SitesBLAST
Comparing WP_013089850.1 NCBI__GCF_000092885.1:WP_013089850.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 6 hits to proteins with known functional sites (download)
P25297 Inorganic phosphate transporter PHO84 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 6 papers)
25% identity, 74% coverage: 89:451/489 of query aligns to 117:550/587 of P25297
- C145 (≠ G117) mutation to S: No significant effect on transport activity; when associated with S-237, S-241, S-245, S-263, S-335, S-399, S-434, S-455, S-474, S-510 and S-519.
- F160 (≠ I132) mutation to C: Reduces phosphate binding, transport and signaling activities.
- R168 (= R142) mutation to A: Reduces transport activity. No significant effects on affinity for inorganic phosphate. No significant effects on growth rates.; mutation to C: Abolishes transport and signaling activities.; mutation to E: Reduces transport activity. Moderately reduces affinity for inorganic phosphate. No significant effects on growth rates.; mutation to Q: Reduces transport activity. Moderately reduces affinity for inorganic phosphate. Moderately reduces growth rates under low phosphate conditions. No significant effects on growth rates under high phosphate conditions.
- G172 (= G146) mutation to C: Abolishes transport and signaling activities.
- G174 (≠ S148) mutation to C: Abolishes transport and signaling activities.
- G176 (= G150) mutation to C: Abolishes transport and signaling activities.
- D178 (≠ E152) mutation D->E,N: Reduces transport activity. No significant effects on affinity for inorganic phosphate. No significant effects on signaling activities. No significant effects on growth rates.
- Y179 (= Y153) mutation to A: Significantly reduces transport activity. Causes pronounced increase in secreted phosphatase activity under high phosphate conditions. Reduces growth rates under low phosphate conditions. Slightly reduces signaling activity.; mutation to G: Abolishes transport activity. Causes pronounced increase in secreted phosphatase activity under high phosphate conditions. Reduces growth rates under low phosphate conditions. Abolishes signaling activity.; mutation Y->S,F: No significant effects on transport activity. No significant effects on growth rates under low phosphate conditions. Does not affect patterns of secreted phosphatase activities under low and high phosphate conditions. No significant effects on signaling activity.
- C237 (vs. gap) mutation to S: No significant effect on transport activity; when associated with S-145, S-241, S-245, S-263, S-335, S-399, S-434, S-455, S-474, S-510 and S-519.
- C241 (≠ T200) mutation to S: No significant effect on transport activity; when associated with S-145, S-237, S-245, S-263, S-335, S-399, S-434, S-455, S-474, S-510 and S-519.
- C245 (≠ L204) mutation to S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-263, S-335, S-399, S-434, S-455, S-474, S-510 and S-519.
- C263 (≠ G222) mutation to S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-335, S-399, S-434, S-455, S-474, S-510 and S-519.
- K298 (≠ Q238) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); mutation to A: No significant effect on membrane localization under low phosphate conditions and internalization after phosphate addition. Strongly affects vacuolar sorting of the protein after internalization.
- 304:327 (vs. 244:250, 17% identical) mutation Missing: Increases transport activity. Has no significant effect on membrane localization under low phosphate conditions. Results in severely delayed internalization after phosphate addition.
- C335 (≠ L258) mutation to S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-263, S-399, S-434, S-455, S-474, S-510 and S-519.
- D358 (≠ N276) mutation to E: Abolishes transport activity. Causes pronounced increase in secreted phosphatase activity under high phosphate conditions. Moderately decreases signaling activities. Significantly reduces growth rates under low phosphate conditions. No significant effects on growth rates under high phosphate conditions.; mutation to N: Significantly reduces transport activity. No significant effects on affinity for inorganic phosphate. Causes pronounced increase in secreted phosphatase activity under high phosphate conditions. No significant effects on signaling activities. Significantly reduces growth rates under low phosphate conditions. No significant effects on growth rates under high phosphate conditions.
- V392 (≠ L311) mutation to C: Reduces transport and signaling activities.
- C399 (≠ A318) mutation to S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-263, S-335, S-434, S-455, S-474, S-510 and S-519.
- V408 (vs. gap) mutation to C: Reduces signaling activity and enhances transport activity.
- C434 (≠ S343) mutation to S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-263, S-335, S-399, S-455, S-474, S-510 and S-519.
- C455 (≠ L369) mutation to S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-263, S-335, S-399, S-434, S-474, S-510 and S-519.
- E473 (≠ A382) mutation to K: No significant effects on transport activity. No significant effects on affinity for inorganic phosphate. No significant effects on growth rates.; mutation to Q: No significant effects on transport activity. Reduces affinity for inorganic phosphate. No significant effects on growth rates.
- C474 (≠ L383) mutation to S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-263, S-335, S-399, S-434, S-455, S-510 and S-519.
- K492 (≠ S400) mutation K->A,Q: No significant effects on transport activity. Reduces affinity for inorganic phosphate. No significant effects on growth rates.; mutation to E: Reduces transport activity. Reduces affinity for inorganic phosphate. Significantly reduces growth rates under low phosphate conditions. No significant effects on growth rates under high phosphate conditions.
- C510 (vs. gap) mutation to S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-263, S-335, S-399, S-434, S-455, S-474 and S-519.
- C519 (≠ L422) mutation to S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-263, S-335, S-399, S-434, S-455, S-474 and S-510.
Sites not aligning to the query:
- 6 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
Q8NLB7 Gentisate transporter from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534) (see paper)
26% identity, 53% coverage: 47:306/489 of query aligns to 56:294/444 of Q8NLB7
- D57 (= D48) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
- R103 (= R100) mutation to A: Loss of transport activity.
Sites not aligning to the query:
- 54 D→A: Loss of transport activity.; D→E: Retains 50% of its transport activity.
- 309 W→V: Loss of transport activity.
- 312 D→A: Loss of transport activity.
- 313 R→A: Loss of transport activity.
- 317 mutation I->H,Y: Loss of transport activity.
- 386 R→A: Loss of transport activity.
A0A0H2VG78 Glucose transporter GlcP; Glucose/H(+) symporter from Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200) (see paper)
24% identity, 62% coverage: 95:395/489 of query aligns to 63:375/446 of A0A0H2VG78
- R102 (= R142) mutation to A: Loss of transport activity.
- I105 (≠ Q145) mutation to S: Affects symport activity. May function as an uniporter.
- E122 (= E162) mutation to A: Loss of transport activity.
- Q137 (≠ E177) mutation to A: Loss of transport activity.
- Q250 (vs. gap) mutation to A: Loss of transport activity.
- Q251 (≠ N276) mutation to A: Loss of transport activity.
- N256 (≠ M281) mutation to A: Loss of transport activity.
- W357 (≠ P377) mutation to A: Loss of transport activity.
Sites not aligning to the query:
- 22 D→N: Affects symport activity. May function as an uniporter.
Q9Y7Q9 Probable metabolite transporter C2H8.02 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
27% identity, 33% coverage: 81:242/489 of query aligns to 90:258/583 of Q9Y7Q9
Sites not aligning to the query:
- 267 modified: Phosphoserine
- 269 modified: Phosphoserine
- 289 modified: Phosphoserine
- 290 modified: Phosphoserine
- 292 modified: Phosphoserine
- 330 modified: Phosphoserine
6rw3A The molecular basis for sugar import in malaria parasites. (see paper)
23% identity, 52% coverage: 138:392/489 of query aligns to 99:381/437 of 6rw3A
8ttgA Nora single mutant - e222q at ph 7.5 (see paper)
24% identity, 32% coverage: 78:233/489 of query aligns to 41:180/374 of 8ttgA
Sites not aligning to the query:
Query Sequence
>WP_013089850.1 NCBI__GCF_000092885.1:WP_013089850.1
MTASSNGFWRHHKQEQRLSLDDITVVDHSLLKRAVGAMALGNAMEWFDFGVYSYIAVTLG
KVFFPSSSPSAQLIATFGTFAAAFLVRPIGGTVFGPLGDRIGRQRVLAMTMIMMAAGTFA
IGLIPNYATIGILAPVLLLLARLVQGFSTGGEYGGAATFIAEFSTDKRRGFMGSFLEFGT
LIGYVLGAGTVALLTATLSTDELLSWGWRVPFLIAGPLGLVGLYIRMKLEETPAFKKQAE
QREAEDRAVPKQSFRQLLLQQWRPLLLCVGLVLIFNVTDYMALSYLPSYLSATLHFNETH
GLFLVLLVMILMMPLTLAAGRLSDAIGRKPVMLLGCVGLFALSIPALLLIRMGTVLPVFA
GLMILGVLLSCFTGVMPSALPALFPTRIRYGALAIGFNISVSLFGGTTPLVTAWLVDRTG
NLMMPAYYLMGASLIGIVSVLALRETARKPLLGSGPCVATRAEAHAVLRGEREAEEMDEG
FAAGATARA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory