SitesBLAST

Q9X0C6 Imidazole glycerol phosphate synthase subunit HisF; IGP synthase cyclase subunit; IGP synthase subunit HisF; ImGP synthase subunit HisF; IGPS subunit HisF; EC 4.3.2.10 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
56% identity, 100% coverage: 1:253/253 of query aligns to 1:250/253 of Q9X0C6

query
sites
Q9X0C6

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L

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V

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K

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D

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T

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N

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C9 (= C9) mutation to A: No change in activity.
D11 (= D11) mutation to X: Loss of activity.
K19 (= K19) mutation to S: Decrease in activity.
D51 (= D51) mutation to N: No change in activity.
N103 (= N103) mutation to A: No change in activity.
D130 (= D130) mutation D->A,C,F,G,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y: Complete loss of activity.; mutation to E: Weak activity.
D176 (= D178) mutation to N: Decrease in activity.
D183 (= D185) mutation to N: No change in activity.

7ac8A Molecular basis for the unique allosteric activation mechanism of the heterodimeric imidazole glycerol phosphate synthase complex. (see paper)
56% identity, 100% coverage: 1:253/253 of query aligns to 1:250/252 of 7ac8A

query
sites
7ac8A

M

M

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S

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M

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S

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active site: D11 (= D11), D130 (= D130)
binding [(2R,3S,4R,5R)-5-[4-aminocarbonyl-5-[(E)-[[(2R,3R,4S,5R)-3,4-bis(oxidanyl)-5-(phosphonooxymethyl)oxolan-2-yl]amino]methylideneamino]imidazol-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl dihydrogen phosphate: L50 (= L50), I52 (= I52), G82 (= G82), N103 (= N103), T104 (= T104), D130 (= D130), S144 (≠ G146), D176 (= D178), G177 (= G179), S201 (= S203), S225 (= S228)

1gpwC Structural evidence for ammonia tunneling across the (beta/alpha)8 barrel of the imidazole glycerol phosphate synthase bienzyme complex. (see paper)
55% identity, 100% coverage: 1:253/253 of query aligns to 1:250/253 of 1gpwC

query
sites
1gpwC

M

M

L

L

A

A

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K

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C

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D
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N

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V

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K

D

D

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G

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R

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K

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N

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M

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G

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L

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I

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L

S

R

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G

A

A

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D

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K

V

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S

S

I

I

N
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N

T
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T

A

A

V

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K

E

N

N

P

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S

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T

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I

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A

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A

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D

A

A

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K

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K

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T

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N

D

G

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S

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G

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W

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E

M

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Y

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T

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Y

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G

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A

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K

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M

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S

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G

G

A

A

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G

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E

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I

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L

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S

M

I

D

D

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R

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D

G
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G

T

T

K

K

D

S

G

G

Y

Y

D

D

L

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E

E

L

M

T

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F

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T

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S

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K

M

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L

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F

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L

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A

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G

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A

D

D

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A

L

L

A

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S
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S

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F

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H

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L

active site: N11 (≠ D11), D130 (= D130)
binding phosphate ion: G82 (= G82), N103 (= N103), T104 (= T104), D176 (= D178), G177 (= G179), S225 (= S228)

7qc8A Imidazole glycerol phosphate synthase subunit HisF (see paper)
54% identity, 100% coverage: 2:253/253 of query aligns to 2:250/250 of 7qc8A

query
sites
7qc8A

L

L

A

A

K

K

R

R

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I

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A

C

A

L

L

D
x
E

V

V

K

K

D

D

G

G

R

R

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V

K

K

G

G

T

T

Q

N

F

F

L

E

N

N

L

L

I

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D

D

A

S

G

G

D

D

P

P

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A

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E

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L

A

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K

E

F

Y

Y

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S

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E

Q

I

G

G

A

I

D

D

E

E

L

L

T

V

F

F

L
x
H

D

D

I
x
H

T

T

A

A

S

S

H

V

E

E

N

K

R

R

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K

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L

L

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N

L

S

R

G

G

A

A

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D

K

K

V

V

S

S

I

I

N

N

T

T

A

A

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E

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N

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L

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T

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A

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A

A

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C

A

I

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A

A

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I

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D

A

A

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K

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R

K

-

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N

D

G

G

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E

G

-

W

F

E

M

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V

Y

F

T

T

H

Y

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T

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G

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W

A

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A

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T

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S

M

I

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D

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R

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D

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T

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K

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Y

D

D

L

T

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E

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T

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A

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I

I

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A

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S

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G

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A

G

G

T

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K

M

E

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D

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I

F

L

L

D

E

G

A

F

-

T

F

K

L

G

A

K

G

A

A

D

D

A

A

L

L

A

A

S

S

I

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F

F

H

H

F

F

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K

D

I

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F

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E

E

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E

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Y

L

L

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K

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K

N

H

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G

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R

L

L

binding zinc ion: E11 (≠ D11), H50 (≠ L50), H52 (≠ I52)

3zr4E Structural evidence for ammonia tunneling across the (beta-alpha)8 barrel of the imidazole glycerol phosphate synthase bienzyme complex (see paper)
53% identity, 100% coverage: 1:253/253 of query aligns to 1:241/244 of 3zr4E

query
sites
3zr4E

M

M

L

L

A

A

K

K

R

R

I

I

P

A

C

C

L

L

D
|
D

V

V

K

K

D

D

G

G

R

R

V

V

K

K

G

-

T

-

Q

-

F

-

L

-

N

-

L

-

I

-

D

-

A

S

G

G

D

D

P

P

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V

Q

E

V

L

A

G

E

K

E

F

Y

Y

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I

G

G

A

I

D

D

E

E

L

L

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F

L

L

D

D

I

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T

A

A

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S

H

V

E

E

N

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E

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L

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M

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F

E

E

D

T

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K

E

L

L

L

I

N

L

S

R

G

G

A

A

D

D

K

K

V

V

S

S

I

I

N

N

T

T

A

A

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K

E

N

N

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P

D

S

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T

D

Q

A

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A

A

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T

F

F

G

G

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S

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Q

C

A

I

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V

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A

A

I

I

D
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D

A

A

K

K

R

R

K

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T

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N

D

G

G

S

E

G

-

W

F

E

M

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V

Y

F

T

T

H

Y

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K

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N

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T

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W

A

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A

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S

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D

D

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T

K

K

D

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G

G

Y

Y

D

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L

T

E

E

L

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T

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A
x
R

A

F

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A

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I

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I

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P

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I

I

I

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A

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S

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A

G

G

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K

K

M

E

E

D

H

I

F

L

L

D

E

G

A

F

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T

F

K
x
L

G

A

K

G

A

A

D

D

A

A

L

L

A

A

S

S

I

V

F

F

H

H

F

F

G

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I

E

Y

I

K

D

I

V

F

R

E

E

I

L

K

K

Q

E

F

Y

L

L

K

K

E

K

N

H

G

G

V

V

Q

N

V

V

R

R

L

L

active site: D11 (= D11), D121 (= D130)
binding glycerol: R179 (≠ A190), L206 (≠ K218)

5d2tA Directed evolutionary changes in kemp eliminase ke07 - crystal 3 wild type
51% identity, 99% coverage: 3:253/253 of query aligns to 1:248/251 of 5d2tA

query
sites
5d2tA

A

A

K

K

R

R

I

I

P

A

C

A

L

L

D

I

V

V

K

K

D

D

G

G

R

R

V

V

K

K

G

G

T

S

Q

N

F

F

L

E

N

N

L

L

I

R

D

D

A

S

G

G

D

D

P

P

V

V

Q

E

V

L

A

G

E

K

E

F

Y

Y

D

S

R

E

Q

I

G

G

A

I

D

D

E

E

L

L

T

S

F

F

L
x
W

D

D

I

I

T

T

A

A

S

S

H

V

E

E

N

K

R

R

G

K

I

T

I

M

L

L

D

E

V

L

V

V

A

E

K

K

T

V

A

A

E

E

K

Q

V

I

F

D

M

I

P

P

L

F

T

T

V

V

G

G

I

I

R

H

T

D

I

F

E

E

D

T

I

A

R

S

K

E

L

L

L

I

N

L

S

R

G

G

A

A

D

D

K

K

V

V

S
x
E

I

I

N

N

T

T

A

A

V

V

K

E

N

N

P

P

D

S

F

L

V

I

K

T

D

Q

A

I

A

A

L

Q

R

T

F

F

G

G

S

S

Q

Q

C

A

I

V

V

V

A
x
Y

I

I

D

A

A

A

K

K

R

R

K

-

T

V

N

D

G

G

S

E

G

-

W

F

E

M

V

V

Y

F

T

T

H

Y

G

S

G

G

R

K

N

K

P

N

T

T

G

G

I

I

D

L

V

L

L

R

E

D

W

W

A

V

V

V

K

E

M

V

E

E

S

K

F

R

G

G

A

A

G

G

E

E

I

I

L

V

L

L

T

G

S

S

M

I

D

D

K

R

D

L

G

G

T

T

K

K

D

S

G

G

Y

Y

D

D

L

T

E

E

L

M

T

I

A

R

A

F

V

V

S

R

D

P

A

L

I

T

R

T

I

L

P

P

V

I

I

I

A

A

S

H

G

G

V

A

G

G

T

K

K

M

E

E

D

H

I

F

L

L

D

E

G

A

F

F

T

L

K

A

G

G

K

-

A

A

D

D

A

A

L
x
K

A

A

A

N

S

S

I

V

F

L

H

H

F

F

G

R

I

E

Y

I

K

D

I

V

F

R

E

E

I

L

K

K

Q

E

F

Y

L

L

K

K

E

K

N

H

G

G

V

V

Q

N

V

V

R

R

L

L

binding 5-nitro-2-oxidanyl-benzenecarbonitrile: W48 (≠ L50), E99 (≠ S101), Y126 (≠ A128), K220 (≠ L225)

4ewnD Structure of hisf-d130v+d176v with bound rcdrp (see paper)
53% identity, 100% coverage: 2:253/253 of query aligns to 1:243/243 of 4ewnD

query
sites
4ewnD

L

L

A

A

K

K

R

R

I

I

P

A

C

C

L

L

D

D

V

V

K

K

D

D

G

G

R

R

V

V

K

K

G

G

T

T

Q

N

F

F

L

E

N

N

L

L

I

R

D

D

A

S

G

G

D

D

P

P

V

V

Q

E

V

L

A

G

E

K

E

F

Y

Y

D

S

R

E

Q

I

G

G

A

I

D

D

E

E

L

L

T

V

F

F

L

L

D

D

I

I

T

T

A

K

S

T

H

-

E

-

N

-

R

-

G

-

I

-

I

M

L

L

D

E

V

L

V

V

A

E

K

K

T

V

A

A

E

E

K

Q

V

I

F

D

M

I

P

P

L

F

T

T

V

V

G

G

I

I

R

H

T

D

I

F

E

E

D

T

I

A

R

S

K

E

L

L

L

I

N

L

S

R

G

G

A

A

D

D

K

K

V

V

S

S

I

I

N

N

T

T

A

A

V

V

K

E

N

N

P

P

D

S

F

L

V

I

K

T

D

Q

A

I

A

A

L

Q

R

T

F

F

G

G

S

S

Q

Q

C

A

I

V

V

V

A

A

I

I

D
x
V

A

A

K

K

R

R

K

-

T

V

N

D

G

G

S

E

G

-

W

F

E

M

V

V

Y

F

T

T

H

Y

G

S

G

G

R

K

N

K

P

N

T

T

G

G

I

I

D

L

V

L

L

R

E

D

W

W

A

V

V

V

K

E

M

V

E

E

S

K

F

R

G

G

A

A

G

G

E

E

I

I

L

L

T

T

S

S

M

I

D

D

K

R

D

V

G
|
G

T

T

K

K

D

S

G

G

Y

Y

D

D

L

T

E

E

L

M

T

I

A

R

A

F

V

V

S

R

D

P

A

L

I

T

R

T

I

L

P

P

V

I

I

I

A

A

S
|
S

G
|
G

V

A

G

G

T

K

K

M

E

E

D

H

I

F

L

L

D

E

G

A

F

-

T

F

K

L

G

A

K

G

A

A

D

D

A

A

L
|
L

A
|
A

S
|
S

I

V

F

F

H

H

F

F

G

R

I

E

Y

I

K

D

I

V

F

R

E

E

I

L

K

K

Q

E

F

Y

L

L

K

K

E

K

N

H

G

G

V

V

Q

N

V

V

R

R

L

L

binding 1-(O-carboxy-phenylamino)-1-deoxy-D-ribulose-5-phosphate: V123 (≠ D130), G170 (= G179), S194 (= S203), G195 (= G204), G196 (= G205), L215 (= L225), A216 (= A226), A217 (= A227), S218 (= S228)

6dnjA Directed evolutionary changes in kemp eliminase ke07 - crystal 28 round 5 (see paper)
50% identity, 100% coverage: 2:253/253 of query aligns to 1:249/250 of 6dnjA

query
sites
6dnjA

L

L

A

A

K

K

R

R

I

I

I

D

P

A

C

A

L

L

D

I

V

M

K

K

D

D

G

G

R

R

V

V

K

K

G

G

T

S

Q

N

F

F

L

E

N

N

L

L

I

R

D

D

A

S

G

G

D

D

P

P

V

V

Q

E

V

L

A

G

E

K

E

F

Y

Y

D

S

R

E

Q

I

G

G

A

I

D

D

E

E

L

L

T

S

F

F

L
x
W

D

D

I

I

T

T

A

A

S

S

H

V

E

E

N

K

R

R

G

K

I

T

I

M

L

L

D

E

V

L

V

V

A

E

K

K

T

V

A

A

E

E

K

Q

V

I

F

D

M

I

P

P

L

F

T

T

V

V

G
|
G

G

G

I

I

R

H

T

D

I

F

E

E

D

T

I

A

R

S

K

E

L

L

L

I

N

L

S

R

G

G

A

A

D

D

K

K

V

V

S

E

I

I

N
|
N

T

T

A

A

V

V

K

E

N

N

P

P

D

S

F

L

V

I

K

T

D

Q

A

I

A

A

L

Q

R

T

F

F

G

G

S

S

Q

Q

C

A

I

V

V

V

A
x
Y

I

I

D

A

A

A

K

K

R

R

K

-

T

V

N

D

G

G

S

E

G

-

W

F

E

M

V

V

Y

F

T

T

H

Y

G

S

G

G

R

K

N

K

P

N

T

T

G

G

I

I

D

L

V

L

L

R

E

D

W

W

A

V

V

V

K

E

M

V

E

E

S

K

F

R

G

G

A

A

G

G

E

E

I

I

L

V

L

L

T

G

S

S

M

I

D

D

K

R

D

L

G

G

T

T

K

K

D

S

G

G

Y

Y

D

D

L

T

E

E

L

M

T

I

A

R

A

F

V

V

S

R

D

P

A

L

I

T

R

T

I

L

P

P

V

I

I

I

A

A

S

H

G

R

G

G

V

A

G

G

T

K

K

M

E

E

D

H

I

F

L

L

D

E

G

A

F

F

T

L

K

A

G

G

K

-

A

A

D

D

A

A

L

K

A

A

A

D

S

S

I

V

F

F

H

H

F

F

G

R

I

E

Y

I

K

D

I

V

F

R

E

E

I

L

K

K

Q

E

F

Y

L

L

K

K

E

K

N

H

G

G

V

V

Q

N

V

V

R

R

L

L

binding 5-nitro-1,2-benzoxazole: W49 (≠ L50), G79 (= G80), G80 (= G81), N102 (= N103), Y127 (≠ A128)

P60664 Imidazole glycerol phosphate synthase subunit HisF; IGP synthase cyclase subunit; IGP synthase subunit HisF; ImGP synthase subunit HisF; IGPS subunit HisF; EC 4.3.2.10 from Escherichia coli (strain K12) (see paper)
46% identity, 100% coverage: 1:253/253 of query aligns to 1:257/258 of P60664

query
sites
P60664

M

M

L

L

A

A

K

K

R
|
R

I

I

P

P

C

C

L

L

D

D

V

V

K

R

D

D

G

G

R

Q

V

V

K

K

G

G

T

V

Q

Q

F

F

L

R

N

N

L

H

I

E

D

I

A

I

G

G

D

D

P

I

V

V

Q

P

V

L

A

A

E

K

E

R

Y

Y

D

A

R

E

Q

E

G

G

A

A

D

D

E
|
E

L

L

T

V

F

F

L

Y

D

D

I

I

T

T

A

A

S

S

H

S

E

D

N

G

R

R

G

V

I

V

I

D

L

K

D

S

V

W

V

V

A

S

K

R

T

V

A

A

E

E

K

V

V

I

F

D

M

I

P

P

L

F

T

C

V

V

G

A

G

G

I

I

R

K

T

S

I

L

E

E

D

D

I

A

R

A

K

K

L

I

L

L

N

S

S

F

G

G

A

A

D

D

K

K

V

I

S

S

I

I

N

N

T

S

A

P

A

A

V

L

K

A

N

D

P

P

D

T

F

L

V

I

K

T

D

R

A

L

A

A

L

D

R

R

F

F

G

G

S

V

Q
|
Q

C
|
C

I

I

V

V

A

G

I

I

D

D

A

T

K

W

R

Y

K

D

T

A

N

E

G

T

S

G

G

K

W

Y

E

H

V

V

Y

N

T

Q

H

Y

G

T

G

G

R

D

N

E

P

S

-

R

-

T

-

R

-

V

T

T

G

Q

I

W

D

E

V

T

L

L

E

D

W

W

A

V

V

Q

K

E

M

V

E

Q

S

K

F

R

G

G

A

A

G

G

E

E

I

I

L

V

L

L

T

N

S

M

M

M

D

N

K

Q

D

D

G

G

T

V

K

R

D

N

G

G

Y

Y

D

D

L

L

E

E

L

Q

T

L

A

K

V

V

S

R

D

E

A

V

I

C

R

H

I

V

P

P

V

L

I

I

A

A

S

S

G

G

V

A

G

G

T

T

K

M

E

E

D

H

I

F

L

L

D

E

G

A

F

F

T

R

K

D

G

A

K

D

A

V

D

D

A

G

A

A

L

L

A

A

S

S

I

V

F

F

H

H

F

K

G

Q

I

I

Y

I

K

N

I

I

F

G

E

E

I

L

K

K

Q

A

F

Y

L

L

K

A

E

T

N

Q

G

G

V

V

Q

E

V

I

R

R

L

I

R5 (= R5) mutation to A: Loss of activity.; mutation to H: Loss of IGP synthase activity. Weak IGP synthase activity and reduced HisH activity in vitro.
E46 (= E46) mutation to A: Loss of activity.; mutation to G: Loss of IGP synthase activity. Weak IGP synthase and HisH activities in vitro.
Q123 (= Q123) mutation to A: Decrease in activity.; mutation to R: Loss of IGP synthase activity. Weak HisH activity in vitro.
C124 (= C124) mutation to A: No change in activity.; mutation to R: Loss of IGP synthase activity. Weak HisH activity in vitro.

2wjzE Crystal structure of (hish) k181a y138a mutant of imidazoleglycerolphosphate synthase (hish hisf) which displays constitutive glutaminase activity (see paper)
52% identity, 100% coverage: 1:253/253 of query aligns to 1:236/237 of 2wjzE

query
sites
2wjzE

M

M

L

L

A

A

K

K

R

R

I

I

P

A

C

C

L

L

D
|
D

V

V

K

K

D

D

G

G

R

R

V

V

K

-

G

-

T

-

Q

-

F

-

L

-

N

-

L

-

I

-

D

-

A

-

G

G

D

D

P

P

V

V

Q

E

V

L

A

G

E

K

E

F

Y

Y

D

S

R

E

Q

I

G

G

A

I

D

D

E

E

L

L

T

V

F

F

L

L

D

D

I

I

T

T

A

A

S

-

H

-

E

-

N

K

R

R

G

K

I

T

I

M

L

L

D

E

V

L

V

V

A

E

K

K

T

V

A

A

E

E

K

Q

V

I

F

D

M

I

P

P

L

F

T

T

V

V

G

G

G
|
G

I

I

R

H

T

D

I

F

E

E

D

T

I

A

R

S

K

E

L

L

L

I

N

L

S

R

G

G

A

A

D

D

K

K

V

V

S

S

I

I

N
|
N

T
|
T

A

A

V

V

K

E

N

N

P

P

D

S

F

L

V

I

K

T

D

Q

A

I

A

A

L

Q

R

T

F

F

G

G

S

S

Q

Q

C

A

I

V

V

V

A

A

I

I

D
|
D

A

A

K

K

R

R

K

-

T

V

N

D

G

G

S

E

G

-

W

F

E

M

V

V

Y

F

T

T

H

Y

G

S

G

G

R

K

N

K

P

N

T

T

G

G

I

I

D

L

V

L

L

R

E

D

W

W

A

V

V

V

K

E

M

V

E

E

S

K

F

R

G

G

A

A

G

G

E

E

I

I

L

L

T

T

S

S

M

I

D

D

K

R

D

D

G

G

T

T

K

K

D

S

G

G

Y

Y

D

D

L

T

E

E

L

M

T

I

A

R

A

F

V

V

S

R

D

P

A

L

I

T

R

T

I

L

P

P

V

I

I

I

A

A

S

S

G

G

V

A

G

G

T

K

K

M

E

E

D

H

I

F

L

L

D

E

G

A

F

-

T

F

K

L

G

A

K

G

A

A

D

D

A

A

L

L

A

A

S

S

I

V

F

F

H

H

F

F

G

R

I

E

Y

I

K

D

I

V

F

R

E

E

I

L

K

K

Q

E

F

Y

L

L

K

K

E

K

N

H

G

G

V

V

Q

N

V

V

R

R

L

L

active site: D11 (= D11), D116 (= D130)
binding phosphate ion: G68 (= G82), N89 (= N103), T90 (= T104)

3iivB Evolutionary optimization of computationally designed enzymes: kemp eliminases of the ke07 series (see paper)
49% identity, 100% coverage: 2:253/253 of query aligns to 2:249/262 of 3iivB

query
sites
3iivB

L

L

A

A

K

K

R

R

I

I

I

D

P

A

C

A

L

L

D

I

V

L

K

K

D

D

G

G

R

R

V

V

K

K

G

G

T

S

Q

N

F

F

L

E

N

N

L

L

I

R

D

D

A

S

G

G

D

D

P

P

V

V

Q

E

V

L

A

G

E

K

E

F

Y

Y

D

S

R

E

Q

I

G

G

A

I

D

D

E

E

L

L

T

S

F

F

L

W

D

D

I

I

T

T

A

A

S

S

H

V

E

E

N

K

R

K

G

-

I

T

I

M

L

L

D

E

V

L

V

V

A

E

K

K

T

V

A

A

E

E

K

Q

V

I

F

D

M

I

P

P

L

I

T

T

V

V

G

G

I

I

R

Y

T

D

I

F

E

E

D

T

I

A

R

S

K

E

L

L

L

I

N

L

S

R

G

G

A

A

D

D

K

K

V

V

S
x
E

I

I

N

N

T

T

A

A

V

V

K

E

N

N

P

P

D

S

F

L

V

I

K

T

D

Q

A

I

A

A

L

Q

R

T

F

F

G

G

S

S

Q

Q

C

A

I

V

V

V

A

Y

I

I

D

A

A

A

K

K

R

R

K

-

T

V

N

D

G

G

S

E

G

-

W

F

E

M

V

V

Y

F

T

T

H

Y

G

S

G

G

R

T

N

K

P

N

T

T

G

G

I

I

D

L

V

L

L

R

E

D

W

W

A

V

V

V

K

E

M

V

E

E

S

K

F

R

G

G

A

A

G

G

E

E

I

I

L

V

L

L

T

G

S

S

M

I

D

D

K

R

D

L

G

G

T

T

K

K

D

S

G

G

Y

Y

D

D

L

T

E

E

L

M

T

I

A

R

A

F

V

V

S

R

D

P

A

L

I

T

R

T

I

L

P

P

V

I

I

I

A

A

S

H

G

R

G

G

V

A

G

G

T

K

K

T

E

E

D

H

I

F

L

L

D

E

G

A

F

F

T

L

K

A

G

G

K

-

A

A

D

D

A

A

L
x
K

A

A

A

D

S

S

I

V

F

F

H

H

F

S

G

R

I

E

Y

I

K

D

I

V

F

R

E

E

I

L

K

K

Q

E

F

Y

L

L

K

K

E

K

N

H

G

G

V

V

Q

N

V

V

R

R

L

L

binding magnesium ion: E100 (≠ S101), K221 (≠ L225)

1ox4B Towards understanding the mechanism of the complex cyclization reaction catalyzed by imidazole glycerophosphate synthase (see paper)
38% identity, 100% coverage: 2:253/253 of query aligns to 239:537/538 of 1ox4B

query
sites
1ox4B

L

L

A

T

K

R

R

R

I

I

P

A

C

C

L

L

D
|
D

V

V

K

R

D

T

G

N

R

D

V

-

K

Q

G

G

T

D

Q

L

F

V

L

V

N

T

L

K

I

G

D

D

A

L

G

G

D

K

P

P

V

V

Q

Q

V

L

A

A

E

Q

E

K

Y

Y

D

Y

R

Q

Q

Q

G

G

A

A

D

D

E

E

L

V

T

T

F

F

L

L

D

N

I

I

T

T

A

S

S

F

H

R

E

D

-

C

-

P

-

L

N

K

R

D

G

T

I

P

I

M

L

L

D

E

V

V

V

L

A

K

K

Q

T

A

A

A

E

K

K

T

V

V

F

F

M

V

P

P

L

L

T

T

V

V

G

G

I

I

R

K

T

D

I

I

E

V

D

D

I

V

-

D

-

G

-

T

-

K

-

I

-

P

-

A

-

L

-

E

-

V

-

A

R

S

K

L

L

Y

L

F

N

R

S

S

G

G

A

A

D

D

K

K

V

V

S

S

I

I

N

G

T

T

A

D

A

A

V

V

K

Y

N

A

P

A

D

E

F

K

V

Y

K

Y

D

E

A

L

A

G

L

N

R

R

-

G

-

D

-

G

-

T

-

S

-

P

-

I

-

E

-

T

-

I

-

S

-

K

-

A

F

Y

G

G

S

A

Q

Q

C

A

I

V

V

V

V

I

A

S

I

V

D
|
D

A

P

K

K

R

R

-

V

-

Y

-

V

-

N

-

S

-

Q

-

A

-

D

-

T

-

K

-

N

-

K

-

V

-

F

-

E

-

T

-

E

-

Y

-

P

K

G

T

P

N

N

G

G

S

E

G

K

W

Y

E

C

V

W

Y

Y

T

Q

-

C

-

T

-

I

H

K

G

G

R

R

N

E

P

S

T

R

G

D

I

L

D

G

V

V

L

W

E

E

W

L

A

T

V

R

K

A

M

C

E

E

S

A

F

L

G

G

A

A

G

G

E

E

I

I

L

L

T

N

S

C

M

I

D

D

K

K

D

D

G
|
G

T

S

K

N

D

S

G

G

Y

Y

D

D

L

L

E

E

L

L

T

I

A

E

A

H

V

V

S

K

D

D

A

A

I

V

R

K

I

I

P

P

V

V

I

I

A

A

S

S

G
x
S

G

G

V

A

G

G

T

V

K

P

E

E

D

H

I

F

L

E

D

E

G

A

F

F

T

L

K

K

G

T

K

R

A

A

D

D

A

A

A

C

L

L

A

G

A
|
A

S
x
G

I

M

F

F

H

H

F

R

G

G

I

E

Y

F

K

T

I

V

F

N

E

D

I

V

K

K

Q

E

F

Y

L

L

K

L

E

E

N

H

G

G

V

L

Q

K

V

V

R

R

L

M

active site: D248 (= D11), D392 (= D130)
binding pyrophosphate 2-: G463 (= G179), S488 (≠ G204), A511 (= A227), G512 (≠ S228)

P33734 Imidazole glycerol phosphate synthase hisHF; IGP synthase; IGPS; ImGP synthase; EC 4.3.2.10; EC 3.5.1.2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
37% identity, 100% coverage: 2:253/253 of query aligns to 236:549/552 of P33734

query
sites
P33734

L

L

A

T

K

R

R
|
R

I

I

P

A

C

C

L

L

D

D

V

V

K

R

-

T

-

N

-

D

-

Q

-

G

D

D

G

L

R

V

V

V

V

T

K
|
K

G

G

T

D

Q

Q

F

Y

-

D

-

V

-

R

-

E

-

K

-

S

-

D

-

G

L

K

N

G

L

V

I

R

D

N

A

L

G

G

D

K

P

P

V

V

Q

Q

V

L

A

A

E

Q

E

K

Y

Y

D

Y

R

Q

Q

Q

G

G

A

A

D

D

E

E

L

V

T

T

F

F

L

L

D

N

I

I

T

T

A

S

S

F

H

R

E

D

-

C

-

P

-

L

N

K

R

D

G

T

I

P

I

M

L

L

D

E

V

V

V

L

A

K

K

Q

T

A

A

A

E

K

K

T

V

V

F

F

M

V

P

P

L

L

T

T

V

V

G

G

I

I

R

K

T

D

I

I

E

V

D

D

I

V

-

D

-

G

-

T

-

K

-

I

-

P

-

A

-

L

-

E

-

V

-

A

R

S

K

L

L

Y

L

F

N

R

S

S

G

G

A

A

D

D

K
|
K

V

V

S

S

I

I

N

G

T

T

A

D

A

A

V

V

K

Y

N

A

P

A

D

E

F

K

V

Y

K

Y

D

E

A

L

A

G

L

N

R

R

-

G

-

D

-

G

-

T

-

S

-

P

-

I

-

E

-

T

-

I

-

S

-

K

-

A

F

Y

G

G

S

A

Q

Q

C

A

I

V

V

V

V

I

A

S

I

V

D

D

A

P

K

K

R

R

-

V

-

Y

-

V

-

N

-

S

-

Q

-

A

-

D

-

T

-

K

-

N

-

K

-

V

-

F

-

E

-

T

-

E

-

Y

-

P

K

G

T

P

N

N

G

G

S

E

G

K

W

Y

E

C

V

W

Y

Y

T

Q

-

C

-

T

-

I

H

K

G

G

R

R

N

E

P

S

T

R

G

D

I

L

D

G

V

V

L

W

E

E

W

L

A

T

V

R

K

A

M

C

E

E

S

A

F

L

G

G

A

A

G

G

E

E

I

I

L

L

T

N

S

C

M

I

D

D

K

K

D

D

G

G

T

S

K

N

D

S

G

G

Y

Y

D

D

L

L

E

E

L

L

T

I

A

E

A

H

V

V

S

K

D

D

A

A

I

V

R

K

I

I

P

P

V

V

I

I

A

A

S

S

G

S

G

G

V

A

G

G

T

V

K

P

E

E

D

H

I

F

L

E

D

E

G

A

F

F

T

L

K

K

G

T

K

R

A

A

D

D

A

A

A

C

L

L

A

G

A

A

S

G

I

M

F

F

H

H

F

R

G

G

I

E

Y

F

K

T

I

V

F

N

E

D

I

V

K

K

Q

E

F

Y

L

L

K

L

E

E

N

H

G

G

V

L

Q

K

V

V

R

R

L

M

R239 (= R5) mutation R->A,H,K: 1000-fold decrease in catalytic efficiency. Uncoupling of glutaminase activity from the cyclase activity.
K258 (= K19) mutation to A: No activity. Uncoupling of glutaminase activity from the cyclase activity.; mutation to R: Small reduction of activity.
K360 (= K99) mutation K->A,R: Almost no effect on activity.

1ox5A Towards understanding the mechanism of the complex cyclization reaction catalyzed by imidazole glycerophosphate synthase (see paper)
38% identity, 100% coverage: 2:253/253 of query aligns to 239:531/532 of 1ox5A

query
sites
1ox5A

L

L

A

T

K

R

R

R

I

I

P

A

C

C

L

L

D
|
D

V

V

K

R

D

T

G

N

R

D

V

-

K

Q

G

G

T

D

Q

-

F

-

L

L

N

V

L

V

I

T

D

K

A

L

G

G

D

K

P

P

V

V

Q

Q

V

L

A

A

E

Q

E

K

Y

Y

D

Y

R

Q

Q

Q

G

G

A

A

D

D

E

E

L

V

T

T

F

F

L
|
L

D

N

I
|
I

T

T

A

C

S

P

H

L

E

K

N

D

R

T

G

P

I

M

I

-

L

L

D

E

V

V

V

L

A

K

K

Q

T

A

A

A

E

K

K

T

V

V

F

F

M

V

P

P

L

L

T

T

V

V

G

G

G
|
G

G

G

I

I

R

K

T

D

I

I

E

V

D

D

I

V

-

D

-

G

-

T

-

K

-

I

-

P

-

A

-

L

-

E

-

V

-

A

R

S

K

L

L

Y

L

F

N

R

S

S

G

G

A

A

D

D

K

K

V

V

S

S

I

I

N
x
G

T
|
T

A

D

A

A

V

V

K

Y

N

A

P

A

D

E

F

K

V

Y

K

Y

D

E

A

L

A

G

L

N

R

R

-

G

-

D

-

G

-

T

-

S

-

P

-

I

-

E

-

T

-

I

-

S

-

K

-

A

F

Y

G

G

S

A

Q

Q

C

A

I

V

V

V

V

I

A
x
S

I

V

D
|
D

A

P

K

K

R

R

-

V

-

Y

-

V

-

N

-

S

-

Q

-

A

-

D

-

T

-

K

-

N

-

K

-

V

-

F

-

E

-

T

-

E

-

Y

-

P

K

G

T

P

N

N

G

G

S

E

G

K

W

Y

E

C

V

W

Y

Y

T

Q

-

C

-

T

-

I

H

K

G
|
G

G

G

R

R

N

E

P

S

T

R

G

D

I

L

D

G

V

V

L

W

E

E

W

L

A

T

V

R

K

A

M

C

E

E

S

A

F

L

G

G

A

A

G

G

E

E

I

I

L

L

T
x
N

S

C

M

I

D

D

K

K

D
|
D

G
|
G

T

S

K

N

D

S

G

G

Y

Y

D

D

L

L

E

E

L

L

T

I

A

E

A

H

V

V

S

K

D

D

A

A

I

V

R

K

I

I

P

P

V

V

I

I

A

A

S

S

G
x
S

G

G

V

A

G

G

T

V

K

P

E

E

D

H

I

F

L

E

D

E

G

A

F

F

T

L

K

K

G

T

K

R

A

A

D

D

A

A

A

C

L

L

A

G

A
|
A

S
x
G

I

M

F

F

H

H

F

R

G

G

I

E

Y

F

K

T

I

V

F

N

E

D

I

V

K

K

Q

E

F

Y

L

L

K

L

E

E

N

H

G

G

V

L

Q

K

V

V

R

R

L

M

active site: D248 (= D11), D386 (= D130)
binding phosphoric acid mono-[5-({[5-carbamoyl-3-(5-phosphonooxy-5-deoxy-ribofuranosyl)- 3h-imidazol-4-ylamino]-methyl}-amino)-2,3,4-trihydroxy-pentyl] ester: L283 (= L50), I285 (= I52), G313 (= G81), G346 (≠ N103), T347 (= T104), S384 (≠ A128), D386 (= D130), G424 (= G146), N451 (≠ T173), D456 (= D178), G457 (= G179), S482 (≠ G204), A505 (= A227), G506 (≠ S228)

5abtA S.Enterica hisa mutant d7n, g102a, v106m, d176a
33% identity, 82% coverage: 6:212/253 of query aligns to 2:211/246 of 5abtA

query
sites
5abtA

I

I

P

P

C

A

L

L

D
x
N

V

L

K

I

D

D

G

G

R

T

V

V

K

R

G

L

T

H

Q

Q

-
x
G

-

D

F

Y

L

A

N

R

L

Q

I

R

D

D

A

Y

G

G

-

N

D

D

P

P

V

L

Q

P

V

R

A

L

E

Q

E

D

Y

Y

D

A

R

A

Q

Q

G

G

A

A

D

G

E

V

L

L

T

H

F

L

L
x
V

D

D

I
x
L

T

T

A

G

S

A

H

K

E

D

N

P

R

A

G

K

I

R

I

Q

L

I

D

P

V

L

V

I

A

K

K

T

T

L

A

V

E

A

K

G

V

V

F

N

M

V

P

P

L

V

T

Q

V

V

G

G

G
|
G

I

V

R
|
R

T

T

I

E

E

E

D

D

I

V

R

A

K

A

L

L

N

K

S

A

G

G

A

V

D

A

K

R

V

V

S
x
V

I

I

N
x
A

T
x
S

A

T

A

A

V

M

K

K

N

S

P

P

D

D

F

V

V

V

K

K

D

G

A

W

A

F

L

E

R

R

F

F

G

G

S

A

Q

Q

C

A

I

L

V

V

V

L

A

A

I

L

D
|
D

A

V

K

R

R

I

K

D

T

E

N

H

G

G

S

T

G

K

W

-

E

Q

V

V

Y

A

T

V

H

S

G
|
G

G
x
W

R

Q

N

E

P

N

T

S

G

G

I

V

D

S

V

L

L

E

E

Q

W

L

A

V

V

E

K

T

M

Y

E

L

S

P

F

V

G

G

A

L

G

K

E

H

I

V

L

L

L

C

T

T

S

D

M

I

D

S

K

R

D

A

G
|
G

T

T

K

L

D

A

G

G

Y

S

D

N

L

V

E

S

L

L

T

Y

A

E

V

V

S

-

D

-

A

C

I

A

R

R

I

Y

P

P

V

Q

I

I

A

A

-

F

-

Q

-

S

S
|
S

G
|
G

G

G

V

I

G

G

T

D

K

I

E

D

D

D

I

I

active site: N7 (≠ D11), D129 (= D130)
binding [(2R,3S,4R,5R)-5-[4-aminocarbonyl-5-[(E)-[[(2R,3R,4S,5R)-3,4-bis(oxidanyl)-5-(phosphonooxymethyl)oxolan-2-yl]amino]methylideneamino]imidazol-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl dihydrogen phosphate: N7 (≠ D11), G19 (vs. gap), V49 (≠ L50), L51 (≠ I52), G81 (= G82), R83 (= R84), V100 (≠ S101), A102 (≠ N103), S103 (≠ T104), D129 (= D130), G144 (= G146), W145 (≠ G147), G177 (= G179), S202 (= S203), G203 (= G204)

Sites not aligning to the query:

binding [(2R,3S,4R,5R)-5-[4-aminocarbonyl-5-[(E)-[[(2R,3R,4S,5R)-3,4-bis(oxidanyl)-5-(phosphonooxymethyl)oxolan-2-yl]amino]methylideneamino]imidazol-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl dihydrogen phosphate: 223, 225, 226

3tdmA Computationally designed tim-barrel protein, halfflr (see paper)
51% identity, 38% coverage: 123:219/253 of query aligns to 1:95/120 of 3tdmA

query
sites
3tdmA

Q

Q

C

A

I

V

V

V

A

A

I

I

D

D

A

A

K

K

R

R

K

-

T

V

N

D

G

G

S

E

G

-

W

F

E

M

V

V

Y

F

T

T

H

Y

G

S

G

G

R

K

N

K

P

N

T

T

G

G

I

I

D

L

V

L

L

R

E

D

W

W

A

V

V

V

K

E

M

V

E

E

S

K

F

R

G

G

A

A

G

G

E

E

I

I

L

L

T

T

S

S

M

I

D

D

K

R

D
|
D

G
|
G

T

T

K

K

D

S

G

G

Y

Y

D

D

L

T

E

E

L

M

T

I

A

R

A

F

V

V

S

R

D

P

A

L

I

T

R

T

I

L

P

P

V

I

I

I

A

A

S

S

G

G

V

A

G

G

T

K

K

M

E

E

D

H

I

F

L

L

D

E

G

A

F

F

T

L

K

R

G

G

binding phosphate ion: D54 (= D178), G55 (= G179)

Sites not aligning to the query:

binding phosphate ion: 102, 103

2y85A Crystal structure of mycobacterium tuberculosis phosphoribosyl isomerase with bound rcdrp (see paper)
33% identity, 82% coverage: 6:212/253 of query aligns to 5:199/234 of 2y85A

query
sites
2y85A

I

L

P

P

C

A

L

V

D
|
D

V

V

K

V

D

E

G

G

R

R

V

A

V

V

K

R

G

-

T

T

Q

E

F

Y

L

G

N

S

L

A

I

V

D

D

A

A

G

-

D

-

P

-

V

-

Q

-

V

-

A

A

E

L

E

G

Y

W

D

Q

R

R

Q

D

G

G

A

A

D

E

E

W

L

I

T
x
H

F

L

L
x
V

D

D

I
x
L

T

D

A

A

S
x
A

H

F

E

-

N

G

R

R

G

G

I

S

I

N

L

H

D

E

V

L

A

A

K

E

T

V

A

V

E

G

K

K

V

L

F

D

M

V

P

Q

L

V

T

E

V

L

G
x
S

G

G

I

I

R

R

T

D

I

D

E

E

D

S

I

L

R

A

K

A

L

A

L

L

N

A

S

T

G

G

A

C

D

A

K

R

V

V

S

N

I

V

N

G

T

T

A

A

V

L

K

E

N

N

P

P

D

Q

F

W

V

C

K

A

D

R

A

V

A

I

L

G

R

E

F

H

G

G

S

D

Q

Q

C

V

I

A

V

V

-

G

-

L

-
x
D

-

V

V

Q

A

I

I

I

D

D

A

G

K

E

R

H

K

R

T

L

N

R

G

G

S
x
R

G

G

W

W

E

E

V

-

Y

-

T

T

H

D

G

G

R

D

N

L

P

-

T

-

G

-

I

W

D

D

V

V

L

L

E

E

W

-

A

-

V

-

K

R

M

L

E

D

S

S

F

E

G

G

A

C

G

S

E

R

I

F

L

V

T

T

S

D

M

I

D

T

K

K

D
|
D

G
|
G

T

T

K

L

D

G

G

G

Y

P

D

N

L

L

E

D

L

L

T

L

A

A

A

G

V

V

S

A

D

D

A

R

I

T

R

D

I

A

P

P

V

V

I

I

A

A

S
|
S

G
|
G

V

V

G

S

T

S

K

L

E

D

D

D

I

L

active site: D10 (= D11), D120 (vs. gap)
binding 1-(o-carboxy-phenylamino)-1-deoxy-d-ribulose-5-phosphate: D10 (= D11), H40 (≠ T48), V42 (≠ L50), L44 (≠ I52), A47 (≠ S55), S71 (≠ G80), R133 (≠ S138), D165 (= D178), G166 (= G179), S190 (= S203), G191 (= G204), G192 (= G205)

Sites not aligning to the query:

binding 1-(o-carboxy-phenylamino)-1-deoxy-d-ribulose-5-phosphate: 216, 217

3zs4A Crystal structure of mycobacterium tuberculosis phosphoribosyl isomerase with bound prfar
33% identity, 82% coverage: 6:212/253 of query aligns to 5:208/244 of 3zs4A

query
sites
3zs4A

I

L

P

P

C
x
A

L

V

D
|
D

V

V

K

V

D

E

G

G

R

R

V

A

V

V

K
x
R

G

L

T

V

Q

Q

F

G

L

K

-

A

-

G

N

S

L

Q

I

T

D

E

A

Y

G

G

D

S

P

A

V

V

Q

D

V

A

A

A

E

L

E

G

Y

W

D

Q

R

R

Q

D

G

G

A

A

D

E

E

W

L

I

T

H

F

L

L

V

D

D

I

L

T

D

A

A

S
x
A

H
x
F

E

-

N

G

R

R

G

G

I

S

I

N

L

H

D

E

V

L

A

A

K

E

T

V

A

V

E

G

K

K

V

L

F

D

M

V

P

Q

L

V

T

E

V

L

G
x
S

G

G

G
|
G

I

I

R
|
R

T

D

I

D

E

E

D

S

I

L

R

A

K

A

L

A

L

L

N

A

S

T

G

G

A

C

D

A

K

R

V

V

S

N

I

V

N
x
G

T
|
T

A

A

V

L

K

E

N

N

P

P

D

Q

F

W

V

C

K

A

D

R

A

V

A

I

L

G

R

E

F

H

G

G

S

D

Q

Q

C

V

I

A

V

V

-

G

-

L

-
x
D

-

V

V

Q

A

I

I

I

D

D

A

G

K

E

R

H

K

R

T

L

N

R

G

G

S

R

G
|
G

W
|
W

E

E

V

-

Y

-

T

T

H

D

G

G

R

D

N

L

P

-

T

-

G

-

I

W

D

D

V

V

L

L

E

E

W

-

A

-

V

-

K

R

M

L

E

D

S

S

F

E

G

G

A

C

G

S

E

R

I

F

L

V

T

T

S

D

M

I

D

T

K

K

D
|
D

G
|
G

T

T

K

L

D

G

G

G

Y

P

D

N

L

L

E

D

L

L

T

L

A

A

A

G

V

V

S

A

D

D

A

R

I

T

R

D

I

A

P

P

V

V

I

I

A

A

S

S

G
|
G

V

V

G

S

T

S

K

L

E

D

D

D

I

L

active site: D10 (= D11), D129 (vs. gap)
binding phosphoric acid mono-[5-({[5-carbamoyl-3-(5-phosphonooxy-5-deoxy-ribofuranosyl)- 3h-imidazol-4-ylamino]-methyl}-amino)-2,3,4-trihydroxy-pentyl] ester: A8 (≠ C9), D10 (= D11), R18 (≠ K19), A56 (≠ S55), F57 (≠ H56), S80 (≠ G80), G82 (= G82), R84 (= R84), G103 (≠ N103), T104 (= T104), D129 (vs. gap), G143 (= G139), W144 (= W140), D174 (= D178), G175 (= G179), G200 (= G204), G201 (= G205)

Sites not aligning to the query:

binding phosphoric acid mono-[5-({[5-carbamoyl-3-(5-phosphonooxy-5-deoxy-ribofuranosyl)- 3h-imidazol-4-ylamino]-methyl}-amino)-2,3,4-trihydroxy-pentyl] ester: 223, 225, 226

5ab3A S.Enterica hisa mutant d7n, d10g, dup13-15, q24l, g102a (see paper)
32% identity, 82% coverage: 6:212/253 of query aligns to 2:208/241 of 5ab3A

query
sites
5ab3A

I

I

P

P

C

A

L

L

D
x
N

V

L

K

I

D

G

G

G

R

T

V

V

K

R

G

V

T

V

Q
x
R

-

L

-

H

-

Q

-

G

-

D

F
x
Y

L

A

N

R

L

L

I

R

D

D

A

Y

G

G

-

N

D

D

P

P

V

L

Q

P

V

R

A

L

E

Q

E

D

Y

Y

D

A

R

A

Q

Q

G

G

A

A

D

G

E

V

L

L

T

H

F

L

L
x
V

D

D

I

L

T

T

A

G

S

A

H

K

E

D

N

P

R

A

G

K

I

R

I

Q

L

I

D

P

V

L

V

I

A

K

K

T

T

L

A

V

E

A

K

G

V

V

F

N

M

V

P

P

L

V

T

Q

V

V

G

G

G
|
G

I

V

R

R

T

T

I

E

E

E

D

D

I

V

R

A

K

A

L

L

N

K

S

A

G

G

A

V

D

A

K

R

V

V

S

V

I

I

N
x
A

T
x
S

A

T

A

A

V

V

K

K

N

S

P

P

D

D

F

V

V

V

K

K

D

G

A

W

A

F

L

E

R

R

F

F

G

G

S

A

Q

Q

C

A

I

L

V

V

V

L

A
|
A

I

L

D
|
D

A

V

K

R

R

I

K

D

T

E

N

H

G

G

S

T

G

K

W

Q

E

V

V

A

Y

V

T

-

H

-

G

-

R

-

N

-

P

-

T

S

G

G

I

V

D

S

V

L

L

E

E

Q

W

L

A

V

V

E

K

T

M

Y

E

L

S

P

F

V

G

G

A

L

G

K

E

H

I

V

L

L

L

C

T

T

S

D

M

I

D

S

K

R

D

D

G
|
G

T

T

K

L

D

A

G

G

Y

S

D

N

L

V

E

S

L

L

T

Y

A

E

V

V

S

-

D

-

A

C

I

A

R

R

I

Y

P

P

V

Q

I

I

A

A

-

F

-

Q

-

S

S
|
S

G
|
G

V

I

G

G

T

D

K

I

E

D

D

D

I

I

active site: N7 (≠ D11), D132 (= D130)
binding [(2r,3s,4r,5r)-5-[4-aminocarbonyl-5-[[(z)-[(3r,4r)-3,4-dihydroxy-2-oxo-5-phosphonooxy-pentyl]iminomethyl]amino]imidazol-1-yl]-3,4-dihydroxy-oxolan-2-yl]methyl dihydrogen phosphate: N7 (≠ D11), R18 (≠ Q22), Y24 (≠ F23), V52 (≠ L50), G84 (= G82), A105 (≠ N103), S106 (≠ T104), A130 (= A128), D132 (= D130), G174 (= G179), S199 (= S203), G200 (= G204), G201 (= G205)

Sites not aligning to the query:

binding [(2r,3s,4r,5r)-5-[4-aminocarbonyl-5-[[(z)-[(3r,4r)-3,4-dihydroxy-2-oxo-5-phosphonooxy-pentyl]iminomethyl]amino]imidazol-1-yl]-3,4-dihydroxy-oxolan-2-yl]methyl dihydrogen phosphate: 222, 223

Query Sequence

>WP_013450396.1 NCBI__GCF_000183405.1:WP_013450396.1
MLAKRIIPCLDVKDGRVVKGTQFLNLIDAGDPVQVAEEYDRQGADELTFLDITASHENRG
IILDVVAKTAEKVFMPLTVGGGIRTIEDIRKLLNSGADKVSINTAAVKNPDFVKDAALRF
GSQCIVVAIDAKRKTNGSGWEVYTHGGRNPTGIDVLEWAVKMESFGAGEILLTSMDKDGT
KDGYDLELTAAVSDAIRIPVIASGGVGTKEDILDGFTKGKADAALAASIFHFGIYKIFEI
KQFLKENGVQVRL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory