SitesBLAST
Comparing WP_013289899.1 NCBI__GCF_000166355.1:WP_013289899.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
A8B2U2 Fructose-bisphosphate aldolase; Glfba; glFBPA; Fructose-1,6-bisphosphate aldolase; EC 4.1.2.13 from Giardia intestinalis (strain ATCC 50803 / WB clone C6) (Giardia lamblia) (see 4 papers)
55% identity, 96% coverage: 1:311/323 of query aligns to 1:307/323 of A8B2U2
- S50 (= S50) binding beta-D-fructose 1,6-bisphosphate
- D83 (= D83) active site, Proton donor; mutation to A: Severe loss of catalytic activity.
- H84 (= H84) binding Zn(2+)
- H178 (= H181) binding beta-D-fructose 1,6-bisphosphate; binding Zn(2+)
- G179 (= G182) binding beta-D-fructose 1,6-bisphosphate
- K182 (= K185) binding beta-D-fructose 1,6-bisphosphate
- H210 (= H213) binding Zn(2+)
- G211 (= G214) binding beta-D-fructose 1,6-bisphosphate
- S213 (= S216) binding beta-D-fructose 1,6-bisphosphate
- N253 (= N256) binding beta-D-fructose 1,6-bisphosphate
- D255 (= D258) binding beta-D-fructose 1,6-bisphosphate; mutation to A: 9.4-fold reduction in substrate affinity and 50-fold reduction in catalytic affinity. Has some activity towards tagatose-1,6-bisphosphate.
- S256 (≠ T259) binding beta-D-fructose 1,6-bisphosphate
- R259 (= R262) binding beta-D-fructose 1,6-bisphosphate; mutation to A: 1.8-fold reduction in substrate affinity and 2.8-fold reduction in catalytic efficiency. 6-fold reduction in substrate affinity and 24-fold reduction in catalytic efficiency; when associated with A-278.
- D278 (= D281) mutation to A: 159-fold reduction in substrate affinity and 2770-fold reduction in catalytic efficiency. 6-fold reduction in substrate affinity and 24-fold reduction in catalytic efficiency; when associated with A-259.
- R280 (= R283) binding beta-D-fructose 1,6-bisphosphate
3gayA Structure of giardia fructose-1,6-biphosphate aldolase in complex with tagatose-1,6-biphosphate (see paper)
54% identity, 96% coverage: 2:311/323 of query aligns to 1:303/319 of 3gayA
- binding 1,6-di-O-phosphono-D-tagatose: N23 (= N24), S49 (= S50), D82 (= D83), H174 (= H181), G175 (= G182), K178 (= K185), H206 (= H213), G207 (= G214), S209 (= S216), N249 (= N256), D251 (= D258), S252 (≠ T259), R255 (= R262)
- binding zinc ion: H83 (= H84), H174 (= H181), H206 (= H213)
3ohiA Structure of giardia fructose-1,6-biphosphate aldolase in complex with 3-hydroxy-2-pyridone (see paper)
54% identity, 96% coverage: 2:311/323 of query aligns to 1:303/319 of 3ohiA
- binding ({3-hydroxy-2-oxo-4-[2-(phosphonooxy)ethyl]pyridin-1(2H)-yl}methyl)phosphonic acid: S49 (= S50), D82 (= D83), H83 (= H84), H174 (= H181), G175 (= G182), K178 (= K185), G207 (= G214), S209 (= S216), N249 (= N256), D251 (= D258), S252 (≠ T259), R255 (= R262)
- binding zinc ion: H83 (= H84), H174 (= H181), H206 (= H213)
3gb6A Structure of giardia fructose-1,6-biphosphate aldolase d83a mutant in complex with fructose-1,6-bisphosphate (see paper)
54% identity, 96% coverage: 2:311/323 of query aligns to 1:302/318 of 3gb6A
- binding 1,6-di-O-phosphono-D-fructose: N23 (= N24), S49 (= S50), H173 (= H181), G174 (= G182), K177 (= K185), H205 (= H213), G206 (= G214), S208 (= S216), N248 (= N256), D250 (= D258), S251 (≠ T259), R254 (= R262)
2isvB Structure of giardia fructose-1,6-biphosphate aldolase in complex with phosphoglycolohydroxamate (see paper)
52% identity, 96% coverage: 2:311/323 of query aligns to 1:292/307 of 2isvB
- binding phosphoglycolohydroxamic acid: D82 (= D83), H168 (= H181), G169 (= G182), K172 (= K185), H195 (= H213), G196 (= G214), S198 (= S216), N238 (= N256), D240 (= D258), S241 (≠ T259)
- binding zinc ion: H83 (= H84), H168 (= H181), H195 (= H213)
1rv8B Class ii fructose-1,6-bisphosphate aldolase from thermus aquaticus in complex with cobalt (see paper)
53% identity, 96% coverage: 3:311/323 of query aligns to 2:305/305 of 1rv8B
- active site: D80 (= D83), H81 (= H84), E140 (= E143), H178 (= H181), H208 (= H213), N251 (= N256)
- binding cobalt (ii) ion: H81 (= H84), E132 (= E135), H178 (= H181), H208 (= H213)
- binding sulfate ion: R116 (≠ K119), H123 (= H126), S211 (= S216), D253 (= D258), T254 (= T259)
2isvA Structure of giardia fructose-1,6-biphosphate aldolase in complex with phosphoglycolohydroxamate (see paper)
51% identity, 96% coverage: 2:311/323 of query aligns to 1:283/298 of 2isvA
3n9sA Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with n-(4-hydroxybutyl)- glycolohydroxamic acid bis- phosphate, a competitive inhibitor (see paper)
46% identity, 95% coverage: 3:310/323 of query aligns to 2:306/307 of 3n9sA
- active site: C69 (≠ L70), E70 (= E71), G136 (= G137), H180 (= H181), A226 (≠ Y229), N253 (= N256)
- binding calcium ion: D104 (= D105), S106 (= S107), E134 (= E135)
- binding 4-{hydroxy[(phosphonooxy)acetyl]amino}butyl dihydrogen phosphate: N23 (= N24), S49 (= S50), D82 (= D83), H83 (= H84), H180 (= H181), G181 (= G182), K184 (= K185), H210 (= H213), G211 (= G214), S213 (= S216), N253 (= N256), D255 (= D258), T256 (= T259)
- binding zinc ion: H83 (= H84), H180 (= H181), H210 (= H213)
3n9rA Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with n-(4-hydroxybutyl)-phosphoglycolohydroxamic acid, a competitive inhibitor (see paper)
45% identity, 95% coverage: 3:310/323 of query aligns to 2:296/297 of 3n9rA
- active site: C69 (≠ L70), E70 (= E71), G136 (= G137), H170 (= H181), A216 (≠ Y229), N243 (= N256)
- binding 2-[hydroxy(4-hydroxybutyl)amino]-2-oxoethyl dihydrogen phosphate: H83 (= H84), H170 (= H181), G171 (= G182), K174 (= K185), H200 (= H213), G201 (= G214), S203 (= S216), N243 (= N256), D245 (= D258), T246 (= T259)
- binding zinc ion: H83 (= H84), H170 (= H181), H200 (= H213)
3c56A Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with n-(3-hydroxypropyl)-glycolohydroxamic acid bisphosphate, a competitive inhibitor (see paper)
45% identity, 95% coverage: 3:310/323 of query aligns to 2:296/297 of 3c56A
- active site: C69 (≠ L70), E70 (= E71), G136 (= G137), H170 (= H181), A216 (≠ Y229), N243 (= N256)
- binding 3-{hydroxy[(phosphonooxy)acetyl]amino}propyl dihydrogen phosphate: N23 (= N24), S49 (= S50), D82 (= D83), H170 (= H181), K174 (= K185), G201 (= G214), S203 (= S216), N243 (= N256), D245 (= D258), T246 (= T259), R249 (= R262)
- binding zinc ion: H83 (= H84), H170 (= H181), H200 (= H213)
3c52A Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with phosphoglycolohydroxamic acid, a competitive inhibitor (see paper)
44% identity, 95% coverage: 3:310/323 of query aligns to 2:295/296 of 3c52A
- active site: C69 (≠ L70), E70 (= E71), G136 (= G137), H169 (= H181), A215 (≠ Y229), N242 (= N256)
- binding calcium ion: D104 (= D105), S106 (= S107), E134 (= E135)
- binding phosphoglycolohydroxamic acid: D82 (= D83), H83 (= H84), H169 (= H181), K173 (= K185), H199 (= H213), G200 (= G214), S202 (= S216), N242 (= N256), D244 (= D258), T245 (= T259)
- binding zinc ion: H83 (= H84), H169 (= H181), H199 (= H213)
3q94A The crystal structure of fructose 1,6-bisphosphate aldolase from bacillus anthracis str. 'Ames ancestor'
45% identity, 96% coverage: 1:311/323 of query aligns to 1:285/285 of 3q94A
- active site: D85 (= D83), H86 (= H84), E145 (= E143), H181 (= H181), H209 (= H213), N231 (= N256)
- binding zinc ion: H86 (= H84), E114 (= E112), H163 (≠ E163), H181 (= H181), H209 (= H213), E235 (≠ D260), E239 (≠ A264)
5uckA Class ii fructose-1,6-bisphosphate aldolase of helicobacter pylori with cleavage products (see paper)
44% identity, 95% coverage: 3:310/323 of query aligns to 2:290/291 of 5uckA
- binding glyceraldehyde-3-phosphate: S49 (= S50), D82 (= D83), H83 (= H84), H164 (= H181), D239 (= D258), R243 (= R262)
- binding zinc ion: H83 (= H84), H83 (= H84), E134 (= E135), H164 (= H181), H194 (= H213), H194 (= H213)
5ucpA Class ii fructose-1,6-bisphosphate aldolase e142a variant of helicobacter pylori with fbp and cleavage products (see paper)
44% identity, 95% coverage: 3:310/323 of query aligns to 2:291/292 of 5ucpA
- binding 1,6-di-O-phosphono-D-fructose: S49 (= S50), D82 (= D83), H83 (= H84), H165 (= H181), K169 (= K185), G196 (= G214), S198 (= S216), N238 (= N256), D240 (= D258), T241 (= T259), R244 (= R262)
- binding zinc ion: H83 (= H84), H83 (= H84), H83 (= H84), E134 (= E135), H165 (= H181), H165 (= H181), H165 (= H181), H195 (= H213),