SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013419765.1 NCBI__GCF_000166055.1:WP_013419765.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5dm3C Crystal structure of glutamine synthetase from chromohalobacter salexigens dsm 3043(csal_0679, target efi-550015) with bound adp
32% identity, 96% coverage: 14:428/433 of query aligns to 16:393/396 of 5dm3C

query
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5dm3C

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active site: E115 (= E134), E117 (= E136), E162 (= E187), E169 (= E194), H218 (= H243), R286 (= R311), E303 (= E328), R305 (= R330)
binding adenosine-5'-diphosphate: R173 (= R198), C174 (≠ H199), H220 (= H245), S222 (= S247), R301 (= R326)

5dm3A Crystal structure of glutamine synthetase from chromohalobacter salexigens dsm 3043(csal_0679, target efi-550015) with bound adp
32% identity, 96% coverage: 14:428/433 of query aligns to 18:371/374 of 5dm3A

query
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5dm3A

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active site: E107 (= E134), E109 (= E136), E146 (= E187), E150 (= E194), H199 (= H243), R265 (= R311), E282 (= E328), R284 (= R330)
binding adenosine-5'-diphosphate: I103 (≠ E130), E141 (= E182), R154 (= R198), C155 (≠ H199), H201 (= H245), S203 (= S247), R280 (= R326)

P78061 Gamma-glutamylputrescine synthetase PuuA; Gamma-Glu-Put synthetase; Glutamate--putrescine ligase; EC 6.3.1.11 from Escherichia coli (strain K12) (see paper)
30% identity, 97% coverage: 11:432/433 of query aligns to 36:471/472 of P78061

query
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P78061

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L

V

I

T

T

P

E

V

T

E

E

T

I

K

E

R

W

Y

M

L

L

V

K

N

N

H282 (= H243) mutation to N: Activity is impaired to 9% of wild-type.
R357 (= R316) mutation to Q: Activity is impaired to 3% of wild-type.

8tfkA Glutamine synthetase (see paper)
32% identity, 80% coverage: 86:430/433 of query aligns to 77:437/440 of 8tfkA

query
sites
8tfkA

V

L

P

P

W

W

E

R

P

P

G

A

-

T

-

G

-

A

V

V

A

A

I

R

T

I

L

L

G

G

R

D

T

V

E

Y

T

L

L

P

D

D

H

G

K

N

P

P

V

F

P

K

I

G

D

D

P

P

R

R

V

Y

A

V

L

L

L

K

T

T

Q

A

V

I

E

K

R

E

A

A

A

E

R

K

L

M

G

G

L

F

T

S

I

M

E

N

V

V

G

G

T

P

E
|
E

L

L

E
|
E

F

F

Y

F

L

L

L

F

-

K

-

L

-

D

-

A

-

N

-

G

D

N

P

P

E

T

T

T

K

E

R

L

P

T

K

D

D

Q

K

G

G

G

I

Y

S

F

V

D

Y

F

G

A

I

P

G

L

R

D

S

R

A

A

Q

Q

F

-

E

-

P

D

V

V

L

R

G

R

P

D

I

I

R

D

R

Y

H

A

L

L

E

E

A

H

I

M

G

G

I

F

P

Q

I

I

E

E

Q

A

S

S

N

H

P

H

E
|
E

Y

V

A

A

P

P

G

S

R

Q

V

H

E
|
E

V

I

N
x
D

I
x
F

R

R

H
x
F

A

G

G

D

A

V

L

L

T

C

G

T

A

A

D

D

R

N

V

V

L

T

F

F

R

K

T

Y

A

V

I

V

K

K

E

S

I

I

A

A

A

Y

L

H

H

K

G

G

Y

Y

L

Y

A

A

T

S

F

F

M

M

A

P

K

K

P

P

F

L

I

F

D

G

Q

V

S

N

G
|
G

S

S

G

G

F

M

H
|
H

A

S

H
x
N

Y

Q

S

S

V

L

W

F

R

K

E

D

G

G

V

K

N

N

L

V

F

F

A

Y

D

D

G

P

G

D

-

T

-

P

-

T

R

K

L

L

S

S

R

Q

F

D

G

A

R

M

S

Y

F

Y

L

I

A

G

G

G

L

L

E

L

R

K

R

H

I

I

A

R

E

E

S

F

A

T

L

A

A

V

V

T

S

N

T

P

T

V

P

V

N

N

A

S

F

Y

R

K

R
|
R

R

L

R

V

P

P

Y

G

T

Y

F
x
E

C

A

P

P

T

V

N

Y

V

I

S

S

W

W

G

S

F

A

D

Q

N

N

R
|
R

T

S

I

S

A

L

F

I

R
|
R

V

I

I

P

E

A

G

T

K

R

D

G

H

N

Q
x
G

V

T

R
|
R

I

I

E
|
E

K

L

R
|
R

D

C

G

P

S

D

A

P

D

A

A

C

N

N

P

P

Y

Y

Y

L

L

A

L

F

A

A

A

L

D

M

I

L

A

R

A

A

G

G

L

L

D

E

G

G

I

I

E

K

E

N

G

K

L

I

E

D

-

P

-

G

-

E

-

P

-

T

-

N

L

V

T

N

S

I

A

F

P

H

I

L

S

S

S

D

N

K

A

E

Y

R

E

E

Q

E

P

R

D

G

L

I

P

R

P

S

L

L

P

P

R

A

D

D

L

L

P

K

T

E

A

A

V

I

R

D

L

E

A

M

R

K

S

G

S

S

E

K

F

F

L

V

R

K

R

E

V

A

I

L

G

G

E

E

D

H

R

V

L

F

S

S

I

H

L

Y

V

L

R

C

Q

A

A

K

E

E

R

M

E

E

I

W

D

D

F

E

L

Y

E

K

A

A

Q

V

V

V

T

H

P

P

V

W

E

E

T

L

K

S

R

R

Y

Y

L

L

binding adenosine-5'-diphosphate: E128 (= E134), D194 (≠ N196), F195 (≠ I197), F197 (≠ H199), N243 (≠ H245), R312 (= R311), R317 (= R316), G325 (≠ Q324), R327 (= R326)
binding magnesium ion: E128 (= E134), E128 (= E134), E130 (= E136), E185 (= E187), E192 (= E194), E192 (= E194), H241 (= H243), E329 (= E328)
binding l-methionine-s-sulfoximine phosphate: E128 (= E134), E130 (= E136), E185 (= E187), E192 (= E194), G237 (= G239), H241 (= H243), R294 (= R293), E300 (≠ F299), R312 (= R311), R331 (= R330)

8ufjB Glutamine synthetase (see paper)
33% identity, 80% coverage: 86:430/433 of query aligns to 81:441/444 of 8ufjB

query
sites
8ufjB

V

L

P

P

W

W

E

R

P

P

G

A

-

T

-

G

-

A

V

V

A

A

I

R

T

I

L

L

G

G

R

D

T

V

E

Y

T

L

L

P

D

D

H

G

K

N

P

P

V

F

P

K

I

G

D

D

P

P

R

R

V

Y

A

V

L

L

L

K

T

T

Q

A

V

I

E

K

R

E

A

A

A

E

R

K

L

M

G

G

L

F

T

S

I

M

E

N

V

V

G

G

T

P

E
|
E

L

L

E
|
E

F

F

Y

F

L

L

L

F

D

K

P

L

E

D

T

A

K

N

R

-

P

-

K

G

D

N

K

P

G

T

I

T

S

E

V

L

Y

T

G

D

I

Q

G

G

R

G

S

Y

A

F

Q

D

F

F

E

A

P

P

V

L

-

D

L

L

G

G

P

Q

-

D

I

V

R

R

H

D

-

I

-

D

-

Y

-

A

L

L

E

E

A

H

I

M

G

G

I

F

P

Q

I

I

E

E

Q

A

S

S

N

H

P

H

E
|
E

Y

V

A

A

P

P

G

S

R

Q

V

H

E
|
E

V

I

N

D

I

F

R

R

H

F

A

G

G

D

A

V

L

L

T

C

G

T

A

A

D

D

R

N

V

V

L

T

F

F

R

K

T

Y

A

V

I

V

K

K

E

S

I

I

A

A

A

Y

L

H

H

K

G

G

Y

Y

L

Y

A

A

T

S

F

F

M

M

A

P

K

K

P

P

F

L

I

F

D

G

Q

V

S

N

G

G

S

S

G

G

F

M

H
|
H

A

S

H

N

Y

Q

S

S

V

L

W

F

R

K

E

D

G

G

V

K

N

N

L

V

F

F

A

Y

D

D

G

P

G

D

-

T

-

P

-

T

R

K

L

L

S

S

R

Q

F

D

G

A

R

M

S

Y

F

Y

L

I

A

G

G

G

L

L

E

L

R

K

R

H

I

I

A

R

E

E

S

F

A

T

L

A

A

V

V

T

S

N

T

P

T

V

P

V

N

N

A

S

F

Y

R

K

R

R

R

L

R

V

P

P

Y

G

T

Y

F

E

C

A

P

P

T

V

N

Y

V

I

S

S

W

W

G

S

F

A

D

Q

N

N

R

R

T

S

I

S

A

L

F

I

R

R

V

I

I

P

E

A

G

T

K

R

D

G

H

N

Q

G

V

T

R

R

I

I

E
|
E

K

L

R

R

D

C

G

P

S

D

A

P

D

A

A

C

N

N

P

P

Y

Y

Y

L

L

A

L

F

A

A

A

L

D

M

I

L

A

R

A

A

G

G

L

L

D

E

G

G

I

I

E

K

E

N

G

K

L

I

-

D

-

P

-

G

E

E

L

P

T

T

S

N

A

V

P

N

I

I

-

F

-

H

-

L

S

S

S

D

N

K

A

E

Y

R

E

E

Q

E

P

R

D

G

L

I

P

R

P

S

L

L

P

P

R

A

D

D

L

L

P

K

T

E

A

A

V

I

R

D

L

E

A

M

R

K

S

G

S

S

E

K

F

F

L

V

R

K

R

E

V

A

I

L

G

G

E

E

D

H

R

V

L

F

S

S

I

H

L

Y

V

L

R

C

Q

A

A

K

E

E

R

M

E

E

I

W

D

D

F

E

L

Y

E

K

A

A

Q

V

V

V

T

H

P

P

V

W

E

E

T

L

K

S

R

R

Y

Y

L

L

binding magnesium ion: E132 (= E134), E134 (= E136), E189 (= E187), E196 (= E194), H245 (= H243), E333 (= E328)

7tdpA Structure of paenibacillus polymyxa gs bound to met-sox-p-adp (transition state complex) to 1.98 angstom (see paper)
32% identity, 83% coverage: 73:433/433 of query aligns to 65:439/439 of 7tdpA

query
sites
7tdpA

D

D

V

M

H

Y

L

L

F

Y

P

P

L

D

A

L

A

D

P

T

P

W

V

V

A

V

V

F

P

P

W

W

E

S

P

D

G

R

V

V

A

A

I

R

T

L

L

I

G

C

R

D

T

I

E

Y

T

K

L

P

D

D

H

G

K

S

P

P

V

F

P

A

I

G

D

D

P

P

R

R

V

G

A

I

L

L

L

K

T

R

Q

V

V

L

E

K

R

E

A

A

A

E

R

E

L

L

G

G

L

Y

T

T

-

S

I

M

E
x
N

V

V

G
|
G

T

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

L

F

-

K

-

T

-

D

-

E

-

K

-

G

D

D

P

P

E

T

T

T

K

E

R

L

P

N

K

D

D

Q

K

G

G

G

-

Y

-

F

-

D

I

L

S

A

V

P

Y

M

G

D

I

L

G

G

R

E

S

N

A

C

Q

R

F

R

E

E

P

I

V

V

L

L

G

-

P

-

I

-

R

-

H

K

L

L

E

E

A

E

I

M

G

G

I

F

P

E

I

I

E
|
E

Q

A

S

S

N

H

P

H

E
|
E

Y

V

A

A

P

P

G

G

R

Q

V

H

E
|
E

V

I

N
x
D

I

F

R

K

H
x
Y

A

A

G

D

A

A

L

V

T

K

G

A

A

A

D

D

R

Q

V

I

V

Q

L

T

F

F

R

K

T

L

A

V

I

V

K

K

E

T

I

I

A

A

A

R

L

Q

H

H

G

G

Y

L

L

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

F

L

I

F

D

G

Q

V

S

N

G
|
G

S

S

G

G

F

M

H
|
H

A

C

H
x
N

Y

Q

S
|
S

V

L

W

F

R

K

E

D

G

N

V

E

N

N

L

V

F

F

A

Y

D

D

G

E

G

T

R

D

-

E

-

L

-

G

L

L

S

S

R

Q

F

T

G

A

R

R

S

H

F

Y

L

M

A

A

G

G

L

I

E

L

R

K

R

H

I

A

A

R

E

A

S

M

A

A

L

A

A

I

V

T

S

N

T

P

T

T

P

V

N

N

A

S

F

Y

R

K

R
|
R

R

L

R

V

P

P

Y

G

T

Y

F
x
E

C

A

P

P

T

C

N

Y

V

V

S

A

W

W

G

S

F

A

D

S

N

N

R
|
R

T

S

I

P

A

M

F

I

R
|
R

V

I

I

P

E

A

G

S

K

R

D

G

H

L

Q

S

V

T

R
|
R

I

V

E
|
E

K

V

R
|
R

D

N

G

P

S

D

A

P

D

A

A

A

N

N

P

P

Y

Y

Y

L

L

A

L

L

A

A

A

V

D

M

I

L

A

R

A

A

G

G

L

L

D

D

G

G

I

I

E

K

E

R

G

Q

L

M

E

A

L

L

T

-

S

P

A

A

P

P

I

I

S

D

S

R

N

N

A

I

Y

Y

E

V

Q

M

P

S

D

E

-

E

-

R

-

I

-

E

-

G

L

I

P

P

P

S

L

L

P

P

R

A

D

D

L

L

P

K

T

E

A

A

V

L

R

S

L

E

A

L

R

I

S

R

S

S

E

E

F

V

L

I

R

S

R

D

V

A

I

L

G

G

E

D

D

H

R

A

L

L

S

A

I

Y

L

F

V

Y

R

E

Q

L

A

K

E

E

R

I

E

E

I

W

D

D

F

M

L

Y

E

R

A

T

Q

Q

V

V

T

H

P

Q

V

W

E

E

T

R

K

D

R

Q

Y

Y

L

L

V

T

N

L

F

Y

binding adenosine-5'-diphosphate: N123 (≠ E130), G125 (= G132), E127 (= E134), E179 (= E182), D193 (≠ N196), Y196 (≠ H199), N242 (≠ H245), S244 (= S247), R316 (= R316), R326 (= R326)
binding magnesium ion: E127 (= E134), E127 (= E134), E129 (= E136), E184 (= E187), E191 (= E194), E191 (= E194), H240 (= H243), E328 (= E328)
binding l-methionine-s-sulfoximine phosphate: E127 (= E134), E129 (= E136), E184 (= E187), E191 (= E194), G236 (= G239), H240 (= H243), R293 (= R293), E299 (≠ F299), R311 (= R311), R330 (= R330)

8wwuB Glutamine synthetase
29% identity, 97% coverage: 11:430/433 of query aligns to 30:489/492 of 8wwuB

query
sites
8wwuB

G

G

T

L

H

E

T

M

I

I

V

R

L

L

G

S

V

W

G

A

D

D

V

Q

N

Y

G

G

V

L

M

L

R

R

G

G

K

K

R

S

I

L

P

T

A

V

S

A

N

S

W

L

A

K

T

S

T

A

C

F

E

K

D

E

G

G

K

S

A

E

I

V

C

A

A

I

A

G

I

P

F

F

A

F

L

F

D

D

M

L

T

V

S

S

D

S

I

M

W

V

D

F

T

N

P

L

F

F

C

T

S

T

-

A

-

G

-

D

F

F

D

E

N

D

-

E

-

L

-

S

G

G

Y

N

P

P

D

T

V

V

H

V

L

M

F

V

P

P

L

D

A

P

A

T

P

T

P

F

V

K

A

V

V

L

P

P

W

W

E

A

P

D

G

K

V

T

A

G

I

W

T

M

L

L

G

A

R

D

T

L

E

H

T

W

L

K

D

S

H

G

K

E

P

P

V

F

P

P

I

L

D

C

P

P

R

R

V

G

A

I

L

M

L

K

T

K

Q

A

V

V

E

K

R

S

A

L

A

S

R

D

L

E

G

G

L

Y

T

L

I

F

E

K

V

C

G
|
G

T

I

E
|
E

L

L

E
|
E

F

W

Y

Y

L

L

-

T

-

K

-

I

-

V

-

D

-

R

-

S

L

L

D

S

P

P

E

E

T

S

-

L

-

G

-

A

-

P

-

G

-

V

-

Q

-

P

-

D

-

A

-

I

-

Q

K

V

R

Q

P

P

K

V

D

A

K

Q

G

G

I

Y

S

S

V

V

Y

L

G

L

I

E

G

H

R

H

S

L

A

D

Q

Q

F

V

E

D

P

D

V

I

L

M

G

S

P

K

I

V

R

R

R

K

H

G

L

L

E

L

A

E

I

L

G

N

I

L

P

P

I

L

E
x
R

Q

S

S

I

N

E

P

D

E
|
E

Y

W

A

A

P

P

G

S

R

Q

V

M

E
|
E

V

T

N

T

I
x
F

R

D

H
x
V

A

M

G

E

A

G

L

L

T

E

G

A

A

A

D

D

R

A

V

A

V

L

L

L

F

I

R

K

T

S

A

A

I

I

K

K

E

Q

I

I

A

C

A

S

L

R

H

H

G

G

Y

Y

L

H

A

A

T

T

F

F

M

M

A

C

K

K

P

P

F

A

I

I

D

N

Q

G

-

F

S

F

G

A

S

S

G

G

F

W

H
|
H

A

M

H
|
H

Y

Q

S
|
S

V

L

W

V

R

D

E

K

G

D

V

T

-

R

-

K

N

N

L

L

F

F

-

I

-

P

A

S

D

E

G

G

G

E

R

V

L

L

S

S

R

P

F

L

G

G

R

R

S

A

F

Y

L

A

A

G

G

G

L

L

E

L

R

A

R

N

I

G

A

S

E

A

S

A

A

S

L

S

A

F

V

T

S

T

T

P

T

T

P

V

N

N

A

G

F

Y

R

R

R

Q

P

P

Y

Y

T

S

F

L

C

A

P

P

T

D

N

R

V

R

S

A

W

W

G

A

F

K

D

D

N

N

R
x
K

T

A

I

A

A

M

F

V

R
|
R

V

V

I

V

E

S

G

A

K

T

-

G

D

D

H

P

Q

A

V

S

R
|
R

I

I

E
|
E

K

N

R

R

D

I

G

G

S

E

A

P

D

G

A

A

N

N

P

P

Y

Y

Y

L

L

Y

L

M

A

A

A

S

D

Q

I

I

A

V

A

S

G

G

L

L

D

D

G

G

I

I

E

K

E

N

G

K

L

K

-

D

-

P

-

G

E

E

L

L

T

Q

S

E

A

S

P

P

I

Y

S

D

S

A

N

Q

A

-

Y

-

E

-

Q

-

P

-

D

-

L

V

P

P

P

M

L

L

P

P

R

T

D

T

L

L

P

A

T

E

A

A

V

L

R

D

-

A

L

L

A

E

R

H

S

D

S

S

E

E

F

L

L

F

R

R

R

S

V

C

I

F

G

G

E

D

D

T

R

F

L

I

S

K

I

Y

L

W

V

L

R

Q

Q

L

A

R

E

R

R

S

E

E

-

W

I

A

D

R

F

F

L

L

E

D

A

A

Q

E

-

G

-

A

-

E

-

A

-

E

-

P

-

T

-

G

-

A

V

V

T

T

P

Q

V

W

E

E

T

Q

K

K

R

E

Y

Y

L

F

binding phosphoaminophosphonic acid-adenylate ester: G157 (= G132), E159 (= E134), R226 (≠ E182), F241 (≠ I197), V243 (≠ H199), H290 (= H245), S292 (= S247), K360 (≠ R311), R365 (= R316), R376 (= R326)
binding magnesium ion: E159 (= E134), E238 (= E194)
binding manganese (ii) ion: E159 (= E134), E161 (= E136), E231 (= E187), E238 (= E194), H288 (= H243), E378 (= E328)

8wwvA Glutamine synthetase
29% identity, 97% coverage: 11:430/433 of query aligns to 28:487/490 of 8wwvA

query
sites
8wwvA

G

G

T

L

H

E

T

M

I

I

V

R

L

L

G

S

V

W

G

A

D

D

V

Q

N

Y

G

G

V

L

M

L

R

R

G

G

K

K

R

S

I

L

P

T

A

V

S

A

N

S

W

L

A

K

T

S

T

A

C

F

E

K

D

E

G

G

K

S

A

E

I

V

C

A

A

I

A

G

I

P

F

F

A

F

L

F

D

D

M

L

T

V

S

S

D

S

I

M

W

V

D

F

T

N

P

L

F

F

C

T

S

T

-

A

-

G

-

D

F

F

D

E

N

D

-

E

-

L

-

S

G

G

Y

N

P

P

D

T

V

V

H

V

L

M

F

V

P

P

L

D

A

P

A

T

P

T

P

F

V

K

A

V

V

L

P

P

W

W

E

A

P

D

G

K

V

T

A

G

I

W

T

M

L

L

G

A

R

D

T

L

E

H

T

W

L

K

D

S

H

G

K

E

P

P

V

F

P

P

I

L

D

C

P

P

R

R

V

G

A

I

L

M

L

K

T

K

Q

A

V

V

E

K

R

S

A

L

A

S

R

D

L

E

G

G

L

Y

T

L

I

F

E

K

V

C

G
|
G

T

I

E
|
E

L

L

E
|
E

F

W

Y

Y

L

L

-

T

-

K

-

I

-

V

-

D

-

R

-

S

L

L

D

S

P

P

E

E

T

S

-

L

-

G

-

A

-

P

-

G

-

V

-

Q

-

P

-

D

-

A

-

I

-

Q

K

V

R

Q

P

P

K

V

D

A

K

Q

G

G

I

Y

S

S

V

V

Y

L

G

L

I

E

G

H

R

H

S

L

A

D

Q

Q

F

V

E

D

P

D

V

I

L

M

G

S

P

K

I

V

R

R

R

K

H

G

L

L

E

L

A

E

I

L

G

N

I

L

P

P

I

L

E
x
R

Q

S

S

I

N

E

P

D

E
|
E

Y

W

A

A

P

P

G

S

R

Q

V

M

E
|
E

V

T

N

T

I
x
F

R
x
D

H
x
V

A

M

G

E

A

G

L

L

T

E

G

A

A

A

D

D

R

A

V

A

V

L

L

L

F

I

R

K

T

S

A

A

I

I

K

K

E

Q

I

I

A

C

A

S

L

R

H

H

G

G

Y

Y

L

H

A

A

T

T

F

F

M

M

A

C

K

K

P

P

F

A

I

I

D

N

Q

G

-

F

S

F

G
x
A

S

S

G

G

F

W

H
|
H

A

M

H
|
H

Y

Q

S
|
S

V

L

W

V

R

D

E

K

G

D

V

T

-

R

-

K

N

N

L

L

F

F

-

I

-

P

A

S

D

E

G

G

G

E

R

V

L

L

S

S

R

P

F

L

G

G

R

R

S

A

F

Y

L

A

A

G

G

G

L

L

E

L

R

A

R

N

I

G

A

S

E

A

S

A

A

S

L

S

A

F

V

T

S

T

T

P

T

T

P

V

N

N

A

G

F

Y

R

R

R
|
R

R

R

R

Q

P

P

Y

Y

T

S

F

L

C

A

P

P

T

D

N

R

V

R

S

A

W

W

G

A

F

K

D

D

N

N

R
x
K

T

A

I

A

A

M

F

V

R

R

V

V

I

V

E

S

G

A

K

T

-

G

D

D

H

P

Q

A

V

S

R
|
R

I

I

E
|
E

K

N

R

R

D

I

G

G

S

E

A

P

D

G

A

A

N

N

P

P

Y

Y

Y

L

L

Y

L

M

A

A

A

S

D

Q

I

I

A

V

A

S

G

G

L

L

D

D

G

G

I

I

E

K

E

N

G

K

L

K

-

D

-

P

-

G

E

E

L

L

T

Q

S

E

A

S

P

P

I

Y

S

D

S

A

N

Q

A

-

Y

-

E

-

Q

-

P

-

D

-

L

V

P

P

P

M

L

L

P

P

R

T

D

T

L

L

P

A

T

E

A

A

V

L

R

D

-

A

L

L

A

E

R

H

S

D

S

S

E

E

F

L

L

F

R

R

R

S

V

C

I

F

G

G

E

D

D

T

R

F

L

I

S

K

I

Y

L

W

V

L

R

Q

Q

L

A

R

E

R

R

S

E

E

-

W

I

A

D

R

F

F

L

L

E

D

A

A

Q

E

-

G

-

A

-

E

-

A

-

E

-

P

-

T

-

G

-

A

V

V

T

T

P

Q

V

W

E

E

T

Q

K

K

R

E

Y

Y

L

F

binding adenosine-5'-diphosphate: G155 (= G132), E157 (= E134), R224 (≠ E182), F239 (≠ I197), D240 (≠ R198), V241 (≠ H199), H288 (= H245), S290 (= S247), R374 (= R326), E376 (= E328)
binding magnesium ion: E157 (= E134), E236 (= E194)
binding manganese (ii) ion: E157 (= E134), E159 (= E136), E229 (= E187), E236 (= E194), H286 (= H243), E376 (= E328)
binding l-methionine-s-sulfoximine phosphate: E157 (= E134), E159 (= E136), E229 (= E187), E236 (= E194), A282 (≠ G239), H286 (= H243), R340 (= R293), K358 (≠ R311)

4lnkA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of gs-glutamate-amppcp complex (see paper)
30% identity, 98% coverage: 10:433/433 of query aligns to 15:443/443 of 4lnkA

query
sites
4lnkA

E

E

G

N

T

V

H

K

T

Y

I

I

V

R

L

L

G

Q

V

F

G

T

D

D

V

I

N

L

G

G

V

T

M

I

R

K

G

N

K

V

R

E

I

I

P

P

A

V

S

S

N

Q

W

L

A

G

T
x
K

T

A

C

L

E

D

-

N

-

K

-

V

-
x
M

-

F

D
|
D

G

G

K

S

A

S

I

I

C

E

A

G

A

-

I

-

F

F

A

V

L

R

D

I

M

E

T

E

S

S

D

D

I

M

W

Y

D

-

T

-

P

-

F

-

C

-

S

-

F

-

D

-

N

-

G

L

Y

Y

P

P

D

D

V

L

H

N

L

T

F

F

P

-

L

-

A

-

P

-

V

V

A

I

V

F

P

P

W

W

-

T

-

A

E

E

P

K

G

G

-

K

V

V

A

A

I

R

T

F

L

I

G

C

R

D

T

I

E

Y

T

N

L

P

D

D

H

G

K

T

P

P

V

F

P

E

I

G

D

D

P

P

R

R

V

N

A

N

L

L

L

K

T

R

Q

I

V

L

E

K

R

E

A

M

A

E

R

D

L

L

G

G

L

F

T

S

-

D

I

F

E

N

V

L

G

G

T

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

L

F

D

K

P

L

E

D

T

E

K

K

-

G

-

E

-

P

-

T

-

L

R

E

P

L

K

N

D

D

K

K

G

G

-

G

-

Y

-

F

-

D

I

L

S

A

V

P

Y

T

G

D

I

L

G

G

R

E

S

N

A

C

Q

R

F

R

E

D

P

I

V

V

L

L

G

-

P

-

I

-

R

-

H

E

L

L

E

E

A

E

I

M

G

G

I

F

P

E

I

I

E

E

Q

A

S

S

N

H

P

H

E
|
E

Y
x
V

A

A

P

P

G

G

R

Q

V

H

E
|
E

V

I

N

D

I
x
F

R

K

H
x
Y

A

A

G

G

A

A

L

V

T

R

G

S

A

C

D

D

R

D

V

I

V

Q

L

T

F

F

R

K

T

L

A

V

I

V

K

K

E

T

I

I

A

A

A

R

L

K

H

H

G

G

Y

L

L

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

F

L

I

F

D

G

Q

V

S
x
N

G
|
G

S

S

G
|
G

F

M

H
|
H

A

C

H
x
N

Y

L

S
|
S

V

L

W

F

R

K

E

N

G

G

V

V

N

N

L

A

F

F

A

F

D

D

G

E

G

N

-

A

-

D

-

L

R

Q

L

L

S

S

R

E

F

T

G

A

R

K

S

H

F

F

L

I

A

A

G

G

L

I

E

V

R

K

R

H

I

A

A

T

E

S

S

F

A

T

L

A

A

V

V

T

S

N

T

P

T

T

P

V

N

N

A

S

F

Y

R

K

R

R

R

L

R

V

P

P

Y

G

T

Y

F
x
E

C

A

P

P

T

C

N

Y

V

V

S

A

W

W

G

S

F

A

D

Q

N

N

R
|
R

T

S

I

P

A

L

F

I

R

R

V

I

I

P

E

A

G
x
S

K

R

D

G

H

I

Q
x
S

V

T

R
|
R

I

V

E
|
E

K

V

R
|
R

D

S

G

V

S

D

A

P

D

A

A

A

N

N

P

P

Y

Y

Y

L

L

A

L

L

A

S

A

V

D

L

I

L

A

A

G

G

L

L

D

D

G

G

I

I

E

K

E

N

G

K

L

L

E

E

L

-

T

A

S

P

A

A

P

P

I

I

S

D

S

R

N

N

A

I

Y

Y

-

V

-

M

-

S

-

K

-

E

-

R

E

M

Q

E

P

N

D

G

L

I

P

V

P

D

L

L

P

P

R

A

D

T

L

L

P

A

T

E

A

A

V

L

R

E

L

E

A

F

R

K

S

S

S

N

E

E

F

V

L

M

R

V

R

K

V

A

I

L

G

G

E

E

D

H

R

L

L

F

S

E

I

H

L

F

V

I

R

E

Q

A

A

K

E

E

R

I

E

E

I

W

D

D

F

M

L

F

E

R

A

T

Q

Q

V

V

T

H

P

P

V

W

E

E

T

R

K

E

R

Q

Y

Y

L

M

V

S

N

Q

F

Y

active site: D52 (= D42), E131 (= E134), E133 (= E136), E188 (= E187), E195 (= E194), H244 (= H243), R315 (= R311), E332 (= E328), R334 (= R330)
binding adenosine-5'-diphosphate: K43 (≠ T38), M50 (vs. gap), F198 (≠ I197), Y200 (≠ H199), N246 (≠ H245), S248 (= S247), S324 (≠ G320), S328 (≠ Q324), R330 (= R326)
binding glutamic acid: E133 (= E136), E188 (= E187), V189 (≠ Y188), N239 (≠ S238), G240 (= G239), G242 (= G241), E303 (≠ F299)
binding magnesium ion: E131 (= E134), E188 (= E187), E195 (= E194), H244 (= H243), E332 (= E328)

4lniA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of the transition state complex (see paper)
30% identity, 98% coverage: 10:433/433 of query aligns to 15:443/443 of 4lniA

query
sites
4lniA

E

E

G

N

T

V

H

K

T

Y

I

I

V

R

L

L

G

Q

V

F

G

T

D

D

V

I

N

L

G

G

V

T

M

I

R

K

G

N

K

V

R

E

I

I

P

P

A

V

S

S

N

Q

W

L

A

G

T

K

T

A

C

L

E

D

-

N

-

K

-

V

-

M

-

F

D
|
D

G

G

K

S

A

S

I

I

C

E

A

G

A

-

I

-

F

F

A

V

L

R

D

I

M

E

T

E

S

S

D

D

I

M

W

Y

D

-

T

-

P

-

F

-

C

-

S

-

F

-

D

-

N

-

G

L

Y

Y

P

P

D

D

V

L

H

N

L

T

F

F

P

-

L

-

A

-

P

-

V

V

A

I

V

F

P

P

W

W

-

T

-

A

E

E

P

K

G

G

-

K

V

V

A

A

I

R

T

F

L

I

G

C

R

D

T

I

E

Y

T

N

L

P

D

D

H

G

K

T

P

P

V

F

P

E

I

G

D

D

P

P

R

R

V

N

A

N

L

L

L

K

T

R

Q

I

V

L

E

K

R

E

A

M

A

E

R

D

L

L

G

G

L

F

T

S

-

D

I

F

E

N

V

L

G

G

T

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

L

F

D

K

P

L

E

D

T

E

K

K

-

G

-

E

-

P

-

T

-

L

R

E

P

L

K

N

D

D

K

K

G

G

-

G

-

Y

-

F

-

D

I

L

S

A

V

P

Y

T

G

D

I

L

G

G

R

E

S

N

A

C

Q

R

F

R

E

D

P

I

V

V

L

L

G

-

P

-

I

-

R

-

H

E

L

L

E

E

A

E

I

M

G

G

I

F

P

E

I

I

E
|
E

Q

A

S

S

N

H

P

H

E
|
E

Y

V

A

A

P

P

G

G

R

Q

V

H

E
|
E

V

I

N
x
D

I

F

R

K

H
x
Y

A

A

G

G

A

A

L

V

T

R

G

S

A

C

D

D

R

D

V

I

V

Q

L

T

F

F

R

K

T

L

A

V

I

V

K

K

E

T

I

I

A

A

A

R

L

K

H

H

G

G

Y

L

L

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

F

L

I

F

D

G

Q

V

S

N

G

G

S

S

G

G

F

M

H
|
H

A

C

H
x
N

Y

L

S
|
S

V

L

W

F

R

K

E

N

G

G

V

V

N

N

L

A

F

F

A

F

D

D

G

E

G

N

-

A

-

D

-

L

R

Q

L

L

S

S

R

E

F

T

G

A

R

K

S

H

F

F

L

I

A

A

G

G

L

I

E

V

R

K

R

H

I

A

A

T

E

S

S

F

A

T

L

A

A

V

V

T

S

N

T

P

T

T

P

V

N

N

A

S

F

Y

R

K

R
|
R

R

L

R

V

P

P

Y

G

T

Y

F
x
E

C

A

P

P

T

C

N

Y

V

V

S

A

W

W

G

S

F

A

D

Q

N

N

R
|
R

T

S

I

P

A

L

F

I

R
|
R

V

I

I

P

E

A

G

S

K

R

D

G

H

I

Q

S

V

T

R
|
R

I

V

E
|
E

K

V

R
|
R

D

S

G

V

S

D

A

P

D

A

A

A

N

N

P

P

Y

Y

Y

L

L

A

L

L

A

S

A

V

D

L

I

L

A

A

G

G

L

L

D

D

G

G

I

I

E

K

E

N

G

K

L

L

E

E

L

-

T

A

S

P

A

A

P

P

I

I

S

D

S

R

N

N

A

I

Y

Y

-

V

-

M

-

S

-

K

-

E

-

R

E

M

Q

E

P

N

D

G

L

I

P

V

P

D

L

L

P

P

R

A

D

T

L

L

P

A

T

E

A

A

V

L

R

E

L

E

A

F

R

K

S

S

S

N

E

E

F

V

L

M

R

V

R

K

V

A

I

L

G

G

E

E

D

H

R

L

L

F

S

E

I

H

L

F

V

I

R

E

Q

A

A

K

E

E

R

I

E

E

I

W

D

D

F

M

L

F

E

R

A

T

Q

Q

V

V

T

H

P

P

V

W

E

E

T

R

K

E

R

Q

Y

Y

L

M

V

S

N

Q

F

Y

active site: D52 (= D42), E131 (= E134), E133 (= E136), E188 (= E187), E195 (= E194), H244 (= H243), R315 (= R311), E332 (= E328), R334 (= R330)
binding adenosine-5'-diphosphate: E131 (= E134), E183 (= E182), D197 (≠ N196), Y200 (≠ H199), N246 (≠ H245), S248 (= S247), R320 (= R316), R330 (= R326)
binding magnesium ion: E131 (= E134), E131 (= E134), E133 (= E136), E188 (= E187), E195 (= E194), E195 (= E194), H244 (= H243), E332 (= E328)
binding l-methionine-s-sulfoximine phosphate: E133 (= E136), E188 (= E187), H244 (= H243), R297 (= R293), E303 (≠ F299), R315 (= R311), R334 (= R330)

P12425 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Bacillus subtilis (strain 168) (see 5 papers)
30% identity, 98% coverage: 10:433/433 of query aligns to 16:444/444 of P12425

query
sites
P12425

E

E

G

N

T

V

H

K

T

Y

I

I

V

R

L

L

G

Q

V

F

G

T

D

D

V

I

N

L

G

G

V

T

M

I

R

K

G

N

K

V

R

E

I

I

P

P

A

V

S

S

N

Q

W

L

A

G

T

K

T

A

C

L

E

D

-

N

-

K

-

V

-

M

-

F

D

D

G

G

K

S

A

S

I

I

C

E

A
x
G

A

-

I

-

F

F

A

V

L
x
R

D

I

M

E

T

E

S

S

D

D

I

M

W

Y

D

-

T

-

P

-

F

-

C

-

S

-

F

-

D

-

N

-

G

L

Y

Y

P

P

D

D

V

L

H

N

L

T

F

F

P

-

L

-

A

-

P

-

V

V

A

I

V

F

P

P

W

W

-

T

-

A

E

E

P

K

G

G

-

K

V

V

A

A

I

R

T

F

L

I

G

C

R

D

T

I

E

Y

T

N

L

P

D

D

H

G

K

T

P

P

V

F

P

E

I

G

D

D

P

P

R

R

V

N

A

N

L

L

L

K

T

R

Q

I

V

L

E

K

R

E

A

M

A

E

R

D

L

L

G

G

L

F

T

S

-

D

I

F

E

N

V

L

G

G

T

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

L

F

D

K

P

L

E

D

T

E

K

K

-

G

-

E

-

P

-

T

-

L

R

E

P

L

K

N

D

D

K

K

G

G

-

G

-

Y

-

F

-

D

I

L

S

A

V

P

Y

T

G

D

I

L

G

G

R

E

S

N

A

C

Q

R

F

R

E

D

P

I

V

V

L

L

G

-

P

-

I

-

R

-

H

E

L

L

E

E

A

E

I

M

G

G

I

F

P

E

I

I

E

E

Q

A

S

S

N

H

P

H

E
|
E

Y
x
V

A

A

P

P

G

G

R

Q

V

H

E
|
E

V

I

N

D

I

F

R

K

H

Y

A

A

G

G

A

A

L

V

T

R

G

S

A

C

D

D

R

D

V

I

V

Q

L

T

F

F

R

K

T

L

A

V

I

V

K

K

E

T

I

I

A

A

A

R

L

K

H

H

G

G

Y

L

L

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

F

L

I

F

D

G

Q

V

S

N

G
|
G

S

S

G

G

F

M

H
|
H

A

C

H

N

Y

L

S

S

V

L

W

F

R

K

E

N

G

G

V

V

N

N

L

A

F

F

A

F

D

D

G

E

G

N

-

A

-

D

-

L

R

Q

L

L

S

S

R

E

F

T

G

A

R

K

S

H

F

F

L

I

A

A

G

G

L

I

E

V

R

K

R

H

I

A

A

T

E

S

S

F

A

T

L

A

A

V

V

T

S

N

T

P

T

T

P

V

N

N

A

S

F

Y

R

K

R

R

R

L

R

V

P

P

Y
x
G

T

Y

F
x
E

C

A

P
|
P

T

C

N

Y

V

V

S

A

W

W

G

S

F

A

D

Q

N

N

R

R

T

S

I

P

A

L

F

I

R

R

V

I

I

P

E

A

G

S

K

R

D

G

H

I

Q

S

V

T

R

R

I

V

E
|
E

K

V

R

R

D

S

G

V

S

D

A

P

D

A

A

A

N

N

P

P

Y

Y

Y

L

L

A

L

L

A

S

A

V

D

L

I

L

A

A

G

G

L

L

D

D

G

G

I

I

E

K

E

N

G

K

L

L

E

E

L

-

T

A

S

P

A

A

P

P

I

I

S

D

S

R

N

N

A

I

Y

Y

-

V

-

M

-

S

-

K

-

E

-

R

E

M

Q

E

P

N

D

G

L

I

P

V

P

D

L

L

P

P

R

A

D

T

L

L

P

A

T

E

A

A

V

L

R

E

L

E

A

F

R

K

S

S

S

N

E

E

F

V

L

M

R

V

R

K

V

A

I

L

G

G

E

E

D

H

R

L

L

F

S

E

I

H

L

F

V

I

R

E

Q

A

A

K

E

E

R

I

E
|
E

I

W

D

D

F

M

L

F

E

R

A

T

Q

Q

V

V

T

H

P

P

V

W

E

E

T

R

K

E

R

Q

Y

Y

L

M

V

S

N

Q

F

Y

G59 (≠ A48) mutation to R: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion.
R62 (≠ L53) Important for inhibition by glutamine; mutation to A: Highly resistant to inhibition by glutamine and AMP. Regulation by TnrA and GlnR is abolished. Only small differences (less than 2-fold) in its steady-state kinetic constants compared with the wild-type. Similar sensitivity to Met-Sox that compared to the wild-ytpe.
E132 (= E134) binding Mg(2+)
E134 (= E136) binding Mg(2+)
E189 (= E187) binding Mg(2+)
V190 (≠ Y188) mutation to A: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant partially relieves expression of the glnRA-lacZ fusion, but has no effect on the TnrA-dependent regulation of amtB-lacZ fusion. Resistant to inhibition by MetSox.
E196 (= E194) binding Mg(2+)
G241 (= G239) binding L-glutamate
H245 (= H243) binding Mg(2+)
G302 (≠ Y297) mutation to E: Unable to form stable complex with TnrA. In the presence of glutamine, amtB-lacZ fusion is only 4-fold regulated by TnrA, whereas glnRA-lacZ fusion is derepressed. This mutant retains enzymatic specific activity with a 2-fold decrease of the affinity for glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.
E304 (≠ F299) mutation to A: Highly resistant to Met-Sox inhibition. 8- and 2-fold increase of the affinity for glutamate and ATP, respectively. Strong decrease of the affinity for ammonium.
P306 (= P301) mutation to H: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant completely derepresses glnRA-lacZ fusion, whereas amtB-lacZ fusion expression is only partially derepresses.
E333 (= E328) binding Mg(2+)
E424 (= E413) mutation to K: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion. Although it is defective in regulation, this mutant retains enzymatic specific activity and similar affinity for ATP, glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

4s0rD Structure of gs-tnra complex (see paper)
30% identity, 98% coverage: 10:433/433 of query aligns to 19:447/447 of 4s0rD

query
sites
4s0rD

E

E

G

N

T

V

H

K

T

Y

I

I

V

R

L

L

G

Q

V

F

G

T

D

D

V

I

N

L

G

G

V

T

M

I

R

K

G

N

K

V

R

E

I

I

P

P

A

V

S

S

N

Q

W

L

A

G

T

K

T

A

C

L

E

D

-

N

-

K

-

V

-

M

-

F

D
|
D

G

G

K

S

A

S

I

I

C

E

A

G

A

-

I

-

F
|
F

A
x
V

L
x
R

D
x
I

M

E

T

E

S

S

D

D

I

M

W

Y

D

-

T

-

P

-

F

-

C

-

S

-

F

-

D

-

N

-

G

L

Y

Y

P

P

D

D

V

L

H

N

L

T

F

F

P

-

L

-

A

-

P

-

V

V

A

I

V

F

P

P

W

W

-

T

-

A

E

E

P

K

G

G

-

K

V

V

A

A

I

R

T

F

L

I

G

C

R

D

T

I

E

Y

T

N

L

P

D

D

H

G

K

T

P

P

V

F

P

E

I

G

D

D

P

P

R

R

V

N

A

N

L

L

L

K

T

R

Q

I

V

L

E

K

R

E

A

M

A

E

R

D

L

L

G

G

L

F

T

S

-

D

I

F

E

N

V

L

G

G

T

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

L

F

D

K

P

L

E

D

T

E

K

K

-

G

-

E

-

P

-

T

-

L

R

E

P

L

K

N

D

D

K

K

G

G

-

G

-

Y

-

F

-
x
D

I

L

S

A

V

P

Y

T

G

D

I

L

G

G

R

E

S

N

A

C

Q

R

F

R

E

D

P

I

V

V

L

L

G

-

P

-

I

-

R

-

H

E

L

L

E

E

A

E

I

M

G

G

I

F

P

E

I

I

E

E

Q

A

S

S

N

H

P

H

E
|
E

Y

V

A

A

P

P

G

G

R

Q

V

H

E
|
E

V

I

N

D

I

F

R

K

H

Y

A

A

G

G

A

A

L

V

T

R

G

S

A

C

D

D

R

D

V

I

V

Q

L

T

F

F

R

K

T

L

A

V

I

V

K

K

E

T

I

I

A

A

A

R

L

K

H

H

G

G

Y

L

L

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

F

L

I

F

D
x
G

Q
x
V

S
x
N

G

G

S

S

G

G

F

M

H
|
H

A

C

H

N

Y

L

S

S

V

L

W

F

R

K

E

N

G

G

V

V

N

N

L

A

F

F

A

F

D

D

G

E

G

N

-

A

-

D

-

L

R

Q

L

L

S

S

R

E

F

T

G

A

R

K

S

H

F

F

L

I

A

A

G

G

L

I

E

V

R

K

R

H

I

A

A

T

E

S

S

F

A

T

L

A

A

V

V

T

S

N

T

P

T

T

P

V

N

N

A

S

F

Y

R

K

R
|
R

R

L

R

V

P

P

Y
x
G

T
x
Y

F
x
E

C

A

P

P

T

C

N

Y

V

V

S

A

W

W

G

S

F

A

D

Q

N

N

R
|
R

T

S

I

P

A

L

F

I

R

R

V

I

I

P

E

A

G

S

K

R

D

G

H

I

Q

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V

T

R

R

I

V

E
|
E

K

V

R
|
R

D

S

G

V

S

D

A

P

D

A

A

A

N

N

P

P

Y

Y

Y

L

L

A

L

L

A

S

A

V

D

L

I

L

A

A

G

G

L

L

D

D

G

G

I

I

E

K

E

N

G

K

L

L

E

E

L

-

T

A

S

P

A

A

P

P

I

I

S

D

S

R

N

N

A

I

Y
|
Y

-

V

-

M

-

S

-

K

-

E

-

R

E

M

Q

E

P

N

D

G

L

I

P

V

P

D

L

L

P

P

R

A

D

T

L

L

P

A

T

E

A

A

V

L

R

E

L

E

A

F

R

K

S

S

S

N

E

E

F

V

L

M

R

V

R

K

V

A

I

L

G

G

E

E

D

H

R

L

L

F

S

E

I
x
H

L

F

V

I

R

E

Q

A

A

K

E

E

R
x
I

E

E

I

W

D

D

F
x
M

L

F

E

R

A

T

Q

Q

V

V

T

H

P

P

V

W

E

E

T

R

K

E

R

Q

Y

Y

L

M

V

S

N

Q

F

Y

active site: D56 (= D42), E135 (= E134), E137 (= E136), E192 (= E187), E199 (= E194), H248 (= H243), R319 (= R311), E336 (= E328), R338 (= R330)
binding glutamine: E137 (= E136), E192 (= E187), R301 (= R293), E307 (≠ F299)
binding magnesium ion: I66 (≠ D54), E135 (= E134), E135 (= E134), E199 (= E194), H248 (= H243), H248 (= H243), E336 (= E328), H419 (≠ I405)
binding : F63 (= F51), V64 (≠ A52), R65 (≠ L53), I66 (≠ D54), D161 (vs. gap), G241 (≠ D236), V242 (≠ Q237), N243 (≠ S238), G305 (≠ Y297), Y306 (≠ T298), Y376 (= Y369), I426 (≠ R412), M430 (≠ F416)

P9WN37 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
35% identity, 76% coverage: 103:430/433 of query aligns to 101:444/446 of P9WN37

query
sites
P9WN37

D

D

H

G

K

S

P

P

V

S

P

W

I

A

D

D

P

P

R

R

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H

A

V

L

L

L

R

T

R

Q

Q

V

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E

T

R

K

A

A

A

G

R

E

L

L

G

G

L

F

T

S

I

C

E

Y

V

V

G

H

T

P

E

E

L

I

E

E

F

F

Y

F

L

L

D

K

P

P

E

-

T

-

K

-

R

G

P

P

K

E

D

D

K

G

G

S

I

V

S

P

V

V

-

P

-

V

-

D

-

N

Y

A

G

G

I

Y

G

F

R

D

S

Q

A

A

Q

V

F

H

E

D

P

S

V

A

L

L

G

N

P

-

I

F

R

R

H

H

-

A

-

I

-

D

-

A

L

L

E

E

A

F

I

M

G

G

I

I

P

S

I

V

E

E

Q

F

S

S

N

H

P

H

E

E

Y

G

A

A

P

P

G

G

R

Q

V

Q

E

E

V

I

N

D

I

L

R

R

H

F

A

A

G

D

A

A

L

L

T

S

G

M

A

A

D

D

R

N

V

V

V

M

L

T

F

F

R

R

T

Y

A

V

I

I

K

K

E

E

I

V

A

A

A

L

L

E

H

E

G

G

Y

A

L

R

A

A

T

S

F

F

M

M

A

P

K

K

P

P

F

F

I

G

D

Q

Q

H

S

P

G

G

S

S

G

A

F

M

H

H

A

T

H

H

Y

M

S

S

V

L

W

F

R

E

E

G

G

D

V

V

N

N

L

A

F

F

A

H

-

S

-

A

-

D

D

D

G

P

G

L

R

Q

L

L

S

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R

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F

V

G

G

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K

S

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F

F

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I

A

A

G

G

L

I

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L

R

E

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H

I

A

A

C

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I

A

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L

A

A

V

V

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N

T

Q

T

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N

N

A

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F

Y

R

K

R

R

R

L

R

V

P

Q

Y

G

T

G

F

E

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A

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P

T

T

N

A

V

A

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S

W

W

G

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F

A

D

A

N

N

R

R

T

S

I

A

A

L

F

V

R

R

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V

I

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E

M

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Y

K

T

D

P

H

H

Q

K

V

T

-

S

-

R

R

R

I

V

E

E

K

V

R

R

D

S

G

P

S

D

A

S

D

A

A

C

N

N

P

P

Y

Y

Y

L

L

T

L

F

A

A

A

V

D

L

I

L

A

A

G

G

L

L

D

R

G

G

I

V

E

E

E
x
K

G

G

L

Y

E

V

L

L

T

-

S

G

A

P

P

Q

I

A

S

E

S

D

N

N

A

V

Y

W

E

-

Q

-

P

-

D

D

L

L

P

T

P

P

-

E

-

R

-

A

-

M

-

G

-

Y

-

R

-

E

L

L

P

P

R

S

D

S

L

L

P

D

T

S

A

A

V

L

R

R

L

A

A

M

R

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A

S

S

E

E

F

L

L

V

R

A

R

E

V

A

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G

G

E

E

D

H

R

V

L

F

S

D

I

F

L

F

V

L

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R

Q

N

A

K

E

R

R

T

E

E

I

W

D

A

F

N

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Y

E

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A

S

Q

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V

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T

T

P

P

V

Y

E

E

T

L

K

R

R

T

Y

Y

L

L

K363 (≠ E355) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

7tfaB Glutamine synthetase (see paper)
32% identity, 83% coverage: 73:433/433 of query aligns to 65:441/441 of 7tfaB

query
sites
7tfaB

D

D

V

M

H

Y

L

L

F

Y

P

P

L

D

A

L

A

D

P

T

P

W

V

V

A

V

V

F

P

P

W

W

E

V

P

T

G

S

-

D

-

R

V

V

A

A

I

R

T

L

L

I

G

C

R

D

T

I

E

Y

T

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D

D

H

G

K

S

P

P

V

F

P

A

I

G

D

D

P

P

R

R

V

G

A

I

L

L

L

K

T

R

Q

V

V

L

E

K

R

E

A

A

A

E

R

E

L

L

G

G

L

Y

T

T

-

S

I

M

E

N

V

V

G

G

T

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

L

F

-

K

-

T

-

D

-

E

-

K

-

G

D

D

P

P

E

T

T

T

K

E

R

L

P

N

K

D

D

Q

K

G

G

G

-
x
Y

-

F

-

D

I

L

S

A

V

P

Y

M

G

D

I

L

G

G

R

E

S

N

A

C

Q

R

F

R

E

E

P

I

V

V

L

L

G

-

P

-

I

-

R

-

H

K

L

L

E

E

A

E

I

M

G

G

I

F

P

E

I

I

E

E

Q

A

S

S

N

H

P

H

E
|
E

Y
x
V

A

A

P

P

G

G

R

Q

V

H

E
|
E

V

I

N

D

I

F

R

K

H

Y

A

A

G

D

A

A

L

V

T

K

G

A

A

A

D

D

R

Q

V

I

V

Q

L

T

F

F

R

K

T

L

A

V

I

V

K

K

E

T

I

I

A

A

A

R

L

Q

H

H

G

G

Y

L

L

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

F

L

I

F

D

G

Q

V

S
x
N

G
|
G

S

S

G

G

F

M

H
|
H

A

C

H

N

Y

Q

S

S

V

L

W

F

R

K

E

D

G

N

V

E

N

N

L

V

F

F

A

Y

D

D

G

E

G

T

R

D

-

E

-

L

-

G

L

L

S

S

R

Q

F

T

G

A

R

R

S

H

F

Y

L

M

A

A

G

G

L

I

E

L

R

K

R

H

I

A

A

R

E

A

S

M

A

A

L

A

A

I

V

T

S

N

T

P

T

T

P

V

N

N

A

S

F

Y

R

K

R
|
R

R

L

R

V

P

P

Y
x
G

T
x
Y

F
x
E

C

A

P

P

T

C

N

Y

V

V

S

A

W

W

G

S

F

A

D

S

N

N

R
|
R

T

S

I

P

A

M

F

I

R

R

V

I

I

P

E

A

G

S

K

R

D

G

H

L

Q

S

V

T

R

R

I

V

E
|
E

K

V

R

R

D

N

G

P

S

D

A

P

D

A

A

A

N

N

P

P

Y

Y

Y

L

L

A

L

L

A

A

A

V

D

M

I

L

A

R

A

A

G

G

L

L

D

D

G

G

I

I

E

K

E

R

G

Q

L

M

E

A

L

L

T

-

S

P

A

A

P

P

I

I

S

D

S

R

N

N

A

I

Y

Y

E

V

Q

M

P

S

D

E

-

E

-

R

-

I

-

E

-

G

L

I

P

P

P

S

L

L

P

P

R

A

D

D

L

L

P

K

T

E

A

A

V

L

R

S

L

E

A

L

R

I

S

R

S

S

E

E

F

V

L

I

R

S

R

D

V

A

I

L

G

G

E

D

D

H

R

A

L

L

S

A

I

Y

L

F

V

Y

R

E

Q

L

A

K

E

E

R

I

E

E

I

W

D

D

F
x
M

L

Y

E

R

A

T

Q

Q

V

V

T

H

P

Q

V

W

E

E

T

R

K

D

R

Q

Y

Y

L

L

V

T

N

L

F

Y

binding glutamine: E131 (= E136), Y153 (vs. gap), E186 (= E187), G238 (= G239), H242 (= H243), R295 (= R293), E301 (≠ F299)
binding magnesium ion: E129 (= E134), E131 (= E136), E186 (= E187), E193 (= E194), H242 (= H243), E330 (= E328)
binding : V187 (≠ Y188), N237 (≠ S238), G299 (≠ Y297), Y300 (≠ T298), R313 (= R311), M424 (≠ F416)

Sites not aligning to the query:

binding : 58, 60

A0R083 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
33% identity, 76% coverage: 103:430/433 of query aligns to 101:444/446 of A0R083

query
sites
A0R083

D

D

H

G

K

S

P

P

V

S

P

W

I

A

D

D

P

S

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H

A

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L

L

R

T

R

Q

Q

V

L

E

A

R

K

A

A

A

S

R

D

L

L

G

G

L

F

T

T

I

C

E

Y

V

V

G

H

T

P

E

E

L

I

E

E

F

F

Y

F

L

L

D

K

P

P

E

G

T

P

K

N

-

D

-

G

-

T

-

P

R

E

P

P

K

A

D

D

K

N

G

G

I

-

S

-

V

-

Y

-

G

-

I

-

G

G

R

Y

S

F

A

D

Q

Q

F

A

E

V

P

H

V

D

L

A

G

A

P

P

-

N

I

F

R

R

H

H

-

A

-

I

-

E

-

A

L

L

E

E

A

Q

I

M

G

G

I

I

P

S

I

V

E

E

Q

F

S

S

N

H

P

H

E

E

Y

G

A

A

P

P

G

G

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Q

E

E

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I

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D

I

L

R

R

H

Y

A

A

G

D

A

A

L

L

T

S

G

M

A

A

D

D

R

N

V

V

V

M

L

T

F

F

R

R

T

Y

A

L

I

V

K

K

E

E

I

V

A

A

A

L

L

A

H

D

G

G

Y

V

L

R

A

A

T

S

F

F

M

M

A

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K

P

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F

I

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G

G

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G

A

F

M

H

H

A

T

H

H

Y

M

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S

V

L

W

F

R

E

E

G

G

D

V

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N

N

L

A

F

F

A

H

-

S

-

P

-

D

D

D

G

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L

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F

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A

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F

F

L

I

A

A

G

G

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E

L

R

E

R

H

I

A

A

N

E

E

S

I

A

S

L

A

A

V

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T

S

N

T

Q

T

W

P

V

N

N

A

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F

Y

R

K

R

R

R

L

R

V

P

H

Y

G

T

G

F

E

C

A

P

P

T

T

N

A

V

A

S

S

W

W

G

G

F

A

D

A

N

N

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I

A

A

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F

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G

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H

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Q

K

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-

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I

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E

E

K

V

R

R

D

S

G

P

S

D

A

S

D

A

A

C

N

N

P

P

Y

Y

Y

L

L

T

L

F

A

A

A

V

D

L

I

L

A

A

G

G

L

L

D

R

G

G

I

V

E

E

E
x
K

G

G

L

Y

-

V

-

L

-

G

-

P

-

Q

-

A

-

E

-

D

-

N

-

V

-

W

E

S

L

L

T

T

S

Q

A

E

P

E

I

R

S

R

S

A

N

M

A

G

Y

Y

E

R

Q

E

P

-

D

-

L

-

P

-

L

L

P

P

R

T

D

S

L

L

P

G

T

N

A

A

V

L

R

E

L

S

A

M

R

E

S

N

S

S

E

E

F

L

L

V

R

A

R

E

V

A

I

L

G

G

E

E

D

H

R

V

L

F

S

D

I

Y

L

F

V

L

R

R

Q

N

A

K

E

R

R

S

E

E

I

W

D

E

F

N

L

Y

E

R

A

S

Q

H

V

V

T

T

P

P

V

Y

E

E

T

L

K

K

R

N

Y

Y

L

L

K363 (≠ E355) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

8oooA Glutamine synthetase from methanothermococcus thermolithotrophicus in complex with 2-oxoglutarate and mgatp at 2.15 a resolution (see paper)
30% identity, 83% coverage: 73:430/433 of query aligns to 71:445/447 of 8oooA

query
sites
8oooA

D

D

V

M

H

M

L

L

F

K

P

P

L

D

A

L

A

S

P

T

P

F

V

S

A

V

V

L

P

P

W

W

E
x
R

P

P

G

S

-

E

-

K

-

S

V
|
V

A

A

I

R

T

V

L

I

G

C

R

D

T

V

E

Y

T

T

L

T

D

K

H

G

K

K

P

P

V

F

P

E

I

G

D

D

P

P

R

R

V

G

A

C

L

L

L

K

T

R

Q

V

V

M

E

E

R

E

A

F

A

K

R

K

-

E

L

F

G

N

L

G

T

E

I

Y

E

F

V

V

G

G

T

P

E
|
E

L

P

E

E

F

F

Y

F

L

L

-

K

-

D

-

P

-

H

D

N

P

P

E

H

T

K

K

Y

R

I

P

P

K

A

D

D

K

D

G

G

I

-

S

G

V

Y

Y

F

G

D

I

L

G

E

R

P

S

M

A

D

Q

E

F

A

E
x
P

P

D

V

I

L
x
R

G
x
R

P

D

I

I

R

V

R

F

H

A

L

L

E

E

A

N

I

L

G

G

I

F

P

H

I

V

E
|
E

Q

A

S
|
S

N

H

P

H

E

E

Y

V

A

A

P

P

G

G

R

Q

V

H

E

E

V

V

N

D

I
x
F

R
x
K

H
x
F

A

D

G

D

A

A

L

L

T

K

G

T

A

A

D

D

R

S

V

V

V

I

L

T

F

F

R

K

T

T

A

T

I

I

K

K

E

T

I

I

A

A

A

E

L

Q

H

H

G

G

Y

L

L

K

A

A

T

T

F

F

M

M

A

P

K

K

P

P

F

F

I

F

D

G

Q

M

S

N

G

G

S

S

G

G

F

M

H

H

A

C

H
|
H

Y

Q

S
|
S

V

I

W

W

R

L

E

N

G

G

V

E

N

P

L

S

F

F

A

Y

D

D

G

E

G

N

-

A

-

P

-

Y

R

Q

L

L

S

S

R

E

F

T

G

C

R

M

S

N

F

Y

L

V

A

A

G

G

L

I

E

L

R

K

R

H

I

A

A

K

E

A

S

I

A

V

L

A

A

I

V

T

S

N

T

P

T

T

P

V

N

N

A

S

F

Y

R

K

R

R

R

L

R

V

P

P

Y

G

T

Y

F

E

C

A

P

P

T

V

N

N

V

I

S

A

W

W

G

A

F

N

D

S

N

N

R

R

T

S

I

A

A

I

F

I

R
|
R

V

V

I

P

E

A

G

A

K

R

D

G

H

K

Q

G

V

T

R
|
R

I

I

E

E

K

F

R

R

D

A

G

P

S

D

A

P

D

S

A

C

N

N

P

P

Y

Y

Y

L

L

A

L

F

A

T

A

V

D

M

I

L

A

A

G

G

L

L

D

D

G

G

I

V

E

K

E

N

G

K

L

L

E

D

L

-

T

A

S

P

A

E

P

P

I

V

S

E

S

R

N

N

A

I

Y

F

E

A

Q

M

P

S

D

E

-

A

-

E

-

K

-

E

-

L

-

G

L

I

P

E

P

S

L

V

P

P

R

A

D

N

L

L

P

K

T

A

A

A

V

L

R

D

L

E

A

L

R

E

S

N

E

D

F

V

L

L

R

K

R

N

V

A

I

L

G

G

E

K

D

H

R

I

L

F

S

E

I

S

L

F

V

L

R

E

Q

I

A

K

E

N

R

A

E

E

I

W

D

D

F

S

L

F

E

R

A

T

Q

S

V

V

T

T

P

D

V

W

E

E

T

T

K

T

R

A

Y

Y

L

L

binding 2-oxoglutaric acid: R87 (≠ E89), V93 (= V92), P170 (≠ E164), R173 (≠ L167), R174 (≠ G168), S190 (= S184)
binding adenosine-5'-triphosphate: E136 (= E134), E188 (= E182), F203 (≠ I197), K204 (≠ R198), F205 (≠ H199), H251 (= H245), S253 (= S247), R325 (= R316), R335 (= R326)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 17, 19, 33

8ooqB Glutamine synthetase from Methanothermococcus thermolithotrophicus (see paper)
30% identity, 83% coverage: 73:430/433 of query aligns to 70:444/446 of 8ooqB

query
sites
8ooqB

D

D

V

M

H

M

L

L

F

K

P

P

L

D

A

L

A

S

P

T

P

F

V

S

A

V

V

L

P

P

W

W

E
x
R

P

P

G

S

-

E

-

K

-

S

V
|
V

A

A

I

R

T

V

L

I

G

C

R

D

T

V

E

Y

T

T

L

T

D

K

H

G

K

K

P

P

V

F

P

E

I

G

D

D

P

P

R

R

V

G

A

C

L

L

L

K

T

R

Q

V

V

M

E

E

R

E

A

F

A

K

R

K

-

E

L

F

G

N

L

G

T

E

I

Y

E

F

V

V

G

G

T

P

E

E

L

P

E
|
E

F

F

Y

F

L

L

-

K

-

D

-

P

-

H

D

N

P

P

E

H

T

K

K

Y

R

I

P

P

K

A

D

D

K

D

G

G

I

-

S

G

V

Y

Y

F

G

D

I

L

G

E

R

P

S

M

A

D

Q

E

F

A

E
x
P

P

D

V

I

L
x
R

G
x
R

P

D

I

I

R

V

R

F

H

A

L

L

E

E

A

N

I

L

G

G

I

F

P

H

I

V

E

E

Q

A

S
|
S

N

H

P

H

E
|
E

Y

V

A

A

P

P

G

G

R

Q

V

H

E
|
E

V

V

N

D

I

F

R

K

H

F

A

D

G

D

A

A

L

L

T

K

G

T

A

A

D

D

R

S

V

V

V

I

L

T

F

F

R

K

T

T

A

T

I

I

K

K

E

T

I

I

A

A

A

E

L

Q

H

H

G

G

Y

L

L

K

A

A

T

T

F

F

M

M

A

P

K

K

P

P

F

F

I

F

D

G

Q

M

S

N

G

G

S

S

G

G

F

M

H

H

A

C

H

H

Y

Q

S

S

V

I

W

W

R

L

E

N

G

G

V

E

N

P

L

S

F

F

A

Y

D

D

G

E

G

N

-

A

-

P

-

Y

R

Q

L

L

S

S

R

E

F

T

G

C

R

M

S

N

F

Y

L

V

A

A

G

G

L

I

E

L

R

K

R

H

I

A

A

K

E

A

S

I

A

V

L

A

A

I

V

T

S

N

T

P

T

T

P

V

N

N

A

S

F

Y

R

K

R

R

R

L

R

V

P

P

Y

G

T

Y

F

E

C

A

P

P

T

V

N

N

V

I

S

A

W

W

G

A

F

N

D

S

N

N

R

R

T

S

I

A

A

I

F

I

R

R

V

V

I

P

E

A

G

A

K

R

D

G

H

K

Q

G

V

T

R

R

I

I

E

E

K

F

R

R

D

A

G

P

S

D

A

P

D

S

A

C

N

N

P

P

Y

Y

Y

L

L

A

L

F

A

T

A

V

D

M

I

L

A

A

G

G

L

L

D

D

G

G

I

V

E

K

E

N

G

K

L

L

E

D

L

-

T

A

S

P

A

E

P

P

I

V

S

E

S

R

N

N

A

I

Y

F

E

A

Q

M

P

S

D

E

-

A

-

E

-

K

-

E

-

L

-

G

L

I

P

E

P

S

L

V

P

P

R

A

D

N

L

L

P

K

T

A

A

A

V

L

R

D

L

E

A

L

R

E

S

N

E

D

F

V

L

L

R

K

R

N

V

A

I

L

G

G

E

K

D

H

R

I

L

F

S

E

I

S

L

F

V

L

R

E

Q

I

A

K

E

N

R

A

E

E

I

W

D

D

F

S

L

F

E

R

A

T

Q

S

V

V

T

T

P

D

V

W

E

E

T

T

K

T

R

A

Y

Y

L

L

binding 2-oxoglutaric acid: R86 (≠ E89), V92 (= V92), P169 (≠ E164), R172 (≠ L167), R173 (≠ G168), S189 (= S184)
binding magnesium ion: E137 (= E136), E192 (= E187), E199 (= E194)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 16, 18, 32

8oozA Glutamine synthetase (see paper)
30% identity, 80% coverage: 86:433/433 of query aligns to 71:430/430 of 8oozA

query
sites
8oozA

V

L

P

P

W

W

E

S

P

T

G

A

V

E

A

A

I

R

T

L

L

I

G

C

R

D

T

I

E

E

T

L

L

P

D

D

H

G

K

K

P

P

V

F

P

M

I

G

D

C

P

P

R

R

V

Q

A

V

L

L

L

K

T

K

Q

N

V

M

E

E

R

E

A

A

R

K

L

L

G

G

L

Y

T

V

I

M

E

N

V

T

G
|
G

T

P

E
|
E

L

M

E

E

F

F

Y

F

L

L

L

F

D

K

P

R

E

Q

-

D

-

G

-

M

-

P

T

T

K

N

R

I

P

P

K

Q

D

D

K

R

G

G

I

-

S

G

V

Y

Y

F

G

D

I

L

G

A

R

P

S

I

A

D

Q

L

F

A

E

E

P

E

V

I

L

K

G

R

P

E

I

I

R

V

R

L

H

V

L

L

E

E

A

E

I

M

G

G

I

F

P

E

I

V

E
|
E

Q

A

S

A

N

H

P

H

E

E

Y

V

A

A

P

F

G

G

R

Q

V

H

E

E

V

I

N

D

I
x
F

R
x
K

H
x
Y

A

D

G

N

A

A

L

L

T

A

G

T

A

A

D

D

R

N

V

V

V

I

L

T

F

L

R

K

T

Y

A

V

I

A

K

K

E

T

I

L

A

A

A

L

L

Q

H

H

G

G

Y

L

L

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

F

I

I

F

D

G

Q

V

S

N

G

G

S

S

G

G

F

M

H
|
H

A

T

H
x
N

Y

T

S
|
S

V

L

W

F

R

K

E

D

G

G

V

K

N

N

L

A

F

F

A

Y

D

D

-

P

-

D

-

A

G

P

G

D

R

Q

L

I

S

S

R

D

F

T

G

L

R

R

S

Y

F

F

L

V

A

G

G

G

L

V

E

L

R

K

R

H

I

I

A

-

E

-

S

R

A

A

L

I

A

T

V

A

S

I

T

T

T

N

P

P

-

L

-

V

N

N

A

S

F

Y

R

K

R

R

R

L

R

V

P

P

Y

G

T

Y

F

E

C

A

P

P

T

V

N

Y

V

I

S

T

W

W

G

S

F

G

D

P

N

N

R

R

T

S

I

S

A

L

F

I

R

R

V

V

I

P

E

A

G

P

K

R

D

G

H

N

Q
x
S

V

T

R
|
R

I

I

E
|
E

K

I

R

R

D

S

G

P

S

D

A

P

D

S

A

C

N

N

P

P

Y

Y

Y

L

L

A

L

F

A

A

D

I

I

L

A

A

G

G

L

L

D

D

G

G

I

V

E

K

-

N

-

K

-

I

-

E

-

P

E

E

G

R

L

V

E

E

L

K

T

N

S

I

A

Y

P

K

I

L

S

T

S

E

N

E

A

E

Y

R

E

E

Q

K

P

L

D

G

L

I

P

G

P

M

L

L

P

P

R

G

D

T

L

L

P

K

T

E

A

A

V

I

R

E

L

C

A

F

R

K

S

E

S

D

E

E

F

L

L

L

R

V

R

S

V

A

I

L

G

G

E

E

D

H

R

V

L

S

S

Q

I

S

L

I

V

I

R

N

Q

V

A

A

E

M

R

A

E

D

I

W

D

D

F

S

L

Y

E

R

A

T

Q

Q

V

V

T

H

P

Q

V

W

E

E

T

L

K

D

R

R

Y

Y

L

L

V

Q

N

T

F

Y

binding adenosine-5'-triphosphate: G117 (= G132), E170 (= E182), F185 (≠ I197), K186 (≠ R198), Y187 (≠ H199), N233 (≠ H245), S235 (= S247), S315 (≠ Q324), R317 (= R326)
binding magnesium ion: E119 (= E134), H231 (= H243), E319 (= E328)

7tenA Glutamine synthetase (see paper)
31% identity, 83% coverage: 73:430/433 of query aligns to 65:439/442 of 7tenA

query
sites
7tenA

D

D

V

M

H

Y

L

L

F

F

P

P

L

D

A

L

A

D

P

T

P

W

V

V

A

V

V

F

P

P

W

W

-

T

-

A

E

E

P

K

G

G

-

K

V

V

A

A

I

R

T

M

L

I

G

C

R

D

T

I

E

Y

T

N

L

P

D

D

H

M

K

T

P

P

V

F

P

A

I

G

D

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binding adenosine-5'-diphosphate: G128 (= G132), E130 (= E134), E182 (= E182), D196 (≠ N196), F197 (≠ I197), K198 (≠ R198), Y199 (≠ H199), N245 (≠ H245), S247 (= S247), R319 (= R316), S327 (≠ Q324), R329 (= R326)
binding l-methionine-s-sulfoximine phosphate: E130 (= E134), E132 (= E136), E187 (= E187), E194 (= E194), N238 (≠ S238), G239 (= G239), H243 (= H243), R296 (= R293), E302 (≠ F299), R314 (= R311), R333 (= R330)

7tf9S L. Monocytogenes gs(14)-q-glnr peptide (see paper)
31% identity, 83% coverage: 73:430/433 of query aligns to 66:440/443 of 7tf9S

query
sites
7tf9S

D

D

V

M

H

Y

L

L

F

F

P

P

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A

L

A

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W

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binding glutamine: E133 (= E136), Y155 (vs. gap), E188 (= E187), G240 (= G239), G242 (= G241), R297 (= R293), E303 (≠ F299)
binding magnesium ion: E131 (= E134), E133 (= E136), E188 (= E187), E195 (= E194), H244 (= H243), E332 (= E328)
binding : E418 (≠ R408), I422 (≠ R412), M426 (≠ F416)

Sites not aligning to the query:

binding : 59, 60

Query Sequence

>WP_013419765.1 NCBI__GCF_000166055.1:WP_013419765.1
MSGSLLPLPEGTHTIVLGVGDVNGVMRGKRIPASNWATTCEDGKAICAAIFALDMTSDIW
DTPFCSFDNGYPDVHLFPLAAPPVAVPWEPGVAITLGRTETLDHKPVPIDPRVALLTQVE
RAARLGLTIEVGTELEFYLLDPETKRPKDKGISVYGIGRSAQFEPVLGPIRRHLEAIGIP
IEQSNPEYAPGRVEVNIRHAGALTGADRVVLFRTAIKEIAALHGYLATFMAKPFIDQSGS
GFHAHYSVWREGVNLFADGGRLSRFGRSFLAGLERRIAESALAVSTTPNAFRRRRPYTFC
PTNVSWGFDNRTIAFRVIEGKDHQVRIEKRDGSADANPYYLLAADIAAGLDGIEEGLELT
SAPISSNAYEQPDLPPLPRDLPTAVRLARSSEFLRRVIGEDRLSILVRQAEREIDFLEAQ
VTPVETKRYLVNF

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory