SitesBLAST

O25511 UDP-N-acetylglucosamine 4,6-dehydratase (inverting); Pseudaminic acid biosynthesis protein B; UDP-GlcNAc-inverting 4,6-dehydratase; EC 4.2.1.115 from Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) (see paper)
63% identity, 99% coverage: 1:327/330 of query aligns to 7:333/333 of O25511

query
sites
O25511

M

M

F

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N

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TGSF 19:22 (= TGSF 13:16) binding NADP(+)
SRDELK 43:48 (= SRDELK 37:42) binding NADP(+)
DV 67:68 (= DV 61:62) binding NADP(+)
A87 (= A81) binding NADP(+)
K91 (= K85) binding NADP(+)
LS 129:130 (= LS 123:124) binding NADP(+)
K133 (= K127) mutation K->A,E: Loss of activity.
Y141 (= Y135) binding NADP(+)
K145 (= K139) binding NADP(+)
VVGSR 174:178 (≠ VLGSR 168:172) binding NADP(+)

6bwcC X-ray structure of pen from bacillus thuringiensis (see paper)
41% identity, 86% coverage: 2:284/330 of query aligns to 3:294/327 of 6bwcC

query
sites
6bwcC

F

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binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G11), T14 (= T13), G15 (= G14), T16 (≠ S15), I17 (≠ F16), S37 (= S37), R38 (= R38), S39 (≠ D39), D63 (= D61), I64 (≠ V62), V83 (≠ A81), A84 (= A82), K87 (= K85), T125 (≠ L123), S127 (≠ T125), Y137 (= Y135), K141 (= K139), F167 (≠ Y165), V170 (= V168), S173 (= S171), R174 (= R172)
binding uridine-diphosphate-n-acetylglucosamine: K87 (= K85), H88 (= H86), S127 (≠ T125), N128 (≠ D126), Y137 (= Y135), N169 (= N167), S176 (= S174), V177 (= V175), L180 (≠ Y178), T192 (≠ P191), T194 (= T193), M198 (= M197), R200 (= R199), L234 (≠ I233), E265 (= E255)

3w1vA Crystal structure of capsular polysaccharide synthesizing enzyme cape from staphylococcus aureus in complex with inihibitor (see paper)
43% identity, 94% coverage: 2:311/330 of query aligns to 11:318/347 of 3w1vA

query
sites
3w1vA

F

F

N

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D

D

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S

binding [(2R,3S,4R,5R,6R)-5-acetamido-6-[[[(2R,3S,4R,5R)-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,4-bis(oxidanyl)oxan-2-yl]methylimino-azanylidene-azanium: K93 (= K85), Q94 (≠ H86), N175 (= N167), S179 (= S171), R180 (= R172), S182 (= S174), V183 (= V175), L186 (≠ Y178), T198 (≠ P191), I199 (= I192), T200 (= T193), M204 (= M197), R206 (= R199), V240 (≠ I233), R263 (= R252), E266 (= E255)

4g5hA Crystal structure of capsular polysaccharide synthesizing enzyme cape from staphylococcus aureus in complex with by-product (see paper)
43% identity, 94% coverage: 2:311/330 of query aligns to 11:318/346 of 4g5hA

query
sites
4g5hA

F

F

N

D

D

D

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K

N

I

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L

L

L

I

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T

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G

G

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T
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T

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S
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S

F
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F

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x
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x
V

V

G

D

D

L

L

A

A

K

T

A

A

M

L

A

L

P

E

E

L

L

F

P

E

H

A

D

D

-

N

-

A

-

I

-

E

I

I

V

I

G

G

I

T

R
|
R

P

H

G

G

E
|
E

K

K

L

K

H

A

E

E

I

T

M

L

C

L

P

T

S

R

D

E

S
x
Y

H

A

L

Q

T

C

I

E

E

D

F

M

E

G

D

D

H

Y

F

F

V

R

I

V

G

P

P

A

T

D

I

-

K

-

F

-

H

-

A

-

V

S

R

R

D

D

Y

L

F

N

E

Y

N

S

N

N

L

Y

G

V

E

E

K

T

G

G

T

N

Q

E

-

K

V

I

E

T

Q

Q

G
x
S

F
x
Y

E
|
E

Y
|
Y

N

N

S

S

binding nadp nicotinamide-adenine-dinucleotide phosphate: G20 (= G11), T22 (= T13), G23 (= G14), S24 (= S15), F25 (= F16), S45 (= S37), R46 (= R38), D47 (= D39), K50 (= K42), D69 (= D61), V70 (= V62), A89 (= A81), A90 (= A82), A91 (= A83), K93 (= K85), L131 (= L123), T133 (= T125), K147 (= K139), Y173 (= Y165)
binding [(2R,3R,4R,6R)-3-acetamido-6-methyl-4-oxidanyl-5-oxidanylidene-oxan-2-yl] [[(2R,3S,4R,5R)-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] hydrogen phosphate: Q94 (≠ H86), V95 (= V87), K135 (= K127), N175 (= N167), S182 (= S174), V183 (= V175), L186 (≠ Y178), T198 (≠ P191), T200 (= T193), M204 (= M197), V240 (≠ I233), R263 (= R252), E266 (= E255), Y278 (≠ S267), S313 (≠ G306), Y314 (≠ F307), E315 (= E308), Y316 (= Y309)

Sites not aligning to the query:

binding [(2R,3R,4R,6R)-3-acetamido-6-methyl-4-oxidanyl-5-oxidanylidene-oxan-2-yl] [[(2R,3S,4R,5R)-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] hydrogen phosphate: 320

4j2oC Crystal structure of NADP-bound wbjb from a. Baumannii community strain d1279779 (see paper)
45% identity, 85% coverage: 1:279/330 of query aligns to 1:282/316 of 4j2oC

query
sites
4j2oC

M

M

F

F

N

K

D

D

K

K

N

V

I

L

L

L

I

I

T

T

G
|
G

G

G

T
|
T

G
|
G

S
|
S

F
|
F

G

G

K

N

K

A

F

V

T

L

K

K

M

R

I

F

L

L

E

E

K

T

Y

-

K

D

P

I

N

K

K

E

I

I

I

R

I

I

Y

F

S
|
S

R
|
R

D
|
D

E

E

L

K

K
|
K

Q

Q

Y

D

E

D

M

M

S

R

Q

K

D

K

Y

Y

N

H

N

S

K

A

C

K

M

L

R

K

Y

F

F

Y

I

I

G

G

D
|
D

V
|
V

R

R

D

D

A

Y

N

N

R

S

L

I

K

L

K

N

A

A

M

T

N

R

D

G

V

V

D

D

Y

Y

V

I

I

Y

H

H

A
|
A

L

L

K
|
K

H

Q

V

V

P

P

I

S

A

C

E

E

Y

F

N

H

P

P

M

M

E

E

C

A

I

V

K

K

T
|
T

N

N

I

V

D

L

G

G

A

T

Q

E

N

N

V

V

I

L

D

E

A

A

I

I

D

Q

N

N

R

H

V

V

K

K

K

R

V

V

I

V

A

C

L
|
L

S

S

T

T

D

D

K

K

A

A

A

V

N

Y

P

P

V

I

N

N

L

A

Y

M

G

G

A

I

T

S

K
|
K

L

A

V

M

S

M

D

E

K

K

L

V

F

M

V

V

A

A

A

K

N

S

N

R

L

N

V

L

G

E

K

G

R

L

D

D

I

T

S

V

F

I

A

C

V

G

V

T

R

R

Y
|
Y

G

G

N

N

V

V

L

M

G

A

S

S

R

R

G

G

S

S

V

V

V

I

P

P

Y

L

F

F

Q

V

K

D

L

Q

I

I

S

R

K

Q

G

G

A

-

E

K

S

P

L

L

P

T

I

I

T

T

D

D

E

P

K

N

M

M

T

T

R

R

F

F

M

M

I

M

T

T

L

L

E

E

Q

D

G

A

V

V

N

D

F

L

V

V

L

L

K

Y

N

A

F

F

E

E

R

H

M

G

K

E

G

N

G

G

E

D

I

I

F

F

V

V

P

Q

K

K

I

A

P

P

S

A

M

A

K

T

I

I

V

A

D

V

L

L

A

A

K

E

A

A

M

L

A

K

P

Q

E

L

L

L

-

N

-

V

-

E

-

D

-

H

P

P

H

I

D

S

I

I

V

M

G

G

I

T

R

R

P

H

G

G

E

E

K

K

L

A

H

F

E

E

I

A

M

L

C

L

P

S

S

R

D

E

S

M

H

V

L

H

T

A

I

F

E

D

F

Q

E

G

D

D

H

Y

F

F

V

R

I

V

binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G11), T13 (= T13), G14 (= G14), S15 (= S15), F16 (= F16), S36 (= S37), R37 (= R38), D38 (= D39), K41 (= K42), D60 (= D61), V61 (= V62), A80 (= A81), A81 (= A82), A82 (= A83), K84 (= K85), T99 (= T100), L122 (= L123), K138 (= K139), Y164 (= Y165)

3vvcA Crystal structure of capsular polysaccharide synthesizing enzyme cape , k126e, in apo form (see paper)
43% identity, 87% coverage: 2:287/330 of query aligns to 3:290/318 of 3vvcA

query
sites
3vvcA

F

F

N

D

D

D

K

K

N

I

I

L

L

L

I

I

T

T

G
|
G

G

G

T
|
T

G
|
G

S

S

F
|
F

G

G

K

N

K

A

F

V

T

M

K

K

M

R

I

F

L

L

E

D

K

S

Y

-

K

N

P

I

N

K

K

E

I

I

I

R

I

I

Y

F

S
|
S

R
|
R

D
|
D

E

E

L

K

K
|
K

Q

Q

Y

D

E

D

M

I

S

R

Q

K

D

K

Y

Y

N

N

K

S

C

K

M

L

R

K

Y

F

F

Y

I

I

G

G

D
|
D

V
|
V

R
|
R

D

D

A

S

N

Q

R

S

L

V

K

E

K

T

A

A

M

M

N

R

D

D

V

V

D

D

Y

Y

V

V

I

F

H

H

A
|
A

L

L

K
|
K

H

Q

V

V

P

P

I

S

A

C

E

E

Y

F

N

F

P

P

M

V

E

E

C

A

I

V

K

K

T

T

N

N

I

I

D

I

G

G

A

T

Q

E

N

N

V

V

I

L

D

Q

A

S

A

A

I

I

D

H

N

Q

R

N

V

V

K

K

V

V

I

I

A

C

L

L

S
|
S

T
|
T

D

D

K

E

A

A

N

Y

P

P

V

I

N

N

L

A

Y

M

G

G

A

I

T

S

K
|
K

L

A

V

M

S

M

D

E

K

K

L

V

F

F

V

V

A

A

A

K

N

S

N

R

L

N

V

I

G

R

K

S

R

E

D

Q

I

T

S

L

F

I

A

C

V

G

V

T

R

R

Y
|
Y

G
|
G

N

N

V

V

L

M

G

A

S

S

R

R

G

G

S

S

V

V

V

I

P

P

Y

L

F

F

Q

I

K

D

L

K

I

I

S

-

K

K

G

A

A

G

E

E

S

P

L

L

P

T

I

I

T

T

D

D

E

P

K

D

M

M

T

T

R

R

F

F

M

L

I

M

T

S

L

L

E

E

Q

D

G

A

V

V

N

E

F

L

V

V

L

V

K

H

N

A

F

F

E

K

R

H

M

A

K

E

G

T

G

G

E

D

I

I

F

M

V

V

P

Q

K

K

I

A

P

P

S

S

M

S

K

T

I

V

V

G

D

D

L

L

A

A

K

T

A

A

M

L

A

L

P

E

E

L

L

F

P

E

H

A

D

D

-

N

-

A

-

I

-

E

I

I

V

I

G

G

I

T

R

R

P

H

G

G

E

E

K

K

L

K

H

A

E

E

I

T

M

L

C

L

P

T

S

R

D

E

S

Y

H

A

L

Q

T

C

I

E

E

D

F

M

E

G

D

D

H

Y

F

F

V

R

I

V

G

P

P

A

T

D

I

Y

K

E

F

Y

H

N

A

S

binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G11), T14 (= T13), G15 (= G14), F17 (= F16), S37 (= S37), R38 (= R38), D39 (= D39), K42 (= K42), D61 (= D61), V62 (= V62), R63 (= R63), A81 (= A81), A82 (= A82), A83 (= A83), K85 (= K85), S124 (= S124), T125 (= T125), K139 (= K139), Y165 (= Y165), G166 (= G166)

3vvbA Crystal structure of capsular polysaccharide synthesizing enzyme cape from staphylococcus aureus in apo form (see paper)
43% identity, 73% coverage: 2:243/330 of query aligns to 1:216/270 of 3vvbA

query
sites
3vvbA

F

F

N

D

D

D

K

K

N

I

I

L

L

L

I

I

T

T

G
|
G

G

G

T
|
T

G
|
G

S
|
S

F
|
F

G

G

K

N

K

A

F

V

T

M

K

K

M

R

I

F

L

L

E

D

K

S

Y

-

K

N

P

I

N

K

K

E

I

I

I

R

I

I

Y

F

S
|
S

R
|
R

D
|
D

E

E

L

K

K
|
K

Q

Q

Y

D

E

D

M

I

S

R

Q

K

D

K

Y

Y

N

N

K

S

C

K

M

L

R

K

Y

F

F

Y

I

I

G

G

D
|
D

V
|
V

R

R

D

D

A

S

N

Q

R

S

L

V

K

E

K

T

A

A

M

M

N

R

D

D

V

V

D

D

Y

Y

V

V

I

F

H

H

A

A

A
|
A

L

L

K
|
K

H

Q

V

V

P

P

I

S

A

C

E

E

Y

F

N

F

P

P

M

V

E

E

C

A

I

V

K

K

T

T

N

N

I

I

D

I

G

G

A

T

Q

E

N

N

V

V

I

L

D

Q

A

S

A

A

I

I

D

H

N

Q

R

N

V

V

K

K

V

V

I

I

A

C

L
|
L

S

S

T
|
T

D

D

K

K

A

A

N

Y

P

P

V

I

N

N

L

A

Y

M

G

G

A

I

T

S

K
|
K

L

A

V

M

S

M

D

E

K

K

L

V

F

F

V

V

A

A

A

K

N

S

N

R

L

N

V

I

G

R

K

S

R

E

D

Q

I

T

S

L

F

I

A

C

V

G

V

T

R

R

Y
|
Y

G
|
G

N

N

V

V

L

M

G

P

S

D

R

-

G

-

S

-

V

-

P

-

Y

-

F

-

Q

-

K

-

L

-

I

-

S

-

K

-

G

-

A

-

E

-

S

-

L

-

P

-

I

-

T

-

D

-

E

-

K

-

M

M

T

T

R

R

F

F

M

L

I

M

T

S

L

L

E

E

Q

D

G

A

V

V

N

E

F

L

V

V

L

V

K

H

N

A

F

F

E

K

R

H

M

A

K

E

G

T

G

G

E

D

I

I

F

M

V

V

P

Q

K

K

I

A

P

P

S

S

M

S

K

T

I

V

V

G

D

D

L

L

A

A

K

T

A

A

M

L

A

L

P

A

E

E

binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G11), T12 (= T13), G13 (= G14), S14 (= S15), F15 (= F16), S35 (= S37), R36 (= R38), D37 (= D39), K40 (= K42), D59 (= D61), V60 (= V62), A80 (= A82), A81 (= A83), K83 (= K85), L121 (= L123), T123 (= T125), K137 (= K139), Y163 (= Y165), G164 (= G166)

4tqgA Crystal structure of megavirus udp-glcnac 4,6-dehydratase, 5-epimerase mg534 (see paper)
36% identity, 85% coverage: 2:282/330 of query aligns to 3:266/297 of 4tqgA

query
sites
4tqgA

F

I

N

N

D

D

K

K

N

T

I

I

L

M

I

I

T

F

G
|
G

G

G

T
x
S

G
|
G

S
|
S

F
x
L

G

G

K

N

K

R

F

-

T

-

K

-

M

-

I

L

L

I

E

E

K

T

Y

Y

K

I

P

N

N

N

K

N

I

I

I

V

-

N

Y

Y

S

S

R
|
R

D
|
D

E

E

L

S

K

K

Q

H

Y

W

E

S

M

M

S

E

Q

L

D

K

Y

Y

N

K

N

S

K

D

C

K

M

L

R

K

Y

N

F

I

I

I

G

G

D
|
D

V
x
I

R

R

D

D

A

F

N

E

R

K

L

V

K

Q

A

S

M

I

N

M

D

R

V

I

-

N

-

P

D

D

Y

I

V

I

I

I

H

I

A
|
A

L

L

K
|
K

H

H

V

I

P

D

I

R

A

C

E

E

Y

Y

N

E

P

I

M

N

E

E

C

C

I

L

K

D

T

T

N

N

I

I

D

K

G

G

A

L

Q

Q

N

N

V

V

I

L

D

K

A

V

A

T

I

E

D

I

N

N

R

R

-

S

-

N

-

L

-

S

-

N

V

L

K

K

K

A

V

V

I

C

A

F

L
x
V

S

S

T

T

D

D

K

K

A

A

A

C

N

S

P

P

V

V

N

N

L

S

Y
|
Y

G

G

A

M

T

S

K
|
K

L

A

V

I

S

C

D

E

K

T

L

L

F

V

V

V

A

E

A

K

N

S

N

K

L

Y

V

I

G

-

K

-

R

K

D

D

I

I

S

K

F

Y

A

V

V

C

V

V

R

R

Y
|
Y

G
|
G

N

N

V
|
V

L

L

G

-

S

-

R

-

G

-

S

-

V

-

P

-

Y

-

F

-

Q

-

K

-

L

-

I

-

S

-

K

-

G

-

A

-

E

-

S

N

L

F

P

T

I

L

T

T

D

H

E

T

K

S

M

M

T

T

R

R

F

F

M

I

I

M

T

T

L

L

E

D

Q

D

G

S

V

V

N

K

F

L

V

I

L

E

K

Y

N

A

F

I

E

I

R

N

M

G

K

N

G

S

G

G

E

E

I

I

F

V

V

I

P

P

K

K

I

L

P

N

S

S

M

M

K

Y

I

I

V

K

D

D

L

M

A

I

K

E

A

L

M

F

A

A

P

D

E

K

L

Y

P

P

H

I

D

V

I

I

V

T

G

G

I

L

R

R

P

S

G

G

E

E

K

R

L

M

H

Y

E

E

I

S

M

L

C

I

P

N

S

D

D

T

D

Q

S

S

H

M

L

K

T

T

I

V

E

P

F

K

E

G

D

D

H

Y

F

Y

V

H

I

I

G

L

P

P

T

T

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G12 (= G11), S14 (≠ T13), G15 (= G14), S16 (= S15), L17 (≠ F16), R36 (= R38), D37 (= D39), D59 (= D61), I60 (≠ V62), A81 (= A81), A82 (= A82), A83 (= A83), K85 (= K85), V128 (≠ L123), Y140 (= Y135), K144 (= K139), Y168 (= Y165), G169 (= G166), V171 (= V168)

5bjuA X-ray structure of the pglf dehydratase from campylobacter jejuni in complex with udp and NAD(h) (see paper)
32% identity, 84% coverage: 2:278/330 of query aligns to 22:301/340 of 5bjuA

query
sites
5bjuA

F

L

N

K

D

D

K

K

N

V

I

V

L

L

I

V

T

S

G
|
G

G

A

T

G

G
|
G

S
x
T

F
x
I

G

G

K

S

K

E

F

L

T

C

K

K

M

Q

I

C

L

I

E

-

K

K

Y

F

K

G

P

A

N

K

K

H

I

L

I

I

I

M

Y

V

S
x
D

R
x
H

D

S

E

E

L

Y

K

N

Q

L

Y

Y

E

K

M

I

S

N

Q

D

D

D

Y

L

N

N

-

L

-

Y

N

K

K

E

C

K

M

I

R

T

Y

P

F

I

I

L

G

L

D
x
S

V
x
I

R

L

D

D

A

K

N

Q

R

S

L

L

K

D

K

E

A

V

M

L

N

K

D

T

V

Y

-

K

-

P

D

E

Y

L

V

I

I

L

H

H

A
|
A

L

Y

K
|
K

H

H

V

V

P

P

I

L

A

C

E

E

Y

Q

N

N

P

P

M

H

E

S

C

A

I

V

K

I

T
x
N

N

N

I

I

D

L

G

G

A

T

Q

K

N

I

V

L

I

C

D

D

A

S

A

A

I

K

D

E

N

N

R

K

V

V

K

A

K

K

V

F

I

V

A

M

L
x
I

S

S

T

T

D

D

K
|
K

A

A

A

V

N

R

P

P

V

T

N

N

L

I

Y

M

G

G

A

C

T

T

K
|
K

L

R

V

V

S

C

D

E

K

L

L

Y

F

T

V

L

A

S

A

M

N

S

N

D

L

-

V

-

G

-

K

-

R

E

D

N

I

F

S

E

F

V

A

A

V

C

V

V

R

R

Y
x
F

G
|
G

N
|
N

V
|
V

L

L

G

G

S
|
S

R
x
S

G

G

S
|
S

V
|
V

V

I

P

P

Y

K

F

F

Q

K

K

A

L

Q

I

I

S

A

K

N

G

N

A

-

E

E

S

P

L

L

P
x
T

I
x
L

T
|
T

D

H

E

P

K

D

M
x
I

T

V

R
|
R

F

Y

M

F

I

M

T

L

L

V

E

A

Q

E

G

A

V

V

N

Q

F

L

V

V

L

L

K

Q

N

A

F

G

E

A

R

I

M

A

K

K

G

G

E

E

I

L

F

F

V

V

P

L

K

D

I

M

-

G

P

K

S

P

M

V

K

K

I

I

V

I

D

D

L

L

A

A

K

K

A

K

M

M

A

L

-

L

-

S

-

N

-

R

P

N

E

D

L

L

P

E

H

I

D

K

I

I

V

T

G

G

I

L

R

R

P

K

G

G

E
|
E

K

K

L

L

H

Y

E

E

I

E

M

L

C

L

P

-

S

I

D

D

D

E

S

N

H

D

L

A

T

K

I

T

E

Q

F

Y

E

E

D

S

H

I

F

F

V

V

binding nicotinamide-adenine-dinucleotide: G31 (= G11), G34 (= G14), T35 (≠ S15), I36 (≠ F16), D56 (≠ S37), H57 (≠ R38), S82 (≠ D61), I83 (≠ V62), A104 (= A81), A105 (= A82), A106 (= A83), K108 (= K85), N123 (≠ T100), I146 (≠ L123), K162 (= K139), F184 (≠ Y165), G185 (= G166), N186 (= N167), V187 (= V168), S190 (= S171), S191 (≠ R172)
binding uridine-5'-diphosphate: K150 (= K127), N186 (= N167), S193 (= S174), V194 (= V175), T209 (≠ P191), L210 (≠ I192), T211 (= T193), I215 (≠ M197), R217 (= R199), E279 (= E255)

5bjvA X-ray structure of the pglf udp-n-acetylglucosamine 4,6-dehydratase from campylobacterjejuni, d396n/k397a variant in complex with udp-n- acrtylglucosamine (see paper)
32% identity, 84% coverage: 2:278/330 of query aligns to 22:301/339 of 5bjvA

query
sites
5bjvA

F

L

N

K

D

D

K

K

N

V

I

V

L

L

I

V

T

S

G
|
G

G

A

T

G

G
|
G

S
x
T

F
x
I

G

G

K

S

K

E

F

L

T

C

K

K

M

Q

I

C

L

I

E

-

K

K

Y

F

K

G

P

A

N

K

K

H

I

L

I

I

I

M

Y

V

S
x
D

R
x
H

D

S

E

E

L

Y

K

N

Q

L

Y

Y

E

K

M

I

S

N

Q

D

D

D

Y

L

N

N

-

L

-

Y

N

K

K

E

C

K

M

I

R

T

Y

P

F

I

I

L

G

L

D
x
S

V
x
I

R

L

D

D

A

K

N

Q

R

S

L

L

K

D

K

E

A

V

M

L

N

K

D

T

V

Y

-

K

-

P

D

E

Y

L

V

I

I

L

H

H

A
|
A

L

Y

K
|
K

H
|
H

V

V

P

P

I

L

A

C

E

E

Y

Q

N

N

P

P

M

H

E

S

C

A

I

V

K

I

T
x
N

N

N

I

I

D

L

G

G

A

T

Q

K

N

I

V

L

I

C

D

D

A

S

A

A

I

K

D

E

N

N

R

K

V

V

K

A

K

K

V

F

I

V

A

M

L
x
I

S

S

T
|
T

D

N

K

A

A

A

A

V

N

R

P

P

V

T

N

N

L

I

Y

M

G

G

A

C

T

T

K
|
K

L

R

V

V

S

C

D

E

K

L

L

Y

F

T

V

L

A

S

A

M

N

S

N

D

L

-

V

-

G

-

K

-

R

E

D

N

I

F

S

E

F

V

A

A

V

C

V

V

R

R

Y
x
F

G
|
G

N
|
N

V
|
V

L

L

G

G

S
|
S

R
x
S

G

G

S
|
S

V
|
V

V

I

P

P

Y

K

F

F

Q

K

K

A

L

Q

I

I

S

A

K

N

G

N

A

-

E

E

S

P

L

L

P
x
T

I
x
L

T
|
T

D

H

E

P

K

D

M
x
I

T

V

R
|
R

F

Y

M

F

I

M

T

L

L

V

E

A

Q

E

G

A

V

V

N

Q

F

L

V

V

L

L

K

Q

N

A

F

G

E

A

R

I

M

A

K

K

G

G

E

E

I

L

F

F

V

V

P

L

K

D

I

M

-

G

P

K

S

P

M

V

K

K

I

I

V

I

D

D

L

L

A

A

K

K

A

K

M

M

A

L

-

L

-

S

-

N

-

R

P

N

E

D

L

L

P

E

H

I

D

K

I

I

V

T

G

G

I

L

R
|
R

P

K

G

G

E
|
E

K

K

L

L

H

Y

E

E

I

E

M

L

C

L

P

-

S

I

D

D

D

E

S

N

H

D

L

A

T

K

I

T

E

Q

F

Y

E

E

D

S

H

I

F

F

V

V

binding nicotinamide-adenine-dinucleotide: G31 (= G11), G34 (= G14), T35 (≠ S15), I36 (≠ F16), D56 (≠ S37), H57 (≠ R38), S82 (≠ D61), I83 (≠ V62), A104 (= A81), A105 (= A82), A106 (= A83), K108 (= K85), N123 (≠ T100), I146 (≠ L123), K162 (= K139), F184 (≠ Y165), G185 (= G166), N186 (= N167), V187 (= V168), S190 (= S171), S191 (≠ R172)
binding uridine-diphosphate-n-acetylglucosamine: K108 (= K85), H109 (= H86), T148 (= T125), G185 (= G166), N186 (= N167), S193 (= S174), V194 (= V175), T209 (≠ P191), L210 (≠ I192), T211 (= T193), I215 (≠ M197), R217 (= R199), R276 (= R252), E279 (= E255)

3pvzA Udp-n-acetylglucosamine 4,6-dehydratase from vibrio fischeri
29% identity, 78% coverage: 8:266/330 of query aligns to 34:287/372 of 3pvzA

query
sites
3pvzA

L

L

I

V

T

L

G

G

T

A

G
|
G

S
|
S

F
x
I

G

G

K

Q

K

A

F

V

T

T

K

K

M

E

I

I

L

F

E

-

K

K

Y

R

K

N

P

P

N

Q

K

K

I

L

I

H

I

V

Y

V

S
x
D

R
x
I

D

S

E

E

L

N

K

N

Q

M

Y

V

E

E

M

L

S

V

Q

R

D

D

-

I

-

R

-

S

-

F

-

G

Y

Y

N

I

N

N

K

G

C

D

M

F

R

Q

Y

T

F

F

I

A

G

L

D
|
D

V
x
I

R

G

-

S

-

I

D

E

A

Y

N

D

R

A

L

F

K

I

K

K

A

A

M

D

N

G

D

Q

V

Y

D

D

Y

Y

V

V

I

L

H

N

A
x
L

A
x
S

A
|
A

L

L

K
|
K

H

H

V

V

P

R

I

-

A

S

E

E

Y

K

N

D

P

P

-

F

-

T

-

L

M

M

E

R

C

M

I

I

K

D

T

V

N

N

I

V

D

F

G

N

A

T

Q

D

N

K

V

T

I

I

D

Q

A

Q

A

S

I

I

D

D

N

A

R

G

V

A

K

K

V

Y

I

F

A

C

L
x
V

S

S

T

T

D
|
D

K

K

A

A

N

N

P

P

V

V

N

N

L

M

Y

M

G

G

A

A

T

S

K
|
K

L

R

V

I

S

M

D

E

K

M

L

F

F

L

V

M

A

R

A

K

N

S

N

-

L

-

V

-

G

-

K

-

R

E

D

E

I

I

S

A

F

I

A

S

V

T

V

A

R

R

Y

F

G

A

N

N

V
|
V

L

A

G

F

S
|
S

R

D

G

G

S

S

V

L

P

H

Y

G

F

F

Q

N

K

Q

L

R

I

I

S

Q

K

K

G

N

A

Q

E

P

S

I

L

V

P

A

I

P

T

N

D

D

E

I

K

K

M

-

T

-

R

R

F

Y

M

F

I

V

T

T

L

P

E

Q

Q

E

G

S

V

G

N

E

F

L

V

C

L

L

K

M

N

S

F

C

E

I

R

F

M

G

K

E

G

N

G

R

E

D

I

I

F

F

V

F

P

P

K

K

I

L

P

S

-

E

S

A

M

L

K

H

I

L

V

I

D

S

L

F

A

A

K

D

A

I

M

A

A

V

P

K

E

Y

L

L

P

K

H

Q

D

-

I

-

V

L

G

G

I

Y

R

E

P

P

G

H

E

-

K

-

L

-

H

-

E

-

I

-

M

L

C

C

P

E

S

S

D

E

D

D

binding nicotinamide-adenine-dinucleotide: G40 (= G14), S41 (= S15), I42 (≠ F16), D62 (≠ S37), I63 (≠ R38), D92 (= D61), I93 (≠ V62), L114 (≠ A81), S115 (≠ A82), A116 (= A83), K118 (= K85), V158 (≠ L123), D161 (= D126), K174 (= K139), V198 (= V168), S201 (= S171)

3pvzB Udp-n-acetylglucosamine 4,6-dehydratase from vibrio fischeri
33% identity, 51% coverage: 8:176/330 of query aligns to 32:204/300 of 3pvzB

query
sites
3pvzB

L

L

I

V

T

L

G

G

T

A

G
|
G

S
|
S

F
x
I

G

G

K

Q

K

A

F

V

T

T

K

K

M

E

I

I

L

F

E

-

K

K

Y

R

K

N

P

P

N

Q

K

K

I

L

I

H

I

V

Y

V

S
x
D

R
x
I

D

S

E

E

L

N

K

N

Q

M

Y

V

E

E

M

L

S

V

Q

R

D

D

-

I

-

R

-

S

-

F

-

G

Y

Y

N

I

N

N

K

G

C

D

M

F

R

Q

Y

T

F

F

I

A

G
x
L

D
|
D

V
x
I

R

G

-

S

-

I

D

E

A

Y

N

D

R

A

L

F

K

I

K

K

A

A

M

D

N

G

D

Q

V

Y

D

D

Y

Y

V

V

I

L

H

N

A
x
L

A
x
S

A
|
A

L

L

K
|
K

H

H

V

V

P

R

I

-

A

S

E

E

Y

K

N

D

P

P

-

F

-

T

-

L

M

M

E

R

C

M

I

I

K

D

T

V

N

N

I

V

D

F

G

N

A

T

Q

D

N

K

V

T

I

I

D

Q

A

Q

A

S

I

I

D

D

N

A

R

G

V

A

K

K

V

Y

I

F

A

C

L

V

S

S

T

T

D
|
D

K

K

A

A

N

N

P

P

V

V

N

N

L

M

Y

M

G

G

A

A

T

S

K
|
K

L

R

V

I

S

M

D

E

K

M

L

F

F

L

V

M

A

R

A

K

N

S

N

-

L

-

V

-

G

-

K

-

R

E

D

E

I

I

S

A

F

I

A

S

V

T

V

A

R

R

Y

F

G

A

N
|
N

V
|
V

L

A

G

F

S
|
S

R

D

G

G

S

S

V

R

V

I

binding nicotinamide-adenine-dinucleotide: G38 (= G14), S39 (= S15), I40 (≠ F16), D60 (≠ S37), I61 (≠ R38), L89 (≠ G60), D90 (= D61), I91 (≠ V62), L112 (≠ A81), S113 (≠ A82), A114 (= A83), K116 (= K85), D159 (= D126), K172 (= K139), N195 (= N167), V196 (= V168), S199 (= S171)

6dntA Udp-n-acetylglucosamine 4-epimerase from methanobrevibacter ruminantium m1 in complex with udp-n-acetylmuramic acid (see paper)
26% identity, 89% coverage: 4:298/330 of query aligns to 3:301/310 of 6dntA

query
sites
6dntA

D

D

K

K

N

N

I

V

L

V

I

V

T

T

G
|
G

G

G

T

L

G
|
G

S
x
F

F
x
I

G

G

K

S

K

H

F

I

T

V

K

D

M

A

I

L

L

I

E

D

K

D

Y

-

K

-

P

-

N

N

K

K

I

V

I

T

I

I

Y

I

S
x
D

R
x
N

D
x
L

E
x
S

L
x
S

K
x
G

Q

K

Y

M

E

E

M

N

S

L

Q

N

D

N

Y

P

N

N

N

H

K

E

C

N

M

L

R

T

Y

I

F

I

I

K

G

E

D
|
D

V
x
L

R

M

D

D

A

A

N

D

R

-

L

L

K

E

K

K

A

I

M

L

N

K

D

D

V

K

D

D

Y

Y

V

V

I

F

H

H

A
x
L

A
|
A

L

L

K
x
A

H

S

V

V

P

P

I

G

A

S

E

V

Y

A

N

E

P

P

M

L

E

R

C

Y

I

N

K

Q

T

N

N

N

I

I

D

D

G

A

A

S

Q

L

N

K

V

L

I

F

D

I

A

A

A

C

I

K

D

N

N

N

R

N

V

I

K

K

V

V

I

I

A

F

L
x
S

S
|
S

T
x
S

D
x
S

K
x
A

A

V

A

Y

N

G

-

E

-

N

-

P

-

N

-

M

-

P

-

L

-

K

-

E

-

S

-

E

-

N

-

F

-

L

P

P

V

C

N

S

L

P

Y
|
Y

G

A

A

A

T

Q

K
|
K

L

A

V

S

S

C

D

E

K

L

L

Y

F

L

V

K

A

S

A

F

N

H

N

E

L

S

V

Y

G

G

K

-

R

-

D

-

I

L

S

D

F

Y

A

V

V

A

V

L

R

R

Y
|
Y

G
x
F

N
|
N

V
|
V

L

F

G

G

S

P

R

R

-

Q

-

D

-

E

-

N

-

S

-

P

-

Y

G

A

S
x
A

V
|
V

V

I

P

P

Y
x
K

F

F

Q

I

K

S

L

A

I

I

S

L

K

N

G

G

A

E

E

S

S

P

L
x
V

P
x
I

I
x
Y

T

G

D

D

E

G

K
x
E

M
x
Q

T

S

R
|
R

F

D

M

F

I

I

T

Y

L

V

E

K

Q

E

G

I

V

A

N

K

F

A

V

N

L

I

K

L

N

S

F

A

E

E

R

S

M

D

K

Y

G

N

G

G

E

V

I

I

F

N

V

V

P

A

K

L

I

G

P

K

S

S

M

M

K

T

I
|
I

V

N

D

R

L

L

A

F

K

E

A

I

M

I

A

S

P

D

E

V

L

L

P

E

H

S

D

D

I

I

-

D

-

V

-

K

-

Y

V

L

G

D

I

E

R
|
R

P

P

G

G

E
x
D

K

I

L

K

H
|
H

E

S

I

L

M

-

C

-

P

-

S

A

D

D

D

I

S

S

H

N

L

L

T

D

I

-

E

-

F

-

E

-

D

-

H

-

F

-

V

-

I

-

G

-

P

-

T

K

I

I

K

S

F

F

H

K

A

P

V

D

R

E

D

D

Y

K

F

F

E

E

N

E

N

Q

L

L

G

R

E

E

active site: S120 (≠ T125), S121 (≠ D126), A122 (≠ K127), Y144 (= Y135), K148 (= K139), A187 (≠ S174)
binding (2R)-2-{[(2R,3R,4R,5S,6R)-3-(acetylamino)-2-{[(S)-{[(R)-{[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methoxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]oxy}-5-hydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-4-yl]oxy}propanoic acid: A80 (≠ K85), S120 (≠ T125), S121 (≠ D126), Y144 (= Y135), F172 (≠ G166), N173 (= N167), A187 (≠ S174), V188 (= V175), K191 (≠ Y178), V203 (≠ L190), I204 (≠ P191), Y205 (≠ I192), Q210 (≠ M197), R212 (= R199), I246 (= I233), R269 (= R252), D272 (≠ E255)
binding nicotinamide-adenine-dinucleotide: G10 (= G11), G13 (= G14), F14 (≠ S15), I15 (≠ F16), D33 (≠ S37), N34 (≠ R38), L35 (≠ D39), S36 (≠ E40), S37 (≠ L41), G38 (≠ K42), D57 (= D61), L58 (≠ V62), L76 (≠ A81), A77 (= A82), A78 (= A83), A80 (≠ K85), S118 (≠ L123), S119 (= S124), Y144 (= Y135), K148 (= K139), Y171 (= Y165), V174 (= V168)
binding zinc ion: E209 (≠ K196), H275 (= H258)

1r66A Crystal structure of desiv (dtdp-glucose 4,6-dehydratase) from streptomyces venezuelae with NAD and tyd bound (see paper)
27% identity, 38% coverage: 7:133/330 of query aligns to 3:135/322 of 1r66A

query
sites
1r66A

I

L

L

L

I

V

T

T

G

G

T

A

G
|
G

S
x
F

F
x
I

G

G

K

S

K

H

F

F

T

V

K

R

M

Q

I

L

L

L

E

A

K

G

Y

A

K

Y

P

P

N

D

-

V

-

P

-

A

-

D

K

E

I

V

I

I

I

V

-

L

-
x
D

-
x
S

-
x
L

-
x
T

Y

Y

S

A

R
x
G

D

N

E

R

L

A

K

N

Q

L

Y

A

E

P

M

V

S

D

Q

A

D

D

Y

P

N

R

N

-

K

-

C

-

M

L

R

R

Y

F

F

V

I

H

G

G

D
|
D

V
x
I

R

R

D

D

A

A

N

G

R

L

L

L

K

A

K

R

A

E

M

L

N

R

D

G

V

V

D

D

Y

A

V

I

I

V

H

H

A
x
F

A
|
A

L

E

K
x
S

H
|
H

V

V

P

D

I

R

A

S

E

I

Y

A

N

G

P

A

M

S

E

V

C

F

I

T

K

E

T
|
T

N

N

I

V

D

Q

G

G

A

T

Q

Q

N

T

V

L

I

L

D

Q

A

C

A

A

I

V

D

D

N

A

R

G

V

V

K

G

K

R

V

V

I

V

A

H

L
x
V

S
|
S

T
|
T

D
|
D

K
x
E

A

V

A

Y

N

G

P

S

V

I

N

D

active site: T127 (= T125), D128 (= D126), E129 (≠ K127)
binding nicotinamide-adenine-dinucleotide: G10 (= G14), F11 (≠ S15), I12 (≠ F16), D37 (vs. gap), S38 (vs. gap), L39 (vs. gap), T40 (vs. gap), G43 (≠ R38), D63 (= D61), I64 (≠ V62), F83 (≠ A81), A84 (= A82), A85 (= A83), S87 (≠ K85), T102 (= T100), V125 (≠ L123), S126 (= S124)
binding thymidine-5'-diphosphate: H88 (= H86), E129 (≠ K127)

Sites not aligning to the query:

active site: 151, 155
binding nicotinamide-adenine-dinucleotide: 151, 155, 181
binding thymidine-5'-diphosphate: 180, 190, 191, 206, 208, 215, 250, 274, 277

3ruhA Alternative analogs as viable substrates of udp-hexose 4-epimerases
26% identity, 60% coverage: 1:199/330 of query aligns to 13:234/336 of 3ruhA

query
sites
3ruhA

M

I

F

F

N

S

D

P

K

K

N

T

I

W

L

L

I

I

T

T

G
|
G

G

V

T

A

G
|
G

S
x
F

F
x
I

G

G

K

-

F

-

T

S

K

N

M

L

I

L

L

E

E

K

K

L

Y

L

K

K

P

L

N

N

K

Q

I

V

I

I

-

G

-

L

-
x
D

-
x
N

-

F

-
x
S

-
x
T

-
x
G

-

H

-

Q

Y

Y

S

N

R

L

D

D

E

E

L

V

K

K

Q

T

Y

L

E

V

M

S

S

T

Q

E

D

Q

Y

W

N

S

N

R

K

F

C

C

M

-

R

-

Y

F

F

I

I

E

G

G

D
|
D

V
x
I

R

R

D

D

A

L

N

T

R

T

L

C

K

E

K

Q

A

V

M

M

N

K

D

G

V

V

D

D

Y

H

V

V

I

L

H

H

A
x
Q

A
|
A

L

L

K
x
G

H
x
S

V

V

P

P

I

R

A

S

E

I

Y

V

N

D

P

P

M

I

E

T

C

T

I

N

K

A

T
|
T

N

N

I

I

D

T

G

G

A

F

Q

L

N

N

V

I

I

L

D

H

A

A

I

K

D

N

N

A

R

Q

V

V

K

Q

K

S

V

F

I

T

A

Y

L
x
A

S
x
A

T
x
S

D
x
S

K
x
S

A

T

-

Y

-

G

-

D

-

H

-

P

-

A

-

L

-

P

-

K

-

V

-

E

-

N

-

I

A

G

N

N

P

P

V

L

N

S

L

P

Y
|
Y

G

A

A

V

T

T

K
|
K

L

Y

V

V

S

N

D

E

K

I

L

Y

F

A

V

Q

A

V

A

Y

N

A

N

R

L

T

V

Y

G

G

K

F

R

K

D

T

I

I

S

G

F

-

A

-

V

-

V

L

R

R

Y
|
Y

G

F

N
|
N

V
|
V

L

F

G

G

S

R

R

R

-

Q

-

D

-

P

-
x
N

-

G

-

A

-

Y

G

A

S
x
A

V
|
V

V

I

P

P

Y
x
K

F
x
W

Q

T

K

A

L

A

I

M

S

L

K

K

G

G

A

D

E

D

S

V

L
x
Y

P
x
I

I
x
N

T

G

D

D

E

G

K

E

M

T

T

S

R
|
R

active site: S142 (≠ T125), S143 (≠ D126), S144 (≠ K127), Y166 (= Y135), K170 (= K139), N204 (vs. gap)
binding nicotinamide-adenine-dinucleotide: G23 (= G11), G26 (= G14), F27 (≠ S15), I28 (≠ F16), D47 (vs. gap), N48 (vs. gap), S50 (vs. gap), T51 (vs. gap), G52 (vs. gap), D78 (= D61), I79 (≠ V62), Q98 (≠ A81), A99 (= A82), A100 (= A83), T117 (= T100), A140 (≠ L123), A141 (≠ S124), S142 (≠ T125), Y166 (= Y135), K170 (= K139), Y193 (= Y165), V196 (= V168)
binding [[(2R,3S,4R,5R)-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl][(2R,3R,4R,5R,6R)-6-(hydroxymethyl)-4,5-bis(oxidanyl)-3-(2-oxidanylidenepropyl)oxan-2-yl] hydrogen phosphate: G102 (≠ K85), S103 (≠ H86), S142 (≠ T125), S143 (≠ D126), S144 (≠ K127), Y166 (= Y135), Y193 (= Y165), N195 (= N167), A209 (≠ S174), V210 (= V175), K213 (≠ Y178), W214 (≠ F179), Y225 (≠ L190), I226 (≠ P191), N227 (≠ I192), R234 (= R199)

Sites not aligning to the query:

binding [[(2R,3S,4R,5R)-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl][(2R,3R,4R,5R,6R)-6-(hydroxymethyl)-4,5-bis(oxidanyl)-3-(2-oxidanylidenepropyl)oxan-2-yl] hydrogen phosphate: 271, 294, 297, 298, 301

Query Sequence

>WP_013136746.1 NCBI__GCF_000092245.1:WP_013136746.1
MFNDKNILITGGTGSFGKKFTKMILEKYKPNKIIIYSRDELKQYEMSQDYNNKCMRYFIG
DVRDANRLKKAMNDVDYVIHAAALKHVPIAEYNPMECIKTNIDGAQNVIDAAIDNRVKKV
IALSTDKAANPVNLYGATKLVSDKLFVAANNLVGKRDISFAVVRYGNVLGSRGSVVPYFQ
KLISKGAESLPITDEKMTRFMITLEQGVNFVLKNFERMKGGEIFVPKIPSMKIVDLAKAM
APELPHDIVGIRPGEKLHEIMCPSDDSHLTIEFEDHFVIGPTIKFHAVRDYFENNLGEKG
TQVEQGFEYNSTKNSEWLGEKEFLSLVKKI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory