SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013403450.1 NCBI__GCF_000166355.1:WP_013403450.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
50% identity, 100% coverage: 1:343/343 of query aligns to 1:343/343 of P30750

query
sites
P30750

M

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x
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40:46 (vs. 40:46, 100% identical) binding ATP
E166 (= E166) mutation to Q: Exhibits little ATPase activity.
VDAPLL 278:283 (≠ TAEPIL 279:284) binding L-methionine
N295 (= N296) mutation to A: Reduces the binding of L-methionine to undetectable levels.
NI 295:296 (≠ NV 296:297) binding L-methionine

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
49% identity, 100% coverage: 1:343/343 of query aligns to 2:344/344 of 3tuzC

query
sites
3tuzC

M

M

I

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L

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N

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K

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x
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x
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N

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L

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D

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F
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x
V

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T

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x
M

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G

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V

binding adenosine-5'-diphosphate: F12 (≠ Y11), Q14 (≠ S13), G42 (= G41), G44 (= G43), K45 (= K44), S46 (= S45), T47 (= T46)
binding selenomethionine: F274 (= F274), V279 (≠ T279), A281 (≠ E281), P282 (= P282), L283 (≠ I283), L284 (= L284), G311 (= G311), M313 (≠ L313)

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
49% identity, 100% coverage: 1:343/343 of query aligns to 2:344/344 of 3tuiC

query
sites
3tuiC

M

M

I

I

R

K

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L

K

S

N

N

L

I

T

T

K

K

I

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Y
x
F

H

H

S

Q

N

G

G

T

I

R

D

T

I

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K

Q

A

A

L

L

D

N

N

N

V

V

N

S

L

L

N

H

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P

K

A

G

G

D

Q

I

I

F

Y

G

G

I

V

I

I

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G

P

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S

S

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G

A
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A

G
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G

K
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K

S
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S

T
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T

L

L

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R

R

C

C

I

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N

N

M

L

L

L

E

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E

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F

F

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Q

H

H

F

F

N

N

L

L

L

L

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T

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K

F

G

G

N

N

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A

A

F

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E

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N

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T

E

E

L

L

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L

L

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G

G

L

L

T

G

N

D

K

K

T

H

D

D

S

S

Y

Y

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P

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S

Q

N

L

L

S

S

G

G

G

G

Q

Q

K

K

Q

Q

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R

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V

G

A

I

I

A

A

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R

A

A

L

L

A

A

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N

N

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P

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K

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A

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K

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H

H

E

E

M

M

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D

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K

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R

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C

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K

C

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A

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G

G

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Q

G

D

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E

E

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F

F

A

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N

H

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A

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N

A

L

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L

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E

F

F

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E

A

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P

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L

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T

S

S

E

V

T

I

A

K

R

K

R

F

F

D

N

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V

D

N

I

N

N

N

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I

L

I

Y

S

G

A

N

Q

I

M

D

D

H

Y

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Q

G

G

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K

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F

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T

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E

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M

R

H

G

G

N

T

N

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K

Q

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D

I

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G

A

V

A

L

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R

A

Y

W

L

L

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Q

E

E

L

H

G

H

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K

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E

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V

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L

D

G

N

Y

V

V

binding adenosine-5'-diphosphate: F12 (≠ Y11), G42 (= G41), A43 (= A42), G44 (= G43), K45 (= K44), S46 (= S45), T47 (= T46)

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
49% identity, 100% coverage: 1:343/343 of query aligns to 2:344/344 of 6cvlD

query
sites
6cvlD

M

M

I

I

R

K

I

L

K

S

N

N

L

I

T

T

K

K

I

V

Y
x
F

H

H

S
x
Q

N

G

G

T

I

R

D

T

I
|
I

K

Q

A

A

L

L

D

N

N

N

V

V

N

S

L

L

N

H

I

V

E

P

K

A

G

G

D

Q

I

I

F

Y

G

G

I

V

I

I

G

G

P

A

S
|
S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

I

R

R

C

C

I

V

N

N

M

L

L

L

E

E

K

R

P

P

T

T

H

E

G

G

S

S

I

V

E

L

I

V

D

D

G

G

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Q

E

E

M

L

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T

K

T

L

L

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S

P

E

T

S

E

E

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L

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T

E

K

M

A

R

R

K

R

K

Q

V

I

G

G

I

M

I

I

F

F

Q

Q

H

H

F

F

N

N

L

L

L

L

S

S

S

S

R

R

T

T

V

V

K

F

G

G

N

N

V

V

A

A

F

L

P

P

L

L

E

E

I

L

A

D

G

N

L

T

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P

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K

K

D

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E

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D

K

N

R

R

R

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V

K

T

E

E

L

L

L

L

E

S

L

L

V

V

G

G

L

L

T

G

N

D

K

K

T

H

D

D

S

S

Y

Y

P

P

S

S

Q
x
N

L

L

S
|
S

G

G

G

G

Q
|
Q

K

K

Q

Q

R

R

V

V

G

A

I

I

A

A

R

R

A

A

L

L

A

A

N

S

N

N

P

P

K

K

V

V

L

L

L

L

C

C

D

D

E

Q

A

A

T

T

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S

A

A

L

L

D

D

P

P

E

A

T

T

T

T

L

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S

S

I

I

L

L

N

E

L

L

L

L

K

K

E

D

I

I

N

N

R

R

E

R

F

L

G

G

I

L

T

T

I

I

V

L

V

L

V

I

T

T

H
|
H

E

E

M

M

N

D

V

V

V

V

K

K

Q

R

I

I

C

C

N

D

K

C

V

V

A

A

V

V

M

I

E

S

K

N

G

G

Q

E

I

L

V

I

E

E

Q

Q

G

D

L

T

L

V

T

S

E

E

I

V

F

F

A

S

N

H

P

P

K

K

T

T

K

P

I

L

A

A

Q

Q

N

K

F

F

L

I

R

Q

S

S

L

T

T

L

F

H

S

L

E

D

L

I

P

P

E

E

E

D

L

Y

K

Q

D

E

K

R

I

L

R

Q

N

A

L

E

H

P

R

F

G

T

H

D

L

C

E

-

W

V

K

P

I

M

V

L

K

R

I

L

N

E

F

F

F

T

G

G

E

Q

V

S

T

V

A

D

E

A

P

P

I

L

L

L

T

S

S

E

V

T

I

A

K

R

K

R

F

F

D

N

V

V

D

N

I

N

N

A

V

I

L

I

Y

S

G

A

N

Q

I

M

D

D

H

Y

I

A

Q

G

G

G

I

V

P

K

Y

F

G

G

T

I

L

M

I

L

V

T

E

E

L

M

R

H

G

G

N

T

N

Q

K

Q

-

D

I

T

E

Q

G

A

V

A

L

I

R

A

Y

W

L

L

Q

Q

E

E

L

H

G

H

L

V

E

K

V

V

E

E

V

V

I

L

D

G

N

Y

V

V

binding phosphothiophosphoric acid-adenylate ester: F12 (≠ Y11), Q14 (≠ S13), I19 (= I18), S41 (= S40), G42 (= G41), A43 (= A42), G44 (= G43), K45 (= K44), S46 (= S45), T47 (= T46), N141 (≠ Q140), S143 (= S142), Q146 (= Q145), H200 (= H199)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
45% identity, 69% coverage: 10:244/343 of query aligns to 7:239/240 of 4ymuJ

query
sites
4ymuJ

I

V

Y

Y

H

K

S

N

N
x
F

G

G

I

-

D

S

I

L

K

E

A
x
V

L

L

D

K

N

G

V

V

N

T

L

L

N

K

I

V

E

N

K

K

G

G

D

E

I

V

F

V

G

V

I

I

I

I

G

G

P

P

S
|
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

L

R

R

C

C

I

I

N

N

M

L

L

L

E

E

K

E

P

P

T

T

H

K

G

G

S

E

I

V

E

F

I

I

D

D

G

G

V

V

E

K

M

I

T

N

K

N

L

-

S

G

P

K

T

V

E

N

L

I

K

N

E

K

M

V

R

R

K

Q

K

K

V

V

G

G

I

M

I

V

F

F

Q

Q

H

H

F

F

N

N

L

L

L

F

S

P

S

H

R

L

T

T

V

A

K

I

G

E

N

N

V

I

A

T

F

L

-

A

P

P

L

V

E

K

I

V

A

K

G

K

L

M

D

N

K

K

K

K

T

E

I

A

D

E

N

E

R

L

V

A

K

V

E

D

L

L

L

L

E

A

L

K

V

V

G

G

L

L

T

L

N

D

K

K

T

K

D

D

S

Q

Y

Y

P

P

S

I

Q

K

L

L

S

S

G

G

G

G

Q

Q

K

K

Q

Q

R

R

V

L

G

A

I

I

A

A

R

R

A

A

L

L

A

A

N

M

N

Q

P

P

K

E

V

V

L

M

L

L

C

F

D

D

E
|
E

A

P

T

T

S

S

A

A

L

L

D

D

P

P

E

E

T

M

T

V

L

K

S

E

I

V

L

L

N

N

L

V

L

M

K

K

E

Q

I

L

N

A

R

N

E

E

F

-

G

G

I

M

T

T

I

M

V

V

V

V

V

V

T

T

H
|
H

E

E

M

M

N

G

V

F

V

A

K

R

Q

E

I

V

C

G

N

D

K

R

V

V

A

I

V

F

M

M

E

D

K

D

G

G

Q

V

I

I

V

V

E

E

Q

E

G

G

L

T

L

P

T

E

E

E

I

I

F

F

A

Y

N

R

P

A

K

K

T

N

K

E

I

R

A

T

Q

R

N

E

F

F

L

L

R

S

S

K

L

I

binding adenosine-5'-triphosphate: F11 (≠ N14), V16 (≠ A20), S36 (= S40), G37 (= G41), S38 (≠ A42), G39 (= G43), K40 (= K44), S41 (= S45), T42 (= T46), E162 (= E166), H194 (= H199)
binding magnesium ion: S41 (= S45), E162 (= E166)

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
42% identity, 71% coverage: 1:244/343 of query aligns to 2:239/241 of 4u00A

query
sites
4u00A

M

I

I

I

R

R

I

I

K

R

N

N

L

L

T

H

K

K

I

W

Y
x
F

H

G

S

P

N

-

G

-

I

-

D

-

I

L

K

H

A
x
V

L

L

D

K

N

G

V

I

N

H

L

L

N

E

I

V

E

A

K

P

G

G

D

E

I

K

F

L

G

V

I

I

I

I

G

G

P

P

S
|
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

I

R

R

C

T

I

I

N

N

M

R

L

L

E

E

K

D

P

F

T

Q

H

E

G

G

S

E

I

V

E

V

I

V

D

D

G

G

V

L

E

S

M

V

T

K

K

-

L

-

S

D

P

D

T

R

E

A

L

L

K

R

E

E

M

I

R

R

K

R

K

E

V

V

G

G

I

M

I

V

F

F

Q

Q

H

Q

F

F

N

N

L

L

L

F

S

P

S

H

R

M

T

T

V

V

K

L

G

E

N

N

V

V

A

T

F

L

-

A

P

P

L

M

E

R

I

V

A

R

G

R

L

W

D

P

K

R

K

E

T

K

I

A

D

E

N

K

R

K

V

A

K

L

E

E

L

L

L

L

E

E

L

R

V

V

G

G

L

I

T

L

N

D

K

Q

T

A

D

R

S

K

Y

Y

P

P

S

A

Q

Q

L

L

S

S

G

G

G

G

Q

Q

K

Q

Q

Q

R

R

V

V

G

A

I

I

A

A

R

R

A

A

L

L

A

A

N

M

N

E

P

P

K

K

V

I

L

M

L

L

C

F

D

D

E

E

A

P

T

T

S

S

A

A

L

L

D

D

P

P

E

E

T

M

T

V

L

G

S

E

I

V

L

L

N

D

L

V

L

M

K

R

E

D

I

L

N

A

R

-

E

Q

F

G

G

G

I

M

T

T

I

M

V

V

V

V

V

V

T

T

H

H

E

E

M

M

N

G

V

F

V

A

K

R

Q

E

I

V

C

A

N

D

K

R

V

V

A

V

V

F

M

M

E

D

K

G

G

G

Q

Q

I

I

V

V

E

E

Q

E

G

G

L

R

L

P

T

E

E

E

I

I

F

F

A

T

N

R

P

P

K

K

T

E

K

E

I

R

A

T

Q

R

N

S

F

F

L

L

R

Q

S

R

L

V

binding adenosine-5'-diphosphate: F12 (≠ Y11), V17 (≠ A20), S37 (= S40), G38 (= G41), S39 (≠ A42), G40 (= G43), K41 (= K44), S42 (= S45), T43 (= T46)

3c4jA Abc protein artp in complex with atp-gamma-s
41% identity, 70% coverage: 1:241/343 of query aligns to 3:238/242 of 3c4jA

query
sites
3c4jA

M

M

I

I

R

D

I

V

K

H

N

Q

L

L

T

K

K

K

I

S

Y
x
F

H

G

S

S

N

-

G

-

I

-

D

-

I

L

K

E

A
x
V

L

L

D

K

N

G

V

I

N

N

L

V

N

H

I

I

E

R

K

E

G

G

D

E

I

V

F

V

G

V

I

V

I

I

G

G

P

P

S
|
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

I

L

R

R

C

C

I

L

N

N

M

L

L

L

E

E

K

D

P

F

T

D

H

E

G

G

S

E

I

I

E

I

I

I

D

D

G

G

V

I

E

N

M

L

T

-

K

K

L

A

S

K

P

D

T

T

E

N

L

L

K

N

E

K

M

V

R

R

K

E

K

E

V

V

G

G

I

M

I

V

F

F

Q

Q

H

R

F

F

N

N

L

L

L

F

S

P

S

H

R

M

T

T

V

V

K

L

G

N

N

N

V

I

A

T

F

L

-

A

P

P

L

M

E

K

I

V

A

R

G

K

L

W

D

P

K

R

K

E

T

K

I

A

D

E

N

A

R

K

V

A

K

M

E

E

L

L

L

L

E

D

L

K

V

V

G

G

L

L

T

K

N

D

K

K

T

A

D

H

S

A

Y

Y

P

P

S

D

Q

S

L

L

S

S

G

G

G

G

Q

Q

K

A

Q

Q

R

R

V

V

G

A

I

I

A

A

R

R

A

A

L

L

A

A

N

M

N

E

P

P

K

K

V

I

L

M

L

L

C

F

D

D

E

E

A

P

T

T

S

S

A

A

L

L

D

D

P

P

E

E

T

M

T

V

L

G

S

E

I

V

L

L

N

S

L

V

L

M

K

K

E

Q

I

L

N

A

R

N

E

E

F

-

G

G

I

M

T

T

I

M

V

V

V

V

V

V

T

T

H

H

E

E

M

M

N

G

V

F

V

A

K

R

Q

E

I

V

C

G

N

D

K

R

V

V

A

L

V

F

M

M

E

D

K

G

G

G

Q

Y

I

I

V

I

E

E

Q

E

G

G

L

K

L

P

T

E

E

D

I

L

F

F

A

D

N

R

P

P

K

Q

T

H

K

E

I

R

A

T

Q

K

N

A

F

F

L

L

binding phosphothiophosphoric acid-adenylate ester: F13 (≠ Y11), V18 (≠ A20), S38 (= S40), G39 (= G41), S40 (≠ A42), G41 (= G43), K42 (= K44), S43 (= S45), T44 (= T46)

3c41J Abc protein artp in complex with amp-pnp/mg2+
41% identity, 70% coverage: 1:241/343 of query aligns to 3:238/242 of 3c41J

query
sites
3c41J

M

M

I

I

R

D

I

V

K

H

N

Q

L

L

T

K

K

K

I

S

Y
x
F

H

G

S

S

N

-

G

-

I

-

D

-

I

L

K

E

A
x
V

L

L

D

K

N

G

V

I

N

N

L

V

N

H

I

I

E

R

K

E

G

G

D

E

I

V

F

V

G

V

I

V

I

I

G

G

P

P

S
|
S

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

I

L

R

R

C

C

I

L

N

N

M

L

L

L

E

E

K

D

P

F

T

D

H

E

G

G

S

E

I

I

E

I

I

I

D

D

G

G

V

I

E

N

M

L

T

-

K

K

L

A

S

K

P

D

T

T

E

N

L

L

K

N

E

K

M

V

R

R

K

E

K

E

V

V

G

G

I

M

I

V

F

F

Q

Q

H

R

F

F

N

N

L

L

L

F

S

P

S

H

R

M

T

T

V

V

K

L

G

N

N

N

V

I

A

T

F

L

-

A

P

P

L

M

E

K

I

V

A

R

G

K

L

W

D

P

K

R

K

E

T

K

I

A

D

E

N

A

R

K

V

A

K

M

E

E

L

L

L

L

E

D

L

K

V

V

G

G

L

L

T

K

N

D

K

K

T

A

D

H

S

A

Y

Y

P

P

S

D

Q

S

L

L

S

S

G

G

G

G

Q

Q

K

A

Q

Q

R

R

V

V

G

A

I

I

A

A

R

R

A

A

L

L

A

A

N

M

N

E

P

P

K

K

V

I

L

M

L

L

C

F

D
|
D

E

E

A

P

T

T

S

S

A

A

L

L

D

D

P

P

E

E

T

M

T

V

L

G

S

E

I

V

L

L

N

S

L

V

L

M

K

K

E

Q

I

L

N

A

R

N

E

E

F

-

G

G

I

M

T

T

I

M

V

V

V

V

V

V

T

T

H

H

E

E

M

M

N

G

V

F

V

A

K

R

Q

E

I

V

C

G

N

D

K

R

V

V

A

L

V

F

M

M

E

D

K

G

G

G

Q

Y

I

I

V

I

E

E

Q

E

G

G

L

K

L

P

T

E

E

D

I

L

F

F

A

D

N

R

P

P

K

Q

T

H

K

E

I

R

A

T

Q

K

N

A

F

F

L

L

binding phosphoaminophosphonic acid-adenylate ester: F13 (≠ Y11), V18 (≠ A20), S38 (= S40), G39 (= G41), G41 (= G43), K42 (= K44), S43 (= S45), T44 (= T46)
binding magnesium ion: S43 (= S45), D163 (= D165)

2olkA Abc protein artp in complex with adp-beta-s
41% identity, 70% coverage: 1:241/343 of query aligns to 3:238/242 of 2olkA

query
sites
2olkA

M

M

I

I

R

D

I

V

K

H

N

Q

L

L

T

K

K

K

I

S

Y
x
F

H

G

S

S

N

-

G

-

I

-

D

-

I

L

K

E

A
x
V

L

L

D

K

N

G

V

I

N

N

L

V

N

H

I

I

E

R

K

E

G

G

D

E

I

V

F

V

G

V

I

V

I

I

G

G

P

P

S
|
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

I

L

R

R

C

C

I

L

N

N

M

L

L

L

E

E

K

D

P

F

T

D

H

E

G

G

S

E

I

I

E

I

I

I

D

D

G

G

V

I

E

N

M

L

T

-

K

K

L

A

S

K

P

D

T

T

E

N

L

L

K

N

E

K

M

V

R

R

K

E

K

E

V

V

G

G

I

M

I

V

F

F

Q

Q

H

R

F

F

N

N

L

L

L

F

S

P

S

H

R

M

T

T

V

V

K

L

G

N

N

N

V

I

A

T

F

L

-

A

P

P

L

M

E

K

I

V

A

R

G

K

L

W

D

P

K

R

K

E

T

K

I

A

D

E

N

A

R

K

V

A

K

M

E

E

L

L

L

L

E

D

L

K

V

V

G

G

L

L

T

K

N

D

K

K

T

A

D

H

S

A

Y

Y

P

P

S

D

Q

S

L

L

S

S

G

G

G

G

Q

Q

K

A

Q

Q

R

R

V

V

G

A

I

I

A

A

R

R

A

A

L

L

A

A

N

M

N

E

P

P

K

K

V

I

L

M

L

L

C

F

D

D

E

E

A

P

T

T

S

S

A

A

L

L

D

D

P

P

E

E

T

M

T

V

L

G

S

E

I

V

L

L

N

S

L

V

L

M

K

K

E

Q

I

L

N

A

R

N

E

E

F

-

G

G

I

M

T

T

I

M

V

V

V

V

V

V

T

T

H

H

E

E

M

M

N

G

V

F

V

A

K

R

Q

E

I

V

C

G

N

D

K

R

V

V

A

L

V

F

M

M

E

D

K

G

G

G

Q

Y

I

I

V

I

E

E

Q

E

G

G

L

K

L

P

T

E

E

D

I

L

F

F

A

D

N

R

P

P

K

Q

T

H

K

E

I

R

A

T

Q

K

N

A

F

F

L

L

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (≠ Y11), V18 (≠ A20), S38 (= S40), G39 (= G41), S40 (≠ A42), G41 (= G43), K42 (= K44), S43 (= S45), T44 (= T46)

2oljA Abc protein artp in complex with adp/mg2+
41% identity, 70% coverage: 1:241/343 of query aligns to 3:238/242 of 2oljA

query
sites
2oljA

M

M

I

I

R

D

I

V

K

H

N

Q

L

L

T

K

K

K

I

S

Y
x
F

H

G

S

S

N

-

G

-

I

-

D

-

I

L

K

E

A
x
V

L

L

D

K

N

G

V

I

N

N

L

V

N

H

I

I

E

R

K

E

G

G

D

E

I

V

F

V

G

V

I

V

I

I

G

G

P

P

S
|
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

I

L

R

R

C

C

I

L

N

N

M

L

L

L

E

E

K

D

P

F

T

D

H

E

G

G

S

E

I

I

E

I

I

I

D

D

G

G

V

I

E

N

M

L

T

-

K

K

L

A

S

K

P

D

T

T

E

N

L

L

K

N

E

K

M

V

R

R

K

E

K

E

V

V

G

G

I

M

I

V

F

F

Q

Q

H

R

F

F

N

N

L

L

L

F

S

P

S

H

R

M

T

T

V

V

K

L

G

N

N

N

V

I

A

T

F

L

-

A

P

P

L

M

E

K

I

V

A

R

G

K

L

W

D

P

K

R

K

E

T

K

I

A

D

E

N

A

R

K

V

A

K

M

E

E

L

L

L

L

E

D

L

K

V

V

G

G

L

L

T

K

N

D

K

K

T

A

D

H

S

A

Y

Y

P

P

S

D

Q

S

L

L

S

S

G

G

G

G

Q

Q

K

A

Q

Q

R

R

V

V

G

A

I

I

A

A

R

R

A

A

L

L

A

A

N

M

N

E

P

P

K

K

V

I

L

M

L

L

C

F

D

D

E

E

A

P

T

T

S

S

A

A

L

L

D

D

P

P

E

E

T

M

T

V

L

G

S

E

I

V

L

L

N

S

L

V

L

M

K

K

E

Q

I

L

N

A

R

N

E

E

F

-

G

G

I

M

T

T

I

M

V

V

V

V

V

V

T

T

H

H

E

E

M

M

N

G

V

F

V

A

K

R

Q

E

I

V

C

G

N

D

K

R

V

V

A

L

V

F

M

M

E

D

K

G

G

G

Q

Y

I

I

V

I

E

E

Q

E

G

G

L

K

L

P

T

E

E

D

I

L

F

F

A

D

N

R

P

P

K

Q

T

H

K

E

I

R

A

T

Q

K

N

A

F

F

L

L

binding adenosine-5'-diphosphate: F13 (≠ Y11), V18 (≠ A20), S38 (= S40), G39 (= G41), S40 (≠ A42), G41 (= G43), K42 (= K44), S43 (= S45), T44 (= T46)

8j5qD Cryo-em structure of mycobacterium tuberculosis oppabcd in the pre- translocation state (see paper)
39% identity, 70% coverage: 1:241/343 of query aligns to 348:599/611 of 8j5qD

query
sites
8j5qD

M

V

I

V

R

R

I

V

K

R

N

H

L

L

T

V

K

K

I

T

Y

Y

H

R

-

L

S

A

N

K

G

G

I

V

-

V

-

L

-

R

-

R

-

A

-

I

-

G

D

E

I

V

K

R

A

A

L

V

D

D

N

G

V

I

N

S

L

L

N

E

I

L

E

R

K

Q

G

G

D

R

I

T

F

L

G

G

I

I

I

V

G

G

P

E

S

S

G

G

A

S

G

G

K

K

S

S

T

T

L

T

I

L

R

H

C

E

I

I

N

L

M

E

L

L

E

A

K

A

P

P

T

Q

H

S

G

G

S

S

I

I

E

E

I

V

D

L

G

G

V

T

E

D

M

V

T

A

K

T

L

L

S

G

P

T

T

A

E

E

L

R

K

R

E

S

M

L

R

R

K

R

K

D

V

I

G

Q

I

V

I

V

F

F

Q

Q

H

D

-

P

-

V

F

A

N

S

L

L

L

D

S

P

S

R

R

L

T

P

V

V

K

F

G

D

N

L

V

I

A

A

F

E

P

P

L

L

E

Q

I

A

A

N

G

G

L

F

D

G

K

K

K

N

T

E

I

T

D

H

N

A

R

R

V

V

K

A

E

E

L

L

L

L

E

D

L

I

V

V

G

G

L

L

T

R

N

H

K

G

T

D

D

A

S

S

-

R

Y

Y

P

P

S

A

Q

E

L

F

S

S

G

G

G

G

Q

Q

K

K

Q

Q

R

R

V

I

G

G

I

I

A

A

R

R

A

A

L

L

A

A

N

L

N

Q

P

P

K

K

V

I

L

L

L

A

C

L

D

D

E

E

A

P

T

V

S

S

A

A

L

L

D

D

P

V

E

S

T

I

T

Q

L

A

S

G

I

I

L

I

N

N

L

L

L

L

K

L

E

D

I

L

N

Q

R

E

E

Q

F

F

G

G

I

L

T

S

I

Y

V

L

V

F

V

V

T

S

H

H

E

D

M

L

N

S

V

V

V

V

K

K

Q

H

I

L

C

A

N

H

K

Q

V

V

A

A

V

V

M

M

E

L

K

A

G

G

Q

T

I

V

V

V

E

E

Q

Q

G

G

L

D

L

S

T

E

E

E

I

V

F

F

A

G

N

N

P

P

K

K

T

H

K

E

I

Y

A

T

Q

R

N

R

F

L

L

L

Sites not aligning to the query:

binding iron/sulfur cluster: 285, 291, 298, 316, 318

A5U7B7 Cell division ATP-binding protein FtsE from Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra) (see 2 papers)
42% identity, 64% coverage: 1:221/343 of query aligns to 1:218/229 of A5U7B7

query
sites
A5U7B7

M

M

I

I

R

T

I

L

K

D

N

H

L

V

T

T

K

K

I

Q

Y

Y

H

K

S

S

N

S

G

A

I

R

D

-

I

-

K

P

A

A

L

L

D

D

N

D

V

I

N

N

L

V

N

K

I

I

E

D

K

K

G

G

D

E

I

F

F

V

G

F

I

L

I

I

G

G

P

P

S

S

G

G

A

S

G

G

K
|
K

S

S

T

T

L

F

I

M

R

R

C

L

I

L

N

L

M

A

L

A

E

E

K

T

P

P

T

T

H

S

G

G

S

D

I

V

E

R

I

V

D

S

G

K

V

F

E

H

M

V

T

N

K

K

L

L

S

R

P

G

T

R

E

H

L

V

K

P

E

K

M

L

R

R

K

Q

K

V

V

I

G

G

I
x
C

I

V

F

F

Q

Q

H

D

F

F

N

R

L

L

L

L

S

Q

S

Q

R

K

T

T

V

V

K

Y

G

D

N

N

V

V

A

A

F

F

P

A

L

L

E

E

I

V

A

I

G

G

L

K

D

R

K

T

K

D

T

A

I

I

D

N

N

R

R

V

V

V

K

P

E

E

L

V

L

L

E

E

L

T

V

V

G

G

L

L

T

S

N

G

K

K

T

A

D

N

S

R

Y

L

P

P

S

D

Q

E

L

L

S

S

G

G

G

G

Q

E

K

Q

Q

Q

R

R

V

V

G

A

I

I

A

A

R

R

A

A

L

F

A

V

N

N

N

R

P

P

K

L

V

V

L

L

L

L

C

A

D

D

E

E

A

P

T

T

S

G

A

N

L

L

D

D

P

P

E

E

T

T

T

S

L

R

S

D

I

I

L

M

N

D

L

L

L

L

K

E

E

R

I

I

N

N

R

R

E

T

F

-

G

G

I

T

T

T

I

V

V

L

V

M

V

A

T

T

H

H

E

D

M

H

N

H

V

I

V

V

K

D

Q

S

I

M

C

R

N

Q

K

R

V

V

A

V

V

E

M

L

E

S

K

L

G

G

Q

R

I

L

V

V

E

R

K42 (= K44) mutation to R: Does not bind ATP. Does not affect dimerization.
C84 (≠ I86) mutation to A: Does not affect ATPase activity.

8igqA Cryo-em structure of mycobacterium tuberculosis adp bound ftsex/ripc complex in peptidisc (see paper)
42% identity, 64% coverage: 1:221/343 of query aligns to 2:219/227 of 8igqA

query
sites
8igqA

M

M

I

I

R

T

I

L

K

D

N

H

L

V

T

T

K

K

I

Q

Y
|
Y

H

K

S

S

N

S

G

A

I

R

D

-

I

-

K

P

A

A

L

L

D

D

N

D

V

I

N

N

L

V

N

K

I

I

E

D

K

K

G

G

D

E

I

F

F

V

G

F

I

L

I

I

G

G

P

P

S

S

G
|
G

A
x
S

G

G

K
|
K

S
|
S

T

T

L

F

I

M

R

R

C

L

I

L

N

L

M

A

L

A

E

E

K

T

P

P

T

T

H

S

G

G

S

D

I

V

E

R

I

V

D

S

G

K

V

F

E

H

M

V

T

N

K

K

L

L

S

R

P

G

T

R

E

H

L

V

K

P

E

K

M

L

R

R

K

Q

K

V

V

I

G

G

I

C

I

V

F

F

Q

Q

H

D

F

F

N

R

L

L

L

L

S

Q

S

Q

R

K

T

T

V

V

K

Y

G

D

N

N

V

V

A

A

F

F

P

A

L

L

E

E

I

V

A

I

G

G

L

K

D

R

K

T

K

D

T

A

I

I

D

N

N

R

R

V

V

V

K

P

E

E

L

V

L

L

E

E

L

T

V

V

G

G

L

L

T

S

N

G

K

K

T

A

D

N

S

R

Y

L

P

P

S

D

Q

E

L

L

S

S

G

G

G

G

Q

E

K

Q

Q

Q

R

R

V

V

G

A

I

I

A

A

R

R

A

A

L

F

A

V

N

N

N

R

P

P

K

L

V

V

L

L

L

L

C

A

D

D

E

E

A

P

T

T

S

G

A

N

L

L

D

D

P

P

E

E

T

T

T

S

L

R

S

D

I

I

L

M

N

D

L

L

L

L

K

E

E

R

I

I

N

N

R

R

E

T

F

-

G

G

I

T

T

T

I

V

V

L

V

M

V

A

T

T

H

H

E

D

M

H

N

H

V

I

V

V

K

D

Q

S

I

M

C

R

N

Q

K

R

V

V

A

V

V

E

M

L

E

S

K

L

G

G

Q

R

I

L

V

V

E

R

binding adenosine-5'-diphosphate: Y12 (= Y11), G40 (= G41), S41 (≠ A42), K43 (= K44), S44 (= S45)

8iddA Cryo-em structure of mycobacterium tuberculosis atp bound ftsex/ripc complex in peptidisc (see paper)
42% identity, 64% coverage: 1:221/343 of query aligns to 2:219/225 of 8iddA

query
sites
8iddA

M

M

I

I

R

T

I

L

K

D

N

H

L

V

T

T

K

K

I

Q

Y
|
Y

H

K

S

S

N

S

G

A

I

R

D

-

I

-

K

P

A

A

L

L

D

D

N

D

V

I

N

N

L

V

N

K

I

I

E

D

K

K

G

G

D

E

I

F

F

V

G

F

I

L

I

I

G

G

P

P

S
|
S

G

G

A

S

G
|
G

K
|
K

S
|
S

T

T

L

F

I

M

R

R

C

L

I

L

N

L

M

A

L

A

E

E

K

T

P

P

T

T

H

S

G

G

S

D

I

V

E

R

I

V

D

S

G

K

V

F

E

H

M

V

T

N

K

K

L

L

S

R

P

G

T

R

E

H

L

V

K

P

E

K

M

L

R

R

K

Q

K

V

V

I

G

G

I

C

I

V

F

F

Q

Q

H

D

F

F

N

R

L

L

L

L

S

Q

S

Q

R

K

T

T

V

V

K

Y

G

D

N

N

V

V

A

A

F

F

P

A

L

L

E

E

I

V

A

I

G

G

L

K

D

R

K

T

K

D

T

A

I

I

D

N

N

R

R

V

V

V

K

P

E

E

L

V

L

L

E

E

L

T

V

V

G

G

L

L

T

S

N

G

K

K

T

A

D

N

S

R

Y

L

P

P

S

D

Q

E

L

L

S

S

G

G

G

G

Q

E

K

Q

Q

Q

R

R

V

V

G

A

I

I

A

A

R

R

A

A

L

F

A

V

N

N

N

R

P

P

K

L

V

V

L

L

L

L

C

A

D

D

E

E

A

P

T

T

S

G

A

N

L

L

D

D

P

P

E

E

T

T

T

S

L

R

S

D

I

I

L

M

N

D

L

L

L

L

K

E

E

R

I

I

N

N

R

R

E

T

F

-

G

G

I

T

T

T

I

V

V

L

V

M

V

A

T

T

H

H

E

D

M

H

N

H

V

I

V

V

K

D

Q

S

I

M

C

R

N

Q

K

R

V

V

A

V

V

E

M

L

E

S

K

L

G

G

Q

R

I

L

V

V

E

R

binding adenosine-5'-triphosphate: Y12 (= Y11), S39 (= S40), G42 (= G43), K43 (= K44), S44 (= S45)

8j5tD Cryo-em structure of mycobacterium tuberculosis oppabcd in the catalytic intermediate state (see paper)
38% identity, 70% coverage: 1:241/343 of query aligns to 348:599/608 of 8j5tD

query
sites
8j5tD

M

V

I

V

R

R

I

V

K

R

N

H

L

L

T

V

K

K

I

T

Y
|
Y

H

R

-

L

S

A

N

K

G

G

I

V

-

V

-

L

-

R

-

R

-

A

-

I

-

G

D

E

I

V

K

R

A

A

L

V

D

D

N

G

V

I

N

S

L

L

N

E

I

L

E

R

K

Q

G

G

D

R

I

T

F

L

G

G

I

I

I

V

G

G

P

E

S
|
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

T

I

L

R

H

C

E

I

I

N

L

M

E

L

L

E

A

K

A

P

P

T

Q

H

S

G

G

S

S

I

I

E

E

I

V

D

L

G

G

V

T

E

D

M

V

T

A

K

T

L

L

S

G

P

T

T

A

E

E

L

R

K

R

E

S

M

L

R

R

K

R

K

D

V

I

G

Q

I

V

I

V

F

F

Q
|
Q

H

D

-

P

-

V

F

A

N

S

L

L

L

D

S

P

S

R

R

L

T

P

V

V

K

F

G

D

N

L

V

I

A

A

F

E

P

P

L

L

E

Q

I

A

A

N

G

G

L

F

D

G

K

K

K

N

T

E

I

T

D

H

N

A

R

R

V

V

K

A

E

E

L

L

L

L

E

D

L

I

V

V

G

G

L

L

T

R

N

H

K

G

T

D

D

A

S

S

-
x
R

Y

Y

P

P

S

A

Q
x
E

L

F

S
|
S

G

G

G
|
G

Q

Q

K

K

Q

Q

R

R

V

I

G

G

I

I

A

A

R

R

A

A

L

L

A

A

N

L

N

Q

P

P

K

K

V

I

L

L

L

A

C

L

D

D

E

D

A

P

T

V

S

S

A

A

L

L

D

D

P

V

E

S

T

I

T

Q

L

A

S

G

I

I

L

I

N

N

L

L

L

L

K

L

E

D

I

L

N

Q

R

E

E

Q

F

F

G

G

I

L

T

S

I

Y

V

L

V

F

V

V

T

S

H
|
H

E

D

M

L

N

S

V

V

V

V

K

K

Q

H

I

L

C

A

N

H

K

Q

V

V

A

A

V

V

M

M

E

L

K

A

G

G

Q

T

I

V

V

V

E

E

Q

Q

G

G

L

D

L

S

T

E

E

E

I

V

F

F

A

G

N

N

P

P

K

K

T

H

K

E

I

Y

A

T

Q

R

N

R

F

L

L

L

binding adenosine-5'-triphosphate: Y358 (= Y11), S395 (= S40), G396 (= G41), S397 (≠ A42), G398 (= G43), K399 (= K44), S400 (= S45), T401 (= T46), Q444 (= Q89), R494 (vs. gap), E498 (≠ Q140), S500 (= S142), G502 (= G144), H557 (= H199)
binding magnesium ion: S400 (= S45), Q444 (= Q89)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 13, 42, 43, 45, 46, 47, 48, 95, 146, 153, 155, 157, 158, 212
binding magnesium ion: 47, 95
binding iron/sulfur cluster: 248, 285, 288, 291, 298, 316, 318

8j5sD Cryo-em structure of mycobacterium tuberculosis oppabcd in the pre- catalytic intermediate state (see paper)
38% identity, 70% coverage: 1:241/343 of query aligns to 348:599/608 of 8j5sD

query
sites
8j5sD

M

V

I

V

R

R

I

V

K

R

N

H

L

L

T

V

K

K

I

T

Y
|
Y

H

R

-

L

S

A

N

K

G

G

I

V

-

V

-

L

-

R

-

R

-

A

-

I

-

G

D

E

I

V

K

R

A
|
A

L

V

D

D

N

G

V

I

N

S

L

L

N

E

I

L

E

R

K

Q

G

G

D

R

I

T

F

L

G

G

I

I

I

V

G

G

P

E

S

S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

T

I

L

R

H

C

E

I

I

N

L

M

E

L

L

E

A

K

A

P

P

T

Q

H

S

G

G

S

S

I

I

E

E

I

V

D

L

G

G

V

T

E

D

M

V

T

A

K

T

L

L

S

G

P

T

T

A

E

E

L

R

K

R

E

S

M

L

R

R

K

R

K

D

V

I

G

Q

I

V

I

V

F

F

Q
|
Q

H

D

-

P

-

V

F

A

N

S

L

L

L

D

S

P

S

R

R

L

T

P

V

V

K

F

G

D

N

L

V

I

A

A

F

E

P

P

L

L

E

Q

I

A

A

N

G

G

L

F

D

G

K

K

K

N

T

E

I

T

D

H

N

A

R

R

V

V

K

A

E

E

L

L

L

L

E

D

L

I

V

V

G

G

L

L

T

R

N

H

K

G

T

D

D

A

S

S

-

R

Y

Y

P

P

S

A

Q

E

L

F

S

S

G

G

G

G

Q

Q

K

K

Q

Q

R

R

V

I

G

G

I

I

A

A

R

R

A

A

L

L

A

A

N

L

N

Q

P

P

K

K

V

I

L

L

L

A

C

L

D

D

E

D

A

P

T

V

S

S

A

A

L

L

D

D

P

V

E

S

T

I

T

Q

L

A

S

G

I

I

L

I

N

N

L

L

L

L

K

L

E

D

I

L

N

Q

R

E

E

Q

F

F

G

G

I

L

T

S

I

Y

V

L

V

F

V

V

T

S

H

H

E

D

M

L

N

S

V

V

V

V

K

K

Q

H

I

L

C

A

N

H

K

Q

V

V

A

A

V

V

M

M

E

L

K

A

G

G

Q

T

I

V

V

V

E

E

Q

Q

G

G

L

D

L

S

T

E

E

E

I

V

F

F

A

G

N

N

P

P

K

K

T

H

K

E

I

Y

A

T

Q

R

N

R

F

L

L

L

binding phosphoaminophosphonic acid-adenylate ester: Y358 (= Y11), A375 (= A20), G396 (= G41), S397 (≠ A42), G398 (= G43), K399 (= K44), S400 (= S45), T401 (= T46), Q444 (= Q89)
binding magnesium ion: S400 (= S45), Q444 (= Q89)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 13, 20, 22, 41, 42, 43, 44, 45, 46, 47, 48, 60, 95, 278
binding magnesium ion: 47, 95
binding iron/sulfur cluster: 285, 291, 298, 316, 318

6z4wA Ftse structure from streptococcus pneumoniae in complex with adp (space group p 1) (see paper)
40% identity, 64% coverage: 1:221/343 of query aligns to 3:219/230 of 6z4wA

query
sites
6z4wA

M

I

I

I

R

E

I

M

K

R

N

D

L

V

T

V

K

K

I

K

Y
|
Y

H

-

S

D

N

N

G

G

I
x
T

D

-

I

-

K

T

A
|
A

L

L

D

R

N

G

V

V

N

S

L

V

N

S

I

V

E

Q

K

P

G

G

D

E

I

F

F

A

G

Y

I

I

I

V

G

G

P

P

S

S

G
|
G

A

A

G
|
G

K
|
K

S
|
S

T
|
T

L

F

I

I

R

R

C

S

I

L

N

Y

M

R

L

E

E

V

K

K

P

I

T

D

H

K

G

G

S

S

I

L

E

S

I

V

D

A

G

G

V

F

E

N

M

L

T

V

K

K

L

I

S

K

P

K

T

K

E

D

L

V

K

P

E

L

M

L

R

R

K

R

K

S

V

V

G

G

I

V

I

V

F

F

Q

Q

H

D

F

Y

N

K

L

L

L

L

S

P

S

K

R

K

T

T

V

V

K

Y

G

E

N

N

V

I

A

A

F

Y

P

A

L

M

E

E

I

V

A

I

G

G

L

E

D

N

K

R

K

R

T

N

I

I

D

K

N

R

R

R

V

V

K

M

E

E

L

V

L

L

E

D

L

L

V

V

G

G

L

L

T

K

N

H

K

K

T

V

D

R

S

S

Y

F

P

P

S

N

Q

E

L

L

S

S

G

G

G

G

Q

E

K

Q

Q

Q

R

R

V

I

G

A

I

I

A

A

R

R

A

A

L

I

A

V

N

N

N

N

P

P

K

K

V

V

L

L

L

I

C

A

D

D

E

E

A

P

T

T

S

G

A

N

L

L

D

D

P

P

E

D

T

N

T

S

L

W

S

E

I

I

L

M

N

N

L

L

L

L

K

E

E

R

I

I

N

N

R

L

E

Q

F

-

G

G

I

T

T

T

I

I

V

L

V

M

V

A

T

T

H

H

E

N

M

S

N

Q

V

I

V

V

K

N

Q

T

I

L

C

R

N

H

K

R

V

V

A

I

V

A

M

I

E

E

K

N

G

G

Q

R

I

V