SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013420262.1 NCBI__GCF_000166055.1:WP_013420262.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

7bgmA Crystal structure of mthisn2, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
39% identity, 79% coverage: 5:92/111 of query aligns to 127:212/213 of 7bgmA

query
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7bgmA

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binding zinc ion: E163 (= E44), E183 (= E63), D186 (= D66)

Sites not aligning to the query:

binding zinc ion: 80, 82, 84, 97, 104

7bgnA Crystal structure of mthisn2-amp complex, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
39% identity, 79% coverage: 5:92/111 of query aligns to 117:203/204 of 7bgnA

query
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7bgnA

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binding adenosine monophosphate: L121 (= L9), R128 (= R16), S135 (= S24), W136 (≠ Y25), T137 (= T26), Y180 (= Y69), R203 (= R92)
binding zinc ion: E154 (= E44), E174 (= E63), D177 (= D66)

Sites not aligning to the query:

binding adenosine monophosphate: 60, 62, 63, 65, 66, 94, 96, 204
binding zinc ion: 78, 80, 82, 95, 102

6j2lA Crystal structure of bi-functional enzyme (see paper)
37% identity, 59% coverage: 6:70/111 of query aligns to 114:178/200 of 6j2lA

query
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6j2lA

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binding zinc ion: E152 (= E44), E171 (= E63), D174 (= D66)

Sites not aligning to the query:

binding zinc ion: 79, 81, 83, 96, 103

Query Sequence

>WP_013420262.1 NCBI__GCF_000166055.1:WP_013420262.1
MSEHTLARLAATIKARRASTASASYTKSLLEKGAAACAKKLGEEAVETVIAGVAESDDKL
RAEAADLLYHLMVLLEARNVPIEAVFAELDSRTAQSGHAEKASRPKDGSPS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory