SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013257581.1 NCBI__GCF_000143965.1:WP_013257581.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P23247 Aspartate-semialdehyde dehydrogenase 2; ASA dehydrogenase 2; ASADH 2; Aspartate-beta-semialdehyde dehydrogenase 2; EC 1.2.1.11 from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) (see paper)
54% identity, 99% coverage: 1:336/341 of query aligns to 1:333/337 of P23247

query
sites
P23247

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C132 (= C132) active site, Acyl-thioester intermediate

2r00C Crystal structure of aspartate semialdehyde dehydrogenase ii complexed with asa from vibrio cholerae (see paper)
54% identity, 98% coverage: 2:336/341 of query aligns to 1:332/336 of 2r00C

query
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2r00C

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active site: C131 (= C132), Q158 (= Q159), R237 (= R239), H244 (= H246)
binding 2,2'-oxydiacetic acid: V15 (= V16), N95 (= N96), C131 (= C132), N315 (= N319)

4r54A Complex crystal structure of sp-aspartate-semialdehyde-dehydrogenase with 3-carboxy-ethyl-phthalic acid (see paper)
52% identity, 97% coverage: 5:335/341 of query aligns to 2:340/357 of 4r54A

query
sites
4r54A

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binding 3-(2-carboxyethyl)benzene-1,2-dicarboxylic acid: G72 (= G75), S73 (≠ G76), T94 (≠ S97), S95 (≠ A98), R98 (= R101), K222 (= K217)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G11), T10 (= T13), G11 (= G14), A12 (= A15), V13 (= V16), A35 (= A38), S36 (= S39), R38 (= R41), S39 (= S42), T56 (≠ L59), S70 (= S73), A71 (= A74), G72 (= G75), T75 (= T78), N93 (= N96), T94 (≠ S97), N126 (= N131), C127 (= C132), G160 (= G165), G328 (= G323)

4r41A Complex crystal structure of 4-nitro-2-phosphono-benzoic acid with sp- aspartate-semialdehyde dehydrogenase and nicotinamide-dinucleotide (see paper)
52% identity, 97% coverage: 5:335/341 of query aligns to 2:340/357 of 4r41A

query
sites
4r41A

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E

D

D

P

D

T

S

I

I

K

A

V

V

S

S

A

A

T

T

C

C

V

V

R

R

V

I

P

P

V

V

I

L

Y

S

G

A

H

H

S

S

E

E

A

S

C

V

N

Y

I

I

T

E

T

T

A

K

R

E

K

V

I

A

S

P

A

I

Q

E

A

V

R

K

E

A

A

A

L

I

A

A

K

A

A

F

P

P

G

G

V

A

K

V

V

L

V

E

D

D

P

V

A

A

N

H

K

Q

L

I

Y

Y

P

P

M

Q

P

A

L

I

D

N

A

A

A

V

G

G

Q

S

D

R

L

D

T

T

L

F

V

V

G

G

R

R

I

I

R

R

E

K

D

D

V

L

S

D

Q

A

E

E

K

K

G

G

L

I

D

H

L

M

W

W

L

V

V

V

A

S

D

D

N

N

I

L

R

L

K

K

G

G

A

A

A

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

L

T

L

L

binding 4-nitro-2-phosphonobenzoic acid: S70 (= S73), G72 (= G75), S73 (≠ G76), N93 (= N96), T94 (≠ S97), S95 (≠ A98), R98 (= R101), K222 (= K217)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G11), T10 (= T13), G11 (= G14), A12 (= A15), A35 (= A38), S36 (= S39), R38 (= R41), S39 (= S42), T56 (≠ L59), A71 (= A74), G160 (= G165), M161 (≠ Q166), G162 (≠ K167)

4r3nA Crystal structure of the ternary complex of sp-asadh with NADP and 1, 2,3-benzenetricarboxylic acid (see paper)
52% identity, 97% coverage: 5:335/341 of query aligns to 2:340/357 of 4r3nA

query
sites
4r3nA

Y

Y

R

T

V

V

A

A

V

V

A

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

N

A

Q

Q

M

M

I

I

Q

K

C

M

L

L

E

E

R

S

D

T

F

L

P

P

I

I

G

D

E

K

L

I

R

R

L

Y

L

L

A
|
A

S
|
S

E

A

R

R

S
|
S

R

A

G

G

K

K

R

S

L

L

K

K

Y

F

K

K

G

D

E

Q

D

D

I

I

E

T

V

I

Q

E

V

E

L
x
T

G

T

E

E

G

T

S

A

F

F

Q

E

G

G

V

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

G
x
S

G

S

T

T

S

S

L

A

E

K

W

Y

S

A

P

P

K

Y

A

A

A

V

Q

K

A

A

G

G

A

V

V

V

I

V

D

D

N
|
N

S
x
T

A
x
S

A

Y

W

F

R
|
R

M

Q

D

N

P

P

E

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

H

H

A

A

V

L

A

D

G

A

F

-

K

-

K

H

K

N

G

G

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

I

V

H

R

D

Q

L

K

A

W

G

G

I

L

E

D

R

R

V

I

V

V

S

S

T

T

Y

Y

Q
|
Q

A

A

V

V

S

S

G

G

T

A

G
|
G

Q
x
M

K

G

A

A

I

I

V

L

E

E

L

T

Q

Q

G

R

Q

E

L

L

K

R

A

E

I

V

Y

L

C

N

Q

D

D

G

A

V

Q

K

P

P

-

C

-

D

-

L

Q

H

A

A

K

E

V

I

Y

L

P

P

-

S

-

G

-

D

-

K

-

H

H

Y

V

P

I

I

A

A

Q

F

N

N

V

A

L

L

P

P

H

Q

I

I

D

D

I

V

F

F

L

T

D

D

N

N

G

D

Y

Y

T

T

K

Y

E

E

M

M

K
|
K

M

M

V

T

H

K

E

E

T

T

V

K

K

K

I

I

M

M

E

E

D

D

P

D

T

S

I

I

K

A

V

V

S

S

A

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

I

L

Y

S

G

A

H
|
H

S

S

E

E

A

S

C

V

N

Y

I

I

T

E

T

T

A

K

R

E

K

V

I

A

S

P

A

I

Q

E

A

V

R

K

E

A

A

A

L

I

A

A

K

A

A

F

P

P

G

G

V

A

K

V

V

L

V

E

D

D

P

V

A

A

N

H

K

Q

L

I

Y

Y

P

P

M

Q

P

A

L

I

D

N

A

A

A

V

G

G

Q

S

D

R

L

D

T

T

L

F

V

V

G

G

R

R

I

I

R

R

E

K

D

D

V

L

S

D

Q

A

E

E

K

K

G

G

L

I

D

H

L

M

W

W

L

V

V

V

A

S

D

D

N

N

I

L

R

L

K

K

G
|
G

A

A

A

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

L

T

L

L

active site: C127 (= C132), Q154 (= Q159), R244 (= R239), H251 (= H246)
binding benzene-1,2,3-tricarboxylic acid: S73 (≠ G76), T94 (≠ S97), S95 (≠ A98), R98 (= R101), N126 (= N131), K222 (= K217)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G11), T10 (= T13), G11 (= G14), A12 (= A15), V13 (= V16), A35 (= A38), S36 (= S39), S39 (= S42), T56 (≠ L59), S70 (= S73), A71 (= A74), G72 (= G75), N93 (= N96), T94 (≠ S97), N126 (= N131), C127 (= C132), G160 (= G165), M161 (≠ Q166), G328 (= G323)

3q1lA Crystals structure of aspartate beta-semialdehyde dehydrogenase from streptococcus pneumoniae with cysteamine bound covalently to cys 128 (see paper)
52% identity, 97% coverage: 5:335/341 of query aligns to 2:340/357 of 3q1lA

query
sites
3q1lA

Y

Y

R

T

V

V

A

A

V

V

A

V

G

G

A

A

T

T

G

G

A

A

V

V

G

G

N

A

Q

Q

M

M

I

I

Q

K

C

M

L

L

E

E

R

S

D

T

F

L

P

P

I

I

G

D

E

K

L

I

R

R

L

Y

L

L

A

A

S

S

E

A

R

R

S

S

R

A

G

G

K

K

R

S

L

L

K

K

Y

F

K

K

G

D

E

Q

D

D

I

I

E

T

V

I

Q

E

V

E

L

T

G

T

E

E

G

T

S

A

F

F

Q

E

G

G

V

V

D

D

I

I

A

A

L

L

F

F

S

S

A

A

G

G

G

S

T

T

S

S

L

A

E

K

W

Y

S

A

P

P

K

Y

A

A

A

V

Q

K

A

A

G

G

A

V

V

V

I

V

D

D

N

N

S

T

A

S

A

Y

W

F

R

R

M

Q

D

N

P

P

E

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

H

H

A

A

V

L

A

D

G

A

F

-

K

-

K

H

K

N

G

G

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

I

V

H

R

D

Q

L

K

A

W

G

G

I

L

E

D

R

R

V

I

V

V

S

S

T

T

Y

Y

Q
|
Q

A

A

V

V

S

S

G
|
G

T

A

G

G

Q

M

K

G

A

A

I

I

V

L

E

E

L

T

Q

Q

G

R

Q

E

L

L

K

R

A

E

I

V

Y

L

C

N

Q

D

D

G

A

V

Q

K

P

P

-

C

-

D

-

L

Q

H

A

A

K

E

V

I

Y

L

P

P

-

S

-

G

-

D

-

K

-

H

H

Y

V

P

I

I

A

A

Q

F

N

N

V

A

L

L

P

P

H

Q

I

I

D

D

I

V

F

F

L

T

D

D

N

N

G

D

Y

Y

T

T

K

Y

E
|
E

E

E

M

M

K

K

M

M

V

T

H

K

E

E

T

T

V

K

K

K

I

I

M

M

E

E

D

D

P

D

T

S

I

I

K

A

V

V

S

S

A

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

I

L

Y

S

G

A

H
|
H

S

S

E

E

A

S

C

V

N

Y

I

I

T

E

T

T

A

K

R

E

K

V

I

A

S

P

A

I

Q

E

A

V

R

K

E

A

A

A

L

I

A

A

K

A

A

F

P

P

G

G

V

A

K

V

V

L

V

E

D

D

P

V

A

A

N

H

K

Q

L

I

Y

Y

P

P

M

Q

P

A

L

I

D

N

A

A

A

V

G

G

Q

S

D

R

L

D

T

T

L

F

V

V

G

G

R

R

I

I

R

R

E

K

D

D

V

L

S

D

Q

A

E

E

K

K

G

G

L

I

D

H

L

M

W

W

L

V

V

V

A

S

D

D

N

N

I

L

R

L

K

K

G

G

A

A

A

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

L

T

L

L

active site: C127 (= C132), Q154 (= Q159), R244 (= R239), H251 (= H246)
binding 2-amino-ethanethiol: N126 (= N131), C127 (= C132), G158 (= G163), E219 (= E214)

3pwsA Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with 2',5'-adenosine diphosphate and d-2- aminoadipate (see paper)
52% identity, 97% coverage: 5:335/341 of query aligns to 2:340/357 of 3pwsA

query
sites
3pwsA

Y

Y

R

T

V

V

A

A

V

V

A

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V

V

G

G

N

A

Q

Q

M

M

I

I

Q

K

C

M

L

L

E

E

R

S

D

T

F

L

P

P

I

I

G

D

E

K

L

I

R

R

L

Y

L

L

A
|
A

S
|
S

E

A

R
|
R

S
|
S

R

A

G

G

K

K

R

S

L

L

K

K

Y

F

K

K

G

D

E

Q

D

D

I

I

E

T

V

I

Q

E

V

E

L
x
T

G

T

E

E

G

T

S

A

F

F

Q

E

G

G

V

V

D

D

I

I

A

A

L

L

F

F

S

S

A
|
A

G

G

G

S

T
|
T

S

S

L

A

E

K

W

Y

S

A

P

P

K

Y

A

A

A

V

Q

K

A

A

G

G

A

V

V

V

I

V

D

D

N

N

S

T

A

S

A

Y

W

F

R
|
R

M

Q

D

N

P

P

E

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

H

H

A

A

V

L

A

D

G

A

F

-

K

-

K

H

K

N

G

G

I

I

A

A

N

C

P

P

N
|
N

C

C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

I

V

H

R

D

Q

L

K

A

W

G

G

I

L

E

D

R

R

V

I

V

V

S

S

T

T

Y

Y

Q

Q

A

A

V

V

S

S

G
|
G

T

A

G
|
G

Q
x
M

K

G

A

A

I

I

V

L

E

E

L

T

Q

Q

G

R

Q

E

L

L

K

R

A

E

I

V

Y

L

C

N

Q

D

D

G

A

V

Q

K

P

P

-

C

-

D

-

L

Q

H

A

A

K

E

V

I

Y

L

P

P

-

S

-

G

-

D

-

K

-

H

H

Y

V

P

I

I

A

A

Q

F

N

N

V

A

L

L

P

P

H

Q

I
|
I

D

D

I

V

F

F

L

T

D

D

N

N

G

D

Y

Y

T

T

K

Y

E
|
E

E

E

M

M

K
|
K

M

M

V

T

H

K

E

E

T

T

V

K

K

K

I

I

M

M

E

E

D

D

P

D

T

S

I

I

K

A

V

V

S

S

A

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

I

L

Y

S

G

A

H

H

S

S

E

E

A

S

C

V

N

Y

I

I

T

E

T

T

A

K

R

E

K

V

I

A

S

P

A

I

Q

E

A

V

R

K

E

A

A

A

L

I

A

A

K

A

A

F

P

P

G

G

V

A

K

V

V

L

V

E

D

D

P

V

A

A

N

H

K

Q

L

I

Y

Y

P

P

M

Q

P

A

L

I

D

N

A

A

A

V

G

G

Q

S

D

R

L

D

T

T

L

F

V

V

G

G

R

R

I

I

R

R

E

K

D

D

V

L

S

D

Q

A

E

E

K

K

G

G

L

I

D

H

L

M

W

W

L

V

V

V

A

S

D

D

N

N

I

L

R

L

K

K

G

G

A

A

A

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

L

T

L

L

binding (2R)-2-aminohexanedioic acid: R98 (= R101), N126 (= N131), G158 (= G163), I208 (= I203), E219 (= E214), K222 (= K217), R244 (= R239)
binding adenosine-2'-5'-diphosphate: G8 (= G11), T10 (= T13), G11 (= G14), A12 (= A15), A35 (= A38), S36 (= S39), R38 (= R41), S39 (= S42), T56 (≠ L59), A71 (= A74), T75 (= T78), G160 (= G165), M161 (≠ Q166)

3pwkA Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with 2',5'-adenosine diphosphate and d-2- aminoadipate (see paper)
52% identity, 97% coverage: 5:335/341 of query aligns to 2:340/357 of 3pwkA

query
sites
3pwkA

Y

Y

R

T

V

V

A

A

V

V

A

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V

V

G

G

N

A

Q

Q

M

M

I

I

Q

K

C

M

L

L

E

E

R

S

D

T

F

L

P

P

I

I

G

D

E

K

L

I

R

R

L

Y

L

L

A
|
A

S
|
S

E

A

R
|
R

S
|
S

R

A

G

G

K

K

R

S

L

L

K

K

Y

F

K

K

G

D

E

Q

D

D

I

I

E

T

V

I

Q

E

V

E

L
x
T

G

T

E

E

G

T

S

A

F

F

Q

E

G

G

V

V

D

D

I

I

A

A

L

L

F

F

S

S

A
|
A

G

G

G

S

T
|
T

S

S

L

A

E

K

W

Y

S

A

P

P

K

Y

A

A

A

V

Q

K

A

A

G

G

A

V

V

V

I

V

D

D

N

N

S

T

A

S

A

Y

W

F

R
|
R

M

Q

D

N

P

P

E

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

H

H

A

A

V

L

A

D

G

A

F

-

K

-

K

H

K

N

G

G

I

I

A

A

N

C

P

P

N
|
N

C

C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

I

V

H

R

D

Q

L

K

A

W

G

G

I

L

E

D

R

R

V

I

V

V

S

S

T

T

Y

Y

Q

Q

A

A

V

V

S

S

G
|
G

T
x
A

G
|
G

Q

M

K

G

A

A

I

I

V

L

E

E

L

T

Q

Q

G

R

Q

E

L

L

K

R

A

E

I

V

Y

L

C

N

Q

D

D

G

A

V

Q

K

P

P

-

C

-

D

-

L

Q

H

A

A

K

E

V

I

Y

L

P

P

-

S

-

G

-

D

-

K

-

H

H

Y

V

P

I

I

A

A

Q

F

N

N

V

A

L

L

P

P

H

Q

I

I

D

D

I

V

F

F

L

T

D

D

N

N

G

D

Y

Y

T

T

K

Y

E
|
E

E

E

M

M

K
|
K

M

M

V

T

H

K

E

E

T

T

V

K

K

K

I

I

M

M

E

E

D

D

P

D

T

S

I

I

K

A

V

V

S

S

A

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

I

L

Y

S

G

A

H

H

S

S

E

E

A

S

C

V

N

Y

I

I

T

E

T

T

A

K

R

E

K

V

I

A

S

P

A

I

Q

E

A

V

R

K

E

A

A

A

L

I

A

A

K

A

A

F

P

P

G

G

V

A

K

V

V

L

V

E

D

D

P

V

A

A

N

H

K

Q

L

I

Y

Y

P

P

M

Q

P

A

L

I

D

N

A

A

A

V

G

G

Q

S

D

R

L

D

T

T

L

F

V

V

G

G

R

R

I

I

R

R

E

K

D

D

V

L

S

D

Q

A

E

E

K

K

G

G

L

I

D

H

L

M

W

W

L

V

V

V

A

S

D

D

N

N

I

L

R

L

K

K

G

G

A

A

A

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

L

T

L

L

binding 5'-o-monophosphoryladenylyl(2'->5')adenylyl(2'->5')adenosine: G8 (= G11), T10 (= T13), G11 (= G14), A12 (= A15), A35 (= A38), S36 (= S39), R38 (= R41), S39 (= S42), T56 (≠ L59), A71 (= A74), T75 (= T78), G160 (= G165)
binding trans-cyclohexane-1,4-dicarboxylic acid: R98 (= R101), N126 (= N131), G158 (= G163), A159 (≠ T164), E219 (= E214), K222 (= K217), R244 (= R239)

2gz3A Structure of aspartate semialdehyde dehydrogenase (asadh) from streptococcus pneumoniae complexed with NADP and aspartate- semialdehyde (see paper)
52% identity, 97% coverage: 5:335/341 of query aligns to 2:340/357 of 2gz3A

query
sites
2gz3A

Y

Y

R

T

V

V

A

A

V

V

A

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

N

A

Q

Q

M

M

I

I

Q

K

C

M

L

L

E

E

R

S

D

T

F

L

P

P

I

I

G

D

E

K

L

I

R

R

L

Y

L

L

A
|
A

S
|
S

E

A

R
|
R

S
|
S

R

A

G

G

K

K

R

S

L

L

K

K

Y

F

K

K

G

D

E

Q

D

D

I

I

E

T

V

I

Q

E

V

E

L
x
T

G

T

E

E

G

T

S

A

F

F

Q

E

G

G

V

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

G

S

T
|
T

S

S

L

A

E

K

W

Y

S

A

P

P

K

Y

A

A

A

V

Q

K

A

A

G

G

A

V

V

V

I

V

D

D

N
|
N

S

T

A

S

A

Y

W

F

R

R

M

Q

D

N

P

P

E

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

H

H

A

A

V

L

A

D

G

A

F

-

K

-

K

H

K

N

G

G

I

I

A

A

N

C

P

P

N

N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

I

V

H

R

D

Q

L

K

A

W

G

G

I

L

E

D

R

R

V

I

V

V

S

S

T

T

Y

Y

Q
|
Q

A

A

V

V

S

S

G
|
G

T

A

G
|
G

Q
x
M

K

G

A

A

I

I

V

L

E

E

L

T

Q

Q

G

R

Q

E

L

L

K

R

A

E

I

V

Y

L

C

N

Q

D

D

G

A

V

Q

K

P

P

-

C

-

D

-

L

Q

H

A

A

K

E

V

I

Y

L

P

P

-

S

-

G

-

D

-

K

-

H

H

Y

V

P

I

I

A

A

Q

F

N

N

V

A

L

L

P

P

H

Q

I

I

D

D

I

V

F

F

L

T

D

D

N

N

G

D

Y

Y

T

T

K

Y

E
|
E

E

E

M

M

K

K

M

M

V

T

H

K

E

E

T

T

V

K

K

K

I

I

M

M

E

E

D

D

P

D

T

S

I

I

K

A

V

V

S

S

A

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

I

L

Y

S

G

A

H
|
H

S

S

E

E

A

S

C

V

N

Y

I

I

T

E

T

T

A

K

R

E

K

V

I

A

S

P

A

I

Q

E

A

V

R

K

E

A

A

A

L

I

A

A

K

A

A

F

P

P

G

G

V

A

K

V

V

L

V

E

D

D

P

V

A

A

N

H

K

Q

L

I

Y

Y

P

P

M

Q

P

A

L

I

D

N

A

A

A

V

G

G

Q

S

D

R

L

D

T

T

L

F

V

V

G

G

R

R

I

I

R

R

E

K

D

D

V

L

S

D

Q

A

E

E

K

K

G

G

L

I

D

H

L

M

W

W

L

V

V

V

A

S

D

D

N
|
N

I

L

R

L

K

K

G

G

A
|
A

A

A

A

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

L

T

L

L

active site: C127 (= C132), Q154 (= Q159), R244 (= R239), H251 (= H246)
binding (2r)-2-amino-4-oxobutanoic acid: C127 (= C132), Q154 (= Q159), G158 (= G163), E219 (= E214), R244 (= R239), H251 (= H246)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G11), T10 (= T13), G11 (= G14), A12 (= A15), V13 (= V16), A35 (= A38), S36 (= S39), R38 (= R41), S39 (= S42), T56 (≠ L59), S70 (= S73), A71 (= A74), G72 (= G75), T75 (= T78), N93 (= N96), G158 (= G163), G160 (= G165), M161 (≠ Q166), N324 (= N319), A329 (= A324)

2gz2A Structure of aspartate semialdehyde dehydrogenase (asadh) from streptococcus pneumoniae complexed with 2',5'-adp (see paper)
52% identity, 97% coverage: 5:335/341 of query aligns to 2:340/357 of 2gz2A

query
sites
2gz2A

Y

Y

R

T

V

V

A

A

V

V

A

V

G
|
G

A

A

T
|
T

G
|
G

A

A

V

V

G

G

N

A

Q

Q

M

M

I

I

Q

K

C

M

L

L

E

E

R

S

D

T

F

L

P

P

I

I

G

D

E

K

L

I

R

R

L

Y

L

L

A
|
A

S
|
S

E

A

R
|
R

S
|
S

R

A

G

G

K

K

R

S

L

L

K

K

Y

F

K

K

G

D

E

Q

D

D

I

I

E

T

V

I

Q

E

V

E

L
x
T

G

T

E

E

G

T

S

A

F

F

Q

E

G

G

V

V

D

D

I

I

A

A

L

L

F

F

S

S

A
|
A

G

G

G

S

T
|
T

S

S

L

A

E

K

W

Y

S

A

P

P

K

Y

A

A

A

V

Q

K

A

A

G

G

A

V

V

V

I

V

D

D

N

N

S

T

A

S

A

Y

W

F

R

R

M

Q

D

N

P

P

E

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

H

H

A

A

V

L

A

D

G

A

F

-

K

-

K

H

K

N

G

G

I

I

A

A

N

C

P

P

N

N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

I

V

H

R

D

Q

L

K

A

W

G

G

I

L

E

D

R

R

V

I

V

V

S

S

T

T

Y

Y

Q
|
Q

A

A

V

V

S

S

G

G

T

A

G

G

Q

M

K

G

A

A

I

I

V

L

E

E

L

T

Q

Q

G

R

Q

E

L

L

K

R

A

E

I

V

Y

L

C

N

Q

D

D

G

A

V

Q

K

P

P

-

C

-

D

-

L

Q

H

A

A

K

E

V

I

Y

L

P

P

-

S

-

G

-

D

-

K

-

H

H

Y

V

P

I

I

A

A

Q

F

N

N

V

A

L

L

P

P

H

Q

I

I

D

D

I

V

F

F

L

T

D

D

N

N

G

D

Y

Y

T

T

K

Y

E

E

M

M

K

K

M

M

V

T

H

K

E

E

T

T

V

K

K

K

I

I

M

M

E

E

D

D

P

D

T

S

I

I

K

A

V

V

S

S

A

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

I

L

Y

S

G

A

H
|
H

S

S

E

E

A

S

C

V

N

Y

I

I

T

E

T

T

A

K

R

E

K

V

I

A

S

P

A

I

Q

E

A

V

R

K

E

A

A

A

L

I

A

A

K

A

A

F

P

P

G

G

V

A

K

V

V

L

V

E

D

D

P

V

A

A

N

H

K

Q

L

I

Y

Y

P

P

M

Q

P

A

L

I

D

N

A

A

A

V

G

G

Q

S

D

R

L

D

T

T

L

F

V

V

G

G

R

R

I

I

R

R

E

K

D

D

V

L

S

D

Q

A

E

E

K

K

G

G

L

I

D

H

L

M

W

W

L

V

V

V

A

S

D

D

N

N

I

L

R

L

K

K

G

G

A

A

A

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

L

T

L

L

active site: C127 (= C132), Q154 (= Q159), R244 (= R239), H251 (= H246)
binding adenosine-2'-5'-diphosphate: G8 (= G11), T10 (= T13), G11 (= G14), A35 (= A38), S36 (= S39), R38 (= R41), S39 (= S42), T56 (≠ L59), A71 (= A74), T75 (= T78)

2gz1A Structure of aspartate semialdehyde dehydrogenase (asadh) from streptococcus pneumoniae complexed with NADP (see paper)
52% identity, 97% coverage: 5:335/341 of query aligns to 2:340/357 of 2gz1A

query
sites
2gz1A

Y

Y

R

T

V

V

A

A

V

V

A

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

N

A

Q

Q

M

M

I

I

Q

K

C

M

L

L

E

E

R

S

D

T

F

L

P

P

I

I

G

D

E

K

L

I

R

R

L

Y

L

L

A
|
A

S
|
S

E

A

R
|
R

S
|
S

R

A

G

G

K

K

R

S

L

L

K

K

Y

F

K

K

G

D

E

Q

D

D

I

I

E

T

V

I

Q

E

V

E

L
x
T

G

T

E

E

G

T

S

A

F

F

Q

E

G

G

V

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

G

S

T
|
T

S

S

L

A

E

K

W

Y

S

A

P

P

K

Y

A

A

A

V

Q

K

A

A

G

G

A

V

V

V

I

V

D

D

N
|
N

S

T

A

S

A

Y

W

F

R

R

M

Q

D

N

P

P

E

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

H

H

A

A

V

L

A

D

G

A

F

-

K

-

K

H

K

N

G

G

I

I

A

A

N

C

P

P

N

N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

I

V

H

R

D

Q

L

K

A

W

G

G

I

L

E

D

R

R

V

I

V

V

S

S

T

T

Y

Y

Q
|
Q

A

A

V

V

S
|
S

G
|
G

T

A

G
|
G

Q
x
M

K

G

A

A

I

I

V

L

E

E

L

T

Q

Q

G

R

Q

E

L

L

K

R

A

E

I

V

Y

L

C

N

Q

D

D

G

A

V

Q

K

P

P

-

C

-

D

-

L

Q

H

A

A

K

E

V

I

Y

L

P

P

-

S

-

G

-

D

-

K

-

H

H

Y

V

P

I

I

A

A

Q

F

N

N

V

A

L

L

P

P

H

Q

I

I

D

D

I

V

F

F

L

T

D

D

N

N

G

D

Y

Y

T

T

K

Y

E

E

M

M

K

K

M

M

V

T

H

K

E

E

T

T

V

K

K

K

I

I

M

M

E

E

D

D

P

D

T

S

I

I

K

A

V

V

S

S

A

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

I

L

Y

S

G

A

H
|
H

S

S

E

E

A

S

C

V

N

Y

I

I

T

E

T

T

A

K

R

E

K

V

I

A

S

P

A

I

Q

E

A

V

R

K

E

A

A

A

L

I

A

A

K

A

A

F

P

P

G

G

V

A

K

V

V

L

V

E

D

D

P

V

A

A

N

H

K

Q

L

I

Y

Y

P

P

M

Q

P

A

L

I

D

N

A

A

A

V

G

G

Q

S

D

R

L

D

T

T

L

F

V

V

G

G

R

R

I

I

R

R

E

K

D

D

V

L

S

D

Q

A

E

E

K

K

G

G

L

I

D

H

L

M

W

W

L

V

V

V

A

S

D

D

N
|
N

I
x
L

R

L

K

K

G

G

A

A

A

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

L

T

L

L

active site: C127 (= C132), Q154 (= Q159), R244 (= R239), H251 (= H246)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G11), T10 (= T13), G11 (= G14), A12 (= A15), V13 (= V16), A35 (= A38), S36 (= S39), R38 (= R41), S39 (= S42), T56 (≠ L59), S70 (= S73), A71 (= A74), G72 (= G75), T75 (= T78), N93 (= N96), S157 (= S162), G158 (= G163), G160 (= G165), M161 (≠ Q166), N324 (= N319), L325 (≠ I320)

4r5hA Crystal structure of sp-aspartate-semialdehyde-dehydrogenase with nicotinamide-adenine-dinucleotide-phosphate and 3-carboxy-propenyl- phthalic acid (see paper)
52% identity, 97% coverage: 5:335/341 of query aligns to 2:340/359 of 4r5hA

query
sites
4r5hA

Y

Y

R

T

V

V

A

A

V

V

A

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

N

A

Q

Q

M

M

I

I

Q

K

C

M

L

L

E

E

R

S

D

T

F

L

P

P

I

I

G

D

E

K

L

I

R

R

L

Y

L

L

A
|
A

S
|
S

E

A

R
|
R

S
|
S

R

A

G

G

K

K

R

S

L

L

K

K

Y

F

K

K

G

D

E

Q

D

D

I

I

E

T

V

I

Q

E

V

E

L
x
T

G

T

E

E

G

T

S

A

F

F

Q

E

G

G

V

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

G
x
S

G

S

T
|
T

S

S

L

A

E

K

W

Y

S

A

P

P

K

Y

A

A

A

V

Q

K

A

A

G

G

A

V

V

V

I

V

D

D

N
|
N

S
x
T

A
x
S

A

Y

W

F

R
|
R

M

Q

D

N

P

P

E

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

H

H

A

A

V

L

A

D

G

A

F

-

K

-

K

H

K

N

G

G

I

I

A

A

N

C

P
|
P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

I

V

H

R

D

Q

L

K

A

W

G

G

I

L

E

D

R

R

V

I

V

V

S

S

T

T

Y

Y

Q
|
Q

A

A

V

V

S

S

G
|
G

T

A

G
|
G

Q
x
M

K

G

A

A

I

I

V

L

E

E

L

T

Q

Q

G

R

Q

E

L

L

K

R

A

E

I

V

Y

L

C

N

Q

D

D

G

A

V

Q

K

P

P

-

C

-

D

-

L

Q

H

A

A

K

E

V

I

Y

L

P

P

-

S

-

G

-

D

-

K

-

H

H

Y

V

P

I

I

A

A

Q

F

N

N

V

A

L

L

P

P

H

Q

I

I

D

D

I

V

F

F

L

T

D

D

N

N

G

D

Y

Y

T

T

K

Y

E

E

M

M

K
|
K

M

M

V

T

H

K

E

E

T

T

V

K

K

K

I

I

M

M

E

E

D

D

P

D

T

S

I

I

K

A

V

V

S

S

A

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

I

L

Y

S

G

A

H
|
H

S

S

E

E

A

S

C

V

N

Y

I

I

T

E

T

T

A

K

R

E

K

V

I

A

S

P

A

I

Q

E

A

V

R

K

E

A

A

A

L

I

A

A

K

A

A

F

P

P

G

G

V

A

K

V

V

L

V

E

D

D

P

V

A

A

N

H

K

Q

L

I

Y

Y

P

P

M

Q

P

A

L

I

D

N

A

A

A

V

G

G

Q

S

D

R

L

D

T

T

L

F

V

V

G

G

R

R

I

I

R

R

E

K

D

D

V

L

S

D

Q

A

E

E

K

K

G

G

L

I

D

H

L

M

W

W

L

V

V

V

A

S

D

D

N

N

I

L

R

L

K

K

G
|
G

A

A

A

W

N

N

A

S

V

V

Q

Q

I

I

A

A

E

E

L

T

L

L

binding 3-[(1E)-3-carboxyprop-1-en-1-yl]benzene-1,2-dicarboxylic acid: S73 (≠ G76), T94 (≠ S97), S95 (≠ A98), R98 (= R101), N126 (= N131), C127 (= C132), Q154 (= Q159), G158 (= G163), K222 (= K217), R244 (= R239), H251 (= H246)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G11), T10 (= T13), G11 (= G14), A12 (= A15), V13 (= V16), A35 (= A38), S36 (= S39), R38 (= R41), S39 (= S42), T56 (≠ L59), S70 (= S73), A71 (= A74), G72 (= G75), T75 (= T78), N93 (= N96), T94 (≠ S97), P125 (= P130), N126 (= N131), C127 (= C132), G160 (= G165), M161 (≠ Q166), G328 (= G323)

4r4jA Crystal structure of complex sp_asadh with 3-carboxypropyl-phthalic acid and nicotinamide adenine dinucleotide phosphate (see paper)
52% identity, 97% coverage: 5:335/341 of query aligns to 2:340/359 of 4r4jA

query
sites
4r4jA

Y

Y

R

T

V

V

A

A

V

V

A

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

N

A

Q

Q

M

M

I

I

Q

K

C

M

L

L

E

E

R

S

D

T

F

L

P

P

I

I

G

D

E

K

L

I

R

R

L

Y

L

L

A
|
A

S
|
S

E

A

R
|
R

S
|
S

R

A

G

G

K

K

R

S

L

L

K

K

Y

F

K

K

G

D

E

Q

D

D

I

I

E

T

V

I

Q

E

V

E

L
x
T

G

T

E

E

G

T

S

A

F

F

Q

E

G

G

V

V

D

D

I

I

A

A

L

L

F

F

S
|
S

A
|
A

G
|
G

G

S

T
|
T

S

S

L

A

E

K

W

Y

S

A

P

P

K

Y

A

A

A

V

Q

K

A

A

G

G

A

V

V

V

I

V

D

D

N
|
N

S
x
T

A
x
S

A

Y

W

F

R
|
R

M

Q

D

N

P

P

E

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

H

H

A

A

V

L

A

D

G

A

F

-

K

-

K

H

K

N

G

G

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

V

V

V

A

L

L

K

E

P

P

I

V

H

R

D

Q

L

K

A

W

G

G

I

L

E

D

R

R

V

I

V

V

S

S

T

T

Y

Y

Q
|
Q

A

A

V

V

S

S

G
|
G

T

A

G
|
G

Q
x
M

K

G

A

A

I

I

V

L

E

E

L

T

Q

Q

G

R

Q

E

L

L

K

R

A

E

I

V