SitesBLAST
Comparing WP_013417865.1 NCBI__GCF_000166055.1:WP_013417865.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6zzpA Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from psychrobacter arcticus complexed with NAD+ and 3-oxovalerate (see paper)
64% identity, 100% coverage: 2:258/258 of query aligns to 7:265/265 of 6zzpA
- binding nicotinamide-adenine-dinucleotide: G17 (= G12), S20 (≠ G15), G21 (= G16), I22 (= I17), D41 (= D36), I42 (≠ V37), M66 (= M60), D67 (= D61), V68 (= V62), N94 (= N88), A95 (= A89), G96 (= G90), M145 (= M138), S147 (= S140), Y160 (= Y153), K164 (= K157), P190 (= P183), F192 (= F185), V193 (= V186), T195 (= T188), L197 (= L190), V198 (= V191)
- binding 3-oxidanylidenepentanoic acid: Q98 (= Q92), S147 (= S140), H149 (= H142), K157 (= K150), Y160 (= Y153), F192 (= F185), Q201 (= Q194)
6zzoC Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from psychrobacter arcticus complexed with NAD+ and acetoacetate (see paper)
64% identity, 100% coverage: 2:258/258 of query aligns to 7:265/265 of 6zzoC
- binding acetoacetic acid: Q98 (= Q92), H149 (= H142), K157 (= K150), F192 (= F185), Q201 (= Q194)
- binding nicotinamide-adenine-dinucleotide: G17 (= G12), S20 (≠ G15), G21 (= G16), I22 (= I17), D41 (= D36), I42 (≠ V37), M66 (= M60), D67 (= D61), V68 (= V62), N94 (= N88), A95 (= A89), G96 (= G90), M145 (= M138), Y160 (= Y153), K164 (= K157), P190 (= P183), F192 (= F185), V193 (= V186), T195 (= T188), L197 (= L190), V198 (= V191)
8dt1C Crystal structure of a putative d-beta-hydroxybutyrate dehydrogenase from burkholderia cenocepacia j2315 in complex with NAD
63% identity, 99% coverage: 2:257/258 of query aligns to 1:258/259 of 8dt1C
- binding nicotinamide-adenine-dinucleotide: G11 (= G12), S14 (≠ G15), G15 (= G16), I16 (= I17), D35 (= D36), L36 (≠ V37), M60 (= M60), V62 (= V62), N88 (= N88), A89 (= A89), M139 (= M138), Y154 (= Y153), K158 (= K157), P184 (= P183), G185 (= G184), F186 (= F185), V187 (= V186), T189 (= T188)
6zzqA Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from acinetobacter baumannii complexed with NAD+ and acetoacetate (see paper)
42% identity, 98% coverage: 3:255/258 of query aligns to 4:257/260 of 6zzqA
- active site: G17 (= G16), S142 (= S140), Y155 (= Y153)
- binding acetoacetic acid: Q94 (= Q92), S142 (= S140), K152 (= K150), Y155 (= Y153), Q196 (= Q194)
- binding nicotinamide-adenine-dinucleotide: G13 (= G12), S16 (≠ G15), G17 (= G16), I18 (= I17), D37 (= D36), M38 (≠ V37), D63 (= D61), V64 (= V62), N90 (= N88), A91 (= A89), G92 (= G90), M140 (= M138), A141 (≠ G139), S142 (= S140), Y155 (= Y153), K159 (= K157), Y187 (≠ F185), V188 (= V186), T190 (= T188)
6zzsD Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from acinetobacter baumannii complexed with NAD+ and 3-oxovalerate (see paper)
42% identity, 98% coverage: 3:255/258 of query aligns to 5:258/261 of 6zzsD
- active site: G18 (= G16), S143 (= S140), Y156 (= Y153)
- binding nicotinamide-adenine-dinucleotide: G14 (= G12), S17 (≠ G15), I19 (= I17), D38 (= D36), M39 (≠ V37), D64 (= D61), V65 (= V62), N91 (= N88), A92 (= A89), G93 (= G90), M141 (= M138), A142 (≠ G139), S143 (= S140), Y156 (= Y153), K160 (= K157), P186 (= P183), G187 (= G184), V189 (= V186), T191 (= T188), L193 (= L190)
- binding 3-oxidanylidenepentanoic acid: Q95 (= Q92), S143 (= S140), N145 (≠ H142), K153 (= K150), Y156 (= Y153), Q197 (= Q194)
5b4tA Crystal structure of d-3-hydroxybutyrate dehydrogenase from alcaligenes faecalis complexed with NAD+ and a substrate d-3- hydroxybutyrate (see paper)
39% identity, 98% coverage: 3:255/258 of query aligns to 2:257/260 of 5b4tA
- active site: G15 (= G16), N114 (≠ H112), S142 (= S140), Y155 (= Y153), K159 (= K157), I200 (≠ Q198)
- binding (3R)-3-hydroxybutanoic acid: Q94 (= Q92), S142 (= S140), H144 (= H142), K152 (= K150), Y155 (= Y153), W187 (≠ F185), Q196 (= Q194)
- binding nicotinamide-adenine-dinucleotide: G11 (= G12), T13 (≠ A14), G15 (= G16), I16 (= I17), F36 (≠ V37), D63 (= D61), L64 (≠ V62), N90 (= N88), G92 (= G90), L113 (≠ I111), I140 (≠ M138), Y155 (= Y153), K159 (= K157), P185 (= P183), G186 (= G184), W187 (≠ F185), V188 (= V186), T190 (= T188), L192 (= L190), V193 (= V191)
3w8dA Crystal structure of d-3-hydroxybutyrate dehydrogenase from alcaligenes faecalis complexed with NAD+ and an inhibitor methylmalonate
39% identity, 98% coverage: 3:255/258 of query aligns to 2:257/260 of 3w8dA
- active site: G15 (= G16), N114 (≠ H112), S142 (= S140), Y155 (= Y153), K159 (= K157), I200 (≠ Q198)
- binding methylmalonic acid: Q94 (= Q92), S142 (= S140), H144 (= H142), K152 (= K150), Y155 (= Y153), W187 (≠ F185), Q196 (= Q194), W257 (= W255)
- binding nicotinamide-adenine-dinucleotide: G11 (= G12), T13 (≠ A14), S14 (≠ G15), G15 (= G16), I16 (= I17), F36 (≠ V37), A62 (≠ M60), D63 (= D61), L64 (≠ V62), N90 (= N88), A91 (= A89), G92 (= G90), L113 (≠ I111), S142 (= S140), Y155 (= Y153), K159 (= K157), P185 (= P183), G186 (= G184), W187 (≠ F185), V188 (= V186), T190 (= T188), L192 (= L190), V193 (= V191)
3vdrA Crystal structure of d-3-hydroxybutyrate dehydrogenase, prepared in the presence of the substrate d-3-hydroxybutyrate and NAD(+) (see paper)
39% identity, 98% coverage: 3:255/258 of query aligns to 2:257/260 of 3vdrA
- active site: G15 (= G16), N114 (≠ H112), S142 (= S140), Y155 (= Y153), K159 (= K157), I200 (≠ Q198)
- binding (3R)-3-hydroxybutanoic acid: Q94 (= Q92), H144 (= H142), K152 (= K150), Y155 (= Y153), W187 (≠ F185), Q196 (= Q194), W257 (= W255)
- binding acetoacetic acid: Q94 (= Q92), H144 (= H142), K152 (= K150), Y155 (= Y153), W187 (≠ F185), Q196 (= Q194), W257 (= W255)
- binding nicotinamide-adenine-dinucleotide: G11 (= G12), T13 (≠ A14), I16 (= I17), F36 (≠ V37), D63 (= D61), L64 (≠ V62), N90 (= N88), A91 (= A89), G92 (= G90), L113 (≠ I111), K159 (= K157), G186 (= G184), V188 (= V186), T190 (= T188), L192 (= L190), V193 (= V191)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G11 (= G12), T13 (≠ A14), I16 (= I17), F36 (≠ V37), D63 (= D61), L64 (≠ V62), N90 (= N88), A91 (= A89), G92 (= G90), L113 (≠ I111), S142 (= S140), Y155 (= Y153), K159 (= K157), G186 (= G184), V188 (= V186), T190 (= T188), L192 (= L190), V193 (= V191)
3vdqA Crystal structure of alcaligenes faecalis d-3-hydroxybutyrate dehydrogenase in complex with NAD(+) and acetate (see paper)
39% identity, 98% coverage: 3:255/258 of query aligns to 2:257/260 of 3vdqA
- active site: G15 (= G16), N114 (≠ H112), S142 (= S140), Y155 (= Y153), K159 (= K157), I200 (≠ Q198)
- binding acetate ion: Q94 (= Q92), H144 (= H142), K152 (= K150), W187 (≠ F185), L192 (= L190), Q196 (= Q194)
- binding nicotinamide-adenine-dinucleotide: G11 (= G12), S14 (≠ G15), I16 (= I17), F36 (≠ V37), D63 (= D61), L64 (≠ V62), N90 (= N88), A91 (= A89), G92 (= G90), L113 (≠ I111), I140 (≠ M138), S142 (= S140), Y155 (= Y153), K159 (= K157), P185 (= P183), G186 (= G184), W187 (≠ F185), V188 (= V186), T190 (= T188), L192 (= L190), V193 (= V191)
2ztlA Closed conformation of d-3-hydroxybutyrate dehydrogenase complexed with NAD+ and l-3-hydroxybutyrate (see paper)
39% identity, 98% coverage: 3:255/258 of query aligns to 2:257/260 of 2ztlA
- active site: G15 (= G16), N114 (≠ H112), S142 (= S140), Y155 (= Y153), K159 (= K157), L200 (≠ Q198)
- binding (3s)-3-hydroxybutanoic acid: Q94 (= Q92), S142 (= S140), H144 (= H142), K152 (= K150), Y155 (= Y153), Q196 (= Q194)
- binding nicotinamide-adenine-dinucleotide: G11 (= G12), G15 (= G16), I16 (= I17), F36 (≠ V37), L64 (≠ V62), N90 (= N88), A91 (= A89), G92 (= G90), L113 (≠ I111), Y155 (= Y153), K159 (= K157), P185 (= P183), W187 (≠ F185), V188 (= V186), T190 (= T188), V193 (= V191)
1wmbA Crystal structure of NAD dependent d-3-hydroxybutylate dehydrogenase (see paper)
39% identity, 98% coverage: 3:255/258 of query aligns to 2:257/260 of 1wmbA
2q2qD Structure of d-3-hydroxybutyrate dehydrogenase from pseudomonas putida (see paper)
38% identity, 98% coverage: 3:256/258 of query aligns to 2:253/255 of 2q2qD
- active site: G15 (= G16), S138 (= S140), Y151 (= Y153), K155 (= K157), R196 (≠ Q198)
- binding nicotinamide-adenine-dinucleotide: G11 (= G12), T13 (≠ A14), S14 (≠ G15), G15 (= G16), I16 (= I17), F36 (vs. gap), D59 (= D61), L60 (≠ V62), N86 (= N88), G88 (= G90), L109 (≠ I111), I136 (≠ M138), S138 (= S140), Y151 (= Y153), K155 (= K157), P181 (= P183), G182 (= G184), W183 (≠ F185), V184 (= V186), T186 (= T188), L188 (= L190), V189 (= V191)
5yssB Crystal structure of aminocaproic acid cyclase in complex with NAD (+) (see paper)
38% identity, 98% coverage: 2:255/258 of query aligns to 1:252/255 of 5yssB
- binding nicotinamide-adenine-dinucleotide: G11 (= G12), T13 (≠ A14), S14 (≠ G15), G15 (= G16), I16 (= I17), G35 (≠ D36), F36 (≠ V37), L60 (≠ T63), N86 (= N88), G88 (= G90), I89 (≠ V91), A137 (≠ G139), Y151 (= Y153), K155 (= K157), P181 (= P183), G182 (= G184), V184 (= V186), T186 (= T188)
1x1tA Crystal structure of d-3-hydroxybutyrate dehydrogenase from pseudomonas fragi complexed with NAD+ (see paper)
38% identity, 98% coverage: 3:255/258 of query aligns to 2:233/236 of 1x1tA
- active site: G15 (= G16), N114 (≠ H112), S142 (= S140), Y155 (= Y153), K159 (= K157)
- binding cacodylate ion: S142 (= S140), H144 (= H142), Y155 (= Y153), W187 (≠ F185), W233 (= W255)
- binding nicotinamide-adenine-dinucleotide: G11 (= G12), T13 (≠ A14), S14 (≠ G15), G15 (= G16), I16 (= I17), G35 (≠ D36), F36 (≠ V37), D63 (= D61), L64 (≠ V62), N90 (= N88), G92 (= G90), L113 (≠ I111), S142 (= S140), Y155 (= Y153), K159 (= K157), P185 (= P183), W187 (≠ F185), V188 (= V186), T190 (= T188)
2cfcA Structural basis for stereo selectivity in the (r)- and (s)- hydroxypropylethane thiosulfonate dehydrogenases (see paper)
35% identity, 97% coverage: 6:255/258 of query aligns to 3:247/250 of 2cfcA
- active site: G13 (= G16), S142 (= S140), Y155 (= Y153), K159 (= K157)
- binding (2-[2-ketopropylthio]ethanesulfonate: F149 (≠ S147), R152 (≠ K150), Y155 (= Y153), W195 (≠ K193), R196 (≠ Q194)
- binding nicotinamide-adenine-dinucleotide: G9 (= G12), S12 (≠ G15), G13 (= G16), N14 (≠ I17), D33 (= D36), L34 (≠ V37), A59 (≠ M60), D60 (= D61), V61 (= V62), N87 (= N88), A88 (= A89), G89 (= G90), I140 (≠ M138), P185 (= P183), G186 (= G184), M187 (≠ F185), I188 (≠ V186), T190 (= T188), P191 (= P189), M192 (≠ L190), T193 (≠ V191)
Q56840 2-(R)-hydroxypropyl-CoM dehydrogenase; R-HPCDH; 2-[(R)-2-hydroxypropylthio]ethanesulfonate dehydrogenase; Aliphatic epoxide carboxylation component III; Epoxide carboxylase component III; RHPCDH1; EC 1.1.1.268 from Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2) (see 4 papers)
35% identity, 97% coverage: 6:255/258 of query aligns to 3:247/250 of Q56840
- SGN 12:14 (≠ GGI 15:17) binding NAD(+)
- D33 (= D36) binding NAD(+)
- DV 60:61 (= DV 61:62) binding NAD(+)
- N87 (= N88) binding NAD(+)
- S142 (= S140) mutation to A: Retains weak activity. 120-fold decrease in kcat.; mutation to C: Loss of activity.
- R152 (≠ K150) binding 2-oxopropyl-coenzyme M; mutation to A: Almost loss of activity with the natural substrate 2-KPC, but does not affect activity with 2-butanone as substrate.
- Y155 (= Y153) mutation Y->E,F: Loss of activity.
- K159 (= K157) mutation to A: Loss of activity.
- R179 (= R177) mutation to A: Loss of activity.
- IETPM 188:192 (≠ VKTPL 186:190) binding NAD(+)
- WR 195:196 (≠ KQ 193:194) binding 2-oxopropyl-coenzyme M
- R196 (≠ Q194) mutation to A: Almost loss of activity with the natural substrate 2-KPC, but does not affect activity with 2-butanone as substrate.
- R203 (= R201) mutation to A: Slight decrease in catalytic efficiency.
- R209 (= R212) mutation to A: Does not affect catalytic efficiency.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
6ixmC Crystal structure of the ketone reductase chkred20 from the genome of chryseobacterium sp. Ca49 complexed with NAD (see paper)
33% identity, 98% coverage: 3:255/258 of query aligns to 3:245/248 of 6ixmC
- active site: G16 (= G16), S142 (= S140), Y155 (= Y153), K159 (= K157)
- binding nicotinamide-adenine-dinucleotide: G12 (= G12), S15 (≠ G15), G16 (= G16), I17 (= I17), D36 (= D36), I37 (≠ V37), A61 (≠ M60), D62 (= D61), T63 (≠ V62), N89 (= N88), A90 (= A89), M140 (= M138), S142 (= S140), Y155 (= Y153), K159 (= K157), P185 (= P183), A186 (≠ G184), Y187 (≠ F185), I188 (≠ V186), L192 (= L190)
F1SWA0 Zerumbone synthase; EC 1.1.1.326 from Zingiber zerumbet (Shampoo ginger) (Amomum zerumbet) (see paper)
33% identity, 99% coverage: 1:255/258 of query aligns to 1:256/267 of F1SWA0
- S142 (= S140) mutation to A: Strong reduction in oxidoreductase activity toward 8-hydroxy-alpha-humulene and borneol.
- S144 (≠ H142) mutation to A: Increased oxidoreductase activity toward 8-hydroxy-alpha-humulene and borneol.
- Y155 (= Y153) mutation to A: Strong reduction in oxidoreductase activity toward 8-hydroxy-alpha-humulene and borneol.
- K159 (= K157) mutation to A: Abolishes all oxidoreductase activity.
1zk1A Structure of r-specific alcohol dehydrogenase (mutant g37d) from lactobacillus brevis in complex with phenylethanol and NAD (see paper)
33% identity, 98% coverage: 2:255/258 of query aligns to 3:248/251 of 1zk1A
- active site: G17 (= G16), S142 (= S140), Y155 (= Y153), K159 (= K157)
- binding 1-phenylethanone: A93 (≠ Q92), N95 (≠ V94), Y155 (= Y153), Y189 (≠ F185)
- binding nicotinamide-adenine-dinucleotide: G13 (= G12), L16 (≠ G15), I18 (= I17), D37 (= D36), H61 (≠ M60), D62 (= D61), S63 (≠ V62), N89 (= N88), A90 (= A89), I92 (≠ V91), M140 (= M138), Y155 (= Y153), G188 (= G184), I190 (≠ V186), L194 (= L190)
1zjzA Structure of r-specific alcohol dehydrogenase (mutant g37d) from lactobacillus brevis in complex with phenylethanol and NAD (see paper)
33% identity, 98% coverage: 2:255/258 of query aligns to 3:248/251 of 1zjzA
- active site: G17 (= G16), S142 (= S140), Y155 (= Y153), K159 (= K157)
- binding nicotinamide-adenine-dinucleotide: G13 (= G12), L16 (≠ G15), I18 (= I17), D37 (= D36), D62 (= D61), N89 (= N88), A90 (= A89), G91 (= G90), I92 (≠ V91), Y155 (= Y153), G188 (= G184), I190 (≠ V186), L194 (= L190)
- binding (1r)-1-phenylethanol: A93 (≠ Q92), N95 (≠ V94), L152 (≠ K150), Y155 (= Y153)
Query Sequence
>WP_013417865.1 NCBI__GCF_000166055.1:WP_013417865.1
MELDGKIAIVTGAAGGIGLAIAKRYVDAGARVAIADVRLDAAEQAARSLGGPDKAIALEM
DVTSEDAVNAGVAKTVETFGTVDILVSNAGVQIVHPVEDFPYADWKKMLAIHLDGAFLTS
KACLPHMYKQNSGTIIFMGSVHSKAPSALKSAYVTAKHGLQGLARVIAMEGAGHGVRTNV
ICPGFVKTPLVEKQIPEQAARLNISEKEVVERIMLGQTVDKEFTTMEDVAEVALFFAAFP
TNALTGQSLVVSHGWFMN
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory