SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013134073.1 NCBI__GCF_000092245.1:WP_013134073.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q09179 Glutamine synthetase; GS; Glutamate--ammonia ligase; EC 6.3.1.2 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
48% identity, 98% coverage: 3:330/335 of query aligns to 27:356/359 of Q09179

query
sites
Q09179

K

K

T

V

K

M

L

A

E

E

Y

Y

I

I

W

W

L

I

D

D

G

G

Y

F

K

-

P

-

T

-

Q

N

S

H

L

L

R

R

S

S

K

K

T

T

M

M

V

T

V

L

S

D

D

A

F

K

S

P

G

S

K

S

L

I

E

D

D

Q

C

L

D

R

I

V

W

W

S

N

F

F

D

D

G

G

S

S

T

T

K

G

Q

Q

A

A

V

P

G

G

N

N

S

S

D

D

C

T

L

L

K

K

P

P

V

V

A

A

L

M

Y

Y

L

N

D

D

P

P

-

F

G

R

R

R

I

G

D

D

A

N

Y

I

I

L

V

V

I

L

N

A

E

A

V

C

L

Y

N

T

S

A

D

D

E

G

T

S

P

P

H

N

I

G

S

F

N

N

K

H

R

R

-

D

-

A

-

C

-

A

A

K

T

L

I

L

E

E

E

K

N

H

D

A

D

D

-

K

-

E

F

T

W

W

F

F

G

G

F

I

E

E

Q

Q

E

E

Y

Y

T

T

L

M

I

L

D

D

L

Y

E

-

T

Y

N

D

R

R

P

P

I

F

G

G

F

W

P

P

V

K

N

G

G

G

Y

F

P

P

S

G

P

P

Q

Q

G

G

Q

P

Y

F

Y

Y

C

C

S

G

V

V

G

G

A

T

T

G

N

R

A

V

V

F

G

A

R

R

Q

D

I

I

I

V

E

E

E

A

H

H

L

Y

D

K

T

A

C

C

I

L

E

Y

A

A

G

G

L

I

N

N

I

I

E

S

G

G

I

I

N

N

S

A

E

E

V

V

M

M

M

P

G

S

Q

Q

W

W

E

E

Y

Y

Q

Q

I

V

F

G

S

P

K

C

N

A

A

G

T

I

E

E

A

M

C

G

D

D

Q

Q

I

L

W

W

A

M

S

S

R

R

Y

F

F

L

L

L

E

H

R

R

I

I

T

A

E

E

Q

D

Y

F

N

G

V

V

K

K

V

I

D

S

W

F

D

H

P

P

K

K

P

P

V

I

Q

L

G

G

D

D

W

W

N

N

G

G

S

A

G

G

M

C

H

H

A

T

N

N

F

V

S

S

N

T

K

K

Q

D

L

T

R

R

E

A

S

E

G

G

D

G

K

I

K

K

I

A

F

I

D

E

K
x
S

I

Y

C

L

E

E

E

K

F

F

K

A

L

K

T

R

H

H

K

K

E

E

H

H

I

I

A

A

V

V

Y

Y

G

G

D

D

H

D

N

N

E

D

Q

L

R

R

L

L

T

T

G

G

E

R

H

H

E

E

T
|
T

Q

G

D
x
S

I

I

N

D

S

K

F

F

S

T

Y

Y

G

G

V

V

S

A

D

D

R

R

S

G

A

A

S

S

L

V

R

R

I

I

P

P

I

-

A

R

T

S

I

V

K

A

N

M

N

N

W

G

K

C

G

G

R

Y

I

F

E

E

D

D

R

R

P

P

A

A

S

S

N

S

A

I

N

D

P

P

Y

Y

D

L

V

V

A

T

A

G

I

I

I

T

K

E

S

T

V

M

S273 (≠ K246) modified: Phosphoserine
T303 (= T276) modified: Phosphothreonine
S305 (≠ D278) modified: Phosphoserine

Q9LVI8 Glutamine synthetase cytosolic isozyme 1-3; GS1; Glutamate--ammonia ligase GLN1;3; GLN1;3; EC 6.3.1.2 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
46% identity, 98% coverage: 3:330/335 of query aligns to 18:351/354 of Q9LVI8

query
sites
Q9LVI8

K

K

T

I

K

I

L

A

E

E

Y

Y

I

I

W

W

L

I

D

G

G

G

Y

S

K

G

P

-

T

-

Q

M

S

D

L

I

R

R

S

S

K

K

T

A

M

R

V

T

V

L

S

P

D

G

F

P

S

V

G

T

K

D

L

P

E

S

D
x
K

C

L

D

P

I

K

W

W

S

N

F

Y

D

D

G

G

S

S

T

T

K

G

Q

Q

A

A

V

A

G

G

N

E

N

D

S

S

D

E

C

V

L

I

L

L

K

Y

P

P

V

Q

A

A

L

I

Y

F

L

K

D

D

P

P

G

F

R

R

I

K

-

G

D

N

A

N

Y

I

I

L

V

V

I

M

N

C

E

D

V

A

L

Y

N

T

S

P

D

A

E

G

T

D

P

P

H

I

I

P

S

T

N

N

K

K

R

R

-

H

-

N

-

A

A

A

T

K

I

I

E

F

E

S

N

H

D

P

D

D

F

V

-

A

-

K

-

E

-

P

W

W

F

Y

G

G

F

I

E

E

Q

Q

E

E

Y

Y

T

T

L

L

I

M

D

Q

L

K

E

D

T

V

N

N

R

W

P

P

I

I

G

G

F

W

P

P

V

V

N

G

G

G

Y

Y

P

P

S

G

P

P

Q

Q

G

G

Q

P

Y

Y

C

C

S

G

V

V

G

G

A

A

T

D

N

K

A

A

V

I

G

G

R

R

Q

D

I

I

I

V

E

D

E
x
A

H

H

L

Y

D

K

T

A

C

C

I

L

E

Y

A

A

G

G

L

I

N

G

I

I

E

S

G

G

I

I

N

N

S

G

E

E

V

V

M

M

M

P

G

G

Q

Q

W

W

E

E

Y

F

Q

Q

I

V

F

G

S

P

K

V

N

E

A

G

T

I

E

S

A

S

C

G

D

D

Q

Q

I

V

W

W

A

V

S

A

R

R

Y

Y

F

L

L

L

E

E

R

R

I

I

T

T

E

E

Q

I

Y

S

N

G

V

V

K

I

V

V

D

S

W

F

D

D

P

P

K

K

P

P

V

V

Q

P

G

G

D

D

W

W

N

N

G

G

S

A

G

G

M

A

H

H

A

C

N

N

F

Y

S

S

N

T

K

K

Q

T

L

M

R

R

E

N

S

D

G

G

D

G

K

L

K

E

I

V

F

I

D

K

K

K

I

A

C

I

E

G

E

K

F

L

K

Q

L

L

T

K

H

H

K

K

E

E

H

H

I

I

A

A

V

A

Y

Y

G

G

D

E

H

G

N

N

E

E

Q

R

R

R

L

L

T

T

G

G

E

K

H

H

E

E

T

T

Q

A

D

D

I

I

N

N

S

T

F

F

S

S

Y

W

G

G

V

V

S

A

D

N

R

R

S

G

A

A

S

S

L

V

R

R

I

V

P

G

I

R

A

D

T

T

I

E

K

K

N

E

N

G

W

-

K

K

G

G

R

Y

I

F

E

E

D

D

R

R

P

P

A

A

S

S

N

N

A

M

N

D

P

P

Y

Y

D

V

V

V

A

T

A

S

I

M

I

I

I

A

K

E

S

T

V

T

K49 (≠ D36) mutation to Q: 3-fold increase in affinity for ammonium and catalytic efficiency.
A174 (≠ E152) mutation to S: 4-fold increase in affinity for ammonium and catalytic efficiency.

2d3cA Crystal structure of the maize glutamine synthetase complexed with adp and phosphinothricin phosphate (see paper)
44% identity, 98% coverage: 3:330/335 of query aligns to 16:349/353 of 2d3cA

query
sites
2d3cA

K

K

T

I

K

I

L

A

E

E

Y

Y

I

I

W

W

L

I

D

G

G

G

Y

S

K

G

P

-

T

-

Q

M

S

D

L

L

R

R

S

S

K

K

T

A

M

R

V

T

V

L

S

P

D

G

F

P

S

V

G

T

K

D

L

P

E

S

D

K

C

L

D

P

I

K

W

W

S

N

F

Y

D

D

G

G

S

S

T

T

K

G

Q

Q

A

A

V

P

G

G

N

E

N

D

S

S

D

E

C

V

L

I

L

L

K

Y

P

P

V

Q

A

A

L

I

Y

F

L

K

D

D

P

P

-

F

G

R

R

R

I

G

D

N

A

N

Y

I

I

L

V

V

I

M

N

C

E

D

V

C

L

Y

N

T

S

P

D

A

E

G

T

E

P

P

H

I

I

P

S

T

N

N

K

K

R

R

A

Y

T

S

I

A

E

A

E

K

-

I

-

F

-

S

-

P

-

E

-

V

-

A

N

A

D

E

F

P

W

W

F

Y

G

G

F

I

E
|
E

Q

Q

E
|
E

Y

Y

T

T

L

L

I

L

D

Q

L

K

E

D

T

T

N

N

R

W

P

P

I

L

G

G

F

W

P

P

V

I

N

G

G

G

Y

F

P

P

S

G

P

P

Q

Q

G

G

Q

P

Y

Y

C

C

S

G

V

I

G

G

A

A

T

E

N

K

A

S

V

F

G

G

R

R

Q

D

I

I

I

V

E

D

E

A

H

H

L

Y

D

K

T

A

C

C

I

L

E

Y

A

A

G

G

L

I

N

N

I

I

E
x
S

G

G

I

I

N

N

S

G

E
|
E

V

V

M

M

M

P

G

G

Q

Q

W

W

E
|
E

Y

F

Q
|
Q

I

V

F

G

S

P

K

S

N

V

A

G

T

I

E

S

A

S

C

G

D

D

Q

Q

I

V

W

W

A

V

S

A

R

R

Y

Y

F

I

L

L

E

E

R

R

I

I

T

T

E

E

Q

I

Y

A

N

G

V

V

K

V

V

V

D

T

W

F

D

D

P

P

K

K

P

P

V

I

Q

P

G

G

D

D

W

W

N
|
N

G
|
G

S

A

G

G

M

A

H
|
H

A

T

N
|
N

F
x
Y

S
|
S

N

T

K

E

Q

S

L

M

R

R

E

K

S

E

G

G

D

G

K

Y

K

E

I

V

F

I

D

K

K

A

I

A

C

I

E

E

E

K

F

L

K

K

L

L

T

R

H

H

K

K

E

E

H

H

I

I

A

A

V

A

Y

Y

G

G

D

E

H

G

N

N

E

E

Q

R

R
|
R

L

L

T

T

G

G

E

R

H

H

E
|
E

T

T

Q

A

D

D

I

I

N

N

S

T

F

F

S

S

Y

W

G

G

V

V

S

A

D

N

R
|
R

S

G

A

A

S

S

L

V

R
|
R

I

V

P

G

I

R

A

E

T

T

I

E

K

Q

N

N

N

G

W

-

K

K

G

G

R
x
Y

I

F

E
|
E

D

D

R
|
R

R

R

P

P

A

A

S

S

N

N

A

M

N

D

P

P

Y

Y

D

V

V

V

A

T

A

S

I

M

I

I

I

A

K

E

S

T

V

T

binding adenosine-5'-diphosphate: E127 (= E107), S185 (≠ E165), Q199 (= Q179), N249 (= N229), Y250 (≠ F230), S251 (= S231), R314 (= R294), Y326 (≠ R307), E328 (= E309)
binding manganese (ii) ion: E127 (= E107), E127 (= E107), E129 (= E109), E190 (= E170), E197 (= E177), E197 (= E177), H247 (= H227), E328 (= E309)
binding (2s)-2-amino-4-[methyl(phosphonooxy)phosphoryl]butanoic acid: E129 (= E109), E190 (= E170), E197 (= E177), N242 (= N222), G243 (= G223), H247 (= H227), R289 (= R269), E295 (= E275), R309 (= R289), R330 (= R311)

2d3bA Crystal structure of the maize glutamine synthetase complexed with amppnp and methionine sulfoximine (see paper)
44% identity, 98% coverage: 3:330/335 of query aligns to 16:349/353 of 2d3bA

query
sites
2d3bA

K

K

T

I

K

I

L

A

E

E

Y

Y

I

I

W

W

L

I

D

G

G

G

Y

S

K

G

P

-

T

-

Q

M

S

D

L

L

R

R

S

S

K

K

T

A

M

R

V

T

V

L

S

P

D

G

F

P

S

V

G

T

K

D

L

P

E

S

D

K

C

L

D

P

I

K

W

W

S

N

F

Y

D

D

G

G

S

S

T

T

K

G

Q

Q

A

A

V

P

G

G

N

E

N

D

S

S

D

E

C

V

L

I

L

L

K

Y

P

P

V

Q

A

A

L

I

Y

F

L

K

D

D

P

P

-

F

G

R

R

R

I

G

D

N

A

N

Y

I

I

L

V

V

I

M

N

C

E

D

V

C

L

Y

N

T

S

P

D

A

E

G

T

E

P

P

H

I

I

P

S

T

N

N

K

K

R

R

A

Y

T

S

I

A

E

A

E

K

-

I

-

F

-

S

-

P

-

E

-

V

-

A

N

A

D

E

F

P

W

W

F

Y

G
|
G

F

I

E
|
E

Q

Q

E
|
E

Y

Y

T

T

L

L

I

L

D

Q

L

K

E

D

T

T

N

N

R

W

P

P

I

L

G

G

F

W

P

P

V

I

N

G

G

G

Y

F

P

P

S

G

P

P

Q

Q

G

G

Q

P

Y

Y

C

C

S

G

V

I

G

G

A

A

T

E

N

K

A

S

V

F

G

G

R

R

Q

D

I

I

I

V

E

D

E

A

H

H

L

Y

D

K

T

A

C

C

I

L

E

Y

A

A

G

G

L

I

N

N

I

I

E
x
S

G

G

I

I

N

N

S

G

E
|
E

V

V

M

M

M

P

G

G

Q

Q

W

W

E
|
E

Y

F

Q
|
Q

I

V

F

G

S

P

K

S

N

V

A

G

T

I

E

S

A

S

C

G

D

D

Q

Q

I

V

W

W

A

V

S

A

R

R

Y

Y

F

I

L

L

E

E

R

R

I

I

T

T

E

E

Q

I

Y

A

N

G

V

V

K

V

V

V

D

T

W

F

D

D

P

P

K

K

P

P

V

I

Q

P

G

G

D

D

W

W

N

N

G
|
G

S

A

G

G

M

A

H
|
H

A

T

N
|
N

F
x
Y

S
|
S

N

T

K

E

Q

S

L

M

R

R

E

K

S

E

G

G

D

G

K

Y

K

E

I

V

F

I

D

K

K

A

I

A

C

I

E

E

E

K

F

L

K

K

L

L

T

R

H

H

K

K

E

E

H

H

I

I

A

A

V

A

Y

Y

G

G

D

E

H

G

N

N

E

E

Q

R

R
|
R

L

L

T

T

G

G

E

R

H

H

E
|
E

T

T

Q

A

D

D

I

I

N

N

S

T

F

F

S

S

Y

W

G

G

V

V

S

A

D

N

R
|
R

S

G

A

A

S

S

L

V

R
|
R

I

V

P

G

I

R

A

E

T

T

I

E

K

Q

N

N

N

G

W

-

K

K

G

G

R
x
Y

I

F

E
|
E

D

D

R
|
R

R

R

P

P

A

A

S

S

N

N

A

M

N

D

P

P

Y

Y

D

V

V

V

A

T

A

S

I

M

I

I

I

A

K

E

S

T

V

T

binding phosphoaminophosphonic acid-adenylate ester: G125 (= G105), E127 (= E107), S185 (≠ E165), E197 (= E177), Q199 (= Q179), N249 (= N229), Y250 (≠ F230), S251 (= S231), R309 (= R289), R314 (= R294), Y326 (≠ R307)
binding manganese (ii) ion: E127 (= E107), E127 (= E107), E129 (= E109), E190 (= E170), E197 (= E177), E197 (= E177), H247 (= H227), E328 (= E309)
binding (2s)-2-amino-4-(methylsulfonimidoyl)butanoic acid: E129 (= E109), E190 (= E170), G243 (= G223), H247 (= H227), R289 (= R269), E295 (= E275), R309 (= R289), R330 (= R311)

2d3aA Crystal structure of the maize glutamine synthetase complexed with adp and methionine sulfoximine phosphate (see paper)
44% identity, 98% coverage: 3:330/335 of query aligns to 16:349/353 of 2d3aA

query
sites
2d3aA

K

K

T

I

K

I

L

A

E

E

Y

Y

I

I

W

W

L

I

D

G

G

G

Y

S

K

G

P

-

T

-

Q

M

S

D

L

L

R

R

S

S

K

K

T

A

M

R

V

T

V

L

S

P

D

G

F

P

S

V

G

T

K

D

L

P

E

S

D

K

C

L

D

P

I

K

W

W

S

N

F

Y

D

D

G

G

S

S

T

T

K

G

Q

Q

A

A

V

P

G

G

N

E

N

D

S

S

D

E

C

V

L

I

L

L

K

Y

P

P

V

Q

A

A

L

I

Y

F

L

K

D

D

P

P

-

F

G

R

R

R

I

G

D

N

A

N

Y

I

I

L

V

V

I

M

N

C

E

D

V

C

L

Y

N

T

S

P

D

A

E

G

T

E

P

P

H

I

I

P

S

T

N

N

K

K

R

R

A

Y

T

S

I

A

E

A

E

K

-

I

-

F

-

S

-

P

-

E

-

V

-

A

N

A

D

E

F

P

W

W

F

Y

G
|
G

F

I

E
|
E

Q

Q

E
|
E

Y

Y

T

T

L

L

I

L

D

Q

L

K

E

D

T

T

N

N

R

W

P

P

I

L

G

G

F

W

P

P

V

I

N

G

G

G

Y

F

P

P

S

G

P

P

Q

Q

G

G

Q

P

Y

Y

C

C

S

G

V

I

G

G

A

A

T

E

N

K

A

S

V

F

G

G

R

R

Q

D

I

I

I

V

E

D

E

A

H

H

L

Y

D

K

T

A

C

C

I

L

E

Y

A

A

G

G

L

I

N

N

I

I

E
x
S

G

G

I

I

N

N

S

G

E
|
E

V

V

M

M

M

P

G

G

Q

Q

W

W

E
|
E

Y

F

Q
|
Q

I

V

F
x
G

S

P

K

S

N

V

A

G

T

I

E

S

A

S

C

G

D

D

Q

Q

I

V

W

W

A

V

S

A

R

R

Y

Y

F

I

L

L

E

E

R

R

I

I

T

T

E

E

Q

I

Y

A

N

G

V

V

K

V

V

V

D

T

W

F

D

D

P

P

K

K

P

P

V

I

Q

P

G

G

D

D

W

W

N

N

G
|
G

S

A

G

G

M

A

H
|
H

A

T

N
|
N

F

Y

S
|
S

N

T

K

E

Q

S

L

M

R

R

E

K

S

E

G

G

D

G

K

Y

K

E

I

V

F

I

D

K

K

A

I

A

C

I

E

E

E

K

F

L

K

K

L

L

T

R

H

H

K

K

E

E

H

H

I

I

A

A

V

A

Y

Y

G

G

D

E

H

G

N

N

E

E

Q

R

R
|
R

L

L

T

T

G

G

E

R

H

H

E
|
E

T

T

Q

A

D

D

I

I

N

N

S

T

F

F

S

S

Y

W

G

G

V

V

S

A

D

N

R
|
R

S

G

A

A

S

S

L

V

R
|
R

I

V

P

G

I

R

A

E

T

T

I

E

K

Q

N

N

N

G

W

-

K

K

G

G

R
x
Y

I

F

E
|
E

D

D

R
|
R

R

R

P

P

A

A

S

S

N

N

A

M

N

D

P

P

Y

Y

D

V

V

V

A

T

A

S

I

M

I

I

I

A

K

E

S

T

V

T

binding adenosine-5'-diphosphate: G125 (= G105), E127 (= E107), S185 (≠ E165), Q199 (= Q179), G201 (≠ F181), N249 (= N229), S251 (= S231), R314 (= R294), Y326 (≠ R307)
binding manganese (ii) ion: E127 (= E107), E127 (= E107), E129 (= E109), E190 (= E170), E197 (= E177), E197 (= E177), H247 (= H227), E328 (= E309)
binding l-methionine-s-sulfoximine phosphate: E127 (= E107), E129 (= E109), E190 (= E170), E197 (= E177), G243 (= G223), H247 (= H227), R289 (= R269), E295 (= E275), R309 (= R289), R330 (= R311)

P32288 Glutamine synthetase; GS; Glutamate--ammonia ligase; EC 6.3.1.2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 2 papers)
44% identity, 98% coverage: 7:335/335 of query aligns to 27:357/370 of P32288

query
sites
P32288

E

E

Y

Y

I

V

W

W

L

I

D

D

G

G

Y

-

K

-

P

-

T

T

Q

G

S

N

L

L

R

R

S

S

K

K

T

G

M

R

V

T

V

L

S

K

D

K

F

R

S

I

G

T

K

S

L

I

E

D

D

Q

C

L

D

P

I

E

W

W

S

N

F

F

D

D

G

G

S

S

T

T

K

N

Q

Q

A

A

V

P

G

G

N

H

N

D

S

S

D

D

C

I

L

Y

L

L

K

K

P

P

V

V

A

A

L

Y

Y

Y

L

P

D

D

P

P

-

F

G

R

R

R

I

G

D

D

A

N

Y

I

I

V

V

V

I

L

N

A

E

A

V

C

L

Y

N

N

S

N

D

D

E

G

T

T

P

P

-

N

-

K

-

F

-

N

-

H

-

R

H

H

I

E

S

A

N

A

K

K

R

L

A

F

T

A

I

A

E

H

E

K

N

D

D

E

F

I

W

W

F

F

G

G

F

L

E

E

Q

Q

E

E

Y

Y

T

T

L

L

I

F

D

D

L

M

E

Y

T

D

N

D

R

V

P

-

I

Y

G

G

F

W

P

P

V

K

N

G

G

G

Y

Y

P

P

S

A

P

P

Q

Q

G

G

Q

P

Y

Y

C

C

S

G

V

V

G

G

A

A

T

G

N

K

A

V

V

Y

G

A

R

R

Q

D

I

M

I

I

E

E

E

A

H

H

L

Y

D

R

T

A

C

C

I

L

E

Y

A

A

G

G

L

L

N

E

I

I

E

S

G

G

I

I

N

N

S

A

E

E

V

V

M

M

M

P

G

S

Q

Q

W

W

E

E

Y

F

Q

Q

I

V

F

G

S

P

K

C

N

T

A

G

T

I

E

D

A

M

C

G

D

D

Q

Q

I

L

W

W

A

M

S

A

R

R

Y

Y

F

F

L

L

E

H

R

R

I

V

T

A

E

E

Q

E

Y

F

N

G

V

I

K

K

V

I

D

S

W

F

D

H

P

P

K

K

P

P

V

L

Q

K

G

G

D

D

W

W

N

N

G

G

S

A

G

G

M

C

H

H

A

T

N

N

F

V

S

S

N

T

K

K

Q

E

L

M

R

R

E

Q

S

P

G

G

D

G

K

M

K

K

I

Y

F

I

D

E

K

Q

I

A

C

I

E

E

E

K

F

L

K

S

L

K

T

R

H

H

K

A

E

E

H

H

I

I

A

K

V

L

Y

Y

G

G

D

S

H

D

N

N

E

D

Q

M

R

R

L

L

T

T

G

G

E

R

H

H

E

E

T

T

Q

A

D

S

I

M

N

T

S

A

F

F

S

S

Y

S

G

G

V

V

S

A

D

N

R

R

S

G

A

S

S

S

L

I

R

R

I

I

P

P

I

R

A

S

T

V

I

A

K

K

N

E

N

G

W

Y

K

-

G

G

R

Y

I

F

E

E

D

D

R

R

P

P

A

A

S

S

N

N

A

I

N

D

P

P

Y

Y

D

L

V

V

A

T

A

G

I

I

I

M

I

C

K

E

S

T

V

V

K

C

K

G

A

A

L

I

N

D

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine

Q9FMD9 Glutamine synthetase cytosolic isozyme 1-4; Glutamate--ammonia ligase GLN1;4; GLN1;4; EC 6.3.1.2 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
45% identity, 97% coverage: 7:330/335 of query aligns to 22:351/356 of Q9FMD9

query
sites
Q9FMD9

E

E

Y

Y

I

I

W

W

L

I

D

G

G

G

Y

S

K

G

P

-

T

-

Q

L

S

D

L

M

R

R

S

S

K

K

T

A

M

R

V

T

V

L

S

P

D

G

F

P

S

V

G

T

K

D

L

P

E

S

D
x
Q

C

L

D

P

I

K

W

W

S

N

F

Y

D

D

G

G

S

S

T

T

K

G

Q

Q

A

A

V

P

G

G

N

D

S

S

D

E

C

V

L

I

K

Y

P

P

V

Q

A

A

L

I

Y

F

L

K

D

D

P

P

-

F

G

R

R

R

I

G

D

N

A

N

Y

I

I

L

V

V

I

M

N

C

E

D

V

A

L

Y

N

T

S

P

D

A

E

G

T

E

P

P

H

I

I

P

S

T

N

N

K

K

R

R

-

H

-

A

A

A

T

K

I

I

E

F

E

E

N

D

-

P

-

S

-

V

-

A

D

E

F

T

W

W

F

Y

G

G

F

I

E

E

Q

Q

E

E

Y

Y

T

T

L

L

I

L

D

Q

L

K

E

D

T

I

N

K

R

W

P

P

I

V

G

G

F

W

P

P

V

V

N

G

G

G

Y

F

P

P

S

G

P

P

Q

Q

G

G

Q

P

Y

Y

C

C

S

G

V

V

G

G

A

A

T

D

N

K

A

A

V

F

G

G

R

R

Q

D

I

I

I

V

E

D

E
x
S

H

H

L

Y

D

K

T

A

C

C

I

L

E

Y

A

A

G

G

L

I

N

N

I

V

E

S

G

G

I

T

N

N

S

G

E

E

V

V

M

M

M

P

G

G

Q

Q

W

W

E

E

Y

F

Q

Q

I

V

F

G

S

P

K

T

N

V

A

G

T

I

E

A

A

A

C

A

D

D

Q

Q

I

V

W

W

A

V

S

A

R

R

Y

Y

F

I

L

L

E

E

R

R

I

I

T

T

E

E

Q

L

Y

A

N

G

V

V

K

V

V

L

D

S

W

L

D

D

P

P

K

K

P

P

V

I

Q

P

G

G

D

D

W

W

N

N

G

G

S

A

G

G

M

A

H

H

A

T

N

N

F

Y

S

S

N

T

K

K

Q

S

L

M

R

R

E

E

S

D

G

G

D

G

K

Y

K

E

I

V

F

I

D

K

K

K

I

A

C

I

E

E

E

K

F

L

K

G

L

L

T

R

H

H

K

K

E

E

H

H

I

I

A

A

V

A

Y

Y

G

G

D

E

H

G

N

N

E

E

Q

R

R

R

L

L

T

T

G

G

E

K

H

H

E

E

T

T

Q

A

D

D

I

I

N

N

S

T

F

F

S

L

Y

W

G

G

V

V

S

A

D

N

R

R

S

G

A

A

S

S

L

I

R

R

I

V

P

G

I

R

A

D

T

T

I

E

K

Q

N

A

N

G

W

-

K

K

G

G

R

Y

I

F

E

E

D

D

R

R

P

P

A

A

S

S

N

N

A

M

N

D

P

P

Y

Y

D

T

V

V

A

T

A

S

I

M

I

I

I

A

K

E

S

S

V

T

Q49 (≠ D36) mutation to K: 6-fold decrease in affinity for ammonium and catalytic efficiency; when associated with A-174.
S174 (≠ E152) mutation to A: 6-fold decrease in affinity for ammonium and catalytic efficiency; when associated with K-49.

P15104 Glutamine synthetase; GS; Glutamate--ammonia ligase; Palmitoyltransferase GLUL; EC 6.3.1.2; EC 2.3.1.225 from Homo sapiens (Human) (see 4 papers)
43% identity, 98% coverage: 3:330/335 of query aligns to 25:359/373 of P15104

query
sites
P15104

K

K

T

V

K

Q

L

A

E

M

Y

Y

I

I

W

W

L

I

D

D

G

G

Y

T

K

G

P

-

T

-

Q

E

S

G

L

L

R

R

S

C

K

K

T

T

M

R

V

T

V

L

S

D

D

S

F

E

S

P

G

K

K

C

L

V

E

E

D

E

C

L

D

P

I

E

W

W

S

N

F

F

D

D

G

G

S

S

T

T

K

L

Q

Q

A

S

V

E

G

G

N

S

N

N

S

S

D

D

C

M

L

Y

L

L

K

V

P

P

V

A

A

A

L

M

Y

F

L

R

D

D

P

P

G

F

R

R

I

K

D

D

A

P

-

N

Y

K

I

L

V

V

I

L

N

C

E

E

V

V

L

F

N

K

S

Y

D

N

E

R

T

R

P

P

H

A

I

E

S

T

N

N

K

L

R

R

A

H

T

T

I

C

E

K

E

R

-

I

-

M

-

D

-

M

-

V

-

S

N

N

D

Q

D

H

F

P

W

W

F

F

G

G

F

M

E

E

Q

Q

E

E

Y

Y

T

T

L

L

I

M

D

G

L

T

E

D

T

-

N

G

R

H

P

P

I

F

G

G

F

W

P

P

V

S

N

N

G

G

Y

F

P

P

S

G

P

P

Q

Q

G

G

Q

P

Y

Y

C

C

S

G

V

V

G

G

A

A

T

D

N

R

A

A

V

Y

G

G

R

R

Q

D

I

I

I

V

E

E

E

A

H

H

L

Y

D

R

T

A

C

C

I

L

E

Y

A

A

G

G

L

V

N

K

I

I

E

A

G

G

I

T

N

N

S

A

E

E

V

V

M

M

M

P

G

A

Q

Q

W

W

E

E

Y

F

Q

Q

I

I

F

G

S

P

K
x
C

N

E

A

G

T

I

E

S

A

M

C

G

D

D

Q

H

I

L

W

W

A

V

S

A

R

R

Y

F

F

I

L

L

E

H

R

R

I

V

T

C

E

E

Q

D

Y

F

N

G

V

V

K

I

V

A

D

T

W

F

D

D

P

P

K

K

P

P

V

I

Q

P

G

G

D

N

W

W

N

N

G

G

S

A

G

G

M

C

H

H

A

T

N

N

F

F

S

S

N

T

K

K

Q

A

L

M

R

R

E

E

S

E

G

N

D

G

K

L

K

K

I

Y

F

I

D

E

K

E

I

A

C

I

E

E

E

K

F

L

K

S

L

K

T

R

H

H

K

Q

E

Y

H

H

I

I

A

R

V

A

Y

Y

-

D

-

P

-

K

-

G

G

G

D

L

H

D

N

N

E

A

Q

R

R
|
R

L

L

T

T

G

G

E

F

H

H

E

E

T

T

Q

S

D

N

I

I

N

N

S

D

F

F

S

S

Y

A

G

G

V

V

S

A

D

N

R

R

S

S

A

A

S

S

L

I

R
|
R

I

I

P

P

I

-

A

R

T

T

I

V

K

G

N

Q

N

E

W

K

K

K

G

G

R

Y

I

F

E

E

D

D

R

R

R
|
R

P

P

A

S

S

A

N

N

A

C

N

D

P

P

Y

F

D

S

V

V

A

T

A

E

I

A

I

L

I

I

K

R

S

T

V

C

C209 (≠ K183) mutation to A: Reduced ability to mediate autopalmitoylation.
R299 (= R269) mutation to E: Loss glutamine synthase activity. Does not affect interaction with BEST2.
R324 (= R294) to C: in CSGD; reduced glutamine synthetase activity; dbSNP:rs80358214; mutation R->A,C: Decreases ribolosomal 40S subunit synthesis. Loss of nucleolar location of BYSL.
R341 (= R312) to C: in CSGD; suggests reduced glutamine synthetase activity; dbSNP:rs80358215

2qc8A Crystal structure of human glutamine synthetase in complex with adp and methionine sulfoximine phosphate (see paper)
43% identity, 98% coverage: 3:330/335 of query aligns to 16:350/356 of 2qc8A

query
sites
2qc8A

K

K

T

V

K

Q

L

A

E

M

Y

Y

I

I

W

W

L

I

D

D

G

G

Y

T

K

G

P

-

T

-

Q

E

S

G

L

L

R

R

S

C

K

K

T

T

M

R

V

T

V

L

S

D

D

S

F

E

S

P

G

K

K

C

L

V

E

E

D

E

C

L

D

P

I

E

W

W

S

N

F

F

D

D

G

G

S

S

T

T

K

L

Q

Q

A

S

V

E

G

G

N

S

N

N

S

S

D

D

C

M

L

Y

L

L

K

V

P

P

V

A

A

A

L

M

Y

F

L

R

D

D

P

P

G

F

R

R

I

K

D

D

A

P

-

N

Y

K

I

L

V

V

I

L

N

C

E

E

V

V

L

F

N

K

S

Y

D

N

E

R

T

R

P

P

H

A

I

E

S

T

N

N

K

L

R

R

A

H

T

T

I

C

E

K

E

R

-

I

-

M

-

D

-

M

-

V

-

S

N

N

D

Q

D

H

F

P

W
|
W

F

F

G
|
G

F

M

E
|
E

Q

Q

E
|
E

Y

Y

T

T

L

L

I

M

D

G

L

T

E

D

T

-

N

G

R

H

P

P

I

F

G

G

F

W

P

P

V

S

N

N

G

G

Y

F

P

P

S

G

P

P

Q

Q

G

G

Q

P

Y

Y

C

C

S

G

V

V

G

G

A

A

T

D

N

R

A

A

V

Y

G

G

R

R

Q

D

I

I

I

V

E

E

E

A

H

H

L

Y

D

R

T

A

C

C

I

L

E

Y

A

A

G

G

L

V

N

K

I

I

E

A

G

G

I

T

N

N

S

A

E
|
E

V

V

M

M

M

P

G

A

Q

Q

W

W

E
|
E

Y

F

Q
|
Q

I

I

F
x
G

S
x
P

K

C

N

E

A

G

T

I

E

S

A

M

C

G

D

D

Q

H

I

L

W

W

A

V

S

A

R

R

Y

F

F

I

L

L

E

H

R

R

I

V

T

C

E

E

Q

D

Y

F

N

G

V

V

K

I

V

A

D

T

W

F

D

D

P

P

K

K

P

P

V

I

Q

P

G

G

D

N

W

W

N

N

G
|
G

S

A

G

G

M

C

H
|
H

A

T

N
|
N

F

F

S
|
S

N

T

K

K

Q

A

L

M

R

R

E

E

S

E

G

N

D

G

K

L

K

K

I

Y

F

I

D

E

K

E

I

A

C

I

E

E

E

K

F

L

K

S

L

K

T

R

H

H

K

Q

E

Y

H

H

I

I

A

R

V

A

Y

Y

-

D

-

P

-

K

-

G

G

G

D

L

H

D

N

N

E

A

Q

R

R
|
R

L

L

T

T

G

G

E

F

H

H

E
|
E

T

T

Q

S

D

N

I

I

N

N

S

D

F

F

S

S

Y

A

G

G

V

V

S

A

D

N

R
|
R

S

S

A

A

S

S

L

I

R
|
R

I

I

P

P

I

-

A

R

T

T

I

V

K

G

N

Q

N

E

W

K

K

K

G

G

R
x
Y

I

F

E
|
E

D

D

R
|
R

R

R

P

P

A

S

S

A

N

N

A

C

N

D

P

P

Y

F

D

S

V

V

A

T

A

E

I

A

I

L

I

I

K

R

S

T

V

C

binding adenosine-5'-diphosphate: W121 (= W103), G123 (= G105), E125 (= E107), Q196 (= Q179), G198 (≠ F181), P199 (≠ S182), N246 (= N229), S248 (= S231), R315 (= R294), Y327 (≠ R307), E329 (= E309)
binding manganese (ii) ion: E125 (= E107), E125 (= E107), E127 (= E109), E187 (= E170), E194 (= E177), E194 (= E177), H244 (= H227), E329 (= E309)
binding l-methionine-s-sulfoximine phosphate: E125 (= E107), E127 (= E109), E187 (= E170), E194 (= E177), G240 (= G223), H244 (= H227), R290 (= R269), E296 (= E275), R310 (= R289), R331 (= R311)

Q8HZM5 Glutamine synthetase; GS; Glutamate--ammonia ligase; Palmitoyltransferase GLUL; EC 6.3.1.2; EC 2.3.1.225 from Canis lupus familiaris (Dog) (Canis familiaris) (see paper)
42% identity, 98% coverage: 3:330/335 of query aligns to 25:359/373 of Q8HZM5

query
sites
Q8HZM5

K

K

T

V

K

Q

L

A

E

M

Y

Y

I

I

W

W

L

I

D

D

G

G

Y

T

K

-

P

-

T

G

Q

E

S

G

L

L

R

R

S

C

K

K

T

T

M

R

V

T

V

L

S

D

D

S

F

E

S

P

G

K

K

G

L

V

E

E

D

E

C

L

D

P

I

E

W

W

S

N

F

F

D

D

G

G

S

S

T

T

K

F

Q

Q

A

S

V

E

G

G

N

S

N

N

S

S

D

D

C

M

L

Y

L

L

K

V

P

P

V

A

A

A

L

M

Y

F

L

R

D

D

P

P

G

F

R

R

I

K

D

D

A

P

-

N

Y

K

I

L

V

V

I

F

N

C

E

E

V

V

L

F

N

K

S

Y

D

N

E

R

T

K

P

P

H

A

I

E

S

T

N

N

K

L

R

R

A

H

T

T

I

C

E

K

E

R

-

I

-

M

-

D

-

M

-

V

-

S

N

N

D

Q

D

H

F

P

W

W

F

F

G

G

F

M

E

E

Q

Q

E
|
E

Y

Y

T

T

L

L

I

M

D

G

L

T

E

D

T

-

N

G

R

H

P

P

I

F

G

G

F

W

P

P

V

S

N

N

G

G

Y

F

P

P

S

G

P

P

Q

Q

G

G

Q

P

Y

Y

C

C

S

G

V

V

G

G

A

A

T

D

N

K

A

A

V

Y

G

G

R

R

Q

D

I

I

I

V

E

E

E

A

H

H

L

Y

D

R

T

A

C

C

I

L

E

Y

A

A

G

G

L

I

N

K

I

I

E

A

G

G

I

T

N

N

S

A

E
|
E

V

V

M

M

M

P

G

A

Q

Q

W

W

E
|
E

Y

F

Q

Q

I

I

F

G

S

P

K

C

N

E

A

G

T

I

E

D

A

M

C

G

D

D

Q

H

I

L

W

W

A

V

S

A

R

R

Y

F

F

I

L

L

E

H

R

R

I

V

T

C

E

E

Q

D

Y

F

N

G

V

V

K

I

V

A

D

T

W

F

D

D

P

P

K

K

P

P

V

I

Q

P

G

G

D

N

W

W

N

N

G

G

S

A

G

G

M

C

H

H

A

T

N

N

F

F

S

S

N

T

K

K

Q

A

L

M

R

R

E

E

S

E

G

N

D

G

K

L

K

K

I

Y

F

I

D

E

K

E

I

S

C

I

E

E

E

K

F

L

K

S

L

K

T

R

H

H

K

Q

E

Y

H

H

I

I

A

R

V

A

Y

Y

-

D

-

P

-

K

-

G

G

G

D

L

H

D

N

N

E

A

Q

R

R

R

L

L

T

T

G

G

E

F

H

H

E

E

T

T

Q

S

D

N

I

I

N

N

S

D

F

F

S

S

Y

A

G

G

V

V

S

A

D

N

R

R

S

G

A

A

S

S

L

I

R

R

I

I

P

P

I

-

A

R

T

T

I

V

K

G

N

Q

N

E

W

K

K

K

G

G

R

Y

I

F

E

E

D

D

R

R

P

P

A

S

S

A

N

N

A

C

N

D

P

P

Y

F

D

S

V

V

A

T

A

E

I

A

I

L

I

I

K

R

S

T

V

C

E136 (= E109) binding Mn(2+)
E196 (= E170) binding Mn(2+)
E203 (= E177) binding Mn(2+)

P15105 Glutamine synthetase; GS; Glutamate--ammonia ligase; Palmitoyltransferase GLUL; EC 6.3.1.2; EC 2.3.1.225 from Mus musculus (Mouse)
42% identity, 98% coverage: 3:330/335 of query aligns to 25:359/373 of P15105

query
sites
P15105

K

K

T

V

K

Q

L

A

E

M

Y

Y

I

I

W

W

L

V

D

D

G

G

Y

T

K

-

P

-

T

G

Q

E

S

G

L

L

R

R

S

C

K

K

T

T

M

R

V

T

V

L

S

D

D

C

F

E

S

P

G

K

K

C

L

V

E

E

D

E

C

L

D

P

I

E

W

W

S

N

F

F

D

D

G

G

S

S

T

T

K

F

Q

Q

A

S

V

E

G

G

N

S

N

N

S

S

D

D

C

M

L

Y

L

L

K

H

P

P

V

V

A

A

L

M

Y

F

L

R

D

D

P

P

G

F

R

R

I

K

D

D

A

P

-

N

Y

K

I

L

V

V

I

L

N

C

E

E

V

V

L

F

N

K

S

Y

D

N

E

R

T

K

P

P

-

A

-

E

-

T

-

N

-

L

-

R

H

H

I

I

S

C

N

K

K

R

R

I

A

M

T

D

I

M

E

V

E

S

N

N

D

Q

D

H

F

P

W

W

F

F

G

G

F

M

E

E

Q

Q

E

E

Y

Y

T

T

L

L

I

M

D

G

L

T

E

D

T

-

N

G

R

H

P

P

I

F

G

G

F

W

P

P

V

S

N

N

G

G

Y

F

P

P

S

G

P

P

Q

Q

G

G

Q

P

Y

Y

C

C

S

G

V

V

G

G

A

A

T

D

N

K

A

A

V

Y

G

G

R

R

Q

D

I

I

I

V

E

E

E

A

H

H

L

Y

D

R

T

A

C

C

I

L

E

Y

A

A

G

G

L

V

N

K

I

I

E

T

G

G

I

T

N

N

S

A

E

E

V

V

M

M

M

P

G

A

Q

Q

W

W

E

E

Y

F

Q

Q

I

I

F

G

S

P

K

C

N

E

A

G

T

I

E

R

A

M

C

G

D

D

Q

H

I

L

W

W

A

I

S

A

R

R

Y

F

F

I

L

L

E

H

R

R

I

V

T

C

E

E

Q

D

Y

F

N

G

V

V

K

I

V

A

D

T

W

F

D

D

P

P

K

K

P

P

V

I

Q

P

G

G

D

N

W

W

N

N

G

G

S

A

G

G

M

C

H

H

A

T

N

N

F

F

S

S

N

T

K

K

Q

A

L

M

R

R

E

E

S

E

G

N

D

G

K

L

K

K

I

C

F

I

D

E

K

E

I

A

C

I

E

D

E

K

F

L

K

S

L

K

T

R

H

H

K

Q

E

Y

H

H

I

I

A

R

V

A

Y

Y

-

D

-

P

-

K

-

G

G

G

D

L

H

D

N

N

E

A

Q

R

R

R

L

L

T

T

G

G

E

F

H

H

E

E

T

T

Q

S

D

N

I

I

N

N

S

D

F

F

S

S

Y

A

G

G

V

V

S

A

D

N

R

R

S

G

A

A

S

S

L

I

R

R

I

I

P

P

I

-

A

R

T

T

I

V

K

G

N

Q

N

E

W

K

K

K

G

G

R

Y

I

F

E

E

D

D

R

R

P

P

A

S

S

A

N

N

A

C

N

D

P

P

Y

Y

D

A

V

V

A

T

A

E

I

A

I

I

I

V

K

R

S

T

V

C

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine

2uu7A Crystal structure of apo glutamine synthetase from dog (canis familiaris) (see paper)
42% identity, 98% coverage: 3:330/335 of query aligns to 23:357/370 of 2uu7A

query
sites
2uu7A

K

K

T

V

K

Q

L

A

E

M

Y

Y

I

I

W

W

L

I

D

D

G

G

Y

T

K

-

P

-

T

G

Q

E

S

G

L

L

R

R

S

C

K

K

T

T

M

R

V

T

V

L

S

D

D

S

F

E

S

P

G

K

K

G

L

V

E

E

D

E

C

L

D

P

I

E

W

W

S

N

F

F

D

D

G

G

S

S

T

T

K

F

Q

Q

A

S

V

E

G

G

N

S

N

N

S

S

D

D

C

M

L

Y

L

L

K

V

P

P

V

A

A

A

L

M

Y

F

L

R

D

D

P

P

G

F

R

R

I

K

D

D

A

P

-

N

Y

K

I

L

V

V

I

F

N

C

E

E

V

V

L

F

N

K

S

Y

D

N

E

R

T

K

P

P

H

A

I

E

S

T

N

N

K

L

R

R

A

H

T

T

I

C

E

K

E

R

-

I

-

M

-

D

-

M

-

V

-

S

N

N

D

Q

D

H

F

P

W

W

F

F

G

G

F

M

E

E

Q

Q

E
|
E

Y

Y

T

T

L

L

I

M

D

G

L

T

E

D

T

-

N

G

R

H

P

P

I

F

G

G

F

W

P

P

V

S

N

N

G

G

Y

F

P

P

S

G

P

P

Q

Q

G

G

Q

P

Y

Y

C

C

S

G

V

V

G

G

A

A

T

D

N

K

A

A

V

Y

G

G

R

R

Q

D

I

I

I

V

E

E

E

A

H

H

L

Y

D

R

T

A

C

C

I

L

E

Y

A

A

G

G

L

I

N

K

I

I

E

A

G

G

I

T

N

N

S

A

E
|
E

V

V

M

M

M

P

G

A

Q

Q

W

W

E
|
E

Y

F

Q

Q

I

I

F

G

S

P

K

C

N

E

A

G

T

I

E

D

A

M

C

G

D

D

Q

H

I

L

W

W

A

V

S

A

R

R

Y

F

F

I

L

L

E

H

R

R

I

V

T

C

E

E

Q

D

Y

F

N

G

V

V

K

I

V

A

D

T

W

F

D

D

P

P

K

K

P

P

V

I

Q

P

G

G

D

N

W

W

N

N

G

G

S

A

G

G

M

C

H

H

A

T

N

N

F

F

S

S

N

T

K

K

Q

A

L

M

R

R

E

E

S

E

G

N

D

G

K

L

K

K

I

Y

F

I

D

E

K

E

I

S

C

I

E

E

E

K

F

L

K

S

L

K

T

R

H

H

K

Q

E

Y

H

H

I

I

A

R

V

A

Y

Y

-

D

-

P

-

K

-

G

G

G

D

L

H

D

N

N

E

A

Q

R

R

R

L

L

T

T

G

G

E

F

H

H

E

E

T

T

Q

S

D

N

I

I

N

N

S

D

F

F

S

S

Y

A

G

G

V

V

S

A

D

N

R

R

S

G

A

A

S

S

L

I

R

R

I

I

P

P

I

-

A

R

T

T

I

V

K

G

N

Q

N

E

W

K

K

K

G

G

R

Y

I

F

E

E

D

D

R

R

P

P

A

S

S

A

N

N

A

C

N

D

P

P

Y

F

D

S

V

V

A

T

A

E

I

A

I

L

I

I

K

R

S

T

V

C

binding magnesium ion: E134 (= E109), E194 (= E170), E201 (= E177)

8ecyA Cryoem structure of bovine bestrophin-2 and glutamine synthetase complex (see paper)
42% identity, 98% coverage: 3:330/335 of query aligns to 24:358/371 of 8ecyA

query
sites
8ecyA

K

K

T

V

K

Q

L

A

E

M

Y

Y

I

I

W

W

L

I

D

D

G

G

Y

T

K

-

P

-

T

G

Q

E

S

G

L

L

R

R

S

C

K

K

T

T

M

R

V

T

V

L

S

D

D

S

F

E

S

P

G

K

K

C

L

I

E

E

D

E

C

L

D

P

I

E

W

W

S

N

F

F

D

D

G

G

S

S

T

T

K

F

Q

Q

A

S

V

E

G

G

N

S

N

N

S

S

D

D

C

M

L

Y

L

L

K

V

P

P

V

A

A

A

L

M

Y

F

L

R

D

D

P

P

G

F

R

R

I

K

D

D

A

P

-

N

Y

K

I

L

V

V

I

F

N

C

E

E

V

V

L

F

N

K

S

Y

D

N

E

R

T

K

P

P

H

A

I

E

S

T

N

N

K

L

R

R

A

H

T

T

I

C

E

K

E

R

-

I

-

M

-

D

-

M

-

V

-

S

N

N

D

Q

D

R

F

P

W

W

F

F

G

G

F

M

E
|
E

Q

Q

E
|
E

Y

Y

T

T

L

L

I

M

D

G

L

T

E

D

T

-

N

G

R

H

P

P

I

F

G

G

F

W

P

P

V

S

N

N

G

G

Y

F

P

P

S

G

P

P

Q

Q

G

G

Q

P

Y

Y

C

C

S

G

V

V

G

G

A

A

T

D

N

K

A

A

V

Y

G

G

R

R

Q

D

I

I

I

V

E

E

E

A

H

H

L

Y

D

R

T

A

C

C

I

L

E

Y

A

A

G

G

L

I

N

K

I

I

E

G

G

G

I

T

N

N

S

A

E
|
E

V

V

M

M

M

P

G

A

Q

Q

W

W

E
|
E

Y

F

Q

Q

I

I

F

G

S

P

K

C

N

E

A

G

T

I

E

D

A

M

C

G

D

D

Q

H

I

L

W

W

A

V

S

A

R

R

Y

F

F

I

L

L

E

H

R

R

I

V

T

C

E

E

Q

D

Y

F

N

G

V

V

K

I

V

A

D

T

W

F

D

D

P

P

K

K

P

P

V

I

Q

P

G

G

D

N

W

W

N

N

G

G

S

A

G

G

M

C

H

H

A

T

N

N

F

F

S

S

N

T

K

K

Q

A

L

M

R

R

E

E

S

E

G

N

D

G

K

L

K

K

I

Y

F

I

D

E

K

E

I

A

C

I

E

E

E

K

F

L

K

S

L

K

T

R

H

H

K

Q

E

Y

H

H

I

I

A

R

V

A

Y

Y

-

D

-

P

-

K

-

G

G

G

D

L

H

D

N

N

E

A

Q

R

R

R

L

L

T

T

G

G

E

F

H

H

E

E

T

T

Q

S

D

N

I

I

N

N

S

D

F

F

S

S

Y

A

G

G

V

V

S

A

D

N

R

R

S

G

A

A

S

S

L

I

R

R

I

I

P

P

I

-

A

R

T

T

I

V

K

G

N

Q

N

E

W

K

K

K

G

G

R

Y

I

F

E
|
E

D

D

R

R

P

P

A

S

S

A

N

N

A

C

N

D

P

P

Y

F

D

A

V

V

A

T

A

E

I

A

I

L

I

I

K

R

S

T

V

C

binding manganese (ii) ion: E133 (= E107), E135 (= E109), E195 (= E170), E202 (= E177), E337 (= E309)

7cprA Glutamine synthetase from drosophila (see paper)
41% identity, 96% coverage: 8:330/335 of query aligns to 34:363/367 of 7cprA

query
sites
7cprA

Y

Y

I

V

W

W

L

I

D

D

G

G

Y

T

K

G

P

-

T

-

Q

E

S

D

L

L

R

R

S

C

K

K

T

D

M

R

V

T

V

L

S

D

D

F

F

I

S

P

G

Q

K

S

L

P

E

K

D

E

C

L

D

P

I

V

W

W

S

N

F

Y

D

D

G

G

S

S

T

C

K

Y

Q

Q

A

A

V

E

G

G

N

S

N

N

S

S

D

D

C

T

L

Y

L

L

K

Y

P

P

V

V

A

A

L

I

Y

Y

L

K

D

D

P

P

-

F

G

R

R

R

I

G

D

N

A

N

Y

I

I

L

V

V

I

M

N

C

E

D

V

T

L

Y

N

K

S

F

D

D

E

G

T

T

P

P

H

T

I

D

S

T

N

N

K

K

R

R

A

K

T

T

I

C

E

L

E

E

-

V

-

A

-

N

-

K

-

C

-

A

N

A

D

E

F

P

W

W

F

F

G

G

F

I

E
|
E

Q

Q

E

E

Y

Y

T

T

L

F

I

L

D

D

L

F

E

D

T

-

N

G

R

H

P

P

I

L

G

G

F

W

P

P

V

K

N

N

G

G

Y

F

P

P

S

G

P

P

Q

Q

G

G

Q

P

Y

Y

C

C

S

G

V

V

G

G

A

A

T

N

N

K

A

V

V

Y

G

A

R

R

Q

D

I

I

I

V

E

D

E

A

H

H

L

Y

D

R

T

A

C

C

I

L

E

Y

A

A

G

G

L

I

N

K

I

V

E
x
S

G

G

I

T

N

N

S

A

E

E

V

V

M

M

M

P

G

A

Q

Q

W

W

E

E

Y

F

Q
|
Q

I
x
V

F
x
G

S
x
P

K

C

N

E

A

G

T

I

E

S

A

I

C

G

D

D

Q

D

I

L

W

W

A

M

S

A

R

R

Y

F

F

L

L

L

E

H

R

R

I

I

T

S

E

E

Q

E

Y

F

N

G

V

I

K

V

V

S

D

T

W

L

D

D

P

P

K

K

P

P

V

M

Q

P

G

G

D

D

W

W

N

N

G

G

S

A

G

G

M

A

H

H

A

T

N
|
N

F

V

S
|
S

N

T

K

K

Q

A

L

M

R

R

E

E

S

D

G

G

D

G

K

I

K

R

I

D

F

I

D

E

K

K

I

A

C

V

E

A

E

K

F

L

K

S

L

K

T

C

H

H

K

E

E

R

H

H

I

I

A

R

V

A

Y

Y

-

D

-

P

-

K

-

Q

G

G

D

Q

H

D

N

N

E

A

Q

R

R

R

L

L

T

T

G

G

E

K

H

H

E

E

T

T

Q

S

D

S

I

I

N

N

S

D

F

F

S

S

Y

A

G

G

V

V

S

A

D

N

R

R

S

G

A

C

S

S

L

I

R

R

I

I

P

P

I

R

A

G

T

-

I

V

K

N

N

D

W

G

K

K

G

G

R
x
Y

I

F

E

E

D

D

R

R

P

P

A

S

S

S

N

N

A

C

N

D

P

P

Y

Y

D

S

V

V

A

V

A

E

I

A

I

I

I

L

K

R

S

T

V

I

binding adenosine-5'-diphosphate: E138 (= E107), S195 (≠ E165), Q209 (= Q179), V210 (≠ I180), G211 (≠ F181), P212 (≠ S182), N259 (= N229), S261 (= S231), Y340 (≠ R307)

2ojwA Crystal structure of human glutamine synthetase in complex with adp and phosphate (see paper)
42% identity, 98% coverage: 3:330/335 of query aligns to 26:354/360 of 2ojwA

query
sites
2ojwA

K

K

T

V

K

Q

L

A

E

M

Y

Y

I

I

W

W

L

I

D

D

G

G

Y

T

K

-

P

-

T

G

Q

E

S

G

L

L

R

R

S

C

K

K

T

T

M

R

V

T

V

L

S

D

D

S

F

E

S

P

G

K

K

C

L

V

E

E

D

E

C

L

D

P

I

E

W

W

S

N

F

F

D

D

G

G

S

S

T

T

K

L

Q

Q

A

S

V

E

G

G

N

S

N

N

S

S

D

D

C

M

L

Y

L

L

K

V

P

P

V

A

A

A

L

M

Y

F

L

R

D

D

P

P

G

F

R

R

I

K

D

D

A

P

-

N

Y

K

I

L

V

V

I

L

N

C

E

E

V

V

L

F

N

K

S

Y

D

N

E

R

T

R

P

P

H

A

I

E

S

T

N

N

K

L

R

R

A

H

T

T

I

C

E

K

E

R

-

I

-

M

-

D

-

M

-

V

-

S

N

N

D

Q

D

H

F

P

W
|
W

F

F

G

G

F

M

E
|
E

Q

Q

E
|
E

Y

Y

T

T

L

L

I

M

D

G

L

-

E

-

T

D

N

G

R

H

P

P

I

F

G

G

F

W

P

P

V

S

N

N

G

G

Y

F

P

P

S

G

P

P

Q

Q

G

G

Q

P

Y

Y

C

C

S

G

V

V

G

G

A

A

T

D

N

R

A

A

V

Y

G

G

R

R

Q

D

I

I

I

V

E

E

E

A

H

H

L

Y

D

R

T

A

C

C

I

L

E

Y

A

A

G

G

L

V

N

K

I

I

E

A

G

G

I

T

N

N

S

A

E
|
E

V

V

M

M

M

P

G

A

Q

Q

W

W

E
|
E

Y

F

Q
|
Q

I

I

F
x
G

S
x
P

K

C

N

E

A

G

T

I

E

S

A

M

C

G

D

D

Q

H

I

L

W

W

A

V

S

A

R

R

Y

F

F

I

L

L

E

H

R

R

I

V

T

C

E

E

Q

D

Y

F

N

G

V

V

K

I

V

A

D

T

W

F

D

D

P

P

K

K

P

P

V

I

Q

P

G

G

D

N

W

W

N

N

G

G

S

A

G

G

M

C

H
|
H

A

T

N
|
N

F

F

S
|
S

N

T

K

K

Q

A

L

M

R

R

E

E

S

E

G

N

D

G

K

L

K

K

I

Y

F

I

D

E

K

E

I

A

C

I

E

E

E

K

F

L

K

S

L

K

T

R

H

H

K

Q

E

Y

H

H

I

I

A

R

V

A

Y

Y

-

D

-

P

-

K

-

G

G

G

D

L

H

D

N

N

E

A

Q

R

R

R

L

L

T

-

G

-

E

-

H

-

E

-

T

T

Q

S

D

N

I

I

N

N

S

D

F

F

S

S

Y

A

G

G

V

V

S

A

D

N

R
|
R

S

S

A

A

S

S

L

I

R
|
R

I

I

P

P

I

-

A

R

T

T

I

V

K

G

N

Q

N

E

W

K

K

K

G

G

R
x
Y

I

F

E
|
E

D

D

R

R

P

P

A

S

S

A

N

N

A

C

N

D

P

P

Y

F

D

S

V

V

A

T

A

E

I

A

I

L

I

I

K

R

S

T

V

C

binding adenosine-5'-diphosphate: W131 (= W103), E135 (= E107), Q205 (= Q179), G207 (≠ F181), P208 (≠ S182), N255 (= N229), S257 (= S231), R319 (= R294), Y331 (≠ R307)
binding manganese (ii) ion: E135 (= E107), E135 (= E107), E137 (= E109), E196 (= E170), E203 (= E177), E203 (= E177), H253 (= H227), E333 (= E309)
binding phosphate ion: E135 (= E107), E203 (= E177), H253 (= H227), R314 (= R289)

7u5nA Cryo-em structure of glutamine synthetase (see paper)
38% identity, 98% coverage: 3:330/335 of query aligns to 24:330/343 of 7u5nA

query
sites
7u5nA

K

K

T

V

K

Q

L

A

E

M

Y

Y

I

I

W

W

L

I

D

D

G

G

Y

T

K

-

P

-

T

G

Q

E

S

G

L

L

R

R

S

C

K

K

T

T

M

R

V

T

V

L

S

D

D

S

F

E

S

P

G

K

K

C

L

I

E

E

D

E

C

L

D

P

I

E

W

W

S

N

F

F

D

D

G

G

S

S

T

T

K

F

Q

Q

A

S

V

E

G

G

N

S

N

N

S

S

D

D

C

M

L

Y

L

L

K

V

P

P

V

A

A

A

L

M

Y

F

L

R

D

D

P

P

G

F

R

R

I

K

D

D

A

P

-

N

Y

K

I

L

V

V

I

F

N

C

E

E

V

V

L

F

N

K

S

Y

D

N

E

R

T

K

P

P

H

A

I

E

S

T

N

N

K

L

R

R

A

H

T

T

I

C

E

K

E

R

-

I

-

M

-

D

-

M

-

V

-

S

N

N

D

Q

D

R

F

P

W

W

F

F

G

G

F

M

E
|
E

Q

Q

E

E

Y

Y

T

T

L

L

I

M

D

G

L

T

E

D

T

-

N

G

R

H

P

P

I

F

G

G

F

W

P

P

V

S

N

N

G

G

Y

F

P

P

S

G

P

P

Q

Q

G

G

Q

P

Y

Y

C

C

S

G

V

V

G

G

A

A

T

D

N

K

A

A

V

Y

G

G

R

R

Q

D

I

I

I

V

E

E

E

A

H

H

L

Y

D

R

T

A

C

C

I

L

E

Y

A

A

G

G

L

I

N

K

I

I

E

G

G

G

I

T

N

N

S

A

E

E

V

V

M

M

M

P

G

A

Q

Q

W

W

E

E

Y

F

Q

Q

I

I

F

G

S

P

K

C

N

E

A

G

T

I

E

D

A

M

C

G

D

D

Q

H

I

L

W

W

A

V

S

A

R

R

Y

F

F

I

L

L

E

H

R

R

I

V

T

C

E

E

Q

D

Y

F

N

G

V

V

K

I

V

A

D

T

W

F

D

D

P

P

K

K

P

P

V

I

Q

P

G

G

D

N

W

W

N

N

G

G

S

A

G

G

M

C

H
|
H

A

T

N

N

F

F

S

S

N

T

K

K

Q

A

L

M

R

R

E

E

S

E

G

N

D

G

K

L

K

K

I

Y

F

I

D

E

K

E

I

A

C

I

E

E

E

K

F

L

K

S

L

K

T

R

H

H

K

Q

E

Y

H

H

I

I

A

R

V

A

Y

Y

G

-

D

-

H

-

N

-

E

-

Q

-

R

-

L

-

T

-

G

-

E

-

H

-

E

-

T

-

Q

-

D

-

I

-

N

-

S

-

F

-

S

-

Y

-

G

-

V

-

S

D

D

P

R

K

S

G

A

A

S

S

L

I

R

R

I

I

P

P

I

-

A

R

T

T

I

V

K

G

N

Q

N

E

W

K

K

K

G

G

R

Y

I

F

E
|
E

D

D

R
|
R

R

R

P

P

A

S

S

A

N

N

A

C

N

D

P

P

Y

F

D

A

V

V

A

T

A

E

I

A

I

L

I

I

K

R

S

T

V

C

binding manganese (ii) ion: E133 (= E107), H252 (= H227), E309 (= E309), R311 (= R311)

8oooA Glutamine synthetase from methanothermococcus thermolithotrophicus in complex with 2-oxoglutarate and mgatp at 2.15 a resolution (see paper)
26% identity, 86% coverage: 42:329/335 of query aligns to 56:357/447 of 8oooA

query
sites
8oooA

F

F

D

D

G

G

S

S

T

I

K

E

Q

G

A

F

V

V

G

G

-

I

N

N

N

E

S

S

D

D

C

M

L

M

L

L

K

K

P

P

-

D

-

L

-

S

-

T

-

F

V

S

A

V

L

L

Y

P

L

W

D
x
R

P

P

G

S

R

E

I

K

D

S

A
x
V

Y

A

I

R

V

V

I

I

N

C

E

D

V

V

L

Y

N

T

S

T

D

K

E

G

T

K

P

P

H

F

I

E

S

G

N

D

K

P

R

R

A

G

T

C

I

L

-

K

-

R

-

V

-

M

-

E

-

F

-

K

E

E

E

F

N

N

D

G

D

E

F

Y

W

F

F

V

G

G

F

P

E
|
E

Q

P

E

E

Y

F

T

F

L

L

I

L

D

K

L

K

E

D

T

P

N

H

R

N

P

P

I

H

G

K

F

Y

P

-

V

-

N

-

G

-

Y

I

P

P

S

A

P

D

Q

D

G

G

Q

G

Y

Y

Y

F

C

D

S

L

V

E

G

P

A

M

T

D

N

E

A

A

-
x
P

-

D

V

I

G
x
R

R
|
R

Q

D

I

I

I

V

-

F

-

A

E

L

E

E

H

N

L

L

D

-

T

-

C

-

I

-

E

-

A

-

G

G

L

F

N

H

I

V

E
|
E

G

A

I
x
S

N

H

S

H

E

E

V

V

M

A

M

P

G

G

Q

Q

W

H

E

E

Y

V

Q

D

I
x
F

F
x
K

S
x
F

K

D

N

D

A

A

T

L

E

K

A

T

C

A

D

D

Q

S

I

V

W

I

A

T

S

F

R

K

Y

T

F

T

L

I

E

K

R

T

I

I

T

A

E

E

Q

Q

Y

H

N

G

V

L

K

K

V

A

D

T

W

F

D

M

P

P

K

K

P

P

V

F

Q

F

G

G

D

-

W

M

N

N

G

G

S

S

G

G

M

M

H

H

A

C

N

-

F

-

S

-

N
x
H

K

Q

Q
x
S

L

I

R

W

E

L

S

N

G

G

D

E

K

P

K

S

I

F

F

Y

D

D

-

E

-

N

-

A

-

P

-

Y

-

Q

-

L

-

S

K

E

I

T

C

C

E

M

E

N

F

Y

K

V

-

A

-

G

-

I

L

L

T

K

H

H

K

A

E

K

H

A

I

I

A

V

V

A

Y

I

G

T

D

N

-

P

-

T

-

V

H

N

N

S

E

Y

Q

K

R

R

L

L

T

V

G

P

E

G

H

Y

E

E

T

A

Q

P

D

-

I

V

N

N

S

-

F

I

S

A

Y

W

G

A

V

N

S

S

D

N

R

R

S

S

A

A

S

I

L

I

R
|
R

I

V

P

P

I

A

A

A

T

R

I

G

K

K

N

G

N

T

W

-

K

-

G

-

R
|
R

I

I

E

E

D

F

R

R

R

A

P

P

A

D

S

P

N

S

A

C

N

N

P

P

Y

Y

D

L

V

A

A

F

A

T

I

V

I

M

I

L

K

A

S

A

binding 2-oxoglutaric acid: R87 (≠ D67), V93 (≠ A73), P170 (vs. gap), R173 (≠ G146), R174 (= R147), S190 (≠ I167)
binding adenosine-5'-triphosphate: E136 (= E107), E188 (= E165), F203 (≠ I180), K204 (≠ F181), F205 (≠ S182), H251 (≠ N232), S253 (≠ Q234), R325 (= R294), R335 (= R307)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 17, 19, 33

8ooqB Glutamine synthetase from Methanothermococcus thermolithotrophicus (see paper)
26% identity, 86% coverage: 42:329/335 of query aligns to 55:356/446 of 8ooqB

query
sites
8ooqB

F

F

D

D

G

G

S

S

T

I

K

E

Q

G

A

F

V

V

G

G

-

I

N

N

N

E

S

S

D

D

C

M

L

M

L

L

K

K

P

P

-

D

-

L

-

S

-

T

-

F

V

S

A

V

L

L

Y

P

L

W

D
x
R

P

P

G

S

R

E

I

K

D

S

A
x
V

Y

A

I

R

V

V

I

I

N

C

E

D

V

V

L

Y

N

T

S

T

D

K

E

G

T

K

P

P

H

F

I

E

S

G

N

D

K

P

R

R

A

G

T

C

I

L

-

K

-

R

-

V

-

M

-

E

-

F

-

K

E

E

E

F

N

N

D

G

D

E

F

Y

W

F

F

V

G

G

F

P

E

E

Q

P

E
|
E

Y

F

T

F

L

L

I

L

D

K

L

K

E

D

T

P

N

H

R

N

P

P

I

H

G

K

F

Y

P

-

V

-

N

-

G

-

Y

I

P

P

S

A

P

D

Q

D

G

G

Q

G

Y

Y

Y

F

C

D

S

L

V

E

G

P

A

M

T

D

N

E

A

A

-
x
P

-

D

V

I

G
x
R

R
|
R

Q

D

I

I

I

V

-

F

-

A

E

L

E

E

H

N

L

L

D

-

T

-

C

-

I

-

E

-

A

-

G

G

L

F

N

H

I

V

E

E

G

A

I
x
S

N

H

S

H

E
|
E

V

V

M

A

M

P

G

G

Q

Q

W

H

E
|
E

Y

V

Q

D

I

F

F

K

S

F

K

D

N

D

A

A

T

L

E

K

A

T

C

A

D

D

Q

S

I

V

W

I

A

T

S

F

R

K

Y

T

F

T

L

I

E

K

R

T

I

I

T

A

E

E

Q

Q

Y

H

N

G

V

L

K

K

V

A

D

T

W

F

D

M

P

P

K

K

P

P

V

F

Q

F

G

G

D

-

W

M

N

N

G

G

S

S

G

G

M

M

H

H

A

C

N

-

F

-

S

-

N

H

K

Q

Q

S

L

I

R

W

E

L

S

N

G

G

D

E

K

P

K

S

I

F

F

Y

D

D

-

E

-

N

-

A

-

P

-

Y

-

Q

-

L

-

S

K

E

I

T

C

C

E

M

E

N

F

Y

K

V

-

A

-

G

-

I

L

L

T

K

H

H

K

A

E

K

H

A

I

I

A

V

V

A

Y

I

G

T

D

N

-

P

-

T

-

V

H

N

N

S

E

Y

Q

K

R

R

L

L

T

V

G

P

E

G

H

Y

E

E

T

A

Q

P

D

-

I

V

N

N

S

-

F

I

S

A

Y

W

G

A

V

N

S

S

D

N

R

R

S

S

A

A

S

I

L

I

R

R

I

V

P

P

I

A

A

A

T

R

I

G

K

K

N

G

N

T

W

-

K

-

G

-

R

R

I

I

E

E

D

F

R

R

R

A

P

P

A

D

S

P

N

S

A

C

N

N

P

P

Y

Y

D

L

V

A

A

F

A

T

I

V

I

M

I

L

K

A

S

A

binding 2-oxoglutaric acid: R86 (≠ D67), V92 (≠ A73), P169 (vs. gap), R172 (≠ G146), R173 (= R147), S189 (≠ I167)
binding magnesium ion: E137 (= E109), E192 (= E170), E199 (= E177)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 16, 18, 32

7tenA Glutamine synthetase (see paper)
27% identity, 86% coverage: 42:329/335 of query aligns to 50:351/442 of 7tenA

query
sites
7tenA

F

F

D

D

G

G

S

S

T

I

K

E

Q

G

A

F

V

V

G

R

-

I

N

E

S

S

D

D

C

M

L

Y

L

L

K

F

P

P

-

D

-

L

-

D

-

T

-

W

V

V

A

V

L

F

Y

P

L

W

D

T

P

A

G

E

R

K

I

G

D

K

A

V

Y

A

I

R

V

M

I

I

N

C

E

D

V

I

L

Y

N

N

S

P

D

D

E

M

T

T

P

P

H

F

I

A

S

G

N

D

K

P

R

R

A

A

-

N

-

L

-

K

-

R

T

V

I

L

E

K

E

E

N

M

D

E

F

L

W

G

F

F

-

T

-

E

-

F

-

N

-

L

G
|
G

F

P

E
|
E

Q

P

E
|
E

Y

F

T

F

L

L

I

F

D

K

L

L

E

D

T

E

N

N

R

R

P

R

I

P

G

T

F

L

P

E

V

L

N

N

G

D

Y

-

P

-

S

-

P

-

Q

S

G

G

Q

G

Y

Y

Y

F

C

D

S

L

V

-

G

-

A

A

T

P

N

T

A

D

V

L

G

G

R

E

Q

N

I

C

I

R

E

R

E

D

H

I

L

V

D

L

T

E

C

L

I

E

E

E

A

M

G

G

L

F

N

E

I

I

E
|
E

G

A

I

S

N

H

S

H

E
|
E

V

V

M

A

M

P

G

G

Q

Q

W

H

E
|
E

Y

I

Q
x
D

I
x
F

F
x
K

S
x
Y

K

E

N

D

A

A

T

I

E

T

A

A

C

C

D

D

Q

S

I

I

W

Q

A

T

S

F

R

K

Y

L

F

V

L

V

E

K

R

T

I

I

T

A

E

R

Q

K

Y

H

N

G

V

L

K

H

V

A

D

T

W

F

D

M

P

P

K

K

P

P

V

L

Q

F

G

G

D

-

W

V

N
|
N

G
|
G

S

S

G

G

M

M

H
|
H

A

F

N
|
N

F

M

S
|
S

N

-

K

-

Q

L

L

F

R

N

E

E

S

K

G

G

D

N

K

-

K

A

I

F

F

F

D

D

K

E

I

-

C

S

E

G

E

E

F

L

K

E

L

L

T

S

H

Q

K

T

E

A

H

Y

I

H

A

F

V

L

Y

A

G

G

D

M

H

L

N

K

E

H

Q

A

R

R

L

G

T

Y

G

T

E

A

H

V

E

T

T

N

Q

P

D

T

I

I

N

N

S

S

F

F

S

K

Y
x
R

G

L

V

V

-

P

-

G

-

Y

-
x
E

-

A

-

P

-

C

-

Y

-

I

-

A

-

W

-

S

-

G

S

K

D

N

R
|
R

S

S

A

P

S

L

L

V

R
|
R

I

V

P

P

I

S

A

S

T

-

I

-

K

-

N

R

N

G

W

L

K
x
S

G

T

R
|
R

I

L

E

E

D

L

R
|
R

R

S

P

V

A

D

S

P

N

S

A

A

N

N

P

P

Y

Y

D

L

V

A

A

M

A

A

I

V

I

L

K

K

S

A

binding adenosine-5'-diphosphate: G128 (= G105), E130 (= E107), E182 (= E165), D196 (≠ Q179), F197 (≠ I180), K198 (≠ F181), Y199 (≠ S182), N245 (= N229), S247 (= S231), R319 (= R294), S327 (≠ K305), R329 (= R307)
binding l-methionine-s-sulfoximine phosphate: E130 (= E107), E132 (= E109), E187 (= E170), E194 (= E177), N238 (= N222), G239 (= G223), H243 (= H227), R296 (≠ Y284), E302 (vs. gap), R314 (= R289), R333 (= R311)

7tf9S L. Monocytogenes gs(14)-q-glnr peptide (see paper)
27% identity, 86% coverage: 42:329/335 of query aligns to 51:352/443 of 7tf9S

query
sites
7tf9S

F

F

D

D

G

G

S

S

T

I

K

E

Q

G

A
x
F

V
|
V

G

R

-

I

N

E

S

S

D

D

C

M

L

Y

L

L

K

F

P

P

-

D

-

L

-

D

-

T

-

W

V

V

A

V

L

F

Y

P

L

W

D

T

P

A

G

E

R

K

I

G

D

K

A

V

Y

A

I

R

V

M

I

I

N

C

E

D

V

I

L

Y

N

N

S

P

D

D

E

M

T

T

P

P

H

F

I

A

S

G

N

D

K

P

R

R

A

A

-

N

-

L

-

K

-

R

T

V

I

L

E

K

E

E

N

M

D

E

F

L

W

G

F

F

-

T

-

E

-

F

-

N

-

L

G

G

F

P

E
|
E

Q

P

E
|
E

Y

F

T

F

L

L

I

F

D

K

L

L

E

D

T

E

N

N

R

R

P

R

I

P

G

T

F

L

P

E

V

L

N

N

G

D

Y

-

P

-

S

-

P

-

Q

S

G

G

Q

G

Y
|
Y

Y

F

C

D

S

L

V

-

G

-

A

A

T

P

N

T

A

D

V

L

G

G

R

E

Q

N

I

C

I

R

E

R

E

D

H

I

L

V

D

L

T

E

C

L

I

E

E

E

A

M

G

G

L

F

N

E

I

I

E

E

G

A

I

S

N

H

S

H

E
|
E

V

V

M

A

M

P

G

G

Q

Q

W

H

E
|
E

Y

I

Q

D

I

F

F

K

S

Y

K

E

N

D

A

A

T

I

E

T

A

A

C

C

D

D

Q

S

I

I

W

Q

A

T

S

F

R

K

Y

L

F

V

L

V

E

K

R

T

I

I

T

A

E

R

Q

K

Y

H

N

G

V

L

K

H

V

A

D

T

W

F

D

M

P

P

K

K

P

P

V

L

Q

F

G

G

D

-

W

V

N

N

G
|
G

S

S

G
|
G

M

M

H
|
H

A

F

N

N

F

M

S

S

N

-

K

-

Q

L

L

F

R

N

E

E

S

K

G

G

D

N

K

-

K

A

I

F

F

F

D

D

K

E

I

-

C

S

E

G

E

E

F

L

K

E

L

L

T

S

H

Q

K

T

E

A

H

Y

I

H

A

F

V

L

Y

A

G

G

D

M

H

L

N

K

E

H

Q

A

R

R

L

G

T

Y

G

T

E

A

H

V

E

T

T

N

Q

P

D

T

I

I

N

N

S

S

F

F

S

K

Y
x
R

G

L

V

V

-

P

-

G

-

Y

-
x
E

-

A

-

P

-

C

-

Y

-

I

-

A

-

W

-

S

-

G

S

K

D

N

R

R

S

S

A

P

S

L

L

V

R

R

I

V

P

P

I

S

A

S

T

-

I

-

K

-

N

R

N

G

W

L

K

S

G

T

R

R

I

L

E
|
E

D

L

R

R

R

S

P

V

A

D

S

P

N

S

A

A

N

N

P

P

Y

Y

D

L

V

A

A

M

A

A

I

V

I

L

K

K

S

A

binding glutamine: E133 (= E109), Y155 (= Y135), E188 (= E170), G240 (= G223), G242 (= G225), R297 (≠ Y284), E303 (vs. gap)
binding magnesium ion: E131 (= E107), E133 (= E109), E188 (= E170), E195 (= E177), H244 (= H227), E332 (= E309)
binding : F59 (≠ A50), V60 (= V51)

Sites not aligning to the query:

binding : 418, 422, 426

Query Sequence

>WP_013134073.1 NCBI__GCF_000092245.1:WP_013134073.1
MTKTKLEYIWLDGYKPTQSLRSKTMVVSDFSGKLEDCDIWSFDGSSTKQAVGNNSDCLLK
PVALYLDPGRIDAYIVINEVLNSDETPHISNKRATIEENDDFWFGFEQEYTLIDLETNRP
IGFPVNGYPSPQGQYYCSVGATNAVGRQIIEEHLDTCIEAGLNIEGINSEVMMGQWEYQI
FSKNATEACDQIWASRYFLERITEQYNVKVDWDPKPVQGDWNGSGMHANFSNKQLRESGD
KKIFDKICEEFKLTHKEHIAVYGDHNEQRLTGEHETQDINSFSYGVSDRSASLRIPIATI
KNNWKGRIEDRRPASNANPYDVAAIIIKSVKKALN

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory