SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013136878.1 NCBI__GCF_000092245.1:WP_013136878.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
56% identity, 98% coverage: 1:304/309 of query aligns to 1:305/310 of P9WP55

query
sites
P9WP55

M

M

K

S

Y

I

A

A

N

E

D

D

V

I

T

T

E

Q

L

L

I

I

G

G

N

R

T

T

P

P

L

L

V

V

K

R

L

L

K

R

A

V

S

T

T

D

N

G

G

A

T

V

T

A

-

D

V

I

L

V

G

A

K

K

C

L

E

E

F

F

M

F

N

N

P

P

T

A

H

N

S

S

V

V

K
|
K

D

D

R

R

I

I

G

G

F

V

N

A

M

M

I

L

N

Q

E

A

A

A

I

E

K

Q

S

A

G

G

K

L

I

I

T

K

E

P

G

D

T

T

T

I

V

I

I

L

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

S

M

V

V

C

C

A

A

K

R

G

G

I

Y

K

R

L

C

I

V

L

L

T

T

M

M

P

P

S

E

S

T

M

M

S

S

I

L

E

E

R

R

K

M

L

L

K

R

A

A

L

Y

G

G

A

A

Q

E

I

L

V

I

L

L

T

T

E

P

P

G

E

A

K

D

G

G

M

M

K

S

G

G

A

A

V

I

E

A

K

K

A

A

N

E

E

E

L

L

S

A

E

K

S

T

L

D

D

Q

N

R

S

Y

I

F

V

V

L

P

Q

Q

F

F

A

E

N

N

E

P

A

A

N

N

P

P

D

A

I

I

H

H

R

R

K

V

T

T

A

A

L

E

E

E

I

V

L

W

R

R

D

D

T

T

D

D

D

G

K

K

V

V

D

D

V

I

L

V

V

V

I

A

A

G

I

V

G
|
G

T
|
T

G
|
G

S
x
T

I

I

T

T

G

G

I

V

S

A

E

Q

V

V

V

I

K

K

A

E

K

R

N

K

P

P

N

S

V

A

K

R

V

F

I

V

A

A

V

V

E

E

P

P

T

A

D

A

S

S

P

P

V

V

L

L

S

S

G

G

N

Q

P

K

G

G

P

P

H

H

K

P

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

V

P

P

A

P

V

V

L

L

N

D

T

Q

K

D

I

L

Y

V

D

D

E

E

I

I

T

T

V

V

S

G

N

N

D

E

D

D

A

A

M

L

E

N

T

V

A

A

R

R

N

R

L

L

A

A

K

R

D

E

E

E

G

G

L

L

V

V

G

G

I

I

S
|
S

A

S

G

G

A

A

N

A

V

T

F

V

A

A

S

A

T

L

A

Q

L

V

A

A

Q

R

R

R

E

P

E

E

Y

N

K

A

G

G

K

K

T

L

I

I

V

V

T

V

I

V

L

L

C

P

D

D

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

P

L

L

Y

F

K44 (= K43) modified: N6-(pyridoxal phosphate)lysine
N74 (= N73) binding pyridoxal 5'-phosphate
GTGGT 178:182 (≠ GTGGS 177:181) binding pyridoxal 5'-phosphate
S266 (= S265) binding pyridoxal 5'-phosphate

2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
56% identity, 98% coverage: 1:304/309 of query aligns to 1:305/306 of 2q3dA

query
sites
2q3dA

M

M

K

S

Y

I

A

A

N

E

D

D

V

I

T

T

E

Q

L

L

I

I

G

G

N

R

T

T

P

P

L

L

V

V

K

R

L

L

K

R

A

V

S

T

T

D

N

G

G

A

T

V

T

A

-

D

V

I

L

V

G

A

K

K

C

L

E

E

F

F

M

F

N

N

P

P

T

A

H

N

S

S

V

V

K
|
K

D

D

R

R

I

I

G

G

F

V

N

A

M

M

I

L

N

Q

E

A

A

A

I

E

K

Q

S

A

G

G

K

L

I

I

T

K

E

P

G

D

T

T

T

I

V

I

I

L

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

S

M

V

V

C

C

A

A

K

R

G

G

I

Y

K

R

L

C

I

V

L

L

T

T

M

M

P

P

S

E

S

T

M

M

S

S

I

L

E

E

R

R

K

M

L

L

K

R

A

A

L

Y

G

G

A

A

Q

E

I

L

V

I

L

L

T

T

E

P

P

G

E

A

K

D

G

G

M

M

K

S

G

G

A

A

V

I

E

A

K

K

A

A

N

E

E

E

L

L

S

A

E

K

S

T

L

D

D

Q

N

R

S

Y

I

F

V

V

L

P

Q

Q

Q
|
Q

F

F

A

E

N

N

E

P

A

A

N

N

P

P

D

A

I

I

H

H

R

R

K

V

T

T

A

A

L

E

E

E

I

V

L

W

R

R

D

D

T

T

D

D

D

G

K

K

V

V

D

D

V

I

L

V

V

V

I

A

A

G

I
x
V

G
|
G

T
|
T

G
|
G

G

G

S
x
T

I

I

T

T

G

G

I

V

S

A

E

Q

V

V

V

I

K

K

A

E

K

R

N

K

P

P

N

S

V

A

K

R

V

F

I

V

A

A

V

V

E

E

P

P

T

A

D

A

S

S

P

P

V

V

L

L

S

S

G

G

N

Q

P

K

G

G

P

P

H

H

K

P

I

I

Q

Q

G
|
G

I
|
I

G

G

A

A

G

G

F

F

V

V

P

P

A

P

V

V

L

L

N

D

T

Q

K

D

I

L

Y

V

D

D

E

E

I

I

T

T

V

V

S

G

N

N

D

E

D

D

A

A

M

L

E

N

T

V

A

A

R

R

N

R

L

L

A

A

K

R

D

E

E

E

G

G

L

L

V

V

G

G

I

I

S
|
S

A

S

G

G

A

A

N

A

V

T

F

V

A

A

S

A

T

L

A

Q

L

V

A

A

Q

R

R

R

E

P

E

E

Y

N

K

A

G

G

K

K

T

L

I

I

V

V

T

V

I

V

L

L

C
x
P

D
|
D

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

P

L

L

Y

F

active site: K44 (= K43), S266 (= S265), P293 (≠ C292)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K43), T71 (= T70), S72 (= S71), N74 (= N73), T75 (= T74), Q144 (= Q143), V177 (≠ I176), G178 (= G177), T179 (= T178), G180 (= G179), T182 (≠ S181), G222 (= G221), I223 (= I222), S266 (= S265), P293 (≠ C292), D294 (= D293)

4lmaA Crystal structure analysis of o-acetylserine sulfhydrylase cysk1 from microcystis aeruginosa 7806 (see paper)
57% identity, 100% coverage: 1:309/309 of query aligns to 1:313/318 of 4lmaA

query
sites
4lmaA

M

M

K

R

Y

I

A

A

N

H

D

D

V

V

T

T

E

E

L

L

I

V

G

G

N

R

T

T

P

P

L

L

V

V

K

Q

L

L

K

N

K

R

-

I

-

P

-

Q

A

A

S

E

T

G

N

C

G

L

T

G

T

R

V

I

L

V

G

M

K

K

C

L

E

E

F

G

M

M

N

N

P

P

T

A

H

A

S

S

V

V

K
|
K

D

D

R

R

I

I

G

G

F

T

N

H

M

M

I

I

N

N

E

S

A

A

I

E

K

K

S

A

G

G

K

L

I

I

T

N

E

P

G

E

T

T

V

V

-

L

I

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

S

M

V

T

C

A

A

A

K

K

G

G

I

Y

K

R

L

L

I

I

L

L

T

T

M

M

P

P

S

E

S

T

M

M

S

S

I

L

E

E

R

R

K

A

L

M

L

L

K

K

A

A

L

Y

G

G

A

A

Q

T

I

L

V

E

L

L

T

T

E

P

P

G

E

S

K

Q

G

G

M

M

K

K

G

G

A

A

V

I

E

L

K

R

A

A

N

Q

E

Q

L

I

S

V

E

D

S

S

L

I

D

P

N

G

S

A

I

Y

V

M

L

L

Q

Q

F

F

A

R

N

N

E

P

A

S

N

N

P

P

D

E

I

I

H

H

R

R

K

L

T

T

A

A

L

E

E

E

I

I

L

W

R

Q

D

D

T

T

D

E

D

G

K

Q

V

V

D

D

V

F

L

I

V

V

I

A

A

G

I

V

G
|
G

T
|
T

G
|
G

G

G

S
x
T

I

I

T

T

G

G

I

V

S

A

E

E

V

V

V

I

K

K

A

S

K

R

N

K

P

P

N

S

V

F

K

Q

V

V

I

V

A

A

V

V

E

E

P

P

T

F

D

N

S

S

P

P

V

V

L

I

S

S

G

G

N

N

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

A

E

V

V

L

L

N

R

T

T

K

D

I

L

Y

I

D

D

E

E

I

V

I

I

T

T

V

V

S

S

N

D

D

E

A

A

M

F

E

Q

T

F

A

G

R

R

N

R

L

L

A

A

K

K

D

E

E

E

G

G

L

L

V

S

G

G

I

I

S
|
S

A

S

G

G

A

A

N

N

V

L

F

C

A

A

S

A

T

I

A

Q

L

L

A

A

Q

Q

R

R

E

P

E

E

Y

N

K

E

G

G

K

K

T

L

I

I

V

V

T

V

I

I

L

Q

C
x
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

L

L

M

Y

F

Q

Q

F

N

D

I

D

E

E

E

active site: K46 (= K43), S269 (= S265)
binding pyridoxal-5'-phosphate: K46 (= K43), N77 (= N73), G181 (= G177), T182 (= T178), G183 (= G179), T185 (≠ S181), G225 (= G221), S269 (= S265), P296 (≠ C292)

3zeiA Structure of the mycobacterium tuberculosis o-acetylserine sulfhydrylase (oass) cysk1 in complex with a small molecule inhibitor (see paper)
56% identity, 97% coverage: 1:299/309 of query aligns to 1:300/300 of 3zeiA

query
sites
3zeiA

M

M

K

S

Y

I

A

A

N

E

D

D

V

I

T

T

E

Q

L

L

I

I

G

G

N

R

T

T

P

P

L

L

V

V

K

R

L

L

K

R

A

V

S

T

T

D

N

G

G

A

T

V

T

A

-

D

V

I

L

V

G

A

K

K

C

L

E

E

F

F

M

F

N

N

P

P

T

A

H

N

S

S

V

V

K
|
K

D

D

R

R

I

I

G

G

F

V

N

A

M

M

I

L

N

Q

E

A

A

A

I

E

K

Q

S

A

G

G

K

L

I

I

T

K

E

P

G

D

T

T

T

I

V

I

I

L

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

S

M

V

V

C

C

A

A

K

R

G

G

I

Y

K

R

L

C

I

V

L

L

T

T

M

M

P

P

S

E

S

T

M

M

S

S

I

L

E

E

R

R

K

M

L

L

K

R

A

A

L

Y

G

G

A

A

Q

E

I

L

V

I

L

L

T

T

E

P

P

G

E

A

K

D

G

G

M

M

K

S

G

G

A

A

V
x
I

E

A

K

K

A

A

N

E

E

E

L

L

S

A

E

K

S

T

L

D

D

Q

N

R

S

Y

I

F

V

V

L

P

Q

Q

Q
|
Q

F
|
F

A

E

N

N

E

P

A

A

N

N

P

P

D

A

I

I

H

H

R

R

K

V

T

T

A

A

L

E

E

E

I

V

L

W

R

R

D

D

T

T

D

D

D

G

K

K

V

V

D

D

V

I

L

V

V

V

I

A

A

G

I
x
V

G
|
G

T
|
T

G
|
G

G

G

S
x
T

I

I

T

T

G

G

I

V

S

A

E

Q

V

V

V

I

K

K

A

E

K

R

N

K

P

P

N

S

V

A

K

R

V

F

I

V

A

A

V

V

E

E

P

P

T

A

D

A

S

S

P

P

V

V

L

L

S

S

G

G

N

Q

P
x
K

G

G

P

P

H

H

K

P

I

I

Q

Q

G
|
G

I

I

G

G

A
|
A

G

G

F
|
F

V

V

P

P

A

P

V

V

L

L

N

D

T

Q

K

D

I

L

Y

V

D

D

E

E

I

I

T

T

V

V

S

G

N

N

D

E

D

D

A

A

M

L

E

N

T

V

A

A

R

R

N

R

L

L

A

A

K

R

D

E

E

E

G

G

L

L

V

V

G

G

I

I

S
|
S

A

S

G

G

A

A

N

A

V

T

F

V

A

A

S

A

T

L

A

Q

L

V

A

A

Q

R

R

R

E

P

E

E

Y

N

K

A

G

G

K

K

T

L

I

I

V

V

T

V

I

V

L

L

C
x
P

D
|
D

T

F

G

G

E

E

R

R

Y

Y

L

L

active site: K44 (= K43), S266 (= S265), P293 (≠ C292)
binding 3-[(Z)-[(5Z)-5-[[2-(2-hydroxy-2-oxoethyloxy)phenyl]methylidene]-3-methyl-4-oxidanylidene-1,3-thiazolidin-2-ylidene]amino]benzoic acid: T71 (= T70), S72 (= S71), I126 (≠ V125), Q144 (= Q143), F145 (= F144), K215 (≠ P214), G222 (= G221), A225 (= A224), F227 (= F226)
binding pyridoxal-5'-phosphate: K44 (= K43), N74 (= N73), V177 (≠ I176), G178 (= G177), T179 (= T178), G180 (= G179), T182 (≠ S181), G222 (= G221), S266 (= S265), P293 (≠ C292), D294 (= D293)

2q3cA 2.1 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) holoenzyme from mycobacterium tuberculosis in complex with the inhibitory peptide dfsi (see paper)
56% identity, 97% coverage: 1:299/309 of query aligns to 1:300/300 of 2q3cA

query
sites
2q3cA

M

M

K

S

Y

I

A

A

N

E

D

D

V

I

T

T

E

Q

L

L

I

I

G

G

N

R

T

T

P

P

L

L

V

V

K

R

L

L

K

R

A

V

S

T

T

D

N

G

G

A

T

V

T

A

-

D

V

I

L

V

G

A

K

K

C

L

E

E

F

F

M

F

N

N

P

P

T

A

H

N

S

S

V

V

K
|
K

D

D

R

R

I

I

G

G

F

V

N

A

M

M

I

L

N

Q

E

A

A

A

I

E

K

Q

S

A

G

G

K

L

I

I

T

K

E

P

G

D

T

T

T

I

V

I

I

L

E

E

P

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

A

A

L

L

A

A

S

M

V

V

C

C

A

A

K

R

G

G

I

Y

K

R

L

C

I

V

L

L

T

T

M

M

P

P

S

E

S

T

M

M

S

S

I

L

E

E

R

R

K

M

L

L

K

R

A

A

L

Y

G

G

A

A

Q

E

I

L

V

I

L

L

T

T

E

P

P

G

E

A

K

D

G

G

M
|
M

K

S

G

G

A

A

V

I

E

A

K

K

A

A

N

E

E

E

L

L

S

A

E

K

S

T

L

D

D

Q

N

R

S

Y

I

F

V

V

L

P

Q

Q

Q
|
Q

F

F

A

E

N

N

E

P

A

A

N

N

P

P

D

A

I

I

H

H

R

R

K

V

T

T

A

A

L

E

E

E

I

V

L

W

R

R

D

D

T

T

D

D

D

G

K

K

V

V

D

D

V

I

L

V

V

V

I

A

A

G

I

V

G

G

T

T

G

G

S

T

I

I

T

T

G

G

I

V

S

A

E

Q

V

V

V

I

K

K

A

E

K

R

N

K

P

P

N

S

V

A

K

R

V

F

I

V

A

A

V

V

E

E

P

P

T

A

D

A

S

S

P

P

V

V

L

L

S

S

G

G

N

Q

P
x
K

G

G

P

P

H

H

K

P

I

I

Q

Q

G
|
G

I

I

G

G

A
|
A

G

G

F

F

V

V

P

P

A

P

V

V

L

L

N

D

T

Q

K

D

I

L

Y

V

D

D

E

E

I

I

T

T

V

V

S

G

N

N

D

E

D

D

A

A

M

L

E

N

T

V

A

A

R

R

N

R

L

L

A

A

K

R

D

E

E

E

G

G

L

L

V

V

G

G

I

I

S
|
S

A

S

G

G

A

A

N

A

V

T

F

V

A

A

S

A

T

L

A

Q

L

V

A

A

Q

R

R

R

E

P

E

E

Y

N

K

A

G

G

K

K

T

L

I

I

V

V

T

V

I

V

L

L

C
x
P

D

D

T

F

G

G

E

E

R

R

Y

Y

L

L

active site: K44 (= K43), S266 (= S265), P293 (≠ C292)
binding : T71 (= T70), S72 (= S71), G73 (= G72), T75 (= T74), M122 (= M121), Q144 (= Q143), K215 (≠ P214), G222 (= G221), A225 (= A224)

7n2tA O-acetylserine sulfhydrylase from citrullus vulgaris in the internal aldimine state, with citrate bound (see paper)
56% identity, 98% coverage: 4:305/309 of query aligns to 4:307/309 of 7n2tA

query
sites
7n2tA

A

A

N

K

D

D

V

V

T

T

E

E

L

L

I

I

G

G

N

N

T

T

P

P

L

L

V

V

K

Y

L

L

K

N

K

R

A

V

S

-

T

V

N

D

G

G

T

C

T

V

-

A

-

R

V

V

L

A

G

A

K

K

C

L

E

E

F

M

M

M

N

E

P

P

T

C

H

S

S

S

V

V

K

K

D

D

R

R

I

I

G

G

F

Y

N

S

M

M

I

I

N

S

E

D

A

A

I

E

K

N

S

K

G

G

K

L

I

I

T

T

E

P

G

G

T

E

T

S

V

V

-

L

I

I

E

E

P

P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

G

L

L

A

A

S

F

V

I

C

A

A

A

K

K

G

G

I

Y

K

R

L

L

I

I

L

I

T

C

M

M

P

P

S

A

S

S

M

M

S

S

I

L

E

E

R

R

K

T

L

I

L

L

K

R

A

A

L

F

G

G

A

A

Q

E

I

L

V

V

L

L

T

T

E

D

P

P

E

A

K

R

G

G

M

M

K

K

G

G

A

A

V

V

E

Q

K

K

A

A

N

E

E

E

L

I

S

K

E

A

S

K

L

T

D

P

N

N

S

S

I

Y

V

I

L

L

Q

Q

F

F

A

E

N

N

E

P

A

A

N

N

P

P

D

K

I

I

H

H

R

Y

K

E

T

T

A

G

L

P

E

E

I

I

L

W

R

R

D

G

T

S

D

G

K

K

V

I

D

D

V

A

L

L

V

V

I

S

A

G

I

I

G

G

T

T

G

G

S

T

I

V

T

T

G

G

I

A

S

G

E

K

V

Y

V

L

K

K

A

E

K

Q

N

N

P

P

N

N

V

I

K

K

V

L

I

Y

A

G

V

V

E

E

P

P

T

V

D

E

S

S

P

A

V

I

L

L

S

S

G

G

N

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

A

G

V

V

L

L

N

D

T

V

K

N

I

L

Y

L

D

D

E

E

I

V

I

I

T

Q

V

V

S

S

N

S

D

E

A

S

M

I

E

E

T

T

A

A

R

K

N

L

L

L

A

A

K

L

D

K

E

E

G

G

L

L

V

V

G

G

I

I

S

S

A

S

G

G

A

A

N

A

V

A

F

A

A

A

S

A

T

I

A
x
R

L

I

A

A

Q

K

R
|
R

E

P

E

E

Y

N

K

A

G

G

K

K

T

L

I

I

V

V

T

A

I

V

L

F

C

P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

V

L

L

Y

F

Q

E

binding phosphate ion: R277 (≠ A275), R281 (= R279)

4aecA Crystal structure of the arabidopsis thaliana o-acetyl-serine-(thiol)- lyasE C (see paper)
54% identity, 99% coverage: 1:305/309 of query aligns to 11:317/323 of 4aecA

query
sites
4aecA

M

L

K

N

Y

I

A

A

N

D

D

N

V

V

T

S

E

Q

L

L

I

I

G

G

N

K

T

T

P

P

L

M

V

V

K

Y

L

L

K

N

K

S

A

I

S

A

T

K

N

G

G

C

T

V

T

A

-

N

V

I

L

A

G

A

K

K

C

L

E

E

F

I

M

M

N

E

P

P

T

C

H

C

S

S

V

V

K
|
K

D

D

R

R

I

I

G

G

F

Y

N

S

M

M

I

V

N

T

E

D

A

A

I

E

K

Q

S

K

G

G

K

F

I

I

T

S

E

P

G

G

T

K

T

S

V

V

-

L

I

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

G

L

L

A

A

S

F

V

I

C

A

A

A

A

S

K

R

G

G

I

Y

K

R

L

L

I

I

L

L

T

T

M

M

P

P

S

A

S

S

M

M

S

S

I

M

E

E

R

R

K

V

L

L

K

K

A

A

L

F

G

G

A

A

Q

E

I

L

V

V

L

L

T

T

E

D

P

P

E

A

K

K

G

G

M

M

K

T

G

G

A

A

V

V

E

Q

K

K

A

A

N

E

E

E

L

I

S

L

E

K

S

N

L

T

D

P

N

D

S

A

I

Y

V

M

L

L

Q

Q

F

F

A

D

N

N

E

P

A

A

N

N

P

P

D

K

I

I

H

H

R

Y

K

E

T

T

A

G

L

P

E

E

I

I

L

W

R

D

D

D

T

T

D

K

D

G

K

K

V

V

D

D

V

I

L

F

V

V

I

A

A

G

I
|
I

G
|
G

T
|
T

G
|
G

S
x
T

I

I

T

T

G

G

I

V

S

G

E

R

V

F

V

I

K

K

A

E

K

K

N

N

P

P

N

K

V

T

K

Q

V

V

I

I

A

G

V

V

E

E

P

P

T

T

D

E

S

S

P

D

V

I

L

L

S

S

G

G

N

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

A

K

V

N

L

L

N

D

T

Q

K

K

I

I

Y

M

D

D

E

E

I

V

I

I

T

A

V

I

S

S

N

S

D

E

A

A

M

I

E

E

T

T

A

A

R

K

N

Q

L

L

A

A

K

L

D

K

E

E

G

G

L

L

L

M

V

V

G

G

I

I

S
|
S

A

S

G

G

A

A

N

A

V

A

F

A

A

A

S

A

T

I

A

K

L

V

A

A

Q

K

R

R

E

P

E

E

Y

N

K

A

G

G

K

K

T

L

I

I

V

A

T

V

I

V

L

F

C
x
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

P

L

L

Y

F

Q

Q

active site: K54 (= K43), S277 (= S265)
binding pyridoxal-5'-phosphate: K54 (= K43), N85 (= N73), I188 (= I176), G189 (= G177), T190 (= T178), G191 (= G179), G192 (= G180), T193 (≠ S181), G233 (= G221), S277 (= S265), P304 (≠ C292)

4lmbA Crystal structure analysis of o-acetylserine sulfhydrylase cysk2 complexed with cystine from microcystis aeruginosa 7806 (see paper)
53% identity, 99% coverage: 1:305/309 of query aligns to 1:309/310 of 4lmbA

query
sites
4lmbA

M

M

K

L

Y

I

A

A

N

R

D

D

V

I

T

T

E

Q

L

L

I

V

G

G

N

R

T

T

P

P

L

L

V

V

K

Q

L

L

K

N

K

R

-

I

-

P

-

V

A

A

S

E

T

G

N

V

G

K

T

A

T

R

V

I

L

V

G

V

K

K

C

L

E

E

F

S

M

M

N

N

P

P

T

A

H

A

S

S

V

V

K
|
K

D

D

R

R

I

I

G

G

F

V

N

S

M

M

I

V

N

E

E

D

A

A

I

E

K

A

S

A

G

G

K

L

I

I

-

H

T

P

E

D

G

K

T

T

T

I

V

L

I

V

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

S

M

V

V

C

A

A

A

K

K

G

G

I

Y

K

R

L

L

I

V

L

L

T

T

M

M

P

P

S

E

S

T

M
|
M

S

S

I

L

E

E

R

R

K

A

L

M

L

L

K

K

A

A

L

Y

G

G

A

A

Q

Q

I

L

V

E

L

L

T

T

E

P

P

G

E

S

K

Q

G

G

M
|
M

K

E

G

G

A

A

V

I

E

T

K

R

A

A

N

E

E

E

L

I

S

V

E

E

S

N

L

T

D

P

N

N

S

A

I

Y

V

S

L

L

Q

Q

Q
|
Q

F
|
F

A

R

N

N

E

P

A

A

N

N

P

P

D

K

I

I

H

H

R

R

K

E

T

T

A

A

L

E

E

E

I

I

L

W

R

A

D

D

T

T

D

D

D

G

K

L

V

V

D

D

V

I

L

V

V

I

I

G

A

G

I
x
V

G
|
G

T
|
T

G
|
G

G

G

S
x
T

I

I

T

T

G

G

I

I

S

A

E

E

V

T

V

I

K

K

A

P

K

R

N

R

P

P

N

Q

V

F

K

Q

V

A

I

I

A

A

V

V

E

E

P

P

T

S

D

N

S

S

P

P

V

V

L

L

S

S

G

G

N

Q

P

P

G

G

P

P

H

H

K

K

I

I

Q
|
Q

G
|
G

I
|
I

G

G

A
|
A

G

G

F

F

V

I

P

P

A

A

V

I

L

F

N

R

T

P

K

E

I

L

Y

I

D

D

E

E

I

V

I

I

T

I

V

V

S

D

N

D

D

T

D

E

A

A

M

F

E

A

T

Y

A

A

R

R

N

R

L

L

A

A

K

R

D

Q

E

E

G

G

L

L

V

S

G

G

I

I

S
|
S

A

A

G

G

A

A

N

A

V

L

F

W

A

A

S

A

T

I

A

Q

L

V

A

G

Q

K

R

R

E

P

E

E

Y

N

K

E

G

D

K

K

T

L

I

I

V

V

T

M

I

I

L

Q

C
x
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

A

L

L

Y

F

Q

K

active site: K46 (= K43), S269 (= S265)
binding cysteine: K46 (= K43), T74 (= T70), S75 (= S71), N77 (= N73), T78 (= T74), M101 (= M97), M125 (= M121), M125 (= M121), Q147 (= Q143), F148 (= F144), Q224 (= Q220), G225 (= G221), G225 (= G221), I226 (= I222), A228 (= A224)
binding pyridoxal-5'-phosphate: K46 (= K43), N77 (= N73), V180 (≠ I176), G181 (= G177), T182 (= T178), G183 (= G179), T185 (≠ S181), G225 (= G221), S269 (= S265), P296 (≠ C292)

Q93244 Cysteine synthase 1; O-acetylserine (thiol)-lyase 1; OAS-TL; EC 2.5.1.47 from Caenorhabditis elegans (see 2 papers)
57% identity, 95% coverage: 10:304/309 of query aligns to 14:310/341 of Q93244

query
sites
Q93244

L

L

I

I

G

G

N

N

T

T

P

P

L

M

V

V

K

Y

L

I

K

N

K

K

A

L

S

-

T

T

N

K

G

G

T

L

-

P

-

G

T

T

V

V

L

A

G

V

K

K

C

I

E

E

F

Y

M

M

N

N

P

P

T

A

H

G

S

S

V

V

K

K

D

D

R

R

I

I

G

G

F

A

N

A

M

M

I

L

N

A

E

A

A

A

I

E

K

K

S

D

G

G

K

T

I

V

T

I

E

P

G

G

-

V

T

T

V

L

I

I

E

E

P
|
P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

A

L

L

A

A

S

F

V

V

C
x
A

A

A

K

K

G

G

I

Y

K

R

L

C

I

I

L

V

T

T

M

M

P

P

S

A

S

S

M

M

S

S

I

G

E

E

R

R

K

T

L

L

K

K

A

A

L

Y

G

G

A

S

Q

E

I

V

V

V

L

L

T

T

E

D

P

P

E

A

K

K

G

G

M

M

K

K

G

G

A

A

V

I

E

D

K

M

A

A

N

N

E

Q

L

L

S

K

E

E

S

N

L

I

D

P

N

G

S
|
S

I

I

V

I

L

L

Q

A

Q

Q

F

F

A

D

N

N

E

P

A

N

N

N

P

P

D

L

I

V

H

H

R

Y

K

Q

T

T

A

G

L

P

E

E

I

I

L

W

R

R

D

Q

T

T

D

K

D

G

K

T

V

V

D

D

V

A

L

V

V

V

I

F

A
x
G

I

V

G
|
G

T

T

G

G

S

T

I

I

T

T

G

G

I

V

S

G

E

R

V

Y

V

L

K

Q

A

E

K

Q

N

N

P

P

N

G

V

V

K

R

V

V

I

F

A

A

V

V

E

E

P

P

T

E

D

E

S

S

P

A

V

I

L

L

S

S

G

G

G

R

N

P

P

A

G

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G
|
G

A

A

G

G

F

F

V

A

P

P

A

A

V

V

L

L

N

D

T

T

K

K

I

I

Y

Y

D

E

E

D

I

V

I

I

T

R

V

I

S

H

N

S

D

D

D

E

A

A

M

I

E

V

T

M

A

A

R

Q

N
x
R

L

L

A

S

K

Y

D

E

E

E

G

G

L

L

V

G

G

G

I

I

S

S

A
x
S

G

G

A

A

N

N

V

V

F

A

A

A

S

A

T

L

A

Q

L

L

A

A

Q

A

R

R

E

P

E

E

Y

M

K

A

G

G

K

K

T

L

I

I

V

V

T
|
T

I

C

L

L

C

P

D

S

T

C

G

G

E

E

R

R

Y

Y

L

M

S

T

T

S

P

P

L

L

Y

Y

P75 (= P69) mutation to L: In n5537; severe loss of protein stability.
A88 (≠ C82) mutation to V: In n5522; severe loss of protein stability.
S144 (= S138) mutation to F: In mr26; susceptible to high levels of hydrogen sulfide.
G181 (≠ A175) mutation to E: In n5521 and mr23; severe loss of protein stability. Susceptible to high levels of hydrogen sulfide.
G183 (= G177) mutation to R: In n5515; severe loss of protein stability.
G229 (= G223) mutation to E: In mr33; susceptible to high levels of hydrogen sulfide.
R259 (≠ N253) mutation to K: In n5519; no loss of protein stability. No effect on enzyme activity.
S272 (≠ A266) mutation to F: In mr29; susceptible to high levels of hydrogen sulfide.
T295 (= T289) mutation to I: In mr39; susceptible to high levels of hydrogen sulfide.

P47998 Cysteine synthase 1; At.OAS.5-8; Beta-substituted Ala synthase 1;1; ARAth-Bsas1;1; CSase A; AtCS-A; Cys-3A; O-acetylserine (thiol)-lyase 1; OAS-TL A; O-acetylserine sulfhydrylase; Protein ONSET OF LEAF DEATH 3; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
56% identity, 98% coverage: 2:304/309 of query aligns to 4:308/322 of P47998

query
sites
P47998

K

R

Y

I

A

A

N

K

D

D

V

V

T

T

E

E

L

L

I

I

G

G

N

N

T

T

P

P

L

L

V

V

K

Y

L

L

K

N

A

V

S

A

T

E

N

G

G

C

T

V

-

G

T

R

V

V

L

A

G

A

K

K

C

L

E

E

F

M

M

M

N

E

P

P

T

C

H

S

S

S

V

V

K
|
K

D

D

R

R

I

I

G

G

F

F

N

S

M

M

I

I

N

S

E

D

A

A

I

E

K

K

S

K

G

G

K

L

I

I

T

K

E

P

G

G

T

E

T

S

V

V

-

L

I

I

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

S

F

V

T

C

A

A

A

K

K

G

G

I

Y

K

K

L

L

I

I

L

I

T

T

M

M

P

P

S

A

S

S

M

M

S

S

I

T

E

E

R

R

K

I

L

I

L

L

K

L

A

A

L

F

G

G

A

V

Q

E

I

L

V

V

L

L

T

T

E

D

P

P

E

A

K

K

G

G

M

M

K

K

G

G

A

A

V

I

E

A

K

K

A

A

N

E

E

E

L

I

S

L

E

A

S

K

L

T

D

P

N

N

S

G

I

Y

V

M

L

L

Q

Q

Q
|
Q

F

F

A

E

N

N

E

P

A

A

N

N

P

P

D

K

I

I

H
|
H

R

Y

K

E

T

T

A
x
G

L

P

E

E

I

I

L

W

R

K

D

G

T

T

D

G

K

K

V

I

D

D

V

G

L

F

V

V

I

S

A

G

I

I

G
|
G

T
|
T

G
|
G

S
x
T

I

I

T

T

G

G

I

A

S

G

E

K

V

Y

V

L

K

K

A

E

K

Q

N

N

P

A

N

N

V

V

K

K

V

L

I

Y

A

G

V

V

E

E

P

P

T

V

D

E

S

S

P

A

V

I

L

L

S

S

G

G

N
x
K

P

P

G

G

P

P

H
|
H

K
|
K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

A

S

V

V

L

L

N

N

T

V

K

D

I

L

Y

I

D

D

E

E

I

V

T

Q

V

V

S

S

N

S

D

D

D

E

A

S

M

I

E

D

T

M

A

A

R

R

N

Q

L

L

A

A

K

L

D

K

E

E

G

G

L

L

V

V

G

G

I

I

S
|
S

A

S

G

G

A

A

N

A

V

A

F

A

A

A

S

A

T

I

A

K

L

L

A

A

Q

Q

R

R

E

P

E

E

Y

N

K

A

G

G

K

K

T

L

I

F

V

V

T

A

I

I

L

F

C

P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

V

L

L

Y

F

K46 (= K43) modified: N6-(pyridoxal phosphate)lysine; mutation to A: No cysteine synthase activity.
T74 (= T70) mutation to A: Strong reduction of cysteine synthase activity.; mutation to S: Reduction of cysteine synthase activity.
S75 (= S71) mutation S->A,N,T: Strong reduction of cysteine synthase activity.
N77 (= N73) binding pyridoxal 5'-phosphate; mutation to A: Reduction of cysteine synthase activity.; mutation to D: Strong reduction of cysteine synthase activity.
T78 (= T74) mutation T->A,S: Reduction of cysteine synthase activity.
Q147 (= Q143) mutation Q->A,E: Strong reduction of cysteine synthase activity.
H157 (= H153) mutation H->Q,N: Slight reduction of cysteine synthase activity.
G162 (≠ A158) mutation to E: In old3-1; displays a early leaf death phenotype. Abolishes cysteine synthase activity.
GTGGT 181:185 (≠ GTGGS 177:181) binding pyridoxal 5'-phosphate
T182 (= T178) mutation T->A,S: Slight reduction of cysteine synthase activity.
T185 (≠ S181) mutation T->A,S: Strong reduction of cysteine synthase activity.
K217 (≠ N213) mutation to A: Impaired interaction with SAT1.
H221 (= H217) mutation to A: Impaired interaction with SAT1.
K222 (= K218) mutation to A: Impaired interaction with SAT1.
S269 (= S265) binding pyridoxal 5'-phosphate; mutation to A: Strong reduction of cysteine synthase activity.; mutation to T: Reduction of cysteine synthase activity.

2isqA Crystal structure of o-acetylserine sulfhydrylase from arabidopsis thaliana in complex with c-terminal peptide from arabidopsis serine acetyltransferase (see paper)
56% identity, 98% coverage: 2:304/309 of query aligns to 2:306/320 of 2isqA

query
sites
2isqA

K

R

Y

I

A

A

N

K

D

D

V

V

T

T

E

E

L

L

I

I

G

G

N

N

T

T

P

P

L

L

V

V

K

Y

L

L

K

N

A

V

S

A

T

E

N

G

G

C

T

V

-

G

T

R

V

V

L

A

G

A

K

K

C

L

E

E

F

M

M

M

N

E

P

P

T

C

H

S

S

S

V

V

K
|
K

D

D

R

R

I

I

G

G

F

F

N

S

M

M

I

I

N

S

E

D

A

A

I

E

K

K

S

K

G

G

K

L

I

I

T

K

E

P

G

G

T

E

T

S

V

V

-

L

I

I

E

E

P

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

S

F

V

T

C

A

A

A

K

K

G

G

I

Y

K

K

L

L

I

I

L

I

T

T

M

M

P

P

S

A

S

S

M

M

S

S

I

T

E

E

R

R

K

I

L

I

L

L

K

L

A

A

L

F

G

G

A

V

Q

E

I

L

V

V

L

L

T

T

E

D

P

P

E

A

K

K

G
|
G

M
|
M

K
|
K

G

G

A

A

V

I

E

A

K

K

A

A

N

E

E

E

L

I

S

L

E

A

S

K

L

T

D

P

N

N

S

G

I

Y

V

M

L

L

Q

Q

F

F

A

E

N

N

E

P

A

A

N

N

P

P

D

K

I

I

H

H

R

Y

K

E

T

T

A

G

L

P

E

E

I

I

L

W

R

K

D

G

T

T

D

G

K

K

V

I

D

D

V

G

L

F

V

V

I

S

A
x
G

I

I

G
|
G

T
|
T

G
|
G

G

G

S
x
T

I

I

T

T

G

G

I

A

S

G

E

K

V

Y

V

L

K

K

A

E

K

Q

N

N

P

A

N

N

V

V

K

K

V

L

I

Y

A

G

V

V

E

E

P

P

T

V

D

E

S

S

P

A

V

I

L

L

S

S

G

G

N

K

P

P

G
|
G

P
|
P

H
|
H

K

K

I

I

Q
|
Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

A

S

V

V

L

L

N

N

T

V

K

D

I

L

Y

I

D

D

E

E

I

V

T

Q

V

V

S

S

N

S

D

D

D

E

A

S

M

I

E

D

T

M

A

A

R

R

N

Q

L

L

A

A

K

L

D

K

E

E

G

G

L

L

V

V

G

G

I

I

S
|
S

A

S

G

G

A

A

N

A

V

A

F

A

A

A

S

A

T

I

A

K

L

L

A

A

Q

Q

R

R

E

P

E

E

Y

N

K

A

G

G

K

K

T

L

I

F

V

V

T

A

I

I

L

F

C
x
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

V

L

L

Y

F

active site: K44 (= K43), S267 (= S265)
binding pyridoxal-5'-phosphate: K44 (= K43), N75 (= N73), G177 (≠ A175), G179 (= G177), T180 (= T178), G181 (= G179), T183 (≠ S181), G223 (= G221), S267 (= S265), P294 (≠ C292)
binding : T72 (= T70), S73 (= S71), G74 (= G72), T76 (= T74), G122 (= G120), M123 (= M121), K124 (= K122), G217 (= G215), P218 (= P216), H219 (= H217), Q222 (= Q220), G223 (= G221)

P47999 Cysteine synthase, chloroplastic/chromoplastic; At.OAS.7-4; Beta-substituted Ala synthase 2;1; ARAth-Bsas2;1; CSase B; AtCS-B; CS-B; O-acetylserine (thiol)-lyase; O-acetylserine sulfhydrylase; OAS-TL B; cpACS1; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
55% identity, 98% coverage: 4:305/309 of query aligns to 76:379/392 of P47999

query
sites
P47999

A

A

N

D

D

N

V

A

T

A

E

Q

L

L

I

I

G

G

N

K

T

T

P

P

L

M

V

V

K

Y

L

L

K

N

A

V

S

V

T

K

N

G

G

C

-

V

T

A

T

S

V

V

L

A

G

A

K

K

C

L

E

E

F

I

M

M

N

E

P

P

T

C

H

C

S

S

V

V

K

K

D

D

R

R

I

I

G

G

F

Y

N

S

M

M

I

I

N

T

E

D

A

A

I

E

K

E

S

K

G

G

K

L

I

I

T

T

E

P

G

G

T

K

T

S

V

V

-

L

I

V

E

E

P

S

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

G

L

L

A

A

S

F

V

I

C

A

A

A

A

S

K

K

G

G

I

Y

K

K

L

L

I

I

L

L

T

T

M

M

P

P

S

A

S

S

M

M

S

S

I

L

E

E

R

R

K

V

L

L

K

R

A

A

L

F

G

G

A

A

Q

E

I

L

V

V

L

L

T

T

E

E

P

P

E

A

K

K

G

G

M

M

K

T

G

G

A

A

V

I

E

Q

K

K

A

A

N

E

E

E

L

I

S

L

E

K

S

K

L

T

D

P

N

N

S

S

I

Y

V

M

L

L

Q

Q

F

F

A

D

N

N

E

P

A

A

N

N

P

P

D

K

I

I

H

H

R

Y

K

E

T

T

A

G

L

P

E

E

I

I

L

W

R

E

D

D

T

T

D

R

D

G

K

K

V

I

D

D

V

I

L

L

V

V

I

A

A

G

I

I

G

G

T

T

G

G

S

T

I

I

T

T

G

G

I

V

S

G

E

R

V

F

V

I

K

K

A

E

K

R

N

K

P

P

N

E

V

L

K

K

V

V

I

I

A

G

V

V

E

E

P

P

T

T

D

E

S

S

P

A

V

I

L

L

S

S

G

G

N

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

V

P

P

A

K

V

N

L

L

N

D

T

L

K

A

I

I

Y

V

D

D

E

E

I

Y

I

I

T

A

V

I

S

S

N

S

D

E

A

A

M

I

E

E

T

T

A

S

R

K

N

Q

L

L

A

A

K

L

D

Q

E

E

G

G

L

L

V

V

G

G

I

I

S

S

A

S

G

G

A

A

N

A

V

A

F

A

A

A

S

A

T

I

A

Q

L

V

A

A

Q

K

R

R

E

P

E

E

Y

N

K

A

G

G

K

K

T

L

I

I

V

A

T

V

I

V

L

F

C

P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

Q

L

L

Y

F

Q

Q

Sites not aligning to the query:

61 modified: N-acetylalanine

1z7yA Crystal structure of the arabidopsis thaliana o-acetylserine sulfhydrylase k46a mutant (see paper)
56% identity, 98% coverage: 2:304/309 of query aligns to 2:306/320 of 1z7yA

query
sites
1z7yA

K

R

Y

I

A

A

N

K

D

D

V

V

T

T

E

E

L

L

I

I

G

G

N

N

T

T

P

P

L

L

V

V

K

Y

L

L

K

N

A

V

S

A

T

E

N

G

G

C

T

V

-

G

T

R

V

V

L

A

G

A

K

K

C

L

E

E

F

M

M

M

N

E

P

P

T

C

H

S

S

S

V

V

K
x
A

D

D

R

R

I

I

G

G

F

F

N

S

M

M

I

I

N

S

E

D

A

A

I

E

K

K

S

K

G

G

K

L

I

I

T

K

E

P

G

G

T

E

T

S

V

V

-

L

I

I

E

E

P

P

T

T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

S

F

V

T

C

A

A

A

K

K

G

G

I

Y

K

K

L

L

I

I

L

I

T

T

M

M

P

P

S

A

S

S

M

M

S

S

I

T

E

E

R

R

K

I

L

I

L

L

K

L

A

A

L

F

G

G

A

V

Q

E

I

L

V

V

L

L

T

T

E

D

P

P

E

A

K

K

G

G

M

M

K

K

G

G

A

A

V

I

E

A

K

K

A

A

N

E

E

E

L

I

S

L

E

A

S

K

L

T

D

P

N

N

S

G

I

Y

V

M

L

L

Q

Q

Q
|
Q

F

F

A

E

N

N

E

P

A

A

N

N

P

P

D

K

I

I

H

H

R

Y

K

E

T

T

A

G

L

P

E

E

I

I

L

W

R

K

D

G

T

T

D

G

K

K

V

I

D

D

V

G

L

F

V

V

I

S

A

G

I
|
I

G
|
G

T
|
T

G
|
G

G

G

S
x
T

I

I

T

T

G

G

I

A

S

G

E

K

V

Y

V

L

K

K

A

E

K

Q

N

N

P

A

N

N

V

V

K

K

V

L

I

Y

A

G

V

V

E

E

P

P

T

V

D

E

S

S

P

A

V

I

L

L

S

S

G

G

N

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

A

S

V

V

L

L

N

N

T

V

K

D

I

L

Y

I

D

D

E

E

I

V

T

Q

V

V

S

S

N

S

D

D

D

E

A

S

M

I

E

D

T

M

A

A

R

R

N

Q

L

L

A

A

K

L

D

K

E

E

G

G

L

L

V

V

G

G

I

I

S
|
S

A

S

G

G

A

A

N

A

V

A

F

A

A

A

S

A

T

I

A

K

L

L

A

A

Q

Q

R

R

E

P

E

E

Y

N

K

A

G

G

K

K

T

L

I

F

V

V

T

A

I

I

L

F

C
x
P

D
x
S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

V

L

L

Y

F

active site: A44 (≠ K43), S267 (= S265)
binding n-[(3-hydroxy-2-methyl-5-{[(trihydroxyphosphoranyl)oxy]methyl}pyridin-4-yl)methylene]methionine: G74 (= G72), N75 (= N73), T76 (= T74), Q145 (= Q143), I178 (= I176), G179 (= G177), T180 (= T178), G181 (= G179), T183 (≠ S181), G223 (= G221), S267 (= S265), P294 (≠ C292), S295 (≠ D293)

5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
54% identity, 99% coverage: 1:305/309 of query aligns to 2:307/310 of 5xoqA

query
sites
5xoqA

M

M

K

P

Y

I

A

F

N

K

D

D

V

N

T

S

E

E

L

S

I

I

G

G

N

R

T

T

P

P

L

L

V

V

K

Q

L

I

K

N

K

R

A

L

S

T

T

A

N

G

-

L

G

S

T

S

T

R

V

V

L

L

G

A

K

K

C

I

E

E

F

G

M

R

N

N

P

P

T

A

H

Y

S

S

V

V

K

K

D

C

R

R

I

I

G

G

F

A

N

A

M

M

I

I

N

W

E

D

A

A

I

E

K

Q

S

S

G

G

K

K

I

L

T

K

E

P

G

G

T

M

T

H

V

V

I

V

E

E

P

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

A

A

L

L

A

A

S

F

V

V

C

C

A

A

K

R

G

G

I

Y

K

K

L

L

I

T

L

L

T

T

M

M

P

P

S

E

S

T

M

M

S

S

I

I

E

E

R

R

K

M

L

M

L

L

K

K

A

S

L

F

G

G

A

A

Q

D

I

L

V

V

L

L

T

T

E

P

P

G

E

A

K

D

G

G

M
|
M

K

K

G

G

A

A

V

I

E

S

K

K

A

A

N

E

E

E

L

L

S

A

E

-

S

A

L

Q

D

P

N

G

S

W

I

F

V

I

L

P

Q

Q

Q
|
Q

F

F

A

K

N

N

E

P

A

A

N

N

P

P

D

A

I

I

H

H

R

V

K

K

T

T

A

G

L

P

E

E

I

I

L

W

R

N

D

D

T

T

D

E

D

G

K

Q

V

V

D

D

V

V

L

F

V

V

I

A

A

G

I

V

G

G

T

T

G

G

S

T

I

I

T

T

G

G

I

V

S

A

E

R

V

F

V

L

K

K

A

H

-

E

-

K

K

K

N

H

P

P

N

-

V

V

K

H

V

V

I

V

A

A

V

V

E

E

P

P

T

A

D

A

S

S

P

P

V

V

L

L

S

A

G

G

N

P

P

A

G

G

P
x
R

H
|
H

K

K

I

I

Q
|
Q

G
|
G

I

I

G

G

A
|
A

G

G

F

F

V

V

P

P

A

D

V

T

L

F

N

D

T

R

K

S

I

V

Y

V

D

D

E

E

I

I

I

L

T

S

V

V

S

T

N

D

D

D

D

E

A

A

M

I

E

E

T

T

A

A

R

R

N

K

L

L

A

A

K

M

D

E

E

E

G

G

L

I

L

S

V

C

G

G

I

I

S

S

A

C

G

G

A

A

N

A

V

M

F

A

A

G

S

A

T

L

A

K

L

V

A

A

Q

A

R

R

E

P

E

E

Y

F

K

A

G

G

K

K

T

T

I

I

V

V

T

T

I

V

L

L

C

P

D

D

T

A

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

A

L

L

Y

F

Q

E

binding : T72 (= T70), S73 (= S71), G74 (= G72), T76 (= T74), M123 (= M121), Q144 (= Q143), R218 (≠ P216), H219 (= H217), Q222 (= Q220), G223 (= G221), A226 (= A224)

P0ABK5 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; S-carboxymethylcysteine synthase; Sulfate starvation-induced protein 5; SSI5; EC 2.5.1.47; EC 4.5.1.5 from Escherichia coli (strain K12) (see 5 papers)
54% identity, 98% coverage: 2:304/309 of query aligns to 3:312/323 of P0ABK5

query
sites
P0ABK5

K

K

Y

I

A

F

N

E

D

D

V

N

T

S

E

L

L

T

I

I

G

G

N

H

T

T

P

P

L

L

V

V

K

R

L

L

K

N

K

R

A

I

S

G

T

-

N

N

G

G

T

R

T

-

V

I

L

L

G

A

K

K

C

V

E

E

F

S

M

R

N

N

P

P

T

S

H

F

S

S

V

V

K
|
K

D

C

R

R

I

I

G

G

F

A

N

N

M

M

I

I

N

W

E

D

A

A

I

E

K

K

S

R

G

G

K

V

I

L

T

K

E

P

G

G

T

V

T

E

V

L

I

V

E

E

P

P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

A

L

L

A

A

S

Y

V

V

C

A

A

A

K

R

G

G

I

Y

K

K

L

L

I

T

L

L

T

T

M

M

P

P

S

E

S

T

M

M

S

S

I

I

E

E

R

R

K

K

L

L

K

K

A

A

L

L

G

G

A

A

Q

N

I

L

V

V

L

L

T

T

E

E

P

G

E

A

K

K

G

G

M

M

K

K

G

G

A

A

V

I

E

Q

K

K

A

A

N

E

E

E

L

I

-

V

S

A

E

S

S

N

L

P

D

E

N

K

S

Y

I

L

V

L

L

L

Q

Q

F

F

A

S

N

N

E

P

A

A

N

N

P

P

D

E

I

I

H

H

R

E

K

K

T

T

A

G

L

P

E

E

I

I

L

W

R

E

D

D

T

T

D

D

D

G

K

Q

V

V

D

D

V

V

L

F

V

I

I

A

A

G

I

V

G

G

T

T

G

G

S

T

I

L

T

T

G

G

I

V

S

S

E

R

V

Y

V

I

K

K

A

G

K

T

N

K

P

G

N

K

V

T

K

D

V

L

I

I

-

S

-

V

A

A

V

V

E

E

P

P

T

T

D

D

S

S

P

P

V

V

L

I

S

A

G

Q

G

A

-

L

-

A

-

G

-

E

-

I

N

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

A

A

V

N

L

L

N

D

T

L

K

K

I

L

Y

V

D

D

E

K

I

V

I

I

T

G

V

I

S

T

N

N

D

E

A

A

M

I

E

S

T

T

A

A

R

R

N

R

L

L

A

M

K

E

D

E

E

E

G

G

L

I

L

L

V

A

G

G

I

I

S

S

A

S

G

G

A

A

N

A

V

V

F

A

A

A

S

A

T

L

A

K

L

L

A

Q

Q

E

R

D

E

E

E

S

Y

F

K

T

G

N

K

K

T

N

I

I

V

V

T

V

I

I

L

L

C

P

D

S

T

S

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

A

L

L

Y

F

K42 (= K43) modified: N6-(pyridoxal phosphate)lysine; mutation to A: Still stimulates tRNase activity of CdiA-CT in vitro and in vivo.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

P0A1E3 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; EC 2.5.1.47 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
53% identity, 98% coverage: 2:304/309 of query aligns to 3:312/323 of P0A1E3

query
sites
P0A1E3

K

K

Y

I

A

Y

N

E

D

D

V

N

T

S

E

L

L

T

I

I

G

G

N

H

T

T

P

P

L

L

V

V

K

R

L

L

K

N

K

R

A

I

S

G

T

-

N

N

G

G

T

R

T

-

V

I

L

L

G

A

K

K

C

V

E

E

F

S

M

R

N

N

P

P

T

S

H

F

S

S

V

V

K

K

D

C

R

R

I

I

G

G

F

A

N

N

M

M

I

I

N

W

E

D

A

A

I

E

K

K

S

R

G

G

K

V

I

L

T

K

E

P

G

G

T

V

T

E

V

L

I

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

S

Y

V

V

C

A

A

A

K

R

G

G

I

Y

K

K

L

L

I

T

L

L

T

T

M

M

P

P

S

E

S

T

M

M

S

S

I

I

E

E

R

R

K

K

L

L

K

K

A

A

L

L

G

G

A

A

Q

N

I

L

V

V

L

L

T

T

E

E

P

G

E

A

K

K

G

G

M

M

K

K

G

G

A

A

V

I

E

Q

K

K

A

A

N

E

E

E

L

I

S

V

E

A

S

S

-

D

L

P

D

Q

N

K

S

Y

I

L

V

L

L

L

Q

Q

F

F

A

S

N

N

E

P

A

A

N

N

P

P

D

E

I

I

H

H

R

E

K

K

T

T

A

G

L

P

E

E

I

I

L

W

R

E

D

D

T

T

D

D

D

G

K

Q

V

V

D

D

V

V

L

F

V

I

I

S

A

G

I

V

G

G

T

T

G

G

S

T

I

L

T

T

G

G

I

V

S

T

E

R

V

Y

V

I

K

K

A

G

K

T

N

K

P

G

N

K

V

T

K

D

V

L

I

I

-

T

-

V

A

A

V

V

E

E

P

P

T

T

D

D

S

S

P

P

V

V

L

I

S

A

G

Q

G

A

-

L

-

A

-

G

-

E

-

I

N

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

A

G

V

N

L

L

N

D

T

L

K

K

I

L

Y

I

D

D

E

K

I

V

T

G

V

I

S

T

N

N

D

E

A

A

M

I

E

S

T

T

A

A

R

R

N

R

L

L

A

M

K

E

D

E

E

E

G

G

L

I

L

L

V

A

G

G

I

I

S
|
S

A

S

G

G

A

A

N

A

V

V

F

A

A

A

S

A

T

L

A

K

L

L

A

Q

Q

E

R

D

E

E

E

S

Y

F

K

T

G

N

K

K

T

N

I

I

V

V

T

V

I

I

L

L

C

P

D

S

T

S

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

A

L

L

Y

F

N72 (= N73) binding pyridoxal 5'-phosphate
S273 (= S265) binding pyridoxal 5'-phosphate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

8b9wA Cysteine synthase from trypanosoma theileri with plp bound (see paper)
51% identity, 97% coverage: 4:304/309 of query aligns to 10:311/329 of 8b9wA

query
sites
8b9wA

A

A

N

P

D

S

V

V

T

D

E

A

L

L

I

I

G

G

N

N

T

T

P

P

L

A

V

V

K

Y

L

L

K

R

K

N

A

M

S

N

T

K

N

T

G

A

T

A

T

T

V

I

L

V

G

L

K

K

C

L

E

E

F

S

M

E

N

N

P

P

T

M

H

A

S

S

V

V

K
|
K

D

D

R

R

I

L

G

A

F

Y

N

A

M

I

I

Y

N

D

E

K

A

A

I

E

K

K

S

E

G

G

K

K

I

I

T

K

E

R

G

G

T

K

T

S

V

V

I

I

-

V

E

E

P

A

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

S

L

V

L

C

G

A

A

A

V

K

R

G

G

I

Y

K

K

L

V

I

I

L

I

T

T

M

M

P

P

S

D

S

S

M

M

S

S

I

M

E

E

R

R

K

S

L

L

K

R

A

I

L

F

G

G

A

A

Q

E

I

L

V

I

L

L

T

T

E

P

P

A

E

S

K

L

G

G

M

M

K

K

G

G

A

A

V

V

E

A

K

M

A

A

N

N

E

R

L

I

S

V

E

S

S

N

L

N

D

P

N

D

S

A

I

V

V

L

L

A

Q

D

Q

Q

F

F

A

C

N

T

E

K

A

Y

N

N

P

A

D

Q

I

I

H

H

R

E

K

E

T

T

A

G

L

P

E

E

I

I

L

W

R

R

D

Q

T

T

D

K

D

G

K

H

V

V

D

D

V

C

L

F

V

V

I

A

A

G

I

V

G
|
G

T
|
T

G
|
G

G

G

S
x
T

I

L

T

T

G

G

I

V

S

A

E

R

V

F

V

L

K

K

A

S

K

V

N

G

P

C

N

N

V

A

K

N

V

I

I

V

A

A

V

V

E

E

P

P

T

Q

D

E

S

S

P

P

V

V

L

L

S

S

G

G

N

R

P

P

G

G

P

P

H

H

K

R

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

V

P

P

A

E

V

V

L

L

N

D

T

R

K

E

I

L

Y

V

D

D

E

E

I

I

T

Q

V

V

S

S

N

S

D

D

D

K

A

A

M

I

E

E

T

T

A

A

R

Q

N

K

L

L

A

S

K

R

D

M

E

D

G

G

L

I

L

F

V

C

G

G

I

F

S
|
S

A

A

G

G

A

A

N

N

V

V

F

F

A

A

S

A

T

L

A

K

L

I

A

A

Q

E

R

R

E

P

E

E

Y

M

K

A

G

G

K

K

T

T

I

I

V

V

T

T

I

V

L

I

C
x
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

A

L

L

Y

Y

binding pyridoxal-5'-phosphate: K49 (= K43), N80 (= N73), G184 (= G177), T185 (= T178), G186 (= G179), T188 (≠ S181), G228 (= G221), S272 (= S265), P299 (≠ C292)

3vbeC Crystal structure of beta-cyanoalanine synthase in soybean (see paper)
51% identity, 97% coverage: 6:305/309 of query aligns to 14:315/329 of 3vbeC

query
sites
3vbeC

D

H

V

V

T

S

E

Q

L

L

I

I

G

G

N

R

T

T

P

P

L

L

V

V

K

Y

L

L

K

N

K

K

A

V

S

T

T

E

N

G

-

C

G

G

T

A

T

Y

V

V

L

A

G

V

K

K

C

Q

E

E

F

M

M

M

N

Q

P

P

T

T

H

A

S

S

V

I

K
|
K

D

D

R

R

I

P

G

A

F

Y

N

A

M

M

I

I

N

T

E

D

A

A

I

E

K

E

S

K

G

N

K

L

I

I

T

T

E

P

G

G

-

K

T

T

V

L

I

I

E

E

P

P

T

T

S
|
S

G

G

N
|
N

T

M

G

G

I

I

A

S

L

M

A

A

S

F

V

M

C

A

A

A

A

M

K

K

G

G

I

Y

K

K

L

M

I

V

L

L

T

T

M

M

P

P

S

S

S

Y

M

T

S

S

I

L

E

E

R

R

K

V

L

T

L

M

K

R

A

A

L

F

G

G

A

A

Q

E

I

L

V

I

L

L

T

T

E

D

P

P

E

A

K

K

G

G

M

M

K

G

G

G

A

T

V

V

E

K

K

K

A

A

N

Y

E

E

L

L

S

L

E

E

S

N

L

T

D

P

N

N

S

A

I

H

V

M

L

L

Q

Q

F

F

A

S

N

N

E

P

A

A

N

N

P

T

D

Q

I

V

H

H

R

F

K

E

T

T

A

G

L

P

E

E

I

I

L

W

R

E

D

D

T

T

D

N

D

G

K

Q

V

V

D

D

V

I

L

F

V

V

I
x
M

A

G

I

I

G
|
G

T
x
S

G
|
G

G

G

S
x
T

I

V

T

S

G

G

I

V

S

G

E

Q

V

Y

V

L

K

K

A

S

K

K

N

N

P

P

N

N

V

V

K

K

V

I

I

Y

A

G

V

V

E
|
E

P

P

T

S

D

E

S
|
S

P

N

V

V

L

L

S

N

G

G

N

K

P

P

G

G

P

P

H

H

K

H

I

I

Q

T

G
|
G

I

N

G

G

A

V

G

G

F

F

V

K

P

P

A

D

V

I

L

L

N

D

T

L

K

D

I

V

Y

M

D

E

E

K

I

V

I

L

T

E

V

V

S

S

N

S

D

E

D

D

A

A

M

V

E

N

T

M

A

A

R

R

N

V

L

L

A

A

K

L

D

K

E

E

G

G

L

L

L

M

V

V

G

G

I

I

S
|
S

A

S

G

G

A

A

N

N

V

T

F

V

A

A

S

A

T

L

A

R

L

L

A

A

Q

Q

R

L

E

P

E

E

Y

N

K

K

G

G

K

K

T

L

I

I

V

V

T

T

I

V

L

H

C
x
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

S

P

V

L

L

Y

F

Q

Q

active site: K52 (= K43), S81 (= S71), E212 (= E202), S216 (= S206), S275 (= S265), P302 (≠ C292)
binding pyridoxal-5'-phosphate: K52 (= K43), N83 (= N73), M184 (≠ I174), G187 (= G177), S188 (≠ T178), G189 (= G179), T191 (≠ S181), G231 (= G221), S275 (= S265), P302 (≠ C292)

6z4nAAA structure of oass complexed with upar inhibitor (see paper)
53% identity, 98% coverage: 2:304/309 of query aligns to 4:313/321 of 6z4nAAA

query
sites
6z4nAAA

K

K

Y

I

A

Y

N

E

D

D

V

N

T

S

E

L

L

T

I

I

G

G

N

H

T

T

P

P

L

L

V

V

K

R

L

L

K

N

K

R

A

I

S

G

T

-

N

N

G

G

T

R

T

-

V

I

L

L

G

A

K

K

C

V

E

E

F

S

M

R

N

N

P

P

T

S

H

F

S

S

V

V

K
|
K

D

C

R

R

I

I

G

G

F

A

N

N

M

M

I

I

N

W

E

D

A

A

I

E

K

K

S

R

G

G

K

V

I

L

T

K

E

P

G

G

T

V

T

E

V

L

I

V

E

E

P

P

T
|
T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

S

Y

V

V

C

A

A

A

K

R

G

G

I

Y

K

K

L

L

I

T

L

L

T

T

M

M

P

P

S

E

S

T

M

M

S

S

I

I

E

E

R

R

K

K

L

L

K

K

A

A

L

L

G

G

A

A

Q

N

I

L

V

V

L

L

T

T

E

E

P

G

E

A

K

K

G

G

M

M

K

K

G

G

A

A

V

I

E

Q

K

K

A

A

N

E

E

E

L

I

S

V

E

A

S

S

-

D

L

P

D

Q

N

K

S

Y

I

L

V

L

L

L

Q

Q

Q
|
Q

F
|
F

A

S

N

N

E

P

A

A

N

N

P

P

D

E

I

I

H

H

R

E

K

K

T

T

A

G

L

P

E

E

I

I

L

W

R

E

D

D

T

T

D

D

D

G

K

Q

V

V

D

D

V

V

L

F

V

I

I

S

A

G

I
x
V

G
|
G

T
|
T

G
|
G

G

G

S
x
T

I

L

T

T

G

G

I

V

S

T

E

R

V

Y

V

I

K

K

A

G

K

T

N

K

P

G

N

K

V

T

K

D

V

L

I

I

-

T

-

V

A

A

V

V

E

E

P

P

T

T

D

D

S

S

P

P

V

V

L

I

S

A

G

Q

G

A

-

L

-

A

-

G

-

E

-

I

N

K

P

P

G

G

P

P

H

H

K

K

I

I

Q
|
Q

G
|
G

I
|
I

G

G

A
|
A

G

G

F

F

V

I

P

P

A

G

V

N

L

L

N

D

T

L

K

K

I

L

Y

I

D

D

E

K

I

V

T

G

V

I

S

T

N

N

D

E

A

A

M

I

E

S

T

T

A

A

R

R

N

R

L

L

A

M

K

E

D

E

E

E

G

G

L

I

L

L

V

A

G

G

I

I

S
|
S

A

S

G

G

A

A

N

A

V

V

F

A

A

A

S

A

T

L

A

K

L

L

A

Q

Q

E

R

D

E

E

E

S

Y

F

K

T

G

N

K

K

T

N

I

I

V

V

T

V

I

I

L

L

C
x
P

D

S

T

S

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

P

A

L

L

Y

F

binding pyridoxal-5'-phosphate: K43 (= K43), N73 (= N73), V177 (≠ I176), G178 (= G177), T179 (= T178), G180 (= G179), T182 (≠ S181), G230 (= G221), S274 (= S265), P301 (≠ C292)
binding (1~{S},2~{S})-1-[(4-methylphenyl)methyl]-2-phenyl-cyclopropane-1-carboxylic acid: K43 (= K43), T70 (= T70), G72 (= G72), N73 (= N73), T74 (= T74), Q144 (= Q143), F145 (= F144), Q229 (= Q220), G230 (= G221), I231 (= I222), A233 (= A224)

Q9FS29 Bifunctional L-3-cyanoalanine synthase/cysteine synthase 2, mitochondrial; EC 2.5.1.47; EC 4.4.1.9 from Solanum tuberosum (Potato) (see paper)
51% identity, 97% coverage: 5:305/309 of query aligns to 30:332/347 of Q9FS29

query
sites
Q9FS29

N

S

D

E

V

V

T

S

E

Q

L

L

I

I

G

G

N

K

T

T

P

P

L

M

V

V

K

Y

L

L

K

K

A

V

S

T

T

E

N

G

-

C

G

G

T

A

T

Y

V

I

L

A

G

V

K

K

C

Q

E

E

F

M

M

F

N

Q

P

P

T

T

H

S

S

S

V

I

K

K

D

D

R

R

I

P

G

A

F

L

N

A

M

M

I

I

N

N

E

D

A

A

I

E

K

K

S

K

G

G

K

L

I

I

T

S

-

P

E

E

G

K

T

T

V

L

I

I

E

E

P

P

T

T

S

S

G

G

N

N

T

M

G

G

I

I

A

S

L

M

A

A

S

F

V

M

C

A

A

A

A

M

K

K

G

G

I

Y

K

K

L

M

I

V

L

L

T

T

M

M

P

P

S

S

S

Y

M

T

S

S

I

M

E

E

R

R

K

V

L

T

L

M

K

R

A

A

L

F

G

G

A

A

Q

D

I

L

V

I

L

L

T

T

E

D

P

P

E

T

K

K

G

G

M

M

K

G

G

G

A

T

V

V

E

K

K

K

A

A

N

Y

E
|
E

L

L

S

L

E

E

S

S

L

T

D

P

N

N

S

A

I

F

V

M

L

L

Q

Q

F

F

A

S

N

N

E

P

A

A

N

N

P

T

D

Q

I

V

H

H

R

F

K

D

T

T

A

G

L

P

E

E

I

I

L

W

R

E

D

E

T

T

D

L

D

G

K

N

V

V

D

D

V

I

L

F

V

V

I

M

A

G

I

I

G

G

T

S

G

G

S

T

I

V

T

T

G

G

I

V

S

G

E

L

V

Y

V

L

K

K

A

S

K

K

N

N

P

P

N

N

V

V

K

K

V

I

I

Y

A

G

V

L

E

E

P

P

T

T

D

E

S

S

P

N

V

I

L

L

S

N

G

G

N

K

P

P

G

G

P

P

H

H

K

H

I

I

Q

T

G

G

I

N

G

G

A

V

G

G

F

S

V

K

P

P

A

D

V

I

L

V

N

D

T

M

K

D

I

L

Y

M

D

E

E

E

I

V

I

L

T

M

V

V

S

S

N

S

D

E

D

D

A

A

M

V

E

N

T

M

A

A

R

R

N

E

L

L

A

A

K

V

D

K

E

E

G

G

L

L

L

M

V

V

G

G

I

I

S

S

A

S

G

G

A

A

N

N

V

T

F

V

A

A

S

A

T

L

A

R

L

L

A

A

Q

Q

R

K

E

P

E

E

Y

N

K

K

G

G

K

K

T

L

I

I

V

V

T

T

I

V

L

H

C

A

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

S

P

V

L

L

Y

Y

Q

Q

E157 (= E130) mutation E->N,Q: No effect on catalytic activities.

Query Sequence

>WP_013136878.1 NCBI__GCF_000092245.1:WP_013136878.1
MKYANDVTELIGNTPLVKLKKASTNGTTVLGKCEFMNPTHSVKDRIGFNMINEAIKSGKI
TEGTTVIEPTSGNTGIALASVCAAKGIKLILTMPSSMSIERRKLLKALGAQIVLTEPEKG
MKGAVEKANELSESLDNSIVLQQFANEANPDIHRKTTALEILRDTDDKVDVLVIAIGTGG
SITGISEVVKAKNPNVKVIAVEPTDSPVLSGGNPGPHKIQGIGAGFVPAVLNTKIYDEII
TVSNDDAMETARNLAKDEGLLVGISAGANVFASTALAQREEYKGKTIVTILCDTGERYLS
TPLYQFDDE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory