SitesBLAST
Comparing WP_013419585.1 NCBI__GCF_000166055.1:WP_013419585.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
5z20F The ternary structure of d-lactate dehydrogenase from pseudomonas aeruginosa with nadh and oxamate (see paper)
58% identity, 94% coverage: 1:327/347 of query aligns to 8:333/336 of 5z20F
- active site: S108 (≠ A101), R241 (= R234), D265 (= D259), E270 (= E264), H302 (= H296)
- binding 1,4-dihydronicotinamide adenine dinucleotide: Y107 (= Y100), G160 (= G153), Q161 (≠ K154), I162 (= I155), Y180 (= Y173), D181 (= D174), P182 (≠ I175), C212 (= C205), P213 (= P206), T218 (= T211), T239 (= T232), G240 (≠ A233), R241 (= R234), H302 (= H296), A304 (= A298)
4cukA Structure of salmonella d-lactate dehydrogenase in complex with nadh
52% identity, 94% coverage: 1:326/347 of query aligns to 1:325/330 of 4cukA
- active site: S101 (≠ A101), R234 (= R234), D258 (= D259), E263 (= E264), H295 (= H296)
- binding 1,4-dihydronicotinamide adenine dinucleotide: Y100 (= Y100), G153 (= G153), K154 (= K154), I155 (= I155), F173 (≠ Y173), D174 (= D174), P175 (≠ I175), H204 (= H204), C205 (= C205), P206 (= P206), N211 (≠ T211), T232 (= T232), Y260 (= Y261), H295 (= H296), A297 (= A298)
4zgsA Identification of the pyruvate reductase of chlamydomonas reinhardtii (see paper)
47% identity, 93% coverage: 2:324/347 of query aligns to 12:339/346 of 4zgsA
- active site: S111 (≠ A101), R244 (= R234), D268 (= D259), E273 (= E264), H311 (= H296)
- binding nicotinamide-adenine-dinucleotide: Y110 (= Y100), G163 (= G153), A164 (≠ K154), I165 (= I155), D184 (= D174), C215 (= C205), P216 (= P206), L218 (≠ T208), S220 (≠ E210), T221 (= T211), S243 (≠ A233), H311 (= H296), F314 (= F299)
8grvA Dictyostelium discoideum lactate dehydrogenase (dicldha)with NAD
41% identity, 95% coverage: 1:331/347 of query aligns to 3:333/336 of 8grvA
- binding nicotinamide-adenine-dinucleotide: V106 (≠ I105), G154 (= G153), N155 (≠ K154), I156 (= I155), D176 (= D174), I177 (= I175), I178 (≠ Y176), T208 (≠ C205), P209 (= P206), T214 (= T211), V235 (≠ T232), H298 (= H296), A300 (= A298), W301 (≠ F299)
5z21B The ternary structure of d-lactate dehydrogenase from fusobacterium nucleatum with nadh and oxamate (see paper)
45% identity, 93% coverage: 2:324/347 of query aligns to 3:324/331 of 5z21B
- active site: S101 (≠ A101), R235 (= R234), D259 (= D259), E264 (= E264), H296 (= H296)
- binding 1,4-dihydronicotinamide adenine dinucleotide: Y100 (= Y100), I105 (= I105), G153 (= G153), K154 (= K154), I155 (= I155), D174 (= D174), L175 (≠ I175), P207 (= P206), T212 (= T211), T233 (= T232), G234 (≠ A233), R235 (= R234), H296 (= H296), Y299 (≠ F299)
2dldA D-lactate dehydrogenase complexed with nadh and oxamate
35% identity, 88% coverage: 12:315/347 of query aligns to 13:316/337 of 2dldA
- active site: S103 (≠ A101), R236 (= R234), D260 (= D259), E265 (= E264), H297 (= H296)
- binding 1,4-dihydronicotinamide adenine dinucleotide: T154 (= T152), G155 (= G153), H156 (≠ K154), I157 (= I155), D176 (= D174), I177 (= I175), V207 (≠ C205), P208 (= P206), N213 (≠ T211), C234 (≠ T232), S235 (≠ A233), H297 (= H296)
Sites not aligning to the query:
P30901 D-lactate dehydrogenase; D-LDH; D-specific 2-hydroxyacid dehydrogenase; EC 1.1.1.28 from Lactobacillus helveticus (Lactobacillus suntoryeus) (see paper)
35% identity, 88% coverage: 12:315/347 of query aligns to 13:316/337 of P30901
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
3kb6B Crystal structure of d-lactate dehydrogenase from aquifex aeolicus complexed with NAD and lactic acid (see paper)
35% identity, 93% coverage: 1:322/347 of query aligns to 1:320/334 of 3kb6B
- active site: S97 (≠ A101), R231 (= R234), D255 (= D259), E260 (= E264), H294 (= H296)
- binding lactic acid: F49 (= F51), S72 (= S76), V73 (≠ A77), G74 (= G78), Y96 (= Y100), R231 (= R234), H294 (= H296)
- binding nicotinamide-adenine-dinucleotide: V73 (≠ A77), Y96 (= Y100), V101 (≠ I105), G150 (= G153), R151 (≠ K154), I152 (= I155), D171 (= D174), V172 (≠ I175), P203 (= P206), T229 (= T232), A230 (= A233), R231 (= R234), H294 (= H296), A296 (= A298), Y297 (≠ F299)
P26297 D-lactate dehydrogenase; D-LDH; D-specific 2-hydroxyacid dehydrogenase; EC 1.1.1.28 from Lactobacillus delbrueckii subsp. bulgaricus (strain ATCC 11842 / DSM 20081 / BCRC 10696 / JCM 1002 / NBRC 13953 / NCIMB 11778 / NCTC 12712 / WDCM 00102 / Lb 14) (see 2 papers)
35% identity, 88% coverage: 12:315/347 of query aligns to 13:316/333 of P26297
- HI 156:157 (≠ KI 154:155) binding
- D176 (= D174) binding
- H206 (= H204) mutation to Q: Increase of activity.
- VP 207:208 (≠ CP 205:206) binding
- N213 (≠ T211) binding
- R236 (= R234) mutation to K: Decrease of activity.
- D260 (= D259) binding ; mutation to N: Decrease of activity.
- E265 (= E264) mutation to Q: Decrease of activity.
- H297 (= H296) mutation to Q: 90% loss of activity.
4prlA Crystal structure of d-lactate dehydrogenase with NAD+ from lactobacillus jensenii (see paper)
35% identity, 90% coverage: 1:313/347 of query aligns to 1:312/330 of 4prlA
- binding nicotinamide-adenine-dinucleotide: Y101 (= Y100), I106 (= I105), V154 (≠ T152), G155 (= G153), H156 (≠ K154), I157 (= I155), Y175 (= Y173), D176 (= D174), H205 (= H204), T206 (≠ C205), P207 (= P206), A233 (≠ T232), A234 (= A233), D259 (= D259), H295 (= H296), A297 (= A298)
1j49A Insights into domain closure, substrate specificity and catalysis of d-lactate dehydrogenase from lactobacillus bulgaricus (see paper)
35% identity, 88% coverage: 12:315/347 of query aligns to 13:316/332 of 1j49A
- active site: S103 (≠ A101), R236 (= R234), D260 (= D259), E265 (= E264), H297 (= H296)
- binding nicotinamide-adenine-dinucleotide: Y102 (= Y100), I107 (= I105), G153 (= G151), G155 (= G153), I157 (= I155), Y175 (= Y173), D176 (= D174), I177 (= I175), V207 (≠ C205), P208 (= P206), N213 (≠ T211), V234 (≠ T232), S235 (≠ A233), R236 (= R234), H297 (= H296), A299 (= A298), F300 (= F299)
P17584 D-2-hydroxyisocaproate dehydrogenase; D-HICDH; EC 1.1.1.- from Lacticaseibacillus paracasei (Lactobacillus paracasei) (see paper)
30% identity, 92% coverage: 1:319/347 of query aligns to 1:318/333 of P17584
1dxyA Structure of d-2-hydroxyisocaproate dehydrogenase (see paper)
30% identity, 92% coverage: 1:319/347 of query aligns to 1:318/330 of 1dxyA
- active site: S101 (≠ A101), R234 (= R234), D258 (= D259), E263 (= E262), H295 (= H296)
- binding 2-oxo-4-methylpentanoic acid: V77 (≠ A77), Y100 (= Y100), Y298 (≠ F299)
- binding nicotinamide-adenine-dinucleotide: Y100 (= Y100), G152 (= G151), G154 (= G153), H155 (≠ K154), I156 (= I155), Y174 (= Y173), D175 (= D174), P176 (≠ I175), H204 (= H204), V205 (≠ C205), P206 (= P206), N211 (≠ T211), T232 (= T232), A233 (= A233), R234 (= R234), H295 (= H296), Y298 (≠ F299)
2yq5C Crystal structure of d-isomer specific 2-hydroxyacid dehydrogenase from lactobacillus delbrueckii ssp. Bulgaricus: NAD complexed form (see paper)
33% identity, 90% coverage: 2:315/347 of query aligns to 2:316/331 of 2yq5C
- active site: S102 (≠ A101), R236 (= R234), D260 (= D259), E265 (= E264), H297 (= H296)
- binding nicotinamide-adenine-dinucleotide: Y101 (= Y100), I106 (= I105), V155 (≠ T152), G156 (= G153), H157 (≠ K154), I158 (= I155), Y176 (= Y173), D177 (= D174), V178 (≠ I175), H206 (= H204), T207 (≠ C205), P208 (= P206), A235 (= A233), R236 (= R234), H297 (= H296), F300 (= F299)
1wwkA Crystal structure of phosphoglycerate dehydrogenase from pyrococcus horikoshii ot3
36% identity, 83% coverage: 17:304/347 of query aligns to 14:286/304 of 1wwkA
- active site: S96 (≠ A101), R230 (= R234), D254 (= D259), E259 (= E264), H278 (= H296)
- binding nicotinamide-adenine-dinucleotide: V100 (≠ I105), G146 (= G151), F147 (≠ T152), G148 (= G153), R149 (≠ K154), I150 (= I155), Y168 (= Y173), D169 (= D174), P170 (≠ I175), V201 (≠ C205), P202 (= P206), T207 (= T211), T228 (= T232), S229 (≠ A233), D254 (= D259), H278 (= H296), G280 (≠ A298)
5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
35% identity, 77% coverage: 47:313/347 of query aligns to 48:308/334 of 5aovA
- active site: L100 (≠ A101), R241 (= R234), D265 (= D259), E270 (= E264), H288 (= H296)
- binding glyoxylic acid: M52 (≠ F51), L53 (≠ V52), L53 (≠ V52), Y74 (≠ R75), A75 (≠ S76), V76 (≠ A77), G77 (= G78), R241 (= R234), H288 (= H296)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (≠ A77), T104 (≠ I105), F158 (≠ T152), G159 (= G153), R160 (≠ K154), I161 (= I155), S180 (≠ D174), R181 (vs. gap), A211 (≠ H204), V212 (≠ C205), P213 (= P206), T218 (= T211), I239 (≠ T232), A240 (= A233), R241 (= R234), H288 (= H296), G290 (vs. gap)
4xkjA A novel d-lactate dehydrogenase from sporolactobacillus sp
30% identity, 91% coverage: 1:316/347 of query aligns to 1:315/332 of 4xkjA
- active site: S102 (≠ A101), R234 (= R234), D258 (= D259), E263 (= E264), H295 (= H296)
- binding nicotinamide-adenine-dinucleotide: Y101 (= Y100), V106 (≠ I105), G152 (= G151), G154 (= G153), R155 (≠ K154), I156 (= I155), D175 (= D174), I176 (= I175), R179 (≠ N178), H204 (= H204), V205 (≠ C205), P206 (= P206), T211 (= T211), A232 (≠ T232), R234 (= R234), H295 (= H296), G297 (≠ A298), F298 (= F299)
6biiA Crystal structure of pyrococcus yayanosii glyoxylate hydroxypyruvate reductase in complex with NADP and malonate (re-refinement of 5aow) (see paper)
35% identity, 77% coverage: 47:313/347 of query aligns to 47:307/332 of 6biiA
- active site: L99 (≠ A101), R240 (= R234), D264 (= D259), E269 (= E264), H287 (= H296)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V75 (≠ A77), T103 (≠ I105), G156 (= G151), F157 (≠ T152), G158 (= G153), R159 (≠ K154), I160 (= I155), A179 (≠ D174), R180 (≠ I175), S181 (≠ Y176), K183 (≠ N178), V211 (≠ C205), P212 (= P206), E216 (= E210), T217 (= T211), V238 (≠ T232), A239 (= A233), R240 (= R234), D264 (= D259), H287 (= H296), G289 (vs. gap)
3ddnB Crystal structure of hydroxypyruvic acid phosphate bound d-3- phosphoglycerate dehydrogenase in mycobacterium tuberculosis (see paper)
36% identity, 72% coverage: 56:304/347 of query aligns to 54:286/525 of 3ddnB
Sites not aligning to the query:
3dc2A Crystal structure of serine bound d-3-phosphoglycerate dehydrogenase from mycobacterium tuberculosis (see paper)
36% identity, 72% coverage: 56:304/347 of query aligns to 53:285/526 of 3dc2A
Sites not aligning to the query:
Query Sequence
>WP_013419585.1 NCBI__GCF_000166055.1:WP_013419585.1
MRIAFFSEHAFQTPFLIEANREYGHELVFFKEQLSRRTAQLAAGFVAVCPFVTDKLDAET
LRILADGGTKLIALRSAGFNNLDLAAAERLGLCVMRVPAYAPTAIAEHAVALMLALNRRI
NHAYNRVREGNFSLDHLVGFNMEGRTVGIVGTGKIGESLARIMHGFGCHILGYDIYQNPA
CIALGMKYVDLPTLLSESDIVSLHCPLTPETKHLINEETLKLMKPGSMLINTARGGLVNA
EAVIEALKRKDGLAYVGMDVYEREEGLFFSDHSSSIIQDDVFELLTTMRNVIVTGHQAFL
TREALAEIAHTTLGNVADYELGTPNPKNVLSAGVEGPGGPLHQSPRE
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory