SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013258773.1 NCBI__GCF_000143965.1:WP_013258773.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1phpA Structure of the adp complex of the 3-phosphoglycerate kinase from bacillus stearothermophilus at 1.65 angstroms (see paper)
50% identity, 93% coverage: 9:391/413 of query aligns to 10:391/394 of 1phpA

query
sites
1phpA

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active site: R36 (= R36), K197 (= K198), G351 (= G351), G374 (= G374)
binding adenosine-5'-diphosphate: G195 (= G196), K201 (= K202), G219 (= G220), G220 (≠ A221), L237 (≠ M238), N316 (= N317), P318 (= P319), G320 (= G321), V321 (≠ A322), E323 (= E324), G350 (= G350), D352 (= D352), S353 (≠ T353)

P18912 Phosphoglycerate kinase; EC 2.7.2.3 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
50% identity, 93% coverage: 9:391/413 of query aligns to 10:391/394 of P18912

query
sites
P18912

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K201 (= K202) binding ATP
N316 (= N317) binding ATP
E323 (= E324) binding ATP
GGDS 350:353 (≠ GGDT 350:353) binding ATP

P36204 Bifunctional PGK/TIM; EC 2.7.2.3; EC 5.3.1.1 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
48% identity, 93% coverage: 9:391/413 of query aligns to 10:394/654 of P36204

query
sites
P36204

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R36 (= R36) binding substrate
R118 (= R118) binding substrate
R151 (= R151) binding substrate

1vpeA Crystallographic analysis of phosphoglycerate kinase from the hyperthermophilic bacterium thermotoga maritima (see paper)
47% identity, 93% coverage: 9:391/413 of query aligns to 9:393/398 of 1vpeA

query
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1vpeA

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V

V

G

G

P

D

E

E

V

V

E

K

K

K

M

A

V

V

S

E

Q

E

M

L

T

K

S

E

P

G

G

E

V

V

I

L

M

L

L

L

E

E

N

N

L

T

R

R

F

F

H

H

D

P

G

G

E

E

T

T

K

K

N

N

D

D

P

P

E

E

F

L

S

A

Q

K

A

F

L

W

A

A

R

S

L

L

C

A

D

D

V

I

Y

H

V

V

D

N

D

D

A

A

F

F

A

G

V

T

A

A

H

H

R

R

A

A

H

H

A

A

S

S

V

N

V

V

G

G

V

I

T

A

Q

Q

Y

F

A

I

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P

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-

S

S

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V

A

A

G

G

F

F

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L

M

M

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K

K

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L

I

D

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Y

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F

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N

P

P

A

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K

P

P

L

Y

A

V

A

V

V

V

I

L

G

G

G
|
G

A
|
A

K
|
K

A

V

V

S

T

D

K
|
K

L

I

E

G

A

V

L

I

E

T

N

N

L

L

L

M

N

E

H

K

V

A

D

D

K

R

I

I

I

L

V

I

G

G

G
|
G

A
|
A

M

M

A

M

N

F

T

T

F

F

L

L

K

K

G

A

V

L

D

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V

G

G

N

S

S

S

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D

L

K

V

I

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V

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A

A

N

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C

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L

L

L

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R

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K

K

A

A

K

K

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E

L

K

G

G

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V

K

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I

Y

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I

L

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P

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V

D

D

C

A

V

V

V

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A

A

D

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P

P

K

G

A

V

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K

M

K

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T

V

T

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V

I

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D

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D

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G

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N

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D

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W

W

M

M

I

G

L

L

D

D

I

I

G

G

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P

A

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T

T

S

I

M

E

L

L

Y

F

R

K

E

Q

A

K

L

L

A

S

N

D

C

A

K

K

T

T

V

V

I

V

W

W

N
|
N

G

G

P
|
P

M

M

G
|
G

A
x
V

F

F

E
|
E

M

I

D

D

A

D

F

F

S

A

R

E

G

G

T

T

Y

K

N

Q

M

V

-

A

-

L

-

A

V

I

S

A

T

A

V

L

A

T

Q

E

S

K

Y

G

A

A

L

I

S

T

I

V

V

V

G

G

G
|
G

G
|
G

D
|
D

T
x
S

D

A

V

A

A

A

I

V

H

N

R

K

L

F

G

G

E

L

T

E

D

D

N

K

I

F

S

S

Y

H

I

V

S

S

T

T

G

G

G

G

G
|
G

A

A

F

S

L

L

A

E

M

F

L

L

T

E

G

G

D

K

V

E

L

L

P

P

A

G

V

I

E

A

A

S

L

M

active site: R35 (= R36), K196 (= K198), G353 (= G351), G376 (= G374)
binding phosphoaminophosphonic acid-adenylate ester: G194 (= G196), A195 (= A197), K196 (= K198), K200 (= K202), G218 (= G220), A219 (= A221), N316 (= N317), P318 (= P319), G320 (= G321), V321 (≠ A322), E323 (= E324), G352 (= G350), G353 (= G351), D354 (= D352), S355 (≠ T353)

P40924 Phosphoglycerate kinase; EC 2.7.2.3 from Bacillus subtilis (strain 168) (see paper)
48% identity, 93% coverage: 9:391/413 of query aligns to 10:391/394 of P40924

query
sites
P40924

D

D

L

V

K

K

D

G

K

K

R

V

V

V

L

F

I

C

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

M

D

-

E

K

E

D

R

G

N

E

I

V

A

T

D

D

D

D

N

T

R

R

I

I

R

R

A

A

A

A

L

L

P

P

T

T

I

I

N

K

Y

H

V

L

L

A

D

D

E

Q

G

G

G

A

K

K

V

V

I

L

V

L

A

A

S

S

H

H

M

L

G

G

R

R

P

P

K

K

G

G

K

E

R

V

V

V

E

E

S

E

L

L

S

R

M

L

A

T

P

P

V

V

A

A

R

A

R

R

L

L

G

G

R

E

L

L

L

L

K

G

K

K

E

E

V

V

A

K

L

K

A

A

P

D

D

E

C

A

V

Y

G

G

P

D

E

A

V

V

E

K

K

A

M

Q

V

I

S

S

Q

E

M

M

T

K

S

D

P

G

G

D

V

V

I

L

M

V

L

L

E

E

N

N

L

V

R

R

F

F

H

Y

D

P

G

G

E

E

T

E

K

K

N

N

D

D

P

P

E

E

F

L

S

A

Q

K

A

A

L

F

A

A

R

E

L

L

C

A

D

D

V

V

Y

Y

V

V

D

N

D

D

A

A

F

F

A

G

V

A

A

A

H

H

R

R

A

A

H

H

A

A

S

S

V

T

V

A

G

G

V

I

T

A

Q

E

Y

H

A

L

P

P

V

-

S

A

V

V

A

A

G

G

F

F

T

L

M

M

K

E

K

K

E

E

L

L

D

D

Y

V

F

L

R

G

R

K

A

A

M

V

I
x
S

D

N

P

P

A

D

R

R

P

P

L

F

A

T

A

A

V

I

I

I

G

G

G

G

A

A

K

K

A

V

V

K

T

D

K

K

L

I

E

G

A

V

L

I

E

E

N

S

L

L

L

L

N

D

H

K

V

V

D

D

K

N

I

L

I

I

V

I

G

G

G

G

A

G

M

L

A

A

N

Y

T

T

F

F

L

V

K

K

G

A

V

L

D

G

Y

Y

S

E

V

V

G

G

N

K

S

S

M

L

F

L

E

E

P

E

E

D

L

K

V

I

P

E

V

L

A

A

N

K

C

S

L

F

L

M

R

D

K

R

A

A

K

K

E

E

L

K

G

G

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V

K

N

L

F

Y

Y

I

M

P

P

V

E

D

D

C

V

V

L

V

V

A

A

D

D

R

D

F

F

D

S

P

N

K

D

A

A

E

N

T

V

M

K

I

I

T

V

T

P

V

I

Q

S

E

E

V

I

P

P

N

S

D

D

W

L

M

E

I

A

L

I

D

D

I

I

G

G

P

T

A

K

T
|
T

S

R

M

E

L

T

Y

Y

R

A

E

D

A

V

L

I

A

K

N

N

C

S

K

K

T

L

V

V

I

V

W

W

N

N

G

G

P

P

M

M

G

G

A

V

F

F

E

E

M

I

D

D

A

L

F

F

S

A

R

Q

G

G

T

T

Y

K

N

A

M

V

V

A

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T

A

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L

A

A

Q

E

S

A

Y

K

-

D

A

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L

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S

S

I

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V

I

G

G

G

G

G

G

D

D

T

S

D

A

V

A

A

A

I

V

H

E

R

K

L

F

G

G

E

L

T

A

D

D

N

K

I

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S

S

Y

H

I

I

S

S

T

T

G

G

G

G

G

G

A

A

F

S

L

L

A

E

M

F

L

M

T

E

G

G

D

K

V

E

L

L

P

P

A

G

V

V

E

A

A

A

L

L

S183 (≠ I184) modified: Phosphoserine
T299 (= T300) modified: Phosphothreonine

4feyA An x-ray structure of a putative phosphogylcerate kinase with bound adp from francisella tularensis subsp. Tularensis schu s4
47% identity, 93% coverage: 9:391/413 of query aligns to 10:386/392 of 4feyA

query
sites
4feyA

D

D

L

L

K

K

D

D

K

K

R

K

V

V

L

L

I

V

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

V

D

K

E

D

E

G

R

K

N

-

I

V

A

T

D

S

D

K

N

V

R
|
R

I

I

R

E

A

A

A

A

L

I

P

P

T

T

I

I

N

Q

Y

Y

V

I

L

L

D

D

E

Q

G

G

G

G

K

A

V

V

I

I

V

L

A

M

S

S

H

H

M

L

G

G

R

R

P

P

-

T

K

E

G

G

K

E

R

Y

V

D

E

S

S

Q

L

F

S

S

M

L

A

E

P

P

V

V

A

A

R

K

R

A

L

L

G

S

R

E

L

I

L

I

K

N

K

K

E

P

V

V

A

K

L

F

A

A

P

K

D

D

C

W

V

L

-

D

G

G

P

V

E

D

V

V

E

K

K

-

M

-

V

-

S

-

Q

-

M

-

T

-

S

A

P

G

G

E

V

I

I

V

M

M

L

C

E

E

N

N

L

V

R

R

F

F

H

N

D

S

G

G

E

E

T

K

K

K

N

S

D

T

P

D

E

D

F

L

S

S

Q

K

A

K

L

I

A

A

R

S

L

L

C

G

D

D

V

V

Y

F

V

V

D

M

D

D

A

A

F

F

A

A

V

T

A

A

H

H

R

R

A

A

H

Q

A

A

S

S

V

T

V

Y

G

G

V

V

T

A

Q

K

Y

Y

A

I

P

P

V

V

S

A

V

C

A

A

G

G

F

I

T

L

M

L

K

T

K

N

E

E

L

I

D

Q

Y

A

F

L

R

E

R

K

A

A

M

L

I

K

D

S

P

P

A

K

R

K

P

P

L

M

A

A

A

A

V

I

I

V

G

G

G
|
G

A
x
S

K
|
K

A

V

V

S

T

T

K
|
K

L

L

E

S

A

V

L

L

E

N

N

N

L

L

L

L

N

D

H

K

V

V

D

E

K

I

I

L

I

I

V

V

G

G

G
|
G

A

G

M

I

A

A

N

N

T

T

F

F

L

I

K

K

G

A

V

E

D

G

Y

F

S

D

V

V

G

G

N

N

S

S

M

L

F

Y

E

E

P

Q

E

D

L

L

V

V

P

A

V

E

A

A

N

T

C

E

L

I

L

L

R

A

K

K

A

A

K

K

E

A

L

L

G

G

V

V

K

N

L

I

Y

P

I

V

P

P

V

V

D

D

C

V

V

R

V

V

A

A

D

K

R

E

F

F

D

S

P

E

K

N

A

A

E

Q

T

A

M

I

I

I

T

K

T

K

V

V

Q

S

E

D

V

V

P

V

N

A

D

D

W

E

M

M

I

I

L

L

D

D

I

I

G

G

P

P

A

E

T

S

S

Q

M

K

L

I

Y

I

R

A

E

E

A

L

L

L

A

K

N

S

C

A

K

N

T

T

V

I

I

L

W

W

N

N

G

G

P

P

M

V

G
|
G

A
x
V

F

F

E
|
E

M

F

D

D

A

N

F

F

S

A

R

E

G

G

T

T

Y

K

N

A

M

L

V

S

S

L

T

A

V

I

A

A

Q

Q

S

S

Y

H

A

A

L

F

S

S

I

V

V

A

G

G

G

G

G
|
G

D
|
D

T

T

D

I

V

A

A

A

I

I

H

E

R

K

L

F

G

G

E

I

T

K

D

D

N

Q

I

V

S

S

Y

Y

I

I

S

S

T

T

G

A

G

G

G
|
G

A

A

F

F

L

L

A

E

M

F

L

L

T

E

G

G

D

K

V

K

L

L

P

P

A

A

V

I

E

E

A

I

L

L

active site: R36 (= R36), K193 (= K198), G346 (= G351), G369 (= G374)
binding adenosine-5'-diphosphate: G191 (= G196), S192 (≠ A197), K197 (= K202), G215 (= G220), G316 (= G321), V317 (≠ A322), E319 (= E324), D347 (= D352)

4axxA The catalytically active fully closed conformation of human phosphoglycerate kinase in complex with adp 3-phosphoglycerate and beryllium trifluoride
46% identity, 93% coverage: 9:391/413 of query aligns to 11:404/407 of 4axxA

query
sites
4axxA

D

D

L

V

K

K

D

G

K

K

R

R

V

V

L

V

I

M

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

M

D

-

E

K

E

N

R

N

N

Q

I

I

A

T

D

N

D

N

N

Q

R
|
R

I

I

R

K

A

A

A

A

L

V

P

P

T

S

I

I

N

K

Y

F

V

C

L

L

D

D

E

N

G

G

G

A

K

K

-

S

V

V

I

V

V

L

A

M

S

S

H

H

M

L

G

G

R

R

P

P

K

D

G

G

K

V

R

P

V

M

-

P

E

D

S

K

L

Y

S

S

M

L

A

E

P

P

V

V

A

A

R

V

R

E

L

L

G

K

R

S

L

L

L

L

K

G

K

K

E

D

V

V

A

L

L

F

A

L

P

K

D

D

C

C

V

V

G

G

P

P

E

E

V

V

E

E

K

K

M

A

V

C

S

A

Q

N

M

P

T

A

S

A

P

G

G

S

V

V

I

I

M

L

L

L

E

E

N

N

L

L

R

R

F

F

H

H

-

V

-

E

-

E

D

E

G

G

E

K

T

A

K

K

N

A

D

E

P

P

E

A

-

K

-

I

-

E

-

A

F

F

S

R

Q

A

A

S

L

L

A

S

R

K

L

L

C

G

D

D

V

V

Y

Y

V

V

D

N

D

D

A

A

F

F

A

G

V

T

A

A

H

H

R

R

A

A

H

H

A

S

S

S

V

M

V

V

G

G

V

V

T

N

Q

-

Y

-

A

L

P

P

V

Q

S

K

V

A

A

G

G

G

F

F

T

L

M

M

K

K

K

K

E

E

L

L

D

N

Y

Y

F

F

R

A

R

K

A

A

M

L

I

E

D

S

P

P

A

E

R

R

P

P

L

F

A

L

A

A

V

I

I

L

G

G

G
|
G

A
|
A

K
|
K

A

V

V

A

T

D

K
|
K

L

I

E

Q

A

L

L

I

E

N

N

N

L

M

L

L

N

D

H

K

V

V

D

N

K

E

I

M

I

I

V

I

G

G

G
|
G

A
x
G

M

M

A

A

N

F

T

T

F

F

L

L

K

K

G

V

V

L

-

N

D

N

Y

M

S

E

V

I

G

G

N

T

S

S

M

L

F

F

E

D

P

E

E

E

L

G

V

A

P

K

V

I

A

V

N

K

C

D

L

L

L

M

R

S

K

K

A

A

K

E

E

K

L

N

G

G

V

V

K

K

L

I

Y

T

I

L

P

P

V

V

D

D

C

F

V

V

V

T

A

A

D

D

R

K

F

F

D

D

P

E

K

N

A

A

E

K

T

T

M

G

I

Q

T

A

T

T

V

V

Q

A

E

S

-

G

V

I

P

P

N

A

D

G

W

W

M

M

I

G

L

L

D

D

I

C

G

G

P

P

A

E

T

S

S

S

M

K

L

K

Y

Y

R

A

E

E

A

A

L

V

A

T

N

R

C

A

K

K

T

Q

V

I

I

V

W

W

N
|
N

G

G

P
|
P

M

V

G
|
G

A
x
V

F

F

E
|
E

M

W

D

E

A

A

F

F

S

A

R

R

G

G

T

T

Y

K

N

A

M

L

V

M

S

D

T

E

V

V

A

V

Q

K

S

A

Y

T

A

S

-

R

-

G

-

C

L

I

S

T

I

I

V

I

G

G

G
|
G

G
|
G

D
|
D

T
|
T

D

A

V

T

A

C

I

C

H

A

R

K

L

W

G

N

E

T

T

E

D

D

N

K

I

V

S

S

Y

H

I

V

S

S

T

T

G

G

G

G

G
|
G

A

A

F

S

L

L

A

E

M

L

L

L

T

E

G

G

D

K

V

V

L

L

P

P

A

G

V

V

E

D

A

A

L

L

active site: R37 (= R36), K206 (= K198), G364 (= G351), G387 (= G374)
binding adenosine-5'-diphosphate: G204 (= G196), A205 (= A197), K210 (= K202), G228 (= G220), G229 (≠ A221), N327 (= N317), P329 (= P319), G331 (= G321), V332 (≠ A322), E334 (= E324), G363 (= G350), G364 (= G351), D365 (= D352), T366 (= T353)
binding beryllium trifluoride ion: K206 (= K198), K210 (= K202), G363 (= G350)

2wzcA The catalytically active fully closed conformation of human phosphoglycerate kinase in complex with adp, 3pg and aluminium tetrafluoride (see paper)
46% identity, 93% coverage: 9:391/413 of query aligns to 11:402/405 of 2wzcA

query
sites
2wzcA

D

D

L

V

K

K

D

G

K

K

R

R

V

V

L

V

I

M

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

M

D

-

E

K

E

N

R

N

N

Q

I

I

A

T

D

N

D

N

N

Q

R
|
R

I

I

R

K

A

A

A

A

L

V

P

P

T

S

I

I

N

K

Y

F

V

C

L

L

D

D

E

N

G

G

G

A

K

K

-

S

V

V

I

V

V

L

A

M

S

S

H

H

M

L

G

G

R

R

P

P

K

D

G

G

K

V

R

P

V

M

-

P

E

D

S

K

L

Y

S

S

M

L

A

E

P

P

V

V

A

A

R

V

R

E

L

L

G

K

R

S

L

L

L

L

K

G

K

K

E

D

V

V

A

L

L

F

A

L

P

K

D

D

C

C

V

V

G

G

P

P

E

E

V

V

E

E

K

K

M

A

V

C

S

A

Q

N

M

P

T

A

S

A

P

G

G

S

V

V

I

I

M

L

L

L

E

E

N

N

L

L

R

R

F

F

H

H

-

V

D

E

G

E

E

E

T

G

K

K

N

G

D

E

P

P

E

A

-

K

-

I

-

E

-

A

F

F

S

R

Q

A

A

S

L

L

A

S

R

K

L

L

C

G

D

D

V

V

Y

Y

V

V

D

N

D

D

A

A

F

F

A

G

V

T

A

A

H

H

R

R

A

A

H

H

A

S

S

S

V

M

V

V

G

G

V

V

T

N

Q

-

Y

-

A

L

P

P

V

Q

S

K

V

A

A

G

G

G

F

F

T

L

M

M

K

K

K

K

E

E

L

L

D

N

Y

Y

F

F

R

A

R

K

A

A

M

L

I

E

D

S

P

P

A

E

R

R

P

P

L

F

A

L

A

A

V

I

I

L

G

G

G
|
G

A
|
A

K
|
K

A

V

V

A

T

D

K
|
K

L

I

E

Q

A

L

L

I

E

N

N

N

L

M

L

L

N

D

H

K

V

V

D

N

K

E

I

M

I

I

V

I

G

G

G
|
G

A
x
G

M

M

A

A

N

F

T

T

F

F

L

L

K

K

G

V

V

L

-

N

D

N

Y

M

S

E

V

I

G

G

N

T

S

S

M

L

F

F

E

D

P

E

E

E

L

G

V

A

P

K

V

I

A

V

N

K

C

D

L

L

L

M

R

S

K

K

A

A

K

E

E

K

L

N

G

G

V

V

K

K

L

I

Y

T

I

L

P

P

V

V

D

D

C

F

V

V

V

T

A

A

D

D

R

K

F

F

D

D

P

E

K

N

A

A

E

K

T

T

M

G

I

Q

T

A

T

T

V

V

Q

A

E

S

-

G

V

I

P

P

N

A

D

G

W

W

M

M

I

G

L

L

D

D

I

C

G

G

P

P

A

E

T

S

S

S

M

K

L

K

Y

Y

R

A

E

E

A

A

L

V

A

T

N

R

C

A

K

K

T

Q

V

I

I

V

W

W

N
|
N

G

G

P
|
P

M

V

G
|
G

A
x
V

F

F

E
|
E

M

W

D

E

A

A

F

F

S

A

R

R

G

G

T

T

Y

K

N

A

M

L

V

M

S

D

T

E

V

V

A

V

Q

K

S

A

Y

T

A

S

-

R

-

G

-

C

L

I

S

T

I

I

V

I

G

G

G
|
G

G
|
G

D
|
D

T
|
T

D

A

V

T

A

C

I

C

H

A

R

K

L

W

G

N

E

T

T

E

D

D

N

K

I

V

S

S

Y

H

I

V

S

S

T

T

G

G

G
|
G

G
|
G

A

A

F

S

L

L

A

E

M

L

L

L

T

E

G

G

D

K

V

V

L

L

P

P

A

G

V

V

E

D

A

A

L

L

active site: R37 (= R36), K204 (= K198), G362 (= G351), G385 (= G374)
binding adenosine-5'-diphosphate: G202 (= G196), A203 (= A197), K204 (= K198), K208 (= K202), G226 (= G220), G227 (≠ A221), N325 (= N317), P327 (= P319), G329 (= G321), V330 (≠ A322), E332 (= E324), G361 (= G350), D363 (= D352), T364 (= T353)
binding tetrafluoroaluminate ion: R37 (= R36), K204 (= K198), K208 (= K202), G361 (= G350), G362 (= G351), G384 (= G373)

2wzbA The catalytically active fully closed conformation of human phosphoglycerate kinase in complex with adp, 3pg and magnesium trifluoride (see paper)
46% identity, 93% coverage: 9:391/413 of query aligns to 11:402/405 of 2wzbA

query
sites
2wzbA

D

D

L

V

K

K

D

G

K

K

R

R

V

V

L

V

I

M

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

M

D

-

E

K

E

N

R

N

N

Q

I

I

A

T

D

N

D

N

N

Q

R
|
R

I

I

R

K

A

A

A

A

L

V

P

P

T

S

I

I

N

K

Y

F

V

C

L

L

D

D

E

N

G

G

G

A

K

K

-

S

V

V

I

V

V

L

A

M

S

S

H

H

M

L

G

G

R

R

P

P

K

D

G

G

K

V

R

P

V

M

-

P

E

D

S

K

L

Y

S

S

M

L

A

E

P

P

V

V

A

A

R

V

R

E

L

L

G

K

R

S

L

L

L

L

K

G

K

K

E

D

V

V

A

L

L

F

A

L

P

K

D

D

C

C

V

V

G

G

P

P

E

E

V

V

E

E

K

K

M

A

V

C

S

A

Q

N

M

P

T

A

S

A

P

G

G

S

V

V

I

I

M

L

L

L

E

E

N

N

L

L

R

R

F

F

H

H

-

V

D

E

G

E

E

E

T

G

K

K

N

G

D

E

P

P

E

A

-

K

-

I

-

E

-

A

F

F

S

R

Q

A

A

S

L

L

A

S

R

K

L

L

C

G

D

D

V

V

Y

Y

V

V

D

N

D

D

A

A

F

F

A

G

V

T

A

A

H

H

R

R

A

A

H

H

A

S

S

S

V

M

V

V

G

G

V

V

T

N

Q

-

Y

-

A

L

P

P

V

Q

S

K

V

A

A

G

G

G

F

F

T

L

M

M

K

K

K

K

E

E

L

L

D

N

Y

Y

F

F

R

A

R

K

A

A

M

L

I

E

D

S

P

P

A

E

R

R

P

P

L

F

A

L

A

A

V

I

I

L

G

G

G
|
G

A
|
A

K
|
K

A

V

V

A

T

D

K
|
K

L

I

E

Q

A

L

L

I

E

N

N

N

L

M

L

L

N

D

H

K

V

V

D

N

K

E

I

M

I

I

V

I

G

G

G
|
G

A
x
G

M

M

A

A

N

F

T

T

F

F

L

L

K

K

G

V

V

L

-

N

D

N

Y

M

S

E

V

I

G

G

N

T

S

S

M

L

F

F

E

D

P

E

E

E

L

G

V

A

P

K

V

I

A

V

N

K

C

D

L

L

L

M

R

S

K

K

A

A

K

E

E

K

L

N

G

G

V

V

K

K

L

I

Y

T

I

L

P

P

V

V

D

D

C

F

V

V

V

T

A

A

D

D

R

K

F

F

D

D

P

E

K

N

A

A

E

K

T

T

M

G

I

Q

T

A

T

T

V

V

Q

A

E

S

-

G

V

I

P

P

N

A

D

G

W

W

M

M

I

G

L

L

D

D

I

C

G

G

P

P

A

E

T

S

S

S

M

K

L

K

Y

Y

R

A

E

E

A

A

L

V

A

T

N

R

C

A

K

K

T

Q

V

I

I

V

W

W

N
|
N

G

G

P
|
P

M

V

G
|
G

A
x
V

F

F

E
|
E

M

W

D

E

A

A

F

F

S

A

R

R

G

G

T

T

Y

K

N

A

M

L

V

M

S

D

T

E

V

V

A

V

Q

K

S

A

Y

T

A

S

-

R

-

G

-

C

L

I

S

T

I

I

V

I

G

G

G
|
G

G
|
G

D
|
D

T
|
T

D

A

V

T

A

C

I

C

H

A

R

K

L

W

G

N

E

T

T

E

D

D

N

K

I

V

S

S

Y

H

I

V

S

S

T

T

G

G

G
|
G

G
|
G

A

A

F

S

L

L

A

E

M

L

L

L

T

E

G

G

D

K

V

V

L

L

P

P

A

G

V

V

E

D

A

A

L

L

active site: R37 (= R36), K204 (= K198), G362 (= G351), G385 (= G374)
binding adenosine-5'-diphosphate: G202 (= G196), A203 (= A197), K204 (= K198), K208 (= K202), G226 (= G220), G227 (≠ A221), N325 (= N317), P327 (= P319), G329 (= G321), V330 (≠ A322), E332 (= E324), G361 (= G350), D363 (= D352), T364 (= T353)
binding trifluoromagnesate: K204 (= K198), K208 (= K202), G361 (= G350), G384 (= G373), G385 (= G374)

1ltkC Crystal structure of phosphoglycerate kinase from plasmodium falciparum, in the open conformation
45% identity, 94% coverage: 4:391/413 of query aligns to 16:421/424 of 1ltkC

query
sites
1ltkC

I

I

N

S

Q

D

L

L

E

K

D

D

L

I

K

K

D

N

K

K

R

K

V

V

L

L

I

V

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

I

D

-

E

E

E

N

R

G

N

I

I

I

A

K

D

D

D

T

N

N

R
|
R

I

I

R

T

A

A

A

T

L

L

P

P

T

T

I

I

N

N

Y

H

V

L

L

K

D

K

E

E

G

G

G

A

-

S

K

K

V

I

I

I

V

L

A

I

S

S

H

H

M

C

G

G

R

R

P

P

K

D

G

G

K

L

R

R

V

N

E

E

S

K

L

Y

S

T

M

L

A

K

P

P

V

V

A

A

R

E

R

T

L

L

G

K

R

G

L

L

L

L

K

G

K

E

E

E

V

V

A

L

L

F

A

L

P

N

D

D

C

C

V

V

G

G

P

K

E

E

V

V

E

E

K

D

M

K

V

I

S

N

Q

A

M

A

T

K

S

E

P

N

G

S

V

V

I

I

M

L

L

L

E

E

N

N

L

L

R

R

F

F

H

H

D

I

G

E

E

E

-

E

-

G

-

K

-

G

-

V

-

D

-

A

-

N

-

G

-

N

-

K

-

V

-

K

-

A

T

N

K

K

N

E

D

D

P

V

E

E

-

K

F

F

S

Q

Q

N

A

D

L

L

A

T

R

K

L

L

C

A

D

D

V

V

Y

F

V

I

D

N

D

D

A

A

F

F

A

G

V

T

A

A

H

H

R

R

A

A

H

H

A

S

S

S

V

M

V

V

G

G

V

V

T

K

Q

-

Y

-

A

L

P

N

V

V

S

K

V

A

A

S

G

G

F

F

T

L

M

M

K

K

K

K

E

E

L

L

D

E

Y

Y

F

F

R

S

R

K

A

A

M

L

I

E

D

N

P

P

A

Q

R

R

P

P

L

L

A

L

A

A

V

I

I

L

G

G

G
|
G

A
|
A

K
|
K

A

V

V

S

T

D

K

K

L

I

E

Q

A

L

L

I

E

K

N

N

L

L

L

L

N

D

H

K

V

V

D

D

K

R

I

M

I

I

V

I

G

G

G
|
G

A
x
G

M

M

A

A

N

Y

T

T

F

F

L

K

K

K

G

V

V

L

-

N

D

N

Y

M

S

K

V

I

G

G

N

T

S

S

M

L

F

F

E

D

P

E

E

A

L

G

V

S

P

K

V

I

A

V

N

G

C

E

L

I

L

M

R

E

K

K

A

A

K

K

E

A

L

K

G

N

V

V

K

Q

L

I

Y

F

I

L

P

P

V

V

D

D

C

F

V

K

V

I

A

A

D

D

R

N

F

F

D

D

P

N

K

N

A

A

E

N

T

T

M

K

I

F

T

V

T

T

V

D

Q

E

E

E

-

G

V

I

P

P

N

D

D

N

W

W

M

M

I

G

L

L

D

D

I

A

G

G

P

P

A

K

T

S

S

I

M

E

L

N

Y

Y

R

K

E

D

A

V

L

I

A

L

N

T

C

S

K

K

T

T

V

V

I

I

W

W

N

N

G

G

P

P

M

Q

G
|
G

A
x
V

F

F

E
|
E

M

M

D

P

A

N

F

F

S

A

R

K

G

G

T

S

-

I

-

E

-

C

Y

L

N

N

M

L

V

V

S

V

T

E

V

V

A

T

Q

K

S

K

Y

G

A

A

L

I

S

T

I

I

V

V

G

G

G

G

G
|
G

D
|
D

T

T

D

A

V

S

A

L

I

V

H

E

R

Q

L

Q

G

N

E

K

T

K

D

N

N

E

I

I

S

S

Y

H

I

V

S

S

T

T

G

G

G

G

G
|
G

A

A

F

S

L

L

A

E

M

L

L

L

T

E

G

G

D

K

V

E

L

L

P

P

A

G

V

V

E

L

A

A

L

L

active site: R47 (= R36), K223 (= K198), G381 (= G351), G404 (= G374)
binding adenosine monophosphate: G221 (= G196), A222 (= A197), K223 (= K198), G245 (= G220), G246 (≠ A221), G348 (= G321), V349 (≠ A322), E351 (= E324), D382 (= D352)

2wzdA The catalytically active fully closed conformation of human phosphoglycerate kinase k219a mutant in complex with adp, 3pg and aluminium trifluoride (see paper)
46% identity, 93% coverage: 9:391/413 of query aligns to 11:402/405 of 2wzdA

query
sites
2wzdA

D

D

L

V

K

K

D

G

K

K

R

R

V

V

L

V

I

M

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

M

D

-

E

K

E

N

R

N

N

Q

I

I

A

T

D

N

D

N

N

Q

R
|
R

I

I

R

K

A

A

A

A

L

V

P

P

T

S

I

I

N

K

Y

F

V

C

L

L

D

D

E

N

G

G

G

A

K

K

-

S

V

V

I

V

V

L

A

M

S

S

H

H

M

L

G

G

R

R

P

P

K

D

G

G

K

V

R

P

V

M

-

P

E

D

S

K

L

Y

S

S

M

L

A

E

P

P

V

V

A

A

R

V

R

E

L

L

G

K

R

S

L

L

L

L

K

G

K

K

E

D

V

V

A

L

L

F

A

L

P

K

D

D

C

C

V

V

G

G

P

P

E

E

V

V

E

E

K

K

M

A

V

C

S

A

Q

N

M

P

T

A

S

A

P

G

G

S

V

V

I

I

M

L

L

L

E

E

N

N

L

L

R

R

F

F

H

H

-

V

D

E

G

E

E

E

T

G

K

K

N

G

D

E

P

P

E

A

-

K

-

I

-

E

-

A

F

F

S

R

Q

A

A

S

L

L

A

S

R

K

L

L

C

G

D

D

V

V

Y

Y

V

V

D

N

D

D

A

A

F

F

A

G

V

T

A

A

H

H

R

R

A

A

H

H

A

S

S

S

V

M

V

V

G

G

V

V

T

N

Q

-

Y

-

A

L

P

P

V

Q

S

K

V

A

A

G

G

G

F

F

T

L

M

M

K

K

K

K

E

E

L

L

D

N

Y

Y

F

F

R

A

R

K

A

A

M

L

I

E

D

S

P

P

A

E

R

R

P

P

L

F

A

L

A

A

V

I

I

L

G

G

G
|
G

A
|
A

K
|
K

A

V

V

A

T

D

K

A

L

I

E

Q

A

L

L

I

E

N

N

N

L

M

L

L

N

D

H

K

V

V

D

N

K

E

I

M

I

I

V

I

G

G

G
|
G

A
x
G

M

M

A

A

N

F

T

T

F

F

L

L

K

K

G

V

V

L

-

N

D

N

Y

M

S

E

V

I

G

G

N

T

S

S

M

L

F

F

E

D

P

E

E

E

L

G

V

A

P

K

V

I

A

V

N

K

C

D

L

L

L

M

R

S

K

K

A

A

K

E

E

K

L

N

G

G

V

V

K

K

L

I

Y

T

I

L

P

P

V

V

D

D

C

F

V

V

V

T

A

A

D

D

R

K

F

F

D

D

P

E

K

N

A

A

E

K

T

T

M

G

I

Q

T

A

T

T

V

V

Q

A

E

S

-

G

V

I

P

P

N

A

D

G

W

W

M

M

I

G

L

L

D

D

I

C

G

G

P

P

A

E

T

S

S

S

M

K

L

K

Y

Y

R

A

E

E

A

A

L

V

A

T

N

R

C

A

K

K

T

Q

V

I

I

V

W

W

N
|
N

G

G

P
|
P

M

V

G
|
G

A
x
V

F

F

E
|
E

M

W

D

E

A

A

F

F

S

A

R

R

G

G

T

T

Y

K

N

A

M

L

V

M

S

D

T

E

V

V

A

V

Q

K

S

A

Y

T

A

S

-

R

-

G

-

C

L

I

S

T

I

I

V

I

G

G

G
|
G

G
|
G

D
|
D

T
|
T

D

A

V

T

A

C

I

C

H

A

R

K

L

W

G

N

E

T

T

E

D

D

N

K

I

V

S

S

Y

H

I

V

S

S

T

T

G

G

G
|
G

G
|
G

A

A

F

S

L

L

A

E

M

L

L

L

T

E

G

G

D

K

V

V

L

L

P

P

A

G

V

V

E

D

A

A

L

L

active site: R37 (= R36), K204 (= K198), G362 (= G351), G385 (= G374)
binding adenosine-5'-diphosphate: G202 (= G196), A203 (= A197), K204 (= K198), G226 (= G220), G227 (≠ A221), N325 (= N317), P327 (= P319), G329 (= G321), V330 (≠ A322), E332 (= E324), G361 (= G350), D363 (= D352), T364 (= T353)
binding aluminum fluoride: R37 (= R36), K204 (= K198), G361 (= G350), G362 (= G351), G384 (= G373)

O60101 Phosphoglycerate kinase; EC 2.7.2.3 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
46% identity, 93% coverage: 9:391/413 of query aligns to 13:411/414 of O60101

query
sites
O60101

D

D

L

L

K

K

D

G

K

K

R

N

V

V

L

L

I

I

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

L

D

D

E

G

E

D

R

R

N

-

I

I

A

T

D

N

D

N

N

A

R

R

I

I

R

V

A

G

A

A

L

L

P

P

T

T

I

I

N

K

Y

Y

V

A

L

L

D

E

E

Q

G

Q

G

P

K

K

-

A

V

V

I

I

V

L

A

M

S

S

H

H

M

L

G

G

R

R

P

P

K

N

G

G

K

A

R

R

V

V

E

A

S

K

L
x
Y

S
|
S

M

L

A

K

P

P

V

V

A

A

R

A

R

E

L

L

G

S

R

K

L

L

L

L

K

G

K

K

E

P

V

V

A

K

L

F

A

L

P

D

D

D

C

C

V

V

G

G

P

P

E

E

V

V

E

E

K

K

M

A

V

C

S

K

Q

E

M

A

T

K

S

G

P

G

G

E

V

V

I

I

M

L

L

L

E

E

N

N

L

L

R

R

F

F

H

H

-

I

-

E

-

E

D

E

G

G

E

S

T

A

K

K

N

V

D

D

P

G

E

K

-

K

-

V

-

K

-

A

-

D

-

A

-
x
S

-

A

-

V

-

E

-

A

F

F

S

R

Q

K

A

S

L

L

A

T

R

S

L

L

C

G

D

D

V

I

Y

F

V

V

D

N

D

D

A

A

F

F

A

G

V

T

A

A

H

H

R

R

A

A

H

H

A
x
S

S
|
S

V

M

V

V

G

G

V

V

T

D

Q

L

Y

P

A

-

P

-