SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013450369.1 NCBI__GCF_000183405.1:WP_013450369.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q63XL8 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Burkholderia pseudomallei (strain K96243) (see paper)
57% identity, 98% coverage: 3:312/315 of query aligns to 7:315/318 of Q63XL8

query
sites
Q63XL8

F

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V

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G

N

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RQ 99:100 (= RQ 95:96) binding ATP

P14193 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PPRibP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Bacillus subtilis (strain 168) (see 4 papers)
53% identity, 99% coverage: 2:312/315 of query aligns to 9:316/317 of P14193

query
sites
P14193

D

N

F

L

L

K

V

I

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RQ 102:103 (= RQ 95:96) binding ATP
K198 (= K193) mutation to A: Strong decrease of the Vmax value compared to that of the wild-type. The affinity binding for ATP and Rib-5-P are slightly altered compared to the wild-type. The cooperativity of ADP binding is reduced.
R200 (= R195) mutation to A: Strong decrease of the Vmax value compared to that of the wild-type enzyme. The affinity binding for ATP and Rib-5-P are slightly altered compared to the wild-type.
R202 (≠ G197) mutation to A: 3-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
N204 (= N199) mutation to A: 4.5-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
E207 (≠ K202) mutation to A: 2.5-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
DTAGT 228:232 (= DTAGT 223:227) binding D-ribose 5-phosphate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

3dahC 2.3 a crystal structure of ribose-phosphate pyrophosphokinase from burkholderia pseudomallei (see paper)
56% identity, 97% coverage: 3:308/315 of query aligns to 2:299/300 of 3dahC

query
sites
3dahC

F

L

L

M

V

V

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F

S

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G

G

N

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binding adenosine monophosphate: F33 (= F34), D35 (= D36), E37 (= E38), R94 (= R95), Q95 (= Q96)

P0A717 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Escherichia coli (strain K12) (see 4 papers)
53% identity, 99% coverage: 2:312/315 of query aligns to 3:313/315 of P0A717

query
sites
P0A717

D

D

F

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E

A

A

I

I

N

R

R

R

I

I

Y

S

K

N

K

E

E

E

S

S

I

I

S

S

S

A

L

M

F

F

D129 (= D127) to A: in mutant PRSA1; alters the binding of divalent cations, especially magnesium. Little alteration in the affinity for ribose 5-phosphate and 27-fold decrease of the affinity for ATP. Absence of inhibition by AMP
D220 (= D219) mutation to E: 4-fold decrease in the affinity binding for Rib-5-P in the presence of magnesium ions. In the presence of cobalt ions, it shows a 15-fold decrease in the affinity binding for Rib-5-P.; mutation to F: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.
D221 (= D220) mutation to A: The affinity binding for ATP is comparable to those of the wild-type, apart from a slight decrease in the presence of manganese ions. The affinity binding for Rib-5-P is greatly decreased in the presence of both manganese and cobalt ions but only about 2-fold in the presence of magnesium ions.
D224 (= D223) mutation to A: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.; mutation to S: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

6asvC E. Coli prpp synthetase (see paper)
53% identity, 99% coverage: 2:312/315 of query aligns to 1:311/311 of 6asvC

query
sites
6asvC

D

D

F

M

L

K

V

L

F

F

S

A

G

G

N

N

S

A

N

T

K

P

T

E

L

L

A

A

Q

Q

G

R

I

I

V

A

A

N

K

R

L

L

G

Y

L

T

R

S

L

L

G

G

D

D

A

A

T

A

V

V

S

G

K

R

F

F

S

S

D

D

G

G

E

E

I

V

F

S

V

V

R

Q

I

I

N

N

E

E

S

N

V

V

R

R

G

G

R

G

D

D

V

I

F

F

V

I

Q

Q

S

S

T

T

N

C

Y

A

P

P

A

T

E

N

V

D

H

N

L

L

M

M

E

E

L

L

M

V

I

V

M

M

V

V

D

D

A

A

L

L

K

R

R

R

A

A

S

S

A

A

K

G

S

R

V

I

T

T

A

A

V

V

M

I

P

P

Y

Y

F

F

G

G

Y

Y

A

A

R

R

Q

Q

D

D

R

R

T

R

V

V

E

R

P

S

-

A

R

R

V

V

P

P

I

I

T

T

A

A

K

K

L

V

V

V

A

A

N

D

L

F

L

L

T

S

K

S

S

V

G

G

I

V

D

D

R

R

I

V

V

L

T

T

M

V

D

D

L

L

H

H

A

A

G

E

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

V

P

P

V

V

D

D

N

N

L

V

Y

F

S

G

V

S

P

P

I

I

I

L

A

L

K

E

Y

D

F

M

R

L

D

Q

K

L

G

N

M

L

C

-

G

-

E

D

D

N

Y

P

V

I

V

V

S

S

P

P

D
|
D

A

I

G

G

V

V

T

V

R

R

A

A

R

R

G

A

F

I

A

A

K

K

A

L

L

L

-

N

D

D

T

T

S

D

L

M

A

A

I

I

D

D

K

K

R

R

S

P

G

R

P

A

N

N

V

V

A

S

K

Q

A

V

M

M

N

H

V

I

I

I

G

G

D

D

V

V

K

A

G

G

K

R

G

D

V

C

I

V

I

L

I

V

D
|
D

M

M

I

I

D
|
D

T
|
T

A
x
G

G

G

T
|
T

L
|
L

V

C

E

K

A

A

H

E

A

A

V

L

L

K

E

E

H

R

G

G

A

A

T

K

K

R

V

V

V

F

A

A

G

Y

A

A

S

T

H

H

G

P

I

I

L

F

S

S

G

G

P

N

A

A

I

A

E

N

R

N

I

L

L

R

K

N

S

S

E

V

L

I

E

D

E

E

V

V

I

V

T

C

D

D

T

T

I

I

E

P

A

L

S

S

K

D

E

E

K

I

L

K

S

S

F

L

S

P

K

N

L

V

K

R

I

T

L

L

S

T

T

L

S

S

D

G

L

M

F

L

A

A

E

E

A

A

I

I

N

R

R

R

I

I

Y

S

K

N

K

E

E

E

S

S

I

I

S

S

S

A

L

M

F

F

binding phosphate ion: D168 (= D170), D218 (= D219), D219 (= D220), D222 (= D223), T223 (= T224), G224 (≠ A225), T226 (= T227), L227 (= L228)

1dkuA Crystal structures of bacillus subtilis phosphoribosylpyrophosphate synthetase: molecular basis of allosteric inhibition and activation. (see paper)
52% identity, 98% coverage: 5:312/315 of query aligns to 4:295/295 of 1dkuA

query
sites
1dkuA

V

I

F

F

S

S

G

L

N

N

S

S

N

N

K

P

T

E

L

L

A

A

Q

K

G

E

I

I

V

A

A

D

K

I

L

V

G

G

L

V

R

Q

L

L

G

G

D

K

A

C

T

S

V

V

S

T

K

R

F

F

S

S

D

D

G

G

E

E

I

V

F

Q

V

I

R

N

I

I

N

E

E

E

S

S

V

I

R

R

G

G

R

C

D

D

V

C

F

Y

V

I

Q

Q

S

S

T

T

N

S

Y

D

P

P

A

V

E

N

V

E

H

H

L

I

M

M

E

E

L

L

M

L

I

I

M

M

V

V

D

D

A

A

L

L

K

K

R

R

A

A

S

S

A

A

K

K

S

T

V

I

T

N

A

I

V

V

M

I

P

P

Y

Y

F

Y

G

G

Y

Y

A

A

R
|
R

Q

Q

D

D

R

R

T
x
K

V

A

E

R

P
x
S

R
|
R

V

E

P

P

I

I

T

T

A

A

K

K

L

L

V

F

A

A

N

N

L

L

T

E

K

T

S

A

G

G

I

A

D

T

R

R

I

V

V

I

T

A

M

L

D

D

L

L

H
|
H

A

A

G

P

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

I

D
|
D

N

H

L

L

Y

M

S

G

V

V

P

P

I

I

I

L

A

G

K

E

Y

Y

F

F

R

E

D

G

K

K

G

N

M

L

C

-

G

-

E

E

D

D

Y

I

V

V

V

I

V

V

S

S

P

P

D

D

A

H

G

G

V

V

T

T

R

R

A

A

R

R

G

K

F

L

A

A

K

D

A

R

L

L

D

K

T

A

S

P

L

I

A

A

I

I

D

D

K

K

R

R

R

-

S

-

G

-

P

-

N

-

V

-

A

-

K

-

A

-

M

M

N

N

V

I

I

V

G

G

D

N

V

I

K

E

G

G

K

K

G

T

V

A

I

I

I

L

I

I

D

D

M

I

I

I

D

D

T

T

A

A

G

G

T

T

L

I

V

T

E

L

A

A

H

N

A

A

V

L

L

V

E

E

H

N

G

G

A

A

T

K

K

E

V

V

V

Y

A

A

G

C

A

C

S

T

H

H

G

P

I

V

L

L

S

S

G

G

P

P

A

A

I

V

E

E

R

R

I

I

L

N

K

N

S

S

E

T

L

I

E

K

E

E

V

L

V

V

I

V

T

T

D

N

T

S

I

I

E

-

A

-

S

-

K

-

E

-

K

K

L

L

S

K

F

I

S

E

K

R

L

F

K

K

I

Q

L

L

S

S

T

V

S

G

D

P

L

L

F

L

A

A

E

E

A

A

I

I

N

I

R

R

I

V

Y

H

K

E

K

Q

E

Q

S
|
S

I
x
V

S
|
S

S

Y

L

L

F
|
F

binding methyl phosphonic acid adenosine ester: R94 (= R95), H128 (= H129)
binding phosphomethylphosphonic acid adenosyl ester: K98 (≠ T99), S101 (≠ P102), R102 (= R103), D141 (= D142), S290 (= S307), V291 (≠ I308), S292 (= S309), F295 (= F312)

1ibsA Phosphoribosyldiphosphate synthetase in complex with cadmium ions (see paper)
52% identity, 98% coverage: 5:312/315 of query aligns to 4:297/297 of 1ibsA

query
sites
1ibsA

V

I

F

F

S

S

G

L

N

N

S

S

N

N

K

P

T

E

L

L

A

A

Q

K

G

E

I

I

V

A

A

D

K

I

L

V

G

G

L

V

R

Q

L

L

G

G

D

K

A

C

T

S

V

V

S

T

K

R

F

F

S

S

D

D

G

G

E

E

I

V

F

Q

V

I

R

N

I

I

N

E

E

E

S

S

V

I

R

R

G

G

R

C

D

D

V

C

F

Y

V

I

Q

Q

S

S

T

T

N

S

Y

D

P

P

A

V

E

N

V

E

H

H

L

I

M

M

E

E

L

L

M

L

I

I

M

M

V

V

D

D

A

A

L

L

K

K

R

R

A

A

S

S

A

A

K

K

S

T

V

I

T

N

A

I

V

V

M

I

P

P

Y

Y

F

Y

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
|
R

T

-

V

-

E

K

P

S

R

R

V

E

P

P

I

I

T

T

A

A

K

K

L

L

V

F

A

A

N

N

L

L

T

E

K

T

S

A

G

G

I

A

D

T

R

R

I

V

V

I

T

A

M

L

D

D

L

L

H
|
H

A

A

G

P

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

I

D

D

N

H

L

L

Y

M

S

G

V

V

P

P

I

I

I

L

A

G

K

E

Y

Y

F

F

R

E

D

G

K

K

G

N

M

L

C

-

G

-

E

E

D

D

Y

I

V

V

V

I

V

V

S

S

P

P

D

D

A

H

G

G

V

V

T

T

R

R

A

A

R

R

G

K

F

L

A

A

K

D

A

R

L

L

D

K

T

A

S

P

L

I

A

A

I

I

D

D

K

K

R

R

R

-

S

-

G

-

P

-

N

-

V

-

A

-

K

-

A

-

M

M

N

N

V

I

I

V

G

G

D

N

V

I

K

E

G

G

K

K

G

T

V

A

I

I

I

L

I

I

D

D

M

I

I

I

D
|
D

T
|
T

A
|
A

G

G

T
|
T

L

I

V

T

E

L

A

A

H

N

A

A

V

L

L

V

E

E

H

N

G

G

A

A

T

K

K

E

V

V

V

Y

A

A

G

C

A

C

S

T

H

H

G

P

I

V

L

L

S

S

G

G

P

P

A

A

I

V

E

E

R

R

I

I

L

N

K

N

S

S

E

T

L

I

E

K

E

E

V

L

V

V

I

V

T

T

D

N

T

S

I

I

E

K

A

L

S

P

K

E

E

E

K

K

L

-

S

K

F

I

S

E

K

R

L

F

K

K

I

Q

L

L

S

S

T

V

S

G

D

P

L

L

F

L

A

A

E

E

A

A

I

I

N

I

R

R

I

V

Y

H

K

E

K

Q

E

Q

S

S

I

V

S

S

S

Y

L

L

F

F

binding methyl phosphonic acid adenosine ester: R94 (= R95), Q95 (= Q96), R97 (= R98), H126 (= H129)
binding sulfate ion: D209 (= D223), T210 (= T224), A211 (= A225), T213 (= T227)

1ibsB Phosphoribosyldiphosphate synthetase in complex with cadmium ions (see paper)
52% identity, 98% coverage: 5:312/315 of query aligns to 6:299/299 of 1ibsB

query
sites
1ibsB

V

I

F

F

S

S

G

L

N

N

S

S

N

N

K

P

T

E

L

L

A

A

Q

K

G

E

I

I

V

A

A

D

K

I

L

V

G

G

L

V

R

Q

L

L

G

G

D

K

A

C

T

S

V

V

S

T

K

R

F

F

S

S

D

D

G

G

E

E

I

V

F

Q

V

I

R

N

I

I

N

E

E

E

S

S

V

I

R

R

G

G

R

C

D

D

V

C

F

Y

V

I

Q

Q

S

S

T

T

N

S

Y

D

P

P

A

V

E

N

V

E

H

H

L

I

M

M

E

E

L

L

M

L

I

I

M

M

V

V

D

D

A

A

L

L

K

K

R

R

A

A

S

S

A

A

K

K

S

T

V

I

T

N

A

I

V

V

M

I

P

P

Y

Y

F

Y

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
|
R

T

-

V

-

E

K

P

S

R

R

V

E

P

P

I

I

T

T

A

A

K

K

L

L

V

F

A

A

N

N

L

L

T

E

K

T

S

A

G

G

I

A

D

T

R

R

I

V

V

I

T

A

M

L

D

D

L

L

H

H

A

A

G

P

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

I

D

D

N

H

L

L

Y

M

S

G

V

V

P

P

I

I

I

L

A

G

K

E

Y

Y

F

F

R

E

D

G

K

K

G

N

M

L

C

-

G

-

E

E

D

D

Y

I

V

V

V

I

V

V

S

S

P

P

D

D

A

H

G

G

V

V

T

T

R

R

A

A

R

R

G

K

F

L

A

A

K

D

A

R

L

L

D

K

T

A

S

P

L

I

A

A

I

I

D

D

K

K

R

R

R

-

S

-

G

-

P

-

N

-

V

-

A

-

K

-

A

-

M

M

N

N

V

I

I

V

G

G

D

N

V

I

K

E

G

G

K

K

G

T

V

A

I

I

I

L

I

I

D

D

M

I

I

I

D

D

T

T

A

A

G

G

T

T

L

I

V

T

E

L

A

A

H

N

A

A

V

L

L

V

E

E

H

N

G

G

A

A

T

K

K

E

V

V

V

Y

A

A

G

C

A

C

S

T

H

H

G

P

I

V

L

L

S

S

G

G

P

P

A

A

I

V

E

E

R

R

I

I

L

N

K

N

S

S

E

T

L

I

E

K

E

E

V

L

V

V

I

V

T

T

D

N

T

S

I

I

E

K

A

L

S

P

K

E

E

E

K

K

L

-

S

K

F

I

S

E

K

R

L

F

K

K

I

Q

L

L

S

S

T

V

S

G

D

P

L

L

F

L

A

A

E

E

A

A

I

I

N

I

R

R

I

V

Y

H

K

E

K

Q

E

Q

S

S

I

V

S

S

S

Y

L

L

F

F

binding methyl phosphonic acid adenosine ester: R96 (= R95), Q97 (= Q96), R99 (= R98)

4s2uA Crystal structure of the phosphorybosylpyrophosphate synthetase from e. Coli
53% identity, 97% coverage: 2:308/315 of query aligns to 2:308/308 of 4s2uA

query
sites
4s2uA

D

D

F

M

L

K

V

L

F

F

S

A

G

G

N

N

S

A

N

T

K

P

T

E

L

L

A

A

Q

Q

G

R

I

I

V

A

A

N

K

R

L

L

G

Y

L

T

R

S

L

L

G

G

D

D

A

A

T

A

V

V

S

G

K

R

F

F

S

S

D

D

G

G

E

E

I

V

F

S

V

V

R

Q

I

I

N

N

E

E

S

N

V

V

R

R

G

G

R

G

D

D

V

I

F

F

V

I

Q

Q

S

S

T

T

N

C

Y

A

P

P

A

T

E

N

V

D

H

N

L

L

M

M

E

E

L

L

M

V

I

V

M

M

V

V

D

D

A

A

L

L

K

R

R

R

A

A

S

S

A

A

K

G

S

R

V

I

T

T

A

A

V

V

M

I

P

P

Y

Y

F

F

G

G

Y

Y

A

A

R

R

Q

Q

D

D

R

R

T

R

V

V

E

R

P

S

-

A

R

R

V

V

P

P

I

I

T

T

A

A

K

K

L

V

V

V

A

A

N

D

L

F

L

L

T

S

K

S

S

V

G

G

I

V

D

D

R

R

I

V

V

L

T

T

M

V

D

D

L

L

H

H

A

A

G

E

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

V

P

P

V

V

D

D

N

N

L

V

Y

F

S

G

V

S

P

P

I

I

I

L

A

L

K

E

Y

D

F

M

R

L

D

Q

K

L

G

N

M

L

C

-

G

-

E

D

D

N

Y

P

V

I

V

V

S

S

P

P

D

D

A

I

G

G

V

V

T

V

R

R

A

A

R

R

G

A

F

I

A

A

K

K

A

L

L

L

-

N

D

D

T

T

S

D

L

M

A

A

I

I

D

D

K

K

R

R

S

P

G

R

P

A

N

N

V

V

A

S

K

Q

A

V

M

M

N

H

V

I

I

I

G

G

D

D

V

V

K

A

G

G

K

R

G

D

V

C

I

V

I

L

I

V

D

D

M

M

I

I

D
|
D

T

T

A

G

G

G

T
|
T

L

L

V

C

E

K

A

A

H

E

A

A

V

L

L

K

E

E

H

R

G

G

A

A

T

K

K

R

V

V

V

F

A

A

G

Y

A

A

S

T

H

H

G

P

I

I

L

F

S

S

G

G

P

N

A

A

I

A

E

N

R

N

I

L

L

R

K

N

S

S

E

V

L

I

E

D

E

E

V

V

I

V

T

C

D

D

T

T

I

I

E

P

A

L

S

S

K

D

E

E

K

I

L

K

S

S

F

L

S

P

K

N

L

V

K

R

I

T

L

L

S

T

T

L

S

S

D

G

L

M

F

L

A

A

E

E

A

A

I

I

N

R

R

R

I

I

Y

S

K

N

K

E

E

E

S

S

I

I

binding magnesium ion: D223 (= D223), T227 (= T227)

7xmvA E.Coli phosphoribosylpyrophosphate (prpp) synthetase type a(amp/adp) filament bound with adp, amp and r5p (see paper)
53% identity, 99% coverage: 2:312/315 of query aligns to 1:305/307 of 7xmvA

query
sites
7xmvA

D

D

F

M

L

K

V

L

F

F

S

A

G

G

N

N

S

A

N

T

K

P

T

E

L

L

A

A

Q

Q

G

R

I

I

V

A

A

N

K

R

L

L

G

Y

L

T

R

S

L

L

G

G

D

D

A

A

T

A

V

V

S

G

K

R

F
|
F

S

S

D
|
D

G

G

E
|
E

I

V

F

S

V

V

R

Q

I

I

N

N

E

E

S

N

V

V

R

R

G

G

R

G

D

D

V

I

F

F

V

I

Q

Q

S

S

T

T

N

C

Y

A

P

P

A

T

E

N

V

D

H

N

L

L

M

M

E

E

L

L

M

V

I

V

M

M

V

V

D

D

A

A

L

L

K

R

R

R

A

A

S

S

A

A

K

G

S

R

V

I

T

T

A

A

V

V

M

I

P

P

Y

Y

F

F

G

G

Y

Y

A

A

R
|
R

Q

Q

D

D

R
|
R

T

R

V
|
V

E
x
R

P

S

-

A

R

R

V

V

P

P

I

I

T

T

A

A

K

K

L

V

V

V

A

A

N

D

L

F

L

L

T

S

K

S

S

V

G

G

I

V

D

D

R

R

I

V

V

L

T

T

M

V

D

D

L

L

H
|
H

A

A

G
x
E

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

V

P

P

V

V

D

D

N

N

L

V

Y
x
F

S

G

V
x
S

P

P

I

I

I

L

A

L

K

E

Y

D

F

M

R

L

D

Q

K

L

G

N

M

L

C

-

G

-

E

D

D

N

Y

P

V

I

V

V

S

S

P

P

D

D

A

I

G

G

V

V

T
x
V

R

R

A

A

R

R

G
x
A

F

I

A

A

K

K

A

L

L

L

-

N

D

D

T

T

S

D

L

M

A

A

I

I

D

D

K

K

R

R

S

-

G

-

P

-

N

-

V

-

A

-

K

Q

A

V

M

M

N

H

V

I

I

I

G

G

D

D

V

V

K

A

G

G

K

R

G

D

V

C

I

V

I

L

I

V

D
|
D

M
|
M

I

I

D
|
D

T
|
T

A

G

G
|
G

T
|
T

L

L

V

C

E

K

A

A

H

E

A

A

V

L

L

K

E

E

H

R

G

G

A

A

T

K

K

R

V

V

V

F

A

A

G

Y

A

A

S

T

H

H

G

P

I

I

L

F

S

S

G

G

P

N

A

A

I

A

E

N

R

N

I

L

L

R

K

N

S

S

E

V

L

I

E

D

E

E

V

V

I

V

T

C

D

D

T

T

I

I

E

P

A

L

S

S

K

D

E

E

K

I

L

K

S

S

F

L

S

P

K

N

L

V

K

R

I

T

L

L

S

T

T

L

S

S

D

G

L

M

F

L

A

A

E

E

A

A

I

I

N

R

R

R

I

I

Y

S

K

N

K

E

E

E

S

S

I

I

S

S

S

A

L

M

F

F

binding adenosine-5'-diphosphate: F33 (= F34), D35 (= D36), E37 (= E38), R94 (= R95), R97 (= R98), H129 (= H129)
binding adenosine monophosphate: R97 (= R98), V99 (= V100), R100 (≠ E101), E131 (≠ G131), F145 (≠ Y145), S147 (≠ V147), V173 (≠ T175), A177 (≠ G179)
binding 5-O-phosphono-alpha-D-ribofuranose: D212 (= D219), D213 (= D220), M214 (= M221), D216 (= D223), T217 (= T224), G219 (= G226), T220 (= T227)

7xmuA E.Coli phosphoribosylpyrophosphate (prpp) synthetase type a filament bound with adp, pi and r5p (see paper)
53% identity, 99% coverage: 2:312/315 of query aligns to 1:305/307 of 7xmuA

query
sites
7xmuA

D

D

F

M

L

K

V

L

F

F

S

A

G

G

N

N

S

A

N

T

K

P

T

E

L

L

A

A

Q

Q

G

R

I

I

V

A

A

N

K

R

L

L

G

Y

L

T

R

S

L

L

G

G

D

D

A

A

T

A

V

V

S

G

K

R

F
|
F

S

S

D
|
D

G

G

E
|
E

I

V

F

S

V

V

R

Q

I

I

N

N

E

E

S

N

V

V

R

R

G

G

R

G

D

D

V

I

F

F

V

I

Q

Q

S

S

T

T

N

C

Y

A

P

P

A

T

E

N

V

D

H

N

L

L

M

M

E

E

L

L

M

V

I

V

M

M

V

V

D

D

A

A

L

L

K

R

R

R

A

A

S

S

A

A

K

G

S

R

V

I

T

T

A

A

V

V

M

I

P

P

Y

Y

F

F

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
|
R

T

R

V

V

E
x
R

P

S

-

A

R

R

V

V

P

P

I

I

T

T

A

A

K

K

L

V

V

V

A

A

N

D

L

F

L

L

T

S

K

S

S

V

G

G

I

V

D

D

R

R

I

V

V

L

T

T

M

V

D

D

L

L

H
|
H

A

A

G
x
E

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

V

P

P

V

V

D

D

N

N

L

V

Y
x
F

S

G

V
x
S

P

P

I

I

I

L

A

L

K

E

Y

D

F

M

R

L

D

Q

K

L

G

N

M

L

C

-

G

-

E

D

D

N

Y

P

V

I

V

V

S

S

P

P

D
|
D

A

I

G

G

V

V

T
x
V

R

R

A

A

R

R

G

A

F

I

A

A

K

K

A

L

L

L

-

N

D

D

T

T

S

D

L

M

A

A

I

I

D

D

K

K

R

R

S

-

G

-

P

-

N

-

V

-

A

-

K

Q

A

V

M

M

N

H

V

I

I

I

G

G

D

D

V

V

K

A

G

G

K

R

G

D

V

C

I

V

I

L

I

V

D
|
D

D

D

M
|
M

I

I

D
|
D

T
|
T

A

G

G

G

T

T

L

L

V

C

E

K

A

A

H

E

A

A

V

L

L

K

E

E

H

R

G

G

A

A

T

K

K

R

V

V

V

F

A

A

G

Y

A

A

S

T

H

H

G

P

I

I

L

F

S

S

G

G

P

N

A

A

I

A

E

N

R

N

I

L

L

R

K

N

S

S

E

V

L

I

E

D

E

E

V

V

I

V

T

C

D

D

T

T

I

I

E

P

A

L

S

S

K

D

E

E

K

I

L

K

S

S

F

L

S

P

K

N

L

V

K

R

I

T

L

L

S

T

T

L

S

S

D

G

L

M

F

L

A

A

E

E

A

A

I

I

N

R

R

R

I

I

Y

S

K

N

K

E

E

E

S

S

I

I

S

S

S

A

L

M

F

F

binding adenosine-5'-diphosphate: F33 (= F34), D35 (= D36), E37 (= E38), R94 (= R95), Q95 (= Q96), R97 (= R98), R97 (= R98), R100 (≠ E101), H129 (= H129), E131 (≠ G131), F145 (≠ Y145), S147 (≠ V147), V173 (≠ T175)
binding 5-O-phosphono-alpha-D-ribofuranose: D168 (= D170), D212 (= D219), M214 (= M221), D216 (= D223), T217 (= T224)

6nfeB Crystal structure of ribose-phosphate pyrophosphokinase from legionella pneumophila with bound amp, adp, and ribose-5-phosphate
52% identity, 95% coverage: 3:302/315 of query aligns to 3:297/299 of 6nfeB

query
sites
6nfeB

F

M

L

M

V

I

F

F

S

T

G

G

N

N

S

A

N

N

K

P

T

E

L

L

A

A

Q

L

G

K

I

I

V

S

A

S

K

H

L

L

G

Q

L

I

R

P

L

I

G

G

D

K

A

A

T

T

V

V

S

G

K

T

F
|
F

S

S

D
|
D

G

G

E
|
E

I

T

F

M

V

V

R

E

I

I

N

L

E

E

S

N

V

V

R

R

G

G

R

K

D

D

V

V

F

F

V

V

V

L

Q

Q

S

S

T

T

N

C

Y

A

P

P

A

A

E

N

V

N

H

N

L

L

M

M

E

E

L

L

M

L

I

I

M

M

V

A

D

D

A

A

L

L

K

R

R

R

A

S

S

S

A

A

K

G

S

R

V

I

T

T

A

A

V

V

M

V

P

P

Y

Y

F

F

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R

R

T

R

V

V

E

R

P

S

-

A

R

R

V

V

P

P

I

I

T

T

A

A

K

K

L

V

V

V

A

A

N

D

L

M

T

A

K

S

S

V

G

G

I

I

D

C

R

R

I

V

V

L

T

T

M

V

D

D

L

L

H
|
H

A

A

G

D

Q

Q

I

I

Q

Q

G

G

F

F

D

Y

I

M

P

P

V

V

D

D

N

N

L

V

Y

Y

S

S

V

T

P

P

I

V

I

L

A

L

K

E

Y

D

F

I

R

T

D

K

K

Q

G

K

M

L

C

-

G

-

E

N

D

N

Y

I

V

M

V

I

V

V

S

S

P

P

D

D

A

V

G

G

V

V

T

V

R

R

A

A

R

R

G

A

F

V

A

A

K

K

A

R

L

L

-

N

D

D

T

A

S

E

L

L

A

S

I

I

D

D

K

K

R

R

S

S

G

-

P

-

N

-

V

-

A

-

K

E

A

V

M

M

N

H

V

I

I

I

G

G

D

E

V

P

K

A

G

N

K

K

G

N

V

C

I

I

I

V

D
|
D

M
x
I

I

V

D
|
D

T
|
T

A
|
A

G

G

T
|
T

L

L

V

C

E

T

A

A

H

H

A

E

V

L

L

K

E

K

H

N

G

G

A

A

T

K

K

S

V

V

V

R

A

A

G

Y

A

I

S

T

H

H

G

P

I

V

L

L

S

S

G

G

P

P

A

A

I

V

E

N

R

N

I

I

L

K

K

H

S

S

E

G

L

L

E

D

E

E

V

V

I

V

T

T

D

D

T

T

I

I

E

P

A

L

S

S

K

A

E

E

K

A

L

Q

S

N

F

C

S

E

K

K

L

I

K

R

I

V

L

V

S

S

T

L

S

A

D

D

L

M

F

L

A

A

E

Q

A

A

I

I

N

K

R

R

I

V

binding adenosine-5'-diphosphate: F34 (= F34), D36 (= D36), E38 (= E38), R95 (= R95), Q96 (= Q96), H130 (= H129)
binding 5-O-phosphono-alpha-D-ribofuranose: H130 (= H129), D214 (= D219), D215 (= D220), I216 (≠ M221), D218 (= D223), T219 (= T224), A220 (= A225), T222 (= T227)

6nfeA Crystal structure of ribose-phosphate pyrophosphokinase from legionella pneumophila with bound amp, adp, and ribose-5-phosphate
51% identity, 95% coverage: 3:302/315 of query aligns to 3:296/298 of 6nfeA

query
sites
6nfeA

F

M

L

M

V

I

F

F

S

T

G

G

N

N

S

A

N

N

K

P

T

E

L

L

A

A

Q

L

G

K

I

I

V

S

A

S

K

H

L

L

G

Q

L

I

R

P

L

I

G

G

D

K

A

A

T

T

V

V

S

G

K

T

F
|
F

S

S

D
|
D

G

G

E
|
E

I

T

F

M

V

V

R

E

I

I

N

L

E

E

S

N

V

V

R

R

G

G

R

K

D

D

V

V

F

F

V

V

V

L

Q

Q

S

S

T

T

N

C

Y

A

P

P

A

A

E

N

V

N

H

N

L

L

M

M

E

E

L

L

M

L

I

I

M

M

V

A

D

D

A

A

L

L

K

R

R

R

A

S

S

S

A

A

K

G

S

R

V

I

T

T

A

A

V

V

M

V

P

P

Y

Y

F

F

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
|
R

T

R

V
|
V

E

R

P

S

-

A

R

R

V

V

P

P

I

I

T

T

A

A

K

K

L

V

V

V

A

A

N

D

L

M

T

A

K

S

S

V

G

G

I

I

D

C

R

R

I

V

V

L

T

T

M

V

D

D

L

L

H
|
H

A

A

G

D

Q

Q

I

I

Q

Q

G

G

F

F

D

Y

I

M

P

P

V

V

D

D

N

N

L

V

Y
|
Y

S

S

V

T

P

P

I

V

I

L

A

L

K

E

Y

D

F

I

R

T

D

K

K

Q

G

K

M

L

C

-

G

-

E

N

D

N

Y

I

V

M

V

I

V

V

S

S

P

P

D

D

A

V

G

G

V

V

T

V

R
|
R

A

A

R

R

G
x
A

F

V

A

A

K
|
K

A

R

L

L

-

N

D

D

T

A

S

E

L

L

A

S

I

I

D

D

K

K

R

R

S

-

G

-

P

-

N

-

V

-

A

-

K

E

A

V

M

M

N

H

V

I

I

I

G

G

D

E

V

P

K

A

G

N

K

K

G

N

V

C

I

I

I

V

D
|
D

M
x
I

I

V

D
|
D

T
|
T

A
|
A

G

G

T
|
T

L

L

V

C

E

T

A

A

H

H

A

E

V

L

L

K

E

K

H

N

G

G

A

A

T

K

K

S

V

V

V

R

A

A

G

Y

A

I

S

T

H

H

G

P

I

V

L

L

S

S

G

G

P

P

A

A

I

V

E

N

R

N

I

I

L

K

K

H

S

S

E

G

L

L

E

D

E

E

V

V

I

V

T

T

D

D

T

T

I

I

E

P

A

L

S

S

K

A

E

E

K

A

L

Q

S

N

F

C

S

E

K

K

L

I

K

R

I

V

L

V

S

S

T

L

S

A

D

D

L

M

F

L

A

A

E

Q

A

A

I

I

N

K

R

R

I

V

binding adenosine-5'-diphosphate: F34 (= F34), D36 (= D36), E38 (= E38), R95 (= R95), Q96 (= Q96), H130 (= H129)
binding adenosine monophosphate: R98 (= R98), V100 (= V100), Y146 (= Y145), R175 (= R176), A178 (≠ G179), K181 (= K182)
binding 5-O-phosphono-alpha-D-ribofuranose: H130 (= H129), D213 (= D219), D214 (= D220), I215 (≠ M221), D217 (= D223), T218 (= T224), A219 (= A225), T221 (= T227)

5t3oA Crystal structure of the phosphorybosylpyrophosphate synthetase ii from thermus thermophilus (see paper)
50% identity, 98% coverage: 3:312/315 of query aligns to 2:306/307 of 5t3oA

query
sites
5t3oA

F

L

L

L

V

I

F

F

S

S

G

G

N

Q

S

S

N

N

K

R

T

P

L

L

A

A

Q

Q

G

A

I

I

V

A

A

E

K

A

L

L

G

G

L

L

R

P

L

L

G

G

D

K

A

S

T

T

V

T

S

L

K

R

F

F

S

A

D

N

G

D

E

N

I

L

F

F

V

V

R

R

I

Y

N

E

E

E

S
|
S

V

L

R
|
R

G

E

R

G

D

D

V

V

F

F

V

I

V

V

Q

Q

S

S

T

F

N

V

Y

P

P

P

A

V

E

Q

V

D

H

H

L

L

M

M

E

E

L

L

M

L

I

M

M

M

V

V

D

D

A

A

L

A

K

K

R

G

A

A

S

S

A

A

K

A

S

R

V

V

T

T

A

A

V

V

M

I

P

P

Y

Y

F

F

G

S

Y

Y

A

A

R
|
R

Q
x
S

D

D

R

K

T

K

V
x
D

E

A

P

P

R

R

V

I

P

S

I

I

T

T

A

A

K

R

L

L

V

I

A

A

N

D

L

L

T

Q

K

T

S

A

G

G

I

A

D

D

R

R

I

V

V

L

T

T

M

M

D

T

L

L

H
|
H

A

S

G

P

Q

Q

I

V

Q

H

G

G

F

F

D

K

I

I

P

P

V

V

D

D

N

H

L

L

Y

S

S

A

V

E

P

P

I

V

I

I

A

A

K

N

Y

Y

F

F

R

A

D

T

K

R

G

-

M

V

C

D

G

L

E

E

D

N

Y

A

V

V

S

A

P

P

D

D

A

A

G

G

G

D

V

L

T

K

R

R

A

A

R

S

G

A

F

L

A

A

K

R

A

R

L

L

D

G

T

L

S

P

L

L

A

A

I

F

I

I

D

D

K

K

R

E

R
|
R

S

V

G

S

P

D

N

T

V

E

A

V

K

R

A

V

M

R

N

M

V

L

I

V

G

G

D

E

V

V

K

E

G

G

K

K

G

T

V

A

I

L

I

I

I

V

D

D

M

E

I

I

D

S

T

T

A

A

G

G

T

S

L

L

V

V

E

E

A

A

A

V

H

E

A

A

V

L

L

M

E

Q

H

A

G

G

A

A

T

K

K

E

V

V

V

Y

A

A

G

A

A

A

S

T

H

H

G

G

I

V

L

Y

S

V

G

G

P

P

A

A

I

L

E

D

R

R

I

I

L

A

K

K

S

S

E

P

L

V

E

K

E

E

V

V

V

A

I

A

T

T

D

D

T

T

I

C

E

P

A

-

S

P

K

K

E

E

K

G

L

-

S

-

F

-

S

P

K

K

L

L

K

R

I

T

L

L

S

T

T

V

S

A

D

P

L

L

F

F

A

A

E

E

A

A

I

I

N

W

R

R

I

I

Y

H

K

R

K

G

E

E

S

S

I

V

S

S

L

L

F

F

binding adenosine-5'-diphosphate: R94 (= R95), S95 (≠ Q96), D99 (≠ V100), H128 (= H129), R193 (= R195)
binding sulfate ion: S45 (= S46), R47 (= R48)

7pn0A Crystal structure of the phosphorybosylpyrophosphate synthetase ii from thermus thermophilus at r32 space group
50% identity, 98% coverage: 3:312/315 of query aligns to 3:307/312 of 7pn0A

query
sites
7pn0A

F

L

L

L

V

I

F

F

S

S

G

G

N

Q

S

S

N

N

K

R

T

P

L

L

A

A

Q

Q

G

A

I

I

V

A

A

E

K

A

L

L

G

G

L

L

R

P

L

L

G

G

D

K

A

S

T

T

V

T

S

L

K

R

F

F

S

A

D

N

G

D

E

N

I

L

F

F

V

V

R

R

I

Y

N

E

E

E

S

S

V

L

R

R

G

E

R

G

D

D

V

V

F

F

V

I

V

V

Q

Q

S

S

T

F

N

V

Y

P

P

P

A

V

E

Q

V

D

H

H

L

L

M

M

E

E

L

L

M

L

I

M

M

M

V

V

D

D

A

A

L

A

K

K

R

G

A

A

S

S

A

A

K

A

S

R

V

V

T

T

A

A

V

V

M

I

P

P

Y

Y

F

F

G

S

Y

Y

A

A

R
|
R

Q
x
S

D

D

R

K

T

K

V

D

E

A

P

P

R

R

V

I

P

S

I

I

T

T

A

A

K

R

L

L

V

I

A

A

N

D

L

L

T

Q

K

T

S

A

G

G

I

A

D

D

R

R

I

V

V

L

T

T

M

M

D

T

L

L

H
|
H

A

S

G

P

Q

Q

I

V

Q

H

G

G

F

F

D

K

I

I

P

P

V

V

D

D

N

H

L

L

Y

S

S

A

V

E

P

P

I

V

I

I

A

A

K

N

Y

Y

F

F

R

A

D

T

K

R

G

-

M

V

C

D

G

L

E

E

D

N

Y

A

V

V

S

A

P

P

D

D

A

A

G

G

G

D

V

L

T

K

R

R

A

A

R

S

G

A

F

L

A

A

K

R

A

R

L

L

D

G

T

L

S

P

L

L

A

A

I

F

I

I

D

D

K

K

R

E

R

R

S

V

G

S

P

D

N

T

V

E

A

V

K

R

A

V

M

R

N

M

V

L

I

V

G

G

D

E

V

V

K

E

G

G

K

K

G

T

V

A

I

L

I

I

I

V

D

D

M

E

I

I

D

S

T

T

A

A

G

G

T

S

L

L

V

V

E

E

A

A

A

V

H

E

A

A

V

L

L

M

E

Q

H

A

G

G

A

A

T

K

K

E

V

V

V

Y

A

A

G

A

A

A

S

T

H

H

G

G

I

V

L

Y

S

V

G

G

P

P

A

A

I

L

E

D

R

R

I

I

L

A

K

K

S

S

E

P

L

V

E

K

E

E

V

V

V

A

I

A

T

T

D

D

T

T

I

C

E

-

A

P

S

P

K

K

E

E

K

G

L

-

S

-

F

-

S

P

K

K

L

L

K

R

I

T

L

L

S

T

T

V

S

A

D

P

L

L

F

F

A

A

E

E

A

A

I

I

N

W

R

R

I

I

Y

H

K

R

K

G

E

E

S

S

I

V

S

S

L

L

F

F

binding adenosine-5'-diphosphate: R95 (= R95), S96 (≠ Q96), H129 (= H129)

P60891 Ribose-phosphate pyrophosphokinase 1; PPRibP; Phosphoribosyl pyrophosphate synthase I; PRS-I; EC 2.7.6.1 from Homo sapiens (Human) (see 5 papers)
49% identity, 99% coverage: 5:315/315 of query aligns to 6:316/318 of P60891

query
sites
P60891

V

I

F

F

S

S

G

G

N

S

S

S

N

H

K

Q

T

D

L

L

A
x
S

Q

Q

G

K

I

I

V

A

A

D

K

R

L

L

G

G

L

L

R

E

L

L

G

G

D

K

A

V

T

V

V

T

S

K

K

K

F

F

S

S

D

N

G

Q

E

E

I

T

F

C

V

V

R

E

I

I

N

G

E

E

S

S

V

V

R

R

G

G

R

E

D
|
D

V

V

F

Y

V

I

V

V

Q

Q

S

S

T

G

N

C

Y

G

P

E

A

I

E

N

V

D

H

N

L

L

M

M

E

E

L

L

M

L

I

I

M

M

V

I

D

N

A

A

L

C

K

K

R

I

A

A

S

S

A

A

K

S

S

R

V

V

T

T

A

A

V

V

M

I

P

P

Y

C

F

F

G

P

Y

Y

A

A

R

R

Q

Q

D

D

R

K

T

K

V

D

E

K

P

S

R

R

V

A

P

P

I

I

T

S

A

A

K

K

L

L

V

V

A

A

N
|
N

L

M

L

L

T

S

K

V

S

A

G

G

I

A

D

D

R

H

I

I

V

I

T

T

M

M

D

D

L
|
L

H

H

A

A

G
x
S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V
|
V

D

D

N
|
N

L

L

Y
|
Y

S

A

V

E

P

P

I

A

I

V

A

L

K

K

Y

W

F

I

R

R

D

E

K

N

G

I

M

S

C

E

G

W

E

R

D

N

Y

C

V

T

V

I

V

V

S

S

P

P

D

D

A

A

G

G

V

A

T

K

R

R

A

V

R

T

G

S

F

I

A

A

K
x
D

A

R

L

L

D

N

T

V

S

D

L

F

A
|
A

I

L

I

I

D
x
H

K

K

R

E

R

R

S

K

G

K

P

A

N

N

V

E

A

V

K
x
D

A

R

M

M

N

V

V

L

I

V

G

G

D

D

V

V

K

K

G

D

K

R

G

V

V

A

I

I

I

L

I
x
V

D

D

M

M

I

A

D

D

T

T

A

C

G

G

T

T

L

I

V

C

E
x
H

A

A

H

D

A

K

V

L

L

L

E

S

H

A

G

G

A

A

T

T

K

R

V

V

V

Y

A

A

G

I

A

L

S

T

H

H

G

G

I

I

L

F

S

S

G

G

P

P

A

A

I

I

E

S

R

R

I

I

L

N

K

N

S

A

E

C

L

F

E

E

E

A

V

V

I

V

T

T

D

N

T

T

I

I

E

P

A

Q

S

E

K

D

E

K

K

M

L

K

S

H

F

C

S

S

K

K

L

I

K

Q

I

V

L

I

S

D

T

I

S

S

D

M

L

I

F

L

A

A

E

E

A

A

I

I

N

R

R

R

I

T

Y

H

K

N

K

G

E

E

S

S

I

V

S

S

S

Y

L

L

F

F

V

S

N

H

I

V

S16 (≠ A15) to P: found in patients with phosphoribosyl pyrophosphate synthetase I deficiency; likely pathogenic; dbSNP:rs869025594
D52 (= D51) to H: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852542
N114 (= N113) to S: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852540
L129 (= L128) to I: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852543
S132 (≠ G131) mutation to A: Reduces catalytic activity.; mutation to F: No effect on catalytic activity.
V142 (= V141) to L: found in a patient with an intermediate phenotype between ARTS and PRPS1 superactivity; likely pathogenic; normal PRPP synthetase activity in fibroblasts; loss of activity in erythrocytes; dbSNP:rs398122855
N144 (= N143) mutation to H: No effect on catalytic activity.
Y146 (= Y145) mutation to F: No effect on catalytic activity.; mutation to M: Reduces catalytic activity.
D183 (≠ K182) to H: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852541
A190 (= A189) to V: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852544
H193 (≠ D192) to Q: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852545
D203 (≠ K202) to H: in a breast cancer sample; somatic mutation
V219 (≠ I218) to G: in a breast cancer sample; somatic mutation
H231 (≠ E230) to D: in a colorectal cancer sample; somatic mutation

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

8dbkB Human prps1 with phosphate, atp, and r5p; hexamer with resolved catalytic loops (see paper)
49% identity, 99% coverage: 5:315/315 of query aligns to 5:315/316 of 8dbkB

query
sites
8dbkB

V

I

F

F

S

S

G

G

N

S

S

S

N

H

K

Q

T

D

L

L

A

S

Q

Q

G

K

I

I

V

A

A

D

K

R

L

L

G

G

L

L

R

E

L

L

G

G

D

K

A

V

T

V

V

T

S

K

K

K

F
|
F

S

S

D
x
N

G

Q

E
|
E

I

T

F

C

V

V

R

E

I

I

N

G

E

E

S
|
S

V

V

R
|
R

G

G

R

E

D

D

V

V

F

Y

V

I

V

V

Q

Q

S

S

T

G

N

C

Y

G

P

E

A

I

E

N

V

D

H

N

L

L

M

M

E

E

L

L

M

L

I

I

M

M

V

I

D

N

A

A

L

C

K

K

R

I

A

A

S

S

A

A

K

S

S

R

V

V

T

T

A

A

V

V

M

I

P

P

Y

C

F

F

G

P

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R

K

T

K

V

D

E

K

P

S

R

R

V

A

P

P

I

I

T

S

A

A

K

K

L

L

V

V

A

A

N

N

L

M

L

L

T

S

K

V

S

A

G

G

I

A

D

D

R

H

I

I

V

I

T

T

M

M

D

D

L

L

H
|
H

A

A

G

S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

V

D

D

N

N

L

L

Y

Y

S

A

V

E

P

P

I

A

I

V

A

L

K

K

Y

W

F

I

R

R

D

E

K

N

G

I

M

S

C

E

G

W

E

R

D

N

Y

C

V

T

V

I

V

V

S

S

P

P

D
|
D

A

A

G
|
G

G

G

V

A

T

K

R

R

A

V

R

T

G

S

F

I

A

A

K

D

A

R

L

L

D

N

T

V

S

D

L

F

A

A

I

L

I

I

D

H

K
|
K

R

E

R
|
R

S

K

G

K

P

A

N
|
N

V

E

A

V

K

D

A

R

M

M

N

V

V

L

I

V

G

G

D

D

V

V

K

K

G

D

K

R

G

V

V

A

I

I

I

L

I

V

D
|
D

M

M

I

A

D
|
D

T
|
T

A
x
C

G
|
G

T
|
T

L

I

V

C

E

H

A

A

H

D

A

K

V

L

L

L

E

S

H

A

G

G

A

A

T

T

K

R

V

V

V

Y

A

A

G

I

A

L

S

T

H

H

G

G

I

I

L

F

S

S

G

G

P

P

A

A

I

I

E

S

R

R

I

I

L

N

K

N

S

A

E

C

L

F

E

E

E

A

V

V

I

V

T

T

D

N

T

T

I

I

E

P

A

Q

S

E

K

D

E

K

K

M

L

K

S

H

F

C

S

S

K

K

L

I

K

Q

I

V

L

I

S

D

T

I

S

S

D

M

L

I

F

L

A

A

E

E

A

A

I

I

N

R

R

R

I

T

Y

H

K

N

K

G

E

E

S

S

I

V

S

S

S

Y

L

L

F

F

V

S

N

H

I

V

binding adenosine monophosphate: R95 (= R95), Q96 (= Q96), N199 (= N199)
binding adenosine-5'-triphosphate: F34 (= F34), N36 (≠ D36), E38 (= E38)
binding phosphate ion: S46 (= S46), R48 (= R48)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: H129 (= H129), D170 (= D170), G172 (= G172), K193 (= K193), R195 (= R195), D219 (= D219), D220 (= D220), D223 (= D223), T224 (= T224), C225 (≠ A225), G226 (= G226), T227 (= T227)

8dbeA Human prps1 with adp; hexamer (see paper)
49% identity, 99% coverage: 5:315/315 of query aligns to 5:315/316 of 8dbeA

query
sites
8dbeA

V

I

F

F

S

S

G

G

N

S

S

S

N

H

K

Q

T

D

L

L

A

S

Q

Q

G

K

I

I

V

A

A

D

K

R

L

L

G

G

L

L

R

E

L

L

G

G

D

K

A

V

T

V

V

T

S

K

K

K

F
|
F

S

S

D
x
N

G

Q

E
|
E

I

T

F

C

V

V

R

E

I

I

N

G

E

E

S

S

V

V

R

R

G

G

R

E

D

D

V

V

F

Y

V

I

V

V

Q

Q

S

S

T

G

N

C

Y

G

P

E

A

I

E

N

V

D

H

N

L

L

M

M

E

E

L

L

M

L

I

I

M

M

V

I

D

N

A

A

L

C

K

K

R

I

A

A

S

S

A

A

K

S

S

R

V

V

T

T

A

A

V

V

M

I

P

P

Y

C

F

F

G

P

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
x
K

T
x
K

V
x
D

E

K

P
x
S

R
|
R

V

A

P

P

I

I

T

S

A

A

K

K

L

L

V

V

A

A

N

N

L

M

L

L

T

S

K

V

S

A

G

G

I

A

D

D

R

H

I

I

V

I

T

T

M

M

D

D

L

L

H
|
H

A

A

G

S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

V

D
|
D

N

N

L

L

Y
|
Y

S

A

V

E

P

P

I

A

I

V

A

L

K

K

Y

W

F

I

R

R

D

E

K

N

G

I

M

S

C

E

G

W

E

R

D

N

Y

C

V

T

V

I

V

V

S

S

P

P

D
|
D

A

A

G

G

V

A

T

K

R

R

A

V

R

T

G

S

F

I

A

A

K

D

A

R

L

L

D

N

T

V

S

D

L

F

A

A

I

L

I

I

D

H

K

K

R

E

R

R

S

K

G

K

P

A

N

N

V

E

A

V

K

D

A

R

M

M

N

V

V

L

I

V

G

G

D

D

V

V

K

K

G

D

K

R

G

V

V

A

I

I

I

L

I

V

D
|
D

M

M

I

A

D
|
D

T
|
T

A

C

G
|
G

T
|
T

L

I

V

C

E

H

A

A

H

D

A

K

V

L

L

L

E

S

H

A

G

G

A

A

T

T

K

R

V

V

V

Y

A

A

G

I

A

L

S

T

H

H

G

G

I

I

L

F

S

S

G

G

P

P

A

A

I

I

E

S

R

R

I

I

L

N

K

N

S

A

E

C

L

F

E

E

E

A

V

V

I

V

T

T

D

N

T

T

I

I

E

P

A

Q

S

E

K

D

E

K

K

M

L

K

S

H

F

C

S

S

K

K

L

I

K

Q

I

V

L

I

S

D

T

I

S

S

D

M

L

I

F

L

A

A

E

E

A

A

I

I

N

R

R

R

I

T

Y

H

K

N

K

G

E

E

S
|
S

I
x
V

S
|
S

S

Y

L

L

F
|
F

V

S

N

H

I

V

binding adenosine-5'-diphosphate: F34 (= F34), N36 (≠ D36), E38 (= E38), R95 (= R95), Q96 (= Q96), K98 (≠ R98), K99 (≠ T99), D100 (≠ V100), S102 (≠ P102), R103 (= R103), H129 (= H129), D142 (= D142), Y145 (= Y145), S307 (= S307), V308 (≠ I308), S309 (= S309), F312 (= F312)
binding 5-O-phosphono-alpha-D-ribofuranose: H129 (= H129), D170 (= D170), D219 (= D219), D220 (= D220), D223 (= D223), T224 (= T224), G226 (= G226), T227 (= T227)

2hcrA Crystal structure of human phosphoribosyl pyrophosphate synthetase 1 in complex with amp(atp), cadmium and sulfate ion (see paper)
49% identity, 98% coverage: 5:312/315 of query aligns to 4:305/305 of 2hcrA

query
sites
2hcrA

V

I

F

F

S

S

G

G

N

S

S

S

N

H

K

Q

T

D

L

L

A

S

Q

Q

G

K

I

I

V

A

A

D

K

R

L

L

G

G

L

L

R

E

L

L

G

G

D

K

A

V

T

V

V

T

S

K

K

K

F

F

S

S

D

N

G

Q

E

E

I

T

F

C

V

V

R

E

I

I

N

G

E

E

S

S

V

V

R

R

G

G

R

E

D

D

V

V

F

Y

V

I

V

V

Q

Q

S

S

T

G

N

C

Y

G

P

E

A

I

E

N

V

D

H

N

L

L

M

M

E

E

L

L

M

L

I

I

M

M

V

I

D

N

A

A

L

C

K

K

R

I

A

A

S

S

A

A

K

S

S

R

V

V

T

T

A

A

V

V

M

I

P

P

Y

C

F

F

G

P

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
x
K

T

K

V

D

E

K

P

S

R

R

V

A

P

P

I

I

T

S

A

A

K

K

L

L

V

V

A

A

N

N

L

M

L

L

T

S

K

V

S

A

G

G

I

A

D

D

R

H

I

I

V

I

T

T

M

M

D

D

L

L

H
|
H

A

A

G

S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

V

D

D

N

N

L

L

Y

Y

S

A

V

E

P

P

I

A

I

V

A

L

K

K

Y

W

F

I

R

R

D

E

K

N

G

I

M

S

C

E

G

W

E

R

D

N

Y

C

V

T

V

I

V

V

S

S

P

P

D

D

A

A

G

G

V

A

T

K

R

R

A

V

R

T

G

S

F

I

A

A

K

D

A

R

L

L

D

N

T

V

S

D

L

F

A

A

I

L

I

I

D

H

K

K

R

E

R

R

S

D

G

-

P

-

N

-

V

-

A

-

K

-

A

R

M

M

N

V

V

L

I

V

G

G

D

D

V

V

K

K

G

D

K

R

G

V

V

A

I

I

I

L

I

V

D

D

M

M

I

A

D

D

T

T

A

C

G

G

T

T

L

I

V

C

E

H

A

A

H

D

A

K

V

L

L

L

E

S

H

A

G

G

A

A

T

T

K

R

V

V

V

Y

A

A

G

I

A

L

S

T

H

H

G

G

I

I

L

F

S

S

G

G

P

P

A

A

I

I

E

S

R

R

I

I

L

N

K

N

S

A

E

C

L

F

E

E

E

A

V

V

I

V

T

T

D

N

T

T

I

I

E

P

A

Q

S

E

K

D

E

K

K

M

L

K

S

H

F

C

S

S

K

K

L

I

K

Q

I

V

L

I

S

D

T

I

S

S

D

M

L

I

F

L

A

A

E

E

A

A

I

I

N

R

R

R

I

T

Y

H

K

N

K

G

E

E

S

S

I

V

S

S

S

Y

L

L

F

F

binding adenosine monophosphate: R94 (= R95), Q95 (= Q96), K97 (≠ R98), H128 (= H129)

8dbgA Human prps1 with phosphate and atp; hexamer (see paper)
48% identity, 99% coverage: 5:315/315 of query aligns to 5:308/309 of 8dbgA

query
sites
8dbgA

V

I

F

F

S

S

G

G

N

S

S

S

N

H

K

Q

T

D

L

L

A

S

Q

Q

G

K

I

I

V

A

A

D

K

R

L

L

G

G

L

L

R

E

L

L

G

G

D

K

A

V

T

V

V

T

S

K

K

K

F
|
F

S

S

D
x
N

G

Q

E
|
E

I

T

F

C

V

V

R

E

I

I

N

G

E

E

S
|
S

V

V

R
|
R

G

G

R

E

D

D

V

V

F

Y

V

I

V

V

Q

Q

S

S

T

G

N

C

Y

G

P

E

A

I

E

N

V

D

H

N

L

L

M

M

E

E

L

L

M

L

I

I

M

M

V

I

D

N

A

A

L

C

K

K

R

I

A

A

S

S

A

A

K

S

S

R

V

V

T

T

A

A

V

V

M

I

P

P

Y

C

F

F

G

P

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
x
K

T

K

V

D

E

K

P

S

R

R

V

A

P

P

I

I

T

S

A

A

K

K

L

L

V

V

A

A

N

N

L

M

L

L

T

S

K

V

S

A

G

G

I

A

D

D

R

H

I

I

V

I

T

T

M

M

D

D

L

L

H
|
H

A

A

G

S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

V

D

D

N

N

L

L

Y

Y

S

A

V

E

P

P

I

A

I

V

A

L

K

K

Y

W

F

I

R

R

D

E

K

N

G

I

M

S

C

E

G

W

E

R

D

N

Y

C

V

T

V

I

V

V

S

S

P

P

D

D

A

A

G

G

V

A

T

K

R

R

A

V

R

T

G

S

F

I

A

A

K

D

A

R

L

L

D

N

T

V

S

D

L

F

A

A

I

L

I

I

D

H

K

K

R

E

R

-

S

-

G

-

P

-

N

-

V

-

A

-

K

D

A

R

M

M

N

V

V

L

I

V

G

G

D

D

V

V

K

K

G

D

K

R

G

V

V

A

I

I

I

L

I

V

D

D

M

M

I

A

D
|
D

T
|
T

A
x
C

G

G

T
|
T

L

I

V

C

E

H

A

A

H

D

A

K

V

L

L

L

E

S

H

A

G

G

A

A

T

T

K

R

V

V

V

Y

A

A

G

I

A

L

S

T

H

H

G

G

I

I

L

F

S

S

G

G

P

P

A

A

I

I

E

S

R

R

I

I

L

N

K

N

S

A

E

C

L

F

E

E

E

A

V

V

I

V

T

T

D

N

T

T

I

I

E

P

A

Q

S

E

K

D

E

K

K

M

L

K

S

H

F

C

S

S

K

K

L

I

K

Q

I

V

L

I

S

D

T

I

S

S

D

M

L

I

F

L

A

A

E

E

A

A

I

I

N

R

R

R

I

T

Y

H

K

N

K

G

E

E

S

S

I

V

S

S

S

Y

L

L

F

F

V

S

N

H

I

V

binding adenosine-5'-triphosphate: F34 (= F34), N36 (≠ D36), E38 (= E38), R95 (= R95), Q96 (= Q96), K98 (≠ R98), H129 (= H129)
binding phosphate ion: S46 (= S46), R48 (= R48), D216 (= D223), T217 (= T224), C218 (≠ A225), T220 (= T227)

Query Sequence

>WP_013450369.1 NCBI__GCF_000183405.1:WP_013450369.1
MDFLVFSGNSNKTLAQGIVAKLGLRLGDATVSKFSDGEIFVRINESVRGRDVFVVQSTNY
PAEVHLMELMIMVDALKRASAKSVTAVMPYFGYARQDRTVEPRVPITAKLVANLLTKSGI
DRIVTMDLHAGQIQGFFDIPVDNLYSVPIIAKYFRDKGMCGEDYVVVSPDAGGVTRARGF
AKALDTSLAIIDKRRSGPNVAKAMNVIGDVKGKGVIIIDDMIDTAGTLVEAAHAVLEHGA
TKVVAGASHGILSGPAIERILKSELEEVVITDTIEASKEKLSFSKLKILSTSDLFAEAIN
RIYKKESISSLFVNI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory