SitesBLAST
Comparing WP_013257549.1 NCBI__GCF_000143965.1:WP_013257549.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
O43175 D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; Malate dehydrogenase; EC 1.1.1.95; EC 1.1.1.399; EC 1.1.1.37 from Homo sapiens (Human) (see 3 papers)
45% identity, 99% coverage: 2:315/316 of query aligns to 8:320/533 of O43175
- T78 (= T72) binding NAD(+)
- R135 (≠ K129) to W: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606949
- RI 155:156 (≠ NI 149:150) binding NAD(+)
- D175 (= D169) binding NAD(+)
- T207 (≠ V201) binding NAD(+)
- CAR 234:236 (= CAR 228:230) binding NAD(+)
- D260 (= D254) binding NAD(+)
- V261 (= V255) to M: in PHGDHD; results in a four-fold decrease in substrate affinity and a slight increase in maximal enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs267606947
- HLGA 283:286 (= HLGA 278:281) binding NAD(+)
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
- 373 A → T: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs201553627
- 377 G → S: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606948
- 425 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907988
- 490 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907987
6plfA Crystal structure of human phgdh complexed with compound 1 (see paper)
45% identity, 96% coverage: 2:304/316 of query aligns to 4:305/305 of 6plfA
6plgA Crystal structure of human phgdh complexed with compound 15 (see paper)
45% identity, 96% coverage: 2:303/316 of query aligns to 3:303/303 of 6plgA
7dkmA Phgdh covalently linked to oridonin (see paper)
45% identity, 96% coverage: 2:303/316 of query aligns to 4:304/306 of 7dkmA
- binding nicotinamide-adenine-dinucleotide: T74 (= T72), A102 (= A100), G148 (= G146), R151 (≠ N149), I152 (= I150), Y170 (≠ F168), D171 (= D169), P172 (= P170), I173 (≠ F171), H202 (= H200), T203 (≠ V201), P204 (= P202), T209 (= T207), C230 (= C228), A231 (= A229), R232 (= R230), H279 (= H278), G281 (= G280)
- binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: C14 (≠ K12), K17 (≠ E15), I18 (= I16), E293 (≠ V292)
6rj3A Crystal structure of phgdh in complex with compound 15 (see paper)
45% identity, 94% coverage: 2:298/316 of query aligns to 2:297/297 of 6rj3A
7ewhA Crystal structure of human phgdh in complex with homoharringtonine (see paper)
45% identity, 94% coverage: 2:298/316 of query aligns to 3:298/302 of 7ewhA
- binding (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine: L146 (≠ I145), G147 (= G146), L148 (= L147), G149 (= G148), R150 (≠ N149), I151 (= I150), G152 (= G151), D170 (= D169), H201 (= H200), T202 (≠ V201), P203 (= P202)
6rihA Crystal structure of phgdh in complex with compound 9 (see paper)
45% identity, 94% coverage: 2:298/316 of query aligns to 3:298/302 of 6rihA
6cwaA Crystal structure phgdh in complex with nadh and 3-phosphoglycerate at 1.77 a resolution (see paper)
45% identity, 94% coverage: 2:298/316 of query aligns to 2:297/299 of 6cwaA
- binding 1,4-dihydronicotinamide adenine dinucleotide: N96 (= N96), A100 (= A100), R149 (≠ N149), I150 (= I150), Y168 (≠ F168), D169 (= D169), P170 (= P170), I171 (≠ F171), H200 (= H200), T201 (≠ V201), P202 (= P202), T207 (= T207), C228 (= C228), A229 (= A229), R230 (= R230), H277 (= H278), G279 (= G280)
6rj5A Crystal structure of phgdh in complex with compound 39 (see paper)
45% identity, 94% coverage: 2:298/316 of query aligns to 3:298/301 of 6rj5A
6rj2A Crystal structure of phgdh in complex with compound 40 (see paper)
45% identity, 94% coverage: 3:298/316 of query aligns to 1:295/299 of 6rj2A
- binding ~{N}-[(1~{R})-1-[4-(ethanoylsulfamoyl)phenyl]ethyl]-2-methyl-5-phenyl-pyrazole-3-carboxamide: G146 (= G148), I148 (= I150), Y166 (≠ F168), D167 (= D169), P168 (= P170), I169 (≠ F171), I170 (≠ M172), H198 (= H200), T199 (≠ V201), L208 (= L210), R228 (= R230)
6plfB Crystal structure of human phgdh complexed with compound 1 (see paper)
44% identity, 94% coverage: 2:298/316 of query aligns to 2:289/292 of 6plfB
- binding 4-{(1S)-1-[(5-chloro-6-{[(5S)-2-oxo-1,3-oxazolidin-5-yl]methoxy}-1H-indole-2-carbonyl)amino]-2-hydroxyethyl}benzoic acid: R141 (≠ N149), Y160 (≠ F168), D161 (= D169), P162 (= P170), I164 (≠ M172), L179 (≠ F187), T193 (≠ V201), P194 (= P202), S198 (≠ Q206), L202 (= L210)
1wwkA Crystal structure of phosphoglycerate dehydrogenase from pyrococcus horikoshii ot3
44% identity, 96% coverage: 1:304/316 of query aligns to 1:304/304 of 1wwkA
- active site: S96 (≠ N96), R230 (= R230), D254 (= D254), E259 (= E259), H278 (= H278)
- binding nicotinamide-adenine-dinucleotide: V100 (≠ A100), G146 (= G146), F147 (≠ L147), G148 (= G148), R149 (≠ N149), I150 (= I150), Y168 (≠ F168), D169 (= D169), P170 (= P170), V201 (= V201), P202 (= P202), T207 (= T207), T228 (≠ C228), S229 (≠ A229), D254 (= D254), H278 (= H278), G280 (= G280)
3ddnB Crystal structure of hydroxypyruvic acid phosphate bound d-3- phosphoglycerate dehydrogenase in mycobacterium tuberculosis (see paper)
43% identity, 98% coverage: 3:312/316 of query aligns to 5:312/525 of 3ddnB
3dc2A Crystal structure of serine bound d-3-phosphoglycerate dehydrogenase from mycobacterium tuberculosis (see paper)
43% identity, 98% coverage: 3:312/316 of query aligns to 4:311/526 of 3dc2A
Sites not aligning to the query:
P87228 Putative D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; EC 1.1.1.95; EC 1.1.1.399 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
36% identity, 99% coverage: 1:314/316 of query aligns to 56:380/466 of P87228
- S87 (≠ D31) modified: Phosphoserine
- S258 (≠ T204) modified: Phosphoserine
5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
38% identity, 86% coverage: 33:304/316 of query aligns to 37:314/334 of 5aovA
- active site: L100 (≠ N96), R241 (= R230), D265 (= D254), E270 (= E259), H288 (= H278)
- binding glyoxylic acid: M52 (≠ R48), L53 (≠ S49), L53 (≠ S49), Y74 (≠ A70), A75 (≠ G71), V76 (≠ T72), G77 (= G73), R241 (= R230), H288 (= H278)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (≠ T72), T104 (≠ A100), F158 (≠ L147), G159 (= G148), R160 (≠ N149), I161 (= I150), S180 (≠ D169), R181 (≠ P170), A211 (≠ H200), V212 (= V201), P213 (= P202), T218 (= T207), I239 (≠ C228), A240 (= A229), R241 (= R230), H288 (= H278), G290 (= G280)
7cvpA The crystal structure of human phgdh from biortus.
38% identity, 94% coverage: 2:298/316 of query aligns to 2:252/254 of 7cvpA
- binding nicotinamide-adenine-dinucleotide: G101 (= G146), G103 (= G148), R104 (≠ N149), I105 (= I150), Y123 (≠ F168), D124 (= D169), P125 (= P170), I126 (≠ F171), H155 (= H200), T156 (≠ V201), P157 (= P202), T162 (= T207), C183 (= C228), A184 (= A229), R185 (= R230), H232 (= H278), G234 (= G280)
1ybaA The active form of phosphoglycerate dehydrogenase (see paper)
36% identity, 99% coverage: 1:314/316 of query aligns to 7:324/406 of 1ybaA
- active site: N104 (= N96), R236 (= R230), D260 (= D254), E265 (= E259), H288 (= H278)
- binding 2-oxoglutaric acid: R56 (= R48), S57 (= S49), C79 (≠ G71), I80 (≠ T72)
- binding nicotinamide-adenine-dinucleotide: I80 (≠ T72), F102 (≠ G94), V108 (≠ A100), G154 (= G146), G156 (= G148), H157 (≠ N149), I158 (= I150), Y176 (≠ F168), D177 (= D169), I178 (vs. gap), K181 (vs. gap), H206 (= H200), V207 (= V201), P208 (= P202), A234 (≠ C228), S235 (≠ A229), R236 (= R230), H288 (= H278), G290 (= G280)
- binding phosphate ion: G81 (= G73), N83 (≠ D75)
1psdA The allosteric ligand site in the vmax-type cooperative enzyme phosphoglycerate dehydrogenase (see paper)
36% identity, 99% coverage: 1:314/316 of query aligns to 5:322/404 of 1psdA
- active site: N102 (= N96), R234 (= R230), D258 (= D254), E263 (= E259), H286 (= H278)
- binding nicotinamide-adenine-dinucleotide: N102 (= N96), H155 (≠ N149), I156 (= I150), D175 (= D169), I176 (vs. gap), K179 (vs. gap), H204 (= H200), V205 (= V201), P206 (= P202), A232 (≠ C228), S233 (≠ A229), R234 (= R230), H286 (= H278)
Sites not aligning to the query:
P0A9T0 D-3-phosphoglycerate dehydrogenase; PGDH; 2-oxoglutarate reductase; EC 1.1.1.95; EC 1.1.1.399 from Escherichia coli (strain K12) (see 2 papers)
36% identity, 99% coverage: 1:314/316 of query aligns to 11:328/410 of P0A9T0
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
Query Sequence
>WP_013257549.1 NCBI__GCF_000143965.1:WP_013257549.1
MKILVSDPLHEKGVEIFKNEGFEVEVKTGLDPEALKAAMAGVDGLVIRSATKVTAELLAA
ADSLKVVGRAGTGLDNVDIPACTAKGVIVMNTPGQNSNAAAELAMGHIFAVSRHIGRGNA
GVKQGKWEKKQLRGRELKGKTLGIIGLGNIGRILAELATGCKMSVLGFDPFMDAEAIKAR
GAEPVSFDDLLARSDYVSIHVPKTKQTAGLFNAATFAKMKDGAILINCARGGIVVEEDLC
AALEQGKLAGAALDVFEVEPLPANSRLLYADDVVCTPHLGANTYEAQENVAVAVANQMSR
FLKGGPAEFAVNAPAK
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory