SitesBLAST
Comparing WP_013134568.1 NCBI__GCF_000092245.1:WP_013134568.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 19 hits to proteins with known functional sites (download)
P38502 Acetate kinase; Acetokinase; EC 2.7.2.1 from Methanosarcina thermophila (see 5 papers)
53% identity, 99% coverage: 1:395/398 of query aligns to 1:396/408 of P38502
- N7 (= N7) mutation to A: Almost abolishes catalytic activity. Requires increased magnesium levels for activity. Strongly decreases affinity for acetate.; mutation to D: Almost abolishes catalytic activity. Strongly decreases affinity for acetate.
- S10 (= S10) mutation S->A,T: Strongly decreases catalytic activity. Strongly decreases affinity for acetate.
- S12 (= S12) mutation to A: Decreases catalytic activity. Strongly decreases affinity for acetate. Requires increased magnesium levels for enzyme activity.; mutation to T: Decreases catalytic activity. Strongly decreases affinity for acetate.
- K14 (= K14) mutation to A: Strongly decreases enzyme activity.; mutation to R: Reduces enzyme activity.
- R91 (= R90) mutation R->A,L: Decreases catalytic activity. Decreases affinity for acetate.
- V93 (= V92) mutation to A: Decreases affinity for acetate.
- L122 (= L121) mutation to A: Decreases affinity for acetate.
- D148 (= D147) active site, Proton donor/acceptor; mutation D->A,E,N: Abolishes catalytic activity. Decreases affinity for acetate, but not for ATP.
- F179 (= F178) mutation to A: Decreases affinity for acetate.
- N211 (= N209) mutation to A: Slightly reduced enzyme activity.
- P232 (= P230) mutation to A: Decreases affinity for acetate.
- R241 (= R239) mutation R->K,L: Decreases catalytic activity. Strongly reduced affinity for ATP.
- E384 (= E383) mutation to A: Almost abolishes catalytic activity. Strongly decreases affinity for acetate. Requires strongly increased magnesium levels for enzyme activity.
1tuuA Acetate kinase crystallized with atpgs (see paper)
53% identity, 99% coverage: 1:395/398 of query aligns to 1:396/399 of 1tuuA
- active site: N7 (= N7), R91 (= R90), H180 (= H179), R241 (= R239), E384 (= E383)
- binding adenosine-5'-diphosphate: K14 (= K14), G210 (= G208), D283 (= D281), F284 (≠ L282), R285 (= R283), G331 (= G329), I332 (= I330), N335 (= N333)
- binding sulfate ion: R91 (= R90), H180 (= H179), G212 (= G210)
1tuuB Acetate kinase crystallized with atpgs (see paper)
53% identity, 99% coverage: 1:395/398 of query aligns to 1:396/398 of 1tuuB
- active site: N7 (= N7), R91 (= R90), H180 (= H179), R241 (= R239), E384 (= E383)
- binding adenosine monophosphate: D283 (= D281), R285 (= R283), G331 (= G329), I332 (= I330), N335 (= N333), S336 (= S334)
- binding trihydrogen thiodiphosphate: H180 (= H179), G212 (= G210), R241 (= R239)
7fj9A Kpacka (pduw) with amppnp complex structure
44% identity, 98% coverage: 3:394/398 of query aligns to 4:391/395 of 7fj9A
7fj8A Kpacka (pduw) with amp complex structure
44% identity, 98% coverage: 3:394/398 of query aligns to 4:391/395 of 7fj8A
4fwsA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with ctp (see paper)
44% identity, 99% coverage: 2:394/398 of query aligns to 3:389/394 of 4fwsA
- active site: N8 (= N7), R83 (= R90), H172 (= H179), R233 (= R239), E378 (= E383)
- binding cytidine-5'-triphosphate: G202 (= G208), N203 (= N209), G204 (= G210), D275 (= D281), L276 (= L282), R277 (= R283), G323 (= G329), I324 (= I330), N327 (= N333)
- binding 1,2-ethanediol: V21 (≠ T20), C24 (≠ G23), H115 (= H122), N203 (= N209), T232 (= T238), R233 (= R239), K262 (= K268)
4fwrA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with cmp (see paper)
44% identity, 99% coverage: 2:394/398 of query aligns to 3:389/394 of 4fwrA
- active site: N8 (= N7), R83 (= R90), H172 (= H179), R233 (= R239), E378 (= E383)
- binding cytidine-5'-monophosphate: G202 (= G208), N203 (= N209), D275 (= D281), L276 (= L282), R277 (= R283), G323 (= G329), I324 (= I330), N327 (= N333)
4fwqA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gtp (see paper)
44% identity, 99% coverage: 2:394/398 of query aligns to 3:389/394 of 4fwqA
- active site: N8 (= N7), R83 (= R90), H172 (= H179), R233 (= R239), E378 (= E383)
- binding guanosine-5'-triphosphate: H172 (= H179), N203 (= N209), G204 (= G210), D275 (= D281), L276 (= L282), R277 (= R283), E280 (≠ I286), G323 (= G329), I324 (= I330), N327 (= N333)
4fwpA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gdp (see paper)
44% identity, 99% coverage: 2:394/398 of query aligns to 3:389/394 of 4fwpA
- active site: N8 (= N7), R83 (= R90), H172 (= H179), R233 (= R239), E378 (= E383)
- binding 1,2-ethanediol: S11 (= S10), H115 (= H122), K262 (= K268)
- binding guanosine-5'-diphosphate: N203 (= N209), D275 (= D281), L276 (= L282), R277 (= R283), E280 (≠ I286), G323 (= G329), I324 (= I330), N327 (= N333), S328 (= S334)
4fwoA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gmp (see paper)
44% identity, 99% coverage: 2:394/398 of query aligns to 3:389/394 of 4fwoA
- active site: N8 (= N7), R83 (= R90), H172 (= H179), R233 (= R239), E378 (= E383)
- binding guanosine-5'-monophosphate: G202 (= G208), N203 (= N209), D275 (= D281), L276 (= L282), R277 (= R283), E280 (≠ I286), G323 (= G329), I324 (= I330), N327 (= N333)
- binding 1,2-ethanediol: E100 (≠ K107), N104 (≠ Q111)
4fwnA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with adenosine tetraphosphate (ap4) (see paper)
44% identity, 99% coverage: 2:394/398 of query aligns to 3:389/394 of 4fwnA
- active site: N8 (= N7), R83 (= R90), H172 (= H179), R233 (= R239), E378 (= E383)
- binding adenosine-5'-tetraphosphate: H172 (= H179), H200 (= H206), N203 (= N209), G204 (= G210), D275 (= D281), L276 (= L282), R277 (= R283), G323 (= G329), I324 (= I330), N327 (= N333)
4fwmA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with atp (see paper)
44% identity, 99% coverage: 2:394/398 of query aligns to 3:389/394 of 4fwmA