SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013419358.1 NCBI__GCF_000166055.1:WP_013419358.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0A717 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Escherichia coli (strain K12) (see 4 papers)
56% identity, 100% coverage: 1:310/310 of query aligns to 4:314/315 of P0A717

query
sites
P0A717

M

M

K

K

L

L

V

F

A

A

G

G

N

N

S

A

N

T

R

P

P

E

L

L

A

A

N

Q

A

R

I

I

A

A

A

N

Y

R

L

L

K

Y

T

T

P

S

L

L

T

G

E

D

C

A

L

A

V

V

R

G

R

R

F

F

S

S

D

D

M

G

E

E

I

V

F

S

V

V

E

Q

V

I

R

N

E

E

N

N

V

V

R

R

G

G

E

G

D

D

I

I

F

F

V

I

I

I

Q

Q

S

S

T

T

S

C

Y

A

P

P

A

T

N

N

D

D

N

N

L

L

M

M

E

E

L

L

L

V

I

V

L

M

T

V

D

D

A

A

L

L

R

R

A

A

S

S

A

A

R

G

R

R

I

I

T

T

A

A

V

V

I

I

P

P

Y

Y

F

F

G

G

Y

Y

A

A

R

R

Q

Q

D

D

R

R

K

R

T

V

L

R

P

S

-

A

R

R

T

V

P

P

I

I

S

T

A

A

K

K

L

V

V

V

A

A

N

D

L

F

I

L

T

S

H

S

A

V

G

G

A

V

D

D

R

R

V

V

L

L

T

T

V

V

D
|
D

L

L

H

H

A

A

G

E

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

V

P

P

T

V

D

D

N

N

L

V

Y

F

A

G

A

S

P

P

V

I

M

L

V

L

R

E

D

D

I

M

T

L

K

Q

R

L

Y

N

T

L

D

D

G

-

N

N

I

P

T

I

V

V

S

S

P

P

D

D

V

I

G

G

V

V

R

R

A

A

R

R

S

A

L

I

A

A

S

K

R

L

I

L

-

N

D

D

A

T

P

D

L

M

A

A

I

I

D

D

K

K

R

R

D

P

R

R

P

A

G

N

E

V

S

S

E

Q

V

V

M

M

N

H

V

I

I

I

G

G

N

D

V

V

T

A

G

G

R

R

H

D

C

C

I

V

L

L

I

V

D
|
D

I

M

I

I

D
|
D

S

T

G

G

T

T

L

L

C

C

N

K

A

A

H

E

A

A

L

L

I

K

E

E

K

R

G

G

A

A

C

K

S

R

V

V

A

F

A

A

Y

Y

A

A

T

T

H

H

G

P

V

I

F

F

S

S

G

G

G

N

A

A

V

A

A

N

R

N

I

L

Q

R

A

N

S

S

K

V

I

I

D

D

T

E

L

V

V

V

I

V

T

C

D

D

T

T

I

I

V

P

P

L

T

S

E

D

A

E

V

I

R

K

V

S

A

L

S

P

N

N

I

V

E

R

V

T

L

L

S

T

L

L

S

S

A

G

L

M

I

L

G

A

E

E

A

A

I

I

L

R

R

R

T

I

S

S

G

N

E

E

S

S

V

I

S

S

S

A

L

M

F

F

D

E

D129 (= D125) to A: in mutant PRSA1; alters the binding of divalent cations, especially magnesium. Little alteration in the affinity for ribose 5-phosphate and 27-fold decrease of the affinity for ATP. Absence of inhibition by AMP
D220 (= D216) mutation to E: 4-fold decrease in the affinity binding for Rib-5-P in the presence of magnesium ions. In the presence of cobalt ions, it shows a 15-fold decrease in the affinity binding for Rib-5-P.; mutation to F: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.
D221 (= D217) mutation to A: The affinity binding for ATP is comparable to those of the wild-type, apart from a slight decrease in the presence of manganese ions. The affinity binding for Rib-5-P is greatly decreased in the presence of both manganese and cobalt ions but only about 2-fold in the presence of magnesium ions.
D224 (= D220) mutation to A: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.; mutation to S: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

6asvC E. Coli prpp synthetase (see paper)
56% identity, 100% coverage: 1:309/310 of query aligns to 2:311/311 of 6asvC

query
sites
6asvC

M

M

K

K

L

L

V

F

A

A

G

G

N

N

S

A

N

T

R

P

P

E

L

L

A

A

N

Q

A

R

I

I

A

A

A

N

Y

R

L

L

K

Y

T

T

P

S

L

L

T

G

E

D

C

A

L

A

V

V

R

G

R

R

F

F

S

S

D

D

M

G

E

E

I

V

F

S

V

V

E

Q

V

I

R

N

E

E

N

N

V

V

R

R

G

G

E

G

D

D

I

I

F

F

V

I

I

I

Q

Q

S

S

T

T

S

C

Y

A

P

P

A

T

N

N

D

D

N

N

L

L

M

M

E

E

L

L

L

V

I

V

L

M

T

V

D

D

A

A

L

L

R

R

A

A

S

S

A

A

R

G

R

R

I

I

T

T

A

A

V

V

I

I

P

P

Y

Y

F

F

G

G

Y

Y

A

A

R

R

Q

Q

D

D

R

R

K

R

T

V

L

R

P

S

-

A

R

R

T

V

P

P

I

I

S

T

A

A

K

K

L

V

V

V

A

A

N

D

L

F

I

L

T

S

H

S

A

V

G

G

A

V

D

D

R

R

V

V

L

L

T

T

V

V

D

D

L

L

H

H

A

A

G

E

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

V

P

P

T

V

D

D

N

N

L

V

Y

F

A

G

A

S

P

P

V

I

M

L

V

L

R

E

D

D

I

M

T

L

K

Q

R

L

Y

N

T

L

D

D

G

-

N

N

I

P

T

I

V

V

S

S

P

P

D
|
D

V

I

G

G

V

V

R

R

A

A

R

R

S

A

L

I

A

A

S

K

R

L

I

L

-

N

D

D

A

T

P

D

L

M

A

A

I

I

D

D

K

K

R

R

D

P

R

R

P

A

G

N

E

V

S

S

E

Q

V

V

M

M

N

H

V

I

I

I

G

G

N

D

V

V

T

A

G

G

R

R

H

D

C

C

I

V

L

L

I

V

D
|
D

I

M

I

I

D
|
D

S
x
T

G
|
G

G

G

T
|
T

L
|
L

C

C

N

K

A

A

H

E

A

A

L

L

I

K

E

E

K

R

G

G

A

A

C

K

S

R

V

V

A

F

A

A

Y

Y

A

A

T

T

H

H

G

P

V

I

F

F

S

S

G

G

G

N

A

A

V

A

A

N

R

N

I

L

Q

R

A

N

S

S

K

V

I

I

D

D

T

E

L

V

V

V

I

V

T

C

D

D

T

T

I

I

V

P

P

L

T

S

E

D

A

E

V

I

R

K

V

S

A

L

S

P

N

N

I

V

E

R

V

T

L

L

S

T

L

L

S

S

A

G

L

M

I

L

G

A

E

E

A

A

I

I

L

R

R

R

T

I

S

S

G

N

E

E

S

S

V

I

S

S

S

A

L

M

F

F

binding phosphate ion: D168 (= D167), D218 (= D216), D219 (= D217), D222 (= D220), T223 (≠ S221), G224 (= G222), T226 (= T224), L227 (= L225)

4s2uA Crystal structure of the phosphorybosylpyrophosphate synthetase from e. Coli
56% identity, 98% coverage: 1:305/310 of query aligns to 3:308/308 of 4s2uA

query
sites
4s2uA

M

M

K

K

L

L

V

F

A

A

G

G

N

N

S

A

N

T

R

P

P

E

L

L

A

A

N

Q

A

R

I

I

A

A

A

N

Y

R

L

L

K

Y

T

T

P

S

L

L

T

G

E

D

C

A

L

A

V

V

R

G

R

R

F

F

S

S

D

D

M

G

E

E

I

V

F

S

V

V

E

Q

V

I

R

N

E

E

N

N

V

V

R

R

G

G

E

G

D

D

I

I

F

F

V

I

I

I

Q

Q

S

S

T

T

S

C

Y

A

P

P

A

T

N

N

D

D

N

N

L

L

M

M

E

E

L

L

L

V

I

V

L

M

T

V

D

D

A

A

L

L

R

R

A

A

S

S

A

A

R

G

R

R

I

I

T

T

A

A

V

V

I

I

P

P

Y

Y

F

F

G

G

Y

Y

A

A

R

R

Q

Q

D

D

R

R

K

R

T

V

L

R

P

S

-

A

R

R

T

V

P

P

I

I

S

T

A

A

K

K

L

V

V

V

A

A

N

D

L

F

I

L

T

S

H

S

A

V

G

G

A

V

D

D

R

R

V

V

L

L

T

T

V

V

D

D

L

L

H

H

A

A

G

E

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

V

P

P

T

V

D

D

N

N

L

V

Y

F

A

G

A

S

P

P

V

I

M

L

V

L

R

E

D

D

I

M

T

L

K

Q

R

L

Y

N

T

L

D

D

G

-

N

N

I

P

T

I

V

V

S

S

P

P

D

D

V

I

G

G

V

V

R

R

A

A

R

R

S

A

L

I

A

A

S

K

R

L

I

L

-

N

D

D

A

T

P

D

L

M

A

A

I

I

D

D

K

K

R

R

D

P

R

R

P

A

G

N

E

V

S

S

E

Q

V

V

M

M

N

H

V

I

I

I

G

G

N

D

V

V

T

A

G

G

R

R

H

D

C

C

I

V

L

L

I

V

D

D

I

M

I

I

D
|
D

S

T

G

G

T
|
T

L

L

C

C

N

K

A

A

H

E

A

A

L

L

I

K

E

E

K

R

G

G

A

A

C

K

S

R

V

V

A

F

A

A

Y

Y

A

A

T

T

H

H

G

P

V

I

F

F

S

S

G

G

G

N

A

A

V

A

A

N

R

N

I

L

Q

R

A

N

S

S

K

V

I

I

D

D

T

E

L

V

V

V

I

V

T

C

D

D

T

T

I

I

V

P

P

L

T

S

E

D

A

E

V

I

R

K

V

S

A

L

S

P

N

N

I

V

E

R

V

T

L

L

S

T

L

L

S

S

A

G

L

M

I

L

G

A

E

E

A

A

I

I

L

R

R

R

T

I

S

S

G

N

E

E

S

S

V

I

binding magnesium ion: D223 (= D220), T227 (= T224)

7xmvA E.Coli phosphoribosylpyrophosphate (prpp) synthetase type a(amp/adp) filament bound with adp, amp and r5p (see paper)
55% identity, 100% coverage: 1:310/310 of query aligns to 2:306/307 of 7xmvA

query
sites
7xmvA

M

M

K

K

L

L

V

F

A

A

G

G

N

N

S

A

N

T

R

P

P

E

L

L

A

A

N

Q

A

R

I

I

A

A

A

N

Y

R

L

L

K

Y

T

T

P

S

L

L

T

G

E

D

C

A

L

A

V

V

R

G

R

R

F
|
F

S

S

D
|
D

M

G

E
|
E

I

V

F

S

V

V

E

Q

V

I

R

N

E

E

N

N

V

V

R

R

G

G

E

G

D

D

I

I

F

F

V

I

I

I

Q

Q

S

S

T

T

S

C

Y

A

P

P

A

T

N

N

D

D

N

N

L

L

M

M

E

E

L

L

L

V

I

V

L

M

T

V

D

D

A

A

L

L

R

R

A

A

S

S

A

A

R

G

R

R

I

I

T

T

A

A

V

V

I

I

P

P

Y

Y

F

F

G

G

Y

Y

A

A

R
|
R

Q

Q

D

D

R
|
R

K

R

T
x
V

L
x
R

P

S

-

A

R

R

T

V

P

P

I

I

S

T

A

A

K

K

L

V

V

V

A

A

N

D

L

F

I

L

T

S

H

S

A

V

G

G

A

V

D

D

R

R

V

V

L

L

T

T

V

V

D

D

L

L

H
|
H

A

A

G
x
E

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

V

P

P

T

V

D

D

N

N

L

V

Y
x
F

A

G

A
x
S

P

P

V

I

M

L

V

L

R

E

D

D

I

M

T

L

K

Q

R

L

Y

N

T

L

D

D

G

-

N

N

I

P

T

I

V

V

S

S

P

P

D

D

V

I

G

G

V

V

V
|
V

R

R

A

A

R

R

S
x
A

L

I

A

A

S

K

R

L

I

L

-

N

D

D

A

T

P

D

L

M

A

A

I

I

D

D

K

K

R

R

D

-

R

-

P

-

G

-

E

-

S

-

E

Q

V

V

M

M

N

H

V

I

I

I

G

G

N

D

V

V

T

A

G

G

R

R

H

D

C

C

I

V

L

L

I

V

D
|
D

I
x
M

I

I

D
|
D

S
x
T

G

G

G
|
G

T
|
T

L

L

C

C

N

K

A

A

H

E

A

A

L

L

I

K

E

E

K

R

G

G

A

A

C

K

S

R

V

V

A

F

A

A

Y

Y

A

A

T

T

H

H

G

P

V

I

F

F

S

S

G

G

G

N

A

A

V

A

A

N

R

N

I

L

Q

R

A

N

S

S

K

V

I

I

D

D

T

E

L

V

V

V

I

V

T

C

D

D

T

T

I

I

V

P

P

L

T

S

E

D

A

E

V

I

R

K

V

S

A

L

S

P

N

N

I

V

E

R

V

T

L

L

S

T

L

L

S

S

A

G

L

M

I

L

G

A

E

E

A

A

I

I

L

R

R

R

T

I

S

S

G

N

E

E

S

S

V

I

S

S

S

A

L

M

F

F

D

E

binding adenosine-5'-diphosphate: F33 (= F32), D35 (= D34), E37 (= E36), R94 (= R93), R97 (= R96), H129 (= H127)
binding adenosine monophosphate: R97 (= R96), V99 (≠ T98), R100 (≠ L99), E131 (≠ G129), F145 (≠ Y143), S147 (≠ A145), V173 (= V172), A177 (≠ S176)
binding 5-O-phosphono-alpha-D-ribofuranose: D212 (= D216), D213 (= D217), M214 (≠ I218), D216 (= D220), T217 (≠ S221), G219 (= G223), T220 (= T224)

7xmuA E.Coli phosphoribosylpyrophosphate (prpp) synthetase type a filament bound with adp, pi and r5p (see paper)
55% identity, 100% coverage: 1:310/310 of query aligns to 2:306/307 of 7xmuA

query
sites
7xmuA

M

M

K

K

L

L

V

F

A

A

G

G

N

N

S

A

N

T

R

P

P

E

L

L

A

A

N

Q

A

R

I

I

A

A

A

N

Y

R

L

L

K

Y

T

T

P

S

L

L

T

G

E

D

C

A

L

A

V

V

R

G

R

R

F
|
F

S

S

D
|
D

M

G

E
|
E

I

V

F

S

V

V

E

Q

V

I

R

N

E

E

N

N

V

V

R

R

G

G

E

G

D

D

I

I

F

F

V

I

I

I

Q

Q

S

S

T

T

S

C

Y

A

P

P

A

T

N

N

D

D

N

N

L

L

M

M

E

E

L

L

L

V

I

V

L

M

T

V

D

D

A

A

L

L

R

R

A

A

S

S

A

A

R

G

R

R

I

I

T

T

A

A

V

V

I

I

P

P

Y

Y

F

F

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
|
R

K

R

T

V

L
x
R

P

S

-

A

R

R

T

V

P

P

I

I

S

T

A

A

K

K

L

V

V

V

A

A

N

D

L

F

I

L

T

S

H

S

A

V

G

G

A

V

D

D

R

R

V

V

L

L

T

T

V

V

D

D

L

L

H
|
H

A

A

G
x
E

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

V

P

P

T

V

D

D

N

N

L

V

Y
x
F

A

G

A
x
S

P

P

V

I

M

L

V

L

R

E

D

D

I

M

T

L

K

Q

R

L

Y

N

T

L

D

D

G

-

N

N

I

P

T

I

V

V

S

S

P

P

D
|
D

V

I

G

G

V

V

V
|
V

R

R

A

A

R

R

S

A

L

I

A

A

S

K

R

L

I

L

-

N

D

D

A

T

P

D

L

M

A

A

I

I

D

D

K

K

R

R

D

-

R

-

P

-

G

-

E

-

S

-

E

Q

V

V

M

M

N

H

V

I

I

I

G

G

N

D

V

V

T

A

G

G

R

R

H

D

C

C

I

V

L

L

I

V

D
|
D

D

D

I
x
M

I

I

D
|
D

S
x
T

G

G

T

T

L

L

C

C

N

K

A

A

H

E

A

A

L

L

I

K

E

E

K

R

G

G

A

A

C

K

S

R

V

V

A

F

A

A

Y

Y

A

A

T

T

H

H

G

P

V

I

F

F

S

S

G

G

G

N

A

A

V

A

A

N

R

N

I

L

Q

R

A

N

S

S

K

V

I

I

D

D

T

E

L

V

V

V

I

V

T

C

D

D

T

T

I

I

V

P

P

L

T

S

E

D

A

E

V

I

R

K

V

S

A

L

S

P

N

N

I

V

E

R

V

T

L

L

S

T

L

L

S

S

A

G

L

M

I

L

G

A

E

E

A

A

I

I

L

R

R

R

T

I

S

S

G

N

E

E

S

S

V

I

S

S

S

A

L

M

F

F

D

E

binding adenosine-5'-diphosphate: F33 (= F32), D35 (= D34), E37 (= E36), R94 (= R93), Q95 (= Q94), R97 (= R96), R97 (= R96), R100 (≠ L99), H129 (= H127), E131 (≠ G129), F145 (≠ Y143), S147 (≠ A145), V173 (= V172)
binding 5-O-phosphono-alpha-D-ribofuranose: D168 (= D167), D212 (= D216), M214 (≠ I218), D216 (= D220), T217 (≠ S221)

Q63XL8 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Burkholderia pseudomallei (strain K96243) (see paper)
55% identity, 100% coverage: 1:309/310 of query aligns to 7:315/318 of Q63XL8

query
sites
Q63XL8

M

L

K

M

L

V

V

F

A

T

G

G

N

N

S

A

N

N

R

P

P

A

L

L

A

A

N

Q

A

E

I

V

A

V

A

K

Y

I

L

L

K

G

T

I

P

P

L

L

T

G

E

K

C

A

L

M

V

V

R

S

R

R

F

F

S

S

D

D

M

G

E

E

I

I

F

Q

V

V

E

E

V

I

R

Q

E

E

N

N

V

V

R

R

G

G

E

K

D

D

I

V

F

F

V

V

I

L

Q

Q

S

S

T

T

S

C

Y

A

P

P

A

T

N

N

D

D

N

N

L

L

M

M

E

E

L

L

L

M

I

I

L

M

T

V

D

D

A

A

L

L

R

K

R

R

A

A

S

S

A

A

R

G

R

R

I

I

T

T

A

A

V

A

I

I

P

P

Y

Y

F

F

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R

R

K

R

T

P

L

R

P

S

-

A

R

R

T

V

P

A

I

I

S

S

A

A

K

K

L

V

V

V

A

A

N

N

L

M

I

L

T

E

H

I

A

A

G

G

A

V

D

E

R

R

V

I

L

I

T

T

V

M

D

D

L

L

H

H

A

A

G

D

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

T

V

D

D

N

N

L

I

Y

Y

A

A

A

T

P

P

V

I

M

L

V

L

R

G

D

D

I

L

T

R

K

K

R

Q

-

N

Y

Y

T

P

D

D

G

-

N

-

I

L

T

L

V

V

S

S

P

P

D

D

V

V

G

G

V

V

R

R

A

A

R

R

S

A

L

L

A

A

S

K

R

Q

I

L

D

N

A

C

P

D

L

L

A

A

I

I

D

D

K

K

R

R

D

P

R

K

P

A

G

N

E

V

S

A

E

E

V

V

M

M

N

N

V

I

I

I

G

G

N

E

V

V

T

E

G

G

R

R

H

T

C

C

I

V

L

I

I

M

D

D

I

M

I

V

D

D

S

T

G

A

G

G

T

T

L

L

C

C

N

K

A

A

H

Q

A

V

L

L

I

K

E

E

K

R

G

G

A

A

C

K

S

Q

V

V

A

F

A

A

Y

Y

A

A

T

T

H

H

G

P

V

V

F

L

S

S

G

G

A

A

V

A

A

D

R

R

I

I

Q

A

A

A

S

S

K

A

I

L

D

D

T

E

L

L

V

V

I

V

T

T

D

D

T

T

I

I

V

P

P

L

T

S

E

A

A

E

V

S

R

L

V

A

A

C

S

P

N

K

I

I

E

R

V

A

L

L

S

S

L

S

S

A

A

G

L

L

I

L

G

A

E

E

A

T

I

F

L

S

R

R

T

I

S

R

G

R

E

G

E

D

S

S

V

V

S

M

S

S

L

L

F

F

RQ 99:100 (= RQ 93:94) binding ATP

3dahC 2.3 a crystal structure of ribose-phosphate pyrophosphokinase from burkholderia pseudomallei (see paper)
55% identity, 98% coverage: 1:305/310 of query aligns to 2:299/300 of 3dahC

query
sites
3dahC

M

L

K

M

L

V

V

F

A

T

G

G

N

N

S

A

N

N

R

P

P

A

L

L

A

A

N

Q

A

E

I

V

A

V

A

K

Y

I

L

L

K

G

T

I

P

P

L

L

T

G

E

K

C

A

L

M

V

V

R

S

R

R

F
|
F

S

S

D
|
D

M

G

E
|
E

I

I

F

Q

V

V

E

E

V

I

R

Q

E

E

N

N

V

V

R

R

G

G

E

K

D

D

I

V

F

F

V

V

I

L

Q

Q

S

S

T

T

S

C

Y

A

P

P

A

T

N

N

D

D

N

N

L

L

M

M

E

E

L

L

L

M

I

I

L

M

T

V

D

D

A

A

L

L

R

K

R

R

A

A

S

S

A

A

R

G

R

R

I

I

T

T

A

A

V

A

I

I

P

P

Y

Y

F

F

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R

R

K

R

T

P

L

R

P

S

-

A

R

R

T

V

P

A

I

I

S

S

A

A

K

K

L

V

V

V

A

A

N

N

L

M

I

L

T

E

H

I

A

A

G

G

A

V

D

E

R

R

V

I

L

I

T

T

V

M

D

D

L

L

H

H

A

A

G

D

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

T

V

D

D

N

N

L

I

Y

Y

A

A

A

T

P

P

V

I

M

L

V

L

R

G

D

D

I

L

T

R

K

K

R

Q

-

N

Y

Y

T

P

D

D

G

-

N

-

I

L

T

L

V

V

S

S

P

P

D

D

V

V

G

G

V

V

R

R

A

A

R

R

S

A

L

L

A

A

S

K

R

Q

I

L

D

N

A

C

P

D

L

L

A

A

I

I

D

D

K

K

R

R

D

-

R

-

P

-

G

-

E

-

S

-

E

-

V

V

M

M

N

N

V

I

I

I

G

G

N

E

V

V

T

E

G

G

R

R

H

T

C

C

I

V

L

I

I

M

D

D

I

M

I

V

D

D

S

T

G

A

G

G

T

T

L

L

C

C

N

K

A

A

H

Q

A

V

L

L

I

K

E

E

K

R

G

G

A

A

C

K

S

Q

V

V

A

F

A

A

Y

Y

A

A

T

T

H

H

G

P

V

V

F

L

S

S

G

G

A

A

V

A

A

D

R

R

I

I

Q

A

A

A

S

S

K

A

I

L

D

D

T

E

L

L

V

V

I

V

T

T

D

D

T

T

I

I

V

P

P

L

T

S

E

A

A

E

V

S

R

L

V

A

A

C

S

P

N

K

I

I

E

R

V

A

L

L

S

S

L

S

S

A

A

G

L

L

I

L

G

A

E

E

A

T

I

F

L

S

R

R

T

I

S

R

G

R

E

G

E

D

S

S

V

V

binding adenosine monophosphate: F33 (= F32), D35 (= D34), E37 (= E36), R94 (= R93), Q95 (= Q94)

6nfeB Crystal structure of ribose-phosphate pyrophosphokinase from legionella pneumophila with bound amp, adp, and ribose-5-phosphate
55% identity, 97% coverage: 1:300/310 of query aligns to 3:298/299 of 6nfeB

query
sites
6nfeB

M

M

K

M

L

I

V

F

A

T

G

G

N

N

S

A

N

N

R

P

P

E

L

L

A

A

N

L

A

K

I

I

A

S

Y

H

L

L

K

Q

T

I

P

P

L

I

T

G

E

K

C

A

L

T

V

V

R

G

R

T

F
|
F

S

S

D
|
D

M

G

E
|
E

I

T

F

M

V

V

E

E

V

I

R

L

E

E

N

N

V

V

R

R

G

G

E

K

D

D

I

V

F

F

V

V

I

L

Q

Q

S

S

T

T

S

C

Y

A

P

P

A

A

N

N

D

N

N

N

L

L

M

M

E

E

L

L

I

I

L

M

T

A

D

D

A

A

L

L

R

R

A

S

S

S

A

A

R

G

R

R

I

I

T

T

A

A

V

V

I

V

P

P

Y

Y

F

F

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R

R

K

R

T

V

L

R

P

S

-

A

R

R

T

V

P

P

I

I

S

T

A

A

K

K

L

V

V

V

A

A

N

D

L

M

I

M

T

A

H

S

A

V

G

G

A

I

D

C

R

R

V

V

L

L

T

T

V

V

D

D

L

L

H
|
H

A

A

G

D

Q

Q

I

I

Q

Q

G

G

F

F

D

Y

I

M

P

P

T

V

D

D

N

N

L

V

Y

Y

A

S

A

T

P

P

V

V

M

L

V

L

R

E

D

D

I

I

T

T

K

K

R

Q

Y

K

T

L

D

N

G

-

N

N

I

I

T

M

V

I

V

V

S

S

P

P

D

D

V

V

G

G

V

V

R

R

A

A

R

R

S

A

L

V

A

A

S

K

R

R

I

L

-

N

D

D

A

A

P

E

L

L

A

S

I

I

D

D

K

K

R

R

D

-

R

-

P

-

G

-

E

-

S

S

E

E

V

V

M

M

N

H

V

I

I

I

G

G

N

E

V

P

T

A

G

N

R

K

H

N

C

C

I

I

L

I

I

V

D
|
D

I
|
I

I

V

D
|
D

S
x
T

G
x
A

G

G

T
|
T

L

L

C

C

N

T

A

A

H

H

A

E

L

L

I

K

E

K

K

N

G

G

A

A

C

K

S

S

V

V

A

R

A

A

Y

Y

A

I

T

T

H

H

G

P

V

V

F

L

S

S

G

G

G

P

A

A

V

V

A

N

R

N

I

I

Q

K

A

H

S

S

K

G

I

L

D

D

T

E

L

V

V

V

I

V

T

T

D

D

T

T

I

I

V

P

P

L

T

S

E

A

A

E

V

A

R

Q

V

N

A

C

S

E

N

K

I

I

E

R

V

V

L

V

S

S

L

L

S

A

A

D

L

M

I

L

G

A

E

Q

A

A

I

I

L

K

R

R

T

V

S

N

binding adenosine-5'-diphosphate: F34 (= F32), D36 (= D34), E38 (= E36), R95 (= R93), Q96 (= Q94), H130 (= H127)
binding 5-O-phosphono-alpha-D-ribofuranose: H130 (= H127), D214 (= D216), D215 (= D217), I216 (= I218), D218 (= D220), T219 (≠ S221), A220 (≠ G222), T222 (= T224)

6nfeA Crystal structure of ribose-phosphate pyrophosphokinase from legionella pneumophila with bound amp, adp, and ribose-5-phosphate
54% identity, 97% coverage: 1:300/310 of query aligns to 3:297/298 of 6nfeA

query
sites
6nfeA

M

M

K

M

L

I

V

F

A

T

G

G

N

N

S

A

N

N

R

P

P

E

L

L

A

A

N

L

A

K

I

I

A

S

Y

H

L

L

K

Q

T

I

P

P

L

I

T

G

E

K

C

A

L

T

V

V

R

G

R

T

F
|
F

S

S

D
|
D

M

G

E
|
E

I

T

F

M

V

V

E

E

V

I

R

L

E

E

N

N

V

V

R

R

G

G

E

K

D

D

I

V

F

F

V

V

I

L

Q

Q

S

S

T

T

S

C

Y

A

P

P

A

A

N

N

D

N

N

N

L

L

M

M

E

E

L

L

I

I

L

M

T

A

D

D

A

A

L

L

R

R

A

S

S

S

A

A

R

G

R

R

I

I

T

T

A

A

V

V

I

V

P

P

Y

Y

F

F

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
|
R

K

R

T
x
V

L

R

P

S

-

A

R

R

T

V

P

P

I

I

S

T

A

A

K

K

L

V

V

V

A

A

N

D

L

M

I

M

T

A

H

S

A

V

G

G

A

I

D

C

R

R

V

V

L

L

T

T

V

V

D

D

L

L

H
|
H

A

A

G

D

Q

Q

I

I

Q

Q

G

G

F

F

D

Y

I

M

P

P

T

V

D

D

N

N

L

V

Y
|
Y

A

S

A

T

P

P

V

V

M

L

V

L

R

E

D

D

I

I

T

T

K

K

R

Q

Y

K

T

L

D

N

G

-

N

N

I

I

T

M

V

I

V

V

S

S

P

P

D

D

V

V

G

G

V

V

R
|
R

A

A

R

R

S
x
A

L

V

A

A

S
x
K

R

R

I

L

-

N

D

D

A

A

P

E

L

L

A

S

I

I

D

D

K

K

R

R

D

-

R

-

P

-

G

-

E

-

S

-

E

E

V

V

M

M

N

H

V

I

I

I

G

G

N

E

V

P

T

A

G

N

R

K

H

N

C

C

I

I

L

I

I

V

D
|
D

I
|
I

I

V

D
|
D

S
x
T

G
x
A

G

G

T
|
T

L

L

C

C

N

T

A

A

H

H

A

E

L

L

I

K

E

K

K

N

G

G

A

A

C

K

S

S

V

V

A

R

A

A

Y

Y

A

I

T

T

H

H

G

P

V

V

F

L

S

S

G

G

G

P

A

A

V

V

A

N

R

N

I

I

Q

K

A

H

S

S

K

G

I

L

D

D

T

E

L

V

V

V

I

V

T

T

D

D

T

T

I

I

V

P

P

L

T

S

E

A

A

E

V

A

R

Q

V

N

A

C

S

E

N

K

I

I

E

R

V

V

L

V

S

S

L

L

S

A

A

D

L

M

I

L

G

A

E

Q

A

A

I

I

L

K

R

R

T

V

S

N

binding adenosine-5'-diphosphate: F34 (= F32), D36 (= D34), E38 (= E36), R95 (= R93), Q96 (= Q94), H130 (= H127)
binding adenosine monophosphate: R98 (= R96), V100 (≠ T98), Y146 (= Y143), R175 (= R173), A178 (≠ S176), K181 (≠ S179)
binding 5-O-phosphono-alpha-D-ribofuranose: H130 (= H127), D213 (= D216), D214 (= D217), I215 (= I218), D217 (= D220), T218 (≠ S221), A219 (≠ G222), T221 (= T224)

P14193 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PPRibP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Bacillus subtilis (strain 168) (see 4 papers)
52% identity, 100% coverage: 1:309/310 of query aligns to 10:316/317 of P14193

query
sites
P14193

M

L

K

K

L

I

V

F

A

S

G

L

N

N

S

S

N

N

R

P

P

E

L

L

A

A

N

K

A

E

I

I

A

A

A

D

Y

I

L

V

K

G

T

V

P

Q

L

L

T

G

E

K

C

C

L

S

V

V

R

T

R

R

F

F

S

S

D

D

M

G

E

E

I

V

F

Q

V

I

E

N

V

I

R

E

E

E

N

S

V

I

R

R

G

G

E

C

D

D

I

C

F

Y

V

I

I

I

Q

Q

S

S

T

T

S

S

Y

D

P

P

A

V

N

N

D

E

N

H

L

I

M

M

E

E

L

L

I

I

L

M

T

V

D

D

A

A

L

L

R

K

R

R

A

A

S

S

A

A

R

K

R

T

I

I

T

N

A

I

V

V

I

I

P

P

Y

Y

F

Y

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R

R

K

K

T

A

L

R

P

S

R

R

T

E

P

P

I

I

S

T

A

A

K

K

L

L

V

F

A

A

N

N

L

L

I

L

T

E

H

T

A

A

G

G

A

A

D

T

R

R

V

V

L

I

T

A

V

L

D

D

L

L

H

H

A

A

G

P

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

T

I

D

D

N

H

L

L

Y

M

A

G

A

V

P

P

V

I

M

L

V

-

R

-

D

-

I

-

T

-

K

G

R

E

Y

Y

T

F

D

E

G

G

-

K

-

N

-

L

-

E

N

D

I

I

T

V

V

I

V

V

S

S

P

P

D

D

V

H

G

G

V

V

V

T

R

R

A

A

R

R

S

K

L

L

A

A

S

D

R

R

I

L

D

K

A

A

P

P

L

I

A

A

I

I

D

D

K
|
K

R

R

R
|
R

D

P

R
|
R

P

P

G
x
N

E

V

S

A

E
|
E

V

V

M

M

N

N

V

I

I

V

G

G

N

N

V

I

T

E

G

G

R

K

H

T

C

A

I

I

L

L

I

I

D

D

I

I

D
|
D

S
x
T

G
x
A

G
|
G

T
|
T

L

I

C

T

N

L

A

A

H

N

A

A

L

L

I

V

E

E

K

N

G

G

A

A

C

K

S

E

V

V

A

Y

A

A

Y

C

A

C

T

T

H

H

G

P

V

V

F

L

S

S

G

G

G

P

A

A

V

V

A

E

R

R

I

I

Q

N

A

N

S

S

K

T

I

I

D

K

T

E

L

L

V

V

I

V

T

T

D

N

T

S

I

I

V

K

P

L

T

P

E

E

A

E

V

K

R

K

V

I

A

-

S

E

N

R

I

F

E

K

V

Q

L

L

S

S

L

V

S

G

A

P

L

L

I

L

G

A

E

E

A

A

I

I

L

I

R

R

T

V

S

H

G

E

E

Q

S

S

V

V

S

S

S

Y

L

L

F

F

RQ 102:103 (= RQ 93:94) binding ATP
K198 (= K190) mutation to A: Strong decrease of the Vmax value compared to that of the wild-type. The affinity binding for ATP and Rib-5-P are slightly altered compared to the wild-type. The cooperativity of ADP binding is reduced.
R200 (= R192) mutation to A: Strong decrease of the Vmax value compared to that of the wild-type enzyme. The affinity binding for ATP and Rib-5-P are slightly altered compared to the wild-type.
R202 (= R194) mutation to A: 3-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
N204 (≠ G196) mutation to A: 4.5-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
E207 (= E199) mutation to A: 2.5-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
DTAGT 228:232 (≠ DSGGT 220:224) binding D-ribose 5-phosphate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

8dbkB Human prps1 with phosphate, atp, and r5p; hexamer with resolved catalytic loops (see paper)
47% identity, 100% coverage: 1:309/310 of query aligns to 3:312/316 of 8dbkB

query
sites
8dbkB

M

I

K

K

L

I

V

F

A

S

G

G

N

S

S

S

N

H

R

Q

P

D

L

L

A

S

N

Q

A

K

I

I

A

A

A

D

Y

R

L

L

K

G

T

L

P

E

L

L

T

G

E

K

C

V

L

V

V

T

R

K

F
|
F

S

S

D
x
N

M

Q

E
|
E

I

T

F

C

V

V

E

E

V

I

R

G

E

E

N
x
S

V

V

R
|
R

G

G

E

E

D

D

I

V

F

Y

V

I

I

V

Q

Q

S

S

T

G

S

C

Y

G

P

E

A

I

N

N

D

D

N

N

L

L

M

M

E

E

L

L

I

I

L

M

T

I

D

N

A

A

L

C

R

K

R

I

A

A

S

S

A

A

R

S

R

R

I

V

T

T

A

A

V

V

I

I

P

P

Y

C

F

F

G

P

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R

K

K

K

T

D

L

K

P

S

R

R

T

A

P

P

I

I

S

S

A

A

K

K

L

L

V

V

A

A

N

N

L

M

I

L

T

S

H

V

A

A

G

G

A

A

D

D

R

H

V

I

L

I

T

T

V

M

D

D

L

L

H
|
H

A

A

G

S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

T

V

D

D

N

N

L

L

Y

Y

A

A

A

E

P

P

V

A

M

V

V

L

R

K

D

W

I

I

T

R

K

E

R

N

Y

I

T

S

D

E

G

W

-

R

N

N

I

C

T

T

V

I

V

V

S

S

P

P

D
|
D

V

A

G
|
G

G

G

V

A

V

K

R

R

A

V

R

T

S

S

L

I

A

A

S

D

R

R

I

L

D

N

A

V

P

D

L

F

A

A

I

L

I

I

D

H

K
|
K

R

E

R
|
R

D

K

R

K

P

A

G
x
N

E

E

S

V

E

D

V

R

M

M

N

V

V

L

I

V

G

G

N

D

V

V

T

K

G

D

R

R

H

V

C

A

I

I

L

L

I

V

D
|
D

I

M

I

A

D
|
D

S
x
T

G
x
C

G
|
G

T
|
T

L

I

C

C

N

H

A

A

H

D

A

K

L

L

I

L

E

S

K

A

G

G

A

A

C

T

S

R

V

V

A

Y

A

A

Y

I

A

L

T

T

H

H

G

G

V

I

F

F

S

S

G

G

G

P

A

A

V

I

A

S

R

R

I

I

Q

N

A

N

S

A

K

C

I

F

D

E

T

A

L

V

V

V

I

V

T

T

D

N

T

T

I

I

V

P

P

Q

T

E

E

D

A

K

V

M

R

K

V

H

A

C

S

S

N

K

I

I

E

Q

V

V

L

I

S

D

L

I

S

S

A

M

L

I

I

L

G

A

E

E

A

A

I

I

L

R

R

R

T

T

S

H

G

N

E

G

E

E

S

S

V

V

S

S

S

Y

L

L

F

F

binding adenosine monophosphate: R95 (= R93), Q96 (= Q94), N199 (≠ G196)
binding adenosine-5'-triphosphate: F34 (= F32), N36 (≠ D34), E38 (= E36)
binding phosphate ion: S46 (≠ N44), R48 (= R46)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: H129 (= H127), D170 (= D167), G172 (= G169), K193 (= K190), R195 (= R192), D219 (= D216), D220 (= D217), D223 (= D220), T224 (≠ S221), C225 (≠ G222), G226 (= G223), T227 (= T224)

8dbeA Human prps1 with adp; hexamer (see paper)
47% identity, 100% coverage: 1:309/310 of query aligns to 3:312/316 of 8dbeA

query
sites
8dbeA

M

I

K

K

L

I

V

F

A

S

G

G

N

S

S

S

N

H

R

Q

P

D

L

L

A

S

N

Q

A

K

I

I

A

A

A

D

Y

R

L

L

K

G

T

L

P

E

L

L

T

G

E

K

C

V

L

V

V

T

R

K

F
|
F

S

S

D
x
N

M

Q

E
|
E

I

T

F

C

V

V

E

E

V

I

R

G

E

E

N

S

V

V

R

R

G

G

E

E

D

D

I

V

F

Y

V

I

I

V

Q

Q

S

S

T

G

S

C

Y

G

P

E

A

I

N

N

D

D

N

N

L

L

M

M

E

E

L

L

I

I

L

M

T

I

D

N

A

A

L

C

R

K

R

I

A

A

S

S

A

A

R

S

R

R

I

V

T

T

A

A

V

V

I

I

P

P

Y

C

F

F

G

P

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
x
K

K
|
K

T
x
D

L

K

P
x
S

R
|
R

T

A

P

P

I

I

S

S

A

A

K

K

L

L

V

V

A

A

N

N

L

M

I

L

T

S

H

V

A

A

G

G

A

A

D

D

R

H

V

I

L

I

T

T

V

M

D

D

L

L

H
|
H

A

A

G

S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

T

V

D
|
D

N

N

L

L

Y
|
Y

A

A

A

E

P

P

V

A

M

V

V

L

R

K

D

W

I

I

T

R

K

E

R

N

Y

I

T

S

D

E

G

W

-

R

N

N

I

C

T

T

V

I

V

V

S

S

P

P

D
|
D

V

A

G

G

V

A

V

K

R

R

A

V

R

T

S

S

L

I

A

A

S

D

R

R

I

L

D

N

A

V

P

D

L

F

A

A

I

L

I

I

D

H

K

K

R

E

R

R

D

K

R

K

P

A

G

N

E

E

S

V

E

D

V

R

M

M

N

V

V

L

I

V

G

G

N

D

V

V

T

K

G

D

R

R

H

V

C

A

I

I

L

L

I

V

D
|
D

I

M

I

A

D
|
D

S
x
T

G

C

G
|
G

T
|
T

L

I

C

C

N

H

A

A

H

D

A

K

L

L

I

L

E

S

K

A

G

G

A

A

C

T

S

R

V

V

A

Y

A

A

Y

I

A

L

T

T

H

H

G

G

V

I

F

F

S

S

G

G

G

P

A

A

V

I

A

S

R

R

I

I

Q

N

A

N

S

A

K

C

I

F

D

E

T

A

L

V

V

V

I

V

T

T

D

N

T

T

I

I

V

P

P

Q

T

E

E

D

A

K

V

M

R

K

V

H

A

C

S

S

N

K

I

I

E

Q

V

V

L

I

S

D

L

I

S

S

A

M

L

I

I

L

G

A

E

E

A

A

I

I

L

R

R

R

T

T

S

H

G

N

E

G

E

E

S
|
S

V
|
V

S
|
S

S

Y

L

L

F
|
F

binding adenosine-5'-diphosphate: F34 (= F32), N36 (≠ D34), E38 (= E36), R95 (= R93), Q96 (= Q94), K98 (≠ R96), K99 (= K97), D100 (≠ T98), S102 (≠ P100), R103 (= R101), H129 (= H127), D142 (= D140), Y145 (= Y143), S307 (= S304), V308 (= V305), S309 (= S306), F312 (= F309)
binding 5-O-phosphono-alpha-D-ribofuranose: H129 (= H127), D170 (= D167), D219 (= D216), D220 (= D217), D223 (= D220), T224 (≠ S221), G226 (= G223), T227 (= T224)

P60891 Ribose-phosphate pyrophosphokinase 1; PPRibP; Phosphoribosyl pyrophosphate synthase I; PRS-I; EC 2.7.6.1 from Homo sapiens (Human) (see 5 papers)
47% identity, 100% coverage: 1:309/310 of query aligns to 4:313/318 of P60891

query
sites
P60891

M

I

K

K

L

I

V

F

A

S

G

G

N

S

S

S

N

H

R

Q

P

D

L

L

A
x
S

N

Q

A

K

I

I

A

A

A

D

Y

R

L

L

K

G

T

L

P

E

L

L

T

G

E

K

C

V

L

V

V

T

R

K

F

F

S

S

D

N

M

Q

E

E

I

T

F

C

V

V

E

E

V

I

R

G

E

E

N

S

V

V

R

R

G

G

E

E

D
|
D

I

V

F

Y

V

I

I

V

Q

Q

S

S

T

G

S

C

Y

G

P

E

A

I

N

N

D

D

N

N

L

L

M

M

E

E

L

L

I

I

L

M

T

I

D

N

A

A

L

C

R

K

R

I

A

A

S

S

A

A

R

S

R

R

I

V

T

T

A

A

V

V

I

I

P

P

Y

C

F

F

G

P

Y

Y

A

A

R

R

Q

Q

D

D

R

K

K

K

T

D

L

K

P

S

R

R

T

A

P

P

I

I

S

S

A

A

K

K

L

L

V

V

A

A

N
|
N

L

M

I

L

T

S

H

V

A

A

G

G

A

A

D

D

R

H

V

I

L

I

T

T

V

M

D

D

L
|
L

H

H

A

A

G
x
S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

T
x
V

D

D

N
|
N

L

L

Y
|
Y

A

A

A

E

P

P

V

A

M

V

V

L

R

K

D

W

I

I

T

R

K

E

R

N

Y

I

T

S

D

E

G

W

-

R

N

N

I

C

T

T

V

I

V

V

S

S

P

P

D

D

V

A

G

G

V

A

V

K

R

R

A

V

R

T

S

S

L

I

A

A

S
x
D

R

R

I

L

D

N

A

V

P

D

L

F

A
|
A

I

L

I

I

D
x
H

K

K

R

E

R

R

D

K

R

K

P

A

G

N

E

E

S

V

E
x
D

V

R

M

M

N

V

V

L

I

V

G

G

N

D

V

V

T

K

G

D

R

R

H

V

C

A

I

I

L

L

I
x
V

D

D

I

M

I

A

D

D

S

T

G

C

G

G

T

T

L

I

C

C

N
x
H

A

A

H

D

A

K

L

L

I

L

E

S

K

A

G

G

A

A

C

T

S

R

V

V

A

Y

A

A

Y

I

A

L

T

T

H

H

G

G

V

I

F

F

S

S

G

G

G

P

A

A

V

I

A

S

R

R

I

I

Q

N

A

N

S

A

K

C

I

F

D

E

T

A

L

V

V

V

I

V

T

T

D

N

T

T

I

I

V

P

P

Q

T

E

E

D

A

K

V

M

R

K

V

H

A

C

S

S

N

K

I

I

E

Q

V

V

L

I

S

D

L

I

S

S

A

M

L

I

I

L

G

A

E

E

A

A

I

I

L

R

R

R

T

T

S

H

G

N

E

G

E

E

S

S

V

V

S

S

S

Y

L

L

F

F

S16 (≠ A13) to P: found in patients with phosphoribosyl pyrophosphate synthetase I deficiency; likely pathogenic; dbSNP:rs869025594
D52 (= D49) to H: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852542
N114 (= N111) to S: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852540
L129 (= L126) to I: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852543
S132 (≠ G129) mutation to A: Reduces catalytic activity.; mutation to F: No effect on catalytic activity.
V142 (≠ T139) to L: found in a patient with an intermediate phenotype between ARTS and PRPS1 superactivity; likely pathogenic; normal PRPP synthetase activity in fibroblasts; loss of activity in erythrocytes; dbSNP:rs398122855
N144 (= N141) mutation to H: No effect on catalytic activity.
Y146 (= Y143) mutation to F: No effect on catalytic activity.; mutation to M: Reduces catalytic activity.
D183 (≠ S179) to H: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852541
A190 (= A186) to V: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852544
H193 (≠ D189) to Q: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852545
D203 (≠ E199) to H: in a breast cancer sample; somatic mutation
V219 (≠ I215) to G: in a breast cancer sample; somatic mutation
H231 (≠ N227) to D: in a colorectal cancer sample; somatic mutation

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1ibsB Phosphoribosyldiphosphate synthetase in complex with cadmium ions (see paper)
50% identity, 100% coverage: 1:309/310 of query aligns to 4:299/299 of 1ibsB

query
sites
1ibsB

M

L

K

K

L

I

V

F

A

S

G

L

N

N

S

S

N

N

R

P

P

E

L

L

A

A

N

K

A

E

I

I

A

A

A

D

Y

I

L

V

K

G

T

V

P

Q

L

L

T

G

E

K

C

C

L

S

V

V

R

T

R

R

F

F

S

S

D

D

M

G

E

E

I

V

F

Q

V

I

E

N

V

I

R

E

E

E

N

S

V

I

R

R

G

G

E

C

D

D

I

C

F

Y

V

I

I

I

Q

Q

S

S

T

T

S

S

Y

D

P

P

A

V

N

N

D

E

N

H

L

I

M

M

E

E

L

L

I

I

L

M

T

V

D

D

A

A

L

L

R

K

R

R

A

A

S

S

A

A

R

K

R

T

I

I

T

N

A

I

V

V

I

I

P

P

Y

Y

F

Y

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
|
R

K

K

T

S

L

-

P

-

R

R

T

E

P

P

I

I

S

T

A

A

K

K

L

L

V

F

A

A

N

N

L

L

I

L

T

E

H

T

A

A

G

G

A

A

D

T

R

R

V

V

L

I

T

A

V

L

D

D

L

L

H

H

A

A

G

P

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

T

I

D

D

N

H

L

L

Y

M

A

G

A

V

P

P

V

I

M

L

V

-

R

-

D

-

I

-

T

-

K

G

R

E

Y

Y

T

F

D

E

G

G

-

K

-

N

-

L

-

E

N

D

I

I

T

V

V

I

V

V

S

S

P

P

D

D

V

H

G

G

V

V

V

T

R

R

A

A

R

R

S

K

L

L

A

A

S

D

R

R

I

L

D

K

A

A

P

P

L

I

A

A

I

I

D

D

K

K

R

R

R

-

D

-

R

-

P

-

G

-

E

-

S

-

E

-

V

-

M

M

N

N

V

I

I

V

G

G

N

N

V

I

T

E

G

G

R

K

H

T

C

A

I

I

L

L

I

I

D

D

I

I

D

D

S

T

G

A

G

G

T

T

L

I

C

T

N

L

A

A

H

N

A

A

L

L

I

V

E

E

K

N

G

G

A

A

C

K

S

E

V

V

A

Y

A

A

Y

C

A

C

T

T

H

H

G

P

V

V

F

L

S

S

G

G

G

P

A

A

V

V

A

E

R

R

I

I

Q

N

A

N

S

S

K

T

I

I

D

K

T

E

L

L

V

V

I

V

T

T

D

N

T

S

I

I

V

K

P

L

T

P

E

E

A

E

V

K

R

K

V

I

A

-

S

E

N

R

I

F

E

K

V

Q

L

L

S

S

L

V

S

G

A

P

L

L

I

L

G

A

E

E

A

A

I

I

L

I

R

R

T

V

S

H

G

E

E

Q

S

S

V

V

S

S

S

Y

L

L

F

F

binding methyl phosphonic acid adenosine ester: R96 (= R93), Q97 (= Q94), R99 (= R96)

1ibsA Phosphoribosyldiphosphate synthetase in complex with cadmium ions (see paper)
50% identity, 100% coverage: 1:309/310 of query aligns to 2:297/297 of 1ibsA

query
sites
1ibsA

M

L

K

K

L

I

V

F

A

S

G

L

N

N

S

S

N

N

R

P

P

E

L

L

A

A

N

K

A

E

I

I

A

A

A

D

Y

I

L

V

K

G

T

V

P

Q

L

L

T

G

E

K

C

C

L

S

V

V

R

T

R

R

F

F

S

S

D

D

M

G

E

E

I

V

F

Q

V

I

E

N

V

I

R

E

E

E

N

S

V

I

R

R

G

G

E

C

D

D

I

C

F

Y

V

I

I

I

Q

Q

S

S

T

T

S

S

Y

D

P

P

A

V

N

N

D

E

N

H

L

I

M

M

E

E

L

L

I

I

L

M

T

V

D

D

A

A

L

L

R

K

R

R

A

A

S

S

A

A

R

K

R

T

I

I

T

N

A

I

V

V

I

I

P

P

Y

Y

F

Y

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
|
R

K

K

T

S

L

-

P

-

R

R

T

E

P

P

I

I

S

T

A

A

K

K

L

L

V

F

A

A

N

N

L

L

I

L

T

E

H

T

A

A

G

G

A

A

D

T

R

R

V

V

L

I

T

A

V

L

D

D

L

L

H
|
H

A

A

G

P

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

T

I

D

D

N

H

L

L

Y

M

A

G

A

V

P

P

V

I

M

L

V

-

R

-

D

-

I

-

T

-

K

G

R

E

Y

Y

T

F

D

E

G

G

-

K

-

N

-

L

-

E

N

D

I

I

T

V

V

I

V

V

S

S

P

P

D

D

V

H

G

G

V

V

V

T

R

R

A

A

R

R

S

K

L

L

A

A

S

D

R

R

I

L

D

K

A

A

P

P

L

I

A

A

I

I

D

D

K

K

R

R

R

-

D

-

R

-

P

-

G

-

E

-

S

-

E

-

V

-

M

M

N

N

V

I

I

V

G

G

N

N

V

I

T

E

G

G

R

K

H

T

C

A

I

I

L

L

I

I

D

D

I

I

D
|
D

S
x
T

G
x
A

G

G

T
|
T

L

I

C

T

N

L

A

A

H

N

A

A

L

L

I

V

E

E

K

N

G

G

A

A

C

K

S

E

V

V

A

Y

A

A

Y

C

A

C

T

T

H

H

G

P

V

V

F

L

S

S

G

G

G

P

A

A

V

V

A

E

R

R

I

I

Q

N

A

N

S

S

K

T

I

I

D

K

T

E

L

L

V

V

I

V

T

T

D

N

T

S

I

I

V

K

P

L

T

P

E

E

A

E

V

K

R

K

V

I

A

-

S

E

N

R

I

F

E

K

V

Q

L

L

S

S

L

V

S

G

A

P

L

L

I

L

G

A

E

E

A

A

I

I

L

I

R

R

T

V

S

H

G

E

E

Q

S

S

V

V

S

S

S

Y

L

L

F

F

binding methyl phosphonic acid adenosine ester: R94 (= R93), Q95 (= Q94), R97 (= R96), H126 (= H127)
binding sulfate ion: D209 (= D220), T210 (≠ S221), A211 (≠ G222), T213 (= T224)

1dkuA Crystal structures of bacillus subtilis phosphoribosylpyrophosphate synthetase: molecular basis of allosteric inhibition and activation. (see paper)
50% identity, 100% coverage: 1:309/310 of query aligns to 2:295/295 of 1dkuA

query
sites
1dkuA

M

L

K

K

L

I

V

F

A

S

G

L

N

N

S

S

N

N

R

P

P

E

L

L

A

A

N

K

A

E

I

I

A

A

A

D

Y

I

L

V

K

G

T

V

P

Q

L

L

T

G

E

K

C

C

L

S

V

V

R

T

R

R

F

F

S

S

D

D

M

G

E

E

I

V

F

Q

V

I

E

N

V

I

R

E

E

E

N

S

V

I

R

R

G

G

E

C

D

D

I

C

F

Y

V

I

I

I

Q

Q

S

S

T

T

S

S

Y

D

P

P

A

V

N

N

D

E

N

H

L

I

M

M

E

E

L

L

I

I

L

M

T

V

D

D

A

A

L

L

R

K

R

R

A

A

S

S

A

A

R

K

R

T

I

I

T

N

A

I

V

V

I

I

P

P

Y

Y

F

Y

G

G

Y

Y

A

A

R
|
R

Q

Q

D

D

R

R

K
|
K

T

A

L

R

P
x
S

R
|
R

T

E

P

P

I

I

S

T

A

A

K

K

L

L

V

F

A

A

N

N

L

L

I

L

T

E

H

T

A

A

G

G

A

A

D

T

R

R

V

V

L

I

T

A

V

L

D

D

L

L

H
|
H

A

A

G

P

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

T

I

D
|
D

N

H

L

L

Y

M

A

G

A

V

P

P

V

I

M

L

V

-

R

-

D

-

I

-

T

-

K

G

R

E

Y

Y

T

F

D

E

G

G

-

K

-

N

-

L

-

E

N

D

I

I

T

V

V

I

V

V

S

S

P

P

D

D

V

H

G

G

V

V

V

T

R

R

A

A

R

R

S

K

L

L

A

A

S

D

R

R

I

L

D

K

A

A

P

P

L

I

A

A

I

I

D

D

K

K

R

R

R

-

D

-

R

-

P

-

G

-

E

-

S

-

E

-

V

-

M

M

N

N

V

I

I

V

G

G

N

N

V

I

T

E

G

G

R

K

H

T

C

A

I

I

L

L

I

I

D

D

I

I

D

D

S

T

G

A

G

G

T

T

L

I

C

T

N

L

A

A

H

N

A

A

L

L

I

V

E

E

K

N

G

G

A

A

C

K

S

E

V

V

A

Y

A

A

Y

C

A

C

T

T

H

H

G

P

V

V

F

L

S

S

G

G

G

P

A

A

V

V

A

E

R

R

I

I

Q

N

A

N

S

S

K

T

I

I

D

K

T

E

L

L

V

V

I

V

T

T

D

N

T

S

I

I

V

-

P

-

T

-

E

-

A

K

V

L

R

K

V

I

A

-

S

E

N

R

I

F

E

K

V

Q

L

L

S

S

L

V

S

G

A

P

L

L

I

L

G

A

E

E

A

A

I

I

L

I

R

R

T

V

S

H

G

E

E

Q

S
|
S

V
|
V

S
|
S

S

Y

L

L

F
|
F

binding methyl phosphonic acid adenosine ester: R94 (= R93), H128 (= H127)
binding phosphomethylphosphonic acid adenosyl ester: K98 (= K97), S101 (≠ P100), R102 (= R101), D141 (= D140), S290 (= S304), V291 (= V305), S292 (= S306), F295 (= F309)

2hcrA Crystal structure of human phosphoribosyl pyrophosphate synthetase 1 in complex with amp(atp), cadmium and sulfate ion (see paper)
47% identity, 100% coverage: 1:309/310 of query aligns to 2:305/305 of 2hcrA

query
sites
2hcrA

M

I

K

K

L

I

V

F

A

S

G

G

N

S

S

S

N

H

R

Q

P

D

L

L

A

S

N

Q

A

K

I

I

A

A

A

D

Y

R

L

L

K

G

T

L

P

E

L

L

T

G

E

K

C

V

L

V

V

T

R

K

F

F

S

S

D

N

M

Q

E

E

I

T

F

C

V

V

E

E

V

I

R

G

E

E

N

S

V

V

R

R

G

G

E

E

D

D

I

V

F

Y

V

I

I

V

Q

Q

S

S

T

G

S

C

Y

G

P

E

A

I

N

N

D

D

N

N

L

L

M

M

E

E

L

L

I

I

L

M

T

I

D

N

A

A

L

C

R

K

R

I

A

A

S

S

A

A

R

S

R

R

I

V

T

T

A

A

V

V

I

I

P

P

Y

C

F

F

G

P

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
x
K

K

K

T

D

L

K

P

S

R

R

T

A

P

P

I

I

S

S

A

A

K

K

L

L

V

V

A

A

N

N

L

M

I

L

T

S

H

V

A

A

G

G

A

A

D

D

R

H

V

I

L

I

T

T

V

M

D

D

L

L

H
|
H

A

A

G

S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

T

V

D

D

N

N

L

L

Y

Y

A

A

A

E

P

P

V

A

M

V

V

L

R

K

D

W

I

I

T

R

K

E

R

N

Y

I

T

S

D

E

G

W

-

R

N

N

I

C

T

T

V

I

V

V

S

S

P

P

D

D

V

A

G

G

V

A

V

K

R

R

A

V

R

T

S

S

L

I

A

A

S

D

R

R

I

L

D

N

A

V

P

D

L

F

A

A

I

L

I

I

D

H

K

K

R

E

R

R

D

D

R

R

P

-

G

-

E

-

S

-

E

-

V

-

M

M

N

V

V

L

I

V

G

G

N

D

V

V

T

K

G

D

R

R

H

V

C

A

I

I

L

L

I

V

D

D

I

M

I

A

D

D

S

T

G

C

G

G

T

T

L

I

C

C

N

H

A

A

H

D

A

K

L

L

I

L

E

S

K

A

G

G

A

A

C

T

S

R

V

V

A

Y

A

A

Y

I

A

L

T

T

H

H

G

G

V

I

F

F

S

S

G

G

G

P

A

A

V

I

A

S

R

R

I

I

Q

N

A

N

S

A

K

C

I

F

D

E

T

A

L

V

V

V

I

V

T

T

D

N

T

T

I

I

V

P

P

Q

T

E

E

D

A

K

V

M

R

K

V

H

A

C

S

S

N

K

I

I

E

Q

V

V

L

I

S

D

L

I

S

S

A

M

L

I

I

L

G

A

E

E

A

A

I

I

L

R

R

R

T

T

S

H

G

N

E

G

E

E

S

S

V

V

S

S

S

Y

L

L

F

F

binding adenosine monophosphate: R94 (= R93), Q95 (= Q94), K97 (≠ R96), H128 (= H127)

8dbgA Human prps1 with phosphate and atp; hexamer (see paper)
47% identity, 100% coverage: 1:309/310 of query aligns to 3:305/309 of 8dbgA

query
sites
8dbgA

M

I

K

K

L

I

V

F

A

S

G

G

N

S

S

S

N

H

R

Q

P

D

L

L

A

S

N

Q

A

K

I

I

A

A

A

D

Y

R

L

L

K

G

T

L

P

E

L

L

T

G

E

K

C

V

L

V

V

T

R

K

F
|
F

S

S

D
x
N

M

Q

E
|
E

I

T

F

C

V

V

E

E

V

I

R

G

E

E

N
x
S

V

V

R
|
R

G

G

E

E

D

D

I

V

F

Y

V

I

I

V

Q

Q

S

S

T

G

S

C

Y

G

P

E

A

I

N

N

D

D

N

N

L

L

M

M

E

E

L

L

I

I

L

M

T

I

D

N

A

A

L

C

R

K

R

I

A

A

S

S

A

A

R

S

R

R

I

V

T

T

A

A

V

V

I

I

P

P

Y

C

F

F

G

P

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
x
K

K

K

T

D

L

K

P

S

R

R

T

A

P

P

I

I

S

S

A

A

K

K

L

L

V

V

A

A

N

N

L

M

I

L

T

S

H

V

A

A

G

G

A

A

D

D

R

H

V

I

L

I

T

T

V

M

D

D

L

L

H
|
H

A

A

G

S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

T

V

D

D

N

N

L

L

Y

Y

A

A

A

E

P

P

V

A

M

V

V

L

R

K

D

W

I

I

T

R

K

E

R

N

Y

I

T

S

D

E

G

W

-

R

N

N

I

C

T

T

V

I

V

V

S

S

P

P

D

D

V

A

G

G

V

A

V

K

R

R

A

V

R

T

S

S

L

I

A

A

S

D

R

R

I

L

D

N

A

V

P

D

L

F

A

A

I

L

I

I

D

H

K

K

R

-

R

E

D

D

R

R

P

-

G

-

E

-

S

-

E

-

V

-

M

M

N

V

V

L

I

V

G

G

N

D

V

V

T

K

G

D

R

R

H

V

C

A

I

I

L

L

I

V

D

D

I

M

I

A

D
|
D

S
x
T

G
x
C

G

G

T
|
T

L

I

C

C

N

H

A

A

H

D

A

K

L

L

I

L

E

S

K

A

G

G

A

A

C

T

S

R

V

V

A

Y

A

A

Y

I

A

L

T

T

H

H

G

G

V

I

F

F

S

S

G

G

G

P

A

A

V

I

A

S

R

R

I

I

Q

N

A

N

S

A

K

C

I

F

D

E

T

A

L

V

V

V

I

V

T

T

D

N

T

T

I

I

V

P

P

Q

T

E

E

D

A

K

V

M

R

K

V

H

A

C

S

S

N

K

I

I

E

Q

V

V

L

I

S

D

L

I

S

S

A

M

L

I

I

L

G

A

E

E

A

A

I

I

L

R

R

R

T

T

S

H

G

N

E

G

E

E

S

S

V

V

S

S

S

Y

L

L

F

F

binding adenosine-5'-triphosphate: F34 (= F32), N36 (≠ D34), E38 (= E36), R95 (= R93), Q96 (= Q94), K98 (≠ R96), H129 (= H127)
binding phosphate ion: S46 (≠ N44), R48 (= R46), D216 (= D220), T217 (≠ S221), C218 (≠ G222), T220 (= T224)

7yk1A Structural basis of human prps2 filaments (see paper)
46% identity, 99% coverage: 3:309/310 of query aligns to 5:303/306 of 7yk1A

query
sites
7yk1A

L

L

V

F

A

S

G

G

N

S

S

S

N

H

R

Q

P

D

L

L

A

S

N

Q

A

R

I

V

A

A

A

D

Y

R

L

L

K

G

T

L

P

E

L

L

T

G

E

K

C

V

L

V

V

T

R

K

F
|
F

S

S

D
x
N

M

Q

E
|
E

I

T

F

S

V

V

E

E

V

I

R

G

E

E

N
x
S

V

V

R
|
R

G

G

E

E

D

D

I

V

F

Y

V

I

I

I

Q

Q

S

S

T

G

S

C

Y

G

P

E

A

I

N

N

D

D

N

N

L

L

M

M

E

E

L

L

I

I

L

M

T

I

D

N

A

A

L

C

R

K

R

I

A

A

S

S

A

S

R

S

R

R

I

V

T

T

A

A

V

V

I

I

P

P

Y

C

F

F

G

P

Y

Y

A

A

R
|
R

Q

Q

D

D

R

K

K
|
K

T
x
D

L
x
K

P
x
S

R
|
R

T

A

P

P

I

I

S

S

A

A

K

K

L

L

V

V

A

A

N

N

L

M

I

L

T

S

H

V

A

A

G

G

A

A

D

D

R

H

V

I

L

I

T

T

V

M

D

D

L

L

H
|
H

A

A

G

S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

T

V

D
|
D

N

N

L

L

Y

Y

A

A

A

E

P

P

V

A

M

V

V

L

R

Q

D

W

I

I

T

R

K

E

R

N

Y

I

T

A

D

E

G

W

-

K

N

N

I

C

T

I

V

I

V

V

S

S

P

P

D

D

V

A

G

G

V

A

V

K

R

R

A

V

R

T

S

S

L

I

A

A

S

D

R

R

I

L

D

N

A

V

P

E

L

F

A

A

I

L

I

I

D

H

K

K

R

E

R

-

D

-

R

-

P

-

G

-

E

-

S

-

E

-

V

-

M

M

N

V

V

L

I

V

G

G

N

D

V

V

T

K

G

D

R

R

H

V

C

A

I

I

L

L

I

V

D

D

I

M

I

A

D
|
D

S

T

G
x
C

G

G

T
|
T

L

I

C

C

N

H

A

A

H

D

A

K

L

L

I

L

E

S

K

A

G

G

A

A

C

T

S

K

V

V

A

Y

A

A

Y

I

A

L

T

T

H

H

G

G

V

I

F

F

S

S

G

G

G

P

A

A

V

I

A

S

R

R

I

I

Q

N

A

N

S

A

K

A

I

F

D

E

T

A

L

V

V

V

I

V

T

T

D

N

T

T

I

I

V

P

P

Q

T

E

E

D

A

K

V

M

R

K

V

H

A

C

S

T

N

K

I

I

E

Q

V

V

L

I

S

D

L

I

S

S

A

M

L

I

I

L

G

A

E

E

A

A

I

I

L

R

R

R

T

T

S

H

G

N

E

G

E

E

S
|
S

V

V

S
|
S

S

Y

L

L

F
|
F

binding adenosine-5'-diphosphate: F34 (= F32), N36 (≠ D34), E38 (= E36), S46 (≠ N44), R48 (= R46), R95 (= R93), K99 (= K97), D100 (≠ T98), K101 (≠ L99), S102 (≠ P100), R103 (= R101), H129 (= H127), D142 (= D140), S298 (= S304), S300 (= S306), F303 (= F309)
binding phosphate ion: D214 (= D220), C216 (≠ G222), T218 (= T224)

7pn0A Crystal structure of the phosphorybosylpyrophosphate synthetase ii from thermus thermophilus at r32 space group
46% identity, 99% coverage: 3:309/310 of query aligns to 5:307/312 of 7pn0A

query
sites
7pn0A

L

I

V

F

A

S

G

G

N

Q

S

S

N

N

R

R

P

P

L

L

A

A

N

Q

A

A

I

I

A

A

A

E

Y

A

L

L

K

G

T

L

P

P

L

L

T

G

E

K

C

S

L

T

V

T

R

L

R

R

F

F

S

A

D

N

M

D

E

N

I

L

F

F

V

V

E

R

V

Y

R

E

E

E

N

S

V

L

R

R

G

E

E

G

D

D

I

V

F

F

V

I

I

V

Q

Q

S

S

T

F

S

V

Y

P

P

P

A

V

N

Q

D

D

N

H

L

L

M

M

E

E

L

L

I

M

L

M

T

V

D

D

A

A

L

A

R

K

R

G

A

A

S

S

A

A

R

A

R

R

I

V

T

T

A

A

V

V

I

I

P

P

Y

Y

F

F

G

S

Y

Y

A

A

R
|
R

Q
x
S

D

D

R

K

K

K

T

D

L

A

P

P

R

R

T

I

P

S

I

I

S

T

A

A

K

R

L

L

V

I

A

A

N

D

L

L

I

L

T

Q

H

T

A

A

G

G

A

A

D

D

R

R

V

V

L

L

T

T

V

M

D

T

L

L

H
|
H

A

S

G

P

Q

Q

I

V

Q

H

G

G

F

F

D

K

I

I

P

P

T

V

D

D

N

H

L

L

Y

S

A

A

A

E

P

P

V

V

M

I

V

A

R

N

D

Y

I

F

T

A

K

T

R

R

Y

V

T

D

D

L

G

E

N

N

I

A

T

V

V

V

S

A

P

P

D

D

V

A

G

G

G

D

V

L

V

K

R

R

A

A

R

S

S

A

L

L

A

A

S

R

R

R

I

L

D

G

A

L

P

P

L

L

A

A

I

F

I

I

D

D

K

K

R

E

R

R

D

V

R

S

P

D

G

T

E

E

S

V

E

R

V

V

M

R

N

M

V

L

I

V

G

G

N

E

V

V

T

E

G

G

R

K

H

T

C

A

I

L

L

I

I

V

D

D

I

E

I

I

D

S

S

T

G

A

G

G

T

S

L

L

C

V

N

E

A

A

A

V

H

E

A

A

L

L

I

M

E

Q

K

A

G

G

A

A

C

K

S

E

V

V

A

Y

A

A

Y

A

A

A

T

T

H

H

G

G

V

V

F

Y

S

V

G

G

G

P

A

A

V

L

A

D

R

R

I

I

Q

A

A

K

S

S

K

P

I

V

D

K

T

E

L

V

V

A

I

A

T

T

D

D

T

T

I

C

V

P

P

P

T

K

E

E

A

G

V

P

R

K

V

-

A

-

S

-

N

-

I

L

E

R

V

T

L

L

S

T

L

V

S

A

A

P

L

L

I

F

G

A

E

E

A

A

I

I

L

W

R

R

T

I

S

H

G

R

E

G

E

E

S

S

V

V

S

S

L

L

F

F

binding adenosine-5'-diphosphate: R95 (= R93), S96 (≠ Q94), H129 (= H127)

Query Sequence

>WP_013419358.1 NCBI__GCF_000166055.1:WP_013419358.1
MKLVAGNSNRPLANAIAAYLKTPLTECLVRRFSDMEIFVEVRENVRGEDIFVIQSTSYPA
NDNLMELLILTDALRRASARRITAVIPYFGYARQDRKTLPRTPISAKLVANLITHAGADR
VLTVDLHAGQIQGFFDIPTDNLYAAPVMVRDITKRYTDGNITVVSPDVGGVVRARSLASR
IDAPLAIIDKRRDRPGESEVMNVIGNVTGRHCILIDDIIDSGGTLCNAAHALIEKGACSV
AAYATHGVFSGGAVARIQASKIDTLVITDTIVPTEAVRVASNIEVLSLSALIGEAILRTS
GEESVSSLFD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory