SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013134034.1 NCBI__GCF_000092245.1:WP_013134034.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P33221 Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; GAR transformylase T; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 6.3.1.21 from Escherichia coli (strain K12) (see 5 papers)
52% identity, 97% coverage: 3:380/389 of query aligns to 4:382/392 of P33221

query
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P33221

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EL 22:23 (= EL 21:22) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
E82 (= E81) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
R114 (= R113) binding ATP
K155 (= K154) binding ATP
SSGKGQ 160:165 (≠ SSGHGQ 159:164) binding ATP
G162 (= G161) mutation to I: Strong decrease in the reaction rate for the conversion of formate to FGAR and in the affinity for formate. 3- and 2-fold decrease in the affinity for ATP and GAR, respectively.
K179 (≠ E178) modified: N6-acetyllysine
EGVV 195:198 (≠ EEFI 193:196) binding ATP
E203 (= E201) binding ATP
E267 (= E265) binding Mg(2+)
E279 (= E277) binding Mg(2+)
D286 (= D284) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
K355 (= K352) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
RR 362:363 (= RR 359:360) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1kjiA Crystal structure of glycinamide ribonucleotide transformylase in complex with mg-amppcp (see paper)
53% identity, 97% coverage: 3:380/389 of query aligns to 3:379/389 of 1kjiA

query
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1kjiA

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active site: E114 (≠ K114), K154 (= K154), S159 (= S159), G161 (= G161), E264 (= E265), E276 (= E277), D283 (= D284), T284 (= T285), R360 (= R360)
binding phosphomethylphosphonic acid adenylate ester: R113 (= R113), I152 (≠ V152), K154 (= K154), S159 (= S159), S160 (= S160), G161 (= G161), Q164 (= Q164), E192 (= E193), V195 (≠ I196), E200 (= E201), Q222 (= Q223), E264 (= E265), F266 (= F267), E276 (= E277)
binding magnesium ion: E264 (= E265), E276 (= E277)

1ez1A Structure of escherichia coli purt-encoded glycinamide ribonucleotide transformylase complexed with mg, amppnp, and gar (see paper)
53% identity, 97% coverage: 3:380/389 of query aligns to 3:379/389 of 1ez1A

query
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active site: E114 (≠ K114), K154 (= K154), S159 (= S159), G161 (= G161), E264 (= E265), E276 (= E277), D283 (= D284), T284 (= T285), R360 (= R360)
binding phosphoaminophosphonic acid-adenylate ester: R113 (= R113), I152 (≠ V152), K154 (= K154), S159 (= S159), S160 (= S160), G161 (= G161), E192 (= E193), V194 (≠ F195), V195 (≠ I196), F197 (= F198), E200 (= E201), Q222 (= Q223), E264 (= E265), F266 (= F267), E276 (= E277)
binding glycinamide ribonucleotide: G20 (= G20), E21 (= E21), L22 (= L22), E81 (= E81), I82 (≠ V82), S160 (= S160), D283 (= D284), K352 (= K352), R359 (= R359), R360 (= R360)
binding magnesium ion: E264 (= E265), E276 (= E277)

1eyzA Structure of escherichia coli purt-encoded glycinamide ribonucleotide transformylase complexed with mg and amppnp (see paper)
53% identity, 97% coverage: 3:380/389 of query aligns to 3:379/389 of 1eyzA

query
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1eyzA

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S

A

I

T

K

R

I

V

M

M

L

L

G

G

S

S

G

G

E

E

L

L

G

G

K

K

E

E

V

V

I

A

I

I

E

E

A

C

Q

Q

R

R

L

L

G

G

I

V

E

E

T

V

I

I

A

A

V

V

D

D

S

R

Y

Y

N

A

N

D

A

A

P

P

A

A

Q

M

L

H

V

V

A

A

N

H

K

R

A

S

Y

H

T

V

I

I

N

N

M

M

K

L

N

D

K

G

N

D

E

A

I

L

L

R

D

R

V

V

I

V

R

E

R

L

E

E

K

K

P

P

D

H

Y

Y

I

I

L

V

P

P

E

E

V

I

E

E

A

A

I

I

N

A

I

T

D

D

A

M

L

L

F

I

T

Q

A

L

E

E

K

E

E

E

G

G

F

L

H

N

V

V

I

V

P

P

N

C

A

A

E

R

A

A

V

T

N

K

K

L

T

T

M

M

N

N

R
|
R

K
x
E

N

G

I

I

R

R

E

R

F

L

A

A

E

E

L

L

E

Q

L

L

P

P

T

T

S

S

K

T

Y

Y

K

R

F

F

V

A

K

D

S

S

F

E

D

S

E

L

L

F

K

R

N

E

A

A

A

V

E

A

Y

D

I

I

G

G

F

Y

P

P

C

C

V
x
I

I

V

K
|
K

P

P

V

V

M

M

S

S

S
|
S

G
|
G

H

K

G

G

Q
|
Q

S

T

I

F

A

I

K

R

S

S

A

A

K

E

E

Q

L

L

E

A

K

Q

S

A

W

W

E

K

I

Y

A

A

K

Q

E

Q

A

G

R

-

G

G

D

R

A

A

S

G

E

R

L

V

I

I

V

V

E
|
E

E

G

F

V

I
x
V

K

K

F
|
F

D

D

Y

F

E
|
E

I

I

T

T

L

L

T

T

V

V

R

S

N

A

E

V

R

D

Q

G

T

V

I

H

F

F

C

C

A

A

P

P

I

V

G

G

H

H

I

R

Q

Q

K

E

D

D

G

G

D

D

Y

Y

I

R

F

E

S

S

W

W

Q

Q

P

P

M

Q

K

Q

M

M

S

S

D

P

K

L

A

A

I

L

K

E

K

R

S

A

E

Q

E

E

I

I

A

A

K

R

K

K

I

V

T

V

D

L

G

A

L

L

G

G

R

Y

G

G

I

L

F

F

G

G

V

V

E
|
E

L

L

F
|
F

V

V

K

C

G

G

D

D

E

E

V

V

Y

I

F

F

S

S

E
|
E

V

V

S

S

P

P

R

R

P

P

H

H

D
|
D

T
|
T

G

G

M

M

V

V

T

T

M

L

I

I

T

S

Q

Q

S

D

Q

L

S

S

E

E

F

F

A

A

L

L

H

H

V

V

R

R

A

A

V

F

L

L

G

G

L

L

P

P

L

V

N

G

Y

G

I

I

-

R

Q

Q

Y

Y

G

G

A

P

G

A

A

A

S

S

A

A

A

V

Y

I

K

L

A

P

V

Q

A

L

D

T

T

S

F

Q

N

N

P

V

I

T

I

F

D

D

I

N

K

V

D

Q

E

N

A

A

F

V

S

G

D

A

N

D

S

L

Y

Q

V

I

R

R

V

L

F

F

G

G

K

K

P

P

Q

E

S

I

H

D

V

G

G

S

R

R

R
|
R

M

L

A

G

V

V

A

A

L

L

T

A

F

T

D

A

K

E

D

S

S

V

D

D

K

-

A

A

L

I

K

E

N

R

A

A

K

K

active site: E114 (≠ K114), K154 (= K154), S159 (= S159), G161 (= G161), E264 (= E265), E276 (= E277), D283 (= D284), T284 (= T285), R360 (= R360)
binding phosphoaminophosphonic acid-adenylate ester: R113 (= R113), I152 (≠ V152), K154 (= K154), S159 (= S159), S160 (= S160), G161 (= G161), Q164 (= Q164), E192 (= E193), V195 (≠ I196), F197 (= F198), E200 (= E201), E264 (= E265), F266 (= F267), E276 (= E277)
binding magnesium ion: E264 (= E265), E276 (= E277)

1kjjA Crystal structure of glycniamide ribonucleotide transformylase in complex with mg-atp-gamma-s (see paper)
52% identity, 97% coverage: 3:380/389 of query aligns to 3:376/386 of 1kjjA

query
sites
1kjjA

F

L

G

G

T

T

P

A

L

L

K

R

S

P

N

A

S

A

I

T

K

R

I

V

M

M

L

L

G

G

S

S

G

G

E

E

L

L

G

G

K

K

E

E

V

V

I

A

I

I

E

E

A

C

Q

Q

R

R

L

L

G

G

I

V

E

E

T

V

I

I

A

A

V

V

D

D

S

R

Y

Y

N

A

N

D

A

A

P

P

A

A

Q

M

L

H

V

V

A

A

N

H

K

R

A

S

Y

H

T

V

I

I

N

N

M

M

K

L

N

D

K

G

N

D

E

A

I

L

L

R

D

R

V

V

I

V

R

E

R

L

E

E

K

K

P

P

D

H

Y

Y

I

I

L

V

P

P

E

E

V

I

E

E

A

A

I

I

N

A

I

T

D

D

A

M

L

L

F

I

T

Q

A

L

E

E

K

E

E

E

G

G

F

L

H

N

V

V

I

V

P

P

N

C

A

A

E

R

A

A

V

T

N

K

K

L

T

T

M

M

N

N

R
|
R

K
x
E

N

G

I

I

R

R

E

R

F

L

A

A

E

E

L

L

E

Q

L

L

P

P

T

T

S

S

K

T

Y

Y

K

R

F

F

V

A

K

D

S

S

F

E

D

S

E

L

L

F

K

R

N

E

A

A

A

V

E

A

Y

D

I

I

G

G

F

Y

P

P

C

C

V
x
I

I

V

K
|
K

P

P

V

V

M

M

S

S

S
|
S

G
|
G

H

K

G

G

Q
|
Q

S

T

I

F

A

I

K

R

S

S

A

A

K

E

E

Q

L

L

E

A

K

Q

S

A

W

W

E

K

I

Y

A

A

K

Q

E

Q

A

-

R

-

G

-

D

-

A

G

S

G

E

R

L

V

I

I

V

V

E
|
E

E

G

F

V

I
x
V

K

K

F

F

D

D

Y

F

E
|
E

I

I

T

T

L

L

T

T

V

V

R

S

N

A

E

V

R

D

Q

G

T

V

I

H

F

F

C

C

A

A

P

P

I

V

G

G

H

H

I

R

Q
|
Q

K

E

D

D

G

G

D

D

Y

Y

I

R

F

E

S

S

W

W

Q

Q

P

P

M

Q

K

Q

M

M

S

S

D

P

K

L

A

A

I

L

K

E

K

R

S

A

E

Q

E

E

I

I

A

A

K

R

K

K

I

V

T

V

D

L

G

A

L

L

G

G

R

Y

G

G

I

L

F

F

G

G

V

V

E
|
E

L

L

F
|
F

V

V

K

C

G

G

D

D

E

E

V

V

Y

I

F

F

S

S

E
|
E

V

V

S

S

P

P

R

R

P

P

H

H

D
|
D

T
|
T

G

G

M

M

V

V

T

T

M

L

I

I

T

S

Q

Q

S

D

Q

L

S

S

E

E

F

F

A

A

L

L

H

H

V

V

R

R

A

A

V

F

L

L

G

G

L

L

P

P

L

V

N

G

Y

G

I

I

-

R

Q

Q

Y

Y

G

G

A

P

G

A

A

A

S

S

A

A

A

V

Y

I

K

L

A

P

V

Q

A

L

D

T

T

S

F

Q

N

N

P

V

I

T

I

F

D

D

I

N

K

V

D

Q

E

N

A

A

F

V

S

G

D

A

N

D

S

L

Y

Q

V

I

R

R

V

L

F

F

G

G

K

K

P

P

Q

E

S

I

H

D

V

G

G

S

R

R

R
|
R

M

L

A

G

V

V

A

A

L

L

T

A

F

T

D

A

K

E

D

S

S

V

D

D

K

-

A

A

L

I

K

E

N

R

A

A

K

K

active site: E114 (≠ K114), K154 (= K154), S159 (= S159), G161 (= G161), E261 (= E265), E273 (= E277), D280 (= D284), T281 (= T285), R357 (= R360)
binding phosphothiophosphoric acid-adenylate ester: R113 (= R113), I152 (≠ V152), K154 (= K154), S159 (= S159), S160 (= S160), G161 (= G161), Q164 (= Q164), E189 (= E193), V192 (≠ I196), E197 (= E201), Q219 (= Q223), E261 (= E265), F263 (= F267), E273 (= E277)
binding magnesium ion: E261 (= E265), E273 (= E277)

1kj8A Crystal structure of purt-encoded glycinamide ribonucleotide transformylase in complex with mg-atp and gar (see paper)
52% identity, 97% coverage: 3:380/389 of query aligns to 3:376/386 of 1kj8A

query
sites
1kj8A

F

L

G

G

T

T

P

A

L

L

K

R

S

P

N

A

S

A

I

T

K

R

I

V

M

M

L

L

G

G

S

S

G
|
G

E
|
E

L
|
L

G

G

K

K

E

E

V

V

I

A

I

I

E

E

A

C

Q

Q

R

R

L

L

G

G

I

V

E

E

T

V

I

I

A

A

V

V

D

D

S

R

Y

Y

N

A

N

D

A

A

P

P

A

A

Q

M

L

H

V

V

A

A

N

H

K

R

A

S

Y

H

T

V

I

I

N

N

M

M

K

L

N

D

K

G

N

D

E

A

I

L

L

R

D

R

V

V

I

V

R

E

R

L

E

E

K

K

P

P

D

H

Y

Y

I

I

L

V

P

P

E
|
E

V
x
I

E

E

A

A

I

I

N

A

I

T

D

D

A

M

L

L

F

I

T

Q

A

L

E

E

K

E

E

E

G

G

F

L

H

N

V

V

I

V

P

P

N

C

A

A

E

R

A

A

V

T

N

K

K

L

T

T

M

M

N

N

R
|
R

K
x
E

N

G

I

I

R

R

E

R

F

L

A

A

E

E

L

L

E

Q

L

L

P

P

T

T

S

S

K

T

Y

Y

K

R

F

F

V

A

K

D

S

S

F

E

D

S

E

L

L

F

K

R

N

E

A

A

A

V

E

A

Y

D

I

I

G

G

F

Y

P

P

C

C

V
x
I

I

V

K
|
K

P

P

V

V

M

M

S

S

S
|
S

G
|
G

H

K

G

G

Q
|
Q

S

T

I

F

A

I

K

R

S

S

A

A

K

E

E

Q

L

L

E

A

K

Q

S

A

W

W

E

K

I

Y

A

A

K

Q

E

Q

A

-

R

-

G

-

D

-

A

G

S

G

E

R

L

V

I

I

V

V

E
|
E

E

G

F

V

I
x
V

K

K

F
|
F

D

D

Y

F

E
|
E

I

I

T

T

L

L

T

T

V

V

R

S

N

A

E

V

R

D

Q

G

T

V

I

H

F

F

C

C

A

A

P

P

I

V

G

G

H

H

I

R

Q
|
Q

K

E

D

D

G
|
G

D

D

Y

Y

I

R

F

E

S

S

W

W

Q

Q

P

P

M

Q

K

Q

M

M

S

S

D

P

K

L

A

A

I

L

K

E

K

R

S

A

E

Q

E

E

I

I

A

A

K

R

K

K

I

V

T

V

D

L

G

A

L

L

G

G

R

Y

G

G

I

L

F

F

G

G

V

V

E
|
E

L

L

F
|
F

V

V

K

C

G

G

D

D

E

E

V

V

Y

I

F

F

S

S

E
|
E

V

V

S

S

P

P

R

R

P

P

H

H

D
|
D

T
|
T

G

G

M

M

V

V

T

T

M

L

I

I

T

S

Q

Q

S

D

Q

L

S

S

E

E

F

F

A

A

L

L

H

H

V

V

R

R

A

A

V

F

L

L

G

G

L

L

P

P

L

V

N

G

Y

G

I

I

-

R

Q

Q

Y

Y

G

G

A

P

G

A

A

A

S

S

A

A

A

V

Y

I

K

L

A

P

V

Q

A

L

D

T

T

S

F

Q

N

N

P

V

I

T

I

F

D

D

I

N

K

V

D

Q

E

N

A

A

F

V

S

G

D

A

N

D

S

L

Y

Q

V

I

R

R

V

L

F

F

G

G

K
|
K

P

P

Q

E

S

I

H

D

V

G

G

S

R
|
R

M

L

A

G

V

V

A

A

L

L

T

A

F

T

D

A

K

E

D

S

S

V

D

D

K

-

A

A

L

I

K

E

N

R

A

A

K

K

active site: E114 (≠ K114), K154 (= K154), S159 (= S159), G161 (= G161), E261 (= E265), E273 (= E277), D280 (= D284), T281 (= T285), R357 (= R360)
binding adenosine-5'-triphosphate: R113 (= R113), I152 (≠ V152), K154 (= K154), S159 (= S159), S160 (= S160), G161 (= G161), Q164 (= Q164), E189 (= E193), V192 (≠ I196), F194 (= F198), E197 (= E201), Q219 (= Q223), G222 (= G226), E261 (= E265), F263 (= F267), E273 (= E277)
binding glycinamide ribonucleotide: G20 (= G20), E21 (= E21), L22 (= L22), E81 (= E81), I82 (≠ V82), S160 (= S160), D280 (= D284), K349 (= K352), R356 (= R359)
binding magnesium ion: E261 (= E265), E273 (= E277)

1kjqA Crystal structure of glycinamide ribonucleotide transformylase in complex with mg-adp (see paper)
52% identity, 97% coverage: 3:380/389 of query aligns to 3:378/388 of 1kjqA

query
sites
1kjqA

F

L

G

G

T

T

P

A

L

L

K

R

S

P

N

A

S

A

I

T

K

R

I

V

M

M

L

L

G

G

S

S

G

G

E

E

L

L

G

G

K

K

E

E

V

V

I

A

I

I

E

E

A

C

Q

Q

R

R

L

L

G

G

I

V

E

E

T

V

I

I

A

A

V

V

D

D

S

R

Y

Y

N

A

N

D

A

A

P

P

A

A

Q

M

L

H

V

V

A

A

N

H

K

R

A

S

Y

H

T

V

I

I

N

N

M

M

K

L

N

D

K

G

N

D

E

A

I

L

L

R

D

R

V

V

I

V

R

E

R

L

E

E

K

K

P

P

D

H

Y

Y

I

I

L

V

P

P

E

E

V

I

E

E

A

A

I

I

N

A

I

T

D

D

A

M

L

L

F

I

T

Q

A

L

E

E

K

E

E

E

G

G

F

L

H

N

V

V

I

V

P

P

N

C

A

A

E

R

A

A

V

T

N

K

K

L

T

T

M

M

N

N

R
|
R

K
x
E

N

G

I

I

R

R

E

R

F

L

A

A

E

E

L

L

E

Q

L

L

P

P

T

T

S

S

K

T

Y

Y

K

R

F

F

V

A

K

D

S

S

F

E

D

S

E

L

L

F

K

R

N

E

A

A

A

V

E

A

Y

D

I

I

G

G

F

Y

P

P

C

C

V
x
I

I

V

K
|
K

P

P

V

V

M

M

S

S

S

K

S

-

G

-

H

-

G

G

Q

Q

S

T

I

F

A

I

K

R

S

S

A

A

K

E

E

Q

L

L

E

A

K

Q

S

A

W

W

E

K

I

Y

A

A

K

Q

E

Q

A

G

-

G

R

R

G

A

D

G

A

A

S

G

E

R

L

V

I

I

V

V

E
|
E

E

G

F
x
V

I
x
V

K

K

F
|
F

D

D

Y

F

E
|
E

I

I

T

T

L

L

T

T

V

V

R

S

N

A

E

V

R

D

Q

G

T

V

I

H

F

F

C

C

A

A

P

P

I

V

G

G

H

H

I

R

Q
|
Q

K

E

D

D

G

G

D

D

Y

Y

I

R

F

E

S

S

W

W

Q

Q

P

P

M

Q

K

Q

M

M

S

S

D

P

K

L

A

A

I

L

K

E

K

R

S

A

E

Q

E

E

I

I

A

A

K

R

K

K

I

V

T

V

D

L

G

A

L

L

G

G

R

Y

G

G

I

L

F

F

G

G

V

V

E
|
E

L

L

F
|
F

V

V

K

C

G

G

D

D

E

E

V

V

Y

I

F

F

S

S

E
|
E

V

V

S

S

P

P

R

R

P

P

H

H

D
|
D

T
|
T

G

G

M

M

V

V

T

T

M

L

I

I

T

S

Q

Q

S

D

Q

L

S

S

E

E

F

F

A

A

L

L

H

H

V

V

R

R

A

A

V

F

L

L

G

G

L

L

P

P

L

V

N

G

Y

G

I

I

-

R

Q

Q

Y

Y

G

G

A

P

G

A

A

A

S

S

A

A

A

V

Y

I

K

L

A

P

V

Q

A

L

D

T

T

S

F

Q

N

N

P

V

I

T

I

F

D

D

I

N

K

V

D

Q

E

N

A

A

F

V

S

G

D

A

N

D

S

L

Y

Q

V

I

R

R

V

L

F

F

G

G

K

K

P

P

Q

E

S

I

H

D

V

G

G

S

R

R

R
|
R

M

L

A

G

V

V

A

A

L

L

T

A

F

T

D

A

K

E

D

S

S

V

D

D

K

-

A

A

L

I

K

E

N

R

A

A

K

K

active site: E114 (≠ K114), K154 (= K154), E263 (= E265), E275 (= E277), D282 (= D284), T283 (= T285), R359 (= R360)
binding adenosine-5'-diphosphate: R113 (= R113), I152 (≠ V152), K154 (= K154), E191 (= E193), V193 (≠ F195), V194 (≠ I196), F196 (= F198), E199 (= E201), Q221 (= Q223), F265 (= F267), E275 (= E277)
binding magnesium ion: E263 (= E265), E275 (= E277)

O58056 Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 6.3.1.21 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
50% identity, 95% coverage: 2:370/389 of query aligns to 7:393/430 of O58056

query
sites
O58056

K

E

F

L

G

G

T

T

P

A

L

T

K

T

S

D

N

S

S

A

I

Q

K

K

I

I

M

L

L

L

G

G

S

S

G

G

E

E

L

L

G

G

K

K

E

E

V

I

I

A

I

I

E

E

A

A

Q

Q

R

R

L

L

G

G

I

V

E

E

T

V

I

V

A

A

V

V

D

D

S

R

Y

Y

N

A

N

N

A

A

P

P

A

A

Q

M

L

Q

V

V

A

A

N

H

K

R

A

S

Y

Y

T

V

I

G

N

N

M

M

K

M

N

D

K

K

N

D

E

F

I

L

L

W

D

S

V

V

I

V

R

E

R

R

E

E

K

K

P

P

D

D

Y

A

I

I

L

I

P

P

E

E

V

I

E

E

A

A

I

I

N

N

I

L

D

D

A

A

L

L

F

F

T

E

A

F

E

E

K

K

E

D

G

G

F

Y

H

F

V

V

I

V

P

P

N

N

A

A

E

R

A

A

V

T

N

W

K

I

T

A

M

M

N

H

R
|
R

K

E

N

R

I

L

R

R

E

E

F

T

A

L

A

V

E

K

E

E

L

A

E

K

L

V

P

P

T

T

S

S

K

R

Y

Y

K

M

F

Y

V

A

K

T

S

T

F

L

D

D

E

E

L

L

K

Y

N

E

A

A

A

C

E

E

Y

K

I

I

G

G

F

Y

P

P

C

C

V

H

I

T

K
|
K

P

A

V

I

M

M

S

S

G

G

H

K

G

G

Q

S

S

Y

I

F

A

V

K

K

S

G

A

P

K

E

E

D

L

I

E

P

K

K

S

A

W

W

E

E

I

E

A

A

K

K

-

T

E

K

A

A

R

R

G

G

D

S

A

A

S

E

E

K

L

I

I

I

V

V

E
|
E

F
x
H

I
|
I

K

D

F

F

D

D

Y

V

E
|
E

I

V

T

T

L

E

L

L

T

A

V

V

R

R

N

H

-

F

-

D

-

E

-

N

-

G

E

E

R

I

Q

V

T

T

I

T

F

F

C

P

A

K

P

P

I

V

G

G

H

H

I

Y

Q

Q

K

I

D

D

G

G

D

D

Y

Y

I

H

F

A

S

S

W

W

Q

Q

P

P

M

A

K

E

M

I

S

S

D

E

K

K

A

A

I

E

K

R

K

E

S

V

E

Y

E

R

I

I

A

A

K

K

K

R

I

I

T

T

D

D

G

V

L

L

G

G

R

L

G

G

I

I

F

F

G

G

V

V

E

E

L

M

F

F

V

V

K

K

G

G

D

D

E

K

V

V

Y

W

F

A

S

N

E

E

V

V

S

S

P

P

R

R

P

P

H

H

D

D

T

T

G

G

M

M

V

V

T

T

M

L

I

A

T

S

Q

H

S

P

Q

P

-

G

-

F

S

S

E

E

F

F

A

A

L

L

H

H

V

L

R

R

A

A

V

V

L

L

G

G

L

L

P

P

L

I

N

P

-

G

-

E

-

W

-

V

-

D

-

G

-

Y

-

R

-

L

Y

F

I

P

Q

M

Y

L

G

I

A

P

G

A

A

A

S

T

A

H

A

V

Y

I

K

K

A

A

V

K

A

V

D

S

T

G

F

Y

N

S

P

P

I

R

I

F

D

R

I

G

K

L

D

V

E

K

A

A

F

L

S

S

-

V

D

P

N

N

S

A

Y

T

V

V

R

R

V

L

F

F

G

G

K

K

P

P

Q

E

S

A

H

Y

V

V

G

G

R

R

M

L

A

G

V

I

A

A

L

L

T

A

F

W

D

D

K

K

D

D

R118 (= R113) binding ATP
K159 (= K154) binding ATP
EEHI 199:202 (≠ EEFI 193:196) binding ATP
E207 (= E201) binding ATP

2dwcB Crystal structure of probable phosphoribosylglycinamide formyl transferase from pyrococcus horikoshii ot3 complexed with adp
48% identity, 95% coverage: 2:370/389 of query aligns to 9:382/409 of 2dwcB

query
sites
2dwcB

K

E

F

L

G

G

T

T

P

A

L

T

K

T

S

D

N

S

S

A

I

Q

K

K

I

I

M

L

L

L

G

G

S

S

G

G

E

E

L

L

G

G

K

K

E

E

V

I

I

A

I

I

E

E

A

A

Q

Q

R

R

L

L

G

G

I

V

E

E

T

V

I

V

A

A

V

V

D

D

S

R

Y

Y

N

A

N

N

A

A

P

P

A

A

Q

M

L

Q

V

V

A

A

N

H

K

R

A

S

Y

Y

T

V

I

G

N

N

M

M

K

M

N

D

K

K

N

D

E

F

I

L

L

W

D

S

V

V

I

V

R

E

R

R

E

E

K

K

P

P

D

D

Y

A

I

I

L

I

P

P

E

E

V

I

E

E

A

A

I

I

N

N

I

L

D

D

A

A

L

L

F

F

T

E

A

F

E

E

K

K

E

D

G

G

F

Y

H

F

V

V

I

V

P

P

N

N

A

A

E

R

A

A

V

T

N

W

K

I

T

A

M

M

N

H

R
|
R

K

E

N

R

I

L

R

R

E

E

F

T

A

L

A

V

E

K

E

E

L

A

E

K

L

V

P

P

T

T

S

S

K

R

Y

Y

K

M

F

Y

V

A

K

T

S

T

F

L

D

D

E

E

L

L

K

Y

N

E

A

A

A

C

E

E

Y

K

I

I

G

G

F

Y

P

P

C

C

V
x
H

I

T

K
|
K

P

A

V

I

M

M

S

S

S

-

G

-

H

-

G

G

Q

S

S

Y

I

F

A

V

K

K

S

G

A

P

K

E

E

D

L

I

E

P

K

K

S

A

W

W

E

E

I

E

A

E

K

K

E

-

A

-

R

-

G

-

D

-

A

-

S

-

E

-

L

I

I

I

V

V

E

E

F
x
H

I
|
I

K

D

F
|
F

D

D

Y

V

E
|
E

I

V

T

T

L

E

L

L

T

A

V

V

R

R

N

H

-

F

-

D

-

E

-

N

-

G

E

E

R

I

Q

V

T

T

I

T

F

F

C

P

A

K

P

P

I

V

G

G

H

H

I

Y

Q

Q

K

I

D

D

G

G

D

D

Y

Y

I

H

F

A

S

S

W

W

Q

Q

P

P

M

A

K

E

M

I

S

S

D

E

K

K

A

A

I

E

K

R

K

E

S

V

E

Y

E

R

I

I

A

A

K

K

K

R

I

I

T

T

D

D

G

V

L

L

G

G

R

L

G

G

I

I

F

F

G

G

V

V

E
|
E

L

M

F
|
F

V

V

K

K

G

G

D

D

E

K

V

V

Y

W

F

A

S

N

E
|
E

V

V

S

S

P

P

R

R

P

P

H

H

D
|
D

T
|
T

G

G

M

M

V

V

T

T

M

L

I

A

T

S

Q

H

S

P

Q

P

-

G

-

F

S

S

E

E

F

F

A

A

L

L

H

H

V

L

R

R

A

A

V

V

L

L

G

G

L

L

P

P

L

I

N

P

-

G

-

E

-

W

-

V

-

D

-

G

-

Y

-

R

-

L

Y

F

I

P

Q

M

Y

L

G

I

A

P

G

A

A

A

S

T

A

H

A

V

Y

I

K

K

A

A

V

K

A

V

D

S

T

G

F

Y

N

S

P

P

I

R

I

F

D

R

I

G

K

L

D

V

E

K

A

A

F

L

S

S

-

V

D

P

N

N

S

A

Y

T

V

V

R

R

V

L

F

F

G

G

K

K

P

P

Q

E

S

A

H

Y

V

V

G

G

R

R

R
|
R

M

L

A

G

V

I

A

A

L

L

T

A

F

W

D

D

K

K

D

D

active site: E265 (= E265), E277 (= E277), D284 (= D284), T285 (= T285), R372 (= R360)
binding adenosine-5'-diphosphate: R120 (= R113), H159 (≠ V152), K161 (= K154), H190 (≠ F195), I191 (= I196), F193 (= F198), E196 (= E201), F267 (= F267), E277 (= E277)

3ax6A Crystal structure of n5-carboxyaminoimidazole ribonucleotide synthetase from thermotoga maritima
27% identity, 93% coverage: 28:388/389 of query aligns to 18:360/360 of 3ax6A

query
sites
3ax6A

I

L

E

E

A

A

Q

K

R

K

L

M

G

G

I

F

E

Y

T

V

I

I

A

V

V

L

D

D

S

P

Y

T

N

P

N

R

A

S

P

P

A

A

Q

G

L

Q

V

V

A

A

N

D

K

E

A

Q

Y

I

T

V

I

A

N

G

M

F

K

F

N

D

K

S

N

E

E

R

I

I

L

E

D

D

V

L

I

V

R

K

R

-

E

-

K

G

P

S

D

D

Y

V

I

T

L

T

P

Y

E

D

V

L

E

E

A

H

I

I

N

D

I

V

D

Q

A

T

L

L

F

K

T

K

A

L

E

Y

K

N

E

E

G

G

F

Y

H

K

V

I

I

H

P

P

N

S

A

P

E

Y

A

T

V

L

N

E

K

I

T

I

M

Q

N

D

R
x
K

K

F

N

V

I

Q

R

K

E

E

F

F

A

L

A

K

E

K

E

N

L

-

E

G

L

I

P

P

T

V

S

P

K

E

Y

Y

K

K

F

L

V

V

K

K

S

-

F

-

D

-

E

D

L

L

K

E

N

S

A

D

A

V

E

R

Y

E

I

F

G

G

F

F

P

P

C

V

V
|
V

I

Q

K
|
K

P

-

V

-

M

-

S

-

S

A

S

R

G

K

H

G

G

G

Q

V

S

F

I

I

A

I

K

K

S

N

A

E

K

K

E

D

L

L

E

E

K

N

S

A

W

I

E

K

I

-

A

-

K

-

E

-

A

-

R

-

G

-

D

-

A

-

S

G

E

E

L

T

I

Y

V

L

E
|
E

E

E

F
|
F

I
x
V

K

E

F

I

D

E

Y

K

E
|
E

I

L

T

A

L

V

L

M

T

V

V

A

R

R

N

N

E

E

R

K

Q

G

T

E

I

I

F

A

C

C

A

Y

P

P

I

V

G

V

H

E

I

M

Q

-

K

-

D

-

G

-

D

-

Y

Y

I

D

F

T

S

V

W

I

Q

A

P

P

M

A

K

R

M

I

S

E

D

E

K

K

A

Y

I

S

K

K

K

I

S

A

E

R

E

E

I

I

A

A

K

T

K

S

I

V

T

V

D

E

G

A

L

L

G

E

G

G

R

V

G

G

I

I

F

F

G

G

V

I

E
|
E

L

M

F
|
F

V

L

-

T

K

K

G

Q

D

G

E

E

V

I

Y

L

F

V

S
x
N

E
|
E

V

I

S

A

P

P

R

R

P

P

H

H

D
x
N

T
x
S

G

G

M

H

V

Y

T

T

M

I

I

E

T

A

Q

C

S

V

Q

T

S

S

E

Q

F

F

A

E

L

Q

H

H

V

I

R

R

A

A

V

I

L

M

G

N

L

L

P

P

L

L

N

G

Y

S

I

T

Q

E

Y

L

G

L

A

I

G

P

A

A

S

V

A

M

A

V

Y

N

K

L

A

L

V

G

A

E

D

E

T

G

F

Y

-

Y

-

G

N

K

P

P

I

A

I

L

D

I

I

G

K

L

D

E

E

E

A

A

F

L

S

A

-

I

D

E

N

G

S

L

Y

S

V

L

R

H

V

F

F

Y

G

G

K

K

P

K

Q

E

S

T

H

R

V

P

G

Y

R

R

R
x
K

M

M

A

G

V

H

A

F

L

T

T

V

F

V

D

D

K

R

D

D

-

V

-

E

-

R

S

A

S

L

D

E

K

K

A

A

L

L

K

R

N

-

A

A

K

K

E

K

I

I

I

L

K

K

N

V

F

V

S

S

D

E

active site: E231 (= E265), E244 (= E277), N251 (≠ D284), S252 (≠ T285), K330 (≠ R360)
binding adenosine-5'-diphosphate: K101 (≠ R113), V136 (= V152), K138 (= K154), E164 (= E193), F166 (= F195), V167 (≠ I196), E172 (= E201), F233 (= F267), N243 (≠ S276)

4dlkA Crystal structure of atp-ca++ complex of purk: n5- carboxyaminoimidazole ribonucleotide synthetase (see paper)
25% identity, 93% coverage: 21:382/389 of query aligns to 18:369/380 of 4dlkA

query
sites
4dlkA

E

Q

L

L

G

G

K

R

E

M

V

M

I

A

I

L

E

A

A

A

Q

K

R

E

L

M

G

G

I

Y

E

K

T

I

I

A

A

V

V

L

D

D

S

P

Y

T

N

K

N

N

A

S

P

P

A

C

Q

A

L
x
Q

V

V

A
|
A

N

D

K

-

A

-

Y

-

T

-

I

I

N

E

M

I

K

V

N

A

K

S

N

Y

E

D

I

D

L

L

D

K

V

A

I

I

R

Q

R

H

-

L

-

A

E

E

K

I

P

S

D

D

Y

V

I

V

L

T

P

Y

E
|
E

V
x
F

E

E

A

N

I

I

N

D

I

Y

D

R

A

C

L

L

F

Q

T

W

A

L

E

E

K

K

E

H

G

A

F

Y

H

-

V

-

I

L

P

P

N

Q

-

G

A

S

E

Q

A

L

V

L

N

S

K

K

T

T

M

Q

N

N

R
|
R

-

F

-

T

-

E

K

K

N

N

I

-

R

-

E

-

F

-

A

A

A

I

E

E

E

K

L

A

E

G

L

L

P

P

T

V

S

A

K

T

Y

Y

K

R

F

L

V

V

K

Q

S

N

F

Q

D

E

E

Q

L

L

K

T

N

E

A

A

A

I

E

A

Y

E

I

L

G

S

F

Y

P

P

C

S

V

V

I

L

K
|
K

P

T

V

T

M

T

S

G

-
x
Y

S
x
D

G
|
G

H

K

G

G

Q
|
Q

S

V

I

V

A

L

K

R

S

S

A

E

K

A

E

D

L

V

E

D

K

E

S

A

W

R

E

K

I

L

A

A

K

-

E

-

A

-

R

-

G

-

D

N

A

A

S

A

E

E

L

C

I

I

V

L

E

E

E

K

F
x
W

I
x
V

K

P

F
|
F

D

E

Y

K

E
|
E

I

V

T

S

L

V

L

I

T

V

V

I

R

R

N

S

E

V

R

S

Q

G

T

E

I

T

F

K

C

V

A

F

P

P

I

V

G

A

-

E

H

N

I

I

Q

H

K

V

D

N

G

N

D

I

Y

L

I

H

F

E

S

S

W

I

Q

V

P

P

M

A

K

R

M

I

S

T

D

E

K

E

A

L

I

S

K

Q

K

K

S

A

E

I

E

A

I

Y

A

A

K

K

K

V

I

L

T

A

D

D

G

E

L

L

G

E

G

L

R

V

G

G

I

T

F

L

G

A

V

V

E
|
E

L

M

F
|
F

V

A

K

T

G

A

D

D

-

G

E

E

V

I

Y

Y

F

I

S
x
N

E
|
E

V

L

S

A

P

P

R
|
R

P

P

H
|
H

D
x
N

T
x
S

G

G

M

H

V

Y

T

T

M

Q

I

D

T

A

Q

C

S

E

Q

T

S

S

E

Q

F

F

A

G

L

Q

H

H

V

I

R

R

A

A

V

I

L

C

G

N

L

L

P

P

L

L

N

-

Y

-

I

-

Q

-

Y

-

G

-

A

-

G

G

A

E

S

T

A

N

A

L

Y

L

K

K

A

P

V

V

A

V

D

-

T

-

F

-

N

-

P

-

I

M

I

V

D

N

I

I

K

L

D

G

E

E

A

H

F

I

S

E

-

G

-

V

-

L

-

R

-

Q

-

V

-

N

-

R

-

L

D

T

N

G

S

C

Y

Y

V

L

R

H

V

L

F

Y

G

G

K
|
K

P

E

Q

E

S

A

H

K

V

A

G

Q

R
|
R

R
x
K

M

M

A

G

V

-

A

H

L

V

T

N

F

I

D

L

K

N

D

D

S

N

S

I

D

E

K

V

A

A

L

L

K

E

N

K

A

A

K

K

E

S

I

L

active site: Y153 (vs. gap), G155 (= G161), E255 (= E265), E268 (= E277), N275 (≠ D284), S276 (≠ T285), K348 (≠ R360)
binding adenosine-5'-triphosphate: E76 (= E81), F77 (≠ V82), R107 (= R113), K147 (= K154), Y153 (vs. gap), D154 (≠ S160), G155 (= G161), Q158 (= Q164), W184 (≠ F195), V185 (≠ I196), F187 (= F198), E190 (= E201), E255 (= E265), F257 (= F267), N267 (≠ S276), E268 (= E277), R272 (= R281), H274 (= H283), N275 (≠ D284), K340 (= K352), R347 (= R359), K348 (≠ R360)
binding calcium ion: E255 (= E265), E268 (= E277)
binding phosphate ion: Q47 (≠ L50), A49 (= A52)

3q2oB Crystal structure of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
25% identity, 93% coverage: 21:382/389 of query aligns to 18:369/377 of 3q2oB

query
sites
3q2oB

E

Q

L

L

G

G

K

R

E

M

V

M

I

A

I

L

E

A

A

A

Q

K

R

E

L

M

G

G

I

Y

E

K

T

I

I

A

A

V

V

L

D

D

S

P

Y

T

N

K

N

N

A

S

P

P

A

C

Q

A

L

Q

V

V

A

A

N

D

K

-

A

-

Y

-

T

-

I

I

N

E

M

I

K

V

N

A

K

S

N

Y

E

D

I

D

L

L

D

K

V

A

I

I

R

Q

R

H

-

L

-

A

E

E

K

I

P

S

D

D

Y

V

I

V

L

T

P

Y

E

E

V

F

E

E

A

N

I

I

N

D

I

Y

D

R

A

C

L

L

F

Q

T

W

A

L

E

E

K

K

E

H

G

A

F

Y

H

-

V

-

I

L

P

P

N

Q

-

G

A

S

E

Q

A

L

V

L

N

S

K

K

T
|
T

M

Q

N

N

R

R

-

F

-

T

-
x
E

K

K

N

N

I

-

R

-

E

-

F

-

A

A

A

I

E

E

E

K

L

A

E

G

L

L

P

P

T

V

S

A

K

T

Y

Y

K

R

F

L

V

V

K

Q

S

N

F

Q

D

E

E

Q

L

L

K

T

N

E

A

A

A

I

E

A

Y

E

I

L

G

S

F

Y

P

P

C

S

V

V

I

L

K

K

P

T

V

T

M

T

S

G

-

Y

S

D

G

G

H

K

G

G

Q

Q

S

V

I

V

A

L

K

R

S

S

A

E

K

A

E

D

L

V

E

D

K

E

S

A

W

R

E

K

I

L

A

A

K

-

E

-

A

-

R

-

G

-

D

N

A

A

S

A

E

E

L

C

I

I

V

L

E

E

E

K

F

W

I

V

K

P

F

F

D

E

Y

K

E

E

I

V

T

S

L

V

L

I

T

V

V

I

R

R

N

S

E

V

R

S

Q

G

T

E

I

T

F

K

C

V

A

F

P

P

I

V

G

A

-

E

H

N

I

I

Q

H

K

V

D

N

G

N

D

I

Y

L

I

H

F

E

S

S

W

I

Q

V

P

P

M

A

K

R

M

I

S

T

D

E

K

E

A

L

I

S

K

Q

K

K

S

A

E

I

E

A

I

Y

A

A

K

K

K

V

I

L

T

A

D

D

G

E

L

L

G

E

G

L

R

V

G

G

I

T

F

L

G

A

V

V

E

E

L

M

F

F

V

A

K

T

G

A

D

D

-

G

E

E

V

I

Y

Y

F

I

S

N

E

E

V
x
L

S

A

P

P

R

R

P

P

H

H

D

N

T

S

G

G

M

H

V

Y

T

T

M

Q

I

D

T

A

Q

C

S

E

Q

T

S

S

E

Q

F

F

A

G

L

Q

H

H

V

I

R

R

A

A

V

I

L

C

G

N

L

L

P

P

L

L

N

-

Y

-

I

-

Q

-

Y

-

G

-

A

-

G

G

A

E

S

T

A

N

A

L

Y

L

K

K

A

P

V

V

A

V

D

-

T

-

F

-

N

-

P

-

I

M

I

V

D

N

I

I

K

L

D

G

E

E

A

H

F

I

S

E

-

G

-

V

-

L

-

R

-

Q

-

V

-

N

-

R

-

L

D

T

N

G

S

C

Y

Y

V

L

R

H

V

L

F

Y

G

G

K

K

P

E

Q

E

S

A

H

K

V

A

G

Q

R

R

R

K

M

M

A

G

V

-

A

H

L

V

T

N

F

I

D

L

K

N

D

D

S

N

S

I

D

E

K

V

A

A

L

L

K

E

N

K

A

A

K

K

E

S

I

L

binding magnesium ion: T104 (= T110), E110 (vs. gap), L269 (≠ V278)

3v4sA Crystal structure of adp-atp complex of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
25% identity, 93% coverage: 21:382/389 of query aligns to 17:368/380 of 3v4sA

query
sites
3v4sA

E

Q

L

L

G

G

K

R

E

M

V

M

I

A

I

L

E

A

A

A

Q

K

R

E

L

M

G

G

I

Y

E

K

T

I

I

A

A

V

V

L

D

D

S

P

Y

T

N

K

N

N

A

S

P

P

A

C

Q

A

L

Q

V

V

A

A

N

D

K

-

A

-

Y

-

T

-

I

I

N

E

M

I

K

V

N

A

K

S

N

Y

E

D

I

D

L

L

D

K

V

A

I

I

R

Q

R

H

-

L

-

A

E

E

K

I

P

S

D

D

Y

V

I

V

L

T

P

Y

E

E

V

F

E

E

A

N

I

I

N

D

I

Y

D

R

A

C

L

L

F

Q

T

W

A

L

E

E

K

K

E

H

G

A

F

Y

H

-

V

-

I

L

P

P

N

Q

-

G

A

S

E

Q

A

L

V

L

N

S

K

K

T

T

M

Q

N

N

R
|
R

-

F

-

T

-

E

K

K

N

N

I

-

R

-

E

-

F

-

A

A

A

I

E

E

E

K

L

A

E

G

L

L

P

P

T

V

S

A

K

T

Y

Y

K

R

F

L

V

V

K

Q

S

N

F

Q

D

E

E

Q

L

L

K

T

N

E

A

A

A

I

E

A

Y

E

I

L

G

S

F

Y

P

P

C

S

V

V

I

L

K
|
K

P

T

V

T

M

T

S

G

-
x
Y

S

D

G
|
G

H

K

G

G

Q
|
Q

S

V

I

V

A

L

K

R

S

S

A

E

K

A

E

D

L

V

E

D

K

E

S

A

W

R

E

K

I

L

A

A

K

-

E

-

A

-

R

-

G

-

D

N

A

A

S

A

E

E

L

C

I

I

V

L

E

E

E

K

F
x
W

I
x
V

K

P

F

F

D

E

Y

K

E
|
E

I

V

T

S

L

V

L

I

T

V

V

I

R

R

N

S

E

V

R

S

Q

G

T

E

I

T

F

K

C

V

A

F

P

P

I

V

G

A

-

E

H

N

I

I

Q

H

K

V

D

N

G
x
N

D

I

Y

L

I

H

F

E

S

S

W

I

Q

V

P

P

M

A

K

R

M

I

S

T

D

E

K

E

A

L

I

S

K

Q

K

K

S

A

E

I

E

A

I

Y

A

A

K

K

K

V

I

L

T

A

D

D

G

E

L

L

G

E

G

L

R

V

G

G

I

T

F

L

G

A

V

V

E

E

L

M

F
|
F

V

A

K

T

G

A

D

D

-

G

E

E

V

I

Y

Y

F

I

S
x
N

E
|
E

V

L

S

A

P

P

R
|
R

P

P

H
|
H

D
x
N

T

S

G

G

M

H

V

Y

T

T

M

Q

I

D

T

A

Q

C

S

E

Q

T

S

S

E

Q

F

F

A

G

L

Q

H

H

V

I

R

R

A

A

V

I

L

C

G

N

L

L

P

P

L

L

N

-

Y

-

I

-

Q

-

Y

-

G

-

A

-

G

G

A

E

S

T

A

N

A

L

Y

L

K

K

A

P

V

V

A

V

D

-

T

-

F

-

N

-

P

-

I

M

I

V

D

N

I

I

K

L

D

G

E

E

A

H

F

I

S

E

-

G

-

V

-

L

-

R

-

Q

-

V

-

N

-

R

-

L

D

T

N

G

S

C

Y

Y

V

L

R

H

V

L

F

Y

G

G

K

K

P

E

Q

E

S

A

H

K

V

A

G

Q

R

R

R

K

M

M

A

G

V

-

A

H

L

V

T

N

F

I

D

L

K

N

D

D

S

N

S

I

D

E

K

V

A

A

L

L

K

E

N

K

A

A

K

K

E

S

I

L

binding adenosine-5'-diphosphate: R106 (= R113), K146 (= K154), Y152 (vs. gap), G154 (= G161), Q157 (= Q164), W183 (≠ F195), V184 (≠ I196), E189 (= E201), N215 (≠ G226), F256 (= F267), N266 (≠ S276), E267 (= E277)
binding carbonate ion: R271 (= R281), H273 (= H283), N274 (≠ D284)

3v4sB Crystal structure of adp-atp complex of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
25% identity, 93% coverage: 21:382/389 of query aligns to 19:370/381 of 3v4sB

query
sites
3v4sB

E

Q

L

L

G

G

K

R

E

M

V

M

I

A

I

L

E

A

A

A

Q

K

R

E

L

M

G

G

I

Y

E

K

T

I

I

A

A

V

V

L

D

D

S

P

Y

T

N

K

N

N

A

S

P

P

A

C

Q

A

L

Q

V

V

A

A

N

D

K

-

A

-

Y

-

T

-

I

I

N

E

M

I

K

V

N

A

K

S

N

Y

E

D

I

D

L

L

D

K

V

A

I

I

R

Q

R

H

-

L

-

A

E

E

K

I

P

S

D

D

Y

V

I

V

L

T

P

Y

E

E

V

F

E

E

A

N

I

I

N

D

I

Y

D

R

A

C

L

L

F

Q

T

W

A

L

E

E

K

K

E

H

G

A

F

Y

H

-

V

-

I

L

P

P

N

Q

-

G

A

S

E

Q

A

L

V

L

N

S

K

K

T
|
T

M

Q

N

N

R
|
R

-

F

-

T

-
x
E

K

K

N

N

I

-

R

-

E

-

F

-

A

A

A

I

E

E

E

K

L

A

E

G

L

L

P

P

T

V

S

A

K

T

Y

Y

K

R

F

L

V

V

K

Q

S

N

F

Q

D

E

E

Q

L

L

K

T

N

E

A

A

A

I

E

A

Y

E

I

L

G

S

F

Y

P

P

C

S

V

V

I

L

K
|
K

P

T

V

T

M

T

S

G

-
x
Y

S
x
D

G
|
G

H

K

G

G

Q
|
Q

S

V

I

V

A

L

K

R

S

S

A

E

K

A

E

D

L

V

E

D

K

E

S

A

W

R

E

K

I

L

A

A

K

-

E

-

A

-

R

-

G

-

D

N

A

A

S

A

E

E

L

C

I

I

V

L

E
|
E

E

K

F
x
W

I
x
V

K

P

F
|
F

D

E

Y

K

E
|
E

I

V

T

S

L

V

L

I

T

V

V

I

R

R

N

S

E

V

R

S

Q

G

T

E

I

T

F

K

C

V

A

F

P

P

I

V

G

A

-

E

H

N

I

I

Q
x
H

K

V

D

N

G
x
N

D

I

Y

L

I

H

F

E

S

S

W

I

Q

V

P

P

M

A

K

R

M

I

S

T

D

E

K

E

A

L

I

S

K

Q

K

K

S

A

E

I

E

A

I

Y

A

A

K

K

K

V

I

L

T

A

D

D

G

E

L

L

G

E

G

L

R

V

G

G

I

T

F

L

G

A

V

V

E
|
E

L

M

F
|
F

V

A

K

T

G

A

D

D

-

G

E

E

V

I

Y

Y

F

I

S

N

E
|
E

V
x
L

S

A

P

P

R
|
R

P

P

H
|
H

D
x
N

T

S

G

G

M

H

V

Y

T

T

M

Q

I

D

T

A

Q

C

S

E

Q

T

S

S

E

Q

F

F

A

G

L

Q

H

H

V

I

R

R

A

A

V

I

L

C

G

N

L

L

P

P

L

L

N

-

Y

-

I

-

Q

-

Y

-

G

-

A

-

G

G

A

E

S

T

A

N

A

L

Y

L

K

K

A

P

V

V

A

V

D

-

T

-

F

-

N

-

P

-

I

M

I

V

D

N

I

I

K

L

D

G

E

E

A

H

F

I

S

E

-

G

-

V

-

L

-

R

-

Q

-

V

-

N

-

R

-

L

D

T

N

G

S

C

Y

Y

V

L

R

H

V

L

F

Y

G

G

K

K

P

E

Q

E

S

A

H

K

V

A

G

Q

R

R

R

K

M

M

A

G

V

-

A

H

L

V

T

N

F

I

D

L

K

N

D

D

S

N

S

I

D

E

K

V

A

A

L

L

K

E

N

K

A

A

K

K

E

S

I

L

binding adenosine-5'-triphosphate: R108 (= R113), K148 (= K154), Y154 (vs. gap), D155 (≠ S160), G156 (= G161), Q159 (= Q164), E183 (= E193), W185 (≠ F195), V186 (≠ I196), F188 (= F198), E191 (= E201), H214 (≠ Q223), N217 (≠ G226), E256 (= E265), F258 (= F267), E269 (= E277)
binding carbonate ion: R273 (= R281), H275 (= H283), N276 (≠ D284)
binding magnesium ion: T105 (= T110), E111 (vs. gap), E256 (= E265), E269 (= E277), L270 (≠ V278)

3r5hA Crystal structure of adp-air complex of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
25% identity, 93% coverage: 21:382/389 of query aligns to 18:369/383 of 3r5hA

query
sites
3r5hA

E
x
Q

L
|
L

G

G

K

R

E

M

V

M

I

A

I

L

E

A

A

A

Q

K

R

E

L

M

G

G

I

Y

E

K

T

I

I

A

A

V

V

L

D

D

S

P

Y

T

N

K

N

N

A

S

P

P

A

C

Q

A

L

Q

V

V

A

A

N

D

K

-

A

-

Y

-

T

-

I

I

N

E

M

I

K

V

N

A

K

S

N

Y

E

D

I

D

L

L

D

K

V

A

I

I

R

Q

R

H

-

L

-

A

E

E

K

I

P

S

D

D

Y

V

I

V

L

T

P

Y

E
|
E

V

F

E

E

A

N

I

I

N

D

I

Y

D

R

A

C

L

L

F

Q

T

W

A

L

E

E

K

K

E

H

G

A

F

Y

H

-

V

-

I

L

P

P

N

Q

-

G

A

S

E

Q

A

L

V

L

N

S

K

K

T

T

M

Q

N

N

R
|
R

-

F

-

T

-

E

K

K

N

N

I

-

R

-

E

-

F

-

A

A

A

I

E

E

E

K

L

A

E

G

L

L

P

P

T

V

S

A

K

T

Y

Y

K

R

F

L

V

V

K

Q

S

N

F

Q

D

E

E

Q

L

L

K

T

N

E

A

A

A

I

E

A

Y

E

I

L

G

S

F

Y

P

P

C

S

V

V

I

L

K
|
K

P

T

V

T

M

T

S

G

-
x
Y

S

D

G

G

H

K

G

G

Q
|
Q

S

V

I

V

A

L

K

R

S

S

A

E

K

A

E

D

L

V

E

D

K

E

S

A

W

R

E

K

I

L

A

A

K

-

E

-

A

-

R

-

G

-

D

N

A

A

S

A

E

E

L

C

I

I

V

L

E

E

E

K

F
x
W

I
x
V

K

P

F
|
F

D

E

Y

K

E
|
E

I

V

T

S

L

V

L

I

T

V

V

I

R

R

N

S

E

V

R

S

Q

G

T

E

I

T

F

K

C

V

A

F

P

P

I

V

G

A

-

E

H

N

I

I

Q

H

K

V

D

N

G
x
N

D

I

Y

L

I

H

F

E

S

S

W

I

Q

V

P

P

M

A

K

R

M

I

S

T

D

E

K

E

A

L

I

S

K

Q

K

K

S

A

E

I

E

A

I

Y

A

A

K

K

K

V

I

L

T

A

D

D

G

E

L

L

G

E

G

L

R

V

G

G

I

T

F

L

G

A

V

V

E

E

L

M

F
|
F

V

A

K

T

G

A

D

D

-

G

E

E

V

I

Y

Y

F

I

S
x
N

E
|
E

V

L

S

A

P

P

R
|
R

P

P

H

H

D

N

T

S

G

G

M

H

V

Y

T

T

M

Q

I

D

T

A

Q

C

S

E

Q

T

S

S

E

Q

F

F

A

G

L

Q

H

H

V

I

R

R

A

A

V

I

L

C

G

N

L

L

P

P

L

L

N

-

Y

-

I

-

Q

-

Y

-

G

-

A

-

G

G

A

E

S

T

A

N

A

L

Y

L

K

K

A

P

V

V

A

V

D

-

T

-

F

-

N

-

P

-

I

M

I

V

D

N

I

I

K

L

D

G

E

E

A

H

F

I

S

E

-

G

-

V

-

L

-

R

-

Q

-

V

-

N

-

R

-

L

D

T

N

G

S

C

Y

Y

V

L

R

H

V

L

F

Y

G

G

K
|
K

P

E

Q

E

S

A

H

K

V

A

G

Q

R
|
R

R

K

M

M

A

G

V

-

A

H

L

V

T

N

F

I

D

L

K

N

D

D

S

N

S

I

D

E

K

V

A

A

L

L

K

E

N

K

A

A

K

K

E

S

I

L

binding adenosine-5'-diphosphate: R107 (= R113), K147 (= K154), Q158 (= Q164), W184 (≠ F195), V185 (≠ I196), F187 (= F198), E190 (= E201), N216 (≠ G226), F257 (= F267), N267 (≠ S276), E268 (= E277)
binding 5-aminoimidazole ribonucleotide: Q18 (≠ E21), L19 (= L22), E76 (= E81), Y153 (vs. gap), R272 (= R281), K340 (= K352), R347 (= R359)

Sites not aligning to the query:

binding 5-aminoimidazole ribonucleotide: 17

4ma0A The crystal structure of phosphoribosylaminoimidazole carboxylase atpase subunit of francisella tularensis subsp. Tularensis schu s4 in complex with partially hydrolysed atp
28% identity, 77% coverage: 12:310/389 of query aligns to 1:292/366 of 4ma0A

query
sites
4ma0A

I

M

K

K

I

I

M

G

L

I

G

G

S

A

G

G

E

Q

L

L

G

A

K

R

E

M

V

L

I

S

I

L

E

A

A

G

Q

T

R

P

L

L

G

G

I

L

E

E

T

F

I

H

A

C

V

L

D

G

S

K

Y

-

N

N

N

G

A

D

P

C

A

A

Q

E

L

E

V

V

A

V

N

K

K

T

A

V

Y

T

T

D

I

I

N

E

M

L

K

T

N

K

K

V

N

N

E

D

I

V

L

-

D

-

V

V

I

A

R

W

R

A

E

K

K

Q

P

F

D

D

Y

V

I

I

L

T

P

F

E

E

V

N

E

E

A

N

I

I

N

S

I

H

D

E

A

-

L

L

F

I

T

K

A

A

E

I

K

N

E

H

G

E

F

V

H

S

V

V

I

Y

P

P

N

S

A

A

E

K

A

A

V

I

N

A

K

I

T

S

M

Q

N

D

R

R

K

L

N

L

I

E

R

K

E

S

F

F

A

M

A

-

E

Q

E

D

L

H

E

G

L

I

P

A

T

T

S

A

K

K

Y

F

K

V

F

N

V

I

K

D

S

S

F

L

D

A

E

K

L

L

K

Q

N

S

A

A

A

V

E

D

Y

D

I

H

G

G

F

L

P

P

C

A

V
x
I

I

L

K
|
K

P

T

V

R

-

R

M

F

S

G

S
x
Y

S

D

G
|
G

H

K

G

G

Q

Q

S

F

I

V

A

I

K

R

S

S

A

Q

K

E

E

D

L

I

E

T

K

K

S

A

W

W

E

D

I

V

A

L

K

K

E

D

A

A

R

-

G

-

D

-

A

P

S

D

E

G

L

L

I

I

V

Y

E
|
E

E
x
A

F
|
F

I
x
V

K

D

F

F

D

D

Y

Y

E
|
E

I

V

T

S

L

Q

L

I

T

C

V

T

R

A

N

D

E

L

R

K

Q

G

T

N

I

I

F

A

C

F

A

Y

P

P

I

L

G

A

-

R

H

N

I

T

Q
x
H

K

K

D

Q

G

G

D

I

Y

I

I

V

F

E

S

S

W

E

Q

A

P

P

M

F

K

E

M

N

S

V

D

V

K

L

A

A

I

-

K

E

K

K

S

A

E

Q

I

I

A

A

K

K

K

I

I

L

T

V

D

K

G

E

L

F

G

A

G

Y

R

V

G

G

I

T

F

L

G

A

V

I

E
|
E

L

F

F
|
F

V

V

K

K

G

G

D

D

E

E

V

L

Y

I

F

V

S

N

E
|
E

V

I

S

A

P

P

R

R

P

V

H

H

D
x
N

T
x
S

G

G

M

H

V

W

T

S

M

I

I

D

T

G

Q

A

S

V

Q

T

S

S

E

Q

F

F

A

E

L

N

H

H

V

V

R

R

A

A

V

I

L

A

G

G

L

L

P

I

L

L

active site: Y144 (≠ S159), G146 (= G161), E247 (= E265), E259 (= E277), N266 (≠ D284), S267 (≠ T285)
binding adenosine monophosphate: I136 (≠ V152), K138 (= K154), E175 (= E193), A176 (≠ E194), F177 (= F195), V178 (≠ I196), E183 (= E201), H206 (≠ Q223), F249 (= F267), E259 (= E277)

Sites not aligning to the query:

active site: 335

5jqwA The crystal structure of phosphoribosylaminoimidazole carboxylase atpase subunit of francisella tularensis subsp. Tularensis schu s4 in complex with adp
28% identity, 77% coverage: 12:310/389 of query aligns to 1:292/365 of 5jqwA

query
sites
5jqwA

I

M

K

K

I

I

M

G

L

I

G

G

S

A

G

G

E

Q

L

L

G

A

K

R

E

M

V

L

I

S

I

L

E

A

A

G

Q

T

R

P

L

L

G

G

I

L

E

E

T

F

I

H

A

C

V

L

D

G

S

K

Y

-

N

N

N

G

A

D

P

C

A

A

Q

E

L

E

V

V

A

V

N

K

K

T

A

V

Y

T

T

D

I

I

N

E

M

L

K

T

N

K

K

V

N

N

E

D

I

V

L

-

D

-

V

V

I

A

R

W

R

A

E

K

K

Q

P

F

D

D

Y

V

I

I

L

T

P

F

E

E

V

N

E

E

A

N

I

I

N

S

I

H

D

E

A

-

L

L

F

I

T

K

A

A

E

I

K

N

E

H

G

E

F

V

H

S

V

V

I

Y

P

P

N

S

A

A

E

K

A

A

V

I

N

A

K

I

T

S

M

Q

N

D

R
|
R

K

L

N

L

I

E

R

K

E

S

F

F

A

M

A

Q

E

D

E

H

L

-

E

G

L

I

P

A

T

T

S

A

K

K

Y

F

K

V

F

N

V

I

K

D

S

S

F

L

D

A

E

K

L

L

K

Q

N

S

A

A

A

V

E

D

Y

D

I

H

G

G

F

L

P

P

C

A

V

I

I

L

K
|
K

P

T

V

R

-

R

M

F

S
x
G

S
x
Y

S
x
D

G
|
G

H

K

G

G

Q

Q

S

F

I

V

A

I

K

R

S

S

A

Q

K

E

E

D

L

I

E

T

K

K

S

A

W

W

E

D

I

V

A

L

K

K

E

D

A

A

R

-

G

-

D

-

A

P

S

D

E

G

L

L

I

I

V

Y

E

E

E

A

F

F

I
x
V

K

D

F

F

D

D

Y

Y

E
|
E

I

V

T

S

L

Q

L

I

T

C

V

T

R

A

N

D

E

L

R

K

Q

G

T

N

I

I

F

A

C

F

A

Y

P

P

I

L

G

A

-

R

H

N

I

T

Q
x
H

K

K

D

Q

G

G

D

I

Y

I

I

V

F

E

S

S

W

E

Q

A

P

P

M

F

K

E

M

N

S

V

D

V

K

L

A

A

I

-

K

E

K

K

S

A

E

Q

I

I

A

A

K

K

K

I

I

L

T

V

D

K

G

E

L

F

G

A

G

Y

R

V

G

G

I

T

F

L

G

A

V

I

E
|
E

L

F

F
|
F

V

V

K

K

G

G

D

D

E

E

V

L

Y

I

F

V

S

N

E
|
E

V

I

S

A

P

P

R

R

P

V

H

H

D
x
N

T
x
S

G

G

M

H

V

W

T

S

M

I

I

D

T

G

Q

A

S

V

Q

T

S

S

E

Q

F

F

A

E

L

N

H

H

V

V

R

R

A

A

V

I

L

A

G

G

L

L

P

I

L

L

active site: Y144 (≠ S159), G146 (= G161), E247 (= E265), E259 (= E277), N266 (≠ D284), S267 (≠ T285)
binding adenosine-5'-diphosphate: R98 (= R113), K138 (= K154), G143 (≠ S158), Y144 (≠ S159), D145 (≠ S160), G146 (= G161), V178 (≠ I196), E183 (= E201), H206 (≠ Q223), F249 (= F267), E259 (= E277)

Sites not aligning to the query:

active site: 335

4mamA The crystal structure of phosphoribosylaminoimidazole carboxylase atpase subunit of francisella tularensis subsp. Tularensis schu s4 in complex with an adp analog, amp-cp
28% identity, 77% coverage: 12:310/389 of query aligns to 1:292/373 of 4mamA

query
sites
4mamA

I

M

K

K

I

I

M

G

L

I

G

G

S

A

G

G

E

Q

L

L

G

A

K

R

E

M

V

L

I

S

I

L

E

A

A

G

Q

T

R

P

L

L

G

G

I

L

E

E

T

F

I

H

A

C

V

L

D

G

S

K

Y

-

N

N

N

G

A

D

P

C

A

A

Q

E

L

E

V

V

A

V

N

K

K

T

A

V

Y

T

T

D

I

I

N

E

M

L

K

T

N

K

K

V

N

N

E

D

I

V

L

-

D

-

V

V

I

A

R

W

R

A

E

K

K

Q

P

F

D

D

Y

V

I

I

L

T

P

F

E

E

V

N

E

E

A

N

I

I

N

S

I

H

D

E

A

-

L

L

F

I

T

K

A

A

E

I

K

N

E

H

G

E

F

V

H

S

V

V

I

Y

P

P

N

S

A

A

E

K

A

A

V

I

N

A

K

I

T

S

M

Q

N

D

R
|
R

K

L

N

L

I

E

R

K

E

S

F

F

A

M

A

-

E

Q

E

D

L

H

E

G

L

I

P

A

T

T

S

A

K

K

Y

F

K

V

F

N

V

I

K

D

S

S

F

L

D

A

E

K

L

L

K

Q

N

S

A

A

A

V

E

D

Y

D

I

H

G

G

F

L

P

P

C

A

V
x
I

I

L

K
|
K

P

T

V

R

-

R

M

F

S

G

S
x
Y

S

D

G
|
G

H

K

G

G

Q
|
Q

S

F

I

V

A

I

K

R

S

S

A

Q

K

E

E

D

L

I

E

T

K

K

S

A

W

W

E

D

I

V

A

L

K

K

E

D

A

A

R

-

G

-

D

-

A

P

S

D

E

G

L

L

I

I

V

Y

E
|
E

E

A

F
|
F

I
x
V

K

D

F
|
F

D

D

Y

Y

E
|
E

I

V

T

S

L

Q

L

I

T

C

V

T

R

A

N

D

E

L

R

K

Q

G

T

N

I

I

F

A

C

F

A

Y

P

P

I

L

G

A

-

R

H

N

I

T

Q
x
H

K

K

D

Q

G

G

D

I

Y

I

I

V

F

E

S

S

W

E

Q

A

P

P

M

F

K

E

M

N

S

V

D

V

K

L

A

A

I

-

K

E

K

K

S

A

E

Q

I

I

A

A

K

K

K

I

I

L

T

V

D

K

G

E

L

F

G

A

G

Y

R

V

G

G

I

T

F

L

G

A

V

I

E
|
E

L

F

F
|
F

V

V

K

K

G

G

D

D

E

E

V

L

Y

I

F

V

S

N

E
|
E

V

I

S

A

P

P

R

R

P

V

H

H

D
x
N

T
x
S

G

G

M

H

V

W

T

S

M

I

I

D

T

G

Q

A

S

V

Q

T

S

S

E

Q

F

F

A

E

L

N

H

H

V

V

R

R

A

A

V

I

L

A

G

G

L

L

P

I

L

L

active site: Y144 (≠ S159), G146 (= G161), E247 (= E265), E259 (= E277), N266 (≠ D284), S267 (≠ T285)
binding phosphomethylphosphonic acid adenosyl ester: R98 (= R113), I136 (≠ V152), K138 (= K154), Y144 (≠ S159), G146 (= G161), Q149 (= Q164), E175 (= E193), F177 (= F195), V178 (≠ I196), F180 (= F198), E183 (= E201), H206 (≠ Q223), F249 (= F267), E259 (= E277)

Sites not aligning to the query:

active site: 335
binding phosphomethylphosphonic acid adenosyl ester: 371

4ma5A The crystal structure of phosphoribosylaminoimidazole carboxylase atpase subunit of francisella tularensis subsp. Tularensis schu s4 in complex with an atp analog, amp-pnp.
27% identity, 77% coverage: 12:310/389 of query aligns to 1:290/363 of 4ma5A

query
sites
4ma5A

I

M

K

K

I

I

M

G

L

I

G

G

S

A

G

G

E

Q

L

L

G

A

K

R

E

M

V

L

I

S

I

L

E

A

A

G

Q

T

R

P

L

L

G

G

I

L

E

E

T

F

I

H

A

C

V

L

D

G

S

K

Y

-

N

N

N

G

A

D

P

C

A

A

Q

E

L

E

V

V

A

V

N

K

K

T

A

V

Y

T

T

D

I

I

N

E

M

L

K

T

N

K

K

V

N

N

E

D

I

V

L

-

D

-

V

V

I

A

R

W

R

A

E

K

K

Q

P

F

D

D

Y

V

I

I

L

T

P

F

E

E

V

N

E

E

A

N

I

I

N

S

I

H

D

E

A

-

L

L

F

I

T

K

A

A

E

I

K

N

E

H

G

E

F

V

H

S

V

V

I

Y

P

P

N

S

A

A

E

K

A

A

V

I

N

A

K

I

T

S

M

Q

N

D

R

R

K

L

N

L

I

E

R

K

E

S

F

F

A

M

A

Q

E

D

E

H

L

-

E

G

L

I

P

A

T

T

S

A

K

K

Y

F

K

V

F

N

V

I

K

D

S

S

F

L

D

A

E

K

L

L

K

Q

N

S

A

A

A

V

E

D

Y

D

I

H

G

G

F

L

P

P

C

A

V
x
I

I

L

K
|
K

P

T

V

R

-

R

M

F

S

G

S
x
Y

S
x
D

G
|
G

H

K

G

G

Q

Q

S

F

I

V

A

I

K

R

S

S

A

Q

K

E

E

D

L

I

E

T

K

K

S

A

W

W

E

D

I

V

A

L

K

K

E

D

A

A

R

-

G

-

D

-

A

P

S

D

E

G

L

L

I

I

V

Y

E

E

E

A

F
|
F

I
x
V

K

D

F

F

D

D

Y

Y

E
|
E

I

V

T

S

L

Q

L

I

T

C

V

T

R

A

N

D

E

L

R

K

Q

G

T

N

I

I

F

A

C

F

A

Y

P

P

I

L

G

A

-

R

H

N

I

T

Q
x
H

K

K

D

Q

G

G

D

I

Y

I

I

V

F

E

S

S

W

E

Q

A

P

P

M

F

K

E

M

N

S

V

D

V

K

L

A

A

I

-

K

E

K

K

S

A

E

Q

I

I

A

A

K

K

K

I

I

L

T

V

D

K

G

E

L

F

G

A

G

Y

R

V

G

G

I

T

F

L

G

A

V

I

E
|
E

L

F

F
|
F

V

V

K

K

G

-

D

-

E

E

V

L

Y

I

F

V

S
x
N

E
|
E

V

I

S

A

P

P

R

R

P

V

H
|
H

D
x
N

T
x
S

G

G

M

H

V

W

T

S

M

I

I

D

T

G

Q

A

S

V

Q

T

S

S

E

Q

F

F

A

E

L

N

H

H

V

V

R

R

A

A

V

I

L

A

G

G

L

L

P

I

L

L

active site: Y144 (≠ S159), G146 (= G161), E247 (= E265), E257 (= E277), N264 (≠ D284), S265 (≠ T285)
binding phosphoaminophosphonic acid-adenylate ester: I136 (≠ V152), K138 (= K154), D145 (≠ S160), G146 (= G161), F177 (= F195), V178 (≠ I196), E183 (= E201), H206 (≠ Q223), E247 (= E265), F249 (= F267), N256 (≠ S276), E257 (= E277), H263 (= H283)

Sites not aligning to the query:

active site: 333

3k5iA Crystal structure of n5-carboxyaminoimidazole synthase from aspergillus clavatus in complex with adp and 5-aminoimadazole ribonucleotide (see paper)
24% identity, 72% coverage: 30:310/389 of query aligns to 23:300/381 of 3k5iA

query
sites
3k5iA

A

A

Q

N

R

R

L

L

G

N

I

I

E

Q

T

V

I

N

A

V

V

L

D

D

S

A

Y

D

N

N

N

S

A

P

P

A

A

K

Q

Q

L

I

V

S

A

A

N

H

K

D

A

G

Y

H

T

V

I

T

N

G

M

S

K

F

N

K

K

E

N

R

E

E

I

A

L

V

D

R

V

Q

I

L

R

A

R

K

E

-

K

T

P

C

D

D

Y

V

I

V

L

T

P

A

E
|
E

V
x
I

E

E

A

H

I

V

N

D

I

T

D

Y

A

A

L

L

F

E

T

E

A

V

E

A

K

S

E

E

G

-

F

V

H

K

V

I

I

E

P

P

N

S

A

W

E

Q

A

A

V

I

N

R

K

T

T

I

M

Q

N

N

R
x
K

K

F

N

N

I

Q

R

K

E

E

F

H

A

L

A

-

E

R

E

K

L

Y

E

G

L

I

P

P

T

M

S

A

K

E

Y

H

K

R

F

E

V

L

-

V

-

E

K

N

S

T

F

P

D

A

E

E

L

L

K

A

N

K

A

V

A

G

E

E

Y

Q

I

L

G

G

F

Y

P

P

C

L

V

M

I

L

K
|
K

P

S

-

K

V

T

M

M

S

A

S
x
Y

S
x
D

G
|
G

H
x
R

G

G

Q

N

S

F

I

R

A

V

K

N

S

S

A

Q

K

D

E

D

L

I

E

P

K

E

S

A

W

L

E

E

I

A

A

L

K

K

E

D

A

-

R

-

G

-

D

-

A

-

S

R

E

P

L

L

I

Y

V

A

E

E

E

K

F
x
W

I
x
A

K

Y

F
|
F

D

K

Y

M

E
|
E

I

L

T

A

L

V

L

I

T

V

V

V

R

K

N

T

E

K

R

D

Q

E

T

V

I

L

F

S

C

Y

A

P

P

T

I

V

G

E

H

T

I

V

Q
|
Q

K

E

D

D

G
x
S

D

I

Y

C

I

K

F

L

S

V

W

Y

Q

A

P

P

M

A

K

R

-

N

M

V

S

S

D

D

K

A

A

I

I

N

K

Q

K

K

S

A

E

Q

E

E

I

L

A

A

K

R

K

K

I

A

T

V

D

A

G

A

L

F

G

D

G

G

R

K

G

G

I

V

F

F

G

G

V

V

E
|
E

L

M

F

F

-

L

V

L

K

E

G

D

D

D

E

S

V

I

Y

M

F

L

S

C

E
|
E

V

I

S

A

P

S

R
|
R

P

I

H

H

D
x
N

T
x
S

G

G

M

H

V

Y

T

T

M

I

I

E

T

G

Q

C

S

A

Q

L

S

S

E

Q

F

F

A

D

L

A

H

H

V

L

R

R

A

A

V

I

L

L

G

D

L

L

P

P

L

I

active site: E254 (= E265), E267 (= E277), N274 (≠ D284), S275 (≠ T285)
binding adenosine-5'-diphosphate: K104 (≠ R113), K146 (= K154), Y152 (≠ S159), D153 (≠ S160), G154 (= G161), W183 (≠ F195), A184 (≠ I196), F186 (= F198), E189 (= E201), Q211 (= Q223), S214 (≠ G226), E267 (= E277)
binding 5-aminoimidazole ribonucleotide: E73 (= E81), I74 (≠ V82), Y152 (≠ S159), D153 (≠ S160), R155 (≠ H162), R271 (= R281)
binding magnesium ion: E254 (= E265), E267 (= E277)

Sites not aligning to the query:

active site: 353
binding 5-aminoimidazole ribonucleotide: 13, 14, 15, 345, 352

Query Sequence

>WP_013134034.1 NCBI__GCF_000092245.1:WP_013134034.1
MKFGTPLKSNSIKIMLLGSGELGKEVIIEAQRLGIETIAVDSYNNAPAQLVANKAYTINM
KNKNEILDVIRREKPDYILPEVEAINIDALFTAEKEGFHVIPNAEAVNKTMNRKNIREFA
AEELELPTSKYKFVKSFDELKNAAEYIGFPCVIKPVMSSSGHGQSIAKSAKELEKSWEIA
KEARGDASELIVEEFIKFDYEITLLTVRNERQTIFCAPIGHIQKDGDYIFSWQPMKMSDK
AIKKSEEIAKKITDGLGGRGIFGVELFVKGDEVYFSEVSPRPHDTGMVTMITQSQSEFAL
HVRAVLGLPLNYIQYGAGASAAYKAVADTFNPIIDIKDEAFSDNSYVRVFGKPQSHVGRR
MAVALTFDKDSSDKALKNAKEIIKNFSDC

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory