SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013450717.1 NCBI__GCF_000183405.1:WP_013450717.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1kl2A Crystal structure of serine hydroxymethyltransferase complexed with glycine and 5-formyl tetrahydrofolate (see paper)
66% identity, 95% coverage: 9:407/418 of query aligns to 6:404/405 of 1kl2A

query
sites
1kl2A

Q

Q

A

Q

D

D

P

P

E

Q

I

V

Y

F

D

A

A

A

L

I

K

E

K

Q

E

E

I

R

E

K

R

R

Q

Q

E

H

T

A

H

K

I

I

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

F

F

V

V

S

S

P

R

A

A

V

V

L

M

E

E

A

A

Q

Q

G

G

S

S

I

V

M

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

A

G

K

R

R

R

Y
|
Y

Y
|
Y

G

G

G

G

C

C

E

E

F

Y

V

V

D

D

I

I

A

V

E

E

E

E

L

L

A

A

I

R

K

E

R

R

A

A

K

K

E

Q

L

L

F

F

G

G

A

A

E

E

H

H

A

A

N

N

V

V

Q

Q

A

P

H

H

S
|
S

G
|
G

S
x
A

Q

Q

A

A

N

N

M

M

A

A

V

V

Y

Y

F

F

A

T

V

V

L

L

K

E

P

H

G

G

D

D

T

T

I

V

M

L

G

G

M

M

N

N

L

L

S

S

H

H

G

G

G
|
G

H
|
H

L

L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

F

F

S

S

G

G

K

V

L

Q

F

Y

N

N

V

F

I

V

S

A

Y

Y

G

G

V

V

N

D

K

P

E

E

T

T

E

H

T

V

I

I

D

D

Y

Y

D

D

E

D

A

V

E

R

K

E

L

K

A

A

T

R

E

L

H

H

K

R

P

P

K

K

L

L

I

I

M

V

V

A

G

A

A

A

S
|
S

A

A

Y

Y

P

P

R

R

T

I

I

I

D

D

F

F

K

A

K

K

F

F

R

R

E

E

I

I

A

A

D

D

K

E

V

V

G

G

A

A

V

Y

L

L

V

M

V

V

D
|
D

M

M

A
|
A

H
|
H

I

I

A

A

G

G

L

L

V

V

A

A

A

A

G

G

A

L

H

H

P

P

S

N

P

P

V

V

P

P

Y

Y

A

A

D

H

F

F

V

V

T

T

T

T

T
|
T

T

T

H
|
H

K
|
K

T

T

L

L

R

R

G

G

P

P

R
|
R

G

G

G

G

L

M

I

I

L

L

C

C

K

Q

E

E

E

Q

Y

F

A

A

K

K

T

Q

L

I

N

D

S

K

Q

A

I

I

F
|
F

P

P

G

G

I

I

Q

Q

G

G

G

G

P

P

L

L

M

M

H

H

V

V

I

I

A

A

A

A

K

K

A

A

V

V

A

A

L

F

K

G

E

E

A

A

M

L

S

Q

E

D

D

D

F

F

K

K

V

A

Y

Y

Q

A

H

K

Q

R

I

V

V

V

K

D

N

N

A

A

K

K

R

R

L

L

S

A

E

S

R

A

L

L

M

Q

K

N

H

E

G

G

F

F

K

T

L

L

V

V

S

S

G

G

G

G

T

T

D

D

N

N

H

H

L

L

M

L

L

L

I

V

N

D

L

L

S

R

N

P

S

Q

E

Q

I

L

T

T

G

G

K

K

E

T

A

A

E

E

E

K

A

V

L

L

G

D

R

E

A

V

N

G

I

I

T

T

V

V

N

N

K

K

N
|
N

T

T

V

I

P

P

F

Y

E

D

T

P

R

E

S

S

P

P

F

F

V

V

T

T

S

S

G

G

V

I

R
|
R

I

I

G

G

T

T

P

A

A

A

V

V

T

T

T

T

R

R

G

G

M

F

K

G

E

L

S

E

E

E

M

M

D

D

F

E

I

I

G

A

D

A

L

I

I

I

A

G

Q

L

V

V

L

L

S

K

D

N

I

V

K

G

S

S

D

E

L

Q

N

A

I

L

N

E

D

E

V

A

K

R

S

Q

K

R

V

V

L

A

E

A

L

L

C

T

active site: Y51 (= Y54), E53 (= E56), D197 (= D200), T223 (= T226), K226 (= K229), R232 (= R235)
binding N-{[4-({[(6R)-2-amino-5-formyl-4-oxo-1,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)phenyl]carbonyl}-L-glutamic acid: E53 (= E56), Y60 (= Y63), G121 (= G124), H122 (= H125), S172 (= S175), F251 (= F254), N341 (= N344)
binding glycine: S31 (= S34), Y51 (= Y54), Y61 (= Y64), H200 (= H203), R357 (= R360)
binding pyridoxal-5'-phosphate: S93 (= S96), G94 (= G97), A95 (≠ S98), H122 (= H125), S172 (= S175), D197 (= D200), A199 (= A202), H200 (= H203), T223 (= T226), H225 (= H228), K226 (= K229)

1kl1A Crystal structure of serine hydroxymethyltransferase complexed with glycine (see paper)
66% identity, 95% coverage: 9:407/418 of query aligns to 6:404/405 of 1kl1A

query
sites
1kl1A

Q

Q

A

Q

D

D

P

P

E

Q

I

V

Y

F

D

A

A

A

L

I

K

E

K

Q

E

E

I

R

E

K

R

R

Q

Q

E

H

T

A

H

K

I

I

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

F

F

V

V

S

S

P

R

A

A

V

V

L

M

E

E

A

A

Q

Q

G

G

S

S

I

V

M

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

A

G

K

R

R

R

Y

Y

Y

Y

G

G

G

G

C

C

E

E

F

Y

V

V

D

D

I

I

A

V

E

E

E

E

L

L

A

A

I

R

K

E

R

R

A

A

K

K

E

Q

L

L

F

F

G

G

A

A

E

E

H

H

A

A

N

N

V

V

Q

Q

A

P

H

H

S
|
S

G
|
G

S
x
A

Q

Q

A

A

N

N

M

M

A

A

V

V

Y

Y

F

F

A

T

V

V

L

L

K

E

P

H

G

G

D

D

T

T

I

V

M

L

G

G

M

M

N

N

L

L

S

S

H

H

G

G

G

G

H
|
H

L

L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

F

F

S

S

G

G

K

V

L

Q

F

Y

N

N

V

F

I

V

S

A

Y

Y

G

G

V

V

N

D

K

P

E

E

T

T

E

H

T

V

I

I

D

D

Y

Y

D

D

E

D

A

V

E

R

K

E

L

K

A

A

T

R

E

L

H

H

K

R

P

P

K

K

L

L

I

I

M

V

V

A

G

A

A
|
A

S
|
S

A

A

Y

Y

P

P

R

R

T

I

I

I

D

D

F

F

K

A

K

K

F

F

R

R

E

E

I

I

A

A

D

D

K

E

V

V

G

G

A

A

V

Y

L

L

V

M

V

V

D
|
D

M

M

A
|
A

H
|
H

I

I

A

A

G

G

L

L

V

V

A

A

A

A

G

G

A

L

H

H

P

P

S

N

P

P

V

V

P

P

Y

Y

A

A

D

H

F

F

V

V

T

T

T

T

T
|
T

T

T

H
|
H

K
|
K

T

T

L

L

R

R

G

G

P

P

R
|
R

G

G

G

G

L

M

I

I

L

L

C

C

K

Q

E

E

E

Q

Y

F

A

A

K

K

T

Q

L

I

N

D

S

K

Q

A

I

I

F

F

P

P

G

G

I

I

Q

Q

G

G

G

G

P

P

L

L

M

M

H

H

V

V

I

I

A

A

A

A

K

K

A

A

V

V

A

A

L

F

K

G

E

E

A

A

M

L

S

Q

E

D

D

D

F

F

K

K

V

A

Y

Y

Q

A

H

K

Q

R

I

V

V

V

K

D

N

N

A

A

K

K

R

R

L

L

S

A

E

S

R

A

L

L

M

Q

K

N

H

E

G

G

F

F

K

T

L

L

V

V

S

S

G

G

G

G

T

T

D

D

N

N

H

H

L

L

M

L

L

L

I

V

N

D

L

L

S

R

N

P

S

Q

E

Q

I

L

T

T

G

G

K

K

E

T

A

A

E

E

E

K

A

V

L

L

G

D

R

E

A

V

N

G

I

I

T

T

V

V

N

N

K

K

N

N

T

T

V

I

P

P

F

Y

E

D

T

P

R

E

S

S

P

P

F

F

V

V

T

T

S

S

G

G

V

I

R
|
R

I

I

G

G

T

T

P

A

A

A

V

V

T

T

T

T

R

R

G

G

M

F

K

G

E

L

S

E

E

E

M

M

D

D

F

E

I

I

G

A

D

A

L

I

I

I

A

G

Q

L

V

V

L

L

S

K

D

N

I

V

K

G

S

S

D

E

L

Q

N

A

I

L

N

E

D

E

V

A

K

R

S

Q

K

R

V

V

L

A

E

A

L

L

C

T

active site: Y51 (= Y54), E53 (= E56), D197 (= D200), T223 (= T226), K226 (= K229), R232 (= R235)
binding glycine: S31 (= S34), H122 (= H125), R357 (= R360)
binding pyridoxal-5'-phosphate: S93 (= S96), G94 (= G97), A95 (≠ S98), H122 (= H125), A171 (= A174), S172 (= S175), D197 (= D200), A199 (= A202), H200 (= H203), T223 (= T226), H225 (= H228), K226 (= K229)

1kkpA Crystal structure of serine hydroxymethyltransferase complexed with serine (see paper)
66% identity, 95% coverage: 9:407/418 of query aligns to 6:404/405 of 1kkpA

query
sites
1kkpA

Q

Q

A

Q

D

D

P

P

E

Q

I

V

Y

F

D

A

A

A

L

I

K

E

K

Q

E

E

I

R

E

K

R

R

Q

Q

E

H

T

A

H

K

I

I

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

F

F

V

V

S

S

P

R

A

A

V

V

L

M

E

E

A

A

Q

Q

G

G

S

S

I

V

M

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

A

G

K

R

R

R

Y

Y

Y

Y

G

G

G

G

C

C

E

E

F

Y

V

V

D

D

I

I

A

V

E

E

E

E

L

L

A

A

I

R

K

E

R

R

A

A

K

K

E

Q

L

L

F

F

G

G

A

A

E

E

H

H

A

A

N

N

V

V

Q

Q

A

P

H

H

S
|
S

G
|
G

S
x
A

Q

Q

A

A

N

N

M

M

A

A

V

V

Y

Y

F

F

A

T

V

V

L

L

K

E

P

H

G

G

D

D

T

T

I

V

M

L

G

G

M

M

N

N

L

L

S

S

H

H

G

G

G

G

H
|
H

L

L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

F

F

S

S

G

G

K

V

L

Q

F

Y

N

N

V

F

I

V

S

A

Y

Y

G

G

V

V

N

D

K

P

E

E

T

T

E

H

T

V

I

I

D

D

Y

Y

D

D

E

D

A

V

E

R

K

E

L

K

A

A

T

R

E

L

H

H

K

R

P

P

K

K

L

L

I

I

M

V

V

A

G

A

A

A

S
|
S

A

A

Y

Y

P

P

R

R

T

I

I

I

D

D

F

F

K

A

K

K

F

F

R

R

E

E

I

I

A

A

D

D

K

E

V

V

G

G

A

A

V

Y

L

L

V

M

V

V

D
|
D

M

M

A
|
A

H
|
H

I

I

A

A

G

G

L

L

V

V

A

A

A

A

G

G

A

L

H

H

P

P

S

N

P

P

V

V

P

P

Y

Y

A

A

D

H

F

F

V

V

T

T

T

T

T
|
T

T

T

H

H

K
|
K

T

T

L

L

R

R

G

G

P

P

R
|
R

G

G

G

G

L

M

I

I

L

L

C

C

K

Q

E

E

E

Q

Y

F

A

A

K

K

T

Q

L

I

N

D

S

K

Q

A

I

I

F

F

P

P

G

G

I

I

Q

Q

G

G

G

G

P

P

L

L

M

M

H

H

V

V

I

I

A

A

A

A

K

K

A

A

V

V

A

A

L

F

K

G

E

E

A

A

M

L

S

Q

E

D

D

D

F

F

K

K

V

A

Y

Y

Q

A

H

K

Q

R

I

V

V

V

K

D

N

N

A

A

K

K

R

R

L

L

S

A

E

S

R

A

L

L

M

Q

K

N

H

E

G

G

F

F

K

T

L

L

V

V

S

S

G

G

G

G

T

T

D

D

N

N

H

H

L

L

M

L

L

L

I

V

N

D

L

L

S

R

N

P

S

Q

E

Q

I

L

T

T

G

G

K

K

E

T

A

A

E

E

E

K

A

V

L

L

G

D

R

E

A

V

N

G

I

I

T

T

V

V

N

N

K

K

N

N

T

T

V

I

P

P

F

Y

E

D

T

P

R

E

S

S

P

P

F

F

V

V

T

T

S

S

G

G

V

I

R
|
R

I

I

G

G

T

T

P

A

A

A

V

V

T

T

T

T

R

R

G

G

M

F

K

G

E

L

S

E

E

E

M

M

D

D

F

E

I

I

G

A

D

A

L

I

I

I

A

G

Q

L

V

V

L

L

S

K

D

N

I

V

K

G

S

S

D

E

L

Q

N

A

I

L

N

E

D

E

V

A

K

R

S

Q

K

R

V

V

L

A

E

A

L

L

C

T

active site: Y51 (= Y54), E53 (= E56), D197 (= D200), T223 (= T226), K226 (= K229), R232 (= R235)
binding pyridoxal-5'-phosphate: S93 (= S96), G94 (= G97), A95 (≠ S98), H122 (= H125), S172 (= S175), D197 (= D200), A199 (= A202), H200 (= H203), K226 (= K229)
binding serine: S31 (= S34), H122 (= H125), R357 (= R360)

1kkjA Crystal structure of serine hydroxymethyltransferase from b.Stearothermophilus (see paper)
66% identity, 95% coverage: 9:407/418 of query aligns to 6:404/405 of 1kkjA

query
sites
1kkjA

Q

Q

A

Q

D

D

P

P

E

Q

I

V

Y

F

D

A

A

A

L

I

K

E

K

Q

E

E

I

R

E

K

R

R

Q

Q

E

H

T

A

H

K

I

I

E

E

L

L

I

I

A

A

S

S

E

E

N

N

F

F

V

V

S

S

P

R

A

A

V

V

L

M

E

E

A

A

Q

Q

G

G

S

S

I

V

M

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

A

G

K

R

R

R

Y

Y

Y

Y

G

G

G

G

C

C

E

E

F

Y

V

V

D

D

I

I

A

V

E

E

E

E

L

L

A

A

I

R

K

E

R

R

A

A

K

K

E

Q

L

L

F

F

G

G

A

A

E

E

H

H

A

A

N

N

V

V

Q

Q

A

P

H

H

S
|
S

G
|
G

S
x
A

Q

Q

A

A

N

N

M

M

A

A

V

V

Y

Y

F

F

A

T

V

V

L

L

K

E

P

H

G

G

D

D

T

T

I

V

M

L

G

G

M

M

N

N

L

L

S

S

H

H

G

G

G

G

H
|
H

L

L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

F

F

S

S

G

G

K

V

L

Q

F

Y

N

N

V

F

I

V

S

A

Y

Y

G

G

V

V

N

D

K

P

E

E

T

T

E

H

T

V

I

I

D

D

Y

Y

D

D

E

D

A

V

E

R

K

E

L

K

A

A

T

R

E

L

H

H

K

R

P

P

K

K

L

L

I

I

M

V

V

A

G

A

A

A

S
|
S

A

A

Y

Y

P

P

R

R

T

I

I

I

D

D

F

F

K

A

K

K

F

F

R

R

E

E

I

I

A

A

D

D

K

E

V

V

G

G

A

A

V

Y

L

L

V

M

V

V

D
|
D

M

M

A
|
A

H
|
H

I

I

A

A

G

G

L

L

V

V

A

A

A

A

G

G

A

L

H

H

P

P

S

N

P

P

V

V

P

P

Y

Y

A

A

D

H

F

F

V

V

T

T

T

T

T
|
T

T

T

H
|
H

K
|
K

T

T

L

L

R

R

G

G

P

P

R
|
R

G

G

G

G

L

M

I

I

L

L

C

C

K

Q

E

E

E

Q

Y

F

A

A

K

K

T

Q

L

I

N

D

S

K

Q

A

I

I

F

F

P

P

G

G

I

I

Q

Q

G

G

G

G

P

P

L

L

M

M

H

H

V

V

I

I

A

A

A

A

K

K

A

A

V

V

A

A

L

F

K

G

E

E

A

A

M

L

S

Q

E

D

D

D

F

F

K

K

V

A

Y

Y

Q

A

H

K

Q

R

I

V

V

V

K

D

N

N

A

A

K

K

R

R

L

L

S

A

E

S

R

A

L

L

M

Q

K

N

H

E

G

G

F

F

K

T

L

L

V

V

S

S

G

G

G

G

T

T

D

D

N

N

H

H

L

L

M

L

L

L

I

V

N

D

L

L

S

R

N

P

S

Q

E

Q

I

L

T

T

G

G

K

K

E

T

A

A

E

E

E

K

A

V

L

L

G

D

R

E

A

V

N

G

I

I

T

T

V

V

N

N

K

K

N

N

T

T

V

I

P

P

F

Y

E

D

T

P

R

E

S

S

P

P

F

F

V

V

T

T

S

S

G

G

V

I

R

R

I

I

G

G

T

T

P

A

A

A

V

V

T

T

T

T

R

R

G

G

M

F

K

G

E

L

S

E

E

E

M

M

D

D

F

E

I

I

G

A

D

A

L

I

I

I

A

G

Q

L

V

V

L

L

S

K

D

N

I

V

K

G

S

S

D

E

L

Q

N

A

I

L

N

E

D

E

V

A

K

R

S

Q

K

R

V

V

L

A

E

A

L

L

C

T

active site: Y51 (= Y54), E53 (= E56), D197 (= D200), T223 (= T226), K226 (= K229), R232 (= R235)
binding pyridoxal-5'-phosphate: S93 (= S96), G94 (= G97), A95 (≠ S98), H122 (= H125), S172 (= S175), D197 (= D200), A199 (= A202), H200 (= H203), T223 (= T226), H225 (= H228), K226 (= K229)

2vmyA Crystal structure of f351gbsshmt in complex with gly and fthf (see paper)
66% identity, 95% coverage: 9:407/418 of query aligns to 6:404/405 of 2vmyA

query
sites
2vmyA

Q

Q

A

Q

D

D

P

P

E

Q

I

V

Y

F

D

A

A

A

L

I

K

E

K

Q

E

E

I

R

E

K

R

R

Q

Q

E

H

T

A

H

K

I

I

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

F

F

V

V

S

S

P

R

A

A

V

V

L

M

E

E

A

A

Q

Q

G

G

S

S

I

V

M

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

A

G

K

R

R

R

Y
|
Y

Y
|
Y

G

G

G

G

C

C

E

E

F

Y

V

V

D

D

I

I

A

V

E

E

E

E

L

L

A

A

I

R

K

E

R

R

A

A

K

K

E

Q

L

L

F

F

G

G

A

A

E

E

H

H

A

A

N

N

V

V

Q

Q

A

P

H

H

S
|
S

G
|
G

S
x
A

Q

Q

A

A

N

N

M

M

A

A

V

V

Y

Y

F

F

A

T

V

V

L

L

K

E

P

H

G

G

D

D

T

T

I

V

M

L

G

G

M

M

N

N

L
|
L

S

S

H

H

G

G

G
|
G

H
|
H

L
|
L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

F

F

S

S

G

G

K

V

L

Q

F

Y

N

N

V

F

I

V

S

A

Y

Y

G

G

V

V

N

D

K

P

E

E

T

T

E

H

T

V

I

I

D

D

Y

Y

D

D

E

D

A

V

E

R

K

E

L

K

A

A

T

R

E

L

H

H

K

R

P

P

K

K

L

L

I

I

M

V

V

A

G

A

A

A

S
|
S

A

A

Y

Y

P

P

R

R

T

I

I

I

D

D

F

F

K

A

K

K

F

F

R

R

E

E

I

I

A

A

D

D

K

E

V

V

G

G

A

A

V

Y

L

L

V

M

V

V

D
|
D

M

M

A
|
A

H
|
H

I

I

A

A

G

G

L

L

V

V

A

A

A

A

G

G

A

L

H

H

P

P

S

N

P

P

V

V

P

P

Y

Y

A

A

D

H

F

F

V

V

T

T

T

T

T
|
T

T

T

H

H

K
|
K

T

T

L

L

R

R

G

G

P

P

R
|
R

G

G

G

G

L

M

I

I

L

L

C

C

K

Q

E

E

E

Q

Y

F

A

A

K

K

T

Q

L

I

N

D

S
x
K

Q

A

I

I

F
|
F

P

P

G

G

I

I

Q

Q

G

G

G
|
G

P

P

L

L

M

M

H

H

V

V

I

I

A

A

A

A

K

K

A

A

V

V

A

A

L

F

K

G

E

E

A

A

M

L

S

Q

E

D

D

D

F

F

K

K

V

A

Y

Y

Q

A

H

K

Q

R

I

V

V

V

K

D

N

N

A

A

K

K

R

R

L

L

S

A

E

S

R

A

L

L

M

Q

K

N

H

E

G

G

F

F

K

T

L

L

V

V

S

S

G

G

G

G

T

T

D

D

N

N

H

H

L

L

M

L

L

L

I

V

N

D

L

L

S

R

N

P

S

Q

E

Q

I

L

T

T

G

G

K

K

E

T

A

A

E

E

E

K

A

V

L

L

G

D

R

E

A

V

N

G

I

I

T

T

V

V

N

N

K

K

N
|
N

T

T

V

I

P

P

F

Y

E

D

T

P

R

E

S
|
S

P
|
P

F
x
G

V

V

T

T

S

S

G

G

V

I

R
|
R

I

I

G

G

T

T

P

A

A

A

V

V

T

T

T

T

R

R

G

G

M

F

K

G

E

L

S

E

E

E

M

M

D

D

F

E

I

I

G

A

D

A

L

I

I

I

A

G

Q

L

V

V

L

L

S

K

D

N

I

V

K

G

S

S

D

E

L

Q

N

A

I

L

N

E

D

E

V

A

K

R

S

Q

K

R

V

V

L

A

E

A

L

L

C

T

active site: Y51 (= Y54), E53 (= E56), D197 (= D200), T223 (= T226), K226 (= K229), R232 (= R235)
binding N-[4-({[(6S)-2-amino-5-formyl-4-oxo-3,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)benzoyl]-L-glutamic acid: E53 (= E56), Y60 (= Y63), Y61 (= Y64), L117 (= L120), G121 (= G124), H122 (= H125), L123 (= L126), S172 (= S175), K248 (≠ S251), F251 (= F254), N341 (= N344), S349 (= S352), P350 (= P353), G351 (≠ F354), R357 (= R360)
binding glycine: S31 (= S34), Y51 (= Y54), Y61 (= Y64), H200 (= H203), K226 (= K229), R357 (= R360)
binding pyridoxal-5'-phosphate: Y51 (= Y54), S93 (= S96), G94 (= G97), A95 (≠ S98), H122 (= H125), S172 (= S175), D197 (= D200), A199 (= A202), H200 (= H203), T223 (= T226), K226 (= K229), G257 (= G260)

2vmxA Crystal structure of f351gbsshmt in complex with l-allo-thr (see paper)
66% identity, 95% coverage: 9:407/418 of query aligns to 6:404/405 of 2vmxA

query
sites
2vmxA

Q

Q

A

Q

D

D

P

P

E

Q

I

V

Y

F

D

A

A

A

L

I

K

E

K

Q

E

E

I

R

E

K

R

R

Q

Q

E

H

T

A

H

K

I

I

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

F

F

V

V

S

S

P

R

A

A

V

V

L

M

E

E

A

A

Q

Q

G

G

S

S

I

V

M

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

A

G

K

R

R

R

Y

Y

Y

Y

G

G

G

G

C

C

E

E

F

Y

V

V

D

D

I

I

A

V

E

E

E

E

L

L

A

A

I

R

K

E

R

R

A

A

K

K

E

Q

L

L

F

F

G

G

A

A

E

E

H

H

A

A

N

N

V

V

Q

Q

A

P

H

H

S
|
S

G
|
G

S
x
A

Q

Q

A

A

N

N

M

M

A

A

V

V

Y

Y

F

F

A

T

V

V

L

L

K

E

P

H

G

G

D

D

T

T

I

V

M

L

G

G

M

M

N

N

L

L

S

S

H

H

G

G

G

G

H
|
H

L

L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

F

F

S

S

G

G

K

V

L

Q

F

Y

N

N

V

F

I

V

S

A

Y

Y

G

G

V

V

N

D

K

P

E

E

T

T

E

H

T

V

I

I

D

D

Y

Y

D

D

E

D

A

V

E

R

K

E

L

K

A

A

T

R

E

L

H

H

K

R

P

P

K

K

L

L

I

I

M

V

V

A

G

A

A

A

S
|
S

A

A

Y

Y

P

P

R

R

T

I

I

I

D

D

F

F

K

A

K

K

F

F

R

R

E

E

I

I

A

A

D

D

K

E

V

V

G

G

A

A

V

Y

L

L

V

M

V

V

D
|
D

M

M

A
|
A

H
|
H

I

I

A

A

G

G

L

L

V

V

A

A

A

A

G

G

A

L

H

H

P

P

S

N

P

P

V

V

P

P

Y

Y

A

A

D

H

F

F

V

V

T

T

T

T

T
|
T

T

T

H

H

K
|
K

T

T

L

L

R

R

G

G

P

P

R
|
R

G

G

G

G

L

M

I

I

L

L

C

C

K

Q

E

E

E

Q

Y

F

A

A

K

K

T

Q

L

I

N

D

S

K

Q

A

I

I

F

F

P

P

G

G

I

I

Q

Q

G

G

G

G

P

P

L

L

M

M

H

H

V

V

I

I

A

A

A

A

K

K

A

A

V

V

A

A

L

F

K

G

E

E

A

A

M

L

S

Q

E

D

D

D

F

F

K

K

V

A

Y

Y

Q

A

H

K

Q

R

I

V

V

V

K

D

N

N

A

A

K

K

R

R

L

L

S

A

E

S

R

A

L

L

M

Q

K

N

H

E

G

G

F

F

K

T

L

L

V

V

S

S

G

G

G

G

T

T

D

D

N

N

H

H

L

L

M

L

L

L

I

V

N

D

L

L

S

R

N

P

S

Q

E

Q

I

L

T

T

G

G

K

K

E

T

A

A

E

E

E

K

A

V

L

L

G

D

R

E

A

V

N

G

I

I

T

T

V

V

N

N

K

K

N

N

T

T

V

I

P

P

F

Y

E

D

T

P

R

E

S

S

P

P

F

G

V

V

T

T

S

S

G

G

V

I

R
|
R

I

I

G

G

T

T

P

A

A

A

V

V

T

T

T

T

R

R

G

G

M

F

K

G

E

L

S

E

E

E

M

M

D

D

F

E

I

I

G

A

D

A

L

I

I

I

A

G

Q

L

V

V

L

L

S

K

D

N

I

V

K

G

S

S

D

E

L

Q

N

A

I

L

N

E

D

E

V

A

K

R

S

Q

K

R

V

V

L

A

E

A

L

L

C

T

active site: Y51 (= Y54), E53 (= E56), D197 (= D200), T223 (= T226), K226 (= K229), R232 (= R235)
binding allo-threonine: S31 (= S34), H122 (= H125), H200 (= H203), R357 (= R360)
binding pyridoxal-5'-phosphate: S93 (= S96), G94 (= G97), A95 (≠ S98), H122 (= H125), S172 (= S175), D197 (= D200), A199 (= A202), H200 (= H203), T223 (= T226), K226 (= K229)

4ot8A X-ray crystal structure of serine hydroxymethyl transferase from burkholderia cenocepacia bound to plp and serine
65% identity, 98% coverage: 5:414/418 of query aligns to 6:414/414 of 4ot8A

query
sites
4ot8A

N

S

A

T

V

I

R

A

Q

N

A

V

D

D

P

P

E

E

I

I

Y

F

D

A

A

A

L

I

K

E

K

Q

E

E

I

N

E

R

R

R

Q

Q

E

E

T

D

H

H

I

I

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

F

Y

V

T

S

S

P

P

A

A

V

V

L

M

E

A

A

A

Q

Q

G

G

S

S

I

Q

M

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

A

G

K

K

R

R

Y

Y

Y
|
Y

G

G

G

G

C

C

E

E

F

Y

V

V

D

D

I

V

A

V

E

E

E

Q

L

L

A

A

I

I

K

D

R

R

A

V

K

K

E

Q

L

L

F

F

G

G

A

A

E

E

H

A

A

A

N

N

V

V

Q

Q

A

P

H

N

S
|
S

G
|
G

S
|
S

Q

Q

A

A

N

N

M

Q

A

G

V

V

Y

F

F

F

A

A

V

M

L

L

K

K

P

P

G

G

D

D

T

T

I

I

M

M

G

G

M

M

N

S

L

L

S

A

H

H

G

G

G

G

H
|
H

L

L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

F

M

S

S

G

G

K

K

L

W

F

F

N

N

V

V

I

V

S

S

Y

Y

G

G

V

L

N

N

K

-

E

E

T

N

E

E

T

D

I

I

D

D

Y

Y

D

D

E

A

A

A

E

E

K

K

L

L

A

A

T

N

E

E

H

H

K

K

P

P

K

K

L

L

I

I

M

V

V

A

G

G

A

A

S

S

A

A

Y

F

P

A

R

L

T

K

I

I

D

D

F

F

K

E

K

R

F

L

R

A

E

K

I

I

A

A

D

K

K

S

V

V

G

G

A

A

V

Y

L

L

V

M

V

V

D
|
D

M

M

A
|
A

H
|
H

I

Y

A

A

G

G

L

L

V

I

A

A

A

A

G

G

A

V

H

Y

P

P

S

N

P

P

V

V

P

P

Y

H

A

A

D

D

F

F

V

V

T

T

T

T

T
|
T

T

T

H

H

K
|
K

T

S

L

L

R

R

G

G

P

P

R
|
R

G

G

G

G

L

V

I

I

L

L

C

M

K

K

E

A

E

E

Y

Y

A

E

K

K

T

P

L

I

N

N

S

S

Q

A

I

I

F

F

P

P

G

G

I

I

Q

Q

G

G

G

G

P

P

L

L

M

M

H

H

V

V

I

I

A

A

A

A

K

K

A

A

V

V

A

A

L

F

K

K

E

E

A

A

M

L

S

S

E

P

D

E

F

F

K

K

V

E

Y

Y

Q

Q

H

Q

Q

K

I

V

V

V

K

E

N

N

A

A

K

R

R

V

L

L

S

A

E

E

R

T

L

L

M

V

K

K

H

R

G

G

F

L

K

R

L

I

V

V

S

S

G

G

G

R

T

T

D

E

N

S

H

H

L

V

M

M

L

L

I

V

N

D

L

L

S

R

N

A

S

K

E

H

I

I

T

T

G

G

K

K

E

A

A

A

E

E

E

A

A

A

L

L

G

G

R

A

A

A

N

H

I

I

T

T

V

V

N

N

K

K

N

N

T

A

V

I

P

P

F

N

E

D

T

P

R

E

S

K

P

P

F

F

V

V

T

T

S

S

G

G

V

I

R
|
R

I

L

G

G

T

S

P

P

A

A

V

M

T

T

T

T

R

R

G

G

M

F

K

G

E

P

S

A

E

E

M

A

D

E

F

Q

I

V

G

G

D

N

L

L

I

I

A

A

Q

D

V

V

L

L

S

E

D

N

I

P

K

E

S

D

D

A

L

A

N

T

I

I

N

E

D

R

V

V

K

R

S

A

K

Q

V

V

L

A

E

E

L

L

C

T

K

K

Q

R

F

F

P

P

L

V

Y

Y

R

R

active site: Y55 (= Y54), E57 (= E56), D200 (= D200), T226 (= T226), K229 (= K229), R235 (= R235)
binding pyridoxal-5'-phosphate: S97 (= S96), G98 (= G97), S99 (= S98), H126 (= H125), D200 (= D200), A202 (= A202), H203 (= H203), K229 (= K229)
binding serine: S35 (= S34), E57 (= E56), Y65 (= Y64), H126 (= H125), H203 (= H203), R360 (= R360)

4otlA X-ray crystal structure of serine hydroxymethyl transferase from burkholderia cenocepacia bound to plp and glycine
65% identity, 98% coverage: 5:414/418 of query aligns to 1:409/409 of 4otlA

query
sites
4otlA

N

S

A

T

V

I

R

A

Q

N

A

V

D

D

P

P

E

E

I

I

Y

F

D

A

A

A

L

I

K

E

K

Q

E

E

I

N

E

R

R

R

Q

Q

E

E

T

D

H

H

I

I

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

F

Y

V

T

S

S

P

P

A

A

V

V

L

M

E

A

A

A

Q

Q

G

G

S

S

I

Q

M

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

A

G

K

K

R

R

Y

Y

Y
|
Y

G

G

G

G

C

C

E

E

F

Y

V

V

D

D

I

V

A

V

E

E

E

Q

L

L

A

A

I

I

K

D

R

R

A

V

K

K

E

Q

L

L

F

F

G

G

A

A

E

E

H

A

A

A

N

N

V

V

Q

Q

A

P

H

N

S
|
S

G
|
G

S
|
S

Q

Q

A

A

N

N

M

Q

A

G

V

V

Y

F

F

F

A

A

V

M

L

L

K

K

P

P

G

G

D

D

T

T

I

I

M

M

G

G

M

M

N

S

L

L

S

A

H

H

G

G

G

G

H
|
H

L

L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

F

M

S

S

G

G

K

K

L

W

F

F

N

N

V

V

I

V

S

S

Y

Y

G

G

V

L

N

N

K

-

E

E

T

N

E

E

T

D

I

I

D

D

Y

Y

D

D

E

A

A

A

E

E

K

K

L

L

A

A

T

N

E

E

H

H

K

K

P

P

K

K

L

L

I

I

M

V

V

A

G

G

A

A

S
|
S

A

A

Y

F

P

A

R

L

T

K

I

I

D

D

F

F

K

E

K

R

F

L

R

A

E

K

I

I

A

A

D

K

K

S

V

V

G

G

A

A

V

Y

L

L

V

M

V

V

D
|
D

M

M

A
|
A

H
|
H

I

Y

A

A

G

G

L

L

V

I

A

A

A

A

G

G

A

V

H

Y

P

P

S

N

P

P

V

V

P

P

Y

H

A

A

D

D

F

F

V

V

T

T

T

T

T
|
T

T

T

H

H

K
|
K

T

S

L

L

R

R

G

G

P

P

R
|
R

G

G

G

G

L

V

I

I

L

L

C

M

K

K

E

A

E

E

Y

Y

A

E

K

K

T

P

L

I

N

N

S

S

Q

A

I

I

F

F

P

P

G

G

I

I

Q

Q

G

G

G

G

P

P

L

L

M

M

H

H

V

V

I

I

A

A

A

A

K

K

A

A

V

V

A

A

L

F

K

K

E

E

A

A

M

L

S

S

E

P

D

E

F

F

K

K

V

E

Y

Y

Q

Q

H

Q

Q

K

I

V

V

V

K

E

N

N

A

A

K

R

R

V

L

L

S

A

E

E

R

T

L

L

M

V

K

K

H

R

G

G

F

L

K

R

L

I

V

V

S

S

G

G

G

R

T

T

D

E

N

S

H

H

L

V

M

M

L

L

I

V

N

D

L

L

S

R

N

A

S

K

E

H

I

I

T

T

G

G

K

K

E

A

A

A

E

E

E

A

A

A

L

L

G

G

R

A

A

A

N

H

I

I

T

T

V

V

N

N

K

K

N

N

T

A

V

I

P

P

F

N

E

D

T

P

R

E

S

K

P

P

F

F

V

V

T

T

S

S

G

G

V

I

R
|
R

I

L

G

G

T

S

P

P

A

A

V

M

T

T

T

T

R

R

G

G

M

F

K

G

E

P

S

A

E

E

M

A

D

E

F

Q

I

V

G

G

D

N

L

L

I

I

A

A

Q

D

V

V

L

L

S

E

D

N

I

P

K

E

S

D

D

A

L

A

N

T

I

I

N

E

D

R

V

V

K

R

S

A

K

Q

V

V

L

A

E

E

L

L

C

T

K

K

Q

R

F

F

P

P

L

V

Y

Y

R

R

active site: Y50 (= Y54), E52 (= E56), D195 (= D200), T221 (= T226), K224 (= K229), R230 (= R235)
binding glycine: S30 (= S34), Y50 (= Y54), Y60 (= Y64), H121 (= H125), K224 (= K229), R355 (= R360)
binding pyridoxal-5'-phosphate: S92 (= S96), G93 (= G97), S94 (= S98), H121 (= H125), S170 (= S175), D195 (= D200), A197 (= A202), H198 (= H203), K224 (= K229)

4n0wA X-ray crystal structure of a serine hydroxymethyltransferase from burkholderia cenocepacia with covalently attached pyridoxal phosphate
65% identity, 98% coverage: 5:414/418 of query aligns to 8:416/416 of 4n0wA

query
sites
4n0wA

N

S

A

T

V

I

R

A

Q

N

A

V

D

D

P

P

E

E

I

I

Y

F

D

A

A

A

L

I

K

E

K

Q

E

E

I

N

E

R

R

R

Q

Q

E

E

T

D

H

H

I

I

E

E

L

L

I

I

A

A

S

S

E

E

N

N

F

Y

V

T

S

S

P

P

A

A

V

V

L

M

E

A

A

A

Q

Q

G

G

S

S

I

Q

M

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

A

G

K

K

R

R

Y

Y

Y

Y

G

G

G

G

C

C

E

E

F

Y

V

V

D

D

I

V

A

V

E

E

E

Q

L

L

A

A

I

I

K

D

R

R

A

V

K

K

E

Q

L

L

F

F

G

G

A

A

E

E

H

A

A

A

N

N

V

V

Q

Q

A

P

H

N

S
|
S

G
|
G

S
|
S

Q

Q

A

A

N

N

M

Q

A

G

V

V

Y

F

F

F

A

A

V

M

L

L

K

K

P

P

G

G

D

D

T

T

I

I

M

M

G

G

M

M

N

S

L

L

S

A

H

H

G

G

G

G

H
|
H

L

L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

F

M

S

S

G

G

K

K

L

W

F

F

N

N

V

V

I

V

S

S

Y

Y

G

G

V

L

N

N

K

-

E

E

T

N

E

E

T

D

I

I

D

D

Y

Y

D

D

E

A

A

A

E

E

K

K

L

L

A

A

T

N

E

E

H

H

K

K

P

P

K

K

L

L

I

I

M

V

V

A

G

G

A

A

S

S

A

A

Y

F

P

A

R

L

T

K

I

I

D

D

F

F

K

E

K

R

F

L

R

A

E

K

I

I

A

A

D

K

K

S

V

V

G

G

A

A

V

Y

L

L

V

M

V

V

D
|
D

M

M

A
|
A

H
|
H

I

Y

A

A

G

G

L

L

V

I

A

A

A

A

G

G

A

V

H

Y

P

P

S

N

P

P

V

V

P

P

Y

H

A

A

D

D

F

F

V

V

T

T

T

T

T
|
T

T

T

H

H

K
|
K

T

S

L

L

R

R

G

G

P

P

R
|
R

G

G

G

G

L

V

I

I

L

L

C

M

K

K

E

A

E

E

Y

Y

A

E

K

K

T

P

L

I

N

N

S

S

Q

A

I

I

F

F

P

P

G

G

I

I

Q

Q

G

G

G

G

P

P

L

L

M

M

H

H

V

V

I

I

A

A

A

A

K

K

A

A

V

V

A

A

L

F

K

K

E

E

A

A

M

L

S

S

E

P

D

E

F

F

K

K

V

E

Y

Y

Q

Q

H

Q

Q

K

I

V

V

V

K

E

N

N

A

A

K

R

R

V

L

L

S

A

E

E

R

T

L

L

M

V

K

K

H

R

G

G

F

L

K

R

L

I

V

V

S

S

G

G

G

R

T

T

D

E

N

S

H

H

L

V

M

M

L

L

I

V

N

D

L

L

S

R

N

A

S

K

E

H

I

I

T

T

G

G

K

K

E

A

A

A

E

E

E

A

A

A

L

L

G

G

R

A

A

A

N

H

I

I

T

T

V

V

N

N

K

K

N

N

T

A

V

I

P

P

F

N

E

D

T

P

R

E

S

K

P

P

F

F

V

V

T

T

S

S

G

G

V

I

R

R

I

L

G

G

T

S

P

P

A

A

V

M

T

T

T

T

R

R

G

G

M

F

K

G

E

P

S

A

E

E

M

A

D

E

F

Q

I

V

G

G

D

N

L

L

I

I

A

A

Q

D

V

V

L

L

S

E

D

N

I

P

K

E

S

D

D

A

L

A

N

T

I

I

N

E

D

R

V

V

K

R

S

A

K

Q

V

V

L

A

E

E

L

L

C

T

K

K

Q

R

F

F

P

P

L

V

Y

Y

R

R

active site: Y57 (= Y54), E59 (= E56), D202 (= D200), T228 (= T226), K231 (= K229), R237 (= R235)
binding pyridoxal-5'-phosphate: S99 (= S96), G100 (= G97), S101 (= S98), H128 (= H125), D202 (= D200), A204 (= A202), H205 (= H203), K231 (= K229)

3pgyB Serine hydroxymethyltransferase from staphylococcus aureus, s95p mutant.
63% identity, 97% coverage: 7:413/418 of query aligns to 4:404/404 of 3pgyB

query
sites
3pgyB

V

I

R

T

Q

K

A

Q

D

D

P

K

E

V

I

I

Y

A

D

E

A

A

L

I

K

E

K

R

E

E

I

F

E

Q

R

R

Q

Q

E

N

T

S

H

N

I

I

E

E

L

L

I

I

A

A

S

S

E

E

N

N

F

F

V

V

S

S

P

E

A

A

V

V

L

M

E

E

A

A

Q

Q

G

G

S

S

I

V

M

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

A

G

K

R

R

R

Y

Y

Y

Y

G

G

G

G

C

C

E

E

F

F

V

V

D

D

I

V

A

T

E

E

E

S

L

I

A

A

I

I

K

D

R

R

A

A

K

K

E

A

L

L

F

F

G

G

A

A

E

E

H

H

A

V

N

N

V

V

Q

Q

A

P

H

H

S

S

G

G

S

P

Q

Q

A

A

N

N

M

M

A

A

V

V

Y

Y

F

L

A

V

V

A

L

L

K

E

P

M

G

G

D

D

T

T

I

V

M

L

G

G

M

M

N

N

L

L

S

S

H

H

G

-

G

-

H

-

L

-

T

-

H

-

G

-

S

A

P

P

V

V

N

N

F

F

S

S

G

G

K

K

L

F

F

Y

N

N

V

F

I

V

S

E

Y

Y

G

G

V

V

N

D

K

K

E

D

T

T

E

E

T

R

I

I

D

N

Y

Y

D

D

E

E

A

V

E

R

K

K

L

L

A

A

T

L

E

E

H

H

K

K

P

P

K

K

L

L

I

I

M

V

V

A

G

G

A

A

S

S

A

A

Y

Y

P

S

R

R

T

T

I

I

D

D

F

F

K

K

K

K

F

F

R

K

E

E

I

I

A

A

D

D

K

E

V

V

G

N

A

A

V

K

L

L

V

M

V

V

D
|
D

M

M

A

A

H

H

I

I

A

A

G

G

L

L

V

V

A

A

A

A

G

G

A

L

H

H

P

P

S

N

P

P

V

V

P

E

Y

Y

A

A

D

D

F

F

V

V

T

T

T

T

T
|
T

T

T

H

H

K
|
K

T

T

L

L

R

R

G

G

P

P

R
|
R

G

G

G

G

L

M

I

I

L

L

C

C

K

K

E

E

E

E

Y

Y

A

K

K

K

T

D

L

I

N

D

S

K

Q

T

I

I

F

F

P

P

G

G

I

I

Q

Q

G

G

G

G

P

P

L

L

M

E

H

H

V

V

I

I

A

A

A

A

K

K

A

A

V

V

A

A

L

F

K

G

E

E

A

A

M

L

S

E

E

N

D

N

F

F

K

K

V

T

Y

Y

Q

Q

H

Q

Q

Q

I

V

V

V

K

K

N

N

A

A

K

K

R

V

L

L

S

A

E

E

R

A

L

L

M

I

K

N

H

E

G

G

F

F

K

R

L

I

V

V

S

S

G

G

G

G

T

T

D

D

N

N

H

H

L

L

M

V

L

A

I

V

N

D

L

V

S

K

N

G

S

S

-

I

E

G

I

L

T

T

G

G

K

K

E

E

A

A

E

E

E

E

A

T

L

L

G

D

R
x
S

A

V

N

G

I

I

T

T

V

C

N

N

K

K

N

N

T

T

V

I

P

P

F

F

E

D

T

Q

R

E

S

K

P

P

F

F

V

V

T

T

S

S

G

G

V

I

R

R

I

L

G

G

T

T

P

P

A

A

V

A

T

T

T

T

R

R

G

G

M

F

K

D

E

E

S

K

E

A

M

F

D

E

F

E

I

V

G

A

D

K

L

I

I

I

A

S

Q

L

V

A

L

L

S

K

D

N

I

S

K

K

S

D

D
x
E

L

E

N

K

I
x
L

N

Q

D

Q

V

A

K

K

S

E

K

R

V

V

L

A

E

K

L

L

C

T

K

A

Q

E

F

Y

P

P

L
|
L

Y
|
Y

active site: Y51 (= Y54), E53 (= E56), D190 (= D200), T216 (= T226), K219 (= K229), R225 (= R235)
binding glycine: S327 (≠ R336), E384 (≠ D393), L387 (≠ I396), L403 (= L412), Y404 (= Y413)

1dfoB Crystal structure at 2.4 angstrom resolution of e. Coli serine hydroxymethyltransferase in complex with glycine and 5-formyl tetrahydrofolate (see paper)
64% identity, 96% coverage: 11:413/418 of query aligns to 12:416/417 of 1dfoB

query
sites
1dfoB

D

D

P

A

E

E

I

L

Y

W

D

Q

A

A

L

M

K

E

K

Q

E

E

I

K

E

V

R

R

Q

Q

E

E

T

E

H

H

I

I

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

F

Y

V

T

S

S

P

P

A

R

V

V

L

M

E

Q

A

A

Q

Q

G

G

S

S

I

Q

M

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

A

G

K

K

R

R

Y
|
Y

Y
|
Y

G

G

G

G

C

C

E

E

F

Y

V

V

D

D

I

I

A

V

E

E

E

Q

L

L

A

A

I

I

K

D

R

R

A

A

K

K

E

E

L

L

F

F

G

G

A

A

E

D

H

Y

A

A

N

N

V

V

Q

Q

A

P

H

H

S
|
S

G
|
G

S
|
S

Q

Q

A

A

N

N

M

F

A

A

V

V

Y

Y

F

T

A

A

V

L

L

L

K

E

P

P

G

G

D

D

T

T

I

V

M

L

G

G

M

M

N

N

L
|
L

S

A

H

H

G

G

G
|
G

H
|
H

L
|
L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

F

F

S

S

G

G

K

K

L

L

F

Y

N

N

V

I

I

V

S

P

Y

Y

G

G

V

I

N

D

K

A

E

-

T

T

E

G

T

H

I

I

D

D

Y

Y

D

A

E

D

A

L

E

E

K

K

L

Q

A

A

T

K

E

E

H

H

K

K

P

P

K

K

L

M

I

I

M

I

V

G

G

G

A
x
F

S
|
S

A

A

Y

Y

P

S

R

G

T

V

I

V

D

D

F

W

K

A

K

K

F

M

R

R

E

E

I

I

A

A

D

D

K

S

V

I

G

G

A

A

V

Y

L

L

V

F

V

V

D
|
D

M

M

A
|
A

H
|
H

I

V

A

A

G

G

L

L

V

V

A

A

A

A

G

G

A

V

H

Y

P

P

S

N

P

P

V

V

P

P

Y

H

A

A

D

H

F

V

V

V

T

T

T

T

T
|
T

T

T

H

H

K
|
K

T

T

L

L

R

A

G

G

P

P

R
|
R

G

G

G

G

L

L

I

I

L

L

C

A

K

K

-

G

-

G

-
x
S

E

E

E
|
E

Y

L

A

Y

K

K

T

K

L

L

N

N

S

S

Q

A

I

V

F

F

P

P

G

G

I

G

Q

Q

G
|
G

G

G

P

P

L

L

M

M

H

H

V

V

I

I

A

A

A

G

K

K

A

A

V

V

A

A

L

L

K

K

E

E

A

A

M

M

S

E

E

P

D

E

F

F

K

K

V

T

Y

Y

Q

Q

H

Q

Q

Q

I

V

V

A

K

K

N