SitesBLAST
Comparing WP_013431307.1 NCBI__GCF_000166775.1:WP_013431307.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
2f02A Crystal structure of lacc from enterococcus faecalis in complex with atp
31% identity, 91% coverage: 1:286/315 of query aligns to 2:289/319 of 2f02A
- binding adenosine-5'-triphosphate: N186 (= N185), S224 (= S221), G226 (= G223), I243 (≠ A240), I246 (≠ A243), G253 (= G250), S254 (≠ A251), G255 (= G252), T258 (≠ M255), M280 (≠ L277), G283 (≠ A280), M284 (≠ A281)
3julA Crystal structure of listeria innocua d-tagatose-6-phosphate kinase bound with substrate
34% identity, 91% coverage: 1:287/315 of query aligns to 2:279/298 of 3julA
3ie7A The crystal structure of phosphofructokinase (lin2199) from listeria innocua in complex with atp at 1.6a
34% identity, 91% coverage: 1:287/315 of query aligns to 2:286/309 of 3ie7A
- binding adenosine-5'-triphosphate: N188 (= N185), S220 (= S221), G222 (= G223), A223 (= A224), G225 (= G226), V242 (≠ A243), G249 (= G250), A250 (= A251), G251 (= G252), D252 (= D253), S279 (≠ A280), V283 (= V284)
2jg1A Structure of staphylococcus aureus d-tagatose-6-phosphate kinase with cofactor and substrate (see paper)
32% identity, 88% coverage: 1:276/315 of query aligns to 9:285/318 of 2jg1A
- active site: G259 (= G250), S260 (≠ A251), G261 (= G252), D262 (= D253)
- binding phosphoaminophosphonic acid-adenylate ester: K46 (= K38), N193 (= N185), S230 (= S221), G232 (= G223), G235 (= G226), I252 (≠ A243), V254 (= V245), G259 (= G250), S260 (≠ A251), G261 (= G252), D262 (= D253), T264 (≠ M255)
Sites not aligning to the query:
2jgvB Structure of staphylococcus aureus d-tagatose-6-phosphate kinase in complex with adp (see paper)
32% identity, 88% coverage: 1:276/315 of query aligns to 5:281/314 of 2jgvB
Sites not aligning to the query:
2jg1C Structure of staphylococcus aureus d-tagatose-6-phosphate kinase with cofactor and substrate (see paper)
32% identity, 88% coverage: 1:276/315 of query aligns to 6:282/315 of 2jg1C
- active site: G256 (= G250), S257 (≠ A251), G258 (= G252), D259 (= D253)
- binding phosphoaminophosphonic acid-adenylate ester: S227 (= S221), G229 (= G223), A230 (= A224), G232 (= G226), I246 (≠ A240), I249 (≠ A243), V251 (= V245), V255 (≠ T249), G256 (= G250), S257 (≠ A251), G258 (= G252), D259 (= D253), T261 (≠ M255)
- binding 6-O-phosphono-beta-D-tagatofuranose: D17 (= D12), G42 (= G37), K43 (= K38), R93 (= R88), C95 (≠ N90), L108 (≠ N105), G140 (= G137), S141 (= S138), D259 (= D253)
Sites not aligning to the query:
2ajrA Crystal structure of possible 1-phosphofructokinase (ec 2.7.1.56) (tm0828) from thermotoga maritima at 2.46 a resolution
29% identity, 98% coverage: 1:309/315 of query aligns to 2:319/320 of 2ajrA
P9WID3 ATP-dependent 6-phosphofructokinase isozyme 2; ATP-PFK 2; Phosphofructokinase 2; Phosphofructokinase B; Phosphohexokinase 2; Tagatose-6-phosphate kinase; EC 2.7.1.11; EC 2.7.1.144 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
29% identity, 96% coverage: 2:302/315 of query aligns to 14:314/339 of P9WID3
- K283 (≠ S271) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
3uqdB Crystal structure of the phosphofructokinase-2 from escherichia coli in complex with substrates and products (see paper)
27% identity, 92% coverage: 2:290/315 of query aligns to 4:293/309 of 3uqdB
- active site: G253 (= G250), A254 (= A251), G255 (= G252), D256 (= D253)
- binding adenosine-5'-diphosphate: K185 (= K183), N187 (= N185), S224 (= S221), G226 (= G223), P227 (≠ A224), G229 (= G226), S248 (≠ V245), M258 (= M255), V280 (≠ L277), G283 (≠ A280), S284 (≠ A281)
- binding adenosine-5'-triphosphate: Y23 (≠ K21), K27 (≠ V25)
- binding 6-O-phosphono-beta-D-fructofuranose: K27 (≠ V25), R29 (= R27)
- binding 1,6-di-O-phosphono-beta-D-fructofuranose: D14 (= D12), G39 (= G37), N43 (= N41), R90 (= R88), R105 (≠ D103), S139 (= S138), G253 (= G250)
3uqdA Crystal structure of the phosphofructokinase-2 from escherichia coli in complex with substrates and products (see paper)
27% identity, 92% coverage: 2:290/315 of query aligns to 4:293/309 of 3uqdA
- active site: G253 (= G250), A254 (= A251), G255 (= G252), D256 (= D253)
- binding adenosine-5'-triphosphate: K185 (= K183), S224 (= S221), G226 (= G223), P227 (≠ A224), G229 (= G226), T251 (= T248), G255 (= G252), M258 (= M255), V280 (≠ L277), G283 (≠ A280), S284 (≠ A281), T287 (≠ V284)
- binding 6-O-phosphono-beta-D-fructofuranose: D14 (= D12), G38 (= G36), G39 (= G37), N43 (= N41), R90 (= R88), S139 (= S138), D256 (= D253)
- binding 1,6-di-O-phosphono-beta-D-fructofuranose: K27 (≠ V25), R29 (= R27)
3n1cA Crystal structure of the phosphofructokinase-2 from escherichia coli in complex with fructose-6-phosphate (see paper)
27% identity, 92% coverage: 2:290/315 of query aligns to 4:293/309 of 3n1cA
- active site: G253 (= G250), A254 (= A251), G255 (= G252), D256 (= D253)
- binding 6-O-phosphono-beta-D-fructofuranose: D14 (= D12), K27 (≠ V25), R29 (= R27), G39 (= G37), N43 (= N41), R90 (= R88), G138 (= G137), S139 (= S138), D256 (= D253)
P06999 ATP-dependent 6-phosphofructokinase isozyme 2; ATP-PFK 2; Phosphofructokinase 2; 6-phosphofructokinase isozyme II; Phosphohexokinase 2; EC 2.7.1.11 from Escherichia coli (strain K12) (see 3 papers)
27% identity, 92% coverage: 2:290/315 of query aligns to 4:293/309 of P06999
- K27 (≠ V25) binding ATP; binding ATP
- KPN 185:187 (= KPN 183:185) binding in other chain
- NQK 187:189 (≠ NIH 185:187) binding in other chain
- E190 (= E188) mutation to Q: Causes a 50-fold decrease in the kcat value and a 15-fold increment in the apparent KM for ATP.
- SLGPQG 224:229 (≠ SMGAKG 221:226) binding in other chain
- S248 (≠ V245) binding in other chain
- S250 (= S247) binding K(+)
- V252 (≠ T249) binding K(+)
- V280 (≠ L277) binding in other chain
- S284 (≠ A281) binding in other chain
- A286 (≠ K283) binding K(+)
- N289 (≠ K286) binding K(+)
- G291 (= G288) binding K(+)
- R293 (≠ K290) binding K(+)
3uqeA Crystal structure of the phosphofructokinase-2 mutant y23d from escherichia coli
28% identity, 89% coverage: 2:281/315 of query aligns to 4:286/307 of 3uqeA
- active site: G253 (= G250), A254 (= A251), G255 (= G252), D256 (= D253)
- binding adenosine-5'-triphosphate: K185 (= K183), N187 (= N185), S224 (= S221), G226 (= G223), P227 (≠ A224), G229 (= G226), S248 (≠ V245), A254 (= A251), G255 (= G252), M258 (= M255), V280 (≠ L275), G283 (≠ A278), S284 (≠ C279)
- binding pyrophosphate 2-: N187 (= N185), K189 (≠ H187)
Sites not aligning to the query:
3cqdA Structure of the tetrameric inhibited form of phosphofructokinase-2 from escherichia coli (see paper)
28% identity, 89% coverage: 2:281/315 of query aligns to 4:286/304 of 3cqdA
- active site: G253 (= G250), A254 (= A251), G255 (= G252), D256 (= D253)
- binding adenosine-5'-triphosphate: Y23 (≠ K21), G26 (≠ Q24), K27 (≠ V25), K185 (= K183), N187 (= N185), N187 (= N185), K189 (≠ H187), S224 (= S221), G226 (= G223), P227 (≠ A224), G229 (= G226), S248 (≠ V245), T251 (= T248), A254 (= A251), G255 (= G252), M258 (= M255), V280 (≠ L275), G283 (≠ A278), S284 (≠ C279)
Sites not aligning to the query:
5eynA Crystal structure of fructokinase from vibrio cholerae o395 in fructose, adp, beryllium trifluoride and calcium ion bound form
27% identity, 91% coverage: 17:302/315 of query aligns to 12:306/306 of 5eynA
- active site: G246 (= G250), A247 (= A251), G248 (= G252), D249 (= D253)
- binding adenosine-5'-diphosphate: H91 (≠ N107), T217 (≠ S221), G219 (= G223), A220 (= A224), A238 (≠ E242), V239 (≠ A243), T244 (= T248), G246 (= G250), A247 (= A251), G248 (= G252), F251 (≠ M255), N279 (≠ L277), G282 (≠ A280), A283 (= A281)
- binding beryllium trifluoride ion: G246 (= G250), G248 (= G252), D249 (= D253)
- binding beta-D-fructofuranose: D13 (= D18), G28 (= G37), A29 (≠ K38), N32 (= N41), F96 (vs. gap), F98 (= F109), R159 (≠ E172), D249 (= D253)
Sites not aligning to the query:
5yggA Crystal structure of fructokinase double-mutant (t261c-h108c) from vibrio cholerae o395 in fructose, adp and potassium ion bound form (see paper)
26% identity, 91% coverage: 17:302/315 of query aligns to 16:310/310 of 5yggA
- binding adenosine-5'-diphosphate: K188 (vs. gap), T221 (≠ S221), G223 (= G223), A242 (≠ E242), V243 (≠ A243), F255 (≠ M255), N283 (≠ L277), G286 (≠ A280), A287 (= A281)
- binding beta-D-fructofuranose: D17 (= D18), G32 (= G37), A33 (≠ K38), F100 (≠ Y101), F102 (= F109), R163 (≠ E172), D253 (= D253)
Sites not aligning to the query:
P0DX97 Deoxyribokinase; dRK; ATP:2-deoxy-D-ribose 5-phosphotransferase; EC 2.7.1.229 from Salmonella typhi (see paper)
25% identity, 94% coverage: 11:306/315 of query aligns to 11:304/306 of P0DX97
Sites not aligning to the query:
- 10 Important for substrate specificity; M→N: 2.5-fold decrease in Vmax for deoxyribokinase activity and 70-fold increase in KM for deoxyribose. 2-fold increase in KM for ribose.
Q8ZKR2 Aminoimidazole riboside kinase; AIRs kinase; EC 2.7.1.223 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
23% identity, 90% coverage: 27:308/315 of query aligns to 21:314/319 of Q8ZKR2
- G31 (= G37) binding 5-amino-1-(beta-D-ribosyl)imidazole
- Y101 (≠ D103) binding 5-amino-1-(beta-D-ribosyl)imidazole
- R162 (≠ K169) binding 5-amino-1-(beta-D-ribosyl)imidazole
- A180 (≠ I186) binding K(+)
- A181 (≠ E188) binding K(+)
- A183 (≠ K190) binding K(+)
- G213 (= G214) binding K(+)
- D246 (≠ S247) binding K(+)
- T248 (= T249) binding K(+)
- D252 (= D253) binding 5-amino-1-(beta-D-ribosyl)imidazole
- A287 (≠ K283) binding K(+)
- A290 (≠ K286) binding K(+)
- G292 (= G288) binding K(+)
Sites not aligning to the query:
- 16 binding 5-amino-1-(beta-D-ribosyl)imidazole
1tz3A Crystal structure of aminoimidazole riboside kinase complexed with aminoimidazole riboside (see paper)
24% identity, 88% coverage: 27:303/315 of query aligns to 17:298/299 of 1tz3A
- active site: C24 (≠ D34), F88 (≠ Y101), G238 (= G250), A239 (= A251), G240 (= G252), D241 (= D253)
- binding 5-aminoimidazole ribonucleoside: G27 (= G37), L83 (≠ K92), F88 (≠ Y101), Y90 (≠ D103), R151 (≠ K169), M154 (≠ L171), D241 (= D253)
Sites not aligning to the query:
1tz6A Crystal structure of aminoimidazole riboside kinase from salmonella enterica complexed with aminoimidazole riboside and atp analog (see paper)
24% identity, 88% coverage: 27:302/315 of query aligns to 17:297/297 of 1tz6A
- active site: C24 (≠ D34), F88 (≠ Y101), G238 (= G250), A239 (= A251), G240 (= G252), D241 (= D253)
- binding phosphomethylphosphonic acid adenylate ester: N149 (≠ D167), K176 (≠ D194), E181 (≠ D199), S209 (= S221), G211 (= G223), A212 (= A224), G214 (= G226), A239 (= A251), G240 (= G252), F243 (≠ M255), N270 (vs. gap), G273 (≠ A280), A274 (= A281)
- binding 5-aminoimidazole ribonucleoside: G27 (= G37), F88 (≠ Y101), Y90 (≠ D103), R151 (≠ K169), M154 (≠ L171), D241 (= D253)
Sites not aligning to the query:
Query Sequence
>WP_013431307.1 NCBI__GCF_000166775.1:WP_013431307.1
MIYTVTLNPAIDMTVYIDELKKGQVNRSNHCLIDAGGKGINVSKVIKSLGGKSIALGFLG
NDNKDWFLKYLKDLQLDFDFILVDGLTRTNIKIVETAQKVYTDLNQNGFEVKKKDINLLF
EKIDRIAKTDDIFVLSGSLPPSVDEDVYVELIRMLKRKGAKVIYDADGKALESGVLEKPD
VIKPNIHEFKCLFDVDENDLSSIVSSARRLIESGIKKVLISMGAKGAVFVTENMELFAKA
AEAKVKSTTGAGDSMVAAISYGLSQNMDDISIFKLALACAAAKVSKEGVKAPEKNEIEGF
LEKIKLERLGEFKWI
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory