SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013092160.1 NCBI__GCF_000092885.1:WP_013092160.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9X5C9 Quinate/shikimate dehydrogenase (NAD(+)); QSDH; EC 1.1.1.-; EC 1.1.1.24 from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534) (see paper)
51% identity, 89% coverage: 33:309/312 of query aligns to 4:283/283 of Q9X5C9

query
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Q9X5C9

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S17 (= S46) binding shikimate
SRT 17:19 (≠ SLT 46:48) binding L-quinate
T69 (= T98) binding L-quinate; binding shikimate
K73 (= K102) active site, Proton acceptor; binding L-quinate; binding shikimate
N94 (= N123) binding L-quinate; binding shikimate
D110 (= D138) binding L-quinate; binding shikimate
GV 137:138 (≠ GA 165:166) binding NAD(+)
D158 (= D186) binding NAD(+)
R163 (= R191) binding NAD(+)
PMGM 203:206 (≠ PTGM 229:232) binding NAD(+)
A213 (≠ P239) binding NAD(+)
V228 (≠ I254) binding NAD(+)
G251 (= G277) binding NAD(+)
Q258 (= Q284) binding L-quinate; binding shikimate

3jyqA Quinate dehydrogenase from corynebacterium glutamicum in complex with shikimate and nadh (see paper)
51% identity, 89% coverage: 33:309/312 of query aligns to 3:282/282 of 3jyqA

query
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3jyqA

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binding nicotinamide-adenine-dinucleotide: V132 (≠ L161), G135 (= G164), G136 (= G165), V137 (≠ A166), D157 (= D186), L158 (≠ V187), R162 (= R191), T201 (= T228), P202 (= P229), M205 (= M232), V227 (≠ I254), A254 (= A281)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S16 (= S46), N66 (= N96), T68 (= T98), N93 (= N123), D109 (= D138), Q257 (= Q284)

3jypA Quinate dehydrogenase from corynebacterium glutamicum in complex with quinate and nadh (see paper)
51% identity, 89% coverage: 33:309/312 of query aligns to 3:282/282 of 3jypA

query
sites
3jypA

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binding nicotinamide-adenine-dinucleotide: V132 (≠ L161), G135 (= G164), V137 (≠ A166), D157 (= D186), L158 (≠ V187), R162 (= R191), T201 (= T228), P202 (= P229), M205 (= M232), A212 (≠ P239), V227 (≠ I254), Y229 (= Y256), A254 (= A281)
binding (1S,3R,4S,5R)-1,3,4,5-tetrahydroxycyclohexanecarboxylic acid: S16 (= S46), T18 (= T48), N66 (= N96), T68 (= T98), K72 (= K102), N93 (= N123), D109 (= D138), Q257 (= Q284)

3jyoA Quinate dehydrogenase from corynebacterium glutamicum in complex with NAD (see paper)
51% identity, 89% coverage: 33:309/312 of query aligns to 3:282/282 of 3jyoA

query
sites
3jyoA

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binding nicotinamide-adenine-dinucleotide: V132 (≠ L161), G135 (= G164), V137 (≠ A166), D157 (= D186), L158 (≠ V187), R162 (= R191), T201 (= T228), P202 (= P229), M205 (= M232), V227 (≠ I254), Y229 (= Y256), A254 (= A281)

2hk9B Crystal structure of shikimate dehydrogenase from aquifex aeolicus in complex with shikimate and NADP+ at 2.2 angstrom resolution (see paper)
32% identity, 85% coverage: 32:296/312 of query aligns to 5:254/267 of 2hk9B

query
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2hk9B

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P

Q

E

K

E

L

L

D

K

T

K

A

A

A

-

L

-

P

-

E

-

L

-

L

F

T

E

A

G

A

F

E

K

R

A

M

L

G

K

Y

V

N

K

G

G

L

I

N
|
N

I

V

T
|
T

Y
x
V

P

P

C

F

K
|
K

Q

E

A

E

V

I

I

I

P

P

L

L

D

D

E

Y

L

V

S

E

D

D

D

T

A

A

R

K

A

E

L

I

G

G

A

A

V

V

N
|
N

T

T

V

V

L

K

F

F

K

E

D

N

G

G

K

K

R

A

I

Y

G

G

H

Y

N

N

T

T

D
|
D

W

W

S

I

G

G

F

F

A

L

R

K

A

S

F

L

Q

K

R

S

G

L

L

I

P

P

D

E

V

V

S

K

L

E

E

K

R

S

V

I

V

L

Q

V

L

L

G
|
G

A

A

G

G

G
|
G

A
|
A

G

S

A

R

A

A

V

V

A

I

H

Y

A

A

A

L

L

V

N

K

M

E

G

G

A

A

Q

K

S

V

L

F

T

-

L

L

F

W

D
x
N

V

-

D

-

A

-

R
|
R

A
x
T

Q

K

S

E

L
x
K

A

A

D

I

E

K

L

L

Q

A

A

Q

R

K

F

F

P

P

K

-

A

-

K

-

V

L

S

E

A

V

G

V

T

N

S

S

L

P

E

E

S

V

L

I

A

D

A

K

A

V

Q

Q

G

V

L

I

I

V

H

N

A

T

T
|
T

P
x
S

T
x
V

G

G

M

L

A

K

K

D

M

E

P

D

G

P

L

E

P

I

L

F

P

N

A

Y

E

D

L

L

L

I

H

K

R

K

D

D

L

H

W

V

V

V

A

V

D

D

I
|
I

V

I

Y
|
Y

F

-

P

-

I

K

R

E

T

T

A

K

L

L

Q

K

A

K

A

A

E

K

A

E

L

K

G

G

C

A

R

K

T

L

L

L

S

D

G

G

G

L

G

P

M
|
M

A
x
L

V

L

Y

W

Q
|
Q

A

G

V

I

D

E

A

A

M

F

R

K

I

I

F

W

T

N

G

G

L

C

E

E

binding nadp nicotinamide-adenine-dinucleotide phosphate: V67 (≠ Y99), G130 (= G162), G133 (= G165), A134 (= A166), N153 (≠ D186), R154 (= R191), T155 (≠ A192), K158 (≠ L195), T188 (= T228), S189 (≠ P229), V190 (≠ T230), I214 (= I254), M238 (= M280), L239 (≠ A281)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S19 (= S46), S21 (≠ T48), N64 (= N96), T66 (= T98), K70 (= K102), N91 (= N123), D106 (= D138), Y216 (= Y256), L239 (≠ A281), Q242 (= Q284)

2hk9A Crystal structure of shikimate dehydrogenase from aquifex aeolicus in complex with shikimate and NADP+ at 2.2 angstrom resolution (see paper)
32% identity, 85% coverage: 32:296/312 of query aligns to 5:254/269 of 2hk9A

query
sites
2hk9A

Q

Q

S

T

F

Q

L

L

V

Y

G

G

L

V

I

I

G

G

S

F

G

P

I

V

S

K

G

H

S
|
S

L

L

T
x
S

P

P

A

V

M

F

H

Q

E

N

E

A

E

L

G

I

S

R

R

Y

L

A

G

G

L

L

H

N

Y

A

V

V

Y

Y

R

L

R

A

I

F

D

E

L

I

D

N

A

P

Q

E

K

E

L

L

D

K

T

K

A

A

A

-

L

-

P

-

E

-

L

-

L

F

T

E

A

G

A

F

E

K

R

A

M

L

G

K

Y

V

N

K

G

G

L

I

N
|
N

I

V

T

T

Y
x
V

P

P

C

F

K
|
K

Q

E

A

E

V

I

I

I

P

P

L

L

D

D

E

Y

L

V

S

E

D

D

D

T

A

A

R

K

A

E

L

I

G

G

A

A

V

V

N
|
N

T

T

V

V

L

K

F

F

K

E

D

N

G

G

K

K

R

A

I

Y

G

G

H

Y

N

N

T

T

D
|
D

W

W

S

I

G

G

F

F

A

L

R

K

A

S

F

L

Q

K

R

S

G

L

L

I

P

P

D

E

V

V

S

K

L

E

E

K

R

S

V

I

V

L

Q

V

L

L

G

G

A

A

G
|
G

A
|
A

G

S

A

R

A

A

V

V

A

I

H

Y

A

A

A

L

L

V

N

K

M

E

G

G

A

A

Q

K

S

V

L

F

T

-

L

L

F

W

D
x
N

V

-

D

-

A

-

R
|
R

A
x
T

Q

K

S

E

L

K

A

A

D

I

E

K

L

L

Q

A

A

Q

R

K

F

F

P

P

K

-

A

-

K

-

V

L

S

E

A

V

G

V

T

N

S

S

L

P

E

E

S

V

L

I

A

D

A

K

A

V

Q

Q

G

V

L

I

I

V

H

N

A

T

T
|
T

P
x
S

T
x
V

G

G

M

L

A

K

K

D

M

E

P

D

G

P

L

E

P

I

L

F

P

N

A

Y

E

D

L

L

L

I

H

K

R

K

D

D

L

H

W

V

V

V

A

V

D

D

I

I

V

I

Y
|
Y

F

-

P

-

I

K

R

E

T

T

A

K

L

L

Q

K

A

K

A

A

E

K

A

E

L

K

G

G

C

A

R

K

T

L

L

L

S

D

G

G

G

L

G

P

M

M

A
x
L

V

L

Y

W

Q
|
Q

A

G

V

I

D

E

A

A

M

F

R

K

I

I

F

W

T

N

G

G

L

C

E

E

binding 2'-monophosphoadenosine-5'-diphosphate: V67 (≠ Y99), G132 (= G164), G133 (= G165), A134 (= A166), N153 (≠ D186), R154 (= R191), T155 (≠ A192), T188 (= T228), S189 (≠ P229), V190 (≠ T230)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S19 (= S46), S21 (≠ T48), N64 (= N96), K70 (= K102), N91 (= N123), D106 (= D138), Y216 (= Y256), L239 (≠ A281), Q242 (= Q284)

O67049 Shikimate dehydrogenase (NADP(+)); SD; SDH; EC 1.1.1.25 from Aquifex aeolicus (strain VF5) (see paper)
32% identity, 85% coverage: 32:296/312 of query aligns to 5:254/269 of O67049

query
sites
O67049

Q

Q

S

T

F

Q

L

L

V

Y

G

G

L

V

I

I

G

G

S

F

G

P

I

V

S

K

G

H

S
|
S

L
|
L

T
x
S

P

P

A

V

M

F

H

Q

E

N

E

A

E

L

G

I

S

R

R

Y

L

A

G

G

L

L

H

N

Y

A

V

V

Y

Y

R

L

R

A

I

F

D

E

L

I

D

N

A

P

Q

E

K

E

L

L

D

K

T

K

A

A

A

-

L

-

P

-

E

-

L

-

L

F

T

E

A

G

A

F

E

K

R

A

M

L

G

K

Y

V

N

K

G

G

L

I

N

N

I

V

T

T

Y

V

P

P

C

F

K

K

Q

E

A

E

V

I

I

I

P

P

L

L

D

D

E

Y

L

V

S

E

D
|
D

D

T

A

A

R

K

A

E

L

I

G

G

A

A

V

V

N
|
N

T

T

V

V

L

K

F

F

K

E

D

N

G

G

K

K

R

A

I

Y

G

G

H

Y

N

N

T

T

D
|
D

W

W

S

I

G

G

F

F

A

L

R

K

A

S

F

L

Q

K

R

S

G

L

L

I

P

P

D

E

V

V

S

K

L

E

E

K

R

S

V

I

V

L

Q

V

L

L

G
|
G

A
|
A

G
|
G

A
|
A

G

S

A

R

A

A

V

V

A

I

H

Y

A

A

A

L

L

V

N

K

M

E

G

G

A

A

Q

K

S

V

L

F

T

L

L

W

F

-

D

-

V

-

D

-

A

-

A

N

R

R

A

T

Q

K

S

E

L

K

A

A

D

I

E

K

L

L

Q

A

A

Q

R

K

F

F

P

P

K

-

A

-

K

-

V

L

S

E

A

V

G

V

T

N

S

S

L

P

E

E

S

V

L

I

A

D

A

K

A

V

Q

Q

G

V

L

I

I

V

H

N

A

T

T

T

P

S

T

V

G

G

M

L

A

K

K

D

M

K

P

D

G

P

L

E

P

I

L

F

P

N

A

Y

E

D

L

L

L

I

H

K

R

K

D

D

L

H

W

V

V

V

A

V

D

D

I
|
I

V

I

Y
|
Y

F

-

P

-

I

K

R

E

T

T

A

K

L

L

Q

K

A

K

A

A

E

K

A

E

L

K

G

G

C

A

R

K

T

L

L

F

S

D

G
|
G

G

L

G

P

M

M

A

L

V

L

Y

W

Q
|
Q

A

G

V

I

D

E

A

A

M

F

R

K

I

I

F

W

T

N

G

G

L

C

E

E

SLS 19:21 (≠ SLT 46:48) binding shikimate
D82 (= D114) binding NADP(+)
N91 (= N123) binding shikimate
D106 (= D138) binding shikimate
GAGGA 130:134 (= GAGGA 162:166) binding NADP(+)
I214 (= I254) binding NADP(+)
Y216 (= Y256) binding shikimate
G235 (= G277) binding NADP(+)
Q242 (= Q284) binding shikimate

3tozA 2.2 angstrom crystal structure of shikimate 5-dehydrogenase from listeria monocytogenes in complex with NAD.
33% identity, 86% coverage: 28:296/312 of query aligns to 8:280/291 of 3tozA

query
sites
3tozA

R

R

A

I

T

T

P

G

Q

H

S

T

F

E

L

L

V

I

G

G

L

L

I

I

G

A

S

T

G

P

I

I

S

R

G

H

S

S

L

L

T

S

P

P

A

T

M

M

H

H

E

N

E

E

E

A

G

F

S

A

R

K

L

L

G

G

L

L

H

D

Y

Y

V

V

Y

Y

R

L

R

A

I

F

D

E

L

V

D

G

A

D

Q

K

K

E

L

L

D

-

T

-

A

-

L

-

P

K

E

D

L

V

T

Q

A

G

A

F

E

R

R

A

M

M

G

N

Y

L

N

R

G

G

L

W

N

N

I

V

T

S

Y

M

P

P

C

N

K

K

Q

T

A

N

V

I

I

H

P

K

L

Y

L

L

D

D

E

K

L

L

S

S

D

P

D

A

A

A

R

E

A

L

L

V

G

G

A

A

V

V

N

N

T

T

V

V

L

V

F

N

K

D

D

D

G

G

K

V

R

L

I

T

G

G

H

H

N

I

T

T

D

D

W

G

S

T

G

G

F

Y

A

M

R

R

A

A

F

L

Q

K

R

E

G

A

L

G

P

H

D

D

V

I

S

I

L

G

E

K

R

K

V

M

V

T

Q

I

L

C

G
|
G

A
|
A

G
|
G

A
|
A

G

A

A

T

A

A

V

I

A

C

H

I

A

Q

A

A

L

A

N

L

M

D

G

G

A

V

Q

K

S

E

L

I

T

S

L

I

F

F

-
x
N

-
x
R

-

K

D
|
D

V

D

D
x
F

A

Y

A

A

R

N

A

A

Q

E

S

K

L

T

A

V

D

E

E

K

L

I

Q

N

A

S

R

K

F

T

P

D

-

C

K

K

A

A

K

Q

-

L

-

F

-

D

V

I

S

E

A

D

G

H

T

E

S

Q

L

L

E

R

E

K

S

E

L

I

A

A

A

E

A

S

Q

V

G

I

L

F

I

T

H

N

A

A

T
|
T

P
x
G

T
x
V

G

G

M
|
M

A

K

K

P

M
x
F

P

E

G

G

L

E

P

T

L

L

-

L

-

P

P

S

A

A

E

D

L

M

L

L

H

R

R

P

D

E

L

L

W

I

V

V

A

S

D

D

I
x
V

V

V

Y
|
Y

F

K

P

P

I

T

R

K

T

T

A

R

L

L

Q

E

A

I

A

A

E

E

A

E

L

Q

G

G

C

C

R

Q

T

T

L

L

S

N

G
|
G

G

L

G

G

M
|
M

A
x
M

V

L

Y

W

Q

Q

A

G

V

A

D

K

A

A

M

F

R

E

I

I

F

W

T

T

G

H

L

K

E

E

binding nicotinamide-adenine-dinucleotide: G137 (= G162), A138 (= A163), G139 (= G164), G140 (= G165), A141 (= A166), N161 (vs. gap), R162 (vs. gap), D164 (= D186), F166 (≠ D188), T210 (= T228), G211 (≠ P229), V212 (≠ T230), M214 (= M232), F217 (≠ M235), V238 (≠ I254), Y240 (= Y256), G261 (= G277), M264 (= M280), M265 (≠ A281)

Q8Y9N5 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
33% identity, 86% coverage: 28:296/312 of query aligns to 8:280/291 of Q8Y9N5

query
sites
Q8Y9N5

R

R

A

I

T

T

P

G

Q

H

S

T

F

E

L

L

V

I

G

G

L

L

I

I

G

A

S

T

G

P

I

I

S

R

G

H

S
|
S

L
|
L

T
x
S

P

P

A

T

M

M

H

H

E

N

E

E

E

A

G

F

S

A

R

K

L

L

G

G

L

L

H

D

Y

Y

V

V

Y

Y

R

L

R

A

I

F

D

E

L

V

D

G

A

D

Q

K

K

E

L

L

D

-

T

-

A

-

L

-

P

K

E

D

L

V

T

Q

A

G

A

F

E

R

R

A

M

M

G

N

Y

L

N

R

G

G

L

W

N

N

I

V

T

S

Y

M

P

P

C

N

K

K

Q

T

A

N

V

I

I

H

P

K

L

Y

L

L

D

D

E

K

L

L

S

S

D

P

D

A

A

A

R

E

A

L

L

V

G

G

A

A

V

V

N

N

T

T

V

V

L

V

F

N

K

D

D

D

G

G

K

V

R

L

I

T

G

G

H

H

N

I

T

T

D

D

W

G

S

T

G

G

F

Y

A

M

R

R

A

A

F

L

Q

K

R

E

G

A

L

G

P

H

D

D

V

I

S

I

L

G

E

K

R

K

V

M

V

T

Q

I

L

C

G

G

A

A

G

G

A

A

G

A

A

T

A

A

V

I

A

C

H

I

A

Q

A

A

L

A

N

L

M

D

G

G

A

V

Q

K

S

E

L

I

T

S

L

I

F

F

-
x
N

-
x
R

-
x
K

D
|
D

V

D

D

F

A

Y

A

A

R

N

A

A

Q

E

S

K

L

T

A

V

D

E

E

K

L

I

Q

N

A

S

R

K

F

T

P

D

-

C

K

K

A

A

K

Q

-

L

-

F

-

D

V

I

S

E

A

D

G

H

T

E

S

Q

L

L

E

R

E

K

S

E

L

I

A

A

A

E

A

S

Q

V

G

I

L

F

I

T

H

N

A

A

T

T

P

G

T

V

G

G

M
|
M

A

K

K

P

M

F

P

E

G

G

L

E

P

T

L

L

-

L

-

P

P

S

A

A

E

D

L

M

L

L

H

R

R

P

D

E

L

L

W

I

V

V

A

S

D

D

I

V

V

V

Y

Y

F

K

P

P

I

T

R

K

T

T

A

R

L

L

Q

E

A

I

A

A

E

E

A

E

L

Q

G

G

C

C

R

Q

T

T

L

L

S

N

G

G

G

L

G

G

M

M

A

M

V

L

Y

W

Q

Q

A

G

V

A

D

K

A

A

M

F

R

E

I

I

F

W

T

T

G

H

L

K

E

E

SLS 26:28 (≠ SLT 46:48) binding shikimate
NRKD 161:164 (≠ ---D 186) binding NAD(+)
M214 (= M232) binding NADP(+)

3tnlA 1.45 angstrom crystal structure of shikimate 5-dehydrogenase from listeria monocytogenes in complex with shikimate and NAD.
33% identity, 86% coverage: 28:296/312 of query aligns to 5:277/288 of 3tnlA

query
sites
3tnlA

R

R

A

I

T

T

P

G

Q

H

S

T

F

E

L

L

V

I

G

G

L

L

I

I

G

A

S

T

G

P

I

I

S

R

G

H

S
|
S

L

L

T
x
S

P

P

A

T

M

M

H

H

E

N

E

E

E

A

G

F

S

A

R

K

L

L

G

G

L

L

H

D

Y

Y

V

V

Y

Y

R

L

R

A

I

F

D

E

L

V

D

G

A

D

Q

K

K

E

L

L

D

-

T

-

A

-

L

-

P

K

E

D

L

V

T

Q

A

G

A

F

E

R

R

A

M

M

G

N

Y

L

N

R

G

G

L

W

N
|
N

I

V

T
x
S

Y
x
M

P

P

C

N

K
|
K

Q

T

A

N

V

I

I

H

P

K

L

Y

L

L

D

D

E

K

L

L

S

S

D

P

D

A

A

A

R

E

A

L

L

V

G

G

A

A

V

V

N
|
N

T

T

V

V

L

V

F

N

K

D

D

D

G

G

K

V

R

L

I

T

G

G

H

H

N

I

T

T

D
|
D

W

G

S

T

G

G

F

Y

A

M

R

R

A

A

F

L

Q

K

R

E

G

A

L

G

P

H

D

D

V

I

S

I

L

G

E

K

R

K

V

M

V

T

Q

I

L

C

G
|
G

A
|
A

G
|
G

A
|
A

G

A

A

T

A

A

V

I

A

C

H

I

A

Q

A

A

L

A

N

L

M

D

G

G

A

V

Q

K

S

E

L

I

T

S

L

I

F

F

-
x
N

-
x
R

-

K

D
|
D

V

D

D
x
F

A

Y

A

A

R

N

A

A

Q

E

S

K

L

T

A

V

D

E

E

K

L

I

Q

N

A

S

R

K

F

T

P

D

-

C

K

K

A

A

K

Q

-

L

-

F

-

D

V

I

S

E

A

D

G

H

T

E

S

Q

L

L

E

R

E

K

S

E

L

I

A

A

A

E

A

S

Q

V

G

I

L

F

I

T

H

N

A

A

T
|
T

P

G

T
x
V

G

G

M
|
M

A

K

K

P

M
x
F

P

E

G

G

L

E

P

T

L

L

-

L

-

P

P

S

A

A

E

D

L

M

L

L

H

R

R

P

D

E

L

L

W

I

V

V

A

S

D

D

I
x
V

V

V

Y
|
Y

F

K

P

P

I

T

R

K

T

T

A

R

L

L

Q

E

A

I

A

A

E

E

A

E

L

Q

G

G

C

C

R

Q

T

T

L

L

S

N

G

G

G

L

G

G

M
|
M

A
x
M

V

L

Y

W

Q
|
Q

A

G

V

A

D

K

A

A

M

F

R

E

I

I

F

W

T

T

G

H

L

K

E

E

binding nicotinamide-adenine-dinucleotide: M71 (≠ Y99), G134 (= G162), A135 (= A163), G136 (= G164), G137 (= G165), A138 (= A166), N158 (vs. gap), R159 (vs. gap), D161 (= D186), F163 (≠ D188), T207 (= T228), V209 (≠ T230), M211 (= M232), F214 (≠ M235), V235 (≠ I254), Y237 (= Y256), M261 (= M280), M262 (≠ A281)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S23 (= S46), S25 (≠ T48), N68 (= N96), S70 (≠ T98), K74 (= K102), N95 (= N123), D110 (= D138), Q265 (= Q284)

Q58484 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
33% identity, 86% coverage: 32:300/312 of query aligns to 5:270/282 of Q58484

query
sites
Q58484

Q

K

S

T

F

K

L

V

V

I

G

G

L

L

I

I

G

G

S

H

G

P

I

V

S

E

G

H

S

S

L

F

T

S

P

P

A

I

M

M

H

H

E

N

E

A

G

F

S

K

R

D

L

K

G

G

L

L

H

N

Y

Y

V

V

Y

Y

R

V

R

A

I

F

D

D

L

V

D

-

A

-

Q

-

K

-

L

-

D

-

T

-

A

-

L

L

P

P

E

E

-

N

-

L

-

K

-

Y

L

V

L

I

T

D

A

G

A

A

E

K

R

A

M

L

G

G

Y

I

N

V

G

G

L

F

N

N

I

V

T

T

Y

I

P

P

C

H

K

K

Q

I

A

E

V

I

I

M

P

K

L

Y

L

L

D

D

E

E

L

I

S

D

D

K

D

D

A

A

R

Q

A

L

L

I

G

G

A

A

V

V

N

N

T

T

V

I

L

K

F

I

K

E

D

D

G

G

K

K

R

A

I

I

G

G

H

Y

N

N

T

T

D

D

W

G

S

I

G

G

F

A

A

R

R

M

A

A

F

L

Q

E

R

E

G

E

L

I

P

G

D

R

V

V

S

K

L

D

E

K

R

N

V

I

V

V

Q

I

L

Y

G
|
G

A
|
A

G
|
G

A
|
A

G

A

A

R

A

A

V

V

A

A

H

F

A

E

A

L

L

A

N

K

M

-

G

-

A

D

Q

N

S

N

L

I

T

I

L

I

F

A

D
x
N

V
x
R

D
x
T

A
x
V

A
x
E

R
x
K

A

A

Q

E

S

A

L

L

A

A

D

K

E

E

L

I

Q

A

A

E

R

K

F

L

P

N

K

K

-

K

-

F

A

G

K

E

V

E

S

V

A

K

G

F

T

S

S

G

L

L

E

D

E

V

S

D

L

L

A

D

A

G

A

V

Q

D

G

I

L

I

I

I

H

N

A

A

T
|
T

P

P

T

I

G

G

M
|
M

A

-

K

-

M

Y

P

P

G

N

L

I

P

D

-

V

-

E

-

P

-

I

L

V

P

K

A

A

E

E

L

K

L

L

H

R

R

E

D

D

L

M

W

V

V

V

A

M

D

D

I
x
L

V

I

Y

Y

F

N

P

P

I

L

R

E

T

T

A

V

L

L

Q

K

A

E

A

A

E

K

A

K

L

V

G

N

C

A

R

K

T

T

L

I

S

N

G

G

G

L

G

G

M

M

A

L

V

I

Y

Y

Q

Q

A

G

V

A

D

V

A

A

M

F

R

K

I

I

F

W

T

T

G

G

L

V

E

E

P

P

D

N

A

I

E

E

GAGGA 130:134 (= GAGGA 162:166) binding NADP(+)
NRTVEK 152:157 (≠ DVDAAR 186:191) binding NADP(+)
T196 (= T228) binding NADP(+)
M200 (= M232) binding NADP(+)
L224 (≠ I254) binding NADP(+)

1nvtB Crystal structure of shikimate dehydrogenase (aroe or mj1084) in complex with NADP+ (see paper)
33% identity, 86% coverage: 32:300/312 of query aligns to 10:275/287 of 1nvtB

query
sites
1nvtB

Q

K

S

T

F

K

L

V

V

I

G

G

L

L

I

I

G

G

S

H

G

P

I

V

S
x
E

G
x
H

S

S

L

F

T

S

P

P

A

I

M

M

H

H

E

N

E

A

G

F

S

K

R

D

L

K

G

G

L

L

H

N

Y

Y

V

V

Y

Y

R

V

R

A

I

F

D

D

L

V

D

-

A

-

Q

-

K

-

L

-

D

-

T

-

A

-

L

L

P

P

E

E

-

N

-

L

-

K

-

Y

L

V

L

I

T

D

A

G

A

A

E

K

R

A

M

L

G

G

Y

I

N

V

G

G

L

F

N

N

I

V

T

T

Y
x
I

P

P

C

H

K
|
K

Q

I

A

E

V

I

I

M

P

K

L

Y

L

L

D

D

E

E

L

I

S

D

D

K

D

D

A

A

R

Q

A

L

L

I

G

G

A

A

V

V

N

N

T

T

V

I

L

K

F

I

K

E

D

D

G

G

K

K

R

A

I

I

G

G

H

Y

N

N

T

T

D
|
D

W

G

S

I

G

G

F

A

A

R

R

M

A

A

F

L

Q

E

R

E

G

E

L

I

P

G

D

R

V

V

S

K

L

D

E

K

R

N

V

I

V

V

Q

I

L

Y

G
|
G

A

A

G
|
G

A
|
A

G

A

A

R

A

A

V

V

A

A

H

F

A

E

A

L

L

A

N

K

M

-

G

-

A

D

Q

N

S

N

L

I

T

I

L

I

F

A

D
x
N

V
x
R

D
x
T

A

V

A

E

R
x
K

A

A

Q

E

S

A

L

L

A

A

D

K

E

E

L

I

Q

A

A

E

R

K

F

L

P

N

K

K

-

K

-

F

A

G

K

E

V

E

S

V

A

K

G

F

T

S

S

G

L

L

E

D

E

V

S

D

L

L

A

D

A

G

A

V

Q

D

G

I

L

I

I

I

H

N

A
|
A

T
|
T

P
|
P

T
x
I

G

G

M
|
M

A

-

K

-

M

Y

P

P

G

N

L

I

P

D

-

V

-

E

-

P

-

I

L

V

P

K

A

A

E

E

L

K

L

L

H

R

R

E

D

D

L

M

W

V

V

V

A

M

D

D

I
x
L

V

I

Y
|
Y

F

N

P

P

I

L

R

E

T

T

A

V

L

L

Q

K

A

E

A

A

E

K

A

K

L

V

G

N

C

A

R

K

T

T

L

I

S

N

G

G

G

L

G

G

M
|
M

A
x
L

V

I

Y

Y

Q

Q

A

G

V

A

D

V

A

A

M

F

R

K

I

I

F

W

T

T

G

G

L

V

E

E

P

P

D

N

A

I

E

E

active site: K75 (= K102), D111 (= D138)
binding nadp nicotinamide-adenine-dinucleotide phosphate: I72 (≠ Y99), G135 (= G162), G137 (= G164), G138 (= G165), A139 (= A166), N157 (≠ D186), R158 (≠ V187), T159 (≠ D188), K162 (≠ R191), A200 (= A227), T201 (= T228), P202 (= P229), I203 (≠ T230), M205 (= M232), L229 (≠ I254), Y231 (= Y256), M255 (= M280), L256 (≠ A281)
binding zinc ion: E22 (≠ S44), H23 (≠ G45)

1nvtA Crystal structure of shikimate dehydrogenase (aroe or mj1084) in complex with NADP+ (see paper)
33% identity, 86% coverage: 32:300/312 of query aligns to 10:275/287 of 1nvtA

query
sites
1nvtA

Q

K

S

T

F

K

L

V

V

I

G

G

L

L

I

I

G

G

S

H

G

P

I

V

S

E

G

H

S

S

L

F

T

S

P

P

A

I

M

M

H

H

E

N

E

A

G

F

S

K

R

D

L

K

G

G

L

L

H

N

Y

Y

V

V

Y

Y

R

V

R

A

I

F

D

D

L

V

D

-

A

-

Q

-

K

-

L

-

D

-

T

-

A

-

L

L

P

P

E

E

-

N

-

L

-

K

-

Y

L

V

L

I

T

D

A

G

A

A

E

K

R

A

M

L

G

G

Y

I

N

V

G

G

L

F

N

N

I

V

T

T

Y

I

P

P

C

H

K
|
K

Q

I

A

E

V

I

I

M

P

K

L

Y

L

L

D

D

E

E

L

I

S

D

D

K

D

D

A

A

R

Q

A

L

L

I

G

G

A

A

V

V

N

N

T

T

V

I

L

K

F

I

K

E

D

D

G

G

K

K

R

A

I

I

G

G

H

Y

N

N

T

T

D
|
D

W

G

S

I

G

G

F

A

A

R

R

M

A

A

F

L

Q

E

R

E

G

E

L

I

P

G

D

R

V

V

S

K

L

D

E

K

R

N

V

I

V

V

Q

I

L

Y

G
|
G

A

A

G

G

A
|
A

G

A

A

R

A

A

V

V

A

A

H

F

A

E

A

L

L

A

N

K

M

-

G

-

A

D

Q

N

S

N

L

I

T

I

L

I

F

A

D
x
N

V
x
R

D
x
T

A

V

A

E

R
x
K

A

A

Q

E

S

A

L

L

A

A

D

K

E

E

L

I

Q

A

A

E

R

K

F

L

P

N

K

K

-

K

-

F

A

G

K

E

V

E

S

V

A

K

G

F

T

S

S

G

L

L

E

D

E

V

S

D

L

L

A

D

A

G

A

V

Q

D

G

I

L

I

I

I

H

N

A
|
A

T
|
T

P
|
P

T
x
I

G

G

M
|
M

A

-

K

-

M

Y

P

P

G

N

L

I

P

D

-

V

-

E

-

P

-

I

L

V

P

K

A

A

E

E

L

K

L

L

H

R

R

E

D

D

L

M

W

V

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active site: K75 (= K102), D111 (= D138)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G135 (= G162), A139 (= A166), N157 (≠ D186), R158 (≠ V187), T159 (≠ D188), K162 (≠ R191), A200 (= A227), T201 (= T228), P202 (= P229), I203 (≠ T230), M205 (= M232), L229 (≠ I254), Y231 (= Y256), G252 (= G277), M255 (= M280), L256 (≠ A281)

Q5HNV1 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Staphylococcus epidermidis (strain ATCC 35984 / RP62A) (see paper)
32% identity, 84% coverage: 38:300/312 of query aligns to 5:255/269 of Q5HNV1

query
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Q5HNV1

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SLS 13:15 (≠ SLT 46:48) binding shikimate
T60 (= T98) binding shikimate
N85 (= N123) binding shikimate
D100 (= D138) binding shikimate
Y211 (= Y256) Plays a major role in the catalytic process and a minor role in the substrate binding; mutation to F: Leads to a 345-fold decrease in the catalytic efficiency and a 3-fold decrease in the affinity binding for shikimate.
Q239 (= Q284) binding shikimate

3dooA Crystal structure of shikimate dehydrogenase from staphylococcus epidermidis complexed with shikimate (see paper)
31% identity, 84% coverage: 38:300/312 of query aligns to 5:246/258 of 3dooA

query
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binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S13 (= S46), S15 (≠ T48), N58 (= N96), T60 (= T98), K64 (= K102), N85 (= N123), D100 (= D138), F227 (≠ A281), Q230 (= Q284)

1npdB X-ray structure of shikimate dehydrogenase complexed with NAD+ from e.Coli (ydib) northeast structural genomics research consortium (nesg) target er24 (see paper)
30% identity, 88% coverage: 27:302/312 of query aligns to 1:280/288 of 1npdB

query
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