SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013430763.1 NCBI__GCF_000166775.1:WP_013430763.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P12282 Molybdopterin-synthase adenylyltransferase; MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase; Sulfur carrier protein MoaD adenylyltransferase; EC 2.7.7.80 from Escherichia coli (strain K12) (see 2 papers)
25% identity, 94% coverage: 11:224/228 of query aligns to 18:234/249 of P12282

query
sites
P12282

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C44 (≠ G37) mutation to A: No effect.
R73 (= R66) mutation to A: No effect. No activity; when associated with A-14.; mutation to K: Substantially reduced activity.
C128 (≠ A121) mutation to A: No effect.; mutation to Y: No activity.
D130 (= D123) mutation to A: No activity.; mutation to E: Substantially reduced activity.
C142 (≠ A135) mutation to A: No effect.
C172 (vs. gap) mutation to A: No zinc bound and no enzyme activity.
C175 (≠ G163) mutation to A: No zinc bound and no enzyme activity.
C187 (≠ G175) mutation to A: No effect.
C231 (≠ N221) mutation to A: No effect.

Sites not aligning to the query:

14 mutation R->A,K: No effect.; R→A: No activity; when associated with A-73.
244 C→A: No zinc bound and almost no enzyme activity.
247 C→A: No zinc bound and almost no enzyme activity.

P30138 Sulfur carrier protein ThiS adenylyltransferase; EC 2.7.7.73 from Escherichia coli (strain K12) (see 3 papers)
27% identity, 67% coverage: 6:158/228 of query aligns to 8:162/251 of P30138

query
sites
P30138

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Sites not aligning to the query:

169 binding Zn(2+)
172 binding Zn(2+)
174 W→A: No adenylation of ThiS.
184 C→S: No cross-link formed with ThiS. No effect on ThiS thiocarboxylate formation in vitro. Does not support growth.
240 binding Zn(2+)
243 binding Zn(2+)

1zud3 Structure of this-thif protein complex (see paper)
27% identity, 67% coverage: 6:158/228 of query aligns to 8:162/240 of 1zud3

query
sites
1zud3

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active site: R11 (≠ K9), D127 (= D123)
binding calcium ion: L14 (vs. gap), D16 (≠ G12)

Sites not aligning to the query:

binding zinc ion: 169, 235, 238

1zfnA Structural analysis of escherichia coli thif (see paper)
27% identity, 67% coverage: 6:158/228 of query aligns to 8:162/244 of 1zfnA

query
sites
1zfnA

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active site: R11 (≠ K9), D127 (= D123)
binding adenosine-5'-triphosphate: I34 (≠ V30), G35 (= G31), G37 (= G33), G38 (= G34), D59 (= D55), R70 (= R66), Q71 (= Q67), K83 (= K79), T126 (= T122), D127 (= D123), T131 (≠ I127)

Sites not aligning to the query:

binding zinc ion: 169, 172, 240, 243

1jwbB Structure of the covalent acyl-adenylate form of the moeb-moad protein complex (see paper)
25% identity, 94% coverage: 11:224/228 of query aligns to 17:226/240 of 1jwbB

query
sites
1jwbB

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active site: D129 (= D123)
binding adenosine monophosphate: G37 (= G31), G39 (= G33), G40 (= G34), D61 (= D55), F62 (≠ M56), K85 (= K79), L108 (≠ K102), C127 (≠ A121), T128 (= T122), D129 (= D123), N130 (= N124), V133 (≠ I127)
binding zinc ion: C171 (vs. gap)

Sites not aligning to the query:

active site: 13
binding zinc ion: 236, 239

1jw9B Structure of the native moeb-moad protein complex (see paper)
25% identity, 94% coverage: 11:224/228 of query aligns to 17:226/240 of 1jw9B

query
sites
1jw9B

L

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active site: D129 (= D123)
binding zinc ion: C171 (vs. gap)

Sites not aligning to the query:

active site: 13
binding zinc ion: 236, 239

1jwaB Structure of the atp-bound moeb-moad protein complex (see paper)
24% identity, 94% coverage: 11:224/228 of query aligns to 17:211/217 of 1jwaB

query
sites
1jwaB

L

L

G

R

A

G

L

F

S

D

I

F

D

D

A

G

Q

Q

K

E

K

A

L

L

L

K

S

D

T

S

T

R

V

V

A

L

V

I

V

V

G

G

V

L

G
|
G

I

L

G

G

G

C

F

A

L

A

I

S

E

Q

G

Y

L

L

A

A

R

S

L

A

G

G

V

V

K

G

K

N

I

L

I

T

A

L

V

L

D
|
D

M
x
F

D

D

S

T

F

V

D

S

E

L

T

S

N

N

L

L

N

Q

R
|
R

Q

Q

I

T

I

L

S

H

N

S

V

D

N

A

N

T

L

V

G

G

K

Q

F

P

K
|
K

V

V

F

E

E

S

A

A

E

R

K

D

R

A

V

L

K

T

E

R

I

I

N

N

P

P

A

H

V

I

Y

A

F

I

E

T

P

P

I

V

K

N

E

A

K
x
L

A

L

Y

D

L

D

E

A

N

E

L

L

D

A

I

A

F

L

L

I

M

A

E

E

A

H

S

D

Y

L

I

V

F

L

D

D

A

C

T

T

D
|
D

N
|
N

I

V

E

A

I
x
V

R

R

K

N

S

Q

L

L

S

N

K

A

F

G

A

C

Q

F

N

A

M

A

G

K

K

V

I

P

L

L

I

V

H

S

G

G

G

A

C

A

A

I

G

R

W

M

Y

E

A

G

Q

Q

I

I

A

T

V

V

I

F

T

T

K

Y

D

E

T

A

P

G

G

V

I

M

E

A

K

P

L

L

L

I

G

G

E

-

T

-

N

-

V

-

E

-

G

-

A

-

E

-

K

-

D

-

M

-

G

-

N

-

P

-

I

-

F

-

A

-

P

-

M

-

L

V

T

I

A

G

A

S

L

L

E

Q

L

A

S

M

E

E

F

A

C

I

K

K

L

M

I

L

S

A

V

G

Q

Y

G

G

E

K

N

P

L

A

I

S

G

G

K

K

C

I

M

V

V

M

V

Y

N

D

L

A

L

M

T

T

N

C

E

Q

Y

F

R

R

active site: D129 (= D123)
binding adenosine-5'-triphosphate: G39 (= G33), G40 (= G34), D61 (= D55), F62 (≠ M56), R72 (= R66), K85 (= K79), L108 (≠ K102), D129 (= D123), N130 (= N124), V133 (≠ I127)

Sites not aligning to the query:

active site: 13

O59954 Adenylyltransferase and sulfurtransferase uba4; Common component for nitrate reductase and xanthine dehydrogenase protein F; Ubiquitin-like protein activator 4; EC 2.7.7.80; EC 2.8.1.11 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see paper)
35% identity, 61% coverage: 5:143/228 of query aligns to 67:207/482 of O59954

query
sites
O59954

K

R

R

R

Y

Y

L

G

K

R

N

Q

L

M

-

I

-

V

G

P

A

Q

L

F

S

G

I

L

D

Q

A
x
G

Q

Q

K

L

K

K

L

L

L

R

S

D

T

A

T

K

V

V

A

L

V

I

V

V

G

G

V

A

G

G

I

L

G
|
G

G

C

F

P

L

A

I

A

E

L

G

Y

L

L

A

A

R

G

L

A

G

G

V

V

K

G

K

T

I

I

I

G

A

L

V

V

D

D

M

G

D

D

S

T

F

V

D

E

E

A

T

S

N

N

L

L

N

H

R
|
R

Q

Q

I

V

I

L

S

H

N

R

V

S

N

R

N

N

L

V

G

G

K

K

F

L

K

K

V

V

F

D

E

S

A

A

E

I

K

E

R

Y

V

L

K

R

E

E

I

L

N

N

P

P

A

H

V

P

Y

T

F

Y

E

-

P

-

I

I

K

A

E

H

K

Q

A

A

Y

H

L

L

-

T

-

P

-

R

E

E

N

A

L

P

D

D

I

I

F

F

L

-

M

K

E

D

A

Y

S

D

Y

L

I

I

F

L

D

D

A
x
C

T

T

D

D

N

N

I

P

E

A

I

T

R

R

K

Y

S

L

L

I

S

S

K

D

F

T

A

A

Q

V

N

L

M

L

G

G

K

K

I

P

L

L

I

V

G82 (≠ A18) mutation to D: In cnxF21ts and cnxF24ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.
G100 (= G36) mutation to S: In cnxF1285; impairs molybdopterin biosynthesis.
R130 (= R66) mutation to Q: In cnxF200; impairs molybdopterin biosynthesis.
C185 (≠ A121) mutation to Y: In cnxF472; impairs molybdopterin biosynthesis.

Sites not aligning to the query:

215 E→K: In cnxF119; impairs molybdopterin biosynthesis.
264 G→S: In cnxF142ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.

4yedD Tcda (csdl) (see paper)
31% identity, 54% coverage: 27:149/228 of query aligns to 32:155/255 of 4yedD

query
sites
4yedD

V

I

A

C

V

V

G
|
G

V

I

G

G

G
|
G

I

V

G

G

G

S

F

W

L

A

I

A

E

E

G

A

L

L

A

A

R

R

L

T

G

G

V

I

K

G

K

A

I

I

I

T

A

L

V
x
I

D
|
D

M

M

D
|
D

S

D

F

V

D

C

E

V

T

T

N

N

L

T

N

N

R

R

Q

Q

I

I

I

H

S

A

N

L

V

R

N

D

N

N

L

V

G

G

K

L

F

A

K
|
K

V

A

F

E

E

V

A

M

E

A

K

E

R

R

V

I

K

R

E

Q

I

I

N

N

P

P

A

E

V

C

Y

R

F

V

E

T

P

V

I

V

K

D

E

D

K
x
F

A
x
V

Y

T

L

P

E

D

N

N

L

V

D

A

I

Q

F

Y

L

M

-

S

M

V

E

G

A

Y

S

S

Y

Y

I

V

F

I

D

D

A

A

T

I

D
|
D

N

S

I

V

E

R

I
x
P

R

K

K

A

S

A

L

L

S

I

K

A

F

Y

A

C

Q

R

N

R

M

N

G

K

K

I

I

P

L

L

I

V

H

T

G

T

G

G

C

G

A

A

G

G

binding adenosine monophosphate: G36 (= G31), G39 (= G34), I59 (≠ V54), D60 (= D55), D62 (= D57), K84 (= K79), F107 (≠ K102), V108 (≠ A103), D129 (= D123), P133 (≠ I127)

4d79A Crystal structure of e. Coli tRNA n6-threonylcarbamoyladenosine dehydratase, tcda, in complex with atp at 1.768 angstroem resolution (see paper)
31% identity, 54% coverage: 27:149/228 of query aligns to 31:154/243 of 4d79A

query
sites
4d79A

V

I

A

C

V

V

G
|
G

V

I

G
|
G

I

V

G

G

G

S

F

W

L

A

I

A

E

E

G

A

L

L

A

A

R

R

L

T

G

G

V

I

K

G

K

A

I

I

I

T

A

L

V

I

D
|
D

M

M

D

D

S

D

F

V

D

C

E

V

T

T

N

N

L

T

N

N

R
|
R

Q

Q

I

I

I

H

S

A

N

L

V

R

N

D

N

N

L

V

G

G

K

L

F

A

K
|
K

V

A

F

E

E

V

A

M

E

A

K

E

R

R

V

I

K

R

E

Q

I

I

N

N

P

P

A

E

V

C

Y

R

F

V

E

T

P

V

I

V

K

D

E

D

K
x
F

A
x
V

Y

T

L

P

E

D

N

N

L

V

D

A

I

Q

F

Y

L

M

-

S

M

V

E

G

A

Y

S

S

Y

Y

I

V

F

I

D

D

A

A

T

I

D
|
D

N

S

I

V

E

R

I
x
P

R

K

K

A

S

A

L

L

S

I

K

A

F

Y

A

C

Q

R

N

R

M

N

G

K

K

I

I

P

L

L

I

V

H

T

G

T

G

G

C

G

A

A

G

G

binding adenosine-5'-triphosphate: G35 (= G31), G37 (= G33), G38 (= G34), D59 (= D55), R70 (= R66), K83 (= K79), F106 (≠ K102), V107 (≠ A103), D128 (= D123), P132 (≠ I127)

O42939 Ubiquitin-activating enzyme E1-like; Pmt3-activating enzyme subunit 2 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
32% identity, 62% coverage: 16:157/228 of query aligns to 17:159/628 of O42939

query
sites
O42939

I

V

D

E

A

A

Q

L

K

R

K

N

L

F

L

K

S

S

T

A

T

K

V

V

A

L

V

L

V

V

G

G

V

A

G

G

I

I

G

G

G

C

F

E

L

L

I

L

E

K

G

N

L

L

A

L

R

M

L

S

G

G

V

V

K

K

K

E

I

V

I

H

A

I

V

I

D

D

M

L

D

D

S

T

F

I

D

D

E

L

T

S

N

N

L

L

N

N

R

R

Q

Q

I

F

I

L

S

F

N

R

V

K

N

K

N

H

L

V

G

K

K

Q

F

P

K

K

V

A

F

I

E

V

A

A

E

A

K

K

R

T

V

A

K

S

E

S

I

F

N

N

P

P

A

N

V

V

-

K

-

L

-

E

-

A

Y

Y

F

H

E

A

P

N

I

I

K

K

E

E

K

D

A

R

Y

F

L

-

E

-

N

N

L

V

D

A

I

W

F

F

L

-

M

R

E

Q

A

F

S

D

Y

L

I

V

F

F

D

N

A

A

T

L

D

D

N

N

I

L

E

D

I

A

R

R

K

R

S

H

L

V

S

N

K

K

F

Q

A

C

Q

L

N

L

M

A

G

S

K

V

I

P

L

L

I

I

H

E

G

S

G

G

C

T

A

T

G

G

W

F

Y

L

A

G

Q

Q

I

V

A

Q

V

V

I

I

Sites not aligning to the query:

559 modified: Phosphoserine
563 modified: Phosphoserine

Q7SXG4 SUMO-activating enzyme subunit 2; Ubiquitin-like 1-activating enzyme E1B; Ubiquitin-like modifier-activating enzyme 2; EC 2.3.2.- from Danio rerio (Zebrafish) (Brachydanio rerio) (see paper)
32% identity, 65% coverage: 11:159/228 of query aligns to 5:154/650 of Q7SXG4

query
sites
Q7SXG4

L

V

G

G

A

P

L

L

S

R

I

K

D

Q

A

L

Q

A

K

D

K

S

L

L

L

S

S

S

T

C

T

R

V

V

A

L

V

V

G

G

V

A

G

G

I

I

G

G

G

C

F

E

L

L

I

L

E

K

G

N

L

L

A

V

R

L

L

T

G

G

V

F

K

K

K

N

I

I

I

E

A

V

V

I

D

D

M

L

D

D

S

T

F

I

D

D

E

V

T

S

N

N

L

L

N

N

R

R

Q

Q

I

F

I

L

S

F

N

Q

V

K

N

K

N

H

L

V

G

G

K

K

F

S

K

K

V

A

F

Q

E

V

A

A

E

K

K

E

R

S

V

V

K

L

E

R

I

F

N

C

P

P

A

S

-

A

-

N

-

I

-

T

V

A

Y

Y

F

H

E

D

P

S

I

I

K

M

E

N

K

P

A

D

Y

Y

L

-

E

-

N

N

L

V

D

E

I

-

F

F

L

F

M

R

E

N

A

F

S

Q

Y

L

I

V

F

M

D

N

A

A

T

L

D

D

N

N

I

R

E

A

I

A

R

R

K

N

S

H

L

V

S

N

K

R

F

M

A

C

Q

L

N

A

M

A

G

D

K

I

I

P

L

L

I

I

H

E

G

S

G

G

C

T

A

A

G

G

W

Y

Y

L

A

G

Q

Q

I

V

A

T

V

V

I

I

T

K

K

K

Sites not aligning to the query:

599 modified: Phosphoserine

D4GSF3 SAMP-activating enzyme E1; Ubiquitin-like activating enzyme of archaea; Ubl-activating enzyme; EC 2.7.7.- from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) (see paper)
26% identity, 98% coverage: 5:228/228 of query aligns to 11:239/270 of D4GSF3

query
sites
D4GSF3

K

D

R

R

Y

Y

L

S

K

R

N

H

-

I

L

M

G

D

A

E

L

V

S

G

I

P

D

E

A

G

Q

Q

K

G

K

R

L

L

S

S

T

S

T

R

V

V

A

V

V

V

G

G

V

A

G

G

I

L

G

G

G

A

F

P

L

A

I

I

E

Q

G

Y

L

L

A

A

R

A

L

V

G

G

V

V

K

G

K

E

I

L

I

V

A

V

V

V

D

D

M

D

D

D

S

V

F

V

D

E

E

R

T

S

N

N

L

L

N

Q

R

R

Q

Q

I

V

S

H

N

C

V

D

N

D

L

V

G

G

K

T

F

P

K

K

V

A

F

E

E

S

A

A

E

A

K

A

R

F

V

V

K

R

E

G

I

L

N

N

P

P

A

D

V

V

Y

S

F

V

E

E

P

P

I

V

K

E

E

A

K

R

A

V

Y

D

L

K

E

S

N

N

L

V

D

H

I

E

F

V

L

V

M

A

E

G

A

S

S

D

Y

V

I

V

F

V

D

D

A

A

T

S

D

D

N

N

I

F

E

P

I

T

R

R

K

Y

S

L

L

L

S

N

K

D

F

V

A

C

Q

R

N

F

M

E

G

G

K

I

I

P

L

L

I

V

H

H

G

G

G

A

C

I

A

Y

G

K

W

F

Y

E

A

G

Q

Q

I

A

A

T

V

T

I

L

T

V

K

P

D

D

T

G

P

P

G

C

I

Y

E

R

K

C

L

L

L

F

G

P

E

E

T

A

N

P

V

E

E

P

G

G

A

T

E

V

K

P

D

D

M
x
C

G

A

N

T

P

T

-

G

-

V

-

L

I

G

F

V

A

L

P

P

M

G

L

T

T

V

A

G

A

C

L

I

E

Q

L

A

S

T

E

E

F

A

C

M

K

K

L

L

I

L

S

L

V

D

Q

E

G

G

E

E

N

A

L

L

I

D

G

G

K

R

C

L

M

L

V

F

V

Y

N

D

L

A

L

M

T

D

N

M

E

T

Y

F

R

E

V

T

F

V

E

P

F

Y

C188 (≠ M180) mutation to S: Loss of activity since this mutant is not able to complement a ubaA deletion in trans to restore sampylation and tRNA thiolation.

P38820 Adenylyltransferase and sulfurtransferase UBA4; Needs CLA4 to survive protein 3; Ubiquitin-like protein activator 4; EC 2.7.7.-; EC 2.8.1.- from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 4 papers)
31% identity, 62% coverage: 16:157/228 of query aligns to 59:200/440 of P38820

query
sites
P38820

I

V

D

A

A

G

Q

Q

K

V

K

K

L

L

L

K

S

N

T

T

T

K

V

V

A

L

V

V

G

G

V

A

G

G

I

L

G

G

G

C

F

P

L

A

I

L

E

P

G

Y

L

L

A

A

R

G

L

A

G

G

V

V

K

G

K

Q

I

I

I

G

A

I

V

V

D

D

M

N

D

D

S

V

F

V

D

E

E

T

T

S

N

N

L

L

N

H

R

R

Q

Q

I

V

I

L

S

H

N

D

V

S

N

S

N

R

L

V

G

G

K

M

F

L

K

K

V

C

F

E

E

S

A

A

E

R

K

Q

R

Y

V

I

K

T

E

K

I

L

N

N

P

P

A

H

-

I

-

N

V

V

Y

V

F

T

E

Y

P

P

I

V

K

R

E

L

K

N

A

S

Y

S

L

-

E

N

N

A

L

F

D

D

I

I

F

F

L

-

M

K

E

G

A

Y

S

N

Y

Y

I

I

F

L

D

D

A

C

T

T

D

D

N

S

I

P

E

L

I

T

R

R

K

Y

S

L

L

V

S

S

K

D

F

V

A

A

Q

V

N

N

M

L

G

G

K

I

I

T

L

V

I

V

H

S

G

A

G

S

C

G

A

L

G

G

W

T

Y

E

A

G

Q

Q

I

L

A

T

V

I

I

L

Sites not aligning to the query:

225 mutation C->A,S: Abolishes adenylyltransferase activity but not sulfurtransferase activity.
397 mutation C->A,S: Abolishes sulfurtransferase activity but not adenylyltransferase activity.

6yubB Crystal structure of uba4 from chaetomium thermophilum (see paper)
28% identity, 69% coverage: 5:162/228 of query aligns to 13:173/289 of 6yubB

query
sites
6yubB

K

E

R

R

Y

Y

L

S

K

R

N

Q

L

M

-

I

-

V

G

P

A

G

L

M

S

G

I

K

D

E

A

G

Q

Q

K

L

K

R

L

L

L

M

S

N

T

A

T

K

V

V

A

L

V

I

G

G

V

A

G

G

I

L

G

G

G

C

F

P

L

A

I

A

E

Q

G

Y

L

L

A

A

R

G

L

A

G

G

V

V

K

G

K

T

I

I

I

G

A

I

V

V

D

D

M

G

D

D

S

S

F

V

D

E

E

T

T

S

N

N

L

L

N

H

R

R

Q

Q

I

V

I

A

S

H

N

A

V

T

N

K

N

R

L

V

G

G

K

M

F

L

K

K

V

V

F

D

E

S

A

L

E

I

K

T

R

H

V

L

K

I

E

E

I

I

N

N

P

P

A

L

V

P

Y

V

F

Y

E

V

P

P

I

Y

K

R

E

F

K

D

A

L

Y

T

L

P

E

Q

N

N

L

A

D

A

I

Q

F

I

L

I

M

K

E

P

A

W

S

D

Y

V

I

I

F

L

D

D

A

C

T

T

D

D

N

N

I

P

E

A

I

T

R

R

K

Y

S

L

L

I

S

S

K

D

F

V

A

C

Q

V

N

L

M

L

G

G

K

K

I

P

L

L

I

V

H

S

G

A

C

S

A

V

G

Q

W

K

Y

S

A

G

Q

Q

I

L

A

I

V

V

I

L

-

N

T

C

K

P

D

P

T

T

P

P

Sites not aligning to the query:

binding zinc ion: 184, 187, 265

Q72J02 Sulfur carrier protein adenylyltransferase; E1-like protein TtuC; Sulfur carrier protein MoaD adenylyltransferase; Sulfur carrier protein ThiS adenylyltransferase; Sulfur carrier protein TtuB adenylyltransferase; tRNA two-thiouridine-synthesizing protein C; EC 2.7.7.80; EC 2.7.7.73; EC 2.7.7.- from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see paper)
26% identity, 78% coverage: 47:224/228 of query aligns to 53:239/271 of Q72J02

query
sites
Q72J02

G

G

V

V

K

G

K

R

I

V

I

G

A

V

V

V

D

E

M

M

D

D

S

R

F

V

D

E

E

V

T

S

N

N

L

L

N

H

R

R

Q

Q

I

V

I

L

S

Y

N

T

V

T

N

E

N

D

L

V

G

G

K

E

F

P

K

K

V

A

F

L

E

V

A

A

E

Q

K

K

R

R

V

L

K

Q

E

A

I

L

N

N

P

P

A

L

V

V

Y

R

F

V

E

E

P

A

I

Y

K

P

E

V

K

R

A

L

Y

T

L

S

E

E

N

N

L

A

D

L

I

E

F

I

L

L

M

R

E

P

A

Y

S

D

Y

L

I

V

F

V

D

D

A

A

T

S

D

D

N

N

I

F

E

P

I

T

R

R

K

Y

S

L

L

V

S

N

K

D

F

A

A

A

Q

V

N

L

M

L

G

G

K

K

I

P

L

L

I

V

H

F

G

G

G

A

C

I

A

Y

G

Q

W

F

Y

D

A

G

Q

Q

I

V

A

A

V

V

I

F

T

H

K

H

D

P

T

T

-

L

-

H

-

G

-

E

-

M

-

G

P

P

G

C

I

Y

E

R

K

C

L

L

L

F

G

P

E

K

T

P

N

P

V

P

E

P

G

G

A

A

E

V

K

P

D

S

M
x
C

G

A

N

E

P

A

-

G

I

V

F

F

A

G

-

V

-

L

P

P

M

A

L

V

T

V

A

G

A

S

L

L

E

M

L

A

S

A

E

E

F

A

C

L

K

K

L

V

I

L

S

L

V

G

Q

I

G

G

E

K

N

P

L

L

I

A

G

G

K

H

C

L

M

L

V

L

V

Y

N

D

L

A

L

L

T

E

N

A

E

S

Y

F

R

R

C192 (≠ M180) modified: Glycyl cysteine thioester (Cys-Gly) (interchain with G-Cter in TtuB); mutation to S: Not able to form a thioester complex with TtuB.

Sites not aligning to the query:

268 C→S: Still able to form a thioester complex with TtuB.

6yubA Crystal structure of uba4 from chaetomium thermophilum (see paper)
28% identity, 67% coverage: 5:157/228 of query aligns to 12:166/423 of 6yubA

query
sites
6yubA

K

E

R

R

Y

Y

L

S

K

R

N

Q

L

M

-

I

-

V

G

P

A

G

L

M

S

G

I

K

D

E

A

G

Q

Q

K

L

K

R

L

L

L

M

S

N

T

A

T

K

V

V

A

L

V

I

G

G

V

A

G

G

I

L

G

G

G

C

F

P

L

A

I

A

E

Q

G

Y

L

L

A

A

R

G

L

A

G

G

V

V

K

G

K

T

I

I

I

G

A

I

V

V

D

D

M

G

D

D

S

S

F

V

D

E

E

T

T

S

N

N

L

L

N

H

R

R

Q

Q

I

V

I

A

S

H

N

A

V

T

N

K

N

R

L

V

G

G

K

M

F

L

K

K

V

V

F

D

E

S

A

L

E

I

K

T

R

H

V

L

K

I

E

E

I

I

N

N

P

P

A

L

V

P

Y

V

F

Y

E

V

P

P

I

Y

K

R

E

F

K

D

A

L

Y

T

L

P

E

Q

N

N

L

A

D

A

I

Q

F

I

L

I

M

K

E

P

A

W

S

D

Y

V

I

I

F

L

D

D

A

C

T

T

D

D

N

N

I

P

E

A

I

T

R

R

K

Y

S

L

L

I

S

S

K

D

F

V

A

C

Q

V

N

L

M

L

G

G

K

K

I

P

L

L

I

V

H

S

G

A

C

S

A

V

G

Q

W

K

Y

S

A

G

Q

Q

I

L

A

I

V

V

I

L

Sites not aligning to the query:

binding zinc ion: 183, 186, 266, 269

Q9VLJ8 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Ubiquitin activating enzyme 4; EC 2.7.7.80; EC 2.8.1.11 from Drosophila melanogaster (Fruit fly) (see paper)
27% identity, 66% coverage: 6:156/228 of query aligns to 71:223/453 of Q9VLJ8

query
sites
Q9VLJ8

R

R

Y

Y

L

S

K

R

N

Q

L

L

-

I

-

L

G

P

A

D

L

F

S

G

I

V

D

Q

A

G

Q

Q

K

L

K

K

L

L

L

K

S

N

T

S

V

V

A

L

V

I

V

V

G

G

V

L

G

G

I

L

G

G

G

C

F

P

L

A

I

A

E

Q

G

Y

L

L

A

A

R

A

L

A

G

G

V

C

K

G

K

H

I

L

I

G

A

L

V

V

D

D

M

Y

D

D

S

E

F

V

D

E

E

R

T

S

N

N

L

F

N

H

R

R

Q

Q

I

I

I

L

S

H

N

S

V

E

N

D

N

R

L

C

G

G

K

M

F

S

K

K

V

A

F

E

E

S

A

A

E

R

K

I

R

A

V

L

K

L

E

E

I

L

N

N

P

P

A

H

V

C

Y

E

F

I

E

Q

P

C

I

H

K

S

E

R

K

M

A

L

Y

Y

L

P

E

H

N

N

L

A

D

M

I

H

F

I

L

I

M

R

E

G

A

Y

S

D

Y

V

I

V

F

L

D

D

A

C

T

T

D

D

N

N

I

V

E

P

I

T

R

R

K

Y

S

L

L

L

S

S

K

D

F

A

A

C

Q

V

N

M

M

L

G

S

K

K

I

P

L

L

I

V

H

S

G

G

G

S

C

A

A

L

G

K

W

M

Y

D

A

G

Q

Q

I

L

A

T

V

V

Sites not aligning to the query:

62 modified: Phosphothreonine

5tr4A Structure of ubiquitin activating enzyme (uba1) in complex with ubiquitin and tak-243
27% identity, 69% coverage: 1:157/228 of query aligns to 398:565/972 of 5tr4A

query
sites
5tr4A

M

Q

A

P

V

V

Q

N

K

S

R

R

Y

Y

L

D

K

N

N

Q

L

I

G

A

A

V

L

F

S

G

I

L

D

D

A

F

Q

Q

K

K

L

I

L

A

S

N

T

S

T

K

V

V

A

F

V

L

V

V

G

G

V

S

G

G

G
x
A

I

I

G

G

G

C

F

E

L

M

I

L

E

K

G

N

L

W

A

A

R

L

L

L

G

G

V

L

K

G

K

S

-

G

-

S

-

D

-

G

-

Y

I

I

I

V

A

V

V

T

D
|
D

M
|
M

D

D

S

S

F

I

D

E

E

K

T

S

N

N

L

L

N

N

R
|
R

Q

Q

I

F

I

L

S

F

N

R

V

P

N

K

N

D

L

V

G

G

K

K

F

N

K
|
K

V

S

F

E

E

V

A

A

E

A

K

E

R

A

V

V

K

C

E

A

I

M

N

N

P

P

A

D

V

L

Y

K

-

G

-

K

-

I

-

N

-

A

-

K

-

I

-

D

-
x
R

-
x
V

-

G

-

P

-

E

F

T

E

E

P

E

I

I

K

F

E

N

K

D

A

S

Y

F

L

W

E

E

N

S

L

L

D

D

I

-

F

-

L

-

M

-

E

-

A

-

S

-

Y

F

I

V

F

T

D

N

A
|
A

T
x
L

D
|
D

N
|
N

I

V

E
x
D

I
x
A

R

R

K

T

S
x
Y

L

V

S

D

K

R

F

R

A

C

Q

V

N

F

M

Y

G

R

K

K

I

P

L

L

I

L

H

E

G

S

G

G

C

T

A

L

G

G

W

T

Y

K

A

G

Q

N

I

T

A

Q

V

V

I

I

active site: R468 (= R66), D531 (= D123)
binding [(1~{R},2~{R},3~{S},4~{R})-2,3-bis(oxidanyl)-4-[[2-[3-(trifluoromethylsulfanyl)phenyl]pyrazolo[1,5-a]pyrimidin-7-yl]amino]cyclopentyl]methyl sulfamate: A431 (≠ G34), D457 (= D55), M458 (= M56), R468 (= R66), K481 (= K79), R506 (vs. gap), V507 (vs. gap), A529 (= A121), L530 (≠ T122), N532 (= N124), D534 (≠ E126), A535 (≠ I127), Y538 (≠ S130)

Sites not aligning to the query:

active site: 8, 587, 588, 590, 751, 752

6dc6C Crystal structure of human ubiquitin activating enzyme e1 (uba1) in complex with ubiquitin (see paper)
27% identity, 68% coverage: 4:157/228 of query aligns to 400:562/996 of 6dc6C

query
sites
6dc6C

Q

Q

K

N

R

R

Y

Y

L

D

K

G

N

Q

L

V

G

A

A

V

L

F

S

G

I

S

D

D

A

L

Q

Q

K

E

K

K

L

L

L

G

S

K

T

Q

T

K

V

Y

A

F

V

L

V

V

G

G

V

A

G

G

G

A

I

I

G

G

G

C

F

E

L

L

I

L

E

K

G

N

L

F

A

A

R

M

L

I

G

G

V

L

K

G

-

C

-

G

-

E

-

G

K

E

I

I

A

V

V

T

D

D

M

M

D
|
D

S

T

F

I

D
x
E

E

K

T

S

N
|
N

L

L

N

N

R
|
R

Q

Q

I

F

I

L

S

F

N

R

V

P

N

W

N

D

L

V

G

T

K

K

F

L

K
|
K

V

S

F

D

E

T

A

A

E

A

K

A

R

A

V

V

K

R

E

Q

I

M

N

N

P

P

A

H

V

I

Y

R

F

V

E

T

-

S

-

H

-

Q

-

N

-

R

-

V

-

G

P

P

I

D

K

T

E

E

K

R

A

I

Y

Y

L

-

E

-

N

-

L

D

D

D

I

D

F

F

L

F

M

Q

E

N

A

L

S

D

Y

G

I

V

F

A

D

N

A

A

T

L

D
|
D

N

N

I

V

E

D

I

A

R

R

K

M

S

Y

L

M

S

D

K

R

F

R

A

C

Q

V

N

Y

M

Y

G

R

K

K

I

P

L

L

I

L

H

E

G

S

G

G

C

T

A

L

G

G

W

T

Y

K

A

G

Q

N

I

V

A

Q

V

V

I

V

active site: R467 (= R66), D528 (= D123)
binding magnesium ion: D458 (= D57), E461 (≠ D60)
binding pyrophosphate 2-: N464 (= N63), K480 (= K79)

Sites not aligning to the query:

active site: 9, 584, 585, 587
binding pyrophosphate 2-: 9

Query Sequence

>WP_013430763.1 NCBI__GCF_000166775.1:WP_013430763.1
MAVQKRYLKNLGALSIDAQKKLLSTTVAVVGVGGIGGFLIEGLARLGVKKIIAVDMDSFD
ETNLNRQIISNVNNLGKFKVFEAEKRVKEINPAVYFEPIKEKAYLENLDIFLMEASYIFD
ATDNIEIRKSLSKFAQNMGKILIHGGCAGWYAQIAVITKDTPGIEKLLGETNVEGAEKDM
GNPIFAPMLTAALELSEFCKLISVQGENLIGKCMVVNLLTNEYRVFEF

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory