), G36 (= G45), A37 (≠ S46), S38 (≠ V47), E59 (= E68), T82 (= T91), F121 (= F130), Q122 (= Q131), E123 (= E132), K171 (= K180), M266 (= M275), V293 (= V302), V400 (= V411), G426 (= G437), M428 (= M439), D453 (≠ E464), N480 (= N491), H482 (≠ Y493), L483 (= L494), M485 (= M496), V486 (= V497), W489 (= W500), H558 (≠ P563)
- binding 2-(4-isopropyl-4-methyl-5-oxo-4,5-dihydro-1h-imidazol-2-yl)quinoline-3-carboxylic acid: K135 (= K144), R161 (= R170), Y191 (= Y200), R194 (vs. gap), D291 (= D300), R292 (= R301), D312 (= D322), W489 (= W500), G569 (= G574)
- binding flavin-adenine dinucleotide: R161 (= R170), G222 (= G229), G224 (= G231), T246 (= T255), L247 (= L256), M248 (= M257), L264 (= L273), G265 (= G274), M266 (= M275), H267 (= H276), G286 (= G295), V287 (≠ A296), R288 (= R297), D290 (= D299), R292 (= R301), V293 (= V302), D310 (= D320), I311 (= I321), D329 (= D339), V330 (= V340), M405 (= M416), G423 (= G434), G424 (= G435)
- binding magnesium ion: D453 (≠ E464), N480 (= N491)
- binding thiamine diphosphate: V400 (= V411), G401 (= G412), Q402 (= Q413), H403 (= H414), G426 (= G437), M428 (= M439), G452 (= G463), G454 (= G465), S455 (= S466), N480 (= N491), H482 (≠ Y493), L483 (= L494), G484 (= G495), M485 (= M496), V486 (= V497)
1yi1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, tribenuron methyl (see paper)
42% identity, 95% coverage: 23:581/587 of query aligns to 14:576/582 of 1yi1A
- active site: Y33 (= Y42), G35 (= G44), G36 (= G45), A37 (≠ S46), S38 (≠ V47), E59 (= E68), T82 (= T91), F121 (= F130), Q122 (= Q131), E123 (= E132), K171 (= K180), M266 (= M275), V293 (= V302), V400 (= V411), G426 (= G437), M428 (= M439), D453 (≠ E464), N480 (= N491), H482 (≠ Y493), L483 (= L494), M485 (= M496), V486 (= V497), W489 (= W500), H558 (≠ P563)
- binding methyl 2-[4-methoxy-6-methyl-1,3,5-trazin-2-yl(methyl)carbamoylsulfamoyl]benzoate: D291 (= D300), R292 (= R301), W489 (= W500), S568 (≠ A573)
- binding flavin-adenine dinucleotide: R161 (= R170), G223 (= G230), G224 (= G231), T246 (= T255), L247 (= L256), M248 (= M257), M263 (= M272), L264 (= L273), G265 (= G274), M266 (= M275), H267 (= H276), G286 (= G295), V287 (≠ A296), R288 (= R297), D290 (= D299), V293 (= V302), D310 (= D320), I311 (= I321), D329 (= D339), V330 (= V340), M405 (= M416), G423 (= G434), G424 (= G435)
- binding magnesium ion: D453 (≠ E464), N480 (= N491), H482 (≠ Y493)
1yi0A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, sulfometuron methyl (see paper)
42% identity, 95% coverage: 23:581/587 of query aligns to 14:576/582 of 1yi0A
- active site: Y33 (= Y42), G35 (= G44), G36 (= G45), A37 (≠ S46), S38 (≠ V47), E59 (= E68), T82 (= T91), F121 (= F130), Q122 (= Q131), E123 (= E132), K171 (= K180), M266 (= M275), V293 (= V302), V400 (= V411), G426 (= G437), M428 (= M439), D453 (≠ E464), N480 (= N491), H482 (≠ Y493), L483 (= L494), M485 (= M496), V486 (= V497), W489 (= W500), H558 (≠ P563)
- binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D291 (= D300), R292 (= R301), W489 (= W500), S568 (≠ A573)
- binding flavin-adenine dinucleotide: R161 (= R170), G222 (= G229), G223 (= G230), G224 (= G231), T246 (= T255), L247 (= L256), M248 (= M257), L264 (= L273), G265 (= G274), M266 (= M275), H267 (= H276), G286 (= G295), V287 (≠ A296), R288 (= R297), D290 (= D299), R292 (= R301), V293 (= V302), D310 (= D320), I311 (= I321), G328 (= G338), D329 (= D339), V330 (= V340), M405 (= M416), G423 (= G434), G424 (= G435)
- binding magnesium ion: D453 (≠ E464), N480 (= N491), H482 (≠ Y493)
1yhzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorsulfuron (see paper)
42% identity, 95% coverage: 23:581/587 of query aligns to 14:576/582 of 1yhzA
- active site: Y33 (= Y42), G35 (= G44), G36 (= G45), A37 (≠ S46), S38 (≠ V47), E59 (= E68), T82 (= T91), F121 (= F130), Q122 (= Q131), E123 (= E132), K171 (= K180), M266 (= M275), V293 (= V302), V400 (= V411), G426 (= G437), M428 (= M439), D453 (≠ E464), N480 (= N491), H482 (≠ Y493), L483 (= L494), M485 (= M496), V486 (= V497), W489 (= W500), H558 (≠ P563)
- binding 1-(2-chlorophenylsulfonyl)-3-(4-methoxy-6-methyl-l,3,5-triazin-2-yl)urea: D291 (= D300), R292 (= R301), M485 (= M496), W489 (= W500), S568 (≠ A573)
- binding flavin-adenine dinucleotide: R161 (= R170), G223 (= G230), G224 (= G231), T246 (= T255), L247 (= L256), M248 (= M257), L264 (= L273), M266 (= M275), H267 (= H276), G286 (= G295), V287 (≠ A296), R288 (= R297), D290 (= D299), V293 (= V302), D310 (= D320), I311 (= I321), D329 (= D339), V330 (= V340), Q404 (= Q415), M405 (= M416), G423 (= G434), G424 (= G435)
- binding magnesium ion: D453 (≠ E464), N480 (= N491), H482 (≠ Y493)
1yhyA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl (see paper)
42% identity, 95% coverage: 23:581/587 of query aligns to 14:576/582 of 1yhyA
- active site: Y33 (= Y42), G35 (= G44), G36 (= G45), A37 (≠ S46), S38 (≠ V47), E59 (= E68), T82 (= T91), F121 (= F130), Q122 (= Q131), E123 (= E132), K171 (= K180), M266 (= M275), V293 (= V302), V400 (= V411), G426 (= G437), M428 (= M439), D453 (≠ E464), N480 (= N491), H482 (≠ Y493), L483 (= L494), M485 (= M496), V486 (= V497), W489 (= W500), H558 (≠ P563)
- binding methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D291 (= D300), R292 (= R301), V486 (= V497), W489 (= W500), S568 (≠ A573)
- binding flavin-adenine dinucleotide: R161 (= R170), G222 (= G229), G223 (= G230), G224 (= G231), T246 (= T255), L247 (= L256), M248 (= M257), L264 (= L273), G265 (= G274), M266 (= M275), H267 (= H276), G286 (= G295), V287 (≠ A296), R288 (= R297), D290 (= D299), V293 (= V302), D310 (= D320), I311 (= I321), D329 (= D339), V330 (= V340), Q404 (= Q415), M405 (= M416), G423 (= G434), G424 (= G435)
- binding magnesium ion: D453 (≠ E464), N480 (= N491), H482 (≠ Y493)
1ybhA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide chlorimuron ethyl (see paper)
42% identity, 95% coverage: 23:581/587 of query aligns to 14:576/582 of 1ybhA
- active site: Y33 (= Y42), G35 (= G44), G36 (= G45), A37 (≠ S46), S38 (≠ V47), E59 (= E68), T82 (= T91), F121 (= F130), Q122 (= Q131), E123 (= E132), K171 (= K180), M266 (= M275), V293 (= V302), V400 (= V411), G426 (= G437), M428 (= M439), D453 (≠ E464), N480 (= N491), H482 (≠ Y493), L483 (= L494), M485 (= M496), V486 (= V497), W489 (= W500), H558 (≠ P563)
- binding 2-[[[[(4-chloro-6-methoxy-2-pyrimidinyl)amino]carbonyl]amino]sulfonyl]benzoic acid ethyl ester: M266 (= M275), D291 (= D300), R292 (= R301), M485 (= M496), W489 (= W500), S568 (≠ A573)
- binding flavin-adenine dinucleotide: R161 (= R170), G223 (= G230), G224 (= G231), T246 (= T255), L247 (= L256), M248 (= M257), L264 (= L273), M266 (= M275), H267 (= H276), G286 (= G295), V287 (≠ A296), R288 (= R297), D290 (= D299), V293 (= V302), D310 (= D320), I311 (= I321), D329 (= D339), V330 (= V340), Q404 (= Q415), M405 (= M416), G423 (= G434), G424 (= G435)
- binding magnesium ion: D453 (≠ E464), N480 (= N491), H482 (≠ Y493)
3ea4A Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron-ester (see paper)
42% identity, 95% coverage: 23:581/587 of query aligns to 13:575/582 of 3ea4A
- active site: Y32 (= Y42), G34 (= G44), G35 (= G45), A36 (≠ S46), S37 (≠ V47), E58 (= E68), T81 (= T91), F120 (= F130), Q121 (= Q131), E122 (= E132), K170 (= K180), M265 (= M275), V292 (= V302), V399 (= V411), G425 (= G437), M427 (= M439), D452 (≠ E464), N479 (= N491), H481 (≠ Y493), L482 (= L494), M484 (= M496), V485 (= V497), W488 (= W500), H557 (≠ P563)
- binding methyl 2-{[(4-methylpyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: D290 (= D300), R291 (= R301), W488 (= W500), S567 (≠ A573)
- binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R170), G221 (= G229), G222 (= G230), G223 (= G231), T245 (= T255), L246 (= L256), M247 (= M257), L263 (= L273), G264 (= G274), M265 (= M275), H266 (= H276), G285 (= G295), R287 (= R297), D289 (= D299), R291 (= R301), D309 (= D320), I310 (= I321), G327 (= G338), D328 (= D339), V329 (= V340), M404 (= M416), G422 (= G434)
- binding magnesium ion: D452 (≠ E464), N479 (= N491), H481 (≠ Y493)
- binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (= V411), G400 (= G412), Q401 (= Q413), H402 (= H414), M427 (= M439), G451 (= G463), D452 (≠ E464), G453 (= G465), S454 (= S466), N479 (= N491), H481 (≠ Y493), L482 (= L494), G483 (= G495), M484 (= M496), V485 (= V497)
3e9yA Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron (see paper)
42% identity, 95% coverage: 23:581/587 of query aligns to 13:575/582 of 3e9yA
- active site: Y32 (= Y42), G34 (= G44), G35 (= G45), A36 (≠ S46), S37 (≠ V47), E58 (= E68), T81 (= T91), F120 (= F130), Q121 (= Q131), E122 (= E132), K170 (= K180), M265 (= M275), V292 (= V302), V399 (= V411), G425 (= G437), M427 (= M439), D452 (≠ E464), N479 (= N491), H481 (≠ Y493), L482 (= L494), M484 (= M496), V485 (= V497), W488 (= W500), H557 (≠ P563)
- binding N-[(4-methylpyrimidin-2-yl)carbamoyl]-2-nitrobenzenesulfonamide: D290 (= D300), R291 (= R301), W488 (= W500), S567 (≠ A573)
- binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R170), G221 (= G229), G222 (= G230), G223 (= G231), T245 (= T255), L246 (= L256), M247 (= M257), L263 (= L273), G285 (= G295), R287 (= R297), D289 (= D299), R291 (= R301), D309 (= D320), I310 (= I321), G327 (= G338), D328 (= D339), V329 (= V340), M404 (= M416), G422 (= G434)
- binding magnesium ion: D452 (≠ E464), N479 (= N491), H481 (≠ Y493)
- binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (= V411), G400 (= G412), Q401 (= Q413), H402 (= H414), M427 (= M439), G451 (= G463), G453 (= G465), S454 (= S466), N479 (= N491), H481 (≠ Y493), L482 (= L494), G483 (= G495), M484 (= M496), V485 (= V497)
5k2oA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, pyrithiobac (see paper)
42% identity, 95% coverage: 23:581/587 of query aligns to 14:576/585 of 5k2oA
- active site: Y33 (= Y42), G35 (= G44), G36 (= G45), A37 (≠ S46), S38 (≠ V47), E59 (= E68), T82 (= T91), F121 (= F130), Q122 (= Q131), E123 (= E132), K171 (= K180), M266 (= M275), V293 (= V302), V400 (= V411), G426 (= G437), M428 (= M439), D453 (≠ E464), N480 (= N491), H482 (≠ Y493), L483 (= L494), M485 (= M496), V486 (= V497), W489 (= W500), H558 (≠ P563)
- binding 2-chloranyl-6-(4,6-dimethoxypyrimidin-2-yl)sulfanyl-benzoic acid: M266 (= M275), R292 (= R301), W489 (= W500), S568 (≠ A573)
- binding flavin-adenine dinucleotide: R161 (= R170), G222 (= G229), G223 (= G230), G224 (= G231), T246 (= T255), L247 (= L256), M248 (= M257), L264 (= L273), G286 (= G295), R288 (= R297), D290 (= D299), V293 (= V302), D310 (= D320), I311 (= I321), D329 (= D339), V330 (= V340), Q404 (= Q415), M405 (= M416), G423 (= G434)
- binding magnesium ion: D453 (≠ E464), N480 (= N491), H482 (≠ Y493)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V411), G401 (= G412), Q402 (= Q413), H403 (= H414), M428 (= M439), D453 (≠ E464), G454 (= G465), S455 (= S466), N480 (= N491), H482 (≠ Y493), L483 (= L494), G484 (= G495), M485 (= M496), V486 (= V497)
1t9cA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, sulfometuron methyl (see paper)
43% identity, 95% coverage: 23:581/587 of query aligns to 10:574/596 of 1t9cA
- active site: Y29 (= Y42), G31 (= G44), G32 (= G45), A33 (≠ S46), I34 (≠ V47), E55 (= E68), T78 (= T91), F117 (= F130), Q118 (= Q131), E119 (= E132), K167 (= K180), R227 (= R239), M263 (= M275), V290 (= V302), V406 (= V411), L431 (= L436), G432 (= G437), M434 (= M439), D459 (≠ E464), N486 (= N491), E488 (≠ Y493), Q489 (≠ L494), M491 (= M496), V492 (= V497), W495 (= W500), L517 (= L523), G522 (= G528), L523 (≠ H529), K556 (≠ P563)
- binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: G32 (= G45), V107 (= V120), P108 (= P121), F117 (= F130), K167 (= K180), D288 (= D300), R289 (= R301), W495 (= W500)
- binding flavin-adenine dinucleotide: R157 (= R170), G216 (= G229), A217 (≠ G230), G218 (= G231), N221 (vs. gap), T243 (= T255), L244 (= L256), Q245 (≠ M257), L261 (= L273), M263 (= M275), H264 (= H276), G283 (= G295), A284 (= A296), R285 (= R297), D287 (= D299), R289 (= R301), V290 (= V302), E316 (≠ D320), V317 (≠ I321), N321 (≠ S325), G334 (= G338), D335 (= D339), A336 (≠ V340), M411 (= M416), G429 (= G434), G430 (= G435)
- binding magnesium ion: D459 (≠ E464), N486 (= N491), E488 (≠ Y493)
P07342 Acetolactate synthase catalytic subunit, mitochondrial; Acetohydroxy-acid synthase catalytic subunit; AHAS; ALS; EC 2.2.1.6 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
42% identity, 95% coverage: 23:581/587 of query aligns to 94:665/687 of P07342
- R241 (= R170) binding
- 355:376 (vs. 276:297, 55% identical) binding
- 407:426 (vs. 320:339, 35% identical) binding
1t9dA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl (see paper)
42% identity, 95% coverage: 23:581/587 of query aligns to 10:574/596 of 1t9dA
- active site: Y29 (= Y42), G31 (= G44), G32 (= G45), A33 (≠ S46), I34 (≠ V47), E55 (= E68), T78 (= T91), F117 (= F130), Q118 (= Q131), E119 (= E132), K167 (= K180), R227 (= R239), M263 (= M275), V290 (= V302), V406 (= V411), L431 (= L436), G432 (= G437), M434 (= M439), D459 (≠ E464), N486 (= N491), E488 (≠ Y493), Q489 (≠ L494), M491 (= M496), V492 (= V497), W495 (= W500), L517 (= L523), G522 (= G528), L523 (≠ H529), K556 (≠ P563)
- binding methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: G32 (= G45), A33 (≠ S46), V107 (= V120), P108 (= P121), F117 (= F130), K167 (= K180), M263 (= M275), D288 (= D300), R289 (= R301), W495 (= W500)
- binding flavin-adenine dinucleotide: R157 (= R170), G216 (= G229), A217 (≠ G230), G218 (= G231), N221 (vs. gap), T243 (= T255), L244 (= L256), Q245 (≠ M257), M260 (= M272), L261 (= L273), H264 (= H276), G283 (= G295), A284 (= A296), R285 (= R297), D287 (= D299), R289 (= R301), V290 (= V302), E316 (≠ D320), V317 (≠ I321), N321 (≠ S325), G334 (= G338), D335 (= D339), A336 (≠ V340), Q410 (= Q415), M411 (= M416), G429 (= G434), G430 (= G435)
- binding magnesium ion: D459 (≠ E464), N486 (= N491), E488 (≠ Y493)
- binding 2,5-dimethyl-pyrimidin-4-ylamine: E55 (= E68), P81 (= P94), Q118 (= Q131), G432 (= G437), M434 (= M439), M464 (= M469)
Query Sequence
>WP_013088923.1 NCBI__GCF_000092885.1:WP_013088923.1
MNMPSAEFSTSDTTPPHEADSIGATVLMKALADEDVEFIWGYPGGSVLYIYDELYKQDKF
QHVLVRHEQAAVHAADAYARSTGKVGVCLVTSGPGVTNAVTGIATAYMDSIPMVVISGQV
PTAAIGQDAFQECDTVGITRPCVKHNFLVKDVRDLAATVKKAFYIARTGRPGPVLIDIPK
DVSKAPCQYEPIKSVSLRSYNPVTKGHSGQIRKAVSLLLSAKRPYIYTGGGIVLADASRE
LNQFADLLGYPVTNTLMGLGGYRSSDKKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDD
RVIGDPNHFASRPRKIIHIDIDPSSISKRVKVDIPIVGDVKEVLKELIEQLQTAEHGPDT
AALADWWKDIEGWRAKDCLKFDRKSDIIKPQYVVEKAWELTDGNAFVCSDVGQHQMWAAQ
FYKFNKPRHWINSGGLGTMGFGLPAAMGVKMAHPDDDVLCITGEGSIQMCIQELSTCKQY
DLPIKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAEAYGHVGMRIERTADV
EPALKEALRLKDRTVFLDFQTDPTENVWPMVQAGKGITEMLMGSEDL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory