SitesBLAST
Comparing WP_013089220.1 NCBI__GCF_000092885.1:WP_013089220.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
O59010 Glutamate transporter homolog; Glt(Ph); Sodium-aspartate symporter Glt(Ph); Sodium-dependent aspartate transporter from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 3 papers)
28% identity, 93% coverage: 7:412/435 of query aligns to 13:425/425 of O59010
- S65 (≠ T57) mutation to V: Strongly decreased chloride conductance.
- R276 (≠ S268) mutation to S: Increased rate of aspartate transport; when associated with R-395.
- RSS 276:278 (≠ SST 268:270) binding L-aspartate
- M311 (≠ L303) mutation to A: Decreased dependence of aspartate binding on Na(+) concentration.
- T314 (= T306) binding L-aspartate
- V355 (= V347) binding L-aspartate
- D394 (≠ S381) binding L-aspartate
- M395 (≠ E382) mutation to R: Increased rate of aspartate transport; when associated with S-276.
- R397 (= R384) mutation to A: Strongly decreased affinity for aspartate.
- N401 (≠ S388) binding L-aspartate
- D405 (≠ N392) mutation to N: Strongly decreased affinity for aspartate.
6x15A Inward-facing state of the glutamate transporter homologue gltph in complex with l-aspartate and sodium ions (see paper)
29% identity, 91% coverage: 7:404/435 of query aligns to 13:417/419 of 6x15A
- binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: F46 (≠ L38), F46 (≠ L38), P75 (≠ S67), L91 (≠ C84), F95 (≠ V88), L130 (= L130), I133 (≠ F133), I159 (≠ L159), Y167 (≠ F163), K196 (≠ R188), G200 (≠ M192), I207 (≠ L199), F210 (= F202), L250 (= L242), I262 (≠ L254), M269 (≠ L261), T334 (≠ S326), V335 (≠ A327), G336 (≠ S328), T340 (= T332), L343 (≠ A335), M399 (≠ L386)
- binding aspartic acid: S277 (= S269), S278 (≠ T270), T314 (= T306), G354 (= G346), A358 (≠ S350), G359 (= G351), D394 (≠ S381), R397 (= R384), T398 (≠ A385)
- binding sodium ion: Y89 (≠ F82), T92 (≠ L85), S93 (≠ T86), G306 (= G298), T308 (≠ S300), N310 (= N302), N310 (= N302), M311 (≠ L303), D312 (= D304), S349 (= S341), I350 (≠ K342), T352 (≠ A344), N401 (≠ S388), V402 (= V389), D405 (≠ N392)
Sites not aligning to the query:
2nwwA Crystal structure of gltph in complex with tboa (see paper)
28% identity, 91% coverage: 7:403/435 of query aligns to 4:407/407 of 2nwwA
6x14A Inward-facing state of the glutamate transporter homologue gltph in complex with tfb-tboa (see paper)
29% identity, 91% coverage: 7:403/435 of query aligns to 10:413/413 of 6x14A
- binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: G66 (= G61), V83 (≠ L79), I157 (≠ L160), Y164 (≠ F163), K193 (≠ R188), T305 (≠ S300), I306 (≠ F301), I347 (≠ K342)
- binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I13 (≠ L10), M199 (= M194), S275 (≠ T270), T311 (= T306), G356 (= G351), L384 (≠ V374), D391 (≠ S381), R394 (= R384)
Sites not aligning to the query:
6bavA Crystal structure of gltph r397c in complex with s-benzyl-l-cysteine (see paper)
28% identity, 91% coverage: 7:404/435 of query aligns to 5:409/409 of 6bavA
6bauA Crystal structure of gltph r397c in complex with l-cysteine (see paper)
28% identity, 91% coverage: 7:403/435 of query aligns to 5:408/408 of 6bauA
- binding cysteine: S270 (≠ T270), M303 (≠ L303), T306 (= T306), A345 (= A345), G346 (= G346), V347 (= V347), G351 (= G351), D386 (≠ S381), C389 (≠ R384), T390 (≠ A385), N393 (≠ S388)
6zgbA Glutamate transporter homologue glttk in complex with a photo cage compound (see paper)
27% identity, 91% coverage: 15:410/435 of query aligns to 17:421/425 of 6zgbA
6zl4A The structure of glutamate transporter homologue glttk in complex with the photo switchable compound (cis) (see paper)
27% identity, 91% coverage: 15:410/435 of query aligns to 16:420/424 of 6zl4A
- binding decyl-beta-d-maltopyranoside: L191 (≠ R188), G195 (≠ M192), R282 (≠ G279)
- binding (2~{S},3~{S})-2-azanyl-3-[[4-[2-(4-methoxyphenyl)hydrazinyl]phenyl]methoxy]butanedioic acid: R271 (≠ S268), S272 (= S269), S273 (≠ T270), M307 (≠ L303), T310 (= T306), G353 (= G349), A354 (≠ S350), R394 (= R384), T395 (≠ A385)
Sites not aligning to the query:
6xwnB Structure of glutamate transporter homologue glttk in the presence of tboa inhibitor (see paper)
27% identity, 91% coverage: 15:410/435 of query aligns to 19:423/426 of 6xwnB
5e9sA Crystal structure of substrate-bound glutamate transporter homologue glttk (see paper)
27% identity, 91% coverage: 15:410/435 of query aligns to 19:423/427 of 5e9sA
- binding aspartic acid: R274 (≠ S268), S275 (= S269), S276 (≠ T270), T313 (= T306), G353 (= G346), V354 (= V347), A357 (≠ S350), G358 (= G351), D394 (≠ S381), R397 (= R384), T398 (≠ A385)
- binding decyl-beta-d-maltopyranoside: L194 (≠ R188), G198 (≠ M192), Y202 (≠ L196)
- binding sodium ion: Y87 (≠ F82), T90 (≠ L85), S91 (≠ T86), S276 (≠ T270), G305 (= G298), A306 (≠ Y299), T307 (≠ S300), N309 (= N302), N309 (= N302), M310 (≠ L303), D311 (= D304), S348 (= S341), I349 (≠ K342), G350 (= G343), T351 (≠ A344), N401 (≠ S388), V402 (= V389), D405 (≠ N392)
6r7rA Crystal structure of the glutamate transporter homologue glttk in complex with d-aspartate (see paper)
27% identity, 91% coverage: 15:410/435 of query aligns to 12:412/416 of 6r7rA