SitesBLAST
Comparing WP_013444925.1 NCBI__GCF_000183135.1:WP_013444925.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
46% identity, 86% coverage: 46:330/330 of query aligns to 36:322/334 of 5aovA
- active site: L100 (≠ V111), R241 (= R249), D265 (= D273), E270 (= E278), H288 (= H296)
- binding glyoxylic acid: M52 (≠ T62), L53 (≠ F63), L53 (≠ F63), Y74 (≠ F85), A75 (≠ G86), V76 (= V87), G77 (= G88), R241 (= R249), H288 (= H296)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (= V87), T104 (= T115), F158 (≠ M165), G159 (= G166), R160 (= R167), I161 (= I168), S180 (≠ N187), R181 (= R188), A211 (≠ H219), V212 (= V220), P213 (= P221), T218 (= T226), I239 (≠ T247), A240 (= A248), R241 (= R249), H288 (= H296), G290 (= G298)
6biiA Crystal structure of pyrococcus yayanosii glyoxylate hydroxypyruvate reductase in complex with NADP and malonate (re-refinement of 5aow) (see paper)
45% identity, 96% coverage: 13:330/330 of query aligns to 1:321/332 of 6biiA
- active site: L99 (≠ V111), R240 (= R249), D264 (= D273), E269 (= E278), H287 (= H296)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V75 (= V87), T103 (= T115), G156 (= G164), F157 (≠ M165), G158 (= G166), R159 (= R167), I160 (= I168), A179 (≠ N187), R180 (= R188), S181 (≠ T189), K183 (≠ L191), V211 (= V220), P212 (= P221), E216 (= E225), T217 (= T226), V238 (≠ T247), A239 (= A248), R240 (= R249), D264 (= D273), H287 (= H296), G289 (= G298)
2dbqA Crystal structure of glyoxylate reductase (ph0597) from pyrococcus horikoshii ot3, complexed with NADP (i41) (see paper)
45% identity, 96% coverage: 13:330/330 of query aligns to 2:322/333 of 2dbqA
- active site: L100 (≠ V111), R241 (= R249), D265 (= D273), E270 (= E278), H288 (= H296)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (= V87), T104 (= T115), L158 (≠ M165), G159 (= G166), R160 (= R167), I161 (= I168), S180 (≠ N187), R181 (= R188), T182 (= T189), A211 (≠ H219), V212 (= V220), P213 (= P221), T218 (= T226), I239 (≠ T247), A240 (= A248), R241 (= R249), D265 (= D273), H288 (= H296), G290 (= G298)
O58320 Glyoxylate reductase; EC 1.1.1.26 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
45% identity, 96% coverage: 13:330/330 of query aligns to 2:322/334 of O58320
3bazA Structure of hydroxyphenylpyruvate reductase from coleus blumei in complex with NADP+ (see paper)
38% identity, 82% coverage: 55:323/330 of query aligns to 43:304/311 of 3bazA
- active site: L98 (≠ V111), R230 (= R249), A251 (= A270), D254 (= D273), E259 (= E278), H277 (= H296)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V74 (= V87), G149 (= G164), L150 (≠ M165), G151 (= G166), R152 (= R167), I153 (= I168), S172 (≠ N187), R173 (= R188), S174 (≠ T189), C201 (≠ V220), P202 (= P221), T207 (= T226), I228 (≠ T247), G229 (≠ A248), R230 (= R249), D254 (= D273), H277 (= H296), G279 (= G298)
1wwkA Crystal structure of phosphoglycerate dehydrogenase from pyrococcus horikoshii ot3
39% identity, 93% coverage: 15:322/330 of query aligns to 2:304/304 of 1wwkA
- active site: S96 (≠ V111), R230 (= R249), D254 (= D273), E259 (= E278), H278 (= H296)
- binding nicotinamide-adenine-dinucleotide: V100 (≠ T115), G146 (= G164), F147 (≠ M165), G148 (= G166), R149 (= R167), I150 (= I168), Y168 (= Y186), D169 (≠ N187), P170 (= P192), V201 (= V220), P202 (= P221), T207 (= T226), T228 (= T247), S229 (≠ A248), D254 (= D273), H278 (= H296), G280 (= G298)
Q65CJ7 Hydroxyphenylpyruvate reductase; HPPR; EC 1.1.1.237 from Plectranthus scutellarioides (Coleus) (Solenostemon scutellarioides) (see paper)
38% identity, 82% coverage: 55:323/330 of query aligns to 45:306/313 of Q65CJ7
3ddnB Crystal structure of hydroxypyruvic acid phosphate bound d-3- phosphoglycerate dehydrogenase in mycobacterium tuberculosis (see paper)
35% identity, 95% coverage: 16:330/330 of query aligns to 5:312/525 of 3ddnB
3dc2A Crystal structure of serine bound d-3-phosphoglycerate dehydrogenase from mycobacterium tuberculosis (see paper)
35% identity, 95% coverage: 16:330/330 of query aligns to 4:311/526 of 3dc2A
Sites not aligning to the query:
2gcgA Ternary crystal structure of human glyoxylate reductase/hydroxypyruvate reductase (see paper)
33% identity, 93% coverage: 15:322/330 of query aligns to 4:315/324 of 2gcgA
- active site: L103 (≠ V111), R241 (= R249), D265 (= D273), E270 (= E278), H289 (= H296)
- binding (2r)-2,3-dihydroxypropanoic acid: L55 (≠ F63), S78 (≠ G86), V79 (= V87), G80 (= G88), R241 (= R249), H289 (= H296)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: V79 (= V87), T107 (= T115), G156 (= G164), G158 (= G166), I160 (= I168), G180 (≠ N187), R181 (= R188), R184 (≠ L191), C212 (≠ V220), S213 (≠ P221), T218 (= T226), I239 (≠ T247), R241 (= R249), D265 (= D273), H289 (= H296), G291 (= G298)
Q9UBQ7 Glyoxylate reductase/hydroxypyruvate reductase; EC 1.1.1.79; EC 1.1.1.81 from Homo sapiens (Human) (see paper)
33% identity, 93% coverage: 15:322/330 of query aligns to 8:319/328 of Q9UBQ7
- VG 83:84 (= VG 87:88) binding substrate
- GRI 162:164 (= GRI 166:168) binding NADP(+)
- RQPR 185:188 (≠ RTKL 188:191) binding NADP(+)
- S217 (≠ P221) binding NADP(+)
- I243 (≠ T247) binding NADP(+)
- R245 (= R249) binding substrate
- D269 (= D273) binding substrate
- HIGS 293:296 (≠ HNGT 296:299) binding substrate
- G295 (= G298) binding NADP(+)
O43175 D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; Malate dehydrogenase; EC 1.1.1.95; EC 1.1.1.399; EC 1.1.1.37 from Homo sapiens (Human) (see 3 papers)
30% identity, 88% coverage: 40:330/330 of query aligns to 32:317/533 of O43175
- T78 (≠ V87) binding NAD(+)
- R135 (≠ V147) to W: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606949
- RI 155:156 (= RI 167:168) binding NAD(+)
- D175 (≠ N187) binding NAD(+)
- T207 (≠ V220) binding NAD(+)
- CAR 234:236 (≠ TAR 247:249) binding NAD(+)
- D260 (= D273) binding NAD(+)
- V261 (= V274) to M: in PHGDHD; results in a four-fold decrease in substrate affinity and a slight increase in maximal enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs267606947
- HLGA 283:286 (≠ HNGT 296:299) binding NAD(+)
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
- 373 A → T: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs201553627
- 377 G → S: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606948
- 425 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907988
- 490 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907987
2eklA Structure of st1218 protein from sulfolobus tokodaii
36% identity, 85% coverage: 38:317/330 of query aligns to 31:303/312 of 2eklA
- active site: S100 (≠ V111), R232 (= R249), D256 (= D273), E261 (= E278), H282 (= H296)
- binding nicotinamide-adenine-dinucleotide: I76 (≠ V87), S100 (≠ V111), G148 (= G164), G150 (= G166), R151 (= R167), I152 (= I168), Y170 (= Y186), D171 (≠ N187), I172 (≠ R188), L173 (≠ T189), H202 (= H219), V203 (= V220), T204 (≠ P221), I212 (≠ L229), T230 (= T247), S231 (≠ A248), D256 (= D273), G284 (= G298)
6plfA Crystal structure of human phgdh complexed with compound 1 (see paper)
30% identity, 86% coverage: 40:322/330 of query aligns to 28:305/305 of 6plfA
7ewhA Crystal structure of human phgdh in complex with homoharringtonine (see paper)
31% identity, 81% coverage: 40:306/330 of query aligns to 27:288/302 of 7ewhA