SitesBLAST
Comparing WP_013403985.1 NCBI__GCF_000166355.1:WP_013403985.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P19414 Aconitate hydratase, mitochondrial; Aconitase; Citrate hydro-lyase; EC 4.2.1.3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 2 papers)
36% identity, 99% coverage: 3:639/643 of query aligns to 59:774/778 of P19414
- R604 (= R493) mutation to K: Strongly diminishes the catalytic activity towards both known substrates, aconitate and homoaconitate.
Sites not aligning to the query:
- 1:16 modified: transit peptide, Mitochondrion
P39533 Homocitrate dehydratase, mitochondrial; Aconitase 2; EC 4.2.1.- from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
36% identity, 93% coverage: 3:599/643 of query aligns to 54:737/789 of P39533
- K610 (≠ R493) mutation to R: Reduces catalytic activity towards homoaconitate by 45% and increases the activity towards aconitate by a factor 116.
5acnA Structure of activated aconitase. Formation of the (4fe-4s) cluster in the crystal (see paper)
35% identity, 100% coverage: 3:643/643 of query aligns to 36:754/754 of 5acnA
- active site: D100 (= D64), H101 (= H65), D165 (= D119), R447 (= R379), S642 (= S533), R644 (= R535)
- binding fe3-s4 cluster: I145 (= I99), H147 (= H101), H167 (= H121), C358 (= C297), C421 (= C357), C424 (= C360), N446 (= N378)
- binding tricarballylic acid: K198 (≠ I152), G235 (= G189), R666 (= R557)
P16276 Aconitate hydratase, mitochondrial; Aconitase; Citrate hydro-lyase; EC 4.2.1.3 from Sus scrofa (Pig) (see 3 papers)
35% identity, 100% coverage: 3:643/643 of query aligns to 63:781/781 of P16276
- Q99 (= Q35) binding substrate
- DSH 192:194 (= DSH 119:121) binding substrate
- C385 (= C297) binding [4Fe-4S] cluster
- C448 (= C357) binding [4Fe-4S] cluster
- C451 (= C360) binding [4Fe-4S] cluster
- R474 (= R379) binding substrate
- R479 (= R384) binding substrate
- R607 (= R493) binding substrate
- SR 670:671 (= SR 534:535) binding substrate
Sites not aligning to the query:
- 28 modified: Pyrrolidone carboxylic acid
1b0kA S642a:fluorocitrate complex of aconitase (see paper)
35% identity, 100% coverage: 3:643/643 of query aligns to 35:753/753 of 1b0kA
- active site: D99 (= D64), H100 (= H65), D164 (= D119), R446 (= R379), A641 (≠ S533), R643 (= R535)
- binding citrate anion: Q71 (= Q35), H100 (= H65), D164 (= D119), S165 (= S120), R446 (= R379), R451 (= R384), R579 (= R493), A641 (≠ S533), S642 (= S534), R643 (= R535)
- binding oxygen atom: D164 (= D119), H166 (= H121)
- binding iron/sulfur cluster: H100 (= H65), D164 (= D119), H166 (= H121), S356 (= S296), C357 (= C297), C420 (= C357), C423 (= C360)
8acnA Crystal structures of aconitase with isocitrate and nitroisocitrate bound (see paper)
35% identity, 100% coverage: 3:643/643 of query aligns to 35:753/753 of 8acnA
- active site: D99 (= D64), H100 (= H65), D164 (= D119), R446 (= R379), S641 (= S533), R643 (= R535)
- binding nitroisocitric acid: Q71 (= Q35), T74 (= T38), H100 (= H65), D164 (= D119), S165 (= S120), R446 (= R379), R451 (= R384), R579 (= R493), S641 (= S533), S642 (= S534), R643 (= R535)
- binding iron/sulfur cluster: H100 (= H65), D164 (= D119), H166 (= H121), S356 (= S296), C357 (= C297), C420 (= C357), C423 (= C360), I424 (= I361)
1fghA Complex with 4-hydroxy-trans-aconitate (see paper)
35% identity, 100% coverage: 3:643/643 of query aligns to 35:753/753 of 1fghA