SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013418144.1 NCBI__GCF_000166055.1:WP_013418144.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 8 hits to proteins with known functional sites (download)

1um0A Crystal structure of chorismate synthase complexed with fmn (see paper)
45% identity, 97% coverage: 4:354/362 of query aligns to 2:348/365 of 1um0A

query
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1um0A

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binding flavin mononucleotide: H104 (= H111), R123 (= R130), S125 (= S132), N241 (= N242), G242 (≠ A243), L280 (= L286), G281 (= G287), K296 (= K302), T298 (= T304), P299 (≠ S305), S300 (= S306), I327 (≠ V333), R330 (= R336)

P56122 Chorismate synthase; CS; 5-enolpyruvylshikimate-3-phosphate phospholyase; EC 4.2.3.5 from Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) (see paper)
45% identity, 97% coverage: 4:354/362 of query aligns to 2:348/365 of P56122

query
sites
P56122

N

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RSS 123:125 (= RSS 130:132) binding FMN
KPTPS 296:300 (≠ KPTSS 302:306) binding FMN

Q12640 Chorismate synthase aro-2; 5-enolpyruvylshikimate-3-phosphate phospholyase; EC 1.5.1.38; EC 4.2.3.5 from Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) (see 3 papers)
41% identity, 98% coverage: 4:359/362 of query aligns to 2:396/432 of Q12640

query
sites
Q12640

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S16 (= S18) mutation to A: Does not affect the flavin reductase activity but reduces the chorismate synthase activity 70-fold.
H17 (= H19) active site; mutation to A: Reduces the chorismate synthase activity 20-fold.
H106 (= H111) active site; mutation to A: Reduces the chorismate synthase activity 10-fold.
S127 (= S132) mutation to A: Does not affect the flavin reductase activity but reduces the chorismate synthase activity 6-fold.
D367 (= D330) active site; mutation D->A,N: Does not affect substrate binding but stringly reduces the chorismate synthase activity.

2qhfA Mycobacterium tuberculosis chorismate synthase in complex with nca
38% identity, 94% coverage: 9:350/362 of query aligns to 1:363/392 of 2qhfA

query
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2qhfA

M

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-

A

-

R

-

N

-

A

-

P

-

L

-

T

K

R

F

P

R

R

P

P

G

G

H

H

A

A

D

D

F

Y

T

A

Y

G

V

M

E

L

K

K

Y

Y

G

G

I

F

R

D

D

D

Y

A

R

R

G

P

G

V

-

L

G

E

R

R

S

A

S

S

A
|
A

R

R

E

E

T

T

A

A

M

A

R

R

V

V

A

A

A

A

G

G

A

T

V

V

A

A

R

R

K

A

V

F

V

L

-

R

-

Q

-

A

P

L

G

G

I

V

A

E

I

V

R

L

G

S

A

H

L

V

V

I

Q

S

V

I

G

G

A

A

E

S

Q

A

I

P

-

Y

-

E

-

G

-

P

-

P

D

P

R

R

A

A

R

E

W

-

D

D

W

L

A

P

E

A

V

I

E

D

R

A

N

S

P

P

F

V

F

R

A

A

P

Y

D

D

A

K

G

A

I

A

V

E

E

A

R

D

W

M

T

I

A

A

Y

Q

L

I

D

E

G

A

V

A

R

K

K

K

A

D

G

G

S

D

S

T

C

L

G

G

A

G

V

V

V

V

E

E

V

A

V

V

A

A

E

L

G

G

A

L

P

P

A

V

G

G

L

L

G

G

A

S

P

F

L

T

Y

S

G

G

-

D

-

H

K

R

L

L

D

D

Q

S

D

Q

I

L

A

A

A

A

A

A

F

V

M

M

S

G

I
|
I

N
x
Q

A

A

V

I

K

K

G

G

V

V

E

E

I

I

G

G

E

D

G

G

F

F

A

Q

S

T

A

A

A

R

L

R

S

R

G

G

E

S

D

R

N

A

A

H

D

D

E

E

M

M

R

Y

I

P

G

G

P

P

D

D

G

G

Q

V

P

-

V

V

F

R

L

S

S

T

N

N

H

R

A

A

G

G

G

G

I

L

L

E

G

G

G

G

I

M

S

T

T

N

G

G

Q

Q

P

P

I

L

V

R

A

V

R

R

F

A

A

A

V
x
M

K

K

P

P

T
x
I

S

S

S

T

I

V

L

P

T

R

P

A

R

L

R

A

T

T

I

V

D

D

T

L

Q

A

G

T

H

G

D

D

A

E

E

A

I

V

L

A

T

I

K

H

G

Q

R
|
R

H

S

D

D

P

V

C

C

V

A

G

V

I

P

R

A

A

A

V

G

P

V

V

V

G

V

E

E

A

T

M

M

M

V

A

A

I

L

V

V

L

L

A

A

binding nicotinamide: R46 (= R54), A138 (= A133), I255 (= I241), Q256 (≠ N242), M314 (≠ V301), I317 (≠ T304), R341 (= R328)

P9WPY1 Chorismate synthase; CS; 5-enolpyruvylshikimate-3-phosphate phospholyase; EC 4.2.3.5 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
38% identity, 94% coverage: 9:350/362 of query aligns to 1:363/401 of P9WPY1

query
sites
P9WPY1

M

M

F

L

R

R

V

W

T

I

T

T

W

A

G

G

E

E

S

S

H

H

G

G

P

R

A

A

I

L

G

V

C

A

V

V

I

V

D

E

G

G

C

M

P

V

P

A

G

G

I

V

P

H

L

V

T

T

E

S

A

A

D

D

I

I

Q

A

P

D

F

Q

L

L

D

A

K

R

R
|
R

R

R

P

L

G

G

Q

Y

S

G

R
|
R

F

G

T

A

T

R

Q

M

R

T

Q

F

E

E

E

R

D

D

R

A

V

V

R

T

I

V

L

L

S

S

G

G

V

I

F

R

P

H

N

G

E

S

A

-

G

-

V

-

Q

-

V

-

T

T

T

L

G

G

T

G

P

P

V

I

A

A

L

I

E

E

I

I

Q

G

N

N

T

T

D

E

-

W

-

P

-

K

-

W

-

E

-

T

-

V

-

M

-

A

Q

A

R

D

S

P

K

V

D

D

Y

P

S

A

D

E

I

L

K

A

D

D

-

V

-

A

-

R

-

N

-

A

-

P

-

L

-

T

K

R

F

P

R

R

P

P

G

G

H

H

A

A

D

D

F

Y

T

A

Y

G

V

M

E

L

K

K

Y

Y

G

G

I

F

R

D

D

D

Y

A

R

R

G

P

G

V

-

L

G

E

R

R

S

A

S

S

A

A

R

R

E

E

T

T

A

A

M

A

R

R

V

V

A

A

A

A

G

G

A

T

V

V

A

A

R

R

K

A

V

F

V

L

-

R

-

Q

-

A

P

L

G

G

I

V

A

E

I

V

R

L

G

S

A

H

L

V

V

I

Q

S

V

I

G

G

A

A

E

S

Q

A

I

P

-

Y

-

E

-

G

-

P

-

P

D

P

R

R

A

A

R

E

W

-

D

D

W

L

A

P

E

A

V

I

E

D

R

A

N

S

P

P

F

V

F

R

A

A

P

Y

D

D

A

K

G

A

I

A

V

E

E

A

R

D

W

M

T

I

A

A

Y

Q

L

I

D

E

G

A

V

A

R

K

K

K

A

D

G

G

S

D

S

T

C

L

G

G

A

G

V

V

V

V

E

E

V

A

V

V

A

A

E

L

G

G

A

L

P

P

A

V

G

G

L

L

G

G

A

S

P

F

L

T

Y

S

G

G

-

D

-

H

K

R

L

L

D

D

Q

S

D

Q

I

L

A

A

A

A

A

A

F

V

M

M

S

G

I

I

N
x
Q

A
|
A

V

I

K

K

G

G

V

V

E

E

I

I

G

G

E

D

G

G

F

F

A

Q

S

T

A

A

A

R

L

R

S

R

G

G

E

S

D

R

N

A

A

H

D

D

E

E

M

M

R

Y

I

P

G

G

P

P

D

D

G

G

Q

V

P

-

V

V

F

R

L

S

S

T

N

N

H

R

A

A

G

G

G

G

I

L

L

E

G

G

G

G

I

M

S

T

T

N

G

G

Q

Q

P

P

I

L

V

R

A

V

R

R

F

A

A

A

V

M

K
|
K

P
|
P

T
x
I

S
|
S

S
x
T

I

V

L

P

T

R

P

A

R

L

R

A

T

T

I

V

D

D

T

L

Q

A

G

T

H

G

D

D

A

E

E

A

I

V

L

A

T

I

K

H

G

Q

R
|
R

H

S

D

D

P

V

C

C

V

A

G

V

I

P

R

A

A

A

V

G

P

V

V

V

G

V

E

E

A

T

M

M

M

V

A

A

I

L

V

V

L

L

A

A

R40 (= R48) binding NADP(+)
R46 (= R54) binding NADP(+)
QA 256:257 (≠ NA 242:243) binding FMN
KPIST 315:319 (≠ KPTSS 302:306) binding FMN
R341 (= R328) binding FMN

2o12A Mycobacterium tuberculosis chorismate synthase in complex with fmn
38% identity, 94% coverage: 9:350/362 of query aligns to 1:357/386 of 2o12A

query
sites
2o12A

M

M

F

L

R

R

V

W

T

I

T

T

W

A

G

G

E

E

S

S

H

H

G

G

P

R

A

A

I

L

G

V

C

A

V

V

I

V

D

E

G

G

C

M

P

V

P

A

G

G

I

V

P

H

L

V

T

T

E

S

A

A

D

D

I

I

Q

A

P

D

F

Q

L

L

D

A

K

R

R

R

R

R

P

L

G

G

Q

Y

S

G

R

R

F

-

T

-

T

-

Q

-

R

-

Q

-

E

E

E

R

D

D

R

A

V

V

R

T

I

V

L

L

S

S

G

G

V

I

F

R

P

H

N

G

E

S

A

-

G

-

V

-

Q

-

V

-

T

T

T

L

G

G

T

G

P

P

V

I

A

A

L

I

E

E

I

I

Q

G

N

N

T

T

D

E

-

W

-

P

-

K

-

W

-

E

-

T

-

V

-

M

-

A

Q

A

R

D

S

P

K

V

D

D

Y

P

S

A

D

E

I

L

K

A

D

D

-

V

-

A

-

R

-

N

-

A

-

P

-

L

-

T

K

R

F

P

R

R

P

P

G

G

H
|
H

A

A

D

D

F

Y

T

A

Y

G

V

M

E

L

K

K

Y

Y

G

G

I

F

R

D

D

D

Y

A

R

R

G

P

G

V

-

L

G

E

R

R

S

A

S

S

A
|
A

R

R

E

E

T

T

A

A

M

A

R

R

V

V

A

A

A

A

G

G

A

T

V

V

A

A

R

R

K

A

V

F

V

L

-

R

-

Q

-

A

P

L

G

G

I

V

A

E

I

V

R

L

G

S

A

H

L

V

V

I

Q

S

V

I

G

G

A

A

E

S

Q

A

I

P

-

Y

-

E

-

G

-

P

-

P

D

P

R

R

A

A

R

E

W

-

D

D

W

L

A

P

E

A

V

I

E

D

R

A

N

S

P

P

F

V

F

R

A

A

P

Y

D

D

A

K

G

A

I

A

V

E

E

A

R

D

W

M

T

I

A

A

Y

Q

L

I

D

E

G

A

V

A

R

K

K

K

A

D

G

G

S

D

S

T

C

L

G

G

A

G

V

V

V

V

E

E

V

A

V

V

A

A

E

L

G

G

A

L

P

P

A

V

G

G

L

L

G

G

A

S

P

F

L

T

Y

S

G

G

-

D

-

H

K

R

L

L

D

D

Q

S

D

Q

I

L

A

A

A

A

A

A

F

V

M

M

S

G

I

I

N
x
Q

A
|
A

V

I

K

K

G

G

V

V

E

E

I

I

G

G

E

D

G

G

F

F

A

Q

S

T

A

A

A

R

L

R

S

R

G

G

E

S

D

R

N

A

A

H

D

D

E

E

M

M

R

Y

I

P

G

G

P

P

D

D

G

G

Q

V

P

-

V

V

F

R

L

S

S

T

N

N

H

R

A

A

G

G

G

G

I

L

L

E

G

G

G

G

I

M

S

T

T

N

G

G

Q

Q

P

P

I

L

V

R

A

V

R

R

F

A

A

A

V
x
M

K
|
K

P

P

T
x
I

S

S

S
x
T

I

V

L

P

T

R

P

A

R

L

R

A

T

T

I

V

D

D

T

L

Q

A

G

T

H

G

D

D

A

E

E

A

I

V

L

A

T

I

K

H

G

Q

R
|
R

H

S

D

D

P

V

C

C

V

A

G

V

I

P

R

A

A

A

V

G

P

V

V

V

G

V

E

E

A

T

M

M

M

V

A

A

I

L

V

V

L

L

A

A

binding flavin mononucleotide: H109 (= H111), A132 (= A133), Q250 (≠ N242), A251 (= A243), M308 (≠ V301), K309 (= K302), I311 (≠ T304), T313 (≠ S306), R335 (= R328)

1qxoA Crystal structure of chorismate synthase complexed with oxidized fmn and epsp (see paper)
36% identity, 95% coverage: 10:354/362 of query aligns to 1:363/388 of 1qxoA

query
sites
1qxoA

F

M

R

R

V

Y

T

L

T

T

W

A

G

G

E

E

S

S

H

H

G

G

P

P

A

R

I

L

G

T

C

A

V

I

I

I

D

E

G

G

C

I

P

P

P

A

G

G

I

L

P

P

L

L

T

T

E

A

A

E

D

D

I

I

Q

N

P

E

F

D

L

L

D

R

K

R

R
|
R

R

Q

P

G

G

G

Q

Y

S

G

R
|
R

F

G

T

G

T
x
R

Q
x
M

R

K

Q

I

E

E

E

N

D

D

R

Q

V

V

R

V

I

F

L

T

S

S

G

G

V

V

F

R

P

H

N

G

E

K

A

-

G

-

V

-

Q

-

V

-

T

T

T

T

G

G

T

A

P

P

V

I

A

T

L

M

E

D

I

V

Q

I

N

N

T

K

D

D

Q

H

R

Q

S

K

-

W

-

L

-

D

-

I

K

M

D

S

Y

A

S

E

D

D

I

I

K

E

D

D

K

R

F

L

-

K

-

S

-

K

-

R

-

K

-

I

-

T

-

H

-

P

R

R

P

P

G

G

H
|
H

A

A

D

D

F

L

T

V

Y

G

V

G

E

I

K

K

Y

Y

G

R

I

F

R

D

D

D

Y

L

R

R

G

N

G

S

-

L

G

E

R

R

S

S

S

S

A
|
A

R
|
R

E

E

T

T

A

T

M

M

R

R

V

V

A

A

A

V

G

G

A

A

V

V

A

A

R

K

K

R

V

L

V

L

P

A

-

E

-

L

G

D

I

M

A

E

I

I

R

A

G

N

A

H

L

V

V

V

Q

V

V

F

G

G

A

G

E

K

Q

E

I

I

D

D

R

V

-

P

A

E

R

N

W

L

D

T

W

V

A

A

E

E

V

I

E

K

R

Q

N

R

P

A

F

A

F

-

A

Q

P

S

D

E

A

V

G

S

I

I

V

V

-

N

-

Q

-

E

-

R

-

E

E

Q

R

E

W

I

T

K

A

D

Y

Y

L

I

D

D

G

Q

V

I

R

K

K

R

A

D

G

G

S

D

S

T

C

I

G

G

A

G

V

V

V

V

E

E

V

T

V

V

A

V

E

G

G

G

A

V

P

P

A

V

G

G

L

L

G

G

A

S

P

Y

L

V

Y

Q

-

W

-

D

G

R

K

K

L

L

D

D

Q

A

D

R

I

L

A

A

A

Q

A

A

F

V

M

V

S

S

I

I

N
|
N

A
|
A

V

F

K

K

G

G

V

V

E

E

I

F

G

G

E

L

G

G

F

F

A

E

S

A

A

G

A

Y

L

R

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G

G

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N

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D

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K

D

E

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G

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F

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N

N

H

N

A

L

G

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G

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G

G

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G

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x
M

K
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K

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x
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x
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x
T

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binding 5-[(1-carboxyvinyl)oxy]-4-hydroxy-3-(phosphonooxy)cyclohex-1-ene-1-carboxylic acid: R39 (= R48), R45 (= R54), R48 (≠ T57), M49 (≠ Q58), A133 (= A133), R134 (= R134)
binding flavin mononucleotide: H110 (= H111), A133 (= A133), N251 (= N242), A252 (= A243), M310 (≠ V301), K311 (= K302), I313 (≠ T304), P314 (≠ S305), T315 (≠ S306)

P0A2Y6 Chorismate synthase; CS; 5-enolpyruvylshikimate-3-phosphate phospholyase; EC 4.2.3.5 from Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) (see paper)
36% identity, 95% coverage: 10:354/362 of query aligns to 1:363/388 of P0A2Y6

query
sites
P0A2Y6

F

M

R

R

V

Y

T

L

T

T

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A

G

G

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E

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C

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G

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T

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A

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D

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F

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K

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S

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N

G

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K

A

-

G

-

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Q

-

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-

T

T

T

T

G

G

T

A

P

P

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I

A

T

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D

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N

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K

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K

-

W

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I

K

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D

D

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-

K

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K

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K

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H

A

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K

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A

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A

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-

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-

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Y

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N
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N

A
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A

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K

G

G

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V

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E

I

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N

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G

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G
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G

G

G

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G

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Q

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NA 251:252 (= NA 242:243) binding FMN
G296 (= G287) binding FMN
KPIPT 311:315 (≠ KPTSS 302:306) binding FMN

Query Sequence

>WP_013418144.1 NCBI__GCF_000166055.1:WP_013418144.1
MSYNTFGHMFRVTTWGESHGPAIGCVIDGCPPGIPLTEADIQPFLDKRRPGQSRFTTQRQ
EEDRVRILSGVFPNEAGVQVTTGTPVALEIQNTDQRSKDYSDIKDKFRPGHADFTYVEKY
GIRDYRGGGRSSARETAMRVAAGAVARKVVPGIAIRGALVQVGAEQIDRARWDWAEVERN
PFFAPDAGIVERWTAYLDGVRKAGSSCGAVVEVVAEGAPAGLGAPLYGKLDQDIAAAFMS
INAVKGVEIGEGFASAALSGEDNADEMRIGPDGQPVFLSNHAGGILGGISTGQPIVARFA
VKPTSSILTPRRTIDTQGHDAEILTKGRHDPCVGIRAVPVGEAMMAIVLADHWLRHRAQT
GR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory