SitesBLAST
Comparing WP_013134260.1 NCBI__GCF_000092245.1:WP_013134260.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5temA Structure of 4-hydroxy-tetrahydrodipicolinate reductase from vibrio vulnificus with 2,6 pyridine dicarboxylic and nadh (see paper)
40% identity, 100% coverage: 1:257/257 of query aligns to 1:266/266 of 5temA
- active site: H155 (= H146), K159 (= K150)
- binding nicotinamide-adenine-dinucleotide: G8 (= G8), G11 (= G11), R12 (= R12), M13 (≠ V13), E34 (vs. gap), R35 (vs. gap), F75 (= F65), T76 (≠ S66), S80 (≠ A70), G98 (≠ A90), T100 (= T92), P123 (≠ T115), N124 (= N116), Y125 (≠ M117), F239 (= F230)
- binding pyridine-2,6-dicarboxylic acid: T100 (= T92), P123 (≠ T115), H156 (= H147), K159 (= K150), S164 (= S155), G165 (= G156), T166 (= T157)
4ywjA Crystal structure of 4-hydroxy-tetrahydrodipicolinate reductase (htpa reductase) from pseudomonas aeruginosa
40% identity, 100% coverage: 1:257/257 of query aligns to 1:267/268 of 4ywjA
- active site: H156 (= H146), K160 (= K150)
- binding nicotinamide-adenine-dinucleotide: G11 (= G11), R12 (= R12), M13 (≠ V13), D35 (≠ S35), R36 (≠ N36), F76 (= F65), T77 (≠ S66), V81 (≠ A70), G99 (≠ A90), T101 (= T92), A124 (≠ T115), N125 (= N116), F126 (≠ M117), R237 (= R227), F240 (= F230)
5tejB Structure of 4-hydroxy-tetrahydrodipicolinate reductase from vibrio vulnificus with 2,5 furan dicarboxylic and nadh (see paper)
40% identity, 100% coverage: 1:257/257 of query aligns to 1:266/269 of 5tejB
- active site: H155 (= H146), K159 (= K150)
- binding 2,5 Furan Dicarboxylic Acid: T100 (= T92), H156 (= H147), K159 (= K150), S164 (= S155), G165 (= G156), T166 (= T157)
- binding nicotinamide-adenine-dinucleotide: G8 (= G8), G11 (= G11), R12 (= R12), M13 (≠ V13), E34 (vs. gap), R35 (vs. gap), F75 (= F65), T76 (≠ S66), S80 (≠ A70), G98 (≠ A90), T100 (= T92), P123 (≠ T115), N124 (= N116), Y125 (≠ M117), F239 (= F230)
5tejA Structure of 4-hydroxy-tetrahydrodipicolinate reductase from vibrio vulnificus with 2,5 furan dicarboxylic and nadh (see paper)
40% identity, 100% coverage: 1:257/257 of query aligns to 1:266/269 of 5tejA
- active site: H155 (= H146), K159 (= K150)
- binding nicotinamide-adenine-dinucleotide: G8 (= G8), G11 (= G11), R12 (= R12), M13 (≠ V13), E34 (vs. gap), R35 (vs. gap), F75 (= F65), T76 (≠ S66), S80 (≠ A70), G98 (≠ A90), T100 (= T92), P123 (≠ T115)
3ijpA Crystal structure of dihydrodipicolinate reductase from bartonella henselae at 2.0a resolution (see paper)
41% identity, 98% coverage: 6:257/257 of query aligns to 6:266/266 of 3ijpA
- active site: H155 (= H146), K159 (= K150)
- binding sodium ion: I21 (≠ L21), Q22 (= Q22), R24 (≠ D24), V27 (= V27)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G8), N10 (≠ T10), G11 (= G11), R12 (= R12), M13 (≠ V13), R35 (vs. gap), F75 (= F65), S76 (= S66), Q77 (≠ V67), A80 (= A70), G98 (≠ A90), T100 (= T92), G123 (≠ T115), N124 (= N116), M125 (= M117), F239 (= F230)
3ijpB Crystal structure of dihydrodipicolinate reductase from bartonella henselae at 2.0a resolution (see paper)
41% identity, 98% coverage: 6:257/257 of query aligns to 6:266/267 of 3ijpB
1drvA Escherichia coli dhpr/acnadh complex (see paper)
38% identity, 100% coverage: 2:257/257 of query aligns to 3:267/270 of 1drvA
- active site: H156 (= H146), K160 (= K150)
- binding 3-acetylpyridine adenine dinucleotide: G9 (= G8), G12 (= G11), R13 (= R12), M14 (≠ V13), E35 (vs. gap), F76 (= F65), T77 (≠ S66), R78 (≠ V67), G81 (≠ A70), G99 (≠ A90), A124 (≠ T115), F126 (≠ M117), R237 (= R227)
1druA Escherichia coli dhpr/nadh complex (see paper)
38% identity, 100% coverage: 2:257/257 of query aligns to 3:267/270 of 1druA
- active site: H156 (= H146), K160 (= K150)
- binding nicotinamide-adenine-dinucleotide: G9 (= G8), G12 (= G11), R13 (= R12), M14 (≠ V13), E35 (vs. gap), R36 (vs. gap), F76 (= F65), T77 (≠ S66), R78 (≠ V67), G81 (≠ A70), G99 (≠ A90), T100 (= T91), T101 (= T92), A124 (≠ T115), N125 (= N116), F126 (≠ M117), F240 (= F230)
1arzA Escherichia coli dihydrodipicolinate reductase in complex with nadh and 2,6 pyridine dicarboxylate (see paper)
38% identity, 100% coverage: 2:257/257 of query aligns to 3:267/270 of 1arzA
P04036 4-hydroxy-tetrahydrodipicolinate reductase; HTPA reductase; EC 1.17.1.8 from Escherichia coli (strain K12) (see 3 papers)
38% identity, 100% coverage: 2:257/257 of query aligns to 6:270/273 of P04036
- G12 (= G8) binding NADP(+)
- GRM 15:17 (≠ GRV 11:13) binding NAD(+)
- RM 16:17 (≠ RV 12:13) binding NADP(+)
- E38 (vs. gap) binding NAD(+)
- R39 (vs. gap) binding NADP(+)
- TR 80:81 (≠ SV 66:67) binding NAD(+)
- GTT 102:104 (≠ ATT 90:92) binding NAD(+); binding NADP(+)
- AANF 126:129 (≠ ATNM 114:117) binding NAD(+)
- F129 (≠ M117) binding NADP(+)
- H159 (= H146) mutation H->A,Q: 135 to 200-fold reduction in catalytic activity.
- K163 (= K150) binding NAD(+); mutation K->A,C,Q: 625 to 830-fold reduction in catalytic activity.
- R240 (= R227) binding NADP(+)
- F243 (= F230) binding NAD(+)
1arzB Escherichia coli dihydrodipicolinate reductase in complex with nadh and 2,6 pyridine dicarboxylate (see paper)
38% identity, 100% coverage: 2:257/257 of query aligns to 2:266/269 of 1arzB
- active site: H155 (= H146), K159 (= K150)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G8 (= G8), G10 (≠ T10), G11 (= G11), R12 (= R12), M13 (≠ V13), E34 (vs. gap), F75 (= F65), T76 (≠ S66), R77 (≠ V67), G80 (≠ A70), H84 (≠ E72), G98 (≠ A90), T100 (= T92), A123 (≠ T115), N124 (= N116), F125 (≠ M117), F239 (= F230)
- binding pyridine-2,6-dicarboxylic acid: T100 (= T92), H156 (= H147), K159 (= K150), S164 (= S155), G165 (= G156), T166 (= T157), F239 (= F230)
1drwA Escherichia coli dhpr/nhdh complex (see paper)
38% identity, 100% coverage: 2:257/257 of query aligns to 5:269/272 of 1drwA
- active site: H158 (= H146), K162 (= K150)
- binding nicotinamide purin-6-ol-dinucleotide: G11 (= G8), G14 (= G11), R15 (= R12), M16 (≠ V13), E37 (vs. gap), R38 (vs. gap), F78 (= F65), T79 (≠ S66), R80 (≠ V67), G101 (≠ A90), T102 (= T91), T103 (= T92), A126 (≠ T115), N127 (= N116), F128 (≠ M117), F242 (= F230)
1dihA Three-dimensional structure of e. Coli dihydrodipicolinate reductase (see paper)
38% identity, 100% coverage: 2:257/257 of query aligns to 5:269/272 of 1dihA
- active site: H158 (= H146), K162 (= K150)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (= G8), G14 (= G11), R15 (= R12), M16 (≠ V13), R38 (vs. gap), F78 (= F65), T79 (≠ S66), R80 (≠ V67), G83 (≠ A70), G101 (≠ A90), T103 (= T92), N127 (= N116), F128 (≠ M117), R239 (= R227), F242 (= F230)
Q9X1K8 4-hydroxy-tetrahydrodipicolinate reductase; HTPA reductase; EC 1.17.1.8 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
35% identity, 99% coverage: 2:255/257 of query aligns to 1:212/216 of Q9X1K8
1vm6B Crystal structure of dihydrodipicolinate reductase (tm1520) from thermotoga maritima at 2.27 a resolution
35% identity, 99% coverage: 2:255/257 of query aligns to 6:217/218 of 1vm6B
- active site: H132 (= H146), K136 (= K150)
- binding nicotinamide-adenine-dinucleotide: G12 (= G8), S14 (≠ T10), G15 (= G11), R16 (= R12), M17 (≠ V13), D37 (= D51), V38 (≠ I52), F53 (= F65), S54 (= S66), S55 (≠ V67), E57 (≠ A69), A58 (= A70), G76 (≠ A90), T78 (= T92), Y101 (≠ T115), N102 (= N116), F103 (≠ M117), F192 (= F230)
5z2fA NADPH/pda bound dihydrodipicolinate reductase from paenisporosarcina sp. Tg-14 (see paper)
28% identity, 99% coverage: 1:255/257 of query aligns to 2:265/265 of 5z2fA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: R11 (≠ T10), G12 (= G11), K13 (≠ R12), M14 (≠ V13), D35 (vs. gap), H36 (= H33), K37 (vs. gap), L76 (≠ F65), T77 (≠ S66), G99 (≠ A90), T100 (= T91), T101 (= T92), P126 (≠ T115), N127 (= N116), F128 (≠ M117)
- binding pyridine-2,6-dicarboxylic acid: P126 (≠ T115), H155 (= H146), H156 (= H147), K159 (= K150), S164 (= S155), G165 (= G156), T166 (= T157), A215 (≠ G205)
5z2eA Dipicolinate bound dihydrodipicolinate reductase from paenisporosarcina sp. Tg-14 (see paper)
28% identity, 99% coverage: 1:255/257 of query aligns to 2:265/265 of 5z2eA
5eesA Crystal structure of dapb in complex with NADP+ from corynebacterium glutamicum (see paper)
29% identity, 100% coverage: 2:257/257 of query aligns to 2:247/247 of 5eesA
- active site: H133 (= H146), K137 (= K150)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G8), G11 (= G11), R12 (= R12), V13 (= V13), G34 (= G45), V35 (= V48), F53 (= F65), T54 (≠ S66), G76 (≠ A90), T78 (= T92), P104 (≠ T115), N105 (= N116), F106 (≠ M117), F220 (= F230)
- binding sulfate ion: H134 (= H147), K137 (= K150), K137 (= K150), G143 (= G156), T144 (= T157)
5eerA Crystal structure of dapb from corynebacterium glutamicum (see paper)
29% identity, 100% coverage: 2:257/257 of query aligns to 2:247/247 of 5eerA
1yl5A Crystal structure of mycobacterium tuberculosis dihydrodipicolinate reductase (rv2773c) (crystal form a) (see paper)
30% identity, 95% coverage: 1:244/257 of query aligns to 2:233/247 of 1yl5A
Query Sequence
>WP_013134260.1 NCBI__GCF_000092245.1:WP_013134260.1
MINVGILGSTGRVGSLLIDDLQNDEQVKLSACHVSNKLNKTVPQGTVVTNDINTLLESSD
VVIDFSVPAATELLLTEVIEGKNKKPLVIATTGFSKHQQNLLIEASKLVPILYATNMSLG
VAVLNKLVSLASKTLKDFDIEIVEQHHRNKVDSPSGTALTLAEHAASARDLNLDDVRVSG
RDGIIGARSKDEIAVMSLRGGDIVGRHTVGLYNEGEFIELNHTATSRNTFSKGAIKAAKW
LVNKEAGLYSINDCLGL
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory