SitesBLAST

Q8ZPZ9 N-acetyl-D-glucosamine kinase; GlcNAc kinase; EC 2.7.1.59 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
30% identity, 99% coverage: 1:312/316 of query aligns to 1:299/303 of Q8ZPZ9

query
sites
Q8ZPZ9

M

M

Y

Y

I

-

G

G

I

F

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T

T

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-

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E

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L

K

D

D

A

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V

A

C

E

E

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L

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-

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-

N

-

L

-

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E

E

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C

A

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D

L

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D

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A

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N

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E

K

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Y

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D

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N

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D

A

A

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C

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A

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Y

Y

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D

Y

L

L

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A

A

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C

L

L

A

G

N

N

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N

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N

E

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Y

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N

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L

H157 (= H163) binding Zn(2+)
C177 (= C173) binding Zn(2+)
C179 (= C175) binding Zn(2+)
C184 (= C180) binding Zn(2+)

2ap1A Crystal structure of the putative regulatory protein
30% identity, 99% coverage: 1:312/316 of query aligns to 3:301/305 of 2ap1A

query
sites
2ap1A

M

M

Y

Y

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-

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G

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I

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M

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D

D

A

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C

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E

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-

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E

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binding zinc ion: H159 (= H163), C179 (= C173), C181 (= C175), C186 (= C180)

2yhwA High-resolution crystal structures of n-acetylmannosamine kinase: insights about substrate specificity, activity and inhibitor modelling. (see paper)
31% identity, 87% coverage: 4:277/316 of query aligns to 6:274/309 of 2yhwA

query
sites
2yhwA

I

L

G

A

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D

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K

T

Y

A

L

L

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E

L

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N

N

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binding calcium ion: N112 (= N110), N115 (= N113), G144 (= G142), A161 (= A159)
binding zinc ion: H165 (= H163), C175 (= C173), C177 (= C175), C182 (= C180)

6jdbA Crystal structure of n-acetyl mannosmaine kinase in complex with mannac-6p and adp from haemophilus influenzae
29% identity, 100% coverage: 1:316/316 of query aligns to 1:288/290 of 6jdbA

query
sites
6jdbA

M

M

Y

R

Y

C

I

L

G

A

I

L

D

D

L

I

G

G

T

T

N
x
K

I

I

A

A

G

A

I

I

V

V

-

K

-

N

-

E

-

I

D

E

E

Q

G

R

G

Q

K

Q

I

I

-

H

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L

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A

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D

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-

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N

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x
T

G
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G

L

I

T

I

L

N

D

N

D

-

I

-

H

-

S

-

V

-

G

G

I

I

G

L

S

S

P
x
A

G
x
L

A
x
N

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P

D

K

N
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N

E

L

K

G

G

G

M

L

I

A

L

E

Y

F

S

-

N

-

I

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A

-

F

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L

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N

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P

P

M

L

R

K

E

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Y

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D

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P

P

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L

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D
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D

A

A

N

Q

C

A

A

A

A

T

Y

Y

G

A

E

E

Y

Y

I

Q

A

L

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Q

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N

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F

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E

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x
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I

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N

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C

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E

A

A

Y

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L

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x
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T

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K

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N

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A

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Y

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x
S

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E

A

G

E

E

G

Y

Y

L

-

L

L

E

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P

L

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E

K

K

L

Y

V

L

-

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D

Y

F

E

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S

F

I

Y

Y

C

C

K

C

Q

E

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P

E

M

T

P

A

K

K

I

-

P

-

A

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L

F

G

G

N

Q

D

D

A

A

G

G

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I

I

G

G

A

A

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Y

L

W

A

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K

Q

D

L

V

binding adenosine-5'-diphosphate: K12 (≠ N12), S129 (≠ G137), T130 (= T138), P195 (≠ A219), K196 (= K220), S241 (≠ G265)
binding 2-acetamido-2-deoxy-6-O-phosphono-alpha-D-mannopyranose: T62 (≠ C56), G63 (= G57), A72 (≠ P71), L73 (≠ G72), N74 (≠ A73), N77 (= N76), N102 (= N110), D103 (= D111), S129 (≠ G137), T130 (= T138), H152 (≠ E160), H155 (= H163), E174 (= E182)
binding zinc ion: H155 (= H163), C165 (= C173), C167 (= C175), C172 (= C180)

Q9Y223 Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; UDP-GlcNAc-2-epimerase/ManAc kinase; EC 3.2.1.183; EC 2.7.1.60 from Homo sapiens (Human) (see 26 papers)
31% identity, 87% coverage: 4:277/316 of query aligns to 410:682/722 of Q9Y223

query
sites
Q9Y223

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D413 (= D7) binding Mg(2+); to Y: in THC12; likely pathogenic; dbSNP:rs1280775456
G416 (= G10) binding an N-acyl-D-mannosamine 6-phosphate
T417 (= T11) binding ADP; to M: in THC12; likely pathogenic; dbSNP:rs1554659711
N418 (= N12) binding ADP
R420 (≠ A14) binding ADP; to Q: in THC12; likely pathogenic; dbSNP:rs780092539
I472 (= I68) to T: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; decreased N-acylmannosamine kinase activity corresponding to less than 10% of wild-type activity
G475 (≠ P71) to F: in THC12; likely pathogenic; requires 2 nucleotide substitutions
G476 (= G72) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate
R477 (≠ A73) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate
V485 (≠ I81) to R: in THC12; uncertain significance; reduced protein abundance in homozygous patient cells; requires 2 nucleotide substitutions
L486 (= L82) to P: in THC12; uncertain significance; dbSNP:rs774867424
T489 (≠ N85) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate
N516 (= N110) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate
D517 (= D111) active site; binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate; mutation D->A,N: Loss of N-acylmannosamine kinase activity. Decreased affinity for N-acyl-D-mannosamine. No effect on structure.
N519 (= N113) to S: in NM and THC12; likely pathogenic; decreased UDP-N-acetylglucosamine 2-epimerase activity; decreased N-acylmannosamine kinase activity; dbSNP:rs1554658910
A524 (≠ G118) to V: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity corresponding to less than 10% of wild-type activity; decreased N-acylmannosamine kinase activity; impaired homohexamers formation; dbSNP:rs764698870
F528 (≠ A122) to C: in NM; decreased N-acylmannosamine kinase activity; dbSNP:rs986773986
T544 (= T138) to R: in THC12; uncertain significance; reduced protein abundance in homozygous patient cells
G545 (= G139) binding an N-acyl-D-mannosamine 6-phosphate
C563 (≠ A157) to Y: in THC12; likely pathogenic; results in severely decreased cell surface sialylation
E566 (= E160) binding an N-acyl-D-mannosamine
H569 (= H163) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate; binding Zn(2+)
V572 (≠ I166) to L: in NM and THC12; likely pathogenic; mildly decreased UDP-N-acetylglucosamine 2-epimerase activity; decreased N-acylmannosamine kinase activity corresponding to less than 10% of wild-type activity; does not affect homohexamers formation; dbSNP:rs121908632
G576 (= G170) to E: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; decreased N-acylmannosamine kinase activity; dbSNP:rs121908625
C579 (= C173) binding Zn(2+)
C581 (= C175) binding Zn(2+)
C586 (= C180) binding Zn(2+)
I587 (≠ W181) to T: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; decreased N-acylmannosamine kinase activity; dbSNP:rs748949603
E588 (= E182) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate
A630 (= A225) to T: in NM; decreased N-acylmannosamine kinase activity; does not affect homohexamers formation; dbSNP:rs1382191649
A631 (≠ K226) to V: in NM; does not affect homohexamers formation; dbSNP:rs62541771

Sites not aligning to the query:

13 C → S: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; no effect on N-acylmannosamine kinase activity; dbSNP:rs1209266607
19 binding UDP
23 binding UDP
113 binding UDP
132 H → Q: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; impaired homohexamers formation
157 H → Y: in THC12; likely pathogenic
176 D → V: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; no effect on N-acylmannosamine kinase activity; impaired homohexamers formation; dbSNP:rs139425890
177 R → C: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; impaired homohexamers formation; dbSNP:rs539332585
200 I → F: in NM; uncertain significance; dbSNP:rs369328625
206 G → S: in NM; moderate phenotype with unusual involvement of quadriceps; dbSNP:rs766266918
220 binding UDP
253 binding UDP
259 binding CMP-N-acetyl-beta-neuraminate
263 R → L: in SIALURIA; strong reduction of feedback inhibition by CMP-Neu5Ac; dbSNP:rs121908623
266 R → Q: in SIALURIA; abolishes feedback inhibition by CMP-Neu5Ac; dbSNP:rs121908622; R → W: in sialuria; dbSNP:rs121908621
271 binding CMP-N-acetyl-beta-neuraminate
280 binding CMP-N-acetyl-beta-neuraminate
281 binding CMP-N-acetyl-beta-neuraminate
282 binding UDP
301 binding UDP
302 binding UDP
303 C → V: in NM; requires 2 nucleotide substitutions; dbSNP:rs121908633
307 binding UDP
321 binding UDP
331 V → A: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; no effect on N-acylmannosamine kinase activity; impaired homohexamers formation
378 D → Y: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; impaired homohexamers formation; dbSNP:rs199877522
420:722 natural variant: Missing (in THC12; likely pathogenic)
704 P → R: in THC12; likely pathogenic; the orthologous mutation in mouse embryos results in cerebrospinal hemorrhages and defective angiogenesis; results in loss of cell surface sialylation
708 G → S: in NM and THC12; likely pathogenic; decreased UDP-N-acetylglucosamine 2-epimerase activity; severely decreased N-acylmannosamine kinase activity; dbSNP:rs1554657922
712 M → T: in NM; decreased N-acylmannosamine kinase activity; dbSNP:rs28937594

Q91WG8 Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; UDP-GlcNAc-2-epimerase/ManAc kinase; EC 3.2.1.183; EC 2.7.1.60 from Mus musculus (Mouse) (see paper)
32% identity, 87% coverage: 4:277/316 of query aligns to 410:682/722 of Q91WG8

query
sites
Q91WG8

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C563 (≠ A157) mutation to Y: Loss-of-function mutant resulting in impaired sialic acid biosynthesis.

Sites not aligning to the query:

704 P→R: Homozygous embryos exhibit cerebrospinal hemorrhages and defective angiogenesis in the diencephalon at 11 dpc, and become non-viable between 11.5 and 12.5 dpc. Loss-of-function mutant resulting in impaired sialic acid biosynthesis.

O35826 Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; UDP-GlcNAc-2-epimerase/ManAc kinase; EC 3.2.1.183; EC 2.7.1.60 from Rattus norvegicus (Rat) (see paper)
31% identity, 89% coverage: 4:284/316 of query aligns to 410:686/722 of O35826

query
sites
O35826

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R
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R

M

-

T

-

R

-

E

-

A

-

A
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A
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K

D
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K

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L

N
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H

G
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M
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K
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L

L
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L

V
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N
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G
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G

-
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M

D
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S

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S
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P

K
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K

I
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D

D
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K
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G

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A

F
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L

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K
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M
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G

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K

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D
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K
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L
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L

A
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G
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G

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N
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N

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C
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L

N
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H

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F
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M

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N

P
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P

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C
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I
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L

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G
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G
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K

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K
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Q

D413 (= D7) mutation D->K,N: No effect on UDP-N-acetylglucosamine 2-epimerase activity. Does not affect feedback inhibition by CMP-Neu5Ac. Loss of N-acylmannosamine kinase activity. Does not interfere with oligomerization.
R420 (≠ A14) mutation to M: No effect on UDP-N-acetylglucosamine 2-epimerase activity. Does not affect feedback inhibition by CMP-Neu5Ac. Loss of N-acylmannosamine kinase activity. Does not interfere with oligomerization.

Sites not aligning to the query:

1 UDP-N-acetylglucosamine 2-epimerase
49 H→A: Loss UDP-N-acetylglucosamine 2-epimerase activity. No effect on N-acylmannosamine kinase activity. Does not interfere with enzyme oligomerization.
110 H→A: Loss UDP-N-acetylglucosamine 2-epimerase activity. No effect on N-acylmannosamine kinase activity. Partial reduction of the dimerization process.
132 H→A: Loss UDP-N-acetylglucosamine 2-epimerase activity. No effect on N-acylmannosamine kinase activity. Partial reduction of the dimerization process.
155 H→A: Loss UDP-N-acetylglucosamine 2-epimerase activity. No effect on N-acylmannosamine kinase activity. Strong reduction of the dimerization process.
157 H→A: Loss UDP-N-acetylglucosamine 2-epimerase activity. No effect on N-acylmannosamine kinase activity. Strong reduction of the dimerization process.
406:722 N-acetylmannosamine kinase

Query Sequence

>WP_013430999.1 NCBI__GCF_000166775.1:WP_013430999.1
MYYIGIDLGGTNIAAGIVDEGGKIIKKGSVPTGAHRHYTEIMKDMAELSLNLVKECGLTL
DDIHSVGIGSPGAPDNEKGMILYSNNIAFLNVPMREEIQKYIPKPVNIENDANCAAYGEY
IAGGAKGTKISVTITLGTGIGGGIIIDGKIFTGAHHAGAELGHMVICVDGEQCTCGRRGC
WEAYASATALIRMTREAAARDINGTIMKLVNGDISKIDAKTAFDAKRMGDSTGAAIVDRY
VKYLAEGLANICNIFEPEVICIGGGVSKEGEYLLEPVRKLVYEKFYCKQVPMPKIIPAVL
GNDAGIIGAALLAKQL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory