SitesBLAST
Comparing WP_013256866.1 NCBI__GCF_000143965.1:WP_013256866.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
2pg0A Crystal structure of acyl-coa dehydrogenase from geobacillus kaustophilus
43% identity, 97% coverage: 5:377/386 of query aligns to 4:376/380 of 2pg0A
- active site: M124 (≠ V127), T125 (= T128), E243 (= E244), A364 (= A365), R376 (= R377)
- binding flavin-adenine dinucleotide: I122 (≠ L125), M124 (≠ V127), T125 (= T128), G130 (= G133), S131 (= S134), F155 (= F158), I156 (= I159), T157 (= T160), R269 (= R270), F272 (= F273), F279 (≠ L280), Q337 (= Q338), L338 (≠ F339), G340 (= G341), G341 (= G342), V359 (≠ L360), I362 (= I363), Y363 (≠ V364), T366 (= T367), E368 (= E369), M369 (≠ V370)
3r7kA Crystal structure of a probable acyl coa dehydrogenase from mycobacterium abscessus atcc 19977 / dsm 44196 (see paper)
44% identity, 97% coverage: 5:377/386 of query aligns to 4:375/378 of 3r7kA
- active site: V126 (= V127), T127 (= T128), E242 (= E244), G363 (≠ A365), K375 (≠ R377)
- binding dihydroflavine-adenine dinucleotide: V126 (= V127), T127 (= T128), G132 (= G133), S133 (= S134), F157 (= F158), I158 (= I159), T159 (= T160), R268 (= R270), T270 (≠ A272), F271 (= F273), L275 (= L277), R278 (≠ L280), I281 (≠ L283), Q336 (= Q338), I337 (≠ F339), G340 (= G342), I358 (≠ L360), T365 (= T367), E367 (= E369)
8w0uA Human lcad complexed with acetoacetyl coenzyme a (see paper)
38% identity, 96% coverage: 6:377/386 of query aligns to 21:392/398 of 8w0uA
- binding acetoacetyl-coenzyme a: M140 (≠ V127), S147 (= S134), Q150 (≠ A137), S193 (≠ A178), H196 (≠ R181), Y250 (= Y235), E259 (= E244), R260 (= R245), Y379 (≠ V364), G380 (≠ A365), G381 (= G366), I385 (≠ V370), L389 (≠ I374), R392 (= R377)
- binding flavin-adenine dinucleotide: I138 (≠ L125), M140 (≠ V127), T141 (= T128), G146 (= G133), S147 (= S134), F171 (= F158), S173 (≠ T160), R285 (= R270), F288 (= F273), L295 (= L280), Q353 (= Q338), L354 (≠ F339), G357 (= G342), V375 (≠ L360), Y379 (≠ V364), T382 (= T367), E384 (= E369)
8w0tA Human lcad (see paper)
38% identity, 96% coverage: 6:377/386 of query aligns to 21:392/398 of 8w0tA
- binding flavin-adenine dinucleotide: I138 (≠ L125), M140 (≠ V127), T141 (= T128), G146 (= G133), S147 (= S134), F171 (= F158), I172 (= I159), S173 (≠ T160), R285 (= R270), F288 (= F273), L295 (= L280), Q353 (= Q338), L354 (≠ F339), G356 (= G341), G357 (= G342), V375 (≠ L360), T382 (= T367), E384 (= E369), I385 (≠ V370)
P28330 Long-chain specific acyl-CoA dehydrogenase, mitochondrial; LCAD; EC 1.3.8.8 from Homo sapiens (Human) (see 2 papers)
38% identity, 96% coverage: 6:377/386 of query aligns to 53:424/430 of P28330
- E291 (= E244) active site, Proton acceptor; mutation to Q: Loss of long-chain-acyl-CoA dehydrogenase activity. No effect on protein abundance. No effect on solubility. No effect on substrate binding.
- S303 (≠ A256) to T: in dbSNP:rs1801204
- K333 (≠ R286) to Q: in dbSNP:rs2286963
3p4tA Crystal structure of a putative acyl-coa dehydrogenase from mycobacterium smegmatis (see paper)
41% identity, 97% coverage: 3:377/386 of query aligns to 1:375/381 of 3p4tA
- active site: I125 (≠ V127), T126 (= T128), E241 (= E244), G363 (≠ A365), K375 (≠ R377)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-[(4aS,10aR)-7,8-dimethyl-2,4-dioxo-1,3,4,4a,5,10a-hexahydrobenzo[g]pteridin-10(2H)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate: L123 (= L125), I125 (≠ V127), T126 (= T128), G130 (≠ A132), G131 (= G133), S132 (= S134), Y156 (≠ F158), I157 (= I159), T158 (= T160), K200 (= K203), R267 (= R270), T269 (≠ A272), L274 (= L277), R277 (≠ L280), Q336 (= Q338), L337 (≠ F339), G340 (= G342), I358 (≠ L360), T365 (= T367)
3oibA Crystal structure of a putative acyl-coa dehydrogenase from mycobacterium smegmatis, iodide soak (see paper)
41% identity, 97% coverage: 3:377/386 of query aligns to 1:375/381 of 3oibA
- active site: I125 (≠ V127), T126 (= T128), E241 (= E244), G363 (≠ A365), K375 (≠ R377)
- binding dihydroflavine-adenine dinucleotide: L123 (= L125), I125 (≠ V127), T126 (= T128), G131 (= G133), S132 (= S134), Y156 (≠ F158), I157 (= I159), T158 (= T160), K200 (= K203), R267 (= R270), T269 (≠ A272), L274 (= L277), R277 (≠ L280), Q336 (= Q338), L337 (≠ F339), G340 (= G342), I358 (≠ L360), T365 (= T367), E367 (= E369)
5lnxD Crystal structure of mmgc, an acyl-coa dehydrogenase from bacillus subtilis.
39% identity, 96% coverage: 13:381/386 of query aligns to 9:374/374 of 5lnxD
- active site: L122 (≠ V127), T123 (= T128), G239 (≠ E244), E358 (≠ A365), K370 (≠ R377)
- binding flavin-adenine dinucleotide: L122 (≠ V127), T123 (= T128), G128 (= G133), S129 (= S134), F153 (= F158), T155 (= T160), R265 (= R270), Q267 (≠ A272), F268 (= F273), I272 (≠ L277), N275 (≠ L280), I278 (≠ L283), Q331 (= Q338), I332 (≠ F339), G335 (= G342), Y357 (≠ V364), T360 (= T367), E362 (= E369)
P51174 Long-chain specific acyl-CoA dehydrogenase, mitochondrial; LCAD; EC 1.3.8.8 from Mus musculus (Mouse) (see paper)
38% identity, 96% coverage: 6:377/386 of query aligns to 53:424/430 of P51174
- K318 (≠ N271) modified: N6-acetyllysine; mutation to R: Reduces activity by 37%; reduces activity by 80% when associated with R-322.
- K322 (≠ A275) modified: N6-acetyllysine; alternate; mutation to R: Reduces activity by 23%; reduces activity by 80% when associated with R-318.
Sites not aligning to the query:
- 42 modified: N6-acetyllysine; K→R: Reduces activity by 90% when associated with R-318 and R-322.
4l1fA Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
39% identity, 93% coverage: 19:377/386 of query aligns to 18:375/380 of 4l1fA
- active site: L125 (≠ V127), T126 (= T128), G242 (≠ E244), E363 (≠ A365), R375 (= R377)
- binding coenzyme a persulfide: T132 (≠ S134), H179 (≠ R181), F232 (= F234), M236 (= M238), E237 (≠ Q239), L239 (≠ F241), D240 (≠ Q242), R243 (= R245), Y362 (≠ V364), E363 (≠ A365), G364 (= G366), R375 (= R377)
- binding flavin-adenine dinucleotide: F123 (≠ L125), L125 (≠ V127), T126 (= T128), G131 (= G133), T132 (≠ S134), F156 (= F158), I157 (= I159), T158 (= T160), R268 (= R270), Q270 (≠ A272), F271 (= F273), I275 (≠ L277), F278 (≠ L280), L281 (= L283), Q336 (= Q338), I337 (≠ F339), G340 (= G342), I358 (≠ L360), Y362 (≠ V364), T365 (= T367), Q367 (≠ E369)
Sites not aligning to the query:
5ol2F The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
39% identity, 92% coverage: 20:376/386 of query aligns to 18:373/378 of 5ol2F
- active site: L124 (≠ V127), T125 (= T128), G241 (≠ E244)
- binding calcium ion: E29 (≠ Q31), E33 (≠ A35), R35 (≠ A37)
- binding coenzyme a persulfide: L238 (≠ F241), R242 (= R245), E362 (≠ A365), G363 (= G366)
- binding flavin-adenine dinucleotide: F122 (≠ L125), L124 (≠ V127), T125 (= T128), P127 (= P130), T131 (≠ S134), F155 (= F158), I156 (= I159), T157 (= T160), E198 (≠ L201), R267 (= R270), F270 (= F273), L274 (= L277), F277 (≠ L280), Q335 (= Q338), L336 (≠ F339), G338 (= G341), G339 (= G342), Y361 (≠ V364), T364 (= T367), E366 (= E369)
Sites not aligning to the query:
1ivhA Structure of human isovaleryl-coa dehydrogenase at 2.6 angstroms resolution: structural basis for substrate specificity (see paper)
36% identity, 96% coverage: 9:377/386 of query aligns to 11:382/387 of 1ivhA
- active site: M130 (≠ V127), S131 (≠ T128), E249 (= E244), A370 (= A365), R382 (= R377)
- binding coenzyme a persulfide: S137 (= S134), S185 (≠ A180), R186 (= R181), V239 (≠ F234), Y240 (= Y235), M243 (= M238), E249 (= E244), R250 (= R245), G369 (≠ V364), A370 (= A365), G371 (= G366), V375 (= V370)
- binding flavin-adenine dinucleotide: L128 (= L125), M130 (≠ V127), S131 (≠ T128), G136 (= G133), S137 (= S134), W161 (≠ F158), T163 (= T160), R275 (= R270), F278 (= F273), F285 (≠ L280), M288 (≠ L283), Q343 (= Q338), C344 (≠ F339), G347 (= G342), T372 (= T367), E374 (= E369)
8sgrA Human liver mitochondrial isovaleryl-coa dehydrogenase (see paper)
36% identity, 96% coverage: 9:377/386 of query aligns to 15:386/393 of 8sgrA
- binding flavin-adenine dinucleotide: S135 (≠ T128), G140 (= G133), S141 (= S134), W165 (≠ F158), T167 (= T160), R279 (= R270), F282 (= F273), I286 (≠ L277), F289 (≠ L280), Q347 (= Q338), C348 (≠ F339), G351 (= G342), L369 (= L360), G375 (= G366), T376 (= T367), L382 (≠ E373)
P26440 Isovaleryl-CoA dehydrogenase, mitochondrial; IVD; Butyryl-CoA dehydrogenase; EC 1.3.8.4; EC 1.3.8.1 from Homo sapiens (Human) (see 5 papers)
36% identity, 96% coverage: 9:377/386 of query aligns to 48:419/426 of P26440
- 165:174 (vs. 125:134, 60% identical) binding FAD
- S174 (= S134) binding substrate
- WIT 198:200 (≠ FIT 158:160) binding FAD
- SR 222:223 (≠ AR 180:181) binding substrate
- G250 (≠ A208) to A: in IVA; uncertain significance
- Y277 (= Y235) binding substrate
- DLER 284:287 (≠ QLER 242:245) binding substrate
- E286 (= E244) active site, Proton acceptor; mutation to D: Residual isovaleryl-CoA dehydrogenase activity.; mutation to G: Loss of isovaleryl-CoA dehydrogenase activity. Does not affect isovaleryl-CoA dehydrogenase activity; when associated with 407-E.; mutation to Q: Loss of isovaleryl-CoA dehydrogenase activity.
- A291 (= A249) to V: in IVA; uncertain significance; dbSNP:rs886042098
- R312 (= R270) binding FAD
- Q323 (= Q281) binding FAD
- I379 (≠ L337) to T: in IVA; uncertain significance
- QCFGG 380:384 (≠ QFFGG 338:342) binding FAD
- R398 (= R356) to Q: in IVA; uncertain significance; dbSNP:rs1477527791
- Y403 (≠ G361) to N: in IVA; uncertain significance
- A407 (= A365) mutation to E: Does not affect isovaleryl-CoA dehydrogenase activity; when associated with 286-D.
- AG 407:408 (= AG 365:366) binding substrate
- TSE 409:411 (= TSE 367:369) binding FAD
Sites not aligning to the query:
- 1:32 modified: transit peptide, Mitochondrion
1ukwB Crystal structure of medium-chain acyl-coa dehydrogenase from thermus thermophilus hb8
37% identity, 96% coverage: 9:377/386 of query aligns to 8:374/379 of 1ukwB
- active site: L124 (≠ V127), S125 (≠ T128), T241 (≠ E244), E362 (≠ A365), R374 (= R377)
- binding cobalt (ii) ion: D145 (= D148), H146 (= H149)
- binding flavin-adenine dinucleotide: F122 (≠ L125), L124 (≠ V127), S125 (≠ T128), G130 (= G133), S131 (= S134), W155 (≠ F158), S157 (≠ T160), K200 (= K203), L357 (= L360), Y361 (≠ V364), E362 (≠ A365), T364 (= T367), E366 (= E369), L370 (≠ E373)
1ukwA Crystal structure of medium-chain acyl-coa dehydrogenase from thermus thermophilus hb8
37% identity, 96% coverage: 9:377/386 of query aligns to 8:374/379 of 1ukwA
- active site: L124 (≠ V127), S125 (≠ T128), T241 (≠ E244), E362 (≠ A365), R374 (= R377)
- binding flavin-adenine dinucleotide: F122 (≠ L125), L124 (≠ V127), S125 (≠ T128), G130 (= G133), S131 (= S134), W155 (≠ F158), S157 (≠ T160), L357 (= L360), Y361 (≠ V364), E362 (≠ A365), T364 (= T367), E366 (= E369), L370 (≠ E373)
P15651 Short-chain specific acyl-CoA dehydrogenase, mitochondrial; SCAD; Butyryl-CoA dehydrogenase; EC 1.3.8.1 from Rattus norvegicus (Rat) (see 2 papers)
36% identity, 97% coverage: 1:376/386 of query aligns to 25:403/412 of P15651
- 152:161 (vs. 125:134, 50% identical) binding FAD
- S161 (= S134) binding substrate
- WIT 185:187 (≠ FIT 158:160) binding FAD
- DMGR 269:272 (≠ QLER 242:245) binding substrate
- R297 (= R270) binding FAD
- QILGG 365:369 (≠ QFFGG 338:342) binding FAD
- E392 (≠ A365) active site, Proton acceptor
- TSE 394:396 (= TSE 367:369) binding FAD
Sites not aligning to the query:
- 1:24 modified: transit peptide, Mitochondrion
1jqiA Crystal structure of rat short chain acyl-coa dehydrogenase complexed with acetoacetyl-coa (see paper)
37% identity, 96% coverage: 8:376/386 of query aligns to 8:376/384 of 1jqiA
- binding acetoacetyl-coenzyme a: L95 (≠ Q93), F125 (≠ L125), S134 (= S134), F234 (= F234), M238 (= M238), Q239 (= Q239), L241 (≠ F241), D242 (≠ Q242), R245 (= R245), Y364 (≠ V364), E365 (≠ A365), G366 (= G366)
- binding flavin-adenine dinucleotide: F125 (≠ L125), L127 (≠ V127), S128 (≠ T128), G133 (= G133), S134 (= S134), W158 (≠ F158), T160 (= T160), R270 (= R270), F273 (= F273), L280 (= L280), Q338 (= Q338), I339 (≠ F339), G342 (= G342), I360 (≠ L360), T367 (= T367), E369 (= E369), I370 (≠ V370)
Sites not aligning to the query:
7szvA Crystal structure of acyl-coa dehydrogenase from mycobacterium marinum in complex with fda
36% identity, 96% coverage: 9:377/386 of query aligns to 5:368/372 of 7szvA
- binding dihydroflavine-adenine dinucleotide: L122 (≠ V127), T123 (= T128), F153 (= F158), I154 (= I159), T155 (= T160), K194 (= K203), R261 (= R270), S263 (≠ A272), Y271 (≠ L280), I274 (≠ L283), Q329 (= Q338), V330 (≠ F339), G332 (= G341), G333 (= G342), T358 (= T367), E360 (= E369)
4n5fA Crystal structure of a putative acyl-coa dehydrogenase with bound fadh2 from burkholderia cenocepacia j2315
36% identity, 96% coverage: 6:377/386 of query aligns to 6:376/378 of 4n5fA
- active site: L126 (≠ V127), T127 (= T128), G243 (≠ E244), E364 (≠ A365), R376 (= R377)
- binding dihydroflavine-adenine dinucleotide: L126 (≠ V127), T127 (= T128), G132 (= G133), S133 (= S134), F157 (= F158), T159 (= T160), T210 (= T211), Y363 (≠ V364), T366 (= T367), E368 (= E369), M372 (≠ E373)
Query Sequence
>WP_013256866.1 NCBI__GCF_000143965.1:WP_013256866.1
MHSIYFGPEHHEFRRNVRRFVENEIAPHADQWEQARAIPRQAFRRMGELGFLGVCFPEEY
GGAEGDIFMAMALLEELARSRMGGFAAAVAVQQFMAPQHIHKYGSEELKRKYLAGSISGE
MVGALGVTEPGAGSDVAAIRAKASRQGDHYLINGAKTFITNGADGHFITLACKTEPGAGA
RGISLIVVDLDAPGVSCTRRLEKMGWHASDTAELTFEDVRAPLANLVGQENKGFYYIMQA
FQLERLACAAMGLGLAQLCLEHAVKYMGERNAFGAPLSNLQALAHRLAELSARQEAARQL
TYHAAWLYQNDLPCVAQCSMAKLLACELAKRVADECLQFFGGYGLMEEYPMARLLRDSRL
GTIVAGTSEVMREIIARLSFGRADYK
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory