SitesBLAST
Comparing WP_013092939.1 NCBI__GCF_000092885.1:WP_013092939.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5bntC X-ray crystal structure of a aspartate-semialdehyde dehydrogenase bound to NADP from pseudomonas aeruginosa
71% identity, 100% coverage: 2:373/373 of query aligns to 4:371/371 of 5bntC
- active site: C136 (= C134), Q163 (= Q161), R269 (= R271), H276 (= H278)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G9 (= G7), R11 (= R9), G12 (= G10), M13 (= M11), V14 (= V12), T38 (= T36), S39 (= S37), Q74 (= Q72)
7tcmA Crystal structure of aspartate-semialdehyde dehydrogenase from acinetobacter baumannii in complex with NADP
71% identity, 100% coverage: 1:372/373 of query aligns to 3:370/373 of 7tcmA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G9 (= G7), R11 (= R9), G12 (= G10), M13 (= M11), V14 (= V12), T38 (= T36), S39 (= S37), C74 (= C71), Q75 (= Q72), G76 (= G73), A100 (= A97), S167 (= S164), Q352 (= Q354), A357 (= A359)
3pzrA Crystals structure of aspartate beta-semialdehyde dehydrogenase from vibrio cholerae with NADP and product of s-carbamoyl-l-cysteine (see paper)
68% identity, 99% coverage: 1:371/373 of query aligns to 1:367/370 of 3pzrA
- active site: C134 (= C134), Q161 (= Q161), R267 (= R271), H274 (= H278)
- binding cysteine: C134 (= C134), Q161 (= Q161), G165 (= G165), E240 (= E240), R267 (= R271), H274 (= H278), Q350 (= Q354)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G7 (= G7), R9 (= R9), G10 (= G10), M11 (= M11), V12 (= V12), S35 (= S35), T36 (= T36), S37 (= S37)
Q9KQG2 Aspartate-semialdehyde dehydrogenase 1; ASA dehydrogenase 1; ASADH 1; Aspartate-beta-semialdehyde dehydrogenase 1; EC 1.2.1.11 from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) (see paper)
68% identity, 99% coverage: 1:371/373 of query aligns to 1:367/370 of Q9KQG2
- RGMV 9:12 (= RGMV 9:12) binding NADP(+)
- TS 36:37 (= TS 36:37) binding NADP(+)
- Q72 (= Q72) binding NADP(+)
- C134 (= C134) active site, Acyl-thioester intermediate
- SG 164:165 (= SG 164:165) binding NADP(+)
- P192 (= P192) binding NADP(+)
- Q350 (= Q354) binding NADP(+)
4r5mA Crystal structure of vc-aspartate beta-semialdehyde-dehydrogenase with NADP and 4-nitro-2-phosphono-benzoic acid (see paper)
68% identity, 99% coverage: 1:371/373 of query aligns to 1:367/369 of 4r5mA
- active site: C134 (= C134), Q161 (= Q161), R267 (= R271), H274 (= H278)
- binding 4-nitro-2-phosphonobenzoic acid: C71 (= C71), G73 (= G73), G74 (= G74), A96 (= A96), A97 (= A97), S98 (= S98), R101 (= R101), K243 (= K243)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G7 (= G7), R9 (= R9), G10 (= G10), M11 (= M11), V12 (= V12), T36 (= T36), S37 (= S37), Q72 (= Q72), G73 (= G73), G165 (= G165)
1mb4A Crystal structure of aspartate semialdehyde dehydrogenase from vibrio cholerae with NADP and s-methyl-l-cysteine sulfoxide (see paper)
68% identity, 99% coverage: 1:371/373 of query aligns to 1:367/369 of 1mb4A
- active site: C134 (= C134), Q161 (= Q161), R267 (= R271), H274 (= H278)
- binding cysteine: N133 (= N133), C134 (= C134), G165 (= G165), E240 (= E240), R267 (= R271), H274 (= H278)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G7 (= G7), R9 (= R9), G10 (= G10), M11 (= M11), V12 (= V12), T36 (= T36), S37 (= S37), C71 (= C71), Q72 (= Q72), G73 (= G73), A97 (= A97), S164 (= S164), Q350 (= Q354), L351 (= L355), A355 (= A359)
1gl3A Aspartate beta-semialdehyde dehydrogenase in complex with NADP and substrate analogue s-methyl cysteine sulfoxide (see paper)
69% identity, 98% coverage: 2:368/373 of query aligns to 3:364/367 of 1gl3A
- active site: C135 (= C134), Q162 (= Q161), R267 (= R271), H274 (= H278)
- binding cysteine: C135 (= C134), Q162 (= Q161), I230 (= I229), E241 (= E240), R267 (= R271), H274 (= H278)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R10 (= R9), G11 (= G10), M12 (= M11), V13 (= V12), T37 (= T36), S38 (= S37), C72 (= C71), Q73 (= Q72), A98 (= A97), C135 (= C134), S165 (= S164), G166 (= G165), G168 (= G167), A169 (= A168), Q350 (= Q354), A355 (= A359)
P0A9Q9 Aspartate-semialdehyde dehydrogenase; ASA dehydrogenase; ASADH; Aspartate-beta-semialdehyde dehydrogenase; EC 1.2.1.11 from Escherichia coli (strain K12) (see 2 papers)
69% identity, 98% coverage: 2:368/373 of query aligns to 3:364/367 of P0A9Q9
- RGMV 10:13 (= RGMV 9:12) binding NADP(+)
- TS 37:38 (= TS 36:37) binding NADP(+)
- Q73 (= Q72) binding NADP(+)
- C135 (= C134) active site, Acyl-thioester intermediate; mutation to A: Complete loss of activity.; mutation to S: 99.7% loss of activity.
- SGGGA 165:169 (≠ SGAGA 164:168) binding NADP(+)
- R173 (= R172) binding NADP(+)
- P193 (= P192) binding NADP(+)
- Q350 (= Q354) binding NADP(+)
P44801 Aspartate-semialdehyde dehydrogenase; ASA dehydrogenase; ASADH; Aspartate-beta-semialdehyde dehydrogenase; EC 1.2.1.11 from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) (see 2 papers)
68% identity, 98% coverage: 2:368/373 of query aligns to 3:367/371 of P44801
- RGMV 10:13 (= RGMV 9:12) binding NADP(+)
- TS 37:38 (= TS 36:37) binding NADP(+)
- Q74 (= Q72) binding NADP(+)
- R103 (= R101) binding phosphate; mutation to K: 2-fold increase in affinity for ASA, 23-fold decrease in affinity for phosphate, and 275-fold decrease in activity.; mutation to L: 7-fold increase in affinity for ASA, 150-fold decrease in affinity for phosphate, and 1400-fold decrease in activity.
- C136 (= C134) active site, Acyl-thioester intermediate
- S166 (= S164) binding NADP(+)
- E243 (= E240) mutation to D: No change in affinity for ASA and 82-fold decrease in activity.
- K246 (= K243) binding phosphate; mutation to R: 2-fold increase in affinity for ASA, nearly no change in affinity for phosphate, and 30-fold decrease in activity.
- R270 (= R271) mutation to K: 2-fold decrease in affinity for ASA and 825-fold decrease in activity.
- H277 (= H278) active site, Proton acceptor
- Q353 (= Q354) binding NADP(+)
1pquA Crystal structure of the h277n mutant of aspartate semialdehyde dehydrogenase from haemophilus influenzae bound with NADP, s-methyl cysteine sulfoxide and cacodylate (see paper)
68% identity, 98% coverage: 2:368/373 of query aligns to 3:367/371 of 1pquA
- active site: C136 (= C134), Q163 (= Q161), R270 (= R271), N277 (≠ H278)
- binding cysteine: C136 (= C134), Q163 (= Q161), G167 (= G165), E243 (= E240), R270 (= R271)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G7), R10 (= R9), G11 (= G10), M12 (= M11), V13 (= V12), T36 (≠ S35), T37 (= T36), S38 (= S37), C73 (= C71), Q74 (= Q72), A99 (= A97), S166 (= S164), L354 (= L355), A358 (= A359)
1tb4A Crystal structure of aspartate-semialdehyde dehydrogenase from haemophilus influenzae with a bound periodate (see paper)
67% identity, 98% coverage: 2:368/373 of query aligns to 3:353/357 of 1tb4A
1ta4A Crystal structure of aspartate-semialdehyde dehydrogenase from haemophilus influenzae with a bound arsenate (see paper)
67% identity, 98% coverage: 2:368/373 of query aligns to 3:353/357 of 1ta4A