SitesBLAST
Comparing WP_013402806.1 NCBI__GCF_000166355.1:WP_013402806.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4y2wA Crystal structure of a thermostable alanine racemase from thermoanaerobacter tengcongensis mb4 (see paper)
43% identity, 97% coverage: 13:389/390 of query aligns to 12:387/388 of 4y2wA
- active site: K40 (= K41), R138 (= R139), H168 (= H169), R224 (= R226), Y268 (= Y270), C315 (= C317), D317 (= D319)
- binding alanine: Y268 (= Y270), Y287 (= Y289), C315 (= C317), M316 (= M318)
- binding phosphate ion: N208 (= N210), A209 (≠ S211), I227 (≠ L229)
4lusB Alanine racemase [clostridium difficile 630] (see paper)
43% identity, 98% coverage: 10:390/390 of query aligns to 6:374/374 of 4lusB
- active site: K37 (= K41), H160 (= H169), R213 (= R226), Y258 (= Y270), C304 (= C317), D306 (= D319)
- binding 3,3',3''-phosphanetriyltripropanoic acid: T77 (≠ D81), P79 (= P83), K98 (≠ E102), C124 (≠ K128)
4lutA Alanine racemase [clostridium difficile 630] complex with cycloserine (see paper)
43% identity, 98% coverage: 10:390/390 of query aligns to 6:375/375 of 4lutA
- active site: K37 (= K41), H158 (= H169), R214 (= R226), Y259 (= Y270), C305 (= C317), D307 (= D319)
- binding d-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-n,o-cycloserylamide: K37 (= K41), Y41 (= Y45), L83 (= L87), H158 (= H169), S199 (= S211), R214 (= R226), G216 (= G228), I217 (≠ L229), Y259 (= Y270), Y278 (= Y289), M306 (= M318), Y347 (= Y362)
6q70A Crystal structure of the alanine racemase bsu17640 from bacillus subtilis in the presence of hepes (see paper)
40% identity, 98% coverage: 4:384/390 of query aligns to 2:386/386 of 6q70A
- active site: K39 (= K41), R139 (= R139), H169 (= H169), R225 (= R226), Y272 (= Y270), T319 (≠ C317), D321 (= D319)
- binding pyridoxal-5'-phosphate: V37 (= V39), K39 (= K41), Y43 (= Y45), L85 (= L87), R139 (= R139), H169 (= H169), T210 (≠ S211), R225 (= R226), G227 (= G228), I228 (≠ L229), Y364 (= Y362)
5irpA Crystal structure of the alanine racemase bsu17640 from bacillus subtilis (see paper)
40% identity, 98% coverage: 4:384/390 of query aligns to 2:386/386 of 5irpA
- active site: K39 (= K41), R139 (= R139), H169 (= H169), R225 (= R226), Y272 (= Y270), T319 (≠ C317), D321 (= D319)
- binding magnesium ion: L155 (≠ I155), K156 (≠ A156), S158 (≠ L158), L161 (≠ I161)
- binding 2-amino-2-hydroxymethyl-propane-1,3-diol: D174 (= D174), P235 (= P236), Y364 (= Y362)
- binding (5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate: Y43 (= Y45), L85 (= L87), R139 (= R139), H169 (= H169), N209 (= N210), T210 (≠ S211), R225 (= R226), G227 (= G228), I228 (≠ L229), Y364 (= Y362)
5yycA Crystal structure of alanine racemase from bacillus pseudofirmus (of4) (see paper)
40% identity, 96% coverage: 3:376/390 of query aligns to 2:366/368 of 5yycA
- active site: K41 (= K41), R139 (= R139), H169 (= H169), R225 (= R226), Y269 (= Y270), C316 (= C317), D318 (= D319)
- binding pyridoxal-5'-phosphate: K41 (= K41), Y45 (= Y45), R139 (= R139), H169 (= H169), N209 (= N210), S210 (= S211), R225 (= R226), G227 (= G228), I228 (≠ L229), Y352 (= Y362)
1ftxA Crystal structure of alanine racemase in complex with d-alanine phosphonate
37% identity, 98% coverage: 6:389/390 of query aligns to 3:380/380 of 1ftxA
- active site: K38 (= K41), R135 (= R139), H165 (= H169), R218 (= R226), Y264 (= Y270), C310 (= C317), D312 (= D319)
- binding (1s)-1-[((1e)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylene)amino]ethylphosphonic acid: K38 (= K41), Y42 (= Y45), R135 (= R139), H165 (= H169), N202 (= N210), S203 (= S211), R218 (= R226), G220 (= G228), I221 (≠ L229), Y264 (= Y270), C310 (= C317), M311 (= M318), Y353 (= Y362)
1l6gA Alanine racemase bound with n-(5'-phosphopyridoxyl)-d-alanine (see paper)
37% identity, 98% coverage: 6:389/390 of query aligns to 3:380/382 of 1l6gA
- active site: K38 (= K41), R135 (= R139), H165 (= H169), R218 (= R226), Y264 (= Y270), C310 (= C317), D312 (= D319)
- binding n-(5'-phosphopyridoxyl)-d-alanine: K38 (= K41), Y42 (= Y45), R135 (= R139), H165 (= H169), S203 (= S211), R218 (= R226), G220 (= G228), I221 (≠ L229), Y264 (= Y270), C310 (= C317), M311 (= M318), Y353 (= Y362)
1l6fA Alanine racemase bound with n-(5'-phosphopyridoxyl)-l-alanine (see paper)
37% identity, 98% coverage: 6:389/390 of query aligns to 3:380/382 of 1l6fA
- active site: K38 (= K41), R135 (= R139), H165 (= H169), R218 (= R226), Y264 (= Y270), C310 (= C317), D312 (= D319)
- binding alanyl-pyridoxal-5'-phosphate: K38 (= K41), Y42 (= Y45), R135 (= R139), H165 (= H169), N202 (= N210), S203 (= S211), R218 (= R226), G220 (= G228), I221 (≠ L229), Y264 (= Y270), C310 (= C317), M311 (= M318), Y353 (= Y362)
1epvA Alanine racemase with bound inhibitor derived from d-cycloserine (see paper)
37% identity, 98% coverage: 6:389/390 of query aligns to 3:380/382 of 1epvA
- active site: K38 (= K41), R135 (= R139), H165 (= H169), R218 (= R226), Y264 (= Y270), C310 (= C317), D312 (= D319)
- binding d-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-n,o-cycloserylamide: K38 (= K41), Y42 (= Y45), L84 (= L87), R135 (= R139), H165 (= H169), S203 (= S211), R218 (= R226), G220 (= G228), I221 (≠ L229), Y264 (= Y270), C310 (= C317), M311 (= M318), Y353 (= Y362)
P10724 Alanine racemase; EC 5.1.1.1 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see 8 papers)
37% identity, 98% coverage: 6:389/390 of query aligns to 4:381/388 of P10724
- K39 (= K41) modified: N6-(pyridoxal phosphate)lysine; mutation to A: Loss of activity.
- K129 (= K132) modified: N6-carboxylysine
- R136 (= R139) binding substrate
- H166 (= H169) mutation to A: 6.5-fold decrease in activity.
- R219 (= R226) mutation to A: 100-fold decrease in activity.; mutation to E: 1000-fold decrease in activity.; mutation to K: 4-fold decrease in activity.
- Y265 (= Y270) mutation to A: 5000-fold decrease in activity.; mutation to F: Loss of activity.; mutation to S: 2000-fold decrease in activity.
- M312 (= M318) binding substrate
- Y354 (= Y362) mutation to A: 54-fold increase in serine racemase activity.; mutation to N: 81-fold increase in serine racemase activity.; mutation to Q: 51-fold increase in serine racemase activity.
1bd0A Alanine racemase complexed with alanine phosphonate (see paper)
37% identity, 98% coverage: 6:389/390 of query aligns to 3:380/381 of 1bd0A