SitesBLAST

4n0wA X-ray crystal structure of a serine hydroxymethyltransferase from burkholderia cenocepacia with covalently attached pyridoxal phosphate
65% identity, 98% coverage: 5:414/418 of query aligns to 8:416/416 of 4n0wA

query
sites
4n0wA

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active site: Y57 (= Y54), E59 (= E56), D202 (= D200), T228 (= T226), K231 (= K229), R237 (= R235)
binding pyridoxal-5'-phosphate: S99 (= S96), G100 (= G97), S101 (= S98), H128 (= H125), D202 (= D200), A204 (= A202), H205 (= H203), K231 (= K229)

3pgyB Serine hydroxymethyltransferase from staphylococcus aureus, s95p mutant.
63% identity, 97% coverage: 7:413/418 of query aligns to 4:404/404 of 3pgyB

query
sites
3pgyB

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active site: Y51 (= Y54), E53 (= E56), D190 (= D200), T216 (= T226), K219 (= K229), R225 (= R235)
binding glycine: S327 (≠ R336), E384 (≠ D393), L387 (≠ I396), L403 (= L412), Y404 (= Y413)

1dfoB Crystal structure at 2.4 angstrom resolution of e. Coli serine hydroxymethyltransferase in complex with glycine and 5-formyl tetrahydrofolate (see paper)
64% identity, 96% coverage: 11:413/418 of query aligns to 12:416/417 of 1dfoB

query
sites
1dfoB

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active site: Y55 (= Y54), E57 (= E56), D200 (= D200), T226 (= T226), K229 (= K229), R235 (= R235)
binding N-[4-({[(6S)-2-amino-5-formyl-4-oxo-3,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)benzoyl]-L-glutamic acid: E57 (= E56), Y64 (= Y63), Y65 (= Y64), L121 (= L120), G125 (= G124), H126 (= H125), L127 (= L126), S175 (= S175), S245 (vs. gap), E247 (= E244), N347 (= N344), S355 (= S352), P356 (= P353), F357 (= F354)
binding n-glycine-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-yl-methane]: S35 (= S34), Y55 (= Y54), Y65 (= Y64), S97 (= S96), G98 (= G97), S99 (= S98), H126 (= H125), F174 (≠ A174), S175 (= S175), D200 (= D200), A202 (= A202), H203 (= H203), K229 (= K229), G262 (= G259), R363 (= R360)

P0A825 Serine hydroxymethyltransferase; SHMT; Serine methylase; EC 2.1.2.1 from Escherichia coli (strain K12) (see 8 papers)
64% identity, 96% coverage: 11:413/418 of query aligns to 12:416/417 of P0A825

query
sites
P0A825

D

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K

E

K

Q

E

E

I

K

E

V

R

R

Q

Q

E

E

T

E

H

H

I

I

E

E

L

L

I

I

A

A

S

S

E

E

N

N

F

Y

V

T

S

S

P

P

A

R

V

V

L

M

E

Q

A

A

Q

Q

G

G

S

S

I

Q

M

L

T

T

N

N

K
|
K

Y
|
Y

A

A

E

E

G

G

Y

Y

P

P

A

G

K
|
K

R

R

Y

Y

Y
|
Y

G

G

C

C

E

E

F

Y

V

V

D

D

I

I

A

V

E

E

E

Q

L

L

A

A

I

I

K

D

R

R

A

A

K

K

E

E

L
|
L

F

F

G

G

A

A

E

D

H

Y

A

A

N

N

V

V

Q

Q

A

P

H

H

S

S

G

G

S

S

Q

Q

A

A

N

N

M

F

A

A

V

V

Y

Y

F

T

A

A

V

L

L

L

K

E

P

P

G

G

D

D

T

T

I

V

M

L

G

G

M

M

N

N

L

L

S

A

H

H

G

G

H

H

L

L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

F

F

S

S

G

G

K

K

L

L

F

Y

N

N

V

I

I

V

S

P

Y

Y

G

G

V

I

N

D

K

A

E

-

T

T

E

G

T

H

I

I

D

D

Y

Y

D

A

E

D

A

L

E

E

K

K

L

Q

A

A

T

K

E

E

H

H

K

K

P

P

K

K

L

M

I

I

M

I

V

G

G

G

A

F

S

S

A

A

Y

Y

P

S

R

G

T

V

I

V

D

D

F

W

K

A

K

K

F

M

R

R

E

E

I

I

A

A

D

D

K

S

V

I

G

G

A

A

V

Y

L

L

V

F

V

V

D

D

M

M

A

A

H

H

I

V

A

A

G

G

L

L

V

V

A

A

G

G

A

V

H

Y

P
|
P

S

N

P
|
P

V

V

P
|
P

Y

H

A

A

D

H

F

V

V

V

T

T

H
|
H

K
|
K

T

T

L

L

R

A

G

G

P

P

R
|
R

G

G

L

L

I

I

L

L

C

A

K
|
K

-

G

-

S

E

E

Y

L

A

Y

K
|
K

T

K

L

L

N

N

S

S

Q

A

I

V

F

F

P
|
P

G

G

I

G

Q

Q

G

G

P
|
P

L

L

M

M

H

H

V

V

I

I

A

A

A

G

K

K

A

A

V

V

A

A

L
|
L

K
|
K

E

E

A

A

M

M

S

E

E

P

D

E

F

F

K
|
K

V

T

Y

Y

Q

Q

H

Q

Q

Q

I

V

V

A

K
|
K

N

N

A

A

K

K

R

A

L

M

S

V

E

E

R

V

L

F

M

L

K

E

H

R

G

G

F

Y

K

K

L

V

V

V

S

S

G

G

T

T

D

D

N

N

H

H

L

L

M

F

L

L

I

V

N

D

L

L

S

V

N

D

S

K

E

N

I

L

T

T

G

G

K
|
K

E

E

A

A

E

D

E

A

A

A

L

L

G

G

R

R

A

A

N

N

I

I

T

T

V

V

N

N

K
|
K

N

N

T

S

V

V

P

P

F

N

E

D

T

P

R
x
K

S

S

P

P

F

F

V

V

T

T

S

S

G

G

V

I

R
|
R

I

V

G

G

T

T

P

P

A

A

V

I

T

T

T

R

R
|
R

G

G

M

F

K
|
K

E

E

S

A

E

E

M

A

D

K

F

E

I

L

G

A

D

G

L

W

I

M

A

C

Q

D

V

V

L

L

S

D

D

S

I

I

K

N

S

D

D

E

L

A

N

V

I

I

N

E

D

R

V

I

K

K

S

G

K

K

V

V

L

L

E

D

L

I

C

C

K

A

Q

R

F

Y

P

P

L

V

Y

Y

K54 (= K53) modified: N6-acetyllysine
Y55 (= Y54) mutation to F: 50 and 15-fold increase in the affinity for serine and tetrahydrofolate, respectively, and 4-fold decrease in the catalytic efficiency.
K62 (= K61) modified: N6-succinyllysine
Y65 (= Y64) mutation to F: Decrease in catalytic activity.
L85 (= L84) mutation to A: Alteration of the dimer-monomer equilibrium accompanied by minor changes in the catalytic properties and whitout any significant change of tertiary structure. In the monomeric state; when associated with A-276.
P214 (= P214) mutation to A: No significant difference in catalytic efficiency and affinity compared to the wild-type.; mutation to G: No significant difference in catalytic efficiency and affinity compared to the wild-type.
P216 (= P216) mutation to A: No significant difference in catalytic efficiency and affinity compared to the wild-type. Alteration in the folding rate.; mutation to G: Important decrease in affinity and catalytic efficiency. Severely compromised in folding into a catalytically competent enzyme.
P218 (= P218) mutation to A: No significant difference in catalytic efficiency and affinity compared to the wild-type.; mutation to G: No significant difference in catalytic efficiency and affinity compared to the wild-type.
H228 (= H228) Plays an important role in substrate specificity; binding pyridoxal 5'-phosphate; mutation H->D,N: Utilize substrates and substrate analogs more effectively for a variety of alternate non-physiological reactions.
K229 (= K229) modified: N6-(pyridoxal phosphate)lysine
R235 (= R235) binding pyridoxal 5'-phosphate; mutation to K: 1500- and 20-fold increase in the affinity for serine and tetrahydrofolate, respectively, and 15-fold decrease in the catalytic efficiency.; mutation to L: 450- and 11-fold increase in the affinity for serine and tetrahydrofolate, respectively, and 60-fold decrease in the catalytic efficiency.; mutation to Q: 900- and 17-fold increase in the affinity for serine and tetrahydrofolate, respectively, and 30-fold decrease in the catalytic efficiency.
K242 (= K242) modified: N6-succinyllysine
K250 (= K247) modified: N6-acetyllysine; alternate; modified: N6-succinyllysine; alternate
P258 (= P255) mutation to A: Important decrease in affinity and catalytic efficiency. Reduced thermal stability.; mutation to G: Important decrease in affinity and catalytic efficiency.
P264 (= P261) mutation to A: Important decrease in affinity and catalytic efficiency.; mutation to G: Important decrease in affinity and catalytic efficiency.
L276 (= L273) mutation to A: Alteration of the dimer-monomer equilibrium accompanied by minor changes in the catalytic properties and whitout any significant change of tertiary structure. In the monomeric state; when associated with A-85.
K277 (= K274) modified: N6-succinyllysine
K285 (= K282) modified: N6-acetyllysine
K293 (= K290) modified: N6-succinyllysine
K331 (= K328) modified: N6-succinyllysine
K346 (= K343) modified: N6-succinyllysine
K354 (≠ R351) modified: N6-acetyllysine; alternate; modified: N6-succinyllysine; alternate
R363 (= R360) mutation to A: It does not bind serine and glycine and shows no activity with serine as the substrate.; mutation to K: Exhibits only 0.03% of the catalytic activity of the wild-type and a 15-fold reduction in affinity for glycine and serine.
R372 (= R369) mutation to A: No significant difference compared to the wild-type.; mutation to K: No significant difference compared to the wild-type.
K375 (= K372) modified: N6-acetyllysine

1eqbA X-ray crystal structure at 2.7 angstroms resolution of ternary complex between the y65f mutant of e-coli serine hydroxymethyltransferase, glycine and 5-formyl tetrahydrofolate (see paper)
64% identity, 96% coverage: 11:413/418 of query aligns to 11:415/416 of 1eqbA

query
sites
1eqbA

D

D

P

A

E

E

I

L

Y

W

D

Q

A

A

L

M

K

E

K

Q

E

E

I

K

E

V

R

R

Q

Q

E

E

T

E

H

H

I

I

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

F

Y

V

T

S

S

P

P

A

R

V

V

L

M

E

Q

A

A

Q

Q

G

G

S

S

I

Q

M

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

A

G

K

K

R

R

Y
|
Y

Y
x
F

G

G

C

C

E

E

F

Y

V

V

D

D

I

I

A

V

E

E

E

Q

L

L

A

A

I

I

K

D

R

R

A

A

K

K

E

E

L

L

F

F

G

G

A

A

E

D

H

Y

A

A

N

N

V

V

Q

Q

A

P

H

H

S
|
S

G
|
G

S
|
S

Q

Q

A

A

N

N

M

F

A

A

V

V

Y

Y

F

T

A

A

V

L

L

L

K

E

P

P

G

G

D

D

T

T

I

V

M

L

G

G

M

M

N

N

L
|
L

S

A

H

H

G
|
G

H
|
H

L
|
L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

F

F

S

S

G

G

K

K

L

L

F

Y

N

N

V

I

I

V

S

P

Y

Y

G

G

V

I

N

D

K

A

E

-

T

T

E

G

T

H

I

I

D

D

Y

Y

D

A

E

D

A

L

E

E

K

K

L

Q

A

A

T

K

E

E

H

H

K

K

P

P

K

K

L

M

I

I

M

I

V

G

G

G

A
x
F

S
|
S

A

A

Y

Y

P

S

R

G

T

V

I

V

D

D

F

W

K

A

K

K

F

M

R

R

E

E

I

I

A

A

D

D

K

S

V

I

G

G

A

A

V

Y

L

L

V

F

V

V

D
|
D

M

M

A

A

H
|
H

I

V

A

A

G

G

L

L

V

V

A

A

G

G

A

V

H

Y

P

P

S

N

P

P

V

V

P

P

Y

H

A

A

D

H

F

V

V

V

T

T

T
|
T

T

T

H
|
H

K
|
K

T

T

L

L

R

A

G

G

P

P

R
|
R

G

G

L

L

I

I

L

L

C

A

K

K

-

G

-

S

E

E

Y

L

A

Y

K

K

T

K

L

L

N

N

S

S

Q

A

I

V

F

F

P

P

G

G

I

G

Q

Q

G

G

P

P

L

L

M

M

H

H

V

V

I

I

A

A

A

G

K

K

A

A

V

V

A

A

L

L

K

K

E

E

A

A

M

M

S

E

E

P

D

E

F

F

K

K

V

T

Y

Y

Q

Q

H

Q

Q

Q

I

V

V

A

K

K

N

N

A

A

K

K

R

A

L

M

S

V

E

E

R

V

L

F

M

L

K

E

H

R

G

G

F

Y

K

K

L

V

V

V

S

S

G

G

T

T

D

D

N

N

H

H

L

L

M

F

L

L

I

V

N

D

L

L

S

V

N

D

S

K

E

N

I

L

T

T

G

G

K

K

E

E

A

A

E

D

E

A

A

A

L

L

G

G

R

R

A

A

N

N

I

I

T

T

V

V

N

N

K

K

N
|
N

T

S

V

V

P

P

F

N

E

D

T

P

R

K

S
|
S

P
|
P

F
|
F

V

V

T

T

S

S

G

G

V

I

R
|
R

I

V

G

G

T

T

P

P

A

A

V

I

T

T

T

R

R

R

G

G

M

F

K

K

E

E

S

A

E

E

M

A

D

K

F

E

I

L

G

A

D

G

L

W

I

M

A

C

Q

D

V

V

L

L

S

D

D

S

I

I

K

N

S

D

D

E

L

A

N

V

I

I

N

E

D

R

V

I

K

K

S

G

K

K

V

V

L

L

E

D

L

I

C

C

K

A

Q

R

F

Y

P

P

L

V

Y

Y

active site: Y54 (= Y54), E56 (= E56), D199 (= D200), T225 (= T226), K228 (= K229), R234 (= R235)
binding N-[4-({[(6S)-2-amino-5-formyl-4-oxo-3,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)benzoyl]-L-glutamic acid: E56 (= E56), Y63 (= Y63), L120 (= L120), G123 (= G123), G124 (= G124), H125 (= H125), L126 (= L126), S174 (= S175), N346 (= N344), S354 (= S352), P355 (= P353), F356 (= F354)
binding glycine: S34 (= S34), Y54 (= Y54), F64 (≠ Y64), H202 (= H203), K228 (= K229), R362 (= R360)
binding pyridoxal-5'-phosphate: S96 (= S96), G97 (= G97), S98 (= S98), H125 (= H125), F173 (≠ A174), S174 (= S175), D199 (= D200), H202 (= H203), H227 (= H228), K228 (= K229)

7x5oB Crystal structure of e. Faecium shmt in complex with me-thf and plp- gly (see paper)
64% identity, 97% coverage: 11:414/418 of query aligns to 7:412/412 of 7x5oB

query
sites
7x5oB

D

D

P

P

E

D

I

L

Y

W

D

A

A

A

L

I

K

A

K

K

E

E

I

E

E

E

R

R

Q

Q

E

E

T

H

H

N

I

L

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

F

F

V

V

S

S

P

E

A

A

V

V

L

M

E

A

A

A

Q

Q

G

G

S

S

I

I

M

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

A

G

K

H

R

R

Y
|
Y

G

G

C

C

E

E

F

F

V

V

D

D

I

I

A

V

E

E

E

N

L

L

A

A

I

I

K

D

R

R

A

A

K

K

E

E

L

L

F

F

G

G

A

A

E

K

H

F

A

A

N

N

V

V

Q

Q

A

P

H

H

S
|
S

G
|
G

S
|
S

Q

Q

A

A

N

N

M

T

A

A

V

A

Y

Y

F

L

A

A

V

L

L

V

K

E

P

P

G

G

D

D

T

T

I

I

M

L

G

G

M

M

N

D

L
|
L

S

S

H

A

G
|
G

H
|
H

L

L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

F

F

S

S

G

G

K

K

L

T

F

Y

N

H

V

F

I

V

S

A

Y

Y

G

G

V

V

N

D

K

P

E

T

T

T

E

E

T

V

I

I

D

D

Y

Y

D

N

E

V

A

V

E

R

K

I

L

L

A

A

T

R

E

K

H

H

K

Q

P

P

K

K

L

L

I

I

M

V

V

A

G

G

A

A

S
|
S

A

A

Y

Y

P

G

R

R

T

T

I

I

D

D

F

F

K

A

K

K

F

F

R

R

E

E

I

I

A

A

D

D

K

E

V

V

G

G

A

A

V

K

L

L

V

M

V

V

D
|
D

M

M

A
|
A

H
|
H

I

I

A

A

G

G

L

L

V

V

A

A

G

G

A

L

H

H

P

P

S

N

P

P

V

V

P

P

Y

Y

A

A

D

D

F

I

V

T

T

T

H

H

K
|
K

T

T

L

L

R

R

G

G

P

P

R

R

G

G

L

M

I

I

L

L

C

T

K

N

E

D

E

E

-

A

Y

L

A

A

K

K

T

K

L

I

N

N

S

S

Q

A

I

V

F

F

P
|
P

G

G

I

I

Q

Q

G

G

P

P

L

L

M

E

H

H

V

V

I

I

A

A

A

G

K

K

A

A

V

V

A

A

L

F

K

K

E

E

A

A

M

L

S

D

E

P

D

A

F

F

K

K

V

E

Y

Y

Q

S

H

E

Q

Q

I

I

V

I

K

A

N

N

A

A

K

K

R

A

L

M

S

V

E

K

R

V

L

F

M

N

K

Q

H

A

-

I

G

G

F

T

K

R

L

V

V

I

S

S

G

G

G

A

T

T

D

D

N

N

H

H

L

L

M

M

L

L

I

I

N

D

L

V

S

R

N

E

S

L

E

G

I

I

T

N

G

G

K

K

E

E

A

A

E

E

E

S

A

I

L

L

G

D

R

S

A

V

N

N

I

I

T

T

V

V

N

N

K

K

N
|
N

T

S

V

I

P

P

F

F

E

E

T

T

R

L

S

S

P
|
P

F

F

V

K

T

T

S

S

G

G

V

I

R
|
R

I

I

G

G

T

T

P

P

A

A

V

I

T

T

R

R

G

G

M

F

K

K

E

E

S

E

E

D

M

A

D

V

F

K

I

V

G

A

D

E

L

L

I

V

A

V

Q

K

V

A

L

L

S

Q

D

A

I

K

K

D

S

D

D

N

L

A

N

Q

I

L

N

D

D

E

V

V

K

K

S

T

K

G

V

V

L

R

E

E

L

L

C

T

K

E

Q

K

F

F

P

P

L

L

Y

H

R

K

binding n-glycine-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-yl-methane]: S30 (= S34), Y50 (= Y54), Y60 (= Y64), S92 (= S96), G93 (= G97), S94 (= S98), H121 (= H125), S171 (= S175), D196 (= D200), A198 (= A202), H199 (= H203), K225 (= K229), R358 (= R360)
binding n-[4-({[(6s)-2-amino-4-hydroxy-5-methyl-5,6,7,8-tetrahydropteridin-6-yl]methyl}amino)benzoyl]-l-glutamic acid: E52 (= E56), Y59 (= Y63), L116 (= L120), G119 (= G123), G120 (= G124), H121 (= H125), S171 (= S175), P252 (= P255), N342 (= N344), P351 (= P353)

7x5nA Crystal structure of e. Faecium shmt in complex with (+)-shin-1 and plp-ser (see paper)
64% identity, 96% coverage: 11:412/418 of query aligns to 6:409/409 of 7x5nA

query
sites
7x5nA

D

D

P

P

E

D

I

L

Y

W

D

A

A

A

L

I

K

A

K

K

E

E

I

E

E

E

R

R

Q

Q

E

E

T

H

H

N

I

L

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

F

F

V

V

S

S

P

E

A

A

V

V

L

M

E

A

A

A

Q

Q

G

G

S

S

I

I

M

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

A

G

K

H

R

R

Y
|
Y

G

G

C

C

E

E

F

F

V

V

D

D

I

I

A

V

E

E

E

N

L

L

A

A

I

I

K

D

R

R

A

A

K

K

E

E

L

L

F

F

G

G

A

A

E

K

H

F

A

A

N

N

V

V

Q

Q

A

P

H

H

S
|
S

G
|
G

S
|
S

Q

Q

A

A

N

N

M

T

A

A

V

A

Y

Y

F

L

A

A

V

L

L

V

K

E

P

P

G

G

D

D

T

T

I

I

M

L

G

G

M

M

N

D

L
|
L

S

S

H

A

G

G

G
|
G

H
|
H

L
|
L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

F

F

S

S

G

G

K

K

L

T

F

Y

N

H

V

F

I

V

S

A

Y

Y

G

G

V

V

N

D

K

P

E

T

T

T

E

E

T

V

I

I

D

D

Y

Y

D

N

E

V

A

V

E

R

K

I

L

L

A

A

T

R

E

K

H

H

K

Q

P

P

K

K

L

L

I

I

M

V

V

A

G

G

A

A

S
|
S

A

A

Y

Y

P

G

R

R

T

T

I

I

D

D

F

F

K

A

K

K

F

F

R

R

E

E

I

I

A

A

D

D

K

E

V

V

G

G

A

A

V

K

L

L

V

M

V

V

D
|
D

M

M

A
|
A

H
|
H

I

I

A

A

G

G

L

L

V

V

A

A

G

G

A

L

H

H

P

P

S

N

P

P

V

V

P

P

Y

Y

A

A

D

D

F

I

V

T

T

T

H

H

K
|
K

T

T

L

L

R

R

G

G

P

P

R

R

G

G

L

M

I

I

L

L

C

T

K

N

E

D

E

E

-

A

Y

L

A

A

K

K

T

K

L

I

N

N

S

S

Q

A

I

V

F

F

P

P

G

G

I

I

Q

Q

G

G

P

P

L

L

M

E

H

H

V

V

I

I

A

A

A

G

K

K

A

A

V

V

A

A

L

F

K

K

E

E

A

A

M

L

S

D

E

P

D

A

F

F

K

K

V

E

Y

Y

Q

S

H

E

Q

Q

I

I

V

I

K

A

N

N

A

A

K

K

R

A

L

M

S

V

E

K

R

V

L

F

M

N

K

Q

H

A

-

I

G

G

F

T

K

R

L

V

V

I

S

S

G

G

G

A

T

T

D

D

N

N

H

H

L

L

M

M

L

L

I

I

N

D

L

V

S

R

N

E

S

L

E

G

I

I

T

N

G

G

K

K

E

E

A

A

E

E

E

S

A

I

L

L

G

D

R

S

A

V

N

N

I

I

T

T

V

V

N

N

K
|
K

N
|
N

T
x
S

V

I

P

P

F

F

E

E

T

T

R

L

S

S

P
|
P

F
|
F

V

K

T

T

S

S

G

G

V

I

R
|
R

I

I

G

G

T

T

P

P

A

A

V

I

T

T

R

R

G

G

M

F

K

K

E

E

S

E

E

D

M

A

D

V

F

K

I

V

G

A

D

E

L

L

I

V

A

V

Q

K

V

A

L

L

S

Q

D

A

I

K

K

D

S

D

D

N

L

A

N

Q

I

L

N

D

D

E

V

V

K

K

S

T

K

G

V

V

L

R

E

E

L

L

C

T

K

E

Q

K

F

F

P

P

L

L

binding (4R)-6-azanyl-4-[3-(hydroxymethyl)-5-phenyl-phenyl]-3-methyl-4-propan-2-yl-1H-pyrano[2,3-c]pyrazole-5-carbonitrile: E51 (= E56), Y58 (= Y63), Y59 (= Y64), L115 (= L120), G119 (= G124), H120 (= H125), L121 (= L126), K340 (= K343), N341 (= N344), S342 (≠ T345), P350 (= P353), F351 (= F354), R357 (= R360)
binding [3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-serine: S29 (= S34), Y49 (= Y54), E51 (= E56), Y59 (= Y64), S91 (= S96), G92 (= G97), S93 (= S98), H120 (= H125), S170 (= S175), D195 (= D200), A197 (= A202), H198 (= H203), K224 (= K229), R357 (= R360)

7v3dA Complex structure of serine hydroxymethyltransferase from enterococcus faecium and its inhibitor (see paper)
64% identity, 96% coverage: 11:412/418 of query aligns to 6:409/409 of 7v3dA

query
sites
7v3dA

D

D

P

P

E

D

I

L

Y

W

D

A

A

A

L

I

K

A

K

K

E

E

I

E

E

E

R

R

Q

Q

E

E

T

H

H

N

I

L

E

E

L

L

I

I

A

A

S

S

E

E

N

N

F

F

V

V

S

S

P

E

A

A

V

V

L

M

E

A

A

A

Q

Q

G

G

S

S

I

I

M

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

A

G

K

H

R

R

Y
|
Y

Y

Y

G

G

C

C

E

E

F

F

V

V

D

D

I

I

A

V

E

E

E

N

L

L

A

A

I

I

K

D

R

R

A

A

K

K

E

E

L

L

F

F

G

G

A

A

E

K

H

F

A

A

N

N

V

V

Q

Q

A

P

H

H

S
|
S

G
|
G

S
|
S

Q

Q

A

A

N

N

M

T

A

A

V

A

Y

Y

F

L

A

A

V

L

L

V

K

E

P

P

G

G

D

D

T

T

I

I

M

L

G

G

M

M

N

D

L
|
L

S

S

H

A

G

G

G
|
G

H
|
H

L
|
L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

F

F

S

S

G

G

K

K

L

T

F

Y

N

H

V

F

I

V

S

A

Y

Y

G

G

V

V

N

D

K

P

E

T

T

T

E

E

T

V

I

I

D

D

Y

Y

D

N

E

V

A

V

E

R

K

I

L

L

A

A

T

R

E

K

H

H

K

Q

P

P

K

K

L

L

I

I

M

V

V

A

G

G

A

A

S
|
S

A

A

Y

Y

P

G

R

R

T

T

I

I

D

D

F

F

K

A

K

K

F

F

R

R

E

E

I

I

A

A

D

D

K

E

V

V

G

G

A

A

V

K

L

L

V

M

V

V

D
|
D

M

M

A
|
A

H

H

I

I

A

A

G

G

L

L

V

V

A

A

G

G

A

L

H

H

P

P

S

N

P

P

V

V

P

P

Y

Y

A

A

D

D

F

I

V

T

T

T

H

H

K
|
K

T

T

L

L

R

R

G

G

P

P

R

R

G

G

L

M

I

I

L

L

C

T

K

N

E

D

E

E

-

A

Y

L

A

A

K

K

T

K

L

I

N

N

S

S

Q

A

I

V

F

F

P

P

G

G

I

I

Q

Q

G
|
G

G

G

P

P

L

L

M

E

H

H

V

V

I

I

A

A

A

G

K

K

A

A

V

V

A

A

L

F

K

K

E

E

A

A

M

L

S

D

E

P

D

A

F

F

K

K

V

E

Y

Y

Q

S

H

E

Q

Q

I

I

V

I

K

A

N

N

A

A

K

K

R

A

L

M

S

V

E

K

R

V

L

F

M

N

K

Q

H

A

-

I

G

G

F

T

K

R

L

V

V

I

S

S

G

G

G

A

T

T

D

D

N

N

H

H

L

L

M

M

L

L

I

I

N

D

L

V

S

R

N

E

S

L

E

G

I

I

T

N

G

G

K

K

E

E

A

A

E

E

E

S

A

I

L

L

G

D

R

S

A

V

N

N

I

I

T

T

V

V

N

N

K
|
K

N

N

T
x
S

V

I

P

P

F

F

E

E

T

T

R

L

S

S

P
|
P

F
|
F

V

K

T

T

S

S

G

G

V

I

R
|
R

I

I

G

G

T

T

P

P

A

A

V

I

T

T

R

R

G

G

M

F

K

K

E

E

S

E

E

D

M

A

D

V

F

K

I

V

G

A

D

E

L

L

I

V

A

V

Q

K

V

A

L

L

S

Q

D

A

I

K

K

D

S

D

D

N

L

A

N

Q

I

L

N

D

D

E

V

V

K

K

S

T

K

G

V

V

L

R

E

E

L

L

C

T

K

E

Q

K

F

F

P

P

L

L

binding (4R)-6-azanyl-4-[3-(hydroxymethyl)-5-phenyl-phenyl]-3-methyl-4-propan-2-yl-1H-pyrano[2,3-c]pyrazole-5-carbonitrile: E51 (= E56), Y58 (= Y63), L115 (= L120), G119 (= G124), H120 (= H125), L121 (= L126), K340 (= K343), S342 (≠ T345), P350 (= P353), F351 (= F354), R357 (= R360)
binding pyridoxal-5'-phosphate: Y49 (= Y54), S91 (= S96), G92 (= G97), S93 (= S98), H120 (= H125), S170 (= S175), D195 (= D200), A197 (= A202), K224 (= K229), G255 (= G259)

6ymfA Crystal structure of serine hydroxymethyltransferase from aphanothece halophytica in the plp-serine external aldimine state (see paper)
59% identity, 97% coverage: 9:413/418 of query aligns to 9:414/418 of 6ymfA

query
sites
6ymfA

Q

Q

A

T

D

D

P

P

E

T

I

I

Y

S

D

G

A

M

L

M

K

Q

K

K

E

E

I

L

E

Q

R

R

Q

Q

E

R

T

E

H

H

I

L

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

F

F

V

T

S

S

P

P

A

A

V

V

L

M

E

A

A

T

Q

Q

G

G

S

S

I

V

M

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

L

P

P

A

K

K

K

R

R

Y

Y

G

G

C

C

E

E

F

F

V

I

D

D

I

E

A

I

E

E

E

Q

L

V

A

A

I

I

K

D

R

R

A

A

K

K

E

E

L

L

F

F

G

G

A

A

E

A

H

S

A

A

N

N

V

V

Q

Q

A

P

H

H

S
|
S

G
|
G

S
x
A

Q

Q

A

A

N

N

M

F

A

A

V

V

Y

F

F

L

A

T

V

L

L

L

K

K

P

P

G

G

D

D

T

K

I

I

M

M

G

G

M

M

N

D

L

L

S

S

H

H

G

G

H
|
H

L

L

T

T

H

H

G

G

S

S

P

P

V

A

N

N

F

V

S

S

G

G

K

K

L

W

F

F

N

E

V

A

I

V

S

H

Y

Y

G

G

V

V

N

S

K

Q

E

E

T

T

E

E

T

Q

I

L

D

D

Y

Y

D

D

E

H

A

I

E

L

K

E

L

V

A

A

T

R

E

Q

H

E

K

R

P

P

K

K

L

L

I

I

M

I

V

C

G

G

A

Y

S
|
S

A

A

Y

Y

P

P

R

R

T

I

I

I

D

N

F

F

K

E

K

K

F

F

R

R

E

A

I

I

A

A

D

D

K

E

V

V

G

G

A

A

V

Y

L

L

V

L

V

A

D
|
D

M

I

A
|
A

H
|
H

I

I

A

A

G

G

L

L

V

V

A

A

A

S

G

G

A

H

H

H

P

P

S

N

P

P

V

V

P

P

Y

H

A

C

D

D

F

V

V

V

T

T

T
|
T

T

T

H

H

K
|
K

T

T

L

L

R

R

G

G

P

P

R
|
R

G

G

L

L

I

I

L

L

C

T

K

R

E

D

-

P

E

E

Y

L

A

G

K

K

T

K

L

F

N

N

S

K

Q

S

I

V

F

F

P

P

G

G

I

T

Q

Q

G

G

P

P

L

L

M

E

H

H

V

V

I

I

A

A

A

G

K

K

A

A

V

V

A

A

L

F

K

G

E

E

A

A

M

L

S

K

E

P

D

E

F

F

K

K

V

A

Y

Y

Q

S

H

G

Q

Q

I

V

V

I

K

A

N

N

A

A

K

Q

R

A

L

M

S

A

E

Q

R

Q

L

L

M

Q

K

N

H

R

G

G

F

F

K

K

L

I

V

V

S

S

G

G

T

T

D

D

N

N

H

H

L

L

M

M

L

L

I

V

N

D

L

L

S

R

N

S

S

V

E

N

I

L

T

T

G

G

K

K

E

Q

A

A

E

D

E

Q

A

L

L

V

G

S

R

D

A

V

N

N

I

I

T

T

V

A

N

N

K

K

N

N

T

T

V

V

P

P

F

F

E

D

T

P

R

E

S

S

P

P

F

F

V

V

T

T

S

S

G

G

V

L

R
|
R

I

L

G

G

T

S

P

P

A

A

V

M

T

T

R

R

G

G

M

L

K

G

E

T

S

E

E

D

M

F

D

A

F

E

I

I

G

A

D

N

L

I

I

I

A

A

Q

D

V

R

L

L

S

Q

D

N

I

P

K

E

S

D

D

E

L

Q

N

V

I

K

N

Q

D

A

V

C

K

V

S

Q

K

R

V

V

L

A

E

A

L

L

C

C

K

E

Q

R

F

F

P

P

L

L

Y

Y

active site: Y54 (= Y54), E56 (= E56), D200 (= D200), T226 (= T226), K229 (= K229), R235 (= R235)
binding [3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-serine: S34 (= S34), S96 (= S96), G97 (= G97), A98 (≠ S98), H125 (= H125), S175 (= S175), D200 (= D200), A202 (= A202), H203 (= H203), T226 (= T226), K229 (= K229), R361 (= R360)

6ymdA Crystal structure of serine hydroxymethyltransferase from aphanothece halophytica in the covalent complex with malonate (see paper)
59% identity, 97% coverage: 9:413/418 of query aligns to 9:414/420 of 6ymdA

query
sites
6ymdA

Q

Q

A

T

D

D

P

P

E

T

I

I

Y

S

D

G

A

M

L

M

K

Q

K

K

E

E

I

L

E

Q

R

R

Q

Q

E

R

T

E

H

H

I

L

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

F

F

V

T

S

S

P

P

A

A

V

V

L

M

E

A

A

T

Q

Q

G

G

S

S

I

V

M

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

L

P

P

A

K

K

K

R

R

Y

Y

Y
|
Y

G

G

C

C

E

E

F

F

V

I

D

D

I

E

A

I

E

E

E

Q

L

V

A

A

I

I

K

D

R

R

A

A

K

K

E

E

L

L

F

F

G

G

A

A

E

A

H

S

A

A

N

N

V

V

Q

Q

A

P

H

H

S
|
S

G
|
G

S
x
A

Q

Q

A

A

N

N

M

F

A

A

V

V

Y

F

F

L

A

T

V

L

L

L

K

K

P

P

G

G

D

D

T

K

I

I

M

M

G

G

M

M

N

D

L

L

S

S

H

H

G

G

H
|
H

L

L

T

T

H

H

G

G

S

S

P

P

V

A

N

N

F

V

S

S

G

G

K

K

L

W

F

F

N

E

V

A

I

V

S

H

Y

Y

G

G

V

V

N

S

K

Q

E

E

T

T

E

E

T

Q

I

L

D

D

Y

Y

D

D

E

H

A

I

E

L

K

E

L

V

A

A

T

R

E

Q

H

E

K

R

P

P

K

K

L

L

I

I

M

I

V

C

G

G

A
x
Y

S
|
S

A

A

Y

Y

P

P

R

R

T

I

I

I

D

N

F

F

K

E

K

K

F

F

R

R

E

A

I

I

A

A

D

D

K

E

V

V

G

G

A

A

V

Y

L

L

V

L

V

A

D
|
D

M

I

A
|
A

H
|
H

I

I

A

A

G

G

L

L

V

V

A

A

A

S

G

G

A

H

H

H

P

P

S

N

P

P

V

V

P

P

Y

H

A

C

D

D

F

V

V

V

T

T

T
|
T

T

T

H

H

K
|
K

T

T

L

L

R

R

G

G

P

P

R
|
R

G

G

L

L

I

I

L

L

C

T

K

R

E

D

-

P

E

E

Y

L

A

G

K

K

T

K

L

F

N

N

S

K

Q

S

I

V

F

F

P

P

G

G

I

T

Q

Q

G

G

G
|
G

P

P

L

L

M

E

H

H

V

V

I

I

A

A

A

G

K

K

A

A

V

V

A

A

L

F

K

G

E

E

A

A

M

L

S

K

E

P

D

E

F

F

K

K

V

A

Y

Y

Q

S

H

G

Q

Q

I

V

V

I

K

A

N

N

A

A

K

Q

R

A

L

M

S

A

E

Q

R

Q

L

L

M

Q

K

N

H

R

G

G

F

F

K

K

L

I

V

V

S

S

G

G

T

T

D

D

N

N

H

H

L

L

M

M

L

L

I

V

N

D

L

L

S

R

N

S

S

V

E

N

I

L

T

T

G

G

K

K

E

Q

A

A

E

D

E

Q

A

L

L

V

G

S

R

D

A

V

N

N

I

I

T

T

V

A

N

N

K

K

N

N

T

T

V

V

P

P

F

F

E

D

T

P

R

E

S

S

P

P

F

F

V

V

T

T

S

S

G

G

V

L

R
|
R

I

L

G

G

T

S

P

P

A

A

V

M

T

T

R

R

G

G

M

L

K

G

E

T

S

E

E

D

M

F

D

A

F

E

I

I

G

A

D

N

L

I

I

I

A

A

Q

D

V

R

L

L

S

Q

D

N

I

P

K

E

S

D

D

E

L

Q

N

V

I

K

N

Q

D

A

V

C

K

V

S

Q

K

R

V

V

L

A

E

A

L

L

C

C

K

E

Q

R

F

F

P

P

L

L

Y

Y

active site: Y54 (= Y54), E56 (= E56), D200 (= D200), T226 (= T226), K229 (= K229), R235 (= R235)
binding malonate ion: S34 (= S34), Y54 (= Y54), E56 (= E56), Y64 (= Y64), H125 (= H125), H203 (= H203), K229 (= K229), R361 (= R360)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: Y54 (= Y54), S96 (= S96), G97 (= G97), A98 (≠ S98), H125 (= H125), Y174 (≠ A174), S175 (= S175), D200 (= D200), A202 (= A202), T226 (= T226), K229 (= K229), G261 (= G260)

Q5SI56 Serine hydroxymethyltransferase; SHMT; Serine methylase; EC 2.1.2.1 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
61% identity, 94% coverage: 11:404/418 of query aligns to 8:402/407 of Q5SI56

query
sites
Q5SI56

D

D

P

E

E

A

I

L

Y

F

D

E

A

L

L

I

K

A

K

L

E

E

I

E

E

K

R

R

Q

Q

E

R

T

E

H

G

I

L

E

E

L

L

I

I

A

A

S

S

E

E

N

N

F

F

V

V

S

S

P

K

A

Q

V

V

L

R

E

E

A

A

Q

V

G

G

S

S

I

V

M

L

T

T

N

N

K

K

Y
|
Y

A

A

E

E

G

G

Y

Y

P

P

A

G

K

A

R

R

Y

Y

G

G

C

C

E

E

F

V

V

I

D

D

I

R

A

V

E

E

E

S

L

L

A

A

I

I

K

E

R

R

A

A

K

K

E

A

L

L

F

F

G

G

A

A

E

A

H

W

A

A

N

N

V

V

Q

Q

A

P

H

H

S

S

G
|
G

S
|
S

Q

Q

A

A

N

N

M

M

A

A

V

V

Y

Y

F

M

A

A

V

L

L

M

K

E

P

P

G

G

D

D

T

T

I

L

M

M

G

G

M

M

N

D

L

L

S

A

H

A

G

G

H

H

L

L

T

T

H

H

G

G

S

S

P

R

V

V

N

N

F

F

S

S

G

G

K

K

L

L

F

Y

N

K

V

V

I

V

S

S

Y

Y

G

G

V

V

N

R

K

P

E

D

T

T

E

E

T

L

I

I

D

D

Y

L

D

E

E

E

A

V

E

R

K

R

L

L

A

A

T

L

E

E

H

H

K

R

P

P

K

K

L

V

I

I

M

V

V

A

G

G

A

A

S
|
S

A

A

Y

Y

P

P

R

R

T

F

I

W

D

D

F

F

K

K

K

A

F

F

R

R

E

E

I

I

A

A

D

D

K

E

V

V

G

G

A

A

V

Y

L

L

V

V

D

D

M

M

A

A

H
|
H

I

F

A

A

G

G

L

L

V

V

A

A

G

G

A

L

H

H

P

P

S

N

P

P

V

L

P

P

Y

Y

A

A

D

H

F

V

V

V

T

T

T

S

T

T

H
|
H

K
|
K

T

T

L

L

R

R

G

G

P

P

R

R

G

G

L

L

I

I

L

L

C

S

K

N

E

D

-

P

E

E

Y

L

A

G

K

K

T

R

L

I

N

D

S

K

Q

L

I

I

F

F

P

P

G

G

I

I

Q

Q

G

G

G
|
G

P

P

L

L

M

E

H

H

V

V

I

I

A

A

A

G

K

K

A

A

V

V

A

A

L

F

K

F

E

E

A

A

M

L

S

Q

E

P

D

E

F

F

K

K

V

E

Y

Y

Q

S

H

R

Q

L

I

V

V

V

K

E

N

N

A

A

K

K

R

R

L

L

S

A

E

E

R

E

L

L

M

A

K

R

H

R

G

G

F

Y

K

R

L

I

V

V

S

T

G

G

T

T

D

D

N

N

H

H

L

L

M

F

L

L

I

V

N

D

L

L

S

R

N

P

S

K

E

G

I

L

T

T

G

G

K

K

E

E

A

A

E

E

A

R

L

L

G

D

R

A

A

V

N

G

I

I

T

T

V

V

N

N

K

K

N

N

T

A

V

I

P

P

F

F

E

D

T

P

R

K

S

P

P

P

F

R

V

V

T

T

S

S

G

G

V

I

R

R

I

I

G

G

T

T

P

P

A

A

V

I

T

T

R

R

G

G

M

F

K

T

E

P

S

E

E

E

M

M

D

P

F

L

I

V

G

A

D

E

L

L

I

I

A

D

Q

R

V

A

L

L

S

L

D

E

I

G

K

P

S

S

D

E

L

A

N

L

I

R

N

E

D

E

V

V

K

R

S

R

K

L

V

A

L

L

Y51 (= Y54) binding pyridoxal 5'-phosphate
GS 94:95 (= GS 97:98) binding pyridoxal 5'-phosphate
S172 (= S175) binding pyridoxal 5'-phosphate
H200 (= H203) binding pyridoxal 5'-phosphate
H225 (= H228) binding pyridoxal 5'-phosphate
K226 (= K229) modified: N6-(pyridoxal phosphate)lysine
G258 (= G260) binding pyridoxal 5'-phosphate

8suiB Joint x-ray/neutron structure of thermus thermophilus serine hydroxymethyltransferase (tthshmt) in internal aldimine state with l- ser bound in a pre-michalis complex (see paper)
61% identity, 94% coverage: 11:404/418 of query aligns to 3:397/402 of 8suiB

query
sites
8suiB

D

D

P

E

E

A

I

L

Y

F

D

E

A

L

L

I

K

A

K

L

E

E

I

E

E

K

R

R

Q

Q

E

R

T

E

H

G

I

L

E

E

L

L

I

I

A

A

S

S

E

E

N

N

F

F

V

V

S

S

P

K

A

Q

V

V

L

R

E

E

A

A

Q

V

G

G

S

S

I

V

M

L

T

T

N

N

K

K

Y

Y

A

A

E

E

G

G

Y

Y

P

P

A

G

K

A

R

R

Y
|
Y

G

G

C

C

E

E

F

V

V

I

D

D

I

R

A

V

E

E

E

S

L

L

A

A

I

I

K

E

R

R

A

A

K

K

E

A

L

L

F

F

G

G

A

A

E

A

H

W

A

A

N

N

V

V

Q

Q

A

P

H

H

S

S

G

G

S

S

Q

Q

A

A

N

N

M

M

A

A

V

V

Y

Y

F

M

A

A

V

L

L

M

K

E

P

P

G

G

D

D

T

T

I

L

M

M

G

G

M

M

N

D

L

L

S

A

H

A

G

G

H

H

L

L

T

T

H

H

G

G

S

S

P

R

V

V

N

N

F

F

S

S

G

G

K

K

L

L

F

Y

N

K

V

V

I

V

S

S

Y

Y

G

G

V

V

N

R

K

P

E

D

T

T

E

E

T

L

I

I

D

D

Y

L

D

E

E

E

A

V

E

R

K

R

L

L

A

A

T

L

E

E

H

H

K

R

P

P

K

K

L

V

I

I

M

V

V

A

G

G

A

A

S

S

A

A

Y

Y

P

P

R

R

T

F

I

W

D

D

F

F

K

K

K

A

F

F

R

R

E

E

I

I

A

A

D

D

K

E

V

V

G

G

A

A

V

Y

L

L

V

V

D

D

M

M

A

A

H

H

I

F

A

A

G

G

L

L

V

V

A

A

G

G

A

L

H

H

P

P

S

N

P

P

V

L

P

P

Y

Y

A

A

D

H

F

V

V

V

T

T

T

S

T

T

H

H

K

K

T

T

L

L

R

R

G

G

P

P

R

R

G

G

L

L

I

I

L

L

C

S

K

N

E

D

-

P

E

E

Y

L

A

G

K

K

T

R

L

I

N

D

S

K

Q

L

I

I

F

F

P

P

G

G

I

I

Q

Q

G

G

P

P

L

L

M

E

H

H

V

V

I

I

A

A

A

G

K

K

A

A

V

V

A

A

L

F

K

F

E

E

A

A

M

L

S

Q

E

P

D

E

F

F

K

K

V

E

Y

Y

Q

S

H

R

Q

L

I

V

V

V

K

E

N

N

A

A

K

K

R

R

L

L

S

A

E

E

R

E

L

L

M

A

K

R

H

R

G

G

F

Y

K

R

L

I

V

V

S

T

G

G

T

T

D

D

N

N

H

H

L

L

M

F

L

L

I

V

N

D

L

L

S

R

N

P

S

K

E

G

I

L

T

T

G

G

K

K

E

E

A

A

E

E

A

R

L

L

G

D

R

A

A

V

N

G

I

I

T

T

V

V

N

N

K

K

N

N

T

A

V

I

P

P

F

F

E

D

T

P

R

K

S

P

P

P

F

R

V

V

T

T

S

S

G

G

V

I

R

R

I

I

G

G

T

T

P

P

A

A

V

I

T

T

R

R

G

G

M

F

K

T

E

P

S

E

E

E

M

M

D

P

F

L

I

V

G

A

D

E

L

L

I

I

A

D

Q

R

V

A

L

L

S

L

D

E

I

G

K

P

S

S

D

E

L

A

N

L

I

R

N

E

D

E

V

V

K

R

S

R

K

L

V

A

L

L

binding serine: Y55 (= Y63), Y56 (= Y64)

Query Sequence

>WP_013450717.1 NCBI__GCF_000183405.1:WP_013450717.1
MNLYNAVRQADPEIYDALKKEIERQETHIELIASENFVSPAVLEAQGSIMTNKYAEGYPA
KRYYGGCEFVDIAEELAIKRAKELFGAEHANVQAHSGSQANMAVYFAVLKPGDTIMGMNL
SHGGHLTHGSPVNFSGKLFNVISYGVNKETETIDYDEAEKLATEHKPKLIMVGASAYPRT
IDFKKFREIADKVGAVLVVDMAHIAGLVAAGAHPSPVPYADFVTTTTHKTLRGPRGGLIL
CKEEYAKTLNSQIFPGIQGGPLMHVIAAKAVALKEAMSEDFKVYQHQIVKNAKRLSERLM
KHGFKLVSGGTDNHLMLINLSNSEITGKEAEEALGRANITVNKNTVPFETRSPFVTSGVR
IGTPAVTTRGMKESEMDFIGDLIAQVLSDIKSDLNINDVKSKVLELCKQFPLYRGKLY

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory