SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013419965.1 NCBI__GCF_000166055.1:WP_013419965.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
63% identity, 96% coverage: 13:322/322 of query aligns to 3:309/310 of P9WP55

query
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P9WP55

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K44 (= K56) modified: N6-(pyridoxal phosphate)lysine
N74 (= N87) binding pyridoxal 5'-phosphate
GTGGT 178:182 (= GTGGT 191:195) binding pyridoxal 5'-phosphate
S266 (= S279) binding pyridoxal 5'-phosphate

2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
64% identity, 95% coverage: 13:318/322 of query aligns to 3:305/306 of 2q3dA

query
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2q3dA

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active site: K44 (= K56), S266 (= S279), P293 (= P306)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K56), T71 (= T84), S72 (= S85), N74 (= N87), T75 (= T88), Q144 (= Q157), V177 (≠ I190), G178 (= G191), T179 (= T192), G180 (= G193), T182 (= T195), G222 (= G235), I223 (= I236), S266 (= S279), P293 (= P306), D294 (≠ S307)

4lmaA Crystal structure analysis of o-acetylserine sulfhydrylase cysk1 from microcystis aeruginosa 7806 (see paper)
59% identity, 96% coverage: 12:321/322 of query aligns to 2:311/318 of 4lmaA

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4lmaA

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active site: K46 (= K56), S269 (= S279)
binding pyridoxal-5'-phosphate: K46 (= K56), N77 (= N87), G181 (= G191), T182 (= T192), G183 (= G193), T185 (= T195), G225 (= G235), S269 (= S279), P296 (= P306)

3zeiA Structure of the mycobacterium tuberculosis o-acetylserine sulfhydrylase (oass) cysk1 in complex with a small molecule inhibitor (see paper)
63% identity, 93% coverage: 13:313/322 of query aligns to 3:300/300 of 3zeiA

query
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3zeiA

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active site: K44 (= K56), S266 (= S279), P293 (= P306)
binding 3-[(Z)-[(5Z)-5-[[2-(2-hydroxy-2-oxoethyloxy)phenyl]methylidene]-3-methyl-4-oxidanylidene-1,3-thiazolidin-2-ylidene]amino]benzoic acid: T71 (= T84), S72 (= S85), I126 (≠ V139), Q144 (= Q157), F145 (= F158), K215 (≠ P228), G222 (= G235), A225 (= A238), F227 (= F240)
binding pyridoxal-5'-phosphate: K44 (= K56), N74 (= N87), V177 (≠ I190), G178 (= G191), T179 (= T192), G180 (= G193), T182 (= T195), G222 (= G235), S266 (= S279), P293 (= P306), D294 (≠ S307)

2q3cA 2.1 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) holoenzyme from mycobacterium tuberculosis in complex with the inhibitory peptide dfsi (see paper)
63% identity, 93% coverage: 13:313/322 of query aligns to 3:300/300 of 2q3cA

query
sites
2q3cA

I

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Y

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G

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C

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R

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A

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R

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|
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F

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N

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A

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P

E

A

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I

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H

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A

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I

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x
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G

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A

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F

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P

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E

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A

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A

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A

A

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N

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active site: K44 (= K56), S266 (= S279), P293 (= P306)
binding : T71 (= T84), S72 (= S85), G73 (= G86), T75 (= T88), M122 (= M135), Q144 (= Q157), K215 (≠ P228), G222 (= G235), A225 (= A238)

4lmbA Crystal structure analysis of o-acetylserine sulfhydrylase cysk2 complexed with cystine from microcystis aeruginosa 7806 (see paper)
61% identity, 95% coverage: 13:319/322 of query aligns to 3:309/310 of 4lmbA

query
sites
4lmbA

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x
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A

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A

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N

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active site: K46 (= K56), S269 (= S279)
binding cysteine: K46 (= K56), T74 (= T84), S75 (= S85), N77 (= N87), T78 (= T88), M101 (= M111), M125 (= M135), M125 (= M135), Q147 (= Q157), F148 (= F158), Q224 (= Q234), G225 (= G235), G225 (= G235), I226 (= I236), A228 (= A238)
binding pyridoxal-5'-phosphate: K46 (= K56), N77 (= N87), V180 (≠ I190), G181 (= G191), T182 (= T192), G183 (= G193), T185 (= T195), G225 (= G235), S269 (= S279), P296 (= P306)

4aecA Crystal structure of the arabidopsis thaliana o-acetyl-serine-(thiol)- lyasE C (see paper)
59% identity, 96% coverage: 13:321/322 of query aligns to 13:319/323 of 4aecA

query
sites
4aecA

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N

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A

A

I

A

A

A

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I

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K

R

R

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P

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E

N

N

A

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G

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I

active site: K54 (= K56), S277 (= S279)
binding pyridoxal-5'-phosphate: K54 (= K56), N85 (= N87), I188 (= I190), G189 (= G191), T190 (= T192), G191 (= G193), G192 (= G194), T193 (= T195), G233 (= G235), S277 (= S279), P304 (= P306)

P47999 Cysteine synthase, chloroplastic/chromoplastic; At.OAS.7-4; Beta-substituted Ala synthase 2;1; ARAth-Bsas2;1; CSase B; AtCS-B; CS-B; O-acetylserine (thiol)-lyase; O-acetylserine sulfhydrylase; OAS-TL B; cpACS1; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
60% identity, 94% coverage: 19:321/322 of query aligns to 81:381/392 of P47999

query
sites
P47999

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A

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A

A

A

I

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V

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A

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K

R

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P

E

E

N

N

A

A

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G

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K

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A

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F

F

A

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Sites not aligning to the query:

61 modified: N-acetylalanine

5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
59% identity, 96% coverage: 13:321/322 of query aligns to 4:309/310 of 5xoqA

query
sites
5xoqA

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binding : T72 (= T84), S73 (= S85), G74 (= G86), T76 (= T88), M123 (= M135), Q144 (= Q157), R218 (≠ P230), H219 (= H231), Q222 (= Q234), G223 (= G235), A226 (= A238)

P47998 Cysteine synthase 1; At.OAS.5-8; Beta-substituted Ala synthase 1;1; ARAth-Bsas1;1; CSase A; AtCS-A; Cys-3A; O-acetylserine (thiol)-lyase 1; OAS-TL A; O-acetylserine sulfhydrylase; Protein ONSET OF LEAF DEATH 3; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
60% identity, 96% coverage: 12:319/322 of query aligns to 4:309/322 of P47998

query
sites
P47998

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K46 (= K56) modified: N6-(pyridoxal phosphate)lysine; mutation to A: No cysteine synthase activity.
T74 (= T84) mutation to A: Strong reduction of cysteine synthase activity.; mutation to S: Reduction of cysteine synthase activity.
S75 (= S85) mutation S->A,N,T: Strong reduction of cysteine synthase activity.
N77 (= N87) binding pyridoxal 5'-phosphate; mutation to A: Reduction of cysteine synthase activity.; mutation to D: Strong reduction of cysteine synthase activity.
T78 (= T88) mutation T->A,S: Reduction of cysteine synthase activity.
Q147 (= Q157) mutation Q->A,E: Strong reduction of cysteine synthase activity.
H157 (= H167) mutation H->Q,N: Slight reduction of cysteine synthase activity.
G162 (≠ A172) mutation to E: In old3-1; displays a early leaf death phenotype. Abolishes cysteine synthase activity.
GTGGT 181:185 (= GTGGT 191:195) binding pyridoxal 5'-phosphate
T182 (= T192) mutation T->A,S: Slight reduction of cysteine synthase activity.
T185 (= T195) mutation T->A,S: Strong reduction of cysteine synthase activity.
K217 (≠ N227) mutation to A: Impaired interaction with SAT1.
H221 (= H231) mutation to A: Impaired interaction with SAT1.
K222 (= K232) mutation to A: Impaired interaction with SAT1.
S269 (= S279) binding pyridoxal 5'-phosphate; mutation to A: Strong reduction of cysteine synthase activity.; mutation to T: Reduction of cysteine synthase activity.

2isqA Crystal structure of o-acetylserine sulfhydrylase from arabidopsis thaliana in complex with c-terminal peptide from arabidopsis serine acetyltransferase (see paper)
60% identity, 96% coverage: 12:319/322 of query aligns to 2:307/320 of 2isqA

query
sites
2isqA

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active site: K44 (= K56), S267 (= S279)
binding pyridoxal-5'-phosphate: K44 (= K56), N75 (= N87), G177 (= G189), G179 (= G191), T180 (= T192), G181 (= G193), T183 (= T195), G223 (= G235), S267 (= S279), P294 (= P306)
binding : T72 (= T84), S73 (= S85), G74 (= G86), T76 (= T88), G122 (= G134), M123 (= M135), K124 (= K136), G217 (= G229), P218 (= P230), H219 (= H231), Q222 (= Q234), G223 (= G235)

7n2tA O-acetylserine sulfhydrylase from citrullus vulgaris in the internal aldimine state, with citrate bound (see paper)
59% identity, 95% coverage: 17:321/322 of query aligns to 7:309/309 of 7n2tA

query
sites
7n2tA

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E

E

N

N

A

A

G

G

K

K

N

L

I

I

V

V

I

A

I

V

V

F

P

P

S

S

F

F

A

G

E

E

R

R

Y

Y

L

L

S

S

T

T

A

V

L

L

F

F

E

E

G

S

L

V

binding phosphate ion: R277 (≠ E289), R281 (= R293)

1z7yA Crystal structure of the arabidopsis thaliana o-acetylserine sulfhydrylase k46a mutant (see paper)
59% identity, 96% coverage: 12:319/322 of query aligns to 2:307/320 of 1z7yA

query
sites
1z7yA

R

R

I

I

Y

A

G

K

S

D

I

V

T

T

E

E

T

L

I

I

G

G

D

N

T

T

P

P

I

L

V

V

R

Y

L

L

D

N

K

N

L

V

A

A

K

E

E

-

K

-

G

G

V

C

K

V

A

G

N

R

L

V

L

A

A

A

K

K

L

L

E

E

F

M

N

E

P

P

I

C

A

S

S

S

V

V

K
x
A

D

D

R

R

I

I

G

G

V

F

S

S

L

M

I

I

D

S

A

D

L

A

E

E

K

K

Q

K

G

G

K

L

I

I

E

K

P

P

G

G

K

E

N

S

T

V

L

L

I

I

E

E

P

P

T

T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

F

F

V

T

A

A

R

K

G

G

Y

Y

K

K

L

L

I

I

L

I

V

T

M

M

P

P

E

A

T

S

M

M

S

S

I

T

E

E

R

R

K

I

M

I

L

L

A

L

L

A

L

F

G

G

A

V

E

E

L

L

V

V

L

L

T

T

P

D

G

P

A

A

Q

K

G

G

M

M

K

K

G

G

A

A

V

I

A

A

K

K

A

A

N

E

E

E

L

I

L

L

G

A

E

K

Y

T

P

P

N

N

A

G

V

Y

V

M

P

L

Q

Q

Q
|
Q

F

F

E

E

N

N

P

P

A

A

N

N

P

P

E

K

I

I

H

H

R

Y

R

E

T

T

A

G

E

P

E

E

I

I

W

W

N

K

D

G

T

T

E

G

H

G

K

K

V

I

D

D

I

G

V

F

V

V

S

S

G

G

I
|
I

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

A

G

G

Q

K

V

Y

L

L

K

K

P

E

R

Q

K

N

P

A

S

N

L

V

K

K

I

L

I

Y

A

G

V

V

E

E

P

P

E

V

E

E

S

S

P

A

V

I

L

L

S

S

G

G

N

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

G

S

V

V

L

L

D

N

R

V

D

D

V

L

I

I

D

D

E

E

I

V

V

V

K

Q

V

V

N

S

S

S

A

D

T

E

A

S

L

I

E

D

T

M

A

A

R

R

H

Q

V

L

A

A

R

L

L

K

E

E

G

G

I

L

P

L

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

I

A

A

A

I

I

E

K

V

L

G

A

S

Q

R

R

P

P

E

E

N

N

A

A

G

G

K

K

N

L

I

F

V

V

I

A

I

I

V

F

P
|
P

S
|
S

F

F

A

G

E

E

R

R

Y

Y

L

L

S

S

T

T

A

V

L

L

F

F

E

D

active site: A44 (≠ K56), S267 (= S279)
binding n-[(3-hydroxy-2-methyl-5-{[(trihydroxyphosphoranyl)oxy]methyl}pyridin-4-yl)methylene]methionine: G74 (= G86), N75 (= N87), T76 (= T88), Q145 (= Q157), I178 (= I190), G179 (= G191), T180 (= T192), G181 (= G193), T183 (= T195), G223 (= G235), S267 (= S279), P294 (= P306), S295 (= S307)

8b9wA Cysteine synthase from trypanosoma theileri with plp bound (see paper)
54% identity, 94% coverage: 16:318/322 of query aligns to 12:311/329 of 8b9wA

query
sites
8b9wA

S

S

I

V

T

D

E

A

T

L

I

I

G

G

D

N

T

T

P

P

I

A

V

V

R

Y

L

L

D

R

K

N

L

M

A

N

K

K

E

-

K

-

G

-

V

T

K

A

A

A

N

T

L

I

L

V

A

L

K

K

L

L

E

E

F

S

F

E

N

N

P

P

I

M

A

A

S

S

V

V

K
|
K

D

D

R

R

I

L

G

A

V

Y

S

A

L

I

I

Y

D

D

A

K

L

A

E

E

K

K

Q

E

G

G

K

K

I

I

E

K

P

R

G

G

K

K

N

S

T

V

L

I

I

V

E

E

P

A

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

F

L

V

L

A

G

A

A

A

V

R

R

G

G

Y

Y

K

K

L

V

I

I

L

I

V

T

M

M

P

P

E

D

T

S

M

M

S

S

I

M

E

E

R

R

K

S

M

L

L

L

A

R

L

I

L

F

G

G

A

A

E

E

L

L

V

I

L

L

T

T

P

P

G

A

A

S

Q

L

G

G

M

M

K

K

G

G

A

A

V

V

A

A

K

M

A

A

N

N

E

R

L

I

L

V

G

S

E

N

Y

N

P

P

N

D

A

A

V

V

V

L

P

A

Q

D

Q

Q

F

F

E

C

N

T

P

K

A

Y

N

N

P

A

E

Q

I

I

H

H

R

E

T

T

A

G

E

P

E

E

I

I

W

W

N

R

D

Q

T

T

E

K

H

G

K

H

V

V

D

D

I

C

V

F

V

V

S

A

G

G

I

V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

L

T

T

G

G

V

V

G

A

Q

R

V

F

L

L

K

K

P

S

R

V

K

G

P

C

S

N

L

A

K

N

I

I

I

V

A

A

V

V

E

E

P

P

E

Q

E

E

S

S

P

P

V

V

L

L

S

S

G

G

N

R

P

P

G

G

P

P

H

H

K

R

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

V

P

P

G

E

V

V

L

L

D

D

R

R

D

E

V

L

I

V

D

D

E

E

I

I

V

I

K

Q

V

V

N

S

S

S

A

D

T

K

A

A

L

I

E

E

T

T

A

A

R

Q

H

K

V

L

A

S

R

R

L

M

E

D

G

G

I

I

P

F

V

C

G

G

I

F

S
|
S

S

A

G

G

A

A

A

N

I

V

A

F

A

A

I

L

E

K

V

I

G

A

S

E

R

R

P

P

E

E

N

M

A

A

G

G

K

K

N

T

I

I

V

V

I

T

I

V

V

I

P
|
P

S

S

F

F

A

G

E

E

R

R

Y

Y

L

L

S

S

T

T

A

A

L

L

F

Y

binding pyridoxal-5'-phosphate: K49 (= K56), N80 (= N87), G184 (= G191), T185 (= T192), G186 (= G193), T188 (= T195), G228 (= G235), S272 (= S279), P299 (= P306)

3t4pA Crystal structure of o-acetyl serine sulfhydrylase from leishmania donovani in complex with designed tetrapeptide (see paper)
53% identity, 95% coverage: 16:321/322 of query aligns to 13:315/319 of 3t4pA

query
sites
3t4pA

S

S

I

I

T

D

E

Q

T

L

I

I

G

G

D

Q

T

T

P

P

I

A

V

L

R

Y

L

L

D

N

K

K

L

L

A

-

K

-

E

-

K

N

G

N

V

T

K

K

A

A

N

K

L

V

A

L

K

K

L

M

E

E

F

C

F

E

N

N

P

P

I

M

A

A

S

S

V

V

K
|
K

D

D

R

R

I

L

G

G

V

F

S

A

L

I

I

Y

D

D

A

K

L

A

E

E

K

K

Q

E

G

G

K

K

I

L

E

I

P

P

G

G

K

K

N

S

T

I

L

V

I

V

E

E

P

S

T
x
S

S
|
S

G
|
G

N

N

T
|
T

G

G

I

V

A

S

L

L

A

A

F

H

V

L

A

G

A

A

A

I

R

R

G

G

Y

Y

K

K

L

V

I

I

L

I

V

T

M

M

P

P

E

E

T

S

M

M

S

S

I

L

E

E

R

R

K

C

M

L

L

L

A

R

L

I

L

F

G

G

A

A

E

E

L

V

V

I

L

L

T

T

P

P

G

A

A

A

Q

L

G

G

M
|
M

K

K

G

G

A

A

V

V

A

A

K

M

A

A

N

K

E

K

L

I

L

V

G

A

E

A

Y

N

P

P

N

N

A

A

V

V

V

L

P

A

Q

D

Q
|
Q

F
|
F

E

A

N

T

P

K

A

Y

N

N

P

A

E

L

I

I

H

H

R

E

T

T

A

G

E

P

E

E

I

I

W

W

N

E

D

Q

T

T

E

N

H

H

K

N

V

V

D

D

I

C

V

F

V

I

S

A

G

G

I

V

G

G

T

T

G

G

T

T

I

L

T

T

G

G

V

V

G

A

Q

R

V

A

L

L

K

K

P

K

R

M

K

G

P

S

S

H

L

A

K

R

I

I

I

V

A

A

V

V

E

E

P

P

E

T

E

E

S

S

P

P

V

V

L

L

S

S

G

G

N

K

P

P

G
|
G

P
|
P

H
|
H

K

K

I

I

Q

Q

G
|
G

I

I

G
|
G

A
x
P

G

G

F

F

V

V

P

P

G

D

V

V

L

L

D

D

R

R

D

S

V

L

I

I

D

D

E

E

I

V

V

L

K

C

V

V

N

A

S

G

A

D

T

D

A

A

L

I

E

E

T

T

A

A

R

L

H

K

V

L

A

T

R

R

L

S

E

D

G

G

I

V

P

F

V

C

G

G

I

F

S
|
S

S

G

G

G

A

A

A

N

I

V

A

Y

A

A

I

L

E

K

V

I

G

A

S

E

R

R

P

P

E

E

N

M

A

E

G

G

K

K

N

T

I

I

V

V

I

T

I

V

V

I

P

P

S

S

F

F

A

G

E

E

R

R

Y

Y

L

L

S

S

T

T

A

T

L

L

F

Y

E

R

G

S

L

V

active site: K50 (= K56), S273 (= S279)
binding : S78 (≠ T84), S79 (= S85), G80 (= G86), T82 (= T88), M129 (= M135), Q151 (= Q157), F152 (= F158), G223 (= G229), P224 (= P230), H225 (= H231), G229 (= G235), G231 (= G237), P232 (≠ A238)

P0A1E3 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; EC 2.5.1.47 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
55% identity, 96% coverage: 12:321/322 of query aligns to 3:315/323 of P0A1E3

query
sites
P0A1E3

R

K

I

I

Y

Y

G

E

S

D

I

N

T

S

E

L

T

T

I

I

G

G

D

H

T

T

P

P

I

L

V

V

R

R

L

L

D

N

K

R

L

I

A

G

K

N

E

-

K

-

G

-

V

-

K

-

A

G

N

R

L

I

L

L

A

A

K

K

L

V

E

E

F

S

F

R

N

N

P

P

I

S

A

F

S

S

V

V

K

K

D

C

R

R

I

I

G

G

V

A

S

N

L

M

I

I

D

W

A

D

L

A

E

E

K

K

Q

R

G

G

K

V

I

L

E

K

P

P

G

G

K

V

N

E

T

-

L

L

I

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

F

Y

V

V

A

A

R

R

G

G

Y

Y

K

K

L

L

I

T

L

L

V

T

M

M

P

P

E

E

T

T

M

M

S

S

I

I

E

E

R

R

K

K

M

L

L

L

A

K

L

A

L

L

G

G

A

A

E

N

L

L

V

V

L

L

T

T

P

E

G

G

A

A

Q

K

G

G

M

M

K

K

G

G

A

A

V

I

A

Q

K

K

A

A

N

E

E

E

L

I

L

V

G

A

E

S

Y

D

P

P

N

Q

A

K

-

Y

V

L

P

L

Q

Q

F

F

E

S

N

N

P

P

A

A

N

N

P

P

E

E

I

I

H

H

R

E

R

K

T

T

A

G

E

P

E

E

I

I

W

W

N

E

D

D

T

T

E

D

H

G

K

Q

V

V

D

D

I

V

V

F

V

I

S

S

G

G

I

V

G

G

T

T

G

G

T

T

I

L

T

T

G

G

V

V

G

T

Q

R

V

Y

L

I

K

K

P

G

R

T

K

K

-

G

-

K

P

T

S

D

L

L

K

I

I

T

I

V

A

A

V

V

E

E

P

P

E

T

E

D

S

S

P

P

V

V

L

I

S

A

G

Q

G

A

-

L

-

A

-

G

-

E

-

I

N

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

G

G

V

N

L

L

D

D

R

L

D

K

V

L

I

I

D

D

E

K

I

V

V

V

K

G

V

I

N

T

S

N

A

E

T

E

A

A

L

I

E

S

T

T

A

A

R

R

H

R

V

L

A

M

R

E

L

E

E

E

G

G

I

I

P

L

V

A

G

G

I

I

S
|
S

S

S

G

G

A

A

I

V

A

A

I

L

E

K

V

L

G

Q

S

E

R

D

P

E

E

S

N

F

A

T

G

N

K

K

N

N

I

I

V

V

I

V

I

I

V

L

P

P

S

S

F

S

A

G

E

E

R

R

Y

Y

L

L

S

S

T

T

A

A

L

L

F

F

E

A

G

D

L

L

N72 (= N87) binding pyridoxal 5'-phosphate
S273 (= S279) binding pyridoxal 5'-phosphate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

6z4nAAA structure of oass complexed with upar inhibitor (see paper)
55% identity, 96% coverage: 12:321/322 of query aligns to 4:316/321 of 6z4nAAA

query
sites
6z4nAAA

R

K

I

I

Y

Y

G

E

S

D

I

N

T

S

E

L

T

T

I

I

G

G

D

H

T

T

P

P

I

L

V

V

R

R

L

L

D

N

K

R

L

I

A

G

K

N

E

-

K

-

G

-

V

-

K

-

A

G

N

R

L

I

L

L

A

A

K

K

L

V

E

E

F

S

F

R

N

N

P

P

I

S

A

F

S

S

V

V

K
|
K

D

C

R

R

I

I

G

G

V

A

S

N

L

M

I

I

D

W

A

D

L

A

E

E

K

K

Q

R

G

G

K

V

I

L

E

K

P

P

G

G

K

V

N

E

T

-

L

L

I

V

E

E

P

P

T
|
T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

F

Y

V

V

A

A

R

R

G

G

Y

Y

K

K

L

L

I

T

L

L

V

T

M

M

P

P

E

E

T

T

M

M

S

S

I

I

E

E

R

R

K

K

M

L

L

L

A

K

L

A

L

L

G

G

A

A

E

N

L

L

V

V

L

L

T

T

P

E

G

G

A

A

Q

K

G

G

M

M

K

K

G

G

A

A

V

I

A

Q

K

K

A

A

N

E

E

E

L

I

L

V

G

A

E

S

Y

D

P

P

N

Q

A

K

-

Y

V

L

P

L

Q

Q

Q
|
Q

F
|
F

E

S

N

N

P

P

A

A

N

N

P

P

E

E

I

I

H

H

R

E

R

K

T

T

A

G

E

P

E

E

I

I

W

W

N

E

D

D

T

T

E

D

H

G

K

Q

V

V

D

D

I

V

V

F

V

I

S

S

G

G

I
x
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

L

T

T

G

G

V

V

G

T

Q

R

V

Y

L

I

K

K

P

G

R

T

K

K

-

G

-

K

P

T

S

D

L

L

K

I

I

T

I

V

A

A

V

V

E

E

P

P

E

T

E

D

S

S

P

P

V

V

L

I

S

A

G

Q

G

A

-

L

-

A

-

G

-

E

-

I

N

K

P

P

G

G

P

P

H

H

K

K

I

I

Q
|
Q

G
|
G

I
|
I

G

G

A
|
A

G

G

F

F

V

I

P

P

G

G

V

N

L

L

D

D

R

L

D

K

V

L

I

I

D

D

E

K

I

V

V

V

K

G

V

I

N

T

S

N

A

E

T

E

A

A

L

I

E

S

T

T

A

A

R

R

H

R

V

L

A

M

R

E

L

E

E

E

G

G

I

I

P

L

V

A

G

G

I

I

S
|
S

S

S

G

G

A

A

I

V

A

A

I

L

E

K

V

L

G

Q

S

E

R

D

P

E

E

S

N

F

A

T

G

N

K

K

N

N

I

I

V

V

I

V

I

I

V

L

P
|
P

S

S

F

S

A

G

E

E

R

R

Y

Y

L

L

S

S

T

T

A

A

L

L

F

F

E

A

G

D

L

L

binding pyridoxal-5'-phosphate: K43 (= K56), N73 (= N87), V177 (≠ I190), G178 (= G191), T179 (= T192), G180 (= G193), T182 (= T195), G230 (= G235), S274 (= S279), P301 (= P306)
binding (1~{S},2~{S})-1-[(4-methylphenyl)methyl]-2-phenyl-cyclopropane-1-carboxylic acid: K43 (= K56), T70 (= T84), G72 (= G86), N73 (= N87), T74 (= T88), Q144 (= Q157), F145 (= F158), Q229 (= Q234), G230 (= G235), I231 (= I236), A233 (= A238)

8b9yC Cysteine synthase from trypanosoma cruzi with plp and oas (see paper)
51% identity, 97% coverage: 10:321/322 of query aligns to 7:315/330 of 8b9yC

query
sites
8b9yC

R

R

G

N

R

N

I

V

Y

A

G

P

S

S

I

M

T

D

E

A

T

L

I

I

G

G

D

E

T

T

P

P

I

A

V

V

R

Y

L

L

D

K

K

R

L

M

A

-

K

-

E

-

K

N

G

D

V

T

K

A

A

A

N

T

L

I

L

V

A

L

K

K

L

L

E

E

F

C

F

E

N

N

P

P

I

M

A

A

S

S

V

V

K
|
K

D

D

R

R

I

L

G

A

V

Y

S

A

L

I

I

Y

D

D

A

K

L

A

E

E

K

K

Q

E

G

G

K

K

I

I

E

I

P

P

G

G

K

K

N

S

T

V

L

I

I

V

E

E

P

A

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

F

H

V

I

A

G

A

T

A

I

R

R

G

G

Y

Y

K

K

L

V

I

I

L

I

V

V

M

M

P

P

E

E

T

S

M

M

S

S

I

I

E

E

R

R

K

C

M

L

L

M

A

R

L

I

L

F

G

G

A

A

E

E

L

V

V

I

L

L

T

T

P

P

G

A

A

A

Q

L

G

G

M

M

K

K

G

G

A

A

V

L

A

E

K

A

A

V

N

N

E

R

L

I

L

V

G

S

E

N

Y

N

P

P

N

D

A

A

V

V

V

S

P

A

Q

N

Q

Q

F

F

E

A

N

T

P

K

A

Y

N

N

P

A

E

Q

I

I

H

H

R

E

T

T

A

G

E

P

E

E

I

I

W

W

N

R

D

Q

T

T

E

K

H

G

K

H

V

V

D

D

I

C

V

F

V

V

S

A

G

G

I

V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

V

G

A

Q

R

V

Y

L

L

K

K

P

S

R

V

K

G

P

C

S

G

L

A

K

T

I

I

I

F

A

A

V

V

E

E

P

P

E

A

E

E

S

S

P

P

V

V

L

L

S

S

G

G

N

K

P

P

G

G

P

P

H

H

K

R

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

V

P

P

G

E

V

V

L

F

D

E

R

A

D

A

V

L

I

V

D

D

E

E

I

V

V

I

K

Q

V

V

N

S

S

G

A

D

T

E

A

A

L

I

E

D

T

T

A

A

R

Q

H

K

V

L

A

P

R

R

L

T

E

D

G

G

I

I

P

F

V

C

G

G

I

F

S
|
S

S

G

G

G

A

A

A

N

I

V

A

Y

A

A

I

L

E

Q

V

I

G

A

S

K

R

R

P

P

E

E

N

M

A

A

G

G

K

K

N

T

I

I

V

V

I

T

I

V

V

I

P
|
P

S
|
S

F

Y

A

G

E

E

R

R

Y

Y

L

L

S

S

T

T

A

A

L

L

F

Y

E

S

G

S

L

I

binding pyridoxal-5'-phosphate: K50 (= K56), N81 (= N87), G185 (= G191), T186 (= T192), G187 (= G193), T189 (= T195), G229 (= G235), S273 (= S279), P300 (= P306), S301 (= S307)

1d6sA Crystal structure of the k41a mutant of o-acetylserine sulfhydrylase complexed in external aldimine linkage with methionine (see paper)
54% identity, 96% coverage: 12:321/322 of query aligns to 2:314/322 of 1d6sA

query
sites
1d6sA

R

K

I

I

Y

Y

G

E

S

D

I

N

T

S

E

L

T

T

I

I

G

G

D

H

T

T

P

P

I

L

V

V

R

R

L

L

D

N

K

R

L

I

A

G

K

N

E

-

K

-

G

-

V

-

K

-

A

G

N

R

L

I

L

L

A

A

K

K

L

V

E

E

F

S

F

R

N

N

P

P

I

S

A

F

S

S

V

V

K
x
A

D

C

R

R

I

I

G

G

V

A

S

N

L

M

I

I

D

W

A

D

L

A

E

E

K

K

Q

R

G

G

K

V

I

L

E

K

P

P

G

G

K

V

N

E

T

-

L

L

I

V

E

E

P

P

T
|
T

S
x
N

G

G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

F

Y

V

V

A

A

R

R

G

G

Y

Y

K

K

L

L

I

T

L

L

V

T

M

M

P

P

E

E

T

T

M

M

S

S

I

I

E

E

R

R

K

K

M

L

L

L

A

K

L

A

L

L

G

G

A

A

E

N

L

L

V

V

L

L

T

T

P

E

G

G

A

A

Q

K

G

G

M

M

K

K

G

G

A

A

V

I

A

Q

K

K

A

A

N

E

E

E

L

I

L

V

G

A

E

S

Y

D

P

P

N

Q

A

K

-

Y

V

L

P

L

Q

Q

Q
|
Q

F
|
F

E

S

N

N

P

P

A

A

N

N

P

P

E

E

I

I

H

H

R

E

R

K

T

T

A

G

E

P

E

E

I

I

W

W

N

E

D

D

T

T

E

D

H

G

K

Q

V

V

D

D

I

V

V

F

V

I

S

S

G

G

I

V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

L

T

T

G

G

V

V

G

T

Q

R

V

Y

L

I

K

K

P

G

R

T

K

K

-

G

-

K

P

T

S

D

L

L

K

I

I

T

I

V

A

A

V

V

E

E

P

P

E

T

E

D

S

S

P

P

V

V

L

I

S

A

G

Q

G

A

-

L

-

A

-

G

-

E

-

I

N

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

G

G

V

N

L

L

D

D

R

L

D

K

V

L

I

I

D

D

E

K

I

V

V

V

K

G

V

I

N

T

S

N

A

E

T

E

A

A

L

I

E

S

T

T

A

A

R

R

H

R

V

L

A

M

R

E

L

E

E

E

G

G

I

I

P

L

V

A

G

G

I

I

S
|
S

S

S

G

G

A

A

I

V

A

A

I

L

E

K

V

L

G

Q

S

E

R

D

P

E

E

S

N

F

A

T

G

N

K

K

N

N

I

I

V

V

I

V

I

I

V

L

P
|
P

S

S

F

S

A

G

E

E

R

R

Y

Y

L

L

S

S

T

T

A

A

L

L

F

F

E

A

G

D

L

L

active site: A41 (≠ K56), G228 (= G235)
binding methionine: T68 (= T84), N69 (≠ S85), N71 (= N87), T72 (= T88), Q142 (= Q157), F143 (= F158), G176 (= G191), G228 (= G235)
binding pyridoxal-5'-phosphate: N71 (= N87), G176 (= G191), T177 (= T192), G178 (= G193), T180 (= T195), G228 (= G235), S272 (= S279), P299 (= P306)

3vbeC Crystal structure of beta-cyanoalanine synthase in soybean (see paper)
52% identity, 95% coverage: 17:321/322 of query aligns to 15:317/329 of 3vbeC

query
sites
3vbeC

I

V

T

S

E

Q

T

L

I

I

G

G

D

R

T

T

P

P

I

L

V

V

R

Y

L

L

D

N

K

K

L

V

A

T

K

E

E

-

K

-

G

G

V

C

K

G

A

A

N

Y

L

V

L

A

A

V

K

K

L

Q

E

E

F

M

N

Q

P

P

I

T

A

A

S

S

V

I

K
|
K

D

D

R

R

I

P

G

A

V

Y

S

A

L

M

I

I

D

T

A

D

L

A

E

E

K

E

Q

K

G

N

K

L

I

I

E

T

P

P

G

G

K

K

N

T

T

T

L

L

I

I

E

E

P

P

T

T

S
|
S

G

G

N
|
N

T

M

G

G

I

I

A

S

L

M

A

A

F

F

V

M

A

A

A

M

R

K

G

G

Y

Y

K

K

L

M

I

V

L

L

V

T

M

M

P

P

E

S

T

Y

M

T

S

S

I

L

E

E

R

R

K

V

M

T

L

M

A

R

L

A

L

F

G

G

A

A

E

E

L

L

V

I

L

L

T

T

P

D

G

P

A

A

Q

K

G

G

M

M

K

G

G

G

A

T

V

V

A

K

K

K

A

A

N

Y

E

E

L

L

G

E

E

N

Y

T

P

P

N

N

A

A

V

H

V

M

P

L

Q

Q

F

F

E

S

N

N

P

P

A

A

N

N

P

T

E

Q

I

V

H

H

R

F

R

E

T

T

A

G

E

P

E

E

I

I

W

W

N

E

D

D

T

T

E

N

H

G

K

Q

V

V

D

D

I

I

V

F

V

V

S
x
M

G

G

I

I

G
|
G

T
x
S

G
|
G

G

G

T
|
T

I

V

T

S

G

G

V

V

G

G

Q

Q

V

Y

L

L

K

K

P

S

R

K

K

N

P

P

S

N

L

V

K

K

I

I

I

Y

A

G

V

V

E
|
E

P

P

E

S

E

E

S
|
S

P

N

V

V

L

L

S

N

G

G

N

K

P

P

G

G

P

P

H

H

K

H

I

I

Q

T

G
|
G

I

N

G

G

A

V

G

G

F

F

V

K

P

P

G

D

V

I

L

L

D

D

R

L

D

D

V

V

I

M

D

E

E

K

I

V

V

L

K

E

V

V

N

S

S

S

A

E

T

D

A

A

L

V

E

N

T

M

A

A

R

R

H

V

V

L

A

A

R

L

L

K

E

E

G

G

I

L

P

M

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

A

N

I

T

A

V

A

A

I

L

E

R

V

L

G

A

S

Q

R

L

P

P

E

E

N

N

A

K

G

G

K

K

N

L

I

I

V

V

I

T

I

V

V

H

P
|
P

S

S

F

F

A

G

E

E

R

R

Y

Y

L

L

S

S

T

S

A

V

L

L

F

F

E

Q

G

E

L

L

active site: K52 (= K56), S81 (= S85), E212 (= E216), S216 (= S220), S275 (= S279), P302 (= P306)
binding pyridoxal-5'-phosphate: K52 (= K56), N83 (= N87), M184 (≠ S188), G187 (= G191), S188 (≠ T192), G189 (= G193), T191 (= T195), G231 (= G235), S275 (= S279), P302 (= P306)

Query Sequence

>WP_013419965.1 NCBI__GCF_000166055.1:WP_013419965.1
MAEKNHAPGRGRIYGSITETIGDTPIVRLDKLAKEKGVKANLLAKLEFFNPIASVKDRIG
VSLIDALEKQGKIEPGKNTLIEPTSGNTGIALAFVAAARGYKLILVMPETMSIERRKMLA
LLGAELVLTPGAQGMKGAVAKANELLGEYPNAVVPQQFENPANPEIHRRTTAEEIWNDTE
HKVDIVVSGIGTGGTITGVGQVLKPRKPSLKIIAVEPEESPVLSGGNPGPHKIQGIGAGF
VPGVLDRDVIDEIVKVNSATALETARHVARLEGIPVGISSGAAIAAAIEVGSRPENAGKN
IVIIVPSFAERYLSTALFEGLA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory