SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013421190.1 NCBI__GCF_000166055.1:WP_013421190.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q5F8J4 Homoserine dehydrogenase; HDH; HSD; NAD(+)-dependent homoserine dehydrogenase; NAD(+)-dependent HSD; NgHSD; EC 1.1.1.3 from Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) (see paper)
44% identity, 95% coverage: 25:443/443 of query aligns to 22:433/435 of Q5F8J4

query
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Q5F8J4

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L45 (≠ R48) mutation to R: Shows a marked increase in the catalytic efficiency with NADP(+).
LS 45:46 (≠ RT 48:49) mutation to RD: Does not impair the catalytic activity with NAD(+). Slightly increases the activity, but slightly decreases the affinity for NADP(+).; mutation to RR: Causes a shift in coenzyme preference from NAD(+) to NADP(+) by a factor of 974. Shows a slight decrease in the catalytic efficiency with NAD(+) and a 4.5-fold increase in catalytic efficiency with NADP(+).

6dzsA Mycobacterial homoserine dehydrogenase thra in complex with NADP
37% identity, 99% coverage: 3:441/443 of query aligns to 1:429/431 of 6dzsA

query
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binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G13), L12 (= L14), G13 (= G15), N14 (≠ T16), V15 (= V17), V45 (≠ A47), R46 (= R48), R47 (≠ T49), R52 (= R54), I63 (≠ A67), L78 (= L82), M79 (≠ I83), P84 (= P89), A102 (= A107), K104 (= K109), G306 (= G306), T310 (= T310)

4pg7A Crystal structure of s. Aureus homoserine dehydrogenase at ph7.5 (see paper)
39% identity, 77% coverage: 4:346/443 of query aligns to 2:334/402 of 4pg7A

query
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4pg7A

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active site: D190 (= D205), K194 (= K209)
binding lysine: R215 (≠ E230), G219 (= G234)

Sites not aligning to the query:

binding lysine: 394, 395

6a0tB Homoserine dehydrogenase k99a mutant from thermus thermophilus hb8 complexed with hse and NADP+ (see paper)
39% identity, 63% coverage: 7:283/443 of query aligns to 4:269/332 of 6a0tB

query
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6a0tB

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S

F

G

G

R

V

S

V

I

P

E

R

V

F

A

L

G

G

V

V

S
x
L

A
x
V

R
|
R

T
x
D

P

P

G

R

K
|
K

D

P

R
|
R

G

A

V

I

D

P

L

Q

A

E

G

L

I

L

P

-

W

-

F

R

A

A

N

E

A

P

C

F

D

D

L

L

A

L

R

E

S

A

D

D

S

L

I

V

D

-

V

-

F

-

V

V

E

E

L
x
A

I
x
M

G
|
G

G

G

D

V

E

E

G

A

P

P

A

-

L

L

Q

R

S

L

V

V

E

L

V

P

A

A

L

L

E

E

A

A

G

G

K

I

H

P

V

L

V

I

T

T

A
|
A

N
|
N

K

K

A

A

L

L

A

A

R

E

H

A

G

W

V

E

Y

S

L

L

A

R

G

P

L

F

A

A

E

E

K

E

K

-

G

G

L

L

A

-

L

I

N

Y

F

H

E

E

A

A

A

S

I

V

A

M

G

A

G

G

I

T

P

P

I

A

V

L

K

S

G

F

L

L

R

-

E

E

S

T

L

L

L

R

G

G

N

S

S

E

I

L

S

L

R

E

V

L

Y

H

G

G

I

I

L

L

N
|
N

G
|
G

T
|
T

S

T

N

L

Y

Y

I

I

L

L

T

Q

R

E

M

M

Q

E

R

K

E

-

G

G

L

R

P

T

F

Y

A

A

E

E

V

A

L

L

A

L

E

E

A

A

Q

Q

A

R

L

L

G
|
G

Y
|
Y

A

A

E
|
E

A

A

D

D

P

P

T

T

F

L

D
|
D

V

V

D

E

G

G

Q

I

D
|
D

A

A

G

A

H

H

K
|
K

L

L

A

T

L

L

S

A

A

R

L

L

A

L

F

V

G

D

V

P

R

G

P

F

D

P

F

F

G

A

A

E

I

V

H

E

L

A

E

Q

G

G

I

I

R

A

G

R

I

L

T

T

P

P

D

E

D

V

F

L

D

Q

A

K

A

A

E

E

E

A

L

R

G

G

Y

E

R

R

I

V

K

R

L

L

L

V

A

A

V

S

A

L

I

F

Q

G

T

E

E

G

N

G

G

R

I

W

E

R

A

A

R

A

V

V

S

A

P

P

T

R

M

R

I

L

P

P

K

Q

T

D

S

H

A

P

L

L

A

A

G

R

V

A

D

R

G

G

active site: D191 (= D205), K195 (= K209)
binding l-homoserine: N150 (= N163), G151 (= G164), T152 (= T165), Y178 (= Y192), E180 (= E194), D186 (= D200), K195 (= K209)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G13), G11 (≠ L14), G12 (= G15), T13 (= T16), V14 (= V17), L42 (≠ S46), V43 (≠ A47), R44 (= R48), D45 (≠ T49), K48 (= K52), R50 (= R54), A73 (≠ L82), M74 (≠ I83), G75 (= G84), A97 (= A107), N98 (= N108), G177 (= G191), E180 (= E194)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 289, 290, 294

6a0sA Homoserine dehydrogenase from thermus thermophilus hb8 complexed with hse and NADPH (see paper)
39% identity, 63% coverage: 7:283/443 of query aligns to 4:269/331 of 6a0sA

query
sites
6a0sA

L

L

R

K

L

I

G

A

I

L

A

L

G

G

L
x
G

G
|
G

T
|
T

V
|
V

G

G

A

S

G

A

L

F

V

Y

S

N

L

L

L

V

R

L

E

E

N

R

G

A

A

E

I

L

A

S

D

A

R

-

S

F

G

G

R

V

S

V

I

P

E

R

V

F

A

L

G

G

V

V

S
x
L

A
x
V

R
|
R

T
x
D

P

P

G

R

K
|
K

D

P

R
|
R

G

A

V

I

D

P

L

Q

A

E

G

L

I

L

P

-

W

-

F

R

A

A

N

E

A

P

C

F

D

D

L

L

A

L

R

E

S

A

D

D

S

L

I

V

D

-

V

-

F

-

V

V

E

E

L
x
A

I
x
M

G

G

D

V

E

E

G

A

P

P

A

-

L

L

Q

R

S

L

V

V

E

L

V

P

A

A

L

L

E

E

A

A

G

G

K

I

H

P

V

L

V

I

T

T

A
|
A

N

N

K
|
K

A

A

L

L

A

A

R

E

H

A

G

W

V

E

Y

S

L

L

A

R

G

P

L

F

A

A

E

E

K

E

K

-

G

G

L

L

A

-

L

I

N

Y

F

H

E

E

A

A

A

S

I

V

A

M

G

A

G

G

I

T

P

P

I

A

V

L

K

S

G

F

L

L

R

-

E

E

S

T

L

L

L

R

G

G

N

S

S

E

I

L

S

L

R

E

V

L

Y

H

G

G

I

I

L

L

N
|
N

G
|
G

T
|
T

S

T

N

L

Y

Y

I

I

L

L

T

Q

R

E

M

M

Q

E

R

K

E

-

G

G

L

R

P

T

F

Y

A

A

E

E

V

A

L

L

A

L

E

E

A

A

Q

Q

A

R

L

L

G
|
G

Y
|
Y

A

A

E
|
E

A

A

D

D

P

P

T

T

F

L

D
|
D

V

V

D

E

G

G

Q

I

D
|
D

A

A

G

A

H

H

K
|
K

L

L

A

T

L

L

S

A

A

R

L

L

A

L

F

V

G

D

V

P

R

G

P

F

D

P

F

F

G

A

A

E

I

V

H

E

L

A

E

Q

G

G

I

I

R

A

G

R

I

L

T

T

P

P

D

E

D

V

F

L

D

Q

A

K

A

A

E

E

E

A

L

R

G

G

Y

E

R

R

I

V

K

R

L

L

L

V

A

A

V

S

A

L

I

F

Q

G

T

E

E

G

N

G

G

R

I

W

E

R

A

A

R

A

V

V

S

A

P

P

T

R

M

R

I

L

P

P

K

Q

T

D

S

H

A

P

L

L

A

A

G

R

V

A

D

R

G

G

active site: D191 (= D205), K195 (= K209)
binding l-homoserine: K99 (= K109), N150 (= N163), G151 (= G164), T152 (= T165), Y178 (= Y192), E180 (= E194), D186 (= D200), K195 (= K209)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (≠ L14), G12 (= G15), T13 (= T16), V14 (= V17), L42 (≠ S46), V43 (≠ A47), R44 (= R48), D45 (≠ T49), K48 (= K52), R50 (= R54), A73 (≠ L82), M74 (≠ I83), A97 (= A107), K99 (= K109), G177 (= G191), E180 (= E194)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 289, 290, 294

2ejwA Homoserine dehydrogenase from thermus thermophilus hb8
39% identity, 63% coverage: 7:283/443 of query aligns to 4:269/331 of 2ejwA

query
sites
2ejwA

L

L

R

K

L

I

G

A

I

L

A

L

G

G

L

G

G

G

T

T

V

V

G

G

A

S

G

A

L

F

V

Y

S

N

L

L

L

V

R

L

E

E

N

R

G

A

A

E

I

L

A

S

D

A

R

-

S

F

G

G

R

V

S

V

I

P

E

R

V

F

A

L

G

G

V

V

S

L

A

V

R

R

T

D

P

P

G

R

K

K

D

P

R

R

G

A

V

I

D

P

L

Q

A

E

G

L

I

L

P

-

W

-

F

R

A

A

N

E

A

P

C

F

D

D

L

L

A

L

R

E

S

A

D

D

S

L

I

V

D

-

V

-

F

-

V

V

E

E

L

A

I

M

G

G

D

V

E

E

G

A

P

P

A

-

L

L

Q

R

S

L

V

V

E

L

V

P

A

A

L

L

E

E

A

A

G

G

K

I

H

P

V

L

V

I

T

T

A

A

N

N

K

K

A

A

L

L

A

A

R

E

H

A

G

W

V

E

Y

S

L

L

A

R

G

P

L

F

A

A

E

E

K

E

K

-

G

G

L

L

A

-

L

I

N

Y

F

H

E
|
E

A

A

A

S

I
x
V

A
x
M

G
x
A

G

G

I
x
T

P

P

I

A

V

L

K

S

G

F

L

L

R

-

E

E

S

T

L

L

L

R

G

G

N

S

S

E

I

L

S

L

R

E

V

L

Y

H

G

G

I

I

L

L

N

N

G

G

T

T

S

T

N

L

Y

Y

I

I

L

L

T

Q

R

E

M

M

Q

E

R

K

E

-

G

G

L

R

P

T

F

Y

A

A

E

E

V

A

L

L

A

L

E

E

A

A

Q

Q

A

R

L

L

G

G

Y

Y

A

A

E

E

A

A

D

D

P

P

T

T

F

L

D

D

V

V

D

E

G

G

Q

I

D
|
D

A

A

G

A

H

H

K
|
K

L

L

A

T

L

L

S

A

A

R

L

L

A

L

F

V

G

D

V

P

R

G

P

F

D

P

F

F

G

A

A

E

I

V

H

E

L

A

E

Q

G

G

I

I

R

A

G

R

I

L

T

T

P

P

D

E

D

V

F

L

D

Q

A

K

A

A

E

E

E

A

L

R

G

G

Y

E

R

R

I

V

K

R

L

L

L

V

A

A

V

S

A

L

I

F

Q

G

T

E

E

G

N

G

G

R

I

W

E

R

A

A

R

A

V

V

S

A

P

P

T

R

M

R

I

L

P

P

K

Q

T

D

S

H

A

P

L

L

A

A

G

R

V

A

D

R

G

G

active site: D191 (= D205), K195 (= K209)
binding magnesium ion: E121 (= E133), V124 (≠ I136), M125 (≠ A137), A126 (≠ G138), T128 (≠ I140)

F9VNG5 Homoserine dehydrogenase; HDH; HSD; StHSD; EC 1.1.1.3 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 3 papers)
35% identity, 53% coverage: 89:323/443 of query aligns to 82:301/304 of F9VNG5

query
sites
F9VNG5

P

P

A

S

L

L

Q

S

S

L

V

Y

E

K

V

E

A

A

L

I

E

K

A

D

G

G

K

V

H

N

V

I

T

T

A
x
T

N

N

K
|
K

A

A

L

P

L

L

A

A

R

L

H

A

G

F

V

N

Y

E

L

I

A

F

G

S

L

L

A

A

E

R

K

S

K

K

G

G

L

V

A

K

L

I

N

G

F

F

E

Q

A

G

A

T

I

V

A

M

G

S

G

G

I

T

P

P

I

S

V

I

K

-

G

N

L

L

R

Y

E

R

S

V

L

L

L

P

G

G

N

S

S

R

I

V

S

I

R

K

V

I

Y

R

G

G

I

I

L

L

N

N

G

G

T

T

S

T

N

N

Y

F

I

I

L

L

T

T

R

L

M

M

Q

N

R

K

E

-

G

G

L

V

P

S

F

F

A

E

E

E

V

A

L

L

A

K

E

E

A

A

Q

Q

A

R

L

R

G
|
G

Y

Y

A

A

E
|
E

A

E

D

D

P

P

T

T

F

L

D

D

V

I

D

N

G

G

Q

F

D

D

A

A

G

A

H

A

K

K

L

I

A

T

L

I

L

L

S

A

A

N

L

F

A

M

F

I

G

G

V

N

R

S

P

V

D

T

F

I

G

K

A

D

I

V

H

K

L

F

E

E

G

G

I

I

R

N

G

R

I

D

T

L

P

P

D

K

D

N

F

-

D

-

A

-

E

-

L

-

G

-

Y

E

R

K

I

I

K

K

L

L

L

I

A

A

V

Y

A

A

I

D

Q

E

T

K

E

-

N

-

G

-

I

-

E

E

A

V

R

W

V

V

S

K

P

P

T

L

M

P

I

I

P

S

K

Q

T

D

S

D

A

P

L

L

A

Y

G

N

V

V

D

D

G

G

P

V

L

E

N

N

S

A

V

L

A

E

V

I

E

T

G

T

D

D

Y

I

S

Q

G

S

L

-

I

I

I

L

L

I

A

R

G

G

P

P

G

G

A

A

G
|
G

S

P

R

V

A

N

T

A

A

A

S

Y

S

G

V

A

A

L

S

S

D

D

I

L

I

I

D

L

I

L

A

K

R

R

T100 (≠ A107) binding NADP(+)
K102 (= K109) binding NADP(+)
G182 (= G191) binding NADP(+)
E185 (= E194) binding NADP(+)
G284 (= G306) binding NADP(+)

Sites not aligning to the query:

8 binding NADP(+)
10 binding NADP(+)
11 binding NADP(+)
38 binding NADP(+)
39 binding NADP(+)
73 binding NADP(+)
303 modified: Interchain

4xb2A Hyperthermophilic archaeal homoserine dehydrogenase mutant in complex with NADPH (see paper)
31% identity, 71% coverage: 7:319/443 of query aligns to 3:309/319 of 4xb2A

query
sites
4xb2A

L

V

R

N

L

I

G

S

I

I

A
x
F

G

G

L
x
F

G
|
G

T
|
T

V
|
V

G

G

A

R

G

A

L

L

V

A

S

E

L

I

R

A

E

E

-

K

-

S

-

R

-

I

-

F

-

G

-

V

-

E

-

L

N

N

G

V

A

I

A

S

I

I

A

T

D

D

R
|
R

S

S

G

G

R

T

-

I

-

W

-

G

-

D

-

F

S

D

I

L

E

L

V

E

A

A

G

K

V

E

S

V

A

A

R

E

T

S

P

T

G

G

K

K

D

L

R

S

G

N

V

I

D

G

L

D

A

Y

G

E

I

V

P

Y

W

N

F

F

A

S

N

-

A

P

C

Q

D

E

L

L

A

V

R

E

S

E

D

V

S

K

I

P

D

N

V

I

F

L

V

V

E

D

L
x
V

I
x
S

G
x
S

G

W

D

D

E

E

G

-

P

-

A

A

L

H

Q

E

S

M

V

Y

E

K

V

V

A

A

L

L

E

G

A

E

G

G

K

I

H

S

V

V

T

T

A
x
S

N
|
N

K
|
K

A

P

L

P

L

I

A

A

R

N

H

Y

G

Y

V

D

Y

E

L

L

A

M

G

N

L

L

A

A

E

K

K

E

K

N

G

N

L

A

A

G

L

I

N

F

F

F

E

E

A
x
S

A

T

I

V

A

M

G

A

G

G

I

T

P

P

I

I

V

I

K

G

G

V

L

L

R

R

E

E

S

N

L

L

G

G

N

E

S

N

I

I

S

K

R

R

V

I

Y

D

G

A

I

V

L

V

N

N

G
x
A

T
x
S

S

T

N

T

Y

F

I

I

L

L

T

T

R

K

M

M

Q

S

R

-

E

E

G

G

L

K

P

T

F

L

A

D

E

D

V

A

L

I

A

E

E

E

A

A

Q

K

A

S

L

L

G

G

Y

I

A

L

E

E

A

E

D

D

P

P

T

S

F

K

D
|
D

V

I

D

D

G

G

Q

I

D
|
D

A

A

G

Y

H

Y

K
|
K

L

A

A

K

L

I

L

L

S

H

A

W

L

V

A

S

F

Y

G

G

V

E

R

P

P

P

D

-

F

-

G

-

A

-

I

-

H

-

L

-

E

-

G

-

I

-

R

-

G

-

I

-

T

-

P

-

D

-

F

-

D

E

A

E

E

E

E

R

L

L

G

G

Y

I

R

R

-

E

-

V

-

R

-

D

-

A

-

R

-

N

I

V

K

R

L

L

L

V

A

A

V

-

A

-

I

-

Q

Q

T

V

E

S

N

K

G

G

I

-

E

K

A

I

R

S

V

V

S

K

P

P

T

R

M

K

I

L

P

S

K

S

T

D

S

N

A

P

L

L

A

L

G

-

V

V

D

E

G

G

P

V

L

Q

N

N

S

A

V

A

A

V

V

I

E

R

G

T

D

N

Y

N

S

L

G

G

L

E

I

V

I

I

L

L

A

K

G

G

P

P

G

G

A
x
G

G

G

S

G

R

R

A

V

T
|
T

A

A

S

S

S

G

V

V

A

F

S

T

D

D

I

I

active site: D211 (= D205), K215 (= K209)
binding l-homoserine: A171 (≠ G164), S172 (≠ T165), D206 (= D200), K215 (= K209)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F8 (≠ A12), F10 (≠ L14), G11 (= G15), T12 (= T16), V13 (= V17), R40 (= R35), V91 (≠ L82), S92 (≠ I83), S93 (≠ G84), S114 (≠ A107), N115 (= N108), K116 (= K109), S141 (≠ A134), G295 (≠ A305), T300 (= T310)

7f4cA The crystal structure of the immature holo-enzyme of homoserine dehydrogenase complexed with NADP and 1,4-butandiol from the hyperthermophilic archaeon sulfurisphaera tokodaii. (see paper)
34% identity, 53% coverage: 89:323/443 of query aligns to 82:297/300 of 7f4cA

query
sites
7f4cA

P

P

A

S

L

L

Q

S

S

L

V

Y

E

K

V

E

A

A

L

I

E

K

A

D

G

G

K

V

H

N

V

I

T

T

A
x
T

N

N

K
|
K

A

A

L

P

L

L

A

A

R

L

H

A

G

F

V

N

Y

E

L

I

A

F

G

S

L

L

A

A

E

R

K

S

K

K

G

G

L

V

A

K

L

I

N

G

F

F

E

Q

A
x
G

A

T

I

V

A

M

G
x
S

G

G

I

T

P

P

I

S

V

I

K

-

G

N

L

L

R

Y

E

R

S

V

L

L

L

P

G

G

N

S

S

R

I

V

S

I

R

K

V

I

Y

R

G

G

I

I

L

L

N

N

G

G

T

T

S

T

N

N

Y

F

I

I

L

L

T

T

R

L

M

M

Q

N

R

K

E

-

G

G

L

V

P

S

F

F

A

E

E

E

V

A

L

L

A

K

E

E

A

A

Q

Q

A

R

L

R

G

G

Y

Y

A

A

E
|
E

A

E

D

D

P

P

T

T

F

L

D

D

V

I

D

N

G

G

Q

F

D

D

A

A

G

A

H

A

K

K

L

I

A

T

L

I

L

L

S

A

A

N

L

F

A

M

F

I

G

G

V

N

R

S

P

V

D

T

F

I

G

K

A

D

I

V

H

K

L

F

E

E

G

G

I

I

R

N

G

R

I

-

T

-

P

-

D

-

F

-

D

-

A

-

E

-

L

-

G

D

Y

L

R

K

I

I

K

K

L

L

L

I

A

A

V

Y

A

A

I

D

Q

E

T

K

E

-

N

-

G

-

I

-

E

E

A

V

R

W

V

V

S

K

P

P

T

L

M

P

I

I

P

S

K

Q

T

D

S

D

A

P

L

L

A

Y

G

N

V

V

D

D

G

G

P

V

L

E

N

N

S

A

V

L

A

E

V

I

E

T

G

T

D

D

Y

I

S

Q

G

S

L

-

I

I

I

L

L

I

A

R

G

G

P

P

G

G

A

A

G
|
G

S

P

R

V

A

N

T
x
A

A

A

S

Y

S

G

V

A

A

L

S

S

D

D

I

L

I

I

D

L

I

L

A

K

R

R

binding nadp nicotinamide-adenine-dinucleotide phosphate: T100 (≠ A107), K102 (= K109), G127 (≠ A134), S131 (≠ G138), E185 (= E194), G280 (= G306), A284 (≠ T310)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 6, 7, 8, 9, 10, 11, 37, 38, 39, 72, 73, 74

4xb1A Hyperthermophilic archaeal homoserine dehydrogenase in complex with NADPH (see paper)
31% identity, 71% coverage: 7:319/443 of query aligns to 3:309/319 of 4xb1A

query
sites
4xb1A

L

V

R

N

L

I

G

S

I

I

A
x
F

G

G

L
x
F

G
|
G

T
|
T

V
|
V

G

G

A

R

G

A

L

L

V

A

S

E

L

I

R

A

E

E

-

K

-

S

-

R

-

I

-

F

-

G

-

V

-

E

-

L

N

N

G

V

A

I

A

S

I

I

A

T

D
|
D

R
|
R

S

S

G

G

R

T

S

I

-

W

-

G

-

D

-

F

-

D

-

L

I

L

E

E

V

A

A

K

G

E

V

V

S
x
K

A

E

R

S

T

T

P

-

G

G

K

K

D

L

R

S

G

N

V

I

D

G

L

D

A

Y

G

E

I

V

P

Y

W

N

F

F

A

S

N

-

A

P

C

Q

D

E

L

L

A

V

R

E

S

E

D

V

S

K

I

P

D

N

V

I

F

L

V

V

E

D

L
x
V

I
x
S

G
x
S

G

W

D

D

E

E

G

-

P

-

A

A

L

H

Q

E

S

M

V

Y

E

K

V

V

A

A

L

L

E

G

A

E

G

G

K

I

H

S

V

V

T

T

A
x
S

N

N

K
|
K

A

P

L

P

L

I

A

A

R

N

H

Y

G

Y

V

D

Y

E

L

L

A

M

G

N

L

L

A

A

E

K

K

E

K

N

G

N

L

A

A

G

L

I

N

F

F

F

E

E

A
x
S

A

T

I

V

A

M

G

A

G

G

I

T

P

P

I

I

V

I

K

G

G

V

L

L

R

R

E

E

S

N

L

L

G

G

N

E

S

N

I

I

S

K

R

R

V

I

Y

D

G

A

I

V

L

V

N

N

G

A

T

S

S

T

N

T

Y

F

I

I

L

L

T

T

R

K

M

M

Q

S

R

-

E

E

G

G

L

K

P

T

F

L

A

D

E

D

V

A

L

I

A

E

E

E

A

A

Q

K

A

S

L

L

G

G

Y

I

A

L

E

E

A

E

D

D

P

P

T

S

F

K

D

D

V

I

D

D

G

G

Q

I

D
|
D

A

A

G

Y

H

Y

K
|
K

L

A

A

K

L

I

L

L

S

H

A

W

L

V

A

S

F

Y

G

G

V

E

R

P

P

P

D

-

F

-

G

-

A

-

I

-

H

-

L

-

E

-

G

-

I

-

R

-

G

-

I

-

T

-

P

-

D

-

F

-

D

E

A

E

E

E

E

R

L

L

G

G

Y

I

R

R

-

E

-

V

-

R

-

D

-

A

-

R

-

N

I

V

K

R

L

L

L

V

A

A

V

-

A

-

I

-

Q

Q

T

V

E

S

N

K

G

G

I

-

E

K

A

I

R

S

V

V

S

K

P

P

T

R

M

K

I

L

P

S

K

S

T

D

S

N

A

P

L

L

A

L

G

-

V

V

D

E

G

G

P

V

L

Q

N

N

S

A

V

A

A

V

V

I

E

R

G

T

D

N

Y

N

S

L

G

G

L

E

I

V

I

I

L

L

A

K

G

G

P

P

G

G

A
x
G

G

G

S

G

R

R

A

V

T
|
T

A

A

S

S

S

G

V

V

A

F

S

T

D

D

I

I

active site: D211 (= D205), K215 (= K209)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F8 (≠ A12), F10 (≠ L14), G11 (= G15), T12 (= T16), V13 (= V17), D39 (= D34), R40 (= R35), K57 (≠ S46), V91 (≠ L82), S92 (≠ I83), S93 (≠ G84), S114 (≠ A107), K116 (= K109), S141 (≠ A134), G295 (≠ A305), T300 (= T310)

O58802 Homoserine dehydrogenase; HDH; HSD; HseDH; EC 1.1.1.3 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see paper)
31% identity, 71% coverage: 7:319/443 of query aligns to 3:309/319 of O58802

query
sites
O58802

L

V

R

N

L

I

G

S

I

I

A

F

G

G

L
x
F

G

G

T
|
T

V
|
V

G

G

A

R

G

A

L

L

V

A

S

E

L

I

R

A

E

E

-

K

-

S

-

R

-

I

-

F

-

G

-

V

-

E

-

L

N

N

G

V

A

I

A

S

I

I

A

T

D

D

R
|
R

S

S

G

G

R

T

S

I

-

W

-

G

-

D

-

F

-

D

-

L

I

L

E

E

V

A

A

K

G

E

V

V

S
x
K

A

E

R

S

T

T

P

-

G

G

K

K

D

L

R

S

G

N

V

I

D

G

L

D

A

Y

G

E

I

V

P

Y

W

N

F

F

A

S

N

-

A

P

C

Q

D

E

L

L

A

V

R

E

S

E

D

V

S

K

I

P

D

N

V

I

F

L

V

V

E

D

L

V

I
x
S

G
x
S

G

W

D

D

E

E

G

-

P

-

A

A

L

H

Q

E

S

M

V

Y

E

K

V

V

A

A

L

L

E

G

A

E

G

G

K

I

H

S

V

V

T

T

A
x
S

N

N

K
|
K

A

P

L

P

L

I

A

A

R

N

H

Y

G

Y

V

D

Y

E

L

L

A

M

G

N

L

L

A

A

E

K

K

E

K

N

G

N

L

A

A

G

L

I

N

F

F

F

E
|
E

A

S

A

T

I
x
V

A

M

G
x
A

G

G

I
x
T

P

P

I

I

V

I

K

G

G

V

L

L

R

R

E

E

S

N

L

L

G

G

N

E

S

N

I

I

S

K

R

R

V

I

Y

D

G

A

I

V

L

V

N

N

G

A

T

S

S

T

N

T

Y

F

I

I

L

L

T

T

R

K

M

M

Q

S

R

-

E

E

G

G

L

K

P

T

F

L

A

D

E

D

V

A

L

I

A

E

E

E

A

A

Q

K

A

S

L

L

G

G

Y

I

A

L

E

E

A

E

D

D

P

P

T

S

F

K

D

D

V

I

D

D

G

G

Q

I

D

D

A

A

G

Y

H

Y

K

K

L

A

A

K

L

I

L

L

S

H

A

W

L

V

A

S

F

Y

G

G

V

E

R

P

P

P

D

-

F

-

G

-

A

-

I

-

H

-

L

-

E

-

G

-

I

-

R

-

G

-

I

-

T

-

P

-

D

-

F

-

D

E

A

E

E

E

E

R

L

L

G

G

Y

I

R

R

-

E

-

V

-

R

-

D

-

A

-

R

-

N

I

V

K

R

L

L

L

V

A

A

V

-

A

-

I

-

Q

Q

T

V

E

S

N

K

G

G

I

-

E

K

A

I

R

S

V

V

S

K

P

P

T

R

M

K

I

L

P

S

K

S

T

D

S

N

A

P

L

L

A

L

G

-

V

V

D

E

G

G

P

V

L

Q

N

N

S

A

V

A

A

V

V

I

E

R

G

T

D

N

Y

N

S

L

G

G

L

E

I

V

I

I

L

L

A

K

G

G

P

P

G

G

A

G

G
|
G

S

G

R

R

A

V

T

T

A

A

S

S

S

G

V

V

A

F

S

T

D

D

I

I

F10 (≠ L14) binding NADPH
T12 (= T16) binding NADPH
V13 (= V17) binding NADPH
R40 (= R35) binding NADPH; mutation to A: Increases activity with NADP.
K57 (≠ S46) binding NADPH; mutation to A: Increases activity with NADP.
S92 (≠ I83) binding NADPH
S93 (≠ G84) binding NADPH
S114 (≠ A107) binding NADPH
K116 (= K109) binding NADPH
E140 (= E133) binding Na(+)
V143 (≠ I136) binding Na(+)
A145 (≠ G138) binding Na(+)
T147 (≠ I140) binding Na(+)
G296 (= G306) binding NADPH

5x9dA Crystal structure of homoserine dehydrogenase in complex with l- cysteine and NAD (see paper)
35% identity, 53% coverage: 89:323/443 of query aligns to 82:299/302 of 5x9dA

query
sites
5x9dA

P
|
P

A

S

L

L

Q

S

S

L

V

Y

E

K

V

E

A

A

L

I

E

K

A

D

G

G

K

V

H

N

V

I

T

T

A
x
T

N
|
N

K
|
K

A

A

L

P

L

L

A

A

R

L

H

A

G

F

V

N

Y

E

L

I

A

F

G

S

L

L

A

A

E

R

K

S

K

K

G

G

L

V

A

K

L

I

N

G

F

F

E

Q

A
x
G

A

T

I

V

A

M

G
x
S

G

G

I

T

P

P

I

S

V

I

K

-

G

N

L

L

R

Y

E

R

S

V

L

L

L

P

G

G

N

S

S

R

I

V

S

I

R

K

V

I

Y

R

G

G

I

I

L

L

N
|
N

G
|
G

T
|
T

S

T

N

N

Y

F

I

I

L

L

T

T

R

L

M

M

Q

N

R

K

E

-

G

G

L

V

P

S

F

F

A

E

E

E

V

A

L

L

A

K

E

E

A

A

Q

Q

A

R

L

R

G

G

Y
|
Y

A
|
A

E
|
E

A

E

D

D

P

P

T

T

F

L

D
|
D

V

I

D

N

G

G

Q

F

D
|
D

A

A

G

A

H

A

K
|
K

L

I

A

T

L

I

L

L

S

A

A

N

L

F

A

M

F

I

G

G

V

N

R

S

P

V

D

T

F

I

G

K

A

D

I

V

H

K

L

F

E

E

G

G

I

I

R

-

G

-

I

-

T

-

P

-

D

-

D

N

F

R

D

D

A

P

A

K

E

N

E

E

L

-

G

-

Y

-

R

K

I

I

K

K

L

L

L

I

A

A

V

Y

A

A

I

D

Q

E

T

K

E

-

N

-

G

-

I

-

E

E

A

V

R

W

V

V

S

K

P

P

T

L

M

P

I

I

P

S

K

Q

T

-

S

D

A

P

L

L

A

Y

G

N

V

V

D

D

G

G

P

V

L

E

N

N

S

A

V

L

A

E

V

I

E

T

G

T

D

D

Y

I

S

Q

G

S

L

-

I

I

I

L

L

I

A

R

G

G

P

P

G

G

A
|
A

G
|
G

S

P

R

V

A

N

T
x
A

A

A

S

Y

S

G

V

A

A

L

S

S

D

D

I

L

I

I

D

L

I

L

A

K

R

R

active site: D196 (= D205), K200 (= K209)
binding (2R)-3-[[(4S)-3-aminocarbonyl-1-[(2R,3R,4S,5R)-5-[[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-3,4-bis(oxidanyl)oxolan-2-yl]-4H-pyridin-4-yl]sulfanyl]-2-azanyl-propanoic acid: P82 (= P89), T100 (≠ A107), N101 (= N108), K102 (= K109), G127 (≠ A134), S131 (≠ G138), N155 (= N163), G156 (= G164), T157 (= T165), Y183 (= Y192), A184 (= A193), E185 (= E194), D191 (= D200), D196 (= D205), K200 (= K209), A281 (= A305), G282 (= G306), A286 (≠ T310)

Sites not aligning to the query:

binding (2R)-3-[[(4S)-3-aminocarbonyl-1-[(2R,3R,4S,5R)-5-[[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-3,4-bis(oxidanyl)oxolan-2-yl]-4H-pyridin-4-yl]sulfanyl]-2-azanyl-propanoic acid: 6, 7, 8, 9, 10, 11, 37, 38, 72, 73, 74

O81852 Bifunctional aspartokinase/homoserine dehydrogenase 2, chloroplastic; AK-HD 2; AK-HSDH 2; Beta-aspartyl phosphate homoserine 2; EC 2.7.2.4; EC 1.1.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
27% identity, 71% coverage: 7:322/443 of query aligns to 558:909/916 of O81852

query
sites
O81852

L

L

R

A

L

M

G

G

I

I

A

V

G

G

L

P

G

G

T

L

V

I

G

G

A

A

G

T

L

L

V

L

S

D

L

Q

L

L

R

R

E

D

N

Q

G

A

A

A

A

V

I

L

A

K

D

Q

R

E

S

F

G

N

R

I

S

D

I

L

E

R

V

V

A

L

G

G

V

I

-

T

-

G

S

S

A

K

R

K

T

M

P

L

G

L

K

S

D

D

R

I

G

G

V

I

D

D

L

L

A

S

G

R

I

-

P

-

W

W

F

R

A

E

N

L

A

L

C

N

D

E

L

K

A

G

R

T

S

E

D

A

S

D

I

L

D

D

V

K

F

F

V

T

E

Q

L

Q

I

V

G

H

G

G

D

N

E

H

G

F

-

I

P

P

A

N

L

S

Q

V

S

V

V

V

E

D

V

C

A

T

-

A

-

D

-

S

-

A

-

I

-

A

-

S

-

R

-

Y

-

D

-

W

L

L

E

R

A

K

G

G

K

I

H

H

V

V

V

I

T

T

A

P

N

N

K

K

A

K

L

-

A

A

R

N

H

S

G

G

-

P

-

L

-

D

-

Q

-

Y

V

L

Y

K

L

L

A

R

G

D

L

L

A

Q

E

R

K

K

K

S

G

Y

L

T

A

H

L

Y

N

F

F

Y

E

E

A

A

A

T

I

V

A

G

G

A

G

G

I

L

P

P

I

I

V

I

K

S

G

T

L

L

R

R

E

G

S

L

L

L

-

E

L

T

G

G

N

D

S

K

I

I

S

L

R

R

V

I

Y

E

G

G

I

I

L

C

N

S

G

G

T

T

S

L

N

S

Y

Y

I

L

L

F

T

N

R

N

M

F

Q

V

R

G

E

D

G

R

L

-

P

S

F

F

A

S

E

E

V

V

L

V

A

T

E

E

A

A

Q

K

A

N

L

A

G

G

Y

F

A

T

E

E

A

P

D

D

P

P

T

R

F

D

D

D

V

L

D

S

G

G

Q

T

D

D

A

V

G

A

H

R

K

K

L

V

A

I

L

I

L

L

S

-

A

A

L

R

A

E

F

S

G

G

V

L

R

K

P

L

D

D

F

L

G

A

A

D

I

L

H

P

L

I

-

R

-

S

-

L

-

V

-

P

E

E

G

P

I

L

R

K

G

G

I

C

T

T

P

S

D

V

D

E

-

E

-

F

-

M

-

E

-

K

-

L

-

P

-

Q

F

Y

D

D

A

G

-

D

-

L

-

A

-

K

-

E

-

R

-

L

-

D

A

A

E

E

E

N

L

S

G

G

Y

E

R

V

I

L

K

R

L

Y

L

V

A

G

V

V

-

V

-

D

A

A

I

V

Q

N

T

Q

E

K

N

G

G

T

I

V

E

E

A

L

R

R

V

R

S

Y

P

K

T

K

M

E

I

H

P

P

K

-

T

F

S

A

A

Q

L

L

A

A

G

G

V

S

D

D

G

N

P

I

L

I

N

A

S

F

V

T

A

T

V

T

E

R

G

Y

D

K

Y

D

S

H

G

P

L

L

I

I

I

V

L

-

A

R

G

G

P

P

G

G

A

A

G

G

S

A

R

Q

A

V

T

T

A

A

S

G

V

I

A

F

S

S

D

D

I

I

I

L

D

R

I

L

A

A

Sites not aligning to the query:

441 I→A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
443 Q→A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
522 I→A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.
524 Q→A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.

3ingA Crystal structure of homoserine dehydrogenase (np_394635.1) from thermoplasma acidophilum at 1.95 a resolution
28% identity, 51% coverage: 7:232/443 of query aligns to 3:236/319 of 3ingA

query
sites
3ingA

L

I

R

R

L

I

G

I

I

L

A

M

G
|
G

L
x
T

G
|
G

T
x
N

V
|
V

G

G

A

L

G

N

L

V

V

L

S

R

L

I

R

D

E

A

N

-

G

-

A

-

I

-

A

S

D

N

R

R

S

R

G

R

R

F

S

S

I

I

E

K

V

V

A

V

G

G

V

V

S

S

-
x
D

A
x
S

R

R

T

S

P

Y

G

A

K

S

D

G

R

R

G

N

V

L

D

D

L

I

A

S

G

S

I

I

-

I

-

S

-

N

-
x
K

-

E

-

K

-

T

-

G

-

R

-

I

-

S

-

D

P

R

W

A

F

F

A

S

N

G

A

P

C

E

D

D

L

L

A

M

R

-

S

G

D

E

S

A

I

A

D

D

V

L

F

L

V

V

E

D

L
x
C

I
x
T

-
x
P

G

A

G

S

D

R

E

D

G

G

P

V

A

R

L

E

Q

Y

S

S

V

L

-

Y

E

R

V

M

A

A

L

F

E

E

A

S

G

G

K

M

H

N

V

V

T

T

A
|
A

N
|
N

K
|
K

A

S

L

G

L

L

A

A

R

N

H

K

G

W

V

H

Y

D

L

I

A

M

G

D

L

S

A

A

E

N

K

Q

K

N

G

S

L

K

A

Y

L

I

N

R

F

Y

E

E

A
|
A

A

T

I

V

A

A

G

G

I

V

P

P

I

L

V

F

K

S

G

V

L

L

R

D

E

Y

S

S

L

I

L

L

G

P

N

S

S

K

I

V

S

K

R

R

V

F

Y

R

G

G

I

I

L

V

N

S

G

S

T

T

S

I

N

N

Y

Y

I

V

L

I

T

R

R

N

M

M

Q

A

R

-

E

N

G

G

L

R

P

S

F

L

A

R

E

D

V

V

L

V

A

D

E

D

A

A

Q

I

A

K

L

K

G

G

Y

I

A

A

E
|
E

A
x
S

D

N

P

P

T

Q

F

D

D

D

V

L

D

N

G

G

Q

L

D
|
D

A

A

G

A

H

R

K
|
K

L

S

A

V

L

I

L

L

S

V

A

N

L

H

A

I

F

F

G

G

V

T

R

E

P

Y

D

T

F

L

G

N

A

D

I

V

H

E

L

Y

E

S

G

G

I

V

active site: D209 (= D205), K213 (= K209)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G9 (= G13), T10 (≠ L14), G11 (= G15), N12 (≠ T16), V13 (= V17), D38 (vs. gap), S39 (≠ A47), K57 (vs. gap), C85 (≠ L82), T86 (≠ I83), P87 (vs. gap), A112 (= A107), N113 (= N108), K114 (= K109), A139 (= A134), E198 (= E194), S199 (≠ A195)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 302, 305

O94671 Homoserine dehydrogenase; HDH; HSD; EC 1.1.1.3 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
25% identity, 48% coverage: 7:217/443 of query aligns to 8:239/376 of O94671

query
sites
O94671

L

V

R

N

L

V

G

A

I

I

A

V

G

G

L

T

G

G

T

N

V

I

G

G

A

G

G

E

L

L

V

L

S

N

L

Q

L

I

R

K

-

G

-

F

-

N

E

E

N

N

G

A

A

S

A

T

I

N

A

G

D

T

R

T

S

S

G

F

R

N

S

V

I

V

E

A

V

I

A

S

G

S

V

M

S

E

A

G

R

H

T

Y

P

V

G

S

K

K

D

D

-

Y

R

Q

G

P

V

I

D

N

L

L

A

S

G

-

I

-

P

E

W

W

F

K

A

N

N

L

A

T

C

S

D

Q

L

N

A

Q

R

G

S

A

D

Y

S

S

I

L

D

D

V

A

F

L

V

V

E

D

L

F

I

L

G

A

G

K

D

S

E

P

G

L

P

P

A

A

L

I

-

L

-

V

-

D

-

N

-

T

-

A

-

S

-

E

-

A

-

I

-

A

Q

Q

S

S

V

Y

E

P

V

K

A

F

L

L

E

S

A

K

G

K

K

I

H

N

V

I

V

A

T

T

A

P

N

N

K

K

-

K

A

A

L

F

L

S

A

A

R

S

H

N

G

D

V

V

Y

Y

-

Q

-

N

L

I

A

I

G

K

L

A

A

S

E

K

K

E

K

S

G

G

L

A

A

L

L

L

N

M

F

H

E

E

A

A

A

S

I

V

A

G

G

A

G

G

I

L

P

P

I

I

V

I

K

S

G

T

L

L

R

K

E

E

S

L

L

I

-

A

L

T

G

G

N

D

S

E

I

I

S

I

R

K

V

I

Y

E

G

G

I

I

L

F

N

S

G

G

T

T

S

L

N

S

Y

Y

I

I

L

F

T

N

R

V

M

W

Q

S

R

P

E

N

G

G

L

K

P

K

-

G

-

T

-

A

-
x
S

F

F

A

S

E

D

V

I

L

V

A

K

E

I

A

A

Q

K

A

Q

L

N

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

T

R

F

D

D

D

V

L

D

N

G

G

Q

M

D

D

A

V

G

A

H

R

K

K

L

V

A

T

L

I

L

L

S

S

A

R

L

I

A

A

S201 (vs. gap) modified: Phosphoserine

3jsaA Homoserine dehydrogenase from thermoplasma volcanium complexed with NAD
27% identity, 52% coverage: 4:232/443 of query aligns to 1:239/321 of 3jsaA

query
sites
3jsaA

L

M

P

K

P

T

L

I

R

N

L

L

G

S

I

I

A

F

G
|
G

L
|
L

G
|
G

T
x
N

V
|
V

G

G

A

L

G

N

L

L

V

L

S

R

L

I

R

R

E

S

-

F

-

N

-

E

-

N

-

R

-

L

-

G

-

L

-

K

-

F

N

N

G

V

A

V

A

F

I

V

A

A

D
|
D

-
x
S

-

L

-

H

-

S

R

Y

S

Y

G

N

R

E

S

R

I

I

E

D

V

I

A

G

G

K

V

V

S

I

A

S

R

Y

T

-

P

-

G

-

K

K

D

E

R

K

G

G

V

-

D

S

L

L

A

D

G

S

I

L

P

E

W

Y

F

E

A

S

N

I

A

S

C

A

D

S

L

E

A

A

R

L

S

A

D

R

S

D

I

F

D

D

V

I

F

V

V

V

E

D

L
x
A

I
x
T

-
x
P

-

A

G

S

G

A

D

D

E

G

G

K

P

K

A

E

L

L

Q

A

S

F

V

Y

E

K

V

E

A

T

L

F

E

E

A

N

G

G

K

K

H

D

V

V

T

T

A
|
A

N
|
N

K

K

A

S

L

G

L

L

A

A

R

N

H

F

G

W

V

P

Y

E

L

I

A

M

G

E

L

Y

A

A

E

R

K

S

K

N

G

N

L

R

A

R

L

I

N

R

F

Y

E

E

A
|
A

A

T

I

V

A

A

G

G

I

V

P

P

I

L

V

F

K

S

G

F

L

I

R

D

E

Y

S

S

L

V

L

L

G

P

N

S

S

R

I

I

S

K

R

K

V

F

Y

R

G

G

I

I

L

V

N

S

G

L

T

T

S

I

N

N

Y

Y

I

F

L

I

T

R

R

E

M

L

-

A

-

N

Q

K

R

R

E

E

G

-

L

-

P

-

F

F

A

D

E

D

V

V

L

L

A

S

E

E

A

A

Q

T

A

K

L

L

G

G

Y

I

A

V

E

N

A

Y

D

K

P

D

T

-

F

-

D

D

V

L

D

T

G

G

Q

L

D
|
D

A

A

G

A

H

R

K
|
K

L

S

A

V

L

I

L

L

S

C

A

N

L

H

A

L

F

Y

G

G

V

S

R

S

P

Y

D

R

F

L

G

S

A

D

I

V

H

F

L

Y

E

E

G

G

I

I

active site: D212 (= D205), K216 (= K209)
binding nicotinamide-adenine-dinucleotide: G10 (= G13), L11 (= L14), G12 (= G15), N13 (≠ T16), V14 (= V17), D42 (= D34), S43 (vs. gap), A90 (≠ L82), T91 (≠ I83), P92 (vs. gap), A117 (= A107), N118 (= N108), A144 (= A134)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 308

1tveA Homoserine dehydrogenase in complex with 4-(4-hydroxy-3- isopropylphenylthio)-2-isopropylphenol (see paper)
30% identity, 26% coverage: 98:213/443 of query aligns to 105:226/358 of 1tveA

query
sites
1tveA

L

V

E

E

A

N

G

G

K

I

H

S

V

I

V

A

T

T

A

P

N

N

K
|
K

A

K

L

A

L

F

A

S

R

S

H

D

G

L

V

A

Y

T

L

W

A

K

G

A

L

L

A

F

E

S

K

N

K

K

-

P

-

T

-

N

G

G

L

F

A

V

L

Y

N

H

F

-

E

E

A

A

A

T

I

V

A

G

G

A

G

G

I

L

P

P

I

I

V

I

K

S

G

F

L

L

R

R

E

E

S

I

L

I

-

Q

L

T

G

G

N

D

S

E

I

V

S

E

R

K

V

I

Y

E

G

G

I

I

L

F

N

S

G

G

T
|
T

S

L

N

S

Y

Y

I

I

L

F

T

N

R

E

M

F

-

S

-

T

-

S

Q

Q

R

A

E

N

G

D

L

V

P

K

F

F

A

S

E

D

V

V

L

V

A

K

E

V

A

A

Q

K

A

K

L

L

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

T

R

F

D

D
|
D

V

L

D

N

G

G

Q

L

D
|
D

A

V

G

A

H

R

K
|
K

L

V

A

T

L

I

L

V

active site: D218 (= D205), K222 (= K209)
binding 4-(4-hydroxy-3-isopropylphenylthio)-2-isopropylphenol: K116 (= K109), T175 (= T165), D213 (= D200), D218 (= D205)

Sites not aligning to the query:

binding 4-(4-hydroxy-3-isopropylphenylthio)-2-isopropylphenol: 93

1q7gA Homoserine dehydrogenase in complex with suicide inhibitor complex NAD-5-hydroxy-4-oxonorvaline (see paper)
30% identity, 26% coverage: 98:213/443 of query aligns to 105:226/358 of 1q7gA

query
sites
1q7gA

L

V

E

E

A

N

G

G

K

I

H

S

V

I

V

A

T

T

A
x
P

N

N

K
|
K

A

K

L

A

L

F

A

S

R

S

H

D

G

L

V

A

Y

T

L

W

A

K

G

A

L

L

A

F

E

S

K

N

K

K

-

P

-

T

-

N

G

G

L

F

A

V

L

Y

N

H

F

-

E

E

A
|
A

A

T

I

V

A

G

G

A

G

G

I

L

P

P

I

I

V

I

K

S

G

F

L

L

R

R

E

E

S

I

L

I

-

Q

L

T

G

G

N

D

S

E

I

V

S

E

R

K

V

I

Y

E

G

G

I

I

L

F

N
x
S

G

G

T

T

S

L

N

S

Y

Y

I

I

L

F

T

N

R

E

M

F

-

S

-

T

-

S

Q

Q

R

A

E

N

G

D

L

V

P

K

F

F

A

S

E

D

V

V

L

V

A

K

E

V

A

A

Q

K

A

K

L

L

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

T

R

F

D

D

D

V

L

D

N

G

G

Q

L

D
|
D

A

V

G

A

H

R

K
|
K

L

V

A

T

L

I

L

V

active site: D218 (= D205), K222 (= K209)
binding nicotinamide-adenine-dinucleotide-5-hydroxy-4-oxonorvaline: P114 (≠ A107), K116 (= K109), A143 (= A134), S173 (≠ N163), K222 (= K209)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide-5-hydroxy-4-oxonorvaline: 13, 14, 15, 39, 91, 92, 93, 97, 338, 343

1ebuD Homoserine dehydrogenase complex with NAD analogue and l-homoserine (see paper)
30% identity, 26% coverage: 98:213/443 of query aligns to 105:226/358 of 1ebuD

query
sites
1ebuD

L

V

E

E

A

N

G

G

K

I

H

S

V

I

V

A

T

T

A

P

N

N

K
|
K

A

K

L

A

L

F

A

S

R

S

H

D

G

L

V

A

Y

T

L

W

A

K

G

A

L

L

A

F

E

S

K

N

K

K

-

P

-

T

-

N

G

G

L

F

A

V

L

Y

N

H

F

-

E

E

A

A

A

T

I

V

A

G

G

A

G

G

I

L

P

P

I

I

V

I

K

S

G

F

L

L

R

R

E

E

S

I

L

I

-

Q

L

T

G

G

N

D

S

E

I

V

S

E

R

K

V

I

Y

E

G

G

I

I

L

F

N

S

G

G

T

T

S

L

N

S

Y

Y

I

I

L

F

T

N

R

E

M

F

-

S

-

T

-

S

Q

Q

R

A

E

N

G

D

L

V

P

K

F

F

A

S

E

D

V

V

L

V

A

K

E

V

A

A

Q

K

A

K

L

L

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

T

R

F

D

D

D

V

L

D

N

G

G

Q

L

D
|
D

A

V

G

A

H

R

K
|
K

L

V

A

T

L

I

L

V

active site: D218 (= D205), K222 (= K209)
binding 3-aminomethyl-pyridinium-adenine-dinucleotide: K116 (= K109)

Sites not aligning to the query:

binding 3-aminomethyl-pyridinium-adenine-dinucleotide: 11, 12, 13, 14, 15, 39, 40, 91, 93, 343

1ebfA Homoserine dehydrogenase from s. Cerevisiae complex with NAD+ (see paper)
30% identity, 26% coverage: 98:213/443 of query aligns to 105:226/358 of 1ebfA

query
sites
1ebfA

L

V

E

E

A

N

G

G

K

I

H

S

V

I

V

A

T

T

A
x
P

N

N

K

K

A

K

L

A

L

F

A

S

R

S

H

D

G

L

V

A

Y

T

L

W

A

K

G

A

L

L

A

F

E

S

K

N

K

K

-

P

-

T

-

N

G

G

L

F

A

V

L

Y

N

H

F

-

E

E

A

A

A

T

I

V

A

G

G

A

G

G

I

L

P

P

I

I

V

I

K

S

G

F

L

L

R

R

E

E

S

I

L

I

-

Q

L

T

G

G

N

D

S

E

I

V

S

E

R

K

V

I

Y

E

G

G

I

I

L

F

N

S

G

G

T

T

S

L

N

S

Y

Y

I

I

L

F

T

N

R

E

M

F

-

S

-

T

-

S

Q

Q

R

A

E

N

G

D

L

V

P

K

F

F

A

S

E

D

V

V

L

V

A

K

E

V

A

A

Q

K

A

K

L

L

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

T

R

F

D

D

D

V

L

D

N

G

G

Q

L

D
|
D

A

V

G

A

H

R

K
|
K

L

V

A

T

L

I

L

V

active site: D218 (= D205), K222 (= K209)
binding nicotinamide-adenine-dinucleotide: P114 (≠ A107)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 10, 12, 13, 14, 15, 39, 40, 92, 93

Query Sequence

>WP_013421190.1 NCBI__GCF_000166055.1:WP_013421190.1
MTDLPPLRLGIAGLGTVGAGLVSLLRENGAAIADRSGRSIEVAGVSARTPGKDRGVDLAG
IPWFANACDLARSDSIDVFVELIGGDEGPALQSVEVALEAGKHVVTANKALLARHGVYLA
GLAEKKGLALNFEAAIAGGIPIVKGLRESLLGNSISRVYGILNGTSNYILTRMQREGLPF
AEVLAEAQALGYAEADPTFDVDGQDAGHKLALLSALAFGVRPDFGAIHLEGIRGITPDDF
DAAEELGYRIKLLAVAIQTENGIEARVSPTMIPKTSALAGVDGPLNSVAVEGDYSGLIIL
AGPGAGSRATASSVASDIIDIARGHISPPFILPVAKLRQQTRPGMFSHEGGFYIRLSVFD
RPGAIAAIATRMAEQQISLESVVQKRAPLDLPGISRAGQAGEPTPVVMITHRTREERLRS
ALAAIVEDGHVSGVPQTIRIEAL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory