SitesBLAST
Comparing WP_013420474.1 NCBI__GCF_000166055.1:WP_013420474.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
6iunB Crystal structure of enoyl-coa hydratase (ech) from ralstonia eutropha h16 in complex with NAD
46% identity, 98% coverage: 8:685/695 of query aligns to 4:683/692 of 6iunB
- active site: A60 (= A64), F65 (≠ L69), E73 (≠ D77), H77 (≠ N79), G101 (≠ R103), E104 (= E106), E124 (= E126), G132 (= G134), K248 (= K252), S407 (= S411), H428 (= H432), E440 (= E444), N478 (= N482)
- binding nicotinamide-adenine-dinucleotide: G300 (= G304), T301 (≠ H305), M302 (= M306), E321 (≠ D325), T322 (≠ R326), Y365 (= Y369), A377 (= A381), V378 (≠ A382), E380 (= E384), V384 (= V388), V388 (= V392), N405 (= N409), S407 (= S411)
Q08426 Peroxisomal bifunctional enzyme; PBE; PBFE; L-bifunctional protein; LBP; Multifunctional enzyme 1; MFE1; EC 4.2.1.17; EC 5.3.3.8; EC 1.1.1.35 from Homo sapiens (Human) (see 5 papers)
41% identity, 98% coverage: 12:691/695 of query aligns to 9:707/723 of Q08426
- V40 (≠ E43) to G: in dbSNP:rs1062551
- I41 (≠ A44) to R: in dbSNP:rs1062552
- T75 (≠ D77) to I: in dbSNP:rs1062553
- K165 (≠ T167) modified: N6-acetyllysine; alternate; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-171; Q-346 and Q-584.
- K171 (≠ E173) modified: N6-acetyllysine; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-346 and Q-584.
- A274 (= A276) to T: in dbSNP:rs2302819
- A325 (≠ V323) to G: in dbSNP:rs1062555
- K346 (≠ S344) modified: N6-acetyllysine; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-171 and Q-584.
- K584 (≠ T571) modified: N6-acetyllysine; alternate; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-171 and Q-346.
- K598 (≠ A584) to T: in dbSNP:rs1042437
- T606 (≠ E592) to P: in dbSNP:rs1042438
Sites not aligning to the query:
- 3 E → K: in FRTS3; the mutant is mistargeted to mitochondria; results in impaired mitochondrial oxidative phosphorylation and defects in the transport of fluids across the epithelium of renal proximal tubular cells; dbSNP:rs398124646
3zw9A Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with (2s,3s)-3-hydroxy-2-methylbutanoyl-coa (see paper)
40% identity, 98% coverage: 14:691/695 of query aligns to 14:709/723 of 3zw9A
- active site: A64 (= A64), F69 (≠ L69), G79 (≠ N79), G103 (≠ R103), E106 (= E106), P125 (= P125), E126 (= E126), P133 (= P133), G134 (= G134), K252 (= K252), S413 (= S411), H434 (= H432), E446 (= E444), N484 (= N482)
- binding nicotinamide-adenine-dinucleotide: L305 (≠ V301), G306 (= G302), G308 (= G304), T309 (≠ H305), M310 (= M306), E329 (≠ D325), Q334 (≠ A330), A383 (= A381), V384 (≠ A382), F385 (= F383), E386 (= E384), N411 (= N409), S413 (= S411), H434 (= H432)
- binding (2s,3s)-3-hydroxy-2-methylbutanoyl-coa: V24 (≠ L24), A62 (= A62), G63 (= G63), A64 (= A64), I66 (= I66), G102 (≠ A102), G103 (≠ R103), E106 (= E106), E126 (= E126), P133 (= P133), Y159 (≠ L159)
3zwaA Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with 3s-hydroxy-hexanoyl-coa (see paper)
40% identity, 98% coverage: 14:691/695 of query aligns to 17:712/727 of 3zwaA
- active site: A67 (= A64), F72 (≠ L69), G82 (≠ N79), G106 (≠ R103), E109 (= E106), P128 (= P125), E129 (= E126), P136 (= P133), G137 (= G134), K255 (= K252), S416 (= S411), H437 (= H432), E449 (= E444), N487 (= N482)
- binding (S)-3-Hydroxyhexanoyl-CoA: V27 (≠ L24), A65 (= A62), G66 (= G63), A67 (= A64), D68 (= D65), I69 (= I66), L104 (≠ V101), E109 (= E106), R124 (= R121), E129 (= E126), L132 (≠ V129), G137 (= G134), Y162 (≠ L159)
5omoA Crystal structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with with 3s-hydroxy-decanoyl-coa and 3-keto- decanoyl-coa
40% identity, 98% coverage: 14:691/695 of query aligns to 16:711/725 of 5omoA
- active site: A66 (= A64), F71 (≠ L69), G81 (≠ N79), G105 (≠ R103), E108 (= E106), P127 (= P125), E128 (= E126), P135 (= P133), G136 (= G134), K254 (= K252), S415 (= S411), H436 (= H432), E448 (= E444), N486 (= N482)
- binding (s)-3-hydroxydecanoyl-coa: P25 (= P23), V26 (≠ L24), A28 (≠ V26), P31 (vs. gap), A64 (= A62), A66 (= A64), D67 (= D65), I68 (= I66), L103 (≠ V101), G105 (≠ R103), E108 (= E106), P127 (= P125), E128 (= E126), Y161 (≠ L159), F260 (= F258), K280 (≠ R278)
- binding 3-keto-decanoyl-coa: S415 (= S411), N486 (= N482), K519 (≠ A515), M520 (= M516), V525 (= V521), Y658 (= Y640)
5mgbA Crystal structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with acetoacetyl-coa and NAD (see paper)
40% identity, 98% coverage: 14:691/695 of query aligns to 16:711/725 of 5mgbA
- active site: A66 (= A64), F71 (≠ L69), G81 (≠ N79), G105 (≠ R103), E108 (= E106), P127 (= P125), E128 (= E126), P135 (= P133), G136 (= G134), K254 (= K252), S415 (= S411), H436 (= H432), E448 (= E444), N486 (= N482)
- binding acetoacetyl-coenzyme a: P25 (= P23), V26 (≠ L24), A64 (= A62), G65 (= G63), A66 (= A64), D67 (= D65), I68 (= I66), G105 (≠ R103), E128 (= E126), Y161 (≠ L159)
- binding nicotinamide-adenine-dinucleotide: L307 (≠ V301), G308 (= G302), G310 (= G304), T311 (≠ H305), M312 (= M306), E331 (≠ D325), S332 (≠ R326), Q336 (≠ A330), V386 (≠ A382), F387 (= F383), E388 (= E384), N413 (= N409), S415 (= S411), H436 (= H432)
3zwcA Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with 3s-hydroxy-decanoyl-coa (see paper)
40% identity, 98% coverage: 14:691/695 of query aligns to 16:711/725 of 3zwcA
- active site: A66 (= A64), F71 (≠ L69), G81 (≠ N79), G105 (≠ R103), E108 (= E106), P127 (= P125), E128 (= E126), P135 (= P133), G136 (= G134), K254 (= K252), S415 (= S411), H436 (= H432), E448 (= E444), N486 (= N482)
- binding (s)-3-hydroxydecanoyl-coa: V26 (≠ L24), A64 (= A62), G65 (= G63), A66 (= A64), D67 (= D65), I68 (= I66), G77 (= G75), L78 (≠ A76), L80 (≠ I78), V101 (≠ T99), G104 (≠ A102), G105 (≠ R103), E108 (= E106), E128 (= E126), F260 (= F258)
- binding nicotinamide-adenine-dinucleotide: G308 (= G302), G310 (= G304), T311 (≠ H305), M312 (= M306), E331 (≠ D325), Q336 (≠ A330), A385 (= A381), V386 (≠ A382), F387 (= F383), E388 (= E384), K393 (= K389), N413 (= N409), S415 (= S411), H436 (= H432)
2x58A The crystal structure of mfe1 liganded with coa (see paper)
40% identity, 98% coverage: 14:691/695 of query aligns to 16:711/725 of 2x58A
- active site: A66 (= A64), F71 (≠ L69), G81 (≠ N79), G105 (≠ R103), E108 (= E106), P127 (= P125), E128 (= E126), P135 (= P133), G136 (= G134), K254 (= K252), S415 (= S411), H436 (= H432), E448 (= E444), N486 (= N482)
- binding adenosine-5'-diphosphate: G310 (= G304), T311 (≠ H305), M312 (= M306), E331 (≠ D325), S332 (≠ R326), Q336 (≠ A330), V386 (≠ A382), L392 (≠ V388)
- binding coenzyme a: V26 (≠ L24), A28 (≠ V26), A64 (= A62), A66 (= A64), D67 (= D65), I68 (= I66), E128 (= E126)
6zibAAA structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with acetoacetyl-coa and nadh'
41% identity, 98% coverage: 14:691/695 of query aligns to 16:709/723 of 6zibAAA
- active site: A66 (= A64), F71 (≠ L69), G81 (≠ N79), G105 (≠ R103), E108 (= E106), P127 (= P125), E128 (= E126), G136 (= G134), K254 (= K252), S413 (= S411), H434 (= H432), E446 (= E444), N484 (= N482)
- binding acetoacetyl-coenzyme a: P25 (= P23), V26 (≠ L24), A64 (= A62), G65 (= G63), A66 (= A64), D67 (= D65), I68 (= I66), G104 (≠ A102), G105 (≠ R103), E128 (= E126), Y161 (≠ L159)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G310 (= G304), T311 (≠ H305), M312 (= M306), E331 (≠ D325), S332 (≠ R326), Q336 (≠ A330), A383 (= A381), V384 (≠ A382), F385 (= F383), E386 (= E384), N411 (= N409), H434 (= H432)
6zicAAA structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with 3s-hydroxybutanoyl-coa and nadh'
40% identity, 98% coverage: 14:691/695 of query aligns to 16:709/723 of 6zicAAA
- active site: A66 (= A64), F71 (≠ L69), G81 (≠ N79), G105 (≠ R103), E108 (= E106), P127 (= P125), E128 (= E126), G136 (= G134), K254 (= K252), S413 (= S411), H434 (= H432), E446 (= E444), N484 (= N482)
- binding 3-hydroxybutanoyl-coenzyme a: P25 (= P23), V26 (≠ L24), A28 (≠ V26), A66 (= A64), D67 (= D65), I68 (= I66), G104 (≠ A102), G105 (≠ R103), E108 (= E106), E128 (= E126), Y161 (≠ L159)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G308 (= G302), G310 (= G304), T311 (≠ H305), M312 (= M306), E331 (≠ D325), S332 (≠ R326), Q336 (≠ A330), A383 (= A381), V384 (≠ A382), F385 (= F383), E386 (= E384), L390 (≠ V388), K391 (= K389), N411 (= N409), S413 (= S411), H434 (= H432)
6z5oAAA Peroxisomal bifunctional enzyme (see paper)
40% identity, 98% coverage: 14:691/695 of query aligns to 17:705/716 of 6z5oAAA
- active site: A67 (= A64), F72 (≠ L69), G82 (≠ N79), G106 (≠ R103), E109 (= E106), P128 (= P125), E129 (= E126), G137 (= G134), K255 (= K252), S409 (= S411), H430 (= H432), E442 (= E444), N480 (= N482)
- binding coenzyme a: P26 (= P23), V27 (≠ L24), A65 (= A62), D68 (= D65), I69 (= I66), P128 (= P125), Y162 (≠ L159), F277 (= F274), K281 (≠ R278)
- binding nicotinamide-adenine-dinucleotide: G309 (= G302), G311 (= G304), T312 (≠ H305), M313 (= M306), E332 (≠ D325), S333 (≠ R326), Q337 (≠ A330), A379 (= A381), V380 (≠ A382), F381 (= F383), E382 (= E384), K387 (= K389), N407 (= N409), S409 (= S411), H430 (= H432)
- binding nicotinamide: A67 (= A64), E109 (= E106), E129 (= E126), P136 (= P133), F261 (= F258)
3zwbA Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with 2trans-hexenoyl-coa (see paper)
40% identity, 98% coverage: 14:691/695 of query aligns to 16:711/725 of 3zwbA
- active site: A66 (= A64), G81 (≠ N79), G105 (≠ R103), E108 (= E106), P127 (= P125), A128 (≠ E126), P135 (= P133), G136 (= G134), S415 (= S411), H436 (= H432), E448 (= E444), N486 (= N482)
- binding (2E)-Hexenoyl-CoA: P25 (= P23), V26 (≠ L24), A28 (≠ V26), A64 (= A62), G65 (= G63), A66 (= A64), D67 (= D65), I68 (= I66), V101 (≠ T99), L103 (≠ V101), G105 (≠ R103), E108 (= E106), G136 (= G134), Y161 (≠ L159), K280 (≠ R278)
P40939 Trifunctional enzyme subunit alpha, mitochondrial; 78 kDa gastrin-binding protein; Monolysocardiolipin acyltransferase; TP-alpha; EC 2.3.1.-; EC 4.2.1.17; EC 1.1.1.211 from Homo sapiens (Human) (see 5 papers)
31% identity, 98% coverage: 1:682/695 of query aligns to 36:750/763 of P40939
- V282 (≠ L214) to D: in MTPD1; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852773
- I305 (≠ V236) to N: in MTPD1; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852774
- L342 (≠ A273) to P: in LCHAD deficiency; dbSNP:rs137852772
- E510 (= E444) active site, For hydroxyacyl-coenzyme A dehydrogenase activity; to Q: in AFLP and LCHAD deficiency; loss of long-chain-3-hydroxyacyl-CoA dehydrogenase activity; dbSNP:rs137852769
6yswA E. Coli anaerobic trifunctional enzyme subunit-alpha in complex with coenzyme a
34% identity, 98% coverage: 12:693/695 of query aligns to 11:703/707 of 6yswA
- active site: A66 (= A64), I71 (≠ L69), A84 (vs. gap), Q88 (≠ N79), G112 (≠ R103), E115 (= E106), P136 (= P125), E137 (= E126), G145 (= G134), D264 (≠ K252), S422 (= S411), H443 (= H432), E455 (= E444), N493 (= N482)
- binding coenzyme a: E23 (vs. gap), M25 (≠ L24), A66 (= A64), D67 (= D65), I68 (= I66), P136 (= P125), E137 (= E126), L140 (≠ V129), T290 (≠ R278), K293 (≠ A281)
1wdlA Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form ii (native4) (see paper)
33% identity, 95% coverage: 29:685/695 of query aligns to 38:706/715 of 1wdlA
- active site: A69 (= A64), N89 (vs. gap), N93 (= N79), G117 (≠ R103), E120 (= E106), P139 (= P125), E140 (= E126), P147 (= P133), G148 (= G134), S430 (= S411), H451 (= H432), E463 (= E444), N501 (= N482)
- binding nicotinamide-adenine-dinucleotide: A322 (≠ S303), I324 (≠ H305), M325 (= M306), D344 (= D325), I345 (≠ R326), A400 (= A381), V401 (≠ A382), E403 (= E384), N428 (= N409), T429 (= T410), S430 (= S411)
P28793 Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Pseudomonas fragi (see paper)
33% identity, 95% coverage: 29:685/695 of query aligns to 38:706/715 of P28793
- D297 (≠ E277) binding substrate
- M325 (= M306) binding NAD(+)
- D344 (= D325) binding NAD(+)
- VVE 401:403 (≠ AFE 382:384) binding NAD(+)
- K408 (= K389) binding NAD(+)
- S430 (= S411) binding NAD(+)
- N454 (≠ S435) binding NAD(+)
- N501 (= N482) binding substrate
- Y660 (≠ N638) binding substrate
P21177 Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Escherichia coli (strain K12) (see 2 papers)
32% identity, 98% coverage: 11:694/695 of query aligns to 14:715/729 of P21177
- G116 (≠ R103) mutation to F: Absence of both enoyl-CoA hydratase and 3-hydroxyacyl-CoA epimerase activities. Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase is only slightly affected.
- G322 (= G304) mutation to A: 10-fold increase in KM for NADH.
- H450 (= H432) active site, For 3-hydroxyacyl-CoA dehydrogenase activity; mutation H->A,Q: Almost complete loss of 3-hydroxyacyl-CoA dehydrogenase activity.
6tnmA E. Coli aerobic trifunctional enzyme subunit-alpha (see paper)
32% identity, 98% coverage: 11:694/695 of query aligns to 14:715/719 of 6tnmA
- active site: A68 (= A64), F73 (≠ L69), G116 (≠ R103), E119 (= E106), P138 (= P125), E139 (= E126), G147 (= G134), N271 (≠ K252), S429 (= S411), H450 (= H432), E462 (= E444), N500 (= N482)
- binding adenosine-5'-triphosphate: D343 (= D325), I344 (≠ R326), V400 (≠ A382), V401 (≠ F383), V406 (= V388), K584 (= K561)
1wdmA Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form i (native3) (see paper)
33% identity, 95% coverage: 29:685/695 of query aligns to 38:698/707 of 1wdmA
- active site: A69 (= A64), N89 (vs. gap), N93 (= N79), G117 (≠ R103), E120 (= E106), P139 (= P125), E140 (= E126), P147 (= P133), G148 (= G134), S430 (= S411), H451 (= H432), E463 (= E444), N501 (= N482)
- binding acetyl coenzyme *a: K142 (= K128), D297 (≠ E277), M459 (≠ Q440), N501 (= N482), P534 (≠ A515), Y652 (≠ N638), L658 (≠ A644)
- binding nicotinamide-adenine-dinucleotide: G321 (= G302), A322 (≠ S303), I324 (≠ H305), M325 (= M306), D344 (= D325), V401 (≠ A382), E403 (= E384), N428 (= N409), S430 (= S411), N454 (≠ S435)
7o4uA Structure of the alpha subunit of mycobacterium tuberculosis beta- oxidation trifunctional enzyme in complex with oxidized nicotinamide adenine dinucleotide (see paper)
32% identity, 92% coverage: 12:652/695 of query aligns to 11:673/711 of 7o4uA
Query Sequence
>WP_013420474.1 NCBI__GCF_000166055.1:WP_013420474.1
MARSPVTYETKDGVGVITVDNPPLNVVSNEVRDGLMLAIRKAEADPVAKAVVLIGAGRNF
AAGADIYELTHPYSGADINDICAALEDLPKPVVAALHGTPVARGLEVALACHYRIAAKDA
RLGLPEVKVGLLPGGGGTQRLPRLVGAKAALDIIISGDLVPAVKAKTIGLVDEVVDGDIL
PAAIALAKSKSFLSKHPKTRELSAKIAPDRGSHLFEGRRDELRRRQPFQHAQFRCVDAIE
SAVHLPFSQGLKRERELFNEALDDDQSKALIHAFFAEREAAKIPGFRTDVRPREIKSVAV
VGSGHMGGGIAMAFANAGFPVIVLDRDQAALSRGLAVVAKNYESMVRRGRLDQPGMNKRL
SAIRSTVNYKDLADVDLVVEAAFEDIGVKEQVFRDLDRACKKGAIFASNTSTLDIDEIAS
FTRRPGDVIGTHFFSPANVQRLVEVVRGKETSADVLATAMAVAKKIGKIGVAVGNCDGFA
GNRMLEKYVTEAMIILEEGATPAEVDTALTRWGLAMGPFAVFDLTGIDVNWHIRQRRLKQ
GKPYGSALLDRFYDAGRFGQKTGKGFYRYETGSRAPLPDPEADAIIEAYRREVGPRVKSV
SEQEIVKRTIYALVNEGANILDEGVVYRSGDIDTIYINGYGFPAWRGGPMKYAELRGLAE
VLSDLDIFHMRFGDRWRPAALLKSLVTARKPKWPR
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory