SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013451996.1 NCBI__GCF_000183405.1:WP_013451996.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07821 Iron(3+)-hydroxamate import ATP-binding protein FhuC; Ferric hydroxamate uptake protein C; Ferrichrome transport ATP-binding protein FhuC; Iron(III)-hydroxamate import ATP-binding protein FhuC; EC 7.2.2.16 from Escherichia coli (strain K12) (see 2 papers)
34% identity, 87% coverage: 17:253/273 of query aligns to 12:261/265 of P07821

query
sites
P07821

F

F

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K50 (= K42) mutation to Q: Lack of activity.
D172 (= D164) mutation to E: Lack of activity.
E173 (= E165) mutation to A: Lack of activity.

1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
31% identity, 82% coverage: 4:227/273 of query aligns to 7:216/353 of 1vciA

query
sites
1vciA

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binding adenosine-5'-triphosphate: F16 (≠ Y13), F19 (≠ Q16), S41 (≠ N38), G42 (= G39), G44 (= G41), K45 (= K42), T46 (= T43), T47 (= T44)

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
30% identity, 80% coverage: 12:230/273 of query aligns to 14:231/343 of P30750

query
sites
P30750

G

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40:46 (vs. 38:44, 57% identical) binding ATP
E166 (= E165) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding L-methionine
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding L-methionine

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
29% identity, 80% coverage: 12:230/273 of query aligns to 15:232/344 of 6cvlD

query
sites
6cvlD

G

G

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I

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binding phosphothiophosphoric acid-adenylate ester: I19 (≠ Q16), S41 (≠ N38), G42 (= G39), A43 (≠ S40), G44 (= G41), K45 (= K42), S46 (≠ T43), T47 (= T44), N141 (≠ E139), S143 (= S141), Q146 (= Q144), H200 (= H198)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 12, 14

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
29% identity, 80% coverage: 12:230/273 of query aligns to 15:232/344 of 3tuzC

query
sites
3tuzC

G

G

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H

binding adenosine-5'-diphosphate: G42 (= G39), G44 (= G41), K45 (= K42), S46 (≠ T43), T47 (= T44)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12, 14
binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
29% identity, 80% coverage: 12:230/273 of query aligns to 15:232/344 of 3tuiC

query
sites
3tuiC

G

G

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T

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R

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N

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V

N

D

Q

G

R

Q

D

E

L

L

F

T

S

T

L

L

N

S

N

E

K

S

E

E

R

L

T

T

R

K

K

A

I

R

A

R

Y

Q

V

I

S

G

Q

M

F

I

V

F

E

Q

N

H

K

F

D

N

I

L

K

L

V

S

I

S

D

R

Y

T

V

V

L

F

A

G

G

N

-

V

R

A

L

L

P

P

H

-

F

-

K

-

F

L

Q

E

I

L

F

D

E

N

T

T

Q

P

K

K

D

D

I

-

E

E

L

V

S

K

H

R

K

R

-

V

-

T

-

E

F

L

M

L

N

S

F

L

F

V

G

G

I

L

E

G

R

D

F

K

K

H

D

D

R

S

F

Y

M

P

Y

S

E

N

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

V

R

V

V

A

A

I

I

C

A

S

R

A

A

L

L

V

A

Q

S

E

N

P

P

E

K

L

V

L

L

L

C

D

D

E

Q

P

A

T

T

A

S

H

A

L

L

D

D

L

P

N

A

Y

T

Q

T

I

R

K

S

I

I

L

L

D

E

L

L

Q

K

E

D

L

I

N

N

T

R

Q

R

M

L

N

G

L

L

S

T

I

I

I

L

S

L

I

I

F

T

H

H

D

E

L

M

N

D

L

V

A

V

S

K

E

R

Y

I

C

C

N

D

Y

C

L

V

A

A

L

V

L

I

R

S

D

N

G

G

E

E

L

L

F

I

K

E

F

Q

G

D

T

T

P

V

G

S

E

E

V

V

L

F

R

S

Y

H

binding adenosine-5'-diphosphate: G42 (= G39), A43 (≠ S40), G44 (= G41), K45 (= K42), S46 (≠ T43), T47 (= T44)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

8g4cB Bceabs atpgs high res tm (see paper)
30% identity, 82% coverage: 15:239/273 of query aligns to 19:242/248 of 8g4cB

query
sites
8g4cB

E

K

Q

Q

F

E

L

V

L

L

K

K

N

G

I

I

T

D

L

I

R

H

L

I

P

E

K

K

S

G

S

E

F

F

L

V

G

S

I

I

I

M

G

G

P

A

N
x
S

G

G

S
|
S

G

G

K

K

T
|
T

T

T

L

L

K

N

T

V

I

L

A

S

G

S

I

I

L

D

R

Q

P

V

F

S

S

H

G

G

T

T

I

I

Y

H

I

I

N

N

Q

G

R

N

D

D

L

M

F

T

S

A

L

M

N

K

N

E

K

K

E

Q

-

L

-

A

-

E

-

F

R

R

T

K

R

Q

K

H

I

L

A

G

Y

F

V

I

S

F

Q

Q

F

-

V

-

E

-

N

-

K

-

D

D

I

Y

K

N

V

L

I

L

D

D

Y

T

V

L

L

T

A

V

G

K

R

E

-

N

-

I

-

L

L

L

P

P

-

L

H

S

F

I

K

T

K

K

F

L

Q

S

I

K

F

K

E

E

T

A

Q

N

K

R

D

K

I

F

E

E

L

E

S

V

H

A

K

K

F

E

M

L

N

-

F

-

G

G

I

I

E

Y

R

E

F

L

K

R

D

D

R

K

F

Y

M

P

Y

N

E

E

L

I

S

S

G

G

Q

Q

Q

K

Q

Q

V

R

V

T

A

S

I

A

C

G

S

R

A

A

L

F

V

I

Q

H

E

D

P

P

E

S

L

I

L

F

L

A

D
|
D

E

E

P

P

T

T

A

G

H

A

L

L

D

D

L

S

N

K

Y

S

Q

A

I

S

K

D

I

L

L

L

D

N

L

K

L

L

Q

S

E

Q

L

L

N

N

T

Q

Q

K

M

R

N

N

L

A

S

T

I

I

S

M

I

V

F

T

H

H

D

D

L

-

N

P

L

V

A

A

S

A

E

S

Y

Y

C

C

N

G

Y

R

L

V

A

I

L

F

L

I

R

K

D

D

G

G

E

Q

L

M

F

Y

K

T

F

Q

G

L

T

N

P

K

G

G

E

G

V

Q

L

D

R

R

Y

Q

D

T

I

F

L

F

E

Q

E

D

I

I

Y

M

K

K

T

T

binding phosphothiophosphoric acid-adenylate ester: S42 (≠ N38), S44 (= S40), T47 (= T43), D168 (= D164)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 13

1f3oA Crystal structure of mj0796 atp-binding cassette (see paper)
33% identity, 74% coverage: 17:218/273 of query aligns to 19:223/232 of 1f3oA

query
sites
1f3oA

F

Y

L

A

L

L

K

K

N

N

I

V

T

N

L

L

R

N

L

I

P

K

K

E

S

G

S

E

F

F

L

V

G

S

I

I

I

M

G

G

P

P

N

S

G
|
G

S

S

G
|
G

K
|
K

T
x
S

T
|
T

L

M

L

L

K

N

T

I

I

I

A

G

G

C

I

L

L

D

R

K

P

P

F

T

S

E

G

G

T

E

I

V

Y

Y

I

I

N

D

Q

N

R

I

D

K

L

T

F

N

S

D

L

L

N

D

K

D

E

E

R

L

T

T

R

K

-

I

-

R

-

D

K

K

I

I

A

G

Y

F

V

V

-

F

S

Q

Q

Q

F

F

-

N

-

L

-

I

-

P

-

L

-

T

-

A

V

L

E

E

N

N

K

V

D

E

I

L

K

P

V

L

I

I

D

F

Y

K

V

Y

L

R

A

G

G

A

R

M

L

S

P

G

H

E

F

E

K

R

F

K

Q

R

I

A

F

L

E

E

T

C

Q

L

K

K

D

M

I

A

E

E

L

L

S

E

H

-

K

-

F

-

M

-

N

-

F

-

G

-

I

-

E

E

R

R

F

F

K

A

D

N

R

H

F

K

M

P

Y

N

E

Q

L

L

S

S

G

G

Q

Q

V

R

V

V

A

A

I

I

C

A

S

R

A

A

L

L

V

A

Q

N

E

N

P

P

E

P

L

I

L

L

L

A

D

D

E

E

P

P

T

T

A

G

H

A

L

L

D

D

L

S

N

K

Y

T

Q

G

I

E

K

K

I

I

L

M

D

Q

L

L

Q

K

E

K

L

L

N

N

T

E

Q

E

M

D

N

G

L

K

S

T

I

V

S

V

I

V

F

T

H

H

D

D

L

I

N

N

L

V

A

A

S

R

E

-

Y

F

C

G

N

E

Y

R

L

I

A

I

L

Y

L

L

R

K

D

D

G

G

E

E

L

V

binding adenosine-5'-diphosphate: G41 (= G39), G43 (= G41), K44 (= K42), S45 (≠ T43), T46 (= T44)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 11

7tchB Bceab e169q variant atp-bound conformation (see paper)
30% identity, 82% coverage: 15:239/273 of query aligns to 18:241/245 of 7tchB

query
sites
7tchB

E

K

Q
|
Q

F

E

L
x
V

L

L

K

K

N

G

I

I

T

D

L

I

R

H

L

I

P

E

K

K

S

G

S

E

F

F

L

V

G

S

I

I

I

M

G

G

P

A

N
x
S

G
|
G

S
|
S

G

G

K
|
K

T
|
T

T

T

L

L

K

N

T

V

I

L

A

S

G

S

I

I

L

D

R

Q

P

V

F

S

S

H

G

G

T

T

I

I

Y

H

I

I

N

N

Q

G

R

N

D

D

L

M

F

T

S

A

L

M

N

K

N

E

K

K

E

Q

-

L

-

A

-

E

-

F

R

R

T

K

R

Q

K

H

I

L

A

G

Y

F

V

I

S

F

Q

Q

F

-

V

-

E

-

N

-

K

-

D

D

I

Y

K

N

V

L

I

L

D

D

Y

T

V

L

L

T

A

V

G

K

R

E

-

N

-

I

-

L

L

L

P

P

-

L

H

S

F

I

K

T

K

K

F

L

Q

S

I

K

F

K

E

E

T

A

Q

N

K

R

D

K

I

F

E

E

L

E

S

V

H

A

K

K

F

E

M

L

N

-

F

-

G

G

I

I

E

Y

R

E

F

L

K

R

D

D

R

K

F

Y

M

P

Y

N

E
|
E

L

I

S

S

G
|
G

Q

Q

Q

K

Q

Q

V

R

V

T

A

S

I

A

C

G

S

R

A

A

L

F

V

I

Q

H

E

D

P

P

E

S

L

I

L

F

L

A

D

D

E

Q

P

P

T

T

A

G

H

A

L

L

D

D

L

S

N

K

Y

S

Q

A

I

S

K

D

I

L

L

L

D

N

L

K

L

L

Q

S

E

Q

L

L

N

N

T

Q

Q

K

M

R

N

N

L

A

S

T

I

I

S

M

I

V

F

T

H

H

D

D

L

-

N

P

L

V

A

A

S

A

E

S

Y

Y

C

C

N

G

Y

R

L

V

A

I

L

F

L

I

R

K

D

D

G

G

E

Q

L

M

F

Y

K

T

F

Q

G

L

T

N

P

K

G

G

E

G

V

Q

L

D

R

R

Y

Q

D

T

I

F

L

F

E

Q

E

D

I

I

Y

M

K

K

T

T

binding adenosine-5'-triphosphate: Q19 (= Q16), V21 (≠ L18), S41 (≠ N38), G42 (= G39), S43 (= S40), K45 (= K42), T46 (= T43), E142 (= E139), G145 (= G142), G146 (= G143)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12

1l2tA Dimeric structure of mj0796, a bacterial abc transporter cassette (see paper)
33% identity, 74% coverage: 17:218/273 of query aligns to 19:223/230 of 1l2tA

query
sites
1l2tA

F

Y

L

A

L

L

K

K

N

N

I

V

T

N

L

L

R

N

L

I

P

K

K

E

S

G

S

E

F

F

L

V

G

S

I

I

I

M

G

G

P

P

N
x
S

G
|
G

S
|
S

G
|
G

K
|
K

T
x
S

T
|
T

L

M

L

L

K

N

T

I

I

I

A

G

G

C

I

L

L

D

R

K

P

P

F

T

S

E

G

G

T

E

I

V

Y

Y

I

I

N

D

Q

N

R

I

D

K

L

T

F

N

S

D

L

L

N

D

K

D

E

E

R

L

T

T

R

K

-

I

-

R

-

D

K

K

I

I

A

G

Y

F

V

V

-

F

S

Q

Q

Q

F

F

-

N

-

L

-

I

-

P

-

L

-

T

-

A

V

L

E

E

N

N

K

V

D

E

I

L

K

P

V

L

I

I

D

F

Y

K

V

Y

L

R

A

G

G

A

R

M

L

S

P

G

H

E

F

E

K

R

F

K

Q

R

I

A

F

L

E

E

T

C

Q

L

K

K

D

M

I

A

E

E

L

L

S

E

H

-

K

-

F

-

M

-

N

-

F

-

G

-

I

-

E

E

R

R

F
|
F

K

A

D

N

R

H

F

K

M

P

Y

N

E
x
Q

L

L

S
|
S

G

G

G
|
G

Q
|
Q

Q

Q

V

R

V

V

A

A

I

I

C

A

S

R

A

A

L

L

V

A

Q

N

E

N

P

P

E

P

L

I

L

L

L

A

D

D

E

Q

P

P

T

T

A

G

H

A

L

L

D

D

L

S

N

K

Y

T

Q

G

I

E

K

K

I

I

L

M

D

Q

L

L

Q

K

E

K

L

L

N

N

T

E

Q

E

M

D

N

G

L

K

S

T

I

V

S

V

I

V

F

T

H
|
H

D

D

L

I

N

N

L

V

A

A

S

R

E

-

Y

F

C

G

N

E

Y

R

L

I

A

I

L

Y

L

L

R

K

D

D

G

G

E

E

L

V

binding adenosine-5'-triphosphate: S40 (≠ N38), G41 (= G39), S42 (= S40), G43 (= G41), K44 (= K42), S45 (≠ T43), T46 (= T44), F138 (= F132), Q145 (≠ E139), S147 (= S141), G149 (= G143), Q150 (= Q144), H204 (= H198)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 11

3d31A Modbc from methanosarcina acetivorans (see paper)
32% identity, 82% coverage: 3:227/273 of query aligns to 1:215/348 of 3d31A

query
sites
3d31A

F

M

I

I

T

E

I

I

E

E

N

S

L

L

I

S

S

R

G

K

Y

W

R

K

E

-

Q

N

F

F

L

S

L

L

K

D

N

N

I

L

T

S

L

L

R

K

L

V

P

E

K

S

S

G

S

E

F

Y

L

F

G

V

I

I

I

L

G

G

P

P

N

T

G

G

S

A

G

G

K

K

T

T

T

L

L

F

L

L

K

E

T

L

I

I

A

A

G

G

I

F

L

H

R

V

P

P

F

D

S

S

G

G

T

R

I

I

Y

L

I

L

N

D

Q

G

R

K

D

D

L

V

F

T

S

D

L

L

N

S

N

P

K

E

E

K

R

H

T

D

R

-

K

-

I

I

A

A

Y

F

V

V

S

Y

Q

Q

F

-

V

-

E

-

N

-

K

-

D

-

I

-

K

-

V

-

I

-

D

N

Y

Y

V

S

L

L

A

-

G

-

R

-

L

F

P

P

H

H

F

M

-

N

-

V

-

K

K

K

K

N

F

L

Q

E

I

F

F

G

E

M

T

R

Q

M

K

K

D

K

I

I

E

K

L

D

S

P

H

K

K

R

F

V

M

L

N

D

F

T

-

A

-

R

-

D

F

L

G

K

I

I

E

E

R

H

F

L

K

L

D

D

R

R

F

N

M

P

Y

L

E

T

L

L

S

S

G

G

Q

E

Q

Q

V

R

V

V

A

A

I

L

C

A

S

R

A

A

L

L

V

V

Q

T

E

N

P

P

E

K

L

I

L

L

D

D

E

E

P

P

T

L

A

S

H

A

L

L

D

D

L

P

N

R

Y

T

Q

Q

I

E

K

N

I

A

L

R

D

E

L

M

L

L

Q

S

E

V

L

L

N

H

T

K

Q

K

M

N

N

K

L

L

S

T

I

V

I

L

S

H

I

I

F

T

H

H

D

D

L

Q

N

T

L

E

A

A

S

R

E

I

Y

M

C

A

N

D

Y

R

L

I

A

A

L

V

R

M

D

D

G

G

E

K

L

L

F

I

K

Q

F

V

G

G

T

K

P

P

G

E

E

E

V

I

Sites not aligning to the query:

binding tungstate(vi)ion: 286, 320, 323, 340, 341, 342

5xu1B Structure of a non-canonical abc transporter from streptococcus pneumoniae r6 (see paper)
32% identity, 79% coverage: 2:216/273 of query aligns to 2:218/226 of 5xu1B

query
sites
5xu1B

E

Q

F

L

I

I

T

S

I

L

E

K

N

N

L

I

I

F

S

R

G

S

Y

Y

R

R

-

N

-

G

-

D

-

Q

E

E

Q

L

F

Q

L

V

L

L

K

K

N

N

I

I

T

N

L

L

R

E

L

V

P

N

K

E

S

G

S

E

F

F

L

V

G

A

I

I

I

M

G

G

P

P

N

S

G

G

S

S

G
|
G

K
|
K

T
x
S

T

T

L

L

L

M

K

N

T

T

I

I

A

G

G

M

I

L

L

D

R

T

P

P

F

T

S

S

G

G

T

E

I

Y

Y

Y

I

L

N

E

Q

G

R

Q

D

E

L

V

F

A

S

G

L

L

N

G

N

E

K

K

E

Q

-

L

-

A

-

K

-

V

R

R

T

N

R

Q

K

Q

I

I

A

G

Y

F

V

V

-

F

S

Q

Q

Q

F

F

V

F

E

L

N

L

K

S

D

K

I

L

K

N

V

A

I

L

D

Q

Y

N

V

V

L

-

A

-

G

-

R

E

L

L

P

P

H

L

F

I

K

-

F

Y

Q

A

I

G

F

V

E

S

T

S

Q

S

K

K

D

R

I

R

E

K

L

L

S

A

H

E

K

E

F

Y

M

L

N

D

F

K

F

V

G

E

I

L

E

T

R

E

F

R

K

S

D

H

R

H

F

L

M

P

Y

S

E

E

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

V

R

V

V

A

A

I

I

C

A

S

R

A

A

L

L

V

V

Q

N

E

N

P

P

E

S

L

I

L

L

L

A

D

D

E

E

P

P

T

T

A

G

H

A

L

L

D

D

L

T

N

K

Y

T

Q

G

I

N

K

Q

I

I

L

M

D

Q

L

L

Q

V

E

D

L

L

N

N

T

K

Q

E

M

-

N

G

L

K

S

T

I

I

S

M

I

V

F

T

H

H

D

E

L

P

N

E

L

I

A

A

S

A

E

-

Y

Y

C

A

N

K

Y

R

L

Q

A

I

L

V

L

I

R

R

D

D

G

G

binding magnesium ion: G45 (= G41), K46 (= K42), S47 (≠ T43)

1oxvD Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
30% identity, 85% coverage: 19:250/273 of query aligns to 21:249/353 of 1oxvD

query
sites
1oxvD

L

L

K

D

N

N

I

V

T

N

L

I

R

N

L

I

P

E

K

N

S

G

S

E

F

R

L

F

G

G

I

I

I

L

G

G

P

P

N
x
S

G
|
G

S

A

G
|
G

K
|
K

T
|
T

L

F

L

M

K

R

T

I

I

I

A

A

G

G

I

L

L

D

R

V

P

P

F

S

S

T

G

G

T

E

I

L

Y

Y

I

F

N

D

Q

D

R

R

D

-

L

L

F

V

S

A

L

S

N

N

N

G

K

K

-

L

-

I

-

V

-

P

E

P

R

E

T

D

R

R

K

K

I

I

A

G

Y

M

V

V

S

F

Q
|
Q

F

-

V

-

E

-

N

-

K

-

D

-

I

-

K

-

V

-

I

-

D

T

Y

W

V

A

L

L

A

Y

G

P

R

N

L

L

P

T

H

A

F

F

K

E

-

N

-

I

-

A

-

F

-

P

-

L

-

T

-

N

-

M

K

K

F

M

Q

S

I

K

F

E

E

E

T

I

Q

R

K

K

D

R

I

V

E

E

L

E

S

V

H

A

K

K

F

I

M

L

N

D

F

-

G

-

I

I

E

H

R

H

F

V

K

L

D

N

R

H

F

F

M

P

Y

R

E

E

L

L

S

S

G

G

Q

Q

V

R

V

V

A

A

I

L

C

A

S

R

A

A

L

L

V

V

Q

K

E

D

P

P

E

S

L

L

D

D

E
|
E

P

P

T

F

A

S

H

N

L

L

D

D

L

A

N

R

Y

M

Q

R

I

D

K

S

I

A

L

R

D

A

L

L

L

V

Q

K

E

E

L

V

N

Q

T

S

Q

R

M

L

N

G

L

V

S

T

I

L

S

V

I

V

F

S

H

H

D

D

L

P

N

A

L

D

A

I

S

F

E

A

Y

I

C

A

N

D

Y

R

L

V

A

G

L

V

L

L

R

V

D

K

G

G

E

K

L

L

F

V

K

Q

F

V

G

G

T

K

P

P

G

E

E

D

V

L

L

-

R

-

Y

Y

D

D

I

N

L

P

E

V

E

S

I

I

Y

Q

K

V

T

A

V

S

I

L

V

I

T

G

D

E

I

I

N

N

P

E

V

L

S

E

G

G

binding phosphoaminophosphonic acid-adenylate ester: S40 (≠ N38), G41 (= G39), G43 (= G41), K44 (= K42), T45 (= T43), T46 (= T44), Q89 (= Q84), E166 (= E165)
binding magnesium ion: T45 (= T43), Q89 (= Q84)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 13

1oxvA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
30% identity, 85% coverage: 19:250/273 of query aligns to 21:249/353 of 1oxvA

query
sites
1oxvA

L

L

K

D

N

N

I

V

T

N

L

I

R

N

L

I

P

E

K

N

S

G

S

E

F

R

L

F

G

G

I

I

I

L

G

G

P

P

N
x
S

G
|
G

S

A

G
|
G

K
|
K

T
|
T

L

F

L

M

K

R

T

I

I

I

A

A

G

G

I

L

L

D

R

V

P

P

F

S

S

T

G

G

T

E

I

L

Y

Y

I

F

N

D

Q

D

R

R

D

-

L

L

F

V

S

A

L

S

N

N

N

G

K

K

-

L

-

I

-

V

-

P

E

P

R

E

T

D

R

R

K

K

I

I

A

G

Y

M

V

V

S

F

Q

Q

F

-

V

-

E

-

N

-

K

-

D

-

I

-

K

-

V

-

I

-

D

T

Y

W

V

A

L

L

A

Y

G

P

R

N

L

L

P

T

H

A

F

F

K

E

-

N

-

I

-

A

-

F

-

P

-

L

-

T

-

N

-

M

K

K

F

M

Q

S

I

K

F

E

E

E

T

I

Q

R

K

K

D

R

I

V

E

E

L

E

S

V

H

A

K

K

F

I

M

L

N

D

F

-

G

-

I

I

E

H

R

H

F

V

K

L

D

N

R

H

F

F

M

P

Y

R

E

E

L

L

S

S

G

G

Q

Q

V

R

V

V

A

A

I

L

C

A

S

R

A

A

L

L

V

V

Q

K

E

D

P

P

E

S

L

L

D

D

E
|
E

P

P

T

F

A

S

H

N

L

L

D

D

L

A

N

R

Y

M

Q

R

I

D

K

S

I

A

L

R

D

A

L

L

L

V

Q

K

E

E

L

V

N

Q

T

S

Q

R

M

L

N

G

L

V

S

T

I

L

S

V

I

V

F

S

H

H

D

D

L

P

N

A

L

D

A

I

S

F

E

A

Y

I

C

A

N

D

Y

R

L

V

A

G

L

V

L

L

R

V

D

K

G

G

E

K

L

L

F

V

K

Q

F

V

G

G

T

K

P

P

G

E

E

D

V

L

L

-

R

-

Y

Y

D

D

I

N

L

P

E

V

E

S

I

I

Y

Q

K

V

T

A

V

S

I

L

V

I

T

G

D

E

I

I

N

N

P

E

V

L

S

E

G

G

binding phosphoaminophosphonic acid-adenylate ester: S40 (≠ N38), G41 (= G39), G43 (= G41), K44 (= K42), T45 (= T43), T46 (= T44), E166 (= E165)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 13

1oxuA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
30% identity, 85% coverage: 19:250/273 of query aligns to 21:249/353 of 1oxuA

query
sites
1oxuA

L

L

K

D

N

N

I

V

T

N

L

I

R

N

L

I

P

E

K

N

S

G

S

E

F

R

L

F

G

G

I

I

I

L

G

G

P

P

N

S

G
|
G

S

A

G
|
G

K
|
K

T
|
T

L

F

L

M

K

R

T

I

I

I

A

A

G

G

I

L

L

D

R

V

P

P

F

S

S

T

G

G

T

E

I

L

Y

Y

I

F

N

D

Q

D

R

R

D

-

L

L

F

V

S

A

L

S

N

N

N

G

K

K

-

L

-

I

-

V

-

P

E

P

R

E

T

D

R

R

K

K

I

I

A

G

Y

M

V

V

S

F

Q

Q

F

-

V

-

E

-

N

-

K

-

D

-

I

-

K

-

V

-

I

-

D

T

Y

W

V

A

L

L

A

Y

G

P

R

N

L

L

P

T

H

A

F

F

K

E

-

N

-

I

-

A

-

F

-

P

-

L

-

T

-

N

-

M

K

K

F

M

Q

S

I

K

F

E

E

E

T

I

Q

R

K

K

D

R

I

V

E

E

L

E

S

V

H

A

K

K

F

I

M

L

N

D

F

-

G

-

I

I

E

H

R

H

F

V

K

L

D

N

R

H

F

F

M

P

Y

R

E

E

L

L

S

S

G

G

Q

Q

V

R

V

V

A

A

I

L

C

A

S

R

A

A

L

L

V

V

Q

K

E

D

P

P

E

S

L

L

D

D

E

E

P

P

T

F

A

S

H

N

L

L

D

D

L

A

N

R

Y

M

Q

R

I

D

K

S

I

A

L

R

D

A

L

L

L

V

Q

K

E

E

L

V

N

Q

T

S

Q

R

M

L

N

G

L

V

S

T

I

L

S

V

I

V

F

S

H

H

D

D

L

P

N

A

L

D

A

I

S

F

E

A

Y

I

C

A

N

D

Y

R

L

V

A

G

L

V

L

L

R

V

D

K

G

G

E

K

L

L

F

V

K

Q

F

V

G

G

T

K

P

P

G

E

E

D

V

L

L

-

R

-

Y

Y

D

D

I

N

L

P

E

V

E

S

I

I

Y

Q

K

V

T

A

V

S

I

L

V

I

T

G

D

E

I

I

N

N

P

E

V

L

S

E

G

G

binding adenosine-5'-diphosphate: G41 (= G39), G43 (= G41), K44 (= K42), T45 (= T43), T46 (= T44)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 13, 20

Q97UY8 Glucose import ATP-binding protein GlcV; EC 7.5.2.- from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
30% identity, 85% coverage: 19:250/273 of query aligns to 21:249/353 of Q97UY8

query
sites
Q97UY8

L

L

K

D

N

N

I

V

T

N

L

I

R

N

L

I

P

E

K

N

S

G

S

E

F

R

L

F

G

G

I

I

I

L

G

G

P

P

N

S

G

G

S

A

G

G

K

K

T

T

L

F

L

M

K

R

T

I

I

I

A

A

G

G

I

L

L

D

R

V

P

P

F

S

S

T

G

G

T

E

I

L

Y

Y

I

F

N

D

Q

D

R

R

D

-

L

L

F

V

S

A

L

S

N

N

N

G

K

K

-

L

-

I

-

V

-

P

E

P

R

E

T

D

R

R

K

K

I

I

A

G

Y

M

V

V

S

F

Q

Q

F

-

V

-

E

-

N

-

K

-

D

-

I

-

K

-

V

-

I

-

D

T

Y

W

V

A

L

L

A

Y

G

P

R

N

L

L

P

T

H

A

F

F

K

E

-

N

-

I

-

A

-

F

-

P

-

L

-

T

-

N

-

M

K

K

F

M

Q

S

I

K

F

E

E

E

T

I

Q

R

K

K

D

R

I

V

E

E

L

E

S

V

H

A

K

K

F

I

M

L

N

D

F

-

G

-

I

I

E

H

R

H

F

V

K

L

D

N

R

H

F

F

M

P

Y

R

E

E

L

L

S
|
S

G

G

G
|
G

Q

Q

V

R

V

V

A

A

I

L

C

A

S

R

A

A

L

L

V

V

Q

K

E

D

P

P

E

S

L

L

D

D

E
|
E

P

P

T

F

A

S

H

N

L

L

D

D

L

A

N

R

Y

M

Q

R

I

D

K

S

I

A

L

R

D

A

L

L

L

V

Q

K

E

E

L

V

N

Q

T

S

Q

R

M

L

N

G

L

V

S

T

I

L

S

V

I

V

F

S

H

H

D

D

L

P

N

A

L

D

A

I

S

F

E

A

Y

I

C

A

N

D

Y

R

L

V

A

G

L

V

L

L

R

V

D

K

G

G

E

K

L

L

F

V

K

Q

F

V

G

G

T

K

P

P

G

E

E

D

V

L

L

-

R

-

Y

Y

D

D

I

N

L

P

E

V

E

S

I

I

Y

Q

K

V

T

A

V

S

I

L

V

I

T

G

D

E

I

I

N

N

P

E

V

L

S

E

G

G

S142 (= S141) mutation to A: Decrease in ATPase activity. Can form dimers.
G144 (= G143) mutation to A: Loss of ATPase activity. Cannot form dimers. Forms an active heterodimer; when associated with A-166.
E166 (= E165) mutation to A: Loss of ATPase activity. Can form dimers in the presence of ATP-Mg(2+). Forms an active heterodimer; when associated with A-144.; mutation to Q: Strong decrease in ATPase activity. Can form dimers in the presence of ATP alone, without Mg(2+).

2d62A Crystal structure of multiple sugar binding transport atp-binding protein
29% identity, 83% coverage: 1:227/273 of query aligns to 4:230/375 of 2d62A

query
sites
2d62A

M

M

E

A

F

E

I

V

T

K

I

L

E

I

N

N

L

I

I

W

S

K

G

R

Y

F

R

G

E

D

Q

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F

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L

A

L

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K

K

N

D

I

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T

S

L

L

R

E

L

I

P

K

K

D

S

G

S

E

F

F

L

L

G

V

I

L

G

G

P

P

N

S

G
|
G

S
x
C

G
|
G

K
|
K

T
|
T

L

T

L

L

K

R

T

M

I

I

A

A

G

G

I

L

L

E

R

E

P

P

F

T

S

R

G

G

T

Q

I

I

Y

Y

I

I

N

-

Q

E

R

D

D

N

L

L

F

V

S

A

L

D

N

P

N

E

K

K

-

G

-

V

-

F

-

V

-

P

E

P

R

K

T

E

R

R

K

D

I

V

A

A

Y

M

V

V

S

F

Q

Q

F

-

V

-

E

-

N

-

K

-

D

-

I

-

K

-

V

-

I

-

D

S

Y

Y

V

A

L

L

A

-

G

-

R

-

L

Y

P

P

H

H

F

M

K

T

-

V

-

Y

-

D

-

N

-

I

-

A

-

F

-

P

-

L

K

K

F

L

Q

R

I

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F

V

E

P

T

K

Q

Q

K

E

D

I

I

D

E

K

L

R

S

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H

R

K

E

F

V

M

A

N

E

F

M

F

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G

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F

L

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L

D

N

R

R

F

K

M

P

Y

R

E

E

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

V

R

V

V

A

A

I

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C

G

S

R

A

A

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E

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E

K

L

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L

F

L

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L

M

D

D

E

E

P

P

T

L

A

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A

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N

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Q

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M

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L

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T

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Y

I

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T

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H

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D

L

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N

V

L

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A

A

S

M

E

T

Y

M

C

G

N

D

Y

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A

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D

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V

binding pyrophosphate 2-: G42 (= G39), C43 (≠ S40), G44 (= G41), K45 (= K42), T46 (= T43), T47 (= T44)

7ahhC Opua inhibited inward-facing, sbd docked (see paper)
30% identity, 85% coverage: 34:266/273 of query aligns to 57:299/382 of 7ahhC

query
sites
7ahhC

I

I

I

M

G

G

P

L

N

S

G
|
G

S

S

G
|
G

K
|
K

T

S

T

T

L

L

K

R

T

L

I

L

A

N

G

R

I

L

L

I

R

E

P

P

F

T

S

S

G

G

T

K

I

I

Y

F

I

I

N

D

Q

N

R

Q

D

D

L

V

F

A

S

T

L

L

N

N

N

K

K

E

E

D

-

L

-

Q

-

V

R

R

T

R

R

K

K

T

I

M

A

S

Y

M

V

V

S

F

Q

Q

-

N

-

F

-

G

-

L

F

F

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P

E

H

N

R

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T

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I

I

L

K

E

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N

I

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Y

Y

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L

L

A

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G

-

R

-

L

-

P

-

H

-

F

-

K

-

K

E

F

V

Q

Q

I

N

F

V

E

P

T

K

Q

E

K

E

D

R

I

R

E

K

L

R

S

A

H

E

K

K

F

A

M

L

N

D

F

N

F

A

G

N

I

L

E

L

R

D

F

F

K

K

D

D

R

Q

F

Y

M

P

Y

K

E

Q

L

L

S

S

G

G

Q

M

Q

Q

V

R

V

V

A

G

I

L

C

A

S

R

A

A

L

L

V

A

Q

N

E

D

P

P

E

E

L

I

L

L

L

M

D
|
D

E
|
E

P

A

T

F

A

S

H

A

L

L

D

D

L

P

N

L

Y

I

Q

R

I

R

K

E

I

M

L

Q

D

D

L

E

L

L

Q

L

E

E

L

L

N

Q

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A

Q

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M

F

N

Q

L

K

S

T

I

I

S

F

I

V

F

S

H

H

D

D

L

L

N

N

L

E

A

A

S

L

E

R

Y

I

C

G

N

D

Y

R

L

I

A

A

L

I

L

M

R

K

D

D

G

G

E

K

L

I

F

M

K

Q

F

I

G

G

T

T

P

G

G

E

E

E

V

I

L

L

R

T

-

N

-

P

-

A

-

N

-

D

-

Y

-

V

-

K

-

T

-

F

-

V

Y

E

D

D

I

T

L

A

E

E

E

N

I

I

Y

M

K

I

T

P

V

A

I
x
L

V

T

T

T

D

N

I

I

N

D

P

-

V

V

S

D

G

G

N

P

P

S

I

V

V

A

L

L

P

K

A

K

S

M

K

K

M

T

S

E

L

E

K

V

S
|
S

L

L

binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: L275 (≠ I241), S297 (= S264), S298 (= S265)
binding phosphoaminophosphonic acid-adenylate ester: G62 (= G39), G64 (= G41), K65 (= K42), D187 (= D164), E188 (= E165)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 12, 39, 40, 41

7aheC Opua inhibited inward facing (see paper)
30% identity, 85% coverage: 34:266/273 of query aligns to 57:299/382 of 7aheC

query
sites
7aheC

I

I

I

M

G

G

P

L

N

S

G

G

S

S

G

G

K

K

T

S

T

T

L

L

K

R

T

L

I

L

A

N

G

R

I

L

L

I

R

E

P

P

F

T

S

S

G

G

T

K

I

I

Y

F

I

I

N

D

Q

N

R

Q

D

D

L

V

F

A

S

T

L

L

N

N

N

K

K

E

E

D

-

L

-

Q

-

V

R

R

T

R

R

K

K

T

I

M

A

S

Y

M

V

V

S

F

Q

Q

-

N

-

F

-

G

-

L

F

F

V

P

E

H

N

R

K

T

D

I

I

L

K

E

V

N

I

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D

E

Y

Y

V

G

L

L

A

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G

-

R

-

L

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P

-

H

-

F

-

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-

K

E

F

V

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Q

I

N

F

V

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P

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K

Q

E

K

E

D

R

I

R

E

K

L

R

S

A

H

E

K

K

F

A

M

L

N

D

F

N

F

A

G

N

I

L

E

L

R

D

F

F

K

K

D

D

R

Q

F

Y

M

P

Y

K

E

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G

G

Q

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Q

Q

V

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V

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A

G

I

L

C

A

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A

A

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L

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A

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N

E

D

P

P

E

E

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I

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L

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M

D

D

E

E

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A

T

F

A

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H

A

L

L

D

D

L

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N

L

Y

I

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I

R

K

E

I

M

L

Q

D

D

L

E

L

L

Q

L

E

E

L

L

N

Q

T

A

Q

K

M

F

N

Q

L

K

S

T

I

I

S

F

I

V

F

S

H

H

D

D

L

L

N

N

L

E

A

A

S

L

E

R

Y

I

C

G

N

D

Y

R

L

I

A

A

L

I

L

M

R

K

D

D

G

G

E

K

L

I

F

M

K

Q

F

I

G

G

T

T

P

G

G

E

E

E

V

I

L

L

R

T

-

N

-

P

-

A

-

N

-

D

-

Y

-

V

-

K

-

T

-

F

-

V

Y

E

D

D

I

T

L

A

E

E

E

N

I

I

Y

M

K

I

T

P

V

A

I
x
L

V

T

T

T

D

N

I

I

N

D

P

-

V

V

S

D

G

G

N

P

P

S

I

V

V

A

L

L

P

K

A

K

S

M

K

K

M

T

S

E

L

E

K

V

S
|
S

L

L

binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: L275 (≠ I241), S297 (= S264), S298 (= S265)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
31% identity, 77% coverage: 18:227/273 of query aligns to 16:223/240 of 4ymuJ

query
sites
4ymuJ

L
x
V

L

L

K

K

N

G

I

V

T

T

L

L

R

K

L

V

P

N

K

K

S

G

S

E

F

V

L

V

G

V

I

I

G

G

P

P

N
x
S

G
|
G

S
|
S

G
|
G

K
|
K

T
x
S

T
|
T

L

L

K

R

T

C

I

I

A

N

G

L

I

L

L

E

R

E

P

P

F

T

S

K

G

G

T

E

I

V

Y

F

I

I

N

-

Q

-

R

-

D

D

L

G

F

V

S

K

L

I

N

N

K

G

E

K

R

V

T

N

-

I

-

N

-

K

-

V

-

R

R

Q

K

K

I

V

A

G

Y

M

V

V

S

F

Q

Q

-

H

F

F

V

N

E

L

N

F

K

P

D

H

I

L

K

T

V

A

I

I

D

E

Y

N

V

I

L

T

A

L

G

A

R

P

L

V

P

-

H

K

F

V

K

K

F

M

Q

N

I

K

F

K

E

E

T

A

Q

E

K

-

D

-

I

-

E

E

L

L

S

A

H

V

K

D

F

L

M

L

N

A

F

K

F

V

G

G

I

L

E

L

R

D

F

K

K

K

D

D

R

Q

F

Y

M

P

Y

I

E

K

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

V

R

V

L

A

A

I

I

C

A

S

R

A

A

L

L

V

A

Q

M

E

Q

P

P

E

E

L

V

L

M

L

L

L

F

D

D

E
|
E

P

P

T

T

A

S

H

A

L

L

D

D

L

P

N

E

Y

M

Q

V

I

K

K

E

I

V

L

L

D

N

L

V

L

M

Q

K

E

Q

L

L

N

A

T

N

Q

E

M

-

N

G

L

M

S

T

I

M

I

V

S

V

I

V

F

T

H
|
H

D

E

L

M

N

G

L

F

A

A

S

R

E

E

Y

V

C

G

N

D

Y

R

L

V

A

I

L

F

L

M

R

D

D

D

G

G

E

V

L

I

F

V

K

E

F

E

G

G

T

T

P

P

G

E

E

E

V

I

binding adenosine-5'-triphosphate: V16 (≠ L18), S36 (≠ N38), G37 (= G39), S38 (= S40), G39 (= G41), K40 (= K42), S41 (≠ T43), T42 (= T44), E162 (= E165), H194 (= H198)
binding magnesium ion: S41 (≠ T43), E162 (= E165)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 11

Query Sequence

>WP_013451996.1 NCBI__GCF_000183405.1:WP_013451996.1
MEFITIENLISGYREQFLLKNITLRLPKSSFLGIIGPNGSGKTTLLKTIAGILRPFSGTI
YINQRDLFSLNNKERTRKIAYVSQFVENKDIKVIDYVLAGRLPHFKKFQIFETQKDIELS
HKFMNFFGIERFKDRFMYELSGGQQQVVAICSALVQEPELLLLDEPTAHLDLNYQIKILD
LLQELNTQMNLSIISIFHDLNLASEYCNYLALLRDGELFKFGTPGEVLRYDILEEIYKTV
IVTDINPVSGNPIVLPASKMSLKSSLKDDYNER

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory