SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013093107.1 NCBI__GCF_000092885.1:WP_013093107.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P33160 Formate dehydrogenase; FDH; NAD-dependent formate dehydrogenase; EC 1.17.1.9 from Pseudomonas sp. (strain 101) (Achromobacter parvulus T1) (see 3 papers)
73% identity, 100% coverage: 1:386/386 of query aligns to 1:385/401 of P33160

query
sites
P33160

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M1 (= M1) modified: Initiator methionine, Removed
I123 (= I124) binding substrate
N147 (= N148) binding substrate
S148 (= S149) binding NAD(+)
RI 202:203 (= RI 203:204) binding NAD(+)
D222 (= D223) binding NAD(+)
C256 (≠ S257) mutation C->S,M: High resistance to inactivation by Hg(2+), Increased stability at 25 degree Celsius and decreased thermostability at 45 degree Celsius.
PLHPE 257:261 (≠ PLYPS 258:262) binding NAD(+)
T283 (≠ C284) binding NAD(+)
D309 (= D310) binding NAD(+)
HISG 333:336 (= HISG 334:337) binding NAD(+)
S381 (= S382) binding NAD(+)

2nadA High resolution structures of holo and apo formate dehydrogenase (see paper)
73% identity, 100% coverage: 2:386/386 of query aligns to 1:384/391 of 2nadA

query
sites
2nadA

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active site: N146 (= N148), R284 (= R286), D308 (= D310), Q313 (= Q315), H332 (= H334)
binding azide ion: P97 (= P99), F98 (= F100), I122 (= I124), N146 (= N148), R284 (= R286)
binding nicotinamide-adenine-dinucleotide: I122 (= I124), N146 (= N148), V150 (= V152), A198 (≠ G200), G200 (= G202), R201 (= R203), I202 (= I204), D221 (= D223), P256 (= P258), E260 (≠ S262), T261 (= T263), T282 (≠ C284), A283 (= A285), R284 (= R286), D308 (= D310), H332 (= H334), S334 (= S336), G335 (= G337), S380 (= S382)

6jujA Crystal structure of formate dehydrogenase mutant v198i/c256i/p260s/e261p/s381n/s383f from pseudomonas sp. 101in complex with non-natural cofactor nicotinamide cytosine dinucleotide (see paper)
73% identity, 99% coverage: 2:383/386 of query aligns to 1:381/381 of 6jujA

query
sites
6jujA

A

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binding [[(2S,3S,4R,5S)-5-(3-aminocarbonylpyridin-1-ium-1-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2S,3S,4R,5S)-5-(4-azanyl-2-oxidanylidene-pyrimidin-1-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl hydrogen phosphate: F98 (= F100), N146 (= N148), V150 (= V152), G200 (= G202), I202 (= I204), D221 (= D223), R222 (≠ P224), I255 (≠ S257), P256 (= P258), H258 (≠ Y260), T282 (≠ C284), A283 (= A285), D308 (= D310), H332 (= H334), S334 (= S336), G335 (= G337)

6t9wB Crystal structure of formate dehydrogenase fdh2 d222a/q223r enzyme from granulicella mallensis mp5actx8 in complex with NADP and azide.
71% identity, 99% coverage: 2:385/386 of query aligns to 1:383/383 of 6t9wB

query
sites
6t9wB

A

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R

R

T

A

D

D

V

V

P

P

S

K

I

I

A

D

S

H

Y

Y

P

P

G

G

Q

Q

R

T

P

A

P

P

S

T

P

P

K

K

G

-

P

Q

L

I

G

D

F

F

K

T

P

P

G

G

E

E

L

L

V

L

G

G

S

S

V

V

S

S

G

G

E

E

L

L

G

G

L

L

R

R

H

K

Y

Y

L

L

E

E

S

G

Q

L

G

G

H

H

E

T

L

L

I

V

V

V

T

T

S

S

D

D

K

K

D

E

G

G

P

E

D

D

S

S

I

V

F

F

E

E

R

R

H

E

L

L

C

P

D

D

A

A

D

E

I

I

V

V

I

I

S

S

Q

Q

P
|
P

F
|
F

W

W

P

P

A

A

Y

Y

L

L

T

T

R

P

E

E

R

R

I

I

A

A

K

K

A

A

K

K

L

L

K

K

L

L

A

A

L

V

T

T

A

A

G
|
G

I
|
I

G

G

S

S

D

D

H

H

V

V

D

D

L

L

Q

E

A

A

A

I

E

K

R

N

D

G

I

I

T

T

V

V

A

A

E

E

V

V

T

T

F

Y

S

S

N
|
N

S
|
S

I

I

S

S

V
|
V

A

S

E

E

H

H

V

V

M

M

T

M

V

I

L

L

A

S

L

L

V

V

R

R

N

N

Y

Y

L

I

P

P

S

S

H

Y

Q

Q

Y

W

A

V

V

I

N

K

G

G

W

W

N

N

I

I

A

A

D

D

C

C

V

V

A

E

R

R

S

S

Y

Y

D

D

L

L

E

E

G

A

M

M

H

H

F

V

G

G

T

T

I

V

G
x
A

A

A

G
|
G

R
|
R

I
|
I

G

G

L

L

A

A

V

V

L

L

R

K

R

R

L

L

K

K

P

P

F

F

D

D

V

V

H

K

L

L

H

H

Y

Y

Y
x
F

D

A

P
x
R

H

H

R

R

L

L

S

P

P

E

E

S

L

V

E

E

R

N

E

E

L

L

N

G

V

L

T

T

F

Y

H

H

S

P

S

S

A

V

E

E

S

D

L

M

V

V

R

K

V

V

C

C

D

D

V

V

I

V

N

T

L

I

Q

N

S

A

P
|
P

L

L

Y
x
H

P

P

S

G

T
|
T

E

L

H

D

M

L

F

F

N

N

D

D

A

E

M

L

L

I

A

S

Q

K

I

M

K

K

P

R

G

G

A

A

Y

Y

L

L

I

V

N

N

C
x
T

A
|
A

R
|
R

G

G

K

K

L

I

C

C

D

N

A

R

D

D

A

A

V

V

A

V

R

R

A

A

L

L

Q

E

S

S

G

G

R

Q

L

L

A

A

G

G

Y

Y

G

A

G

G

D
|
D

V

V

W

W

F

F

P

P

Q

Q

P

P

A

A

P

P

A

K

E

D

H

H

P

P

W

W

R

R

S

T

M

M

P

P

N

H

E

H

G

G

M

M

T

T

P

P

H
|
H

I

I

S
|
S

G
|
G

A

T

S

S

L

L

S

S

A

A

Q

Q

A

A

R

R

Y

Y

A

A

G

G

T

T

R

R

E

E

I

I

L

L

E

E

C

C

F

W

L

F

E

E

G

E

R

R

A

P

I

I

R

R

A

E

E

E

Y

Y

L

L

I

I

V

V

N

D

G

G

K

K

L

L

A

A

G

G

T

T

G

G

A

A

A

H

S
|
S

Y

Y

K

T

L

V

binding azide ion: P97 (= P99), F98 (= F100), G121 (= G123), I122 (= I124), H332 (= H334)
binding nadp nicotinamide-adenine-dinucleotide phosphate: I122 (= I124), N146 (= N148), S147 (= S149), V150 (= V152), A198 (≠ G200), G200 (= G202), R201 (= R203), I202 (= I204), F220 (≠ Y222), R222 (≠ P224), P256 (= P258), H258 (≠ Y260), T261 (= T263), T282 (≠ C284), A283 (= A285), R284 (= R286), D308 (= D310), H332 (= H334), S334 (= S336), G335 (= G337), S380 (= S382)

2gsdA NAD-dependent formate dehydrogenase from bacterium moraxella sp.C2 in complex with NAD and azide (see paper)
71% identity, 100% coverage: 2:386/386 of query aligns to 1:384/399 of 2gsdA

query
sites
2gsdA

A

A

K

K

I

V

L

V

C

C

A

V

L

L

F

Y

P

D

D

D

P

P

V

I

T

N

G

G

Y

Y

P

P

P

T

S

S

Y

Y

A

A

R

R

T

D

D

D

V

L

P

P

S

R

I

I

A

D

S

K

Y

Y

P

P

G

D

G

G

Q

Q

R

T

P

L

P

P

S

T

P

P

K

K

G

A

P

-

L

I

G

D

F

F

K

T

P

P

G

G

E

A

L

L

V

L

G

G

S

S

V

V

S

S

G

G

E

E

L

L

G

G

L

L

R

R

H

K

Y

Y

L

L

E

E

S

S

Q

Q

G

G

H

H

E

E

L

L

I

V

V

V

T

T

S

S

D

S

K

K

D

D

G

G

P

P

D

D

S

S

I

E

F

L

E

E

R

K

H

H

L

L

C

H

D

D

A

A

D

E

I

V

V

I

I

I

S

S

Q

Q

P
|
P

F
|
F

W

W

P

P

A

A

Y

Y

L

L

T

T

R

A

E

E

R

R

I

I

A

A

K

K

A

A

K

P

K

K

L

L

K

K

L

L

A

A

L

L

T

T

A

A

G

G

I
|
I

G

G

S

S

D

D

H

H

V

V

D

D

L

L

Q

Q

A

A

A

I

E

D

R

N

D

N

I

I

T

T

V

V

A

A

E

E

V

V

T

T

F

Y

S

C

N
|
N

S

S

I

N

S

S

V
|
V

A

A

E

E

H

H

V

V

M

M

T

M

V

V

L

L

A

G

L

L

V

V

R

R

N

N

Y

Y

L

I

P

P

S

S

H

H

Q

D

Y

W

A

A

V

R

N

N

G

G

W

W

N

N

I

I

A

A

D

D

C

C

V

V

A

A

R

R

S

S

Y

Y

D

D

L

V

E

E

G

G

M

M

H

H

F

V

G

G

T

T

I

V

G
x
A

A

A

G
|
G

R
|
R

I
|
I

G

G

L

L

A

R

V

V

L

L

R

R

R

L

L

L

K

A

P

P

F

F

D

D

V

M

H

H

L

L

H

H

Y

Y

Y

T

D
|
D

P
x
R

H

H

R

R

L

L

S

P

P

E

E

A

L

V

E

E

R

K

E

E

L

L

N

N

V

L

T

T

F

W

H

H

S

A

S

T

A

R

E

E

S

D

L

M

V

Y

R

G

V

A

C

C

D

D

V

V

I

V

N

T

L

L

Q

N

S

C

P
|
P

L

L

Y
x
H

P

P

S

E

T
|
T

E

E

H

H

M

M

F

I

N

N

D

D

A

E

M

T

L

L

A

K

Q

L

I

F

K

K

P

R

G

G

A

A

Y

Y

L

L

I

V

N

N

C
x
T

A
|
A

R
|
R

G

G

K

K

L

L

C

C

D

D

A

R

D

D

A

A

V

I

A

V

R

R

A

A

L

L

Q

E

S

S

G

G

R

R

L

L

A

A

G

G

Y

Y

G

A

G

G

D
|
D

V

V

W

W

F

F

P

P

Q
|
Q

P

P

A

A

P

P

A

N

E

D

H

H

P

P

W

W

R

R

S

T

M

M

P

P

N

H

E

N

G

G

M

M

T

T

P

P

H
|
H

I

I

S
|
S

G
|
G

A

T

S

S

L

L

S

S

A

A

Q

Q

A

T

R

R

Y

Y

A

A

G

G

T

T

R

R

E

E

I

I

L

L

E

E

C

C

F

Y

L

F

E

E

G

G

R

R

A

P

I

I

R

R

A

D

E

E

Y

Y

L

L

I

I

V

V

N

Q

G

G

K

G

L

L

A

A

G

G

T

V

G

G

A

A

A

H

S
|
S

Y

Y

K

S

L

K

G

G

active site: N146 (= N148), R284 (= R286), D308 (= D310), Q313 (= Q315), H332 (= H334)
binding azide ion: P97 (= P99), F98 (= F100), I122 (= I124), R284 (= R286), H332 (= H334)
binding nicotinamide-adenine-dinucleotide: I122 (= I124), N146 (= N148), V150 (= V152), A198 (≠ G200), G200 (= G202), R201 (= R203), I202 (= I204), D221 (= D223), R222 (≠ P224), P256 (= P258), H258 (≠ Y260), T261 (= T263), T282 (≠ C284), A283 (= A285), R284 (= R286), D308 (= D310), H332 (= H334), S334 (= S336), G335 (= G337), S380 (= S382)

3wr5A Structural basis on the efficient co2 reduction of acidophilic formate dehydrogenase
70% identity, 100% coverage: 1:386/386 of query aligns to 5:389/401 of 3wr5A

query
sites
3wr5A

M

M

A

A

K

K

I

I

L

L

C

C

A

V

L

L

F

Y

P

D

D

D

P

P

V

V

T

D

G

G

Y

Y

P

P

P

K

S

T

Y

Y

A

A

R

R

T

D

D

D

V

L

P

P

S

K

I

I

A

D

S

H

Y

Y

P

P

G

G

Q

Q

R

T

P

L

P

P

S

T

P

P

K

K

G

A

P

-

L

I

G

D

F

F

K

T

P

P

G

G

E

Q

L

L

V

L

G

G

S

S

V

V

S

S

G

G

E

E

L

L

G

G

L

L

R

R

H

K

Y

Y

L

L

E

E

S

A

Q

N

G

G

H

H

E

T

L

F

I

V

V

V

T

T

S

S

D

D

K

K

D

D

G

G

P

P

D

D

S

S

I

V

F

F

E

E

R

K

H

E

L

L

C

V

D

D

A

A

D

D

I

V

V

V

I

I

S

S

Q

Q

P

P

F

F

W

W

P

P

A

A

Y

Y

L

L

T

T

R

P

E

E

R

R

I

I

A

A

K

K

A

A

K

K

K

N

L

L

K

K

L

L

A

A

L

L

T

T

A

A

G

G

I

I

G

G

S

S

D

D

H

H

V

V

D

D

L

L

Q

Q

A

S

A

A

A

I

E

D

R

R

D

G

I

I

T

T

V

V

A

A

E

E

V

V

T

T

F

Y

S

C

N
|
N

S

S

I

I

S

S

V
|
V

A

A

E

E

H

H

V

V

M

M

T

M

V

I

L

L

A

G

L

L

V

V

R

R

N

N

Y

Y

L

I

P

P

S

S

H

H

Q

D

Y

W

A

A

V

R

N

K

G

G

W

W

N

N

I

I

A

A

D

D

C

C

V

V

A

E

R

H

S

S

Y

Y

D

D

L

L

E

E

G

G

M

M

H

T

F

V

G

G

T

S

I

V

G
x
A

A

A

G
|
G

R
|
R

I
|
I

G

G

L

L

A

A

V

V

L

L

R

R

L

L

K

A

P

P

F

F

D

D

V

V

H

K

L

L

H

H

Y

Y

Y

T

D
|
D

P
x
R

H

H

R

R

L

L

S

P

P

E

E

A

L

V

E

E

R

K

E

E

L

L

N

G

V

L

T

V

F

W

H

H

S

D

S

T

A

R

E

E

S

D

L

M

V

Y

R

P

V

H

C

C

D

D

V

V

I

V

N

T

L

L

Q

N

S
x
V

P
|
P

L

L

Y

H

P

P

S

E

T

T

E

E

H

H

M

M

F

I

N

N

D

D

A

E

M

T

L

L

A

K

Q

L

I

F

K

K

P

R

G

G

A

A

Y

Y

L

I

I

V

N

N

C
x
T

A
|
A

R
|
R

G

G

K

K

L

L

C

A

D

D

A

R

D

D

A

A

V

I

A

V

R

R

A

A

L

I

Q

E

S

S

G

G

R

Q

L

L

A

A

G

G

Y

Y

G

A

G

G

D
|
D

V

V

W

W

F

F

P

P

Q
|
Q

P

P

A

A

P

P

A

K

E

D

H

H

P

P

W

W

R

R

S

T

M

M

P

K

N

W

E

E

G

G

M

M

T

T

P

P

H
|
H

I

I

S
|
S

G
|
G

A

T

S

S

L

L

S

S

A

A

Q

Q

A

A

R

R

Y

Y

A

A

G

G

T

T

R

R

E

E

I

I

L

L

E

E

C

C

F

F

L

F

E

E

G

G

R

R

A

P

I

I

R

R

A

D

E

E

Y

Y

L

L

I

I

V

V

N

Q

G

G

K

A

L

L

A

A

G

G

T

T

G

G

A

A

A

H

S
|
S

Y

Y

K

S

L

K

G

G

active site: N151 (= N148), R289 (= R286), D313 (= D310), Q318 (= Q315), H337 (= H334)
binding nicotinamide-adenine-dinucleotide: N151 (= N148), V155 (= V152), A203 (≠ G200), G205 (= G202), R206 (= R203), I207 (= I204), D226 (= D223), R227 (≠ P224), V260 (≠ S257), P261 (= P258), T287 (≠ C284), A288 (= A285), R289 (= R286), D313 (= D310), H337 (= H334), S339 (= S336), G340 (= G337), S385 (= S382)

8bxxAA Formate dehydrogenase
71% identity, 96% coverage: 2:372/386 of query aligns to 1:370/370 of 8bxxAA

query
sites
8bxxAA

A

A

K

K

I

I

L

L

C

C

A

V

L

L

F

Y

P

D

D

D

P

P

V

I

T

T

G

G

Y

Y

P

P

P

K

S

S

Y

Y

A

A

R

R

T

A

D

D

V

V

P

P

S

K

I

I

A

D

S

H

Y

Y

P

P

G

G

Q

Q

R

T

P

A

P

P

S

T

P

P

K

K

G

Q

P

-

L

I

G

D

F

F

K

T

P

P

G

G

E

E

L

L

V

L

G

G

S

S

V

V

S

S

G

G

E

E

L

L

G

G

L

L

R

R

H

K

Y

Y

L

L

E

E

S

G

Q

L

G

G

H

H

E

T

L

L

I

V

V

V

T

T

S

S

D

D

K

K

D

E

G

G

P

E

D

D

S

S

I

V

F

F

E

E

R

R

H

E

L

L

C

P

D

D

A

A

D

E

I

I

V

V

I

I

S

S

Q

Q

P
|
P

F
|
F

W

W

P

P

A

A

Y

Y

L

L

T

T

R

P

E

E

R

R

I

I

A

A

K

K

A

A

K

K

L

L

K

K

L

L

A

A

L

V

T

T

A

A

G

G

I
|
I

G

G

S

S

D

D

H

H

V

V

D

D

L

L

Q

E

A

A

A

I

E

K

R

N

D

G

I

I

T

T

V

V

A

A

E

E

V

V

T

T

F

Y

S

S

N
|
N

S

S

I

I

S

S

V
|
V

A

S

E

E

H

H

V

V

M

M

T

M

V

I

L

L

A

S

L

L

V

V

R

R

N

N

Y

Y

L

I

P

P

S

S

H

Y

Q

Q

Y

W

A

V

V

I

N

K

G

G

W

W

N

N

I

I

A

A

D

D

C

C

V

V

A

E

R

R

S

S

Y

Y

D

D

L

L

E

E

G

A

M

M

H

H

F

V

G

G

T

T

I

V

G
x
A

A

A

G
|
G

R
|
R

I
|
I

G

G

L

L

A

A

V

V

L

L

R

K

R

R

L

L

K

K

P

P

F

F

D

D

V

V

H

K

L

L

H

H

Y

Y

Y
x
F

D
|
D

P
x
Q

H

H

R

R

L

L

S

P

P

E

E

S

L

V

E

E

R

N

E

E

L

L

N

G

V

L

T

T

F

Y

H

H

S

P

S

S

A

V

E

E

S

D

L

M

V

V

R

K

V

V

C

C

D

D

V

V

I

V

N

T

L

I

Q

N

S

A

P
|
P

L

L

Y
x
H

P

P

S

G

T

T

E

L

H

D

M

L

F

F

N

N

D

D

A

E

M

L

L

I

A

S

Q

K

I

M

K

K

P

R

G

G

A

A

Y

Y

L

L

I

V

N

N

C
x
T

A
|
A

R
|
R

G

G

K

K

L

I

C

C

D

N

A

R

D

D

A

A

V

V

A

V

R

R

A

A

L

L

Q

E

S

S

G

G

R

Q

L

L

A

A

G

G

Y

Y

G

A

G

G

D
|
D

V

V

W

W

F

F

P

P

Q

Q

P

P

A

A

P

P

A

K

E

D

H

H

P

P

W

W

R

R

S

T

M

M

P

P

N

H

E

H

G

G

M

M

T

T

P

P

H
|
H

I

I

S
|
S

G
|
G

A

T

S

S

L

L

S

S

A

A

Q

Q

A

A

R

R

Y

Y

A

A

G

G

T

T

R

R

E

E

I

I

L

L

E

E

C

C

F

W

L

F

E

E

G

E

R

R

A

P

I

I

R

R

A

E

E

E

Y

Y

L

L

I

I

V

V

N

D

G

G

binding azide ion: P97 (= P99), F98 (= F100), I122 (= I124), R284 (= R286), H332 (= H334)
binding nicotinamide-adenine-dinucleotide: I122 (= I124), N146 (= N148), V150 (= V152), A198 (≠ G200), G200 (= G202), R201 (= R203), I202 (= I204), F220 (≠ Y222), D221 (= D223), Q222 (≠ P224), P256 (= P258), H258 (≠ Y260), T282 (≠ C284), A283 (= A285), R284 (= R286), D308 (= D310), H332 (= H334), S334 (= S336), G335 (= G337)

8oq2A Binding of NADP to a formate dehydrogenase from starkeya novella.
71% identity, 98% coverage: 2:380/386 of query aligns to 2:379/381 of 8oq2A

query
sites
8oq2A

A

A

K

K

I

I

L

L

C

C

A

V

L

L

F

Y

P

D

D

D

P

P

V

V

T

D

G

G

Y

Y

P

P

P

K

S

T

Y

Y

A

A

R

R

T

D

D

D

V

L

P

P

S

K

I

I

A

D

S

H

Y

Y

P

P

G

G

Q

Q

R

T

P

L

P

P

S

T

P

P

K

K

G

A

P

-

L

I

G

D

F

F

K

T

P

P

G

G

E

A

L

L

V

L

G

G

S

S

V

V

S

S

G

G

E

E

L

L

G

G

L

L

R

R

H

K

Y

Y

L

L

E

E

S

A

Q

N

G

G

H

H

E

T

L

F

I

V

V

V

T

T

S

S

D

D

K

K

D

D

G

G

P

P

D

D

S

S

I

V

F

F

E

E

R

R

H

E

L

L

C

V

D

D

A

A

D

D

I

V

V

V

I

I

S

S

Q

Q

P

P

F

F

W

W

P

P

A

A

Y

Y

L

L

T

T

R

P

E

E

R

R

I

I

A

A

K

K

A

A

K

K

K

N

L

L

K

K

L

L

A

A

L

L

T

T

A

A

G

G

I
|
I

G

G

S

S

D

D

H

H

V

V

D

D

L

L

Q

Q

A

S

A

A

A

I

E

D

R

R

D

G

I

I

T

T

V

V

A

A

E

E

V

V

T

T

F

Y

S

C

N
|
N

S

S

I

I

S

S

V
|
V

A

A

E

E

H

H

V

V

M

M

T

M

V

I

L

L

A

G

L

L

V

V

R

R

N

N

Y

Y

L

I

P

P

S

S

H

H

Q

D

Y

W

A

A

V

R

N

K

G

G

W

W

N

N

I

I

A

A

D

D

C

C

V

V

A

E

R

H

S

S

Y

Y

D

D

L

L

E

E

G

G

M

M

H

T

F

V

G

G

T

S

I

V

G

G

A

A

G
|
G

R
|
R

I
|
I

G

G

L

L

A

A

V

V

L

L

R

R

L

L

K

A

P

P

F

F

D

D

V

V

H

K

L

L

H

H

Y

Y

Y

T

D
x
Q

P
x
R

H
|
H

R

R

L

L

S

P

P

E

E

A

L

V

E

E

R

K

E

E

L

L

N

G

V

L

T

V

F

W

H

H

S

D

S

T

A

R

E

E

S

D

L

M

V

Y

R

P

V

H

C

C

D

D

V

V

I

V

N

T

L

L

Q

N

S
x
V

P
|
P

L

L

Y
x
H

P

P

S

E

T
|
T

E

E

H

H

M

M

F

I

N

N

D

D

A

E

M

T

L

L

A

K

Q

L

I

F

K

K

P

R

G

G

A

A

Y

Y

L

I

I

V

N

N

C
x
T

A
|
A

R
|
R

G

G

K

K

L

L

C

A

D

D

A

R

D

D

A

A

V

I

A

V

R

R

A

A

L

I

Q

E

S

S

G

G

R

Q

L

L

A

A

G

G

Y

Y

G

A

G

G

D
|
D

V

V

W

W

F

F

P

P

Q

Q

P

P

A

A

P

P

A

K

E

D

H

H

P

P

W

W

R

R

S

T

M

M

P

K

N

W

E

E

G

G

M

M

T

T

P

P

H
|
H

I

I

S
|
S

G
|
G

A

T

S

S

L

L

S

S

A

A

Q

Q

A

A

R

R

Y

Y

A

A

G

G

T

T

R

R

E

E

I

I

L

L

E

E

C

C

F

F

L

F

E

E

G

G

R

R

A

P

I

I

R

R

A

D

E

E

Y

Y

L

L

I

I

V

V

N

Q

G

G

K

A

L

L

A

A

G

G

T

T

G

G

A

A

binding nadp nicotinamide-adenine-dinucleotide phosphate: I123 (= I124), N147 (= N148), V151 (= V152), G201 (= G202), R202 (= R203), I203 (= I204), Q222 (≠ D223), R223 (≠ P224), H224 (= H225), V256 (≠ S257), P257 (= P258), H259 (≠ Y260), T262 (= T263), T283 (≠ C284), A284 (= A285), R285 (= R286), D309 (= D310), H333 (= H334), S335 (= S336), G336 (= G337)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 381

7ya4A Formate dehydrogenase from novosphingobium sp. Ap12 with NAD and azide
68% identity, 99% coverage: 2:385/386 of query aligns to 1:383/384 of 7ya4A

query
sites
7ya4A

A

A

K

K

I

I

L

L

C

C

A

V

L

L

F

Y

P

P

D

D

P

P

V

V

T

N

G

G

Y

Y

P

P

P

K

S

T

Y

Y

A

A

R

R

T

D

D

E

V

I

P

P

S

N

I

I

A

T

S

V

Y

Y

P

D

G

N

G

G

Q

Q

R

T

P

A

P

P

S

T

P

P

K

K

G

A

P

-

L

I

G

D

F

F

K

K

P

P

G

G

E

E

L

L

V

L

G

G

S

S

V

V

S

S

G

G

E

E

L

L

G

G

L

L

R

R

H

K

Y

Y

L

L

E

E

S

G

Q

L

G

G

H

H

E

E

L

F

I

I

V

V

T

T

S

S

D

D

K

K

D

E

G

G

P

P

D

D

S

S

I

E

F

F

E

E

R

K

H

H

L

L

C

P

D

D

A

A

D

E

I

I

V

I

I

I

S

S

Q

Q

P
|
P

F
|
F

W

W

P

P

A

A

Y

Y

L

L

T

G

R

P

E

E

R

R

I

L

A

A

K

K

A

A

K

K

L

L

K

K

L

L

A

A

L

L

T

T

A

A

G

G

I
|
I

G

G

S

S

D

D

H

H

V

V

D

D

L

L

Q

E

A

S

A

A

A

I

E

K

R

H

D

G

I

V

T

T

V

V

A

A

E

E

V

V

T

T

F

G

S

S

N
|
N

S
|
S

I

I

S

S

V
|
V

A

S

E

E

H

H

V

A

V

V

M

M

T

M

V

I

L

L

A

S

L

L

V

V

R

R

N

N

Y

Y

L

I

P

P

S

A

H

H

Q

E

Y

W

A

A

V

E

N

K

G

G

W

W

N

N

I

I

A

A

D

D

C

C

V

V

A

E

R

R

S

S

Y

Y

D

D

L

V

E

E

G

G

M

M

H

H

F

V

G

G

T

T

I

V

G
x
A

A

A

G
|
G

R
|
R

I
|
I

G

G

L

L

A

A

V

I

L

L

R

K

R

R

L

M

K

K

P

P

F

F

D

D

V

V

H

H

L

L

H

H

Y

Y

Y
|
Y

D

A

P
x
R

H

H

R

R

L

L

S

S

P

K

E

E

L

E

E

E

R

E

E

E

L

L

N

G

V

L

T

T

F

F

H

H

S

E

S

N

A

V

E

E

S

D

L

M

V

V

R

K

V

V

C

C

D

D

V

V

I

V

N

T

L

I

Q

N

S

A

P
|
P

L

L

Y
x
H

P

P

S
x
E

T
|
T

E

H

H

H

M

M

F

F

N

D

D

E

A

A

M

M

L

I

A

K

Q

K

I

M

K

K

P

R

G

G

A

A

Y

Y

L

I

I

V

N

N

C
x
T

A
|
A

R
|
R

G

A

K

E

L

I

C

C

D

D

A

R

D

D

A

A

V

I

A

V

R

R

A

A

L

V

Q

E

S

S

G

G

R

H

L

L

A

A

G

G

Y

Y

G

A

G

G

D
|
D

V

V

W

W

F

N

P

P

Q

Q

P

P

A

A

P

P

A

A

E

D

H

H

P

P

W

W

R

R

S

T

M

M

P

P

N

W

E

N

G

G

M

M

T

T

P

P

H
|
H

I

M

S
|
S

G
|
G

A

T

S

S

L

L

S

S

A

G

Q

Q

A

A

R

R

Y

Y

A

T

A

A

G

G

T

T

R

R

E

E

I

I

L

L

E

E

C

C

F

W

L

F

E

E

G

G

R

R

A

P

I

I

R

R

A

E

E

D

Y

Y

L

V

I

I

V

V

N

D

G

G

K

K

L

L

A

A

G

G

T

T

G

G

A

A

A

L

S
|
S

Y

Y

K

T

L

V

binding azide ion: P97 (= P99), F98 (= F100), I122 (= I124), R284 (= R286), H332 (= H334)
binding nicotinamide-adenine-dinucleotide: I122 (= I124), N146 (= N148), S147 (= S149), V150 (= V152), A198 (≠ G200), G200 (= G202), R201 (= R203), I202 (= I204), Y220 (= Y222), R222 (≠ P224), P256 (= P258), H258 (≠ Y260), E260 (≠ S262), T261 (= T263), T282 (≠ C284), A283 (= A285), R284 (= R286), D308 (= D310), H332 (= H334), S334 (= S336), G335 (= G337), S380 (= S382)

7ya3B Formate dehydrogenase from novosphingobium sp. Ap12 with NADP and azide
68% identity, 99% coverage: 2:385/386 of query aligns to 1:383/384 of 7ya3B

query
sites
7ya3B

A

A

K

K

I

I

L

L

C

C

A

V

L

L

F

Y

P

P

D

D

P

P

V

V

T

N

G

G

Y

Y

P

P

P

K

S

T

Y

Y

A

A

R

R

T

D

D

E

V

I

P

P

S

N

I

I

A

T

S

V

Y

Y

P

D

G

N

G

G

Q

Q

R

T

P

A

P

P

S

T

P

P

K

K

G

A

P

-

L

I

G

D

F

F

K

K

P

P

G

G

E

E

L

L

V

L

G

G

S

S

V

V

S

S

G

G

E

E

L

L

G

G

L

L

R

R

H

K

Y

Y

L

L

E

E

S

G

Q

L

G

G

H

H

E

E

L

F

I

I

V

V

T

T

S

S

D

D

K

K

D

E

G

G

P

P

D

D

S

S

I

E

F

F

E

E

R

K

H

H

L

L

C

P

D

D

A

A

D

E

I

I

V

I

I

I

S

S

Q

Q

P

P

F

F

W

W

P

P

A

A

Y

Y

L

L

T

G

R

P

E

E

R

R

I

L

A

A

K

K

A

A

K

K

L

L

K

K

L

L

A

A

L

L

T

T

A

A

G

G

I
|
I

G

G

S

S

D

D

H

H

V

V

D

D

L

L

Q

E

A

S

A

A

A

I

E

K

R

H

D

G

I

V

T

T

V

V

A

A

E

E

V

V

T

T

F

G

S

S

N
|
N

S

S

I

I

S

S

V
|
V

A

S

E

E

H

H

V

A

V

V

M

M

T

M

V

I

L

L

A

S

L

L

V

V

R

R

N

N

Y

Y

L

I

P

P

S

A

H

H

Q

E

Y

W

A

A

V

E

N

K

G

G

W

W

N

N

I

I

A

A

D

D

C

C

V

V

A

E

R

R

S

S

Y

Y

D

D

L

V

E

E

G

G

M

M

H

H

F

V

G

G

T

T

I

V

G
x
A

A

A

G
|
G

R
|
R

I
|
I

G

G

L

L

A

A

V

I

L

L

R

K

R

R

L

M

K

K

P

P

F

F

D

D

V

V

H

H

L

L

H

H

Y

Y

D

A

P
x
R

H
|
H

R

R

L

L

S

S

P

K

E

E

L

E

E

E

R

E

E

E

L

L

N

G

V

L

T

T

F

F

H

H

S

E

S

N

A

V

E

E

S

D

L

M

V

V

R

K

V

V

C

C

D

D

V

V

I

V

N

T

L

I

Q

N

S

A

P
|
P

L

L

Y
x
H

P

P

S
x
E

T

T

E

H

H

H

M

M

F

F

N

D

D

E

A

A

M

M

L

I

A

K

Q

K

I

M

K

K

P

R

G

G

A

A

Y

Y

L

I

I

V

N

N

C
x
T

A
|
A

R
|
R

G

A

K

E

L

I

C

C

D

D

A

R

D

D

A

A

V

I

A

V

R

R

A

A

L

V

Q

E

S

S

G

G

R

H

L

L

A

A

G

G

Y

Y

G

A

G

G

D
|
D

V

V

W

W

F

N

P

P

Q

Q

P

P

A

A

P

P

A

A

E

D

H

H

P

P

W

W

R

R

S

T

M

M

P

P

N

W

E

N

G

G

M

M

T

T

P

P

H
|
H

I

M

S
|
S

G
|
G

A

T

S

S

L

L

S

S

A

G

Q

Q

A

A

R

R

Y

Y

A

T

A

A

G

G

T

T

R

R

E

E

I

I

L

L

E

E

C

C

F

W

L

F

E

E

G

G

R

R

A

P

I

I

R

R

A

E

E

D

Y

Y

L

V

I

I

V

V

N

D

G

G

K

K

L

L

A

A

G

G

T

T

G

G

A

A

A

L

S
|
S

Y

Y

K

T

L

V

binding azide ion: I122 (= I124), R284 (= R286), H332 (= H334)
binding nadp nicotinamide-adenine-dinucleotide phosphate: I122 (= I124), N146 (= N148), V150 (= V152), A198 (≠ G200), G200 (= G202), R201 (= R203), I202 (= I204), R222 (≠ P224), H223 (= H225), P256 (= P258), H258 (≠ Y260), E260 (≠ S262), T282 (≠ C284), A283 (= A285), R284 (= R286), D308 (= D310), H332 (= H334), S334 (= S336), G335 (= G337), S380 (= S382)

4xyeA Granulicella m. Formate dehydrogenase (fdh) in complex with NAD(+) (see paper)
69% identity, 100% coverage: 2:386/386 of query aligns to 1:384/384 of 4xyeA

query
sites
4xyeA

A

A

K

K

I

V

L

L

C

C

A

V

L

L

F

Y

P

D

D

D

P

P

V

T

T

S

G

G

Y

Y

P

P

S

L

Y

Y

A

A

R

R

T

N

D

A

V

I

P

P

S

K

I

I

A

E

S

R

Y

Y

P

P

G

D

G

G

Q

Q

R

T

P

V

P

P

S

N

P

P

K

K

G

H

P

-

L

I

G

D

F

F

K

V

P

P

G

G

E

E

L

L

V

L

G

G

S

C

V

V

S

S

G

G

E

E

L

L

G

G

L

L

R

R

H

S

Y

Y

L

L

E

E

S

D

Q

L

G

G

H

H

E

T

L

F

I

I

V

V

T

T

S

S

D

D

K

K

D

E

G

G

P

P

D

N

S

S

I

V

F

F

E

E

R

K

H

E

L

L

C

P

D

D

A

A

D

D

I

I

V

V

I

I

S

S

Q

Q

P

P

F
|
F

W

W

P

P

A

A

Y

Y

L

L

T

T

R

A

E

E

R

R

I

I

A

A

K

K

A

A

K

K

L

L

K

K

L

L

A

A

L

L

T

T

A

A

G

G

I
|
I

G

G

S

S

D

D

H

H

V

V

D

D

L

L

Q

N

A

A

A

I

E

K

R

A

D

G

I

I

T

T

V

V

A

A

E

E

V

E

T

T

F

F

S

S

N
|
N

S

G

I

I

S

C

V
|
V

A

A

E

E

H

H

V

A

V

V

M

M

T

M

V

I

L

L

A

A

L

L

V

V

R

R

N

N

Y

Y

L

L

P

P

S

S

H

H

Q

K

Y

I

A

A

V

E

N

E

G

G

W

W

N

N

I

I

A

A

D

D

C

C

V

V

A

S

R

R

S

S

Y

Y

D

D

L

L

E

E

G

G

M

M

H

H

F

V

G

G

T

T

I

V

G
x
A

A

A

G
|
G

R
|
R

I
|
I

G

G

L

L

A

A

V

V

L

L

R

R

L

L

K

K

P

P

F

F

D

D

V

V

H

K

L

L

H

H

Y

Y

Y

T

D
x
A

P
x
R

H

H

R

R

L

S

S

P

P

R

E

A

L

I

E

E

R

D

E

E

L

L

N

G

V

L

T

T

F

Y

H

H

S

A

S

T

A

A

E

E

S

E

L

M

V

A

R

E

V

V

C

C

D

D

V

V

I

I

N

S

L

I

Q

H

S
x
A

P
|
P

L

L

Y
|
Y

P

P

S

A

T

T

E

E

H

H

M

L

F

F

N

N

D

A

A

K

M

V

L

L

A

N

Q

K

I

M

K

R

P

H

G

G

A

S

Y

Y

L

L

I

V

N

N

C

T

A

A

R
|
R

G

A

K

E

L

I

C

C

D

D

A

R

D

D

A

D

V

I

A

V

R

R

A

A

L

L

Q

E

S

S

G

G

R

Q

L

L

A

A

G

G

Y

Y

G

A

G

G

D
|
D

V

V

W

W

F

F

P

P

Q
|
Q

P

P

A

A

P

P

A

A

E

N

H

H

P

P

W

W

R

R

S

N

M

M

P

P

N

H

E

N

G

G

M

M

T

T

P

P

H
|
H

I

M

S

S

G
|
G

A

S

S

S

L

L

S

S

A

G

Q

Q

A

A

R

R

Y

Y

A

A

G

G

T

T

R

R

E

E

I

I

L

L

E

E

C

C

F

W

L

F

E

E

G

N

R

R

A

P

I

I

R

R

A

D

E

E

Y

Y

L

L

I

I

V

V

N

S

G

N

G

G

K

K

L

L

A

A

G

G

T

T

G

G

A

A

A

K

S
|
S

Y

Y

K

G

L

V

G

G

active site: N146 (= N148), R284 (= R286), D308 (= D310), Q313 (= Q315), H332 (= H334)
binding nicotinamide-adenine-dinucleotide: F98 (= F100), I122 (= I124), N146 (= N148), V150 (= V152), A198 (≠ G200), G200 (= G202), R201 (= R203), I202 (= I204), A221 (≠ D223), R222 (≠ P224), A255 (≠ S257), P256 (= P258), Y258 (= Y260), R284 (= R286), H332 (= H334), G335 (= G337), S380 (= S382)

4xybA Granulicella m. Formate dehydrogenase (fdh) in complex with NADP(+) and nan3 (see paper)
69% identity, 100% coverage: 2:386/386 of query aligns to 1:384/384 of 4xybA

query
sites
4xybA

A

A

K

K

I

V

L

L

C

C

A

V

L

L

F

Y

P

D

D

D

P

P

V

T

T

S

G

G

Y

Y

P

P

S

L

Y

Y

A

A

R

R

T

N

D

A

V

I

P

P

S

K

I

I

A

E

S

R

Y

Y

P

P

G

D

G

G

Q

Q

R

T

P

V

P

P

S

N

P

P

K

K

G

H

P

-

L

I

G

D

F

F

K

V

P

P

G

G

E

E

L

L

V

L

G

G

S

C

V

V

S

S

G

G

E

E

L

L

G

G

L

L

R

R

H

S

Y

Y

L

L

E

E

S

D

Q

L

G

G

H

H

E

T

L

F

I

I

V

V

T

T

S

S

D

D

K

K

D

E

G

G

P

P

D

N

S

S

I

V

F

F

E

E

R

K

H

E

L

L

C

P

D

D

A

A

D

D

I

I

V

V

I

I

S

S

Q

Q

P
|
P

F
|
F

W

W

P

P

A

A

Y

Y

L

L

T

T

R

A

E

E

R

R

I

I

A

A

K

K

A

A

K

K

L

L

K

K

L

L

A

A

L

L

T

T

A

A

G

G

I
|
I

G

G

S

S

D

D

H

H

V

V

D

D

L

L

Q

N

A

A

A

I

E

K

R

A

D

G

I

I

T

T

V

V

A

A

E

E

V

E

T

T

F

F

S

S

N
|
N

S

G

I

I

S

C

V
|
V

A

A

E

E

H

H

V

A

V

V

M

M

T

M

V

I

L

L

A

A

L

L

V

V

R

R

N

N

Y

Y

L

L

P

P

S

S

H

H

Q

K

Y

I

A

A

V

E

N

E

G

G

W

W

N

N

I

I

A

A

D

D

C

C

V

V

A

S

R

R

S

S

Y

Y

D

D

L

L

E

E

G

G

M

M

H

H

F

V

G

G

T

T

I

V

G
x
A

A

A

G
|
G

R
|
R

I
|
I

G

G

L

L

A

A

V

V

L

L

R

R

L

L

K

K

P

P

F

F

D

D

V

V

H

K

L

L

H

H

Y

Y

Y

T

D

A

P
x
R

H
|
H

R

R

L

S

S

P

P

R

E

A

L

I

E

E

R

D

E

E

L

L

N

G

V

L

T

T

F

Y

H

H

S

A

S

T

A

A

E

E

S

E

L

M

V

A

R

E

V

V

C

C

D

D

V

V

I

I

N

S

L

I

Q

H

S

A

P
|
P

L

L

Y
|
Y

P

P

S

A

T
|
T

E

E

H

H

M

L

F

F

N

N

D

A

A

K

M

V

L

L

A

N

Q

K

I

M

K

R

P

H

G

G

A

S

Y

Y

L

L

I

V

N

N

C
x
T

A
|
A

R
|
R

G

A

K

E

L

I

C

C

D

D

A

R

D

D

A

D

V

I

A

V

R

R

A

A

L

L

Q

E

S

S

G

G

R

Q

L

L

A

A

G

G

Y

Y

G

A

G

G

D
|
D

V

V

W

W

F

F

P

P

Q
|
Q

P

P

A

A

P

P

A

A

E

N

H

H

P

P

W

W

R

R

S

N

M

M

P

P

N

H

E

N

G

G

M

M

T

T

P

P

H
|
H

I

M

S
|
S

G
|
G

A

S

S

S

L

L

S

S

A

G

Q

Q

A

A

R

R

Y

Y

A

A

G

G

T

T

R

R

E

E

I

I

L

L

E

E

C

C

F

W

L

F

E

E

G

N

R

R

A

P

I

I

R

R

A

D

E

E

Y

Y

L

L

I

I

V

V

N

S

G

N

G

G

K

K

L

L

A

A

G

G

T

T

G

G

A

A

A

K

S
|
S

Y

Y

K

G

L

V

G

G

active site: N146 (= N148), R284 (= R286), D308 (= D310), Q313 (= Q315), H332 (= H334)
binding azide ion: P97 (= P99), F98 (= F100), I122 (= I124), R284 (= R286), H332 (= H334)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: I122 (= I124), N146 (= N148), V150 (= V152), A198 (≠ G200), G200 (= G202), R201 (= R203), I202 (= I204), R222 (≠ P224), H223 (= H225), P256 (= P258), Y258 (= Y260), T261 (= T263), T282 (≠ C284), A283 (= A285), R284 (= R286), D308 (= D310), H332 (= H334), S334 (= S336), G335 (= G337), S380 (= S382)

7arzA Ternary complex of NAD-dependent formate dehydrogenase from physcomitrium patens
50% identity, 85% coverage: 50:376/386 of query aligns to 30:357/361 of 7arzA

query
sites
7arzA

E

E

L

F

V

L

G

G

S

C

V

V

S

E

G

N

E

A

L

L

G

G

L

I

R

R

H

E

Y

W

L

L

E

E

S

S

Q

K

G

G

H

H

E

K

L

Y

I

V

V

V

T

T

S

S

D

D

K

K

D

D

G

G

P

P

D

D

S

S

I

E

F

L

E

D

R

K

H

E

L

L

C

A

D

D

A

A

D

H

I

I

V

L

I

I

S
x
T

Q

T

P
|
P

F
|
F

W

H

P

P

A

A

Y

Y

L

M

T

T

R

K

E

E

R

R

I

L

A

A

K

K

A

A

K

K

K

N

L

L

K

E

L

L

A
x
L

L
x
V

T
|
T

A

A

G
|
G

I
x
V

G

G

S

S

D

D

H

H

V

I

D

D

L

L

Q

H

A

A

E

E

R

K

D

G

I

L

T

T

V

V

A

S

E

E

V

V

T

T

F

G

S

S

N
|
N

S

V

I

T

S

S

V
|
V

A

A

E

E

H

D

V

E

V

V

M

L

T

R

V

I

L

L

A

V

L

L

V

V

R

R

N

N

Y

F

L

A

P

P

S

G

H

W

Q

K

Y

Q

A

V

V

S

N

E

G

G

W

W

N

N

I

V

A

A

D

A

C

V

V

V

A

H

R

H

S

A

Y

Y

D

D

L

L

E

I

G

D

M

R

H

T

F

V

G

G

T

T

I

V

G

G

A

G

G
|
G

R
|
R

I
|
I

G

G

L

Q

A

E

V

L

L

M

R

K

R

R

L

L

K

K

P

G

F

F

D

G

V

L

-

K

H

E

L

M

H

L

Y

Y

D
|
D

P
x
R

H

N

R

S

L

L

S

G

P

A

E

E

L

R

E

E

R

K

E

E

L

L

N

G

V

C

T

K

F

R

H

E

S

T

S

D

A

L

E

D

S

T

L

M

V

L

R

S

V

K

C

C

D

D

V

V

I

V

N

V

L

V

Q

N

S
x
T

P
|
P

L

L

Y

T

P

D

S
x
Q

T

T

E

R

H

G

M

L

F

F

N

N

D

K

A

E

M

R

L

I

A

A

Q

K

I

M

K

K

P

K

G

G

A

A

Y

Y

L

L

I

V

N

N

C
x
N

A
|
A

R
|
R

G

G

K

A

L

I

C

A

D

D

A

T

D

E

A

A

V

V

A

K

R

E

A

A

L

C

Q

E

S

S

G

G

R

H

L

L

A

G

G

G

Y

Y

G

G

D
|
D

V

V

W

W

F

N

P

A

Q

Q

P

P

A

A

P

G

A

K

E

D

H

H

P

P

W

W

R

R

S

Y

M

M

P

P

N

N

E

H

G

A

M

M

T

T

P

P

H
|
H

I

I

S
|
S

G
|
G

A

T

S

T

L

L

S

D

A

A

Q

Q

A

K

R

R

Y

F

A

A

G

G

T

T

R

K

E

D

I

M

L

I

E

D

C

R

F

W

L

L

E

K

G

H

R

E

A

A

I

F

R

P

A

E

E

Q

Y

N

L

Y

I

I

V

V

N

R

G

E

G

G

K

K

L

L

A

A

binding azide ion: T77 (≠ S97), P79 (= P99), F80 (= F100), L99 (≠ A119), V100 (≠ L120), T101 (= T121), G103 (= G123), V104 (≠ I124), R267 (= R286), H315 (= H334)
binding nicotinamide-adenine-dinucleotide: V104 (≠ I124), N128 (= N148), V132 (= V152), G182 (= G202), R183 (= R203), I184 (= I204), D204 (= D223), R205 (≠ P224), T238 (≠ S257), P239 (= P258), Q243 (≠ S262), N265 (≠ C284), A266 (= A285), R267 (= R286), D291 (= D310), H315 (= H334), S317 (= S336), G318 (= G337)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 359

6t8zAAA Formate dehydrogenase (see paper)
54% identity, 85% coverage: 48:376/386 of query aligns to 24:360/373 of 6t8zAAA

query
sites
6t8zAAA

P

P

G

G

E

-

L

L

V

L

G

G

S

T

V

T

S

E

G

N

E

E

L

L

G

G

L

L

R

R

H

K

Y

W

L

L

E

E

S

D

Q

Q

G

G

H

H

E

T

L

L

I

V

V

T

T

T

S

S

D

D

K

K

D

D

G

R

P

E

D

G

S

S

I

T

F

F

E

D

R

R

H

E

L

L

C

E

D

D

A

A

D

E

I

I

V

I

I

I

S

T

Q

T

P

P

F

F

W

H

P

P

A

G

Y

Y

L

L

T

T

R

A

E

E

R

R

I

L

A

A

K

R

A

A

K

K

L

L

K

K

L

L

A

A

L

V

T

T

A

A

G

G

I
|
I

G

G

S

S

D

D

H

H

V

V

D

D

L

L

Q

D

A

A

A

N

E

K

R

T

D

N

-

G

I

I

T

T

V

V

A

A

E

E

V

V

T

T

F

G

S

S

N
|
N

S

V

I

V

S

S

V
|
V

A

A

E

E

H

H

V

V

M

M

T

T

V

I

L

L

A

V

L

L

V

V

R

R

N

N

Y

F

L

V

P

P

S

A

H

H

Q

E

Y

Q

A

I

V

E

N

A

G

G

G

R

W

W

N

D

I

V

A

A

D

E

C

V

V

A

A

K

R

D

S

E

Y

Y

D

D

L

L

E

E

G

G

M

K

H

V

F

V

G

G

T

T

I

V

G
|
G

A

V

G
|
G

R
|
R

I
|
I

G

G

L

E

A

R

V

V

L

L

R

R

L

L

K

K

P

G

F

F

D

D

V

C

-

K

H

E

L

L

H

L

Y

Y

Y
|
Y

D
|
D

P
x
Y

H
x
Q

R

P

L

L

S

S

P

P

E

E

L

K

E

E

R

K

E

E

L

I

N

G

V

C

T

R

F

R

H

V

S

E

S

N

A

L

E

E

S

E

L

M

V

L

R

A

V

Q

C

C

D

D

V

V

I

V

N

T

L

I

Q

N

S
x
C

P
|
P

L

L

Y
x
H

P

E

S

S

T

T

E

R

H

G

M

L

F

F

N

N

D

K

A

D

M

L

L

I

A

S

Q

K

I

M

K

K

P

R

G

G

A

S

Y

W

L

L

I

V

N

N

C
x
T

A
|
A

R
|
R

G

G

K

A

L

I

C

V

D

V

A

K

D

E

A

D

V

V

A

A

R

E

A

A

L

L

Q

R

S

T

G

G

R

H

L

L

A

R

G

G

Y

Y

G

G

D
|
D

V

V

W

W

F

F

P

P

Q

Q

P

P

A

A

P

P

A

A

E

D

H

H

P

V

W

L

R

R

S

T

M

A

P

K

N

N

-

P

-

F

-

G

E

N

G

A

M

M

T

V

P

P

H
|
H

I

M

S
|
S

G
|
G

A

T

S

S

L

L

S

D

A

A

Q

Q

A

K

R

R

Y

Y

A

A

A

E

G

G

T

V

R

K

E

R

I

I

L

L

E

D

C

S

F

Y

L

L

E

S

G

G

R

R

-

F

A

D

I

Y

R

R

A

P

E

E

Y

D

L

L

I

I

V

V

N

H

G

Q

G

G

K

K

L

Y

A

A

binding nicotinamide-adenine-dinucleotide: I99 (= I124), N125 (= N148), V129 (= V152), G177 (= G200), G179 (= G202), R180 (= R203), I181 (= I204), Y200 (= Y222), D201 (= D223), Y202 (≠ P224), Q203 (≠ H225), C235 (≠ S257), P236 (= P258), H238 (≠ Y260), T262 (≠ C284), A263 (= A285), R264 (= R286), D288 (= D310), H317 (= H334), S319 (= S336), G320 (= G337)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 363

6t8yBBB Formate dehydrogenase (see paper)
54% identity, 85% coverage: 48:376/386 of query aligns to 25:361/375 of 6t8yBBB

query
sites
6t8yBBB

P

P

G

G

E

-

L

L

V

L

G

G

S

T

V

T

S

E

G

N

E

E

L

L

G

G

L

L

R

R

H

K

Y

W

L

L

E

E

S

D

Q

Q

G

G

H

H

E

T

L

L

I

V

V

T

T

T

S

S

D

D

K

K

D

D

G

R

P

E

D

G

S

S

I

T

F

F

E

D

R

R

H

E

L

L

C

E

D

D

A

A

D

E

I

I

V

I

I

I

S

T

Q

T

P

P

F

F

W

H

P

P

A

G

Y

Y

L

L

T

T

R

A

E

E

R

R

I

L

A

A

K

R

A

A

K

K

L

L

K

K

L

L

A

A

L

V

T

T

A

A

G

G

I
|
I

G

G

S

S

D

D

H

H

V

V

D

D

L

L

Q

D

A

A

A

N

E

K

R

T

D

N

-

G

I

I

T

T

V

V

A

A

E

E

V

V

T

T

F

G

S

S

N
|
N

S

V

I

V

S

S

V
|
V

A

A

E

E

H

H

V

V

M

M

T

T

V

I

L

L

A

V

L

L

V

V

R

R

N

N

Y

F

L

V

P

P

S

A

H

H

Q

E

Y

Q

A

I

V

E

N

A

G

G

G

R

W

W

N

D

I

V

A

A

D

E

C

V

V

A

A

K

R

D

S

E

Y

Y

D

D

L

L

E

E

G

G

M

K

H

V

F

V

G

G

T

T

I

V

G
|
G

A

V

G
|
G

R
|
R

I
|
I

G

G

L

E

A

R

V

V

L

L

R

R

L

L

K

K

P

G

F

F

D

D

V

C

-

K

H

E

L

L

H

L

Y

Y

Y
|
Y

D
|
D

P
x
Y

H
x
Q

R

P

L

L

S

S

P

P

E

E

L

K

E

E

R

K

E

E

L

I

N

G

V

C

T

R

F

R

H

V

S

E

S

N

A

L

E

E

S

E

L

M

V

L

R

A

V

Q

C

C

D

D

V

V

I

V

N

T

L

I

Q

N

S
x
C

P
|
P

L

L

Y
x
H

P

E

S

S

T

T

E

R

H

G

M

L

F

F

N

N

D

K

A

D

M

L

L

I

A

S

Q

K

I

M

K

K

P

R

G

G

A

S

Y

W

L

L

I

V

N

N

C
x
T

A
|
A

R
|
R

G

G

K

A

L

I

C

V

D

V

A

K

D

E

A

D

V

V

A

A

R

E

A

A

L

L

Q

R

S

T

G

G

R

H

L

L

A

R

G

G

Y

Y

G

G

D
|
D

V

V

W

W

F

F

P

P

Q

Q

P

P

A

A

P

P

A

A

E

D

H

H

P

V

W

L

R

R

S

T

M

A

P

K

N

N

-

P

-

F

-

G

E

N

G

A

M

M

T

V

P

P

H
|
H

I

M

S
|
S

G
|
G

A

T

S

S

L

L

S

D

A

A

Q

Q

A

K

R

R

Y

Y

A

A

A

E

G

G

T

V

R

K

E

R

I

I

L

L

E

D

C

S

F

Y

L

L

E

S

G

G

R

R

-

F

A

D

I

Y

R

R

A

P

E

E

Y

D

L

L

I

I

V

V

N

H

G

Q

G

G

K

K

L

Y

A

A

binding 1,4-dihydronicotinamide adenine dinucleotide: I100 (= I124), N126 (= N148), V130 (= V152), G178 (= G200), G180 (= G202), R181 (= R203), I182 (= I204), Y201 (= Y222), D202 (= D223), Y203 (≠ P224), Q204 (≠ H225), C236 (≠ S257), P237 (= P258), H239 (≠ Y260), T263 (≠ C284), A264 (= A285), R265 (= R286), D289 (= D310), H318 (= H334), S320 (= S336), G321 (= G337)

Sites not aligning to the query:

binding 1,4-dihydronicotinamide adenine dinucleotide: 364

3n7uA NAD-dependent formate dehydrogenase from higher-plant arabidopsis thaliana in complex with NAD and azide
52% identity, 84% coverage: 51:376/386 of query aligns to 22:347/351 of 3n7uA

query
sites
3n7uA

L

F

V

L

G

G

S

C

V

V

S

E

G

N

E

A

L

L

G

G

L

I

R

R

H

D

Y

W

L

L

E

E

S

S

Q

Q

G

G

H

H

E

Q

L

Y

I

I

V

V

T

T

S

D

D

D

K

K

D

E

G

G

P

P

D

D

S

C

I

E

F

L

E

E

R

K

H

H

L

I

C

P

D

D

A

L

D

H

I

V

V

L

I

I

S

S

Q

T

P
|
P

F
|
F

W

H

P

P

A

A

Y

Y

L

V

T

T

R

A

E

E

R

R

I

I

A

K

K

K

A

A

K

K

K

N

L

L

K

K

L

L

A

L

L

L

T

T

A

A

G

G

I
|
I

G

G

S

S

D

D

H

H

V

I

D

D

L

L

Q

Q

A

A

E

A

R

A

D

G

I

L

T

T

V

V

A

A

E

E

V

V

T

T

F

G

S

S

N
|
N

S

V

I

V

S

S

V
|
V

A

A

E

E

H

D

V

E

V

L

M

M

T

R

V

I

L

L

A

I

L

L

V

M

R

R

N

N

Y

F

L

V

P

P

S

G

H

Y

Q

N

Y

Q

A

V

V

V

N

K

G

G

G

E

W

W

N

N

I

V

A

A

D

G

C

I

V

A

A

Y

R

R

S

A

Y

Y

D

D

L

L

E

E

G

G

M

K

H

T

F

I

G

G

T

T

I

V

G

G

A

A

G
|
G

R
|
R

I
|
I

G

G

L

K

A

L

V

L

L

L

R

Q

R

R

L

L

K

K

P

P

F

F

D

G

V

C

H

N

L

L

H

L

Y

Y

Y

H

D
|
D

P
x
R

H

L

R

Q

L

M

S

A

P

P

E

E

L

L

E

E

R

K

E

E

L

T

N

G

V

A

T

K

F

F

H

V

S

E

S

D

A

L

E

N

S

E

L

M

V

L

R

P

V

K

C

C

D

D

V

V

I

I

N

V

L

I

Q

N

S
x
M

P
|
P

L

L

Y

T

P

E

S

K

T

T

E

R

H

G

M

M

F

F

N

N

D

K

A

E

M

L

L

I

A

G

Q

K

I

L

K

K

P

K

G

G

A

V

Y

L

L

I

I

V

N

N

C
x
N

A
|
A

R
|
R

G

G

K

A

L

I

C

M

D

E

A

R

D

Q

A

A

V

V

A

V

R

D

A

A

L

V

Q

E

S

S

G

G

R

H

L

I

A

G

G

G

Y

Y

G

S

G

G

D
|
D

V

V

W

W

F

D

P

P

Q
|
Q

P

P

A

A

P

P

A

K

E

D

H

H

P

P

W

W

R

R

S

Y

M

M

P

P

N

N

E

Q

G

A

M

M

T

T

P

P

H
|
H

I

T

S
|
S

G
|
G

A

T

S

T

L

I

S

D

A

A

Q

Q

A

L

R

R

Y

Y

A

A

G

G

T

T

R

K

E

D

I

M

L

L

E

E

C

R

F

Y

L

F

E

K

G

G

R

E

A

D

I

F

R

P

A

T

E

E

Y

N

L

Y

I

I

V

V

N

K

G

D

G

G

K

E

L

L

A

A

active site: N119 (= N148), R257 (= R286), D281 (= D310), Q286 (= Q315), H305 (= H334)
binding azide ion: P70 (= P99), F71 (= F100), I95 (= I124), R257 (= R286), H305 (= H334)
binding nicotinamide-adenine-dinucleotide: I95 (= I124), N119 (= N148), V123 (= V152), G173 (= G202), R174 (= R203), I175 (= I204), D194 (= D223), R195 (≠ P224), M228 (≠ S257), P229 (= P258), N255 (≠ C284), A256 (= A285), R257 (= R286), D281 (= D310), H305 (= H334), S307 (= S336), G308 (= G337)

3jtmA Structure of recombinant formate dehydrogenase from arabidopsis thaliana
52% identity, 84% coverage: 51:376/386 of query aligns to 22:347/351 of 3jtmA

query
sites
3jtmA

L

F

V

L

G

G

S

C

V

V

S

E

G

N

E

A

L

L

G

G

L

I

R

R

H

D

Y

W

L

L

E

E

S

S

Q

Q

G

G

H

H

E

Q

L

Y

I

I

V

V

T

T

S

D

D

D

K

K

D

E

G

G

P

P

D

D

S

C

I

E

F

L

E

E

R

K

H

H

L

I

C

P

D

D

A

L

D

H

I

V

V

L

I

I

S

S

Q

T

P

P

F

F

W

H

P

P

A

A

Y

Y

L

V

T

T

R

A

E

E

R

R

I

I

A

K

K

K

A

A

K

K

K

N

L

L

K

K

L

L

A

L

L

L

T

T

A

A

G

G

I

I

G

G

S

S

D

D

H

H

V

I

D

D

L

L

Q

Q

A

A

E

A

R

A

D

G

I

L

T

T

V

V

A

A

E

E

V

V

T

T

F

G

S

S

N
|
N

S

V

I

V

S

S

V

V

A

A

E

E

H

D

V

E

V

L

M

M

T

R

V

I

L

L

A

I

L
|
L

V

M

R

R

N

N

Y

F

L

V

P

P

S

G

H

Y

Q

N

Y

Q

A

V

V

V

N

K

G

G

G

E

W

W

N

N

I

V

A

A

D

G

C

I

V

A

A

Y

R

R

S

A

Y

Y

D

D

L

L

E

E

G

G

M

K

H

T

F

I

G

G

T

T

I

V

G

G

A

A

G

G

R

R

I

I

G

G

L

K

A

L

V

L

L

L

R

Q

R

R

L

L

K

K

P

P

F

F

D

G

V

C

H

N

L

L

H

L

Y

Y

Y

H

D

D

P

R

H

L

R

Q

L

M

S

A

P

P

E

E

L

L

E

E

R

K

E

E

L

T

N

G

V

A

T

K

F

F

H

V

S

E

S

D

A

L

E

N

S

E

L

M

V

L

R

P

V

K

C

C

D

D

V

V

I

I

N

V

L

I

Q

N

S

M

P

P

L

L

Y

T

P

E

S

K

T

T

E

R

H

G

M

M

F

F

N

N

D

K

A

E

M

L

L

I

A

G

Q

K

I

L

K

K

P

K

G

G

A

V

Y

L

L

I

I

V

N

N

C

N

A

A

R
|
R

G

G

K

A

L

I

C

M

D

E

A

R

D

Q

A

A

V

V

A

V

R

D

A

A

L

V

Q

E

S

S

G

G

R

H

L

I

A
x
G

G

G

Y

Y

G

S

G

G

D
|
D

V

V

W

W

F

D

P

P

Q
|
Q

P

P

A

A

P

P

A

K

E

D

H

H

P

P

W

W

R

R

S

Y

M

M

P

P

N

N

E

Q

G

A

M

M

T

T

P

P

H
|
H

I

T

S

S

G

G

A

T

S

T

L

I

S

D

A

A

Q

Q

A

L

R

R

Y

Y

A

A

G

G

T

T

R

K

E

D

I

M

L

L

E

E

C

R

F

Y

L

F

E

K

G

G

R

E

A

D

I

F

R

P

A

T

E

E

Y

N

L

Y

I

I

V

V

N

K

G

D

G

G

K

E

L

L

A

A

active site: N119 (= N148), R257 (= R286), D281 (= D310), Q286 (= Q315), H305 (= H334)
binding azide ion: L134 (= L163), G276 (≠ A305)

Q9S7E4 Formate dehydrogenase, chloroplastic/mitochondrial; FDH; NAD-dependent formate dehydrogenase; EC 1.17.1.9 from Arabidopsis thaliana (Mouse-ear cress)
52% identity, 84% coverage: 51:376/386 of query aligns to 55:380/384 of Q9S7E4

query
sites
Q9S7E4

L

F

V

L

G

G

S

C

V

V

S

E

G

N

E

A

L

L

G

G

L

I

R

R

H

D

Y

W

L

L

E

E

S

S

Q

Q

G

G

H

H

E

Q

L

Y

I

I

V

V

T

T

S

D

D

D

K

K

D

E

G

G

P

P

D

D

S

C

I

E

F

L

E

E

R

K

H

H

L

I

C

P

D

D

A

L

D

H

I

V

V

L

I

I

S

S

Q

T

P

P

F

F

W

H

P

P

A

A

Y

Y

L

V

T

T

R

A

E

E

R

R

I

I

A

K

K

K

A

A

K

K

K

N

L

L

K

K

L

L

A

L

L

L

T

T

A

A

G

G

I

I

G

G

S

S

D

D

H

H

V

I

D

D

L

L

Q

Q

A

A

E

A

R

A

D

G

I

L

T

T

V

V

A

A

E

E

V

V

T

T

F

G

S

S

N

N

S

V

I

V

S

S

V

V

A

A

E

E

H

D

V

E

V

L

M

M

T

R

V

I

L

L

A

I

L

L

V

M

R

R

N

N

Y

F

L

V

P

P

S

G

H

Y

Q

N

Y

Q

A

V

V

V

N

K

G

G

G

E

W

W

N

N

I

V

A

A

D

G

C

I

V

A

A

Y

R

R

S

A

Y

Y

D

D

L

L

E

E

G

G

M

K

H

T

F

I

G

G

T

T

I

V

G

G

A

A

G

G

R
|
R

I
|
I

G

G

L

K

A

L

V

L

L

L

R

Q

R

R

L

L

K

K

P

P

F

F

D

G

V

C

H

N

L

L

H

L

Y

Y

Y

H

D
|
D

P

R

H

L

R

Q

L

M

S

A

P

P

E

E

L

L

E

E

R

K

E

E

L

T

N

G

V

A

T

K

F

F

H

V

S

E

S

D

A

L

E

N

S

E

L

M

V

L

R

P

V

K

C

C

D

D

V

V

I

I

N

V

L

I

Q

N

S

M

P
|
P

L
|
L

Y
x
T

P
x
E

S
x
K

T

T

E

R

H

G

M

M

F

F

N

N

D

K

A

E

M

L

L

I

A

G

Q

K

I

L

K

K

P

K

G

G

A

V

Y

L

L

I

I

V

N

N

C
x
N

A

A

R

R

G

G

K

A

L

I

C

M

D

E

A

R

D

Q

A

A

V

V

A

V

R

D

A

A

L

V

Q

E

S

S

G

G

R

H

L

I

A

G

G

G

Y

Y

G

S

G

G

D
|
D

V

V

W

W

F

D

P

P

Q

Q

P

P

A

A

P

P

A

K

E

D

H

H

P

P

W

W

R

R

S

Y

M

M

P

P

N

N

E

Q

G

A

M

M

T

T

P

P

H
|
H

I
x
T

S
|
S

G
|
G

A

T

S

T

L

I

S

D

A

A

Q

Q

A

L

R

R

Y

Y

A

A

G

G

T

T

R

K

E

D

I

M

L

L

E

E

C

R

F

Y

L

F

E

K

G

G

R

E

A

D

I

F

R

P

A

T

E

E

Y

N

L

Y

I

I

V

V

N

K

G

D

G

G

K

E

L

L

A

A

RI 207:208 (= RI 203:204) binding NAD(+)
D227 (= D223) binding NAD(+)
PLTEK 262:266 (≠ PLYPS 258:262) binding NAD(+)
N288 (≠ C284) binding NAD(+)
D314 (= D310) binding NAD(+)
HTSG 338:341 (≠ HISG 334:337) binding NAD(+)

Sites not aligning to the query:

1:29 modified: transit peptide, Chloroplast and mitochondrion

6d4bA Crystal structure of candida boidinii formate dehydrogenase v123a mutant complexed with NAD+ and azide
50% identity, 81% coverage: 50:361/386 of query aligns to 19:338/361 of 6d4bA

query
sites
6d4bA

E

K

L

L

V

Y

G

G

S

C

V

T

S

E

G

N

E

K

L

L

G

G

L

I

R

A

H

N

Y

W

L

L

E

K

S

D

Q

Q

G

G

H

H

E

E

L

L

I

I

V

T

T

T

S

S

D

D

K

K

D

E

G

G

P

G

D

N

S

S

I

V

F

L

E

D

R

Q

H

H

L

I

C

P

D

D

A

A

D

D

I

I

V

I

I

I

S

T

Q

T

P
|
P

F
|
F

W

H

P

P

A

A

Y

Y

L

I

T

T

R

K

E

E

R

R

I

I

A

D

K

K

A

A

K

K

L

L

K

K

L

L

A

V

L

V

T

V

A

A

G

G

I
x
V

G

G

S

S

D

D

H

H

V

I

D

D

L

L

Q

D

A

Y

A

I

A

N

E

Q

-

T

-

G

R

K

D

K

I

I

T

S

V

V

A

L

E

E

V

V

T

T

F

G

S

S

N
|
N

S

V

I

V

S

S

V

A

A

A

E

E

H

H

V

V

V

L

M

M

T

T

V

M

L

L

A

V

L

L

V

V

R

R

N

N

Y

F

L

V

P

P

S

A

H

H

Q

E

Y

Q

A

I

V

I

N

N

G

H

G

D

W

W

N

E

I

V

A

A

D

A

C

I

V

A

A

K

R

D

S

A

Y

Y

D

D

L

I

E

E

G

G

M

K

H

T

F

I

G

A

T

T

I

I

G

G

A

A

G
|
G

R
|
R

I
|
I

G

G

L

Y

A

R

V

V

L

L

R

E

R

R

L

L

K

V

P

P

F

F

D

N

-

P

V

K

H

E

L

L

H

L

Y

Y

Y
|
Y

D
|
D

P
x
Y

H

Q

R

A

L

L

S

P

P

K

E

D

L

A

E

E

R

E

E

K

L

V

N

G

V

A

T

R

F

R

H

V

S

E

S

N

A

I

E

E

S

E

L

L

V

V

R

A

V

Q

C

A

D

D

V

I

I

V

N

T

L

I

Q

N

S
x
A

P
|
P

L

L

Y
x
H

P

A

S

G

T
|
T

E

K

H

G

M

L

F

I

N

N

D

K

A

E

M

L

L

L

A

S

Q

K

I

F

K

K

P

K

G

G

A

A

Y

W

L

L

I

V

N

N

C
x
T

A
|
A

R
|
R

G

G

K

A

L

I

C

C

D

V

A

A

D

E

A

D

V

V

A

A

R

A

A

A

L

L

Q

E

S

S

G

G

R

Q

L

L

A

R

G

G

Y

Y

G

G

D
|
D

V

V

W

W

F

F

P

P

Q

Q

P

P

A

A

P

P

A

K

E

D

H

H

P

P

W

W

R

R

S

D

M

M

P

R

N

N

E

K

-

Y

-

G

-

A

-

G

-

N

G

A

M

M

T

T

P

P

H
|
H

I

Y

S
|
S

G
|
G

A

T

S

T

L

L

S

D

A

A

Q

Q

A

T

R

R

Y

Y

A

A

A

E

G

G

T

T

R

K

E

N

I

I

L

L

E

E

C

S

F

F

L

F

E

T

G

G

R

K

binding azide ion: P68 (= P99), F69 (= F100), V93 (≠ I124), R258 (= R286), H311 (= H334)
binding nicotinamide-adenine-dinucleotide: V93 (≠ I124), N119 (= N148), G173 (= G202), R174 (= R203), I175 (= I204), Y194 (= Y222), D195 (= D223), Y196 (≠ P224), A229 (≠ S257), P230 (= P258), H232 (≠ Y260), T235 (= T263), T256 (≠ C284), A257 (= A285), R258 (= R286), D282 (= D310), H311 (= H334), S313 (= S336), G314 (= G337)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 357

O13437 Formate dehydrogenase; FDH; NAD-dependent formate dehydrogenase; EC 1.17.1.9 from Candida boidinii (Yeast) (see 4 papers)
50% identity, 81% coverage: 50:361/386 of query aligns to 19:338/364 of O13437

query
sites
O13437

E

K

L

L

V

Y

G

G

S
x
C

V

T

S

E

G

N

E

K

L

L

G

G

L

I

R

A

H

N

Y

W

L

L

E

K

S

D

Q

Q

G

G

H

H

E

E

L

L

I

I

V

T

T

T

S

S

D

D

K
|
K

D

E

G

G

P

E

D

T

S

S

I

E

F

L

E

D

R

K

H

H

L

I

C

P

D

D

A

A

D

D

I

I

V

I

I

I

S

T

Q

T

P

P

F
|
F

W

H

P

P

A

A

Y

Y

L

I

T

T

R

K

E

E

R

R

I

L

A

D

K

K

A

A

K

K

K

N

L

L

K

K

L

L

A

V

L

V

T

V

A

A

G

G

I

V

G

G

S

S

D

D

H

H

V

I

D

D

L

L

Q

D

A

Y

A

I

A

N

E

Q

-

T

-

G

R

K

D

K

I

I

T

S

V

V

A

L

E

E

V

V

T

T

F

G

S

S

N
|
N

S

V

I

V

S

S

V

V

A

A

E

E

H

H

V

V

M

M

T

T

V

M

L

L

A

V

L

L

V

V

R

R

N

N

Y

F

L

V

P

P

S

A

H

H

Q

E

Y

Q

A

I

V

I

N

N

G

H

G

D

W

W

N

E

I

V

A

A

D

A

C

I

V

A

A

K

R

D

S

A

Y

Y

D

D

L

I

E

E

G

G

M

K

H

T

F

I

G

A

T

T

I

I

G

G

A

A

G

G

R

R

I
|
I

G

G

L

Y

A

R

V

V

L

L

R

E

R

R

L

L

K

L

P

P

F

F

D

N

-

P

V

K

H

E

L

L

H

L

Y

Y

D

D

P

Y

H
x
Q

R

A

L

L

S

P

P

K

E

E

L

A

E

E

R

E

E

K

L

V

N

G

V

A

T

R

F

R

H

V

S

E

S

N

A

I

E

E

S

E

L

L

V

V

R

A

V

Q

C

A

D

D

V

I

I

V

N

T

L

V

Q

N

S

A

P

P

L

L

Y

H

P

A

S

G

T

T

E

K

H

G

M

L

F

I

N

N

D

K

A

E

M

L

L

L

A

S

Q

K

I

F

K

K

P

K

G

G

A

A

Y

W

L

L

I

V

N

N

C

T

A

A

R
|
R

G

G

K

A

L

I

C
|
C

D

V

A

A

D

E

A

D

V

V

A

A

R

A

A

A

L

L

Q

E

S

S

G

G

R

Q

L

L

A

R

G

G

Y

Y

G

G

D

D

V

V

W

W

F

F

P

P

Q
|
Q

P
|
P

A

A

P

P

A

K

E

D

H

H

P

P

W

W

R

R

S

D

M

M

P

R

N

N

E

K

-

Y

-

G

-

A

-

G

-

N

G

A

M

M

T

T

P

P

H
|
H

I

Y

S

S

G

G

A

T

S

T

L

L

S

D

A

A

Q

Q

A

T

R

R

Y

Y

A

A

A

E

G

G

T

T

R
x
K

E

N

I

I

L

L

E

E

C

S

F

F

L

F

E

T

G

G

R

K

C23 (≠ S54) mutation to S: Slight increase in substrate affinity for formate but no change in affinity for NAD, 9 degrees Celsius decrease in thermal stability compared to the wild-type, significantly higher stability compared to wild-type under biotransformation conditions, significantly more stable in the presence of CuCl(2); when associated with A-262. Large increase in substrate affinity for formate but no significant change in affinity for NAD, 13 degrees Celsius decrease in thermal stability compared to the wild-type, significantly more stable in the presence of CuCl(2); when associated with V-262. No significant change in affinity for formate or NAD, 5 degrees Celsius decrease in thermal stability compared to the wild-type, significantly higher stability compared to wild-type under biotransformation conditions, and significantly more stable in the presence of CuCl(2).
K47 (= K78) mutation to E: Slight increase in substrate affinity for formate and also affinity for NAD increases by half after 2 weeks. Also after 4 months affinity for formate increases by more than half and affinity for NAD increases by more than half. Retains 84% of residual activity after incubation for 20 minutes at a thermal inactivation temperature of 55 degrees Celsius in samples stored for 2 weeks compared to wild-type which loses 50% of its activity at 55 degrees Celsius.
F69 (= F100) mutation to A: 2-fold decrease in substrate affinity for formate, but no significant change in affinity for NAD. A significant reduction in catalytic activity compared to the wild-type.
N119 (= N148) mutation to A: 94-fold decrease in substrate affinity for formate and 2700-fold decrease in substrate affinity for NAD. A significant reduction in catalytic activity compared to the wild-type; when associated with A-311.; mutation to H: 80-fold decrease in substrate affinity for formate and a 1250-fold decrease in substrate affinity for NAD. A significant reduction in catalytic activity compared to the wild-type.
I175 (= I204) mutation to A: 2-fold decrease in substrate affinity for formate and a 12-fold decrease in substrate affinity for NAD. A significant reduction in catalytic activity compared to the wild-type.
Q197 (≠ H225) mutation to L: 4-fold decrease in substrate affinity for formate but no significant change in affinity for NAD compared to the wild-type.
R258 (= R286) mutation to A: No catalytic activity.
C262 (= C290) mutation to A: Slight increase in substrate affinity for formate but no change in affinity for NAD, 9 degrees Celsius decrease in thermal stability compared to the wild-type, greater stability at a higher pH compared to the wild-type; when associated with S-23.; mutation to V: Large increase in substrate affinity for formate but no significant change in affinity for NAD, 13 degrees Celsius decrease in thermal stability compared to the wild-type; when associated with S-23. Great increase in substrate affinity for formate and NAD and 8 degrees Celsius decrease in thermal stability compared to the wild-type.
Q287 (= Q315) mutation to A: 2-fold decrease in substrate affinity for formate and 3-fold decrease in substrate affinity for NAD compared to the wild-type; when associated with A-311.; mutation to E: 380-fold decrease in substrate affinity for formate and 3-fold decrease in substrate affinity for NAD compared to the wild-type; when associated with T-288. No significant decrease in substrate affinity for formate but a 4-fold decrease in substrate affinity for NAD and a significant reduction in catalytic activity compared to the wild-type, a more acidic pH is seen than in the wild-type, preventing formate binding by a single ionization of a group compared to that of the wild-type.
P288 (= P316) mutation to T: 380-fold decrease in substrate affinity for formate and 3-fold decrease in substrate affinity for NAD compared to the wild-type; when associated with E-287.
H311 (= H334) mutation to A: 2-fold decrease in substrate affinity for formate and 3-fold decrease in substrate affinity for NAD compared to the wild-type; when associated with A-287. 93-fold decrease in substrate affinity for formate and 2700-fold decrease in substrate affinity for NAD, and a significant reduction in catalytic activity compared to the wild-type; when associated with A-119.; mutation to Q: 10-fold decrease in substrate affinity for formate and significant reduction in the catalytic activity compared to the wild-type.
K328 (≠ R351) mutation to V: A 75% increase in substrate affinity for formate after 2 weeks and a 50% increase in affinity for NAD. However, after 4 months the affinity for formate increases 7-fold and affinity for NAD increases by 2 thirds. Retains 70% of residual activity after incubation for 20 minutes at a thermal inactivation temperature of 55 degrees Celsius in samples stored for 2 weeks compared to wild-type which loses 50% of its activity at 55 degrees Celsius.

Sites not aligning to the query:

360 K→A: Exhibits no change in substrate affinity for formate, but shows a 4-fold decrease in substrate affinity for NAD implying that L-360 side chain forms strong interactions with the cofactor. A higher reaction rate is observed at an acidic and basic pH values.

Query Sequence

>WP_013093107.1 NCBI__GCF_000092885.1:WP_013093107.1
MAKILCALFPDPVTGYPPSYARTDVPSIASYPGGQRPPSPKGPLGFKPGELVGSVSGELG
LRHYLESQGHELIVTSDKDGPDSIFERHLCDADIVISQPFWPAYLTRERIAKAKKLKLAL
TAGIGSDHVDLQAAAERDITVAEVTFSNSISVAEHVVMTVLALVRNYLPSHQYAVNGGWN
IADCVARSYDLEGMHFGTIGAGRIGLAVLRRLKPFDVHLHYYDPHRLSPELERELNVTFH
SSAESLVRVCDVINLQSPLYPSTEHMFNDAMLAQIKPGAYLINCARGKLCDADAVARALQ
SGRLAGYGGDVWFPQPAPAEHPWRSMPNEGMTPHISGASLSAQARYAAGTREILECFLEG
RAIRAEYLIVNGGKLAGTGAASYKLG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory