SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013452043.1 NCBI__GCF_000183405.1:WP_013452043.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

A8B2U2 Fructose-bisphosphate aldolase; Glfba; glFBPA; Fructose-1,6-bisphosphate aldolase; EC 4.1.2.13 from Giardia intestinalis (strain ATCC 50803 / WB clone C6) (Giardia lamblia) (see 4 papers)
48% identity, 97% coverage: 10:324/324 of query aligns to 3:307/323 of A8B2U2

query
sites
A8B2U2

L

L

V

C

N

T

T

L

R

R

E

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M

F

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K

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K

Y

Y

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G

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A

Y

F

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N

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Y

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A

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A

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-

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A

A

A

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L

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M

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H

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M

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D

G

A

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S

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P

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E

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E

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E

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L

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L

A

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G

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N

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G

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H
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H

G
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G

A

A

Y

Y

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K

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F

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K

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K

-

P

-

G

S

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D

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L

L

R

A

F

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D

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R

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L

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G

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H
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H

G
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G

A

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S

S

S

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P

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Y

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I

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N

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A

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A

A

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C

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K

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N
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N

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D

S
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S

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R

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M

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Y

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R

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Y

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A

L

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G

S

S

A

A

G

G

K

H

A

A

S50 (= S57) binding beta-D-fructose 1,6-bisphosphate
D83 (= D94) active site, Proton donor; mutation to A: Severe loss of catalytic activity.
H84 (= H95) binding Zn(2+)
H178 (= H190) binding beta-D-fructose 1,6-bisphosphate; binding Zn(2+)
G179 (= G191) binding beta-D-fructose 1,6-bisphosphate
K182 (= K194) binding beta-D-fructose 1,6-bisphosphate
H210 (= H226) binding Zn(2+)
G211 (= G227) binding beta-D-fructose 1,6-bisphosphate
S213 (= S229) binding beta-D-fructose 1,6-bisphosphate
N253 (= N269) binding beta-D-fructose 1,6-bisphosphate
D255 (= D271) binding beta-D-fructose 1,6-bisphosphate; mutation to A: 9.4-fold reduction in substrate affinity and 50-fold reduction in catalytic affinity. Has some activity towards tagatose-1,6-bisphosphate.
S256 (= S272) binding beta-D-fructose 1,6-bisphosphate
R259 (= R275) binding beta-D-fructose 1,6-bisphosphate; mutation to A: 1.8-fold reduction in substrate affinity and 2.8-fold reduction in catalytic efficiency. 6-fold reduction in substrate affinity and 24-fold reduction in catalytic efficiency; when associated with A-278.
D278 (= D294) mutation to A: 159-fold reduction in substrate affinity and 2770-fold reduction in catalytic efficiency. 6-fold reduction in substrate affinity and 24-fold reduction in catalytic efficiency; when associated with A-259.
R280 (= R296) binding beta-D-fructose 1,6-bisphosphate

3ohiA Structure of giardia fructose-1,6-biphosphate aldolase in complex with 3-hydroxy-2-pyridone (see paper)
47% identity, 97% coverage: 10:324/324 of query aligns to 2:303/319 of 3ohiA

query
sites
3ohiA

L

L

V

C

N

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T

L

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E

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M

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F

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M

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D

G

A

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S

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H

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E

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L

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L

A

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N

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A

-

E

-

H

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Q

Y

L

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T

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E

P

P

A

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Q

D

V

A

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K

F

F

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T

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G

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V

D

D

S

A

L

L

A

A

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A

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I

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G

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S

H
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H

G
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G

A

A

Y

Y

K
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K

F

F

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K

-

P

-

G

S

E

E

E

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H

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N

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A

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H

A

A

A

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A

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A

G

V

V

C

C

K

K

I

I

N
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N

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D
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D

S
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S

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R

L

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A

A

F

M

T

T

A

G

K

A

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K

Y

V

L

F

A

V

N

E

N

H

P

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K

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F

D

D

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Y

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G

G

S

S

A

A

G

G

K

H

A

A

binding ({3-hydroxy-2-oxo-4-[2-(phosphonooxy)ethyl]pyridin-1(2H)-yl}methyl)phosphonic acid: S49 (= S57), D82 (= D94), H83 (= H95), H174 (= H190), G175 (= G191), K178 (= K194), G207 (= G227), S209 (= S229), N249 (= N269), D251 (= D271), S252 (= S272), R255 (= R275)
binding zinc ion: H83 (= H95), H174 (= H190), H206 (= H226)

3gayA Structure of giardia fructose-1,6-biphosphate aldolase in complex with tagatose-1,6-biphosphate (see paper)
47% identity, 97% coverage: 10:324/324 of query aligns to 2:303/319 of 3gayA

query
sites
3gayA

L

L

V

C

N

T

T

L

R

R

E

Q

M

M

F

L

K

G

K

E

A

A

M

R

E

K

G

H

K

K

Y

Y

A

G

V

V

P

G

A

A

Y

F

N
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N

F

V

N

N

L

M

E

E

Q

Q

L

I

Q

Q

A

G

I

I

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M

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K

A

A

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S

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N

D

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C

L

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A

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A

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H

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D
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D

H
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H

G

G

D

D

S

T

F

L

E

E

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K

S

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D

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G

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M

M

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I

D

D

G

A

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S

H

H

L

H

P

P

F

F

E

D

E

E

N

N

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A

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K

E

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E

A

Y

Y

A

A

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A

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E

E

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E

E

L

L

G

G

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L

L

A

G

G

G

I

I

E

E

D

Q

D

N

V

T

S

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A

-

E

-

H

-

S

-

H

Q

Y

L

T

T

D

E

P

P

A

Q

Q

D

V

A

E

K

F

F

V

V

A

E

K

L

T

T

G

G

V

V

D

D

S

A

L

L

A

A

I

V

S

A

I

I

G

G

T

T

S

S

H
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H

G
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G

A

A

Y

Y

K
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K

F

F

K

K

V

-

K

-

P

-

G

S

E

E

E

S

V

D

P

I

P

R

L

L

R

A

F

I

D

D

I

R

L

V

E

K

E

T

V

I

E

S

K

D

R

-

L

L

P

T

G

G

F

I

P

P

I

L

V

V

L

M

H
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H

G
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G

A

S

S
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S

S

S

V

V

V

P

P

K

E

D

Y

V

V

K

E

D

L

M

I

I

N

N

K

K

Y

Y

G

G

K

K

L

M

E

P

G

D

A

A

V

V

G

G

V

V

P

P

E

I

E

E

Q

S

L

I

R

V

R

H

A

A

A

I

A

G

S

E

A

G

V

V

C

C

K

K

I

I

N
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N

I

V

D
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D

S
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S

D

D

G

S

R
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R

L

M

A

A

F

M

T

T

A

G

K

A

V

I

R

R

E

K

Y

V

L

F

A

V

N

E

N

H

P

P

K

E

E

K

F

F

D

D

P

P

R

R

K

D

Y

Y

L

L

K

G

P

P

A

G

R

R

E

D

E

A

L

I

V

T

K

E

L

M

Y

L

K

I

H

P

K

K

N

-

I

I

N

K

V

A

L

F

G

G

S

S

A

A

G

G

K

H

A

A

binding 1,6-di-O-phosphono-D-tagatose: N23 (= N31), S49 (= S57), D82 (= D94), H174 (= H190), G175 (= G191), K178 (= K194), H206 (= H226), G207 (= G227), S209 (= S229), N249 (= N269), D251 (= D271), S252 (= S272), R255 (= R275)
binding zinc ion: H83 (= H95), H174 (= H190), H206 (= H226)

3gb6A Structure of giardia fructose-1,6-biphosphate aldolase d83a mutant in complex with fructose-1,6-bisphosphate (see paper)
47% identity, 97% coverage: 10:324/324 of query aligns to 2:302/318 of 3gb6A

query
sites
3gb6A

L

L

V

C

N

T

T

L

R

R

E

Q

M

M

F

L

K

G

K

E

A

A

M

R

E

K

G

H

K

K

Y

Y

A

G

V

V

P

G

A

A

Y

F

N
|
N

F

V

N

N

L

M

E

E

Q

Q

L

I

Q

Q

A

G

I

I

I

M

Q

K

A

A

C

V

V

V

E

Q

T

L

K

K

S

S

P

P

V

V

I

I

L

L

Q

Q

V

C

S
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S

K

R

G

G

A

A

R

L

E

K

Y

Y

A

S

N

D

A

M

T

I

I

Y

L

L

R

K

Y

K

L

L

A

C

L

-

G

-

A

-

T

-

Q

E

L

A

A

A

E

L

E

E

M

K

G

H

H

P

K

D

I

I

P

P

I

I

A

C

L

I

H

H

L

L

D

A

H

H

G

G

D

D

S

T

F

L

E

E

I

S

C

V

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K

S

M

C

A

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I

D

D

Y

L

G

G

F

F

S

S

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V

M

M

I

I

D

D

G

A

S

S

H

H

L

H

P

P

F

F

E

D

E

E

N

N

I

V

A

R

V

I

T

T

R

K

K

E

V

V

E

A

Y

Y

A

A

H

H

Q

A

F

R

D

G

V

V

T

S

V

V

E

E

G

A

E

E

L

L

G

G

V

T

L

L

A

G

G

G

I

I

E

E

D

N

D

T

V

V

S

-

A

-

E

-

H

-

S

-

H

Q

Y

L

T

T

D

E

P

P

A

Q

Q

D

V

A

E

K

F

F

V

V

A

E

K

L

T

T

G

G

V

V

D

D

S

A

L

L

A

A

I

V

S

A

I

I

G

G

T

T

S

S

H
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H

G
|
G

A

A

Y

Y

K
|
K

F

F

K

K

V

-

K

-

P

-

G

S

E

E

E

S

V

D

P

I

P

R

L

L

R

A

F

I

D

D

I

R

L

V

E

K

E

T

V

I

E

S

K

D

R

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L

L

P

T

G

G

F

I

P

P

I

L

V

V

L

M

H
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H

G
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G

A

S

S
|
S

S

S

V

V

V

P

P

K

E

D

Y

V

V

K

E

D

L

M

I

I

N

N

K

K

Y

Y

G

G

K

K

L

M

E

P

G

D

A

A

V

V

G

G

V

V

P

P

E

I

E

E

Q

S

L

I

R

V

R

H

A

A

A

I

A

G

S

E

A

G

V

V

C

C

K

K

I

I

N
|
N

I

V

D
|
D

S
|
S

D

D

G

S

R
|
R

L

M

A

A

F

M

T

T

A

G

K

A

V

I

R

R

E

K

Y

V

L

F

A

V

N

E

N

H

P

P

K

E

E

K

F

F

D

D

P

P

R

R

K

D

Y

Y

L

L

K

G

P

P

A

G

R

R

E

D

E

A

L

I

V

T

K

E

L

M

Y

L

K

I

H

P

K

K

N

-

I

I

N

K

V

A

L

F

G

G

S

S

A

A

G

G

K

H

A

A

binding 1,6-di-O-phosphono-D-fructose: N23 (= N31), S49 (= S57), H173 (= H190), G174 (= G191), K177 (= K194), H205 (= H226), G206 (= G227), S208 (= S229), N248 (= N269), D250 (= D271), S251 (= S272), R254 (= R275)

2isvB Structure of giardia fructose-1,6-biphosphate aldolase in complex with phosphoglycolohydroxamate (see paper)
45% identity, 97% coverage: 10:324/324 of query aligns to 2:292/307 of 2isvB

query
sites
2isvB

L

L

V

C

N

T

T

L

R

R

E

Q

M

M

F

L

K

G

K

E

A

A

M

R

E

K

G

H

K

K

Y

Y

A

G

V

V

P

G

A

A

Y

F

N

N

F

V

N

N

L

M

E

E

Q

Q

L

I

Q

Q

A

G

I

I

I

M

Q

K

A

A

C

V

V

V

E

Q

T

L

K

K

S

S

P

P

V

V

I

I

L

L

Q

Q

V

C

S

S

K

R

G

G

A

A

R

L

E

K

Y

Y

A

S

N

D

A

M

T

I

I

Y

L

L

R

K

Y

K

L

L

A

C

L

-

G

-

A

-

T

-

Q

E

L

A

A

A

E

L

E

E

M

K

G

H

H

P

K

D

I

I

P

P

I

I

A

C

L

I

H

H

L

L

D
|
D

H
|
H

G

G

D

D

S

T

F

L

E

E

I

S

C

V

K

K

S

M

C

A

V

I

D

D

Y

L

G

G

F

F

S

S

V

V

M

M

I

I

D

D

G

A

S

S

H

H

L

H

P

P

F

F

E

D

E

E

N

N

I

V

A

R

V

I

T

T

R

K

K

E

V

V

E

A

Y

Y

A

A

H

H

Q

A

F

R

D

S

V

V

T

S

V

V

E

E

G

A

E

E

L

L

G

G

V

-

L

-

A

-

G

-

I

-

E

-

D

-

V

-

S

-

A

Q

E

N

H

T

S

V

H

Q

Y

L

T

T

D

E

P

P

A

Q

Q

D

V

A

E

K

F

F

V

V

A

E

K

L

T

T

G

G

V

V

D

D

S

A

L

L

A

A

I

V

S

A

I

I

G

G

T

T

S

S

H
|
H

G
|
G

A

A

Y

Y

K
|
K

F

F

K

I

V

-

K

-

P

-

G

-

E

-

V

-

P

-

P

R

L

L

R

A

F

I

D

D

I

R

L

V

E

K

E

T

V

I

E

S

K

D

R

-

L

L

P

T

G

G

F

I

P

P

I

L

V

V

L

M

H
|
H

G
|
G

A

S

S
|
S

S

S

V

V

V

P

P

K

E

D

Y

V

V

K

E

D

L

M

I

I

N

N

K

K

Y

Y

G

G

K

K

L

M

E

P

G

D

A

A

V

V

G

G

V

V

P

P

E

I

E

E

Q

S

L

I

R

V

R

H

A

A

A

I

A

G

S

E

A

G

V

V

C

C

K

K

I

I

N
|
N

I

V

D
|
D

S
|
S

D

D

G

S

R

R

L

M

A

A

F

M

T

T

A

G

K

A

V

I

R

R

E

K

Y

V

L

F

A

V

N

E

N

H

P

P

K

E

E

K

F

F

D

D

P

P

R

R

K

D

Y

Y

L

L

K

G

P

P

A

G

R

R

E

D

E

A

L

I

V

T

K

E

L

M

Y

L

K

I

H

P

K

K

N

-

I

I

N

K

V

A

L

F

G

G

S

S

A

A

G

G

K

H

A

A

binding phosphoglycolohydroxamic acid: D82 (= D94), H168 (= H190), G169 (= G191), K172 (= K194), H195 (= H226), G196 (= G227), S198 (= S229), N238 (= N269), D240 (= D271), S241 (= S272)
binding zinc ion: H83 (= H95), H168 (= H190), H195 (= H226)

1rv8B Class ii fructose-1,6-bisphosphate aldolase from thermus aquaticus in complex with cobalt (see paper)
47% identity, 97% coverage: 10:324/324 of query aligns to 2:305/305 of 1rv8B

query
sites
1rv8B

L

L

V

V

N

T

T

G

R

L

E

E

M

I

F

L

K

K

A

A

M

R

E

E

G

E

K

G

Y

Y

A

G

V

V

P

G

A

A

Y

F

N

N

F

V

N

N

L

M

E

E

Q

F

L

L

Q

Q

A

A

I

V

I

L

Q

E

A

A

C

A

V

E

E

E

T

Q

K

R

S

S

P

P

V

V

I

I

L

L

Q

A

V

L

S

S

K

E

G

G

A

A

R

M

E

K

Y

Y

A

G

N

G

A

-

T

-

I

-

L

-

R

R

Y

A

L

L

A

T

L

L

G

M

A

A

T

V

Q

E

L

L

A

A

E

K

E

E

M

A

G

-

H

-

K

R

I

V

P

P

I

V

A

A

L

V

H

H

L

L

D
|
D

H
|
H

G

G

D

S

S

S

F

Y

E

E

I

S

C

V

K

L

S

R

C

A

V

L

D

R

Y

A

G

G

F

F

S

T

S

S

V

V

M

M

I

I

D

D

G

K

S

S

H

H

L

E

P

D

F

F

E

E

E

T

N

N

I

V

A

R

V

E

T

T

R
|
R

K

R

V

V

E

E

Y

A

A

A

H
|
H

Q

A

F

V

D

G

V

V

T

T

V

V

E

E

G

A

E
|
E

L

L

G

G

V

R

L

L

A

A

G

G

I

I

E
|
E

D

E

D

H

V

V

S

A

A

V

E

D

H

E

S

K

H

D

-

A

-

L

Y

L

T

T

D

N

P

P

A

E

Q

E

V

A

E

R

E

I

F

F

V

M

A

E

K

R

T

T

G

G

V

A

D

D

S

Y

L

L

A

A

I

V

S

A

I

I

G

G

T

T

S

S

H
|
H

G

G

A

A

Y

Y

K

K

F

G

K

K

V

G

K

R

P

P

G

F

E

-

V

-

P

-

L

I

R

D

F

H

D

A

I

R

L

L

E

E

E

R

V

I

E

A

K

R

R

L

L

V

P

P

G

A

F

-

P

P

I

L

V

V

L

L

H
|
H

G

G

A

A

S
|
S

S

A

V

V

V

P

P

P

E

E

Y

L

V

V

E

E

L

R

I

F

N

R

K

A

Y

S

G

G

K

E

L

I

E

G

G

E

A

A

V

A

G

G

V

I

P

H

E

P

E

E

Q

D

L

I

R

K

A

A

A

I

A

S

S

L

A

G

V

I

C

A

K

K

I

I

N
|
N

I

T

D
|
D

S
x
T

D

D

G

L

R

R

L

L

A

A

F

F

T

T

A

A

K

L

V

I

R

R

E

E

Y

A

L

L

A

N

N

K

N

N

P

P

K

K

E

E

F

F

D

D

P

P

R

R

K

K

Y

Y

L

L

K

G

P

P

A

A

R

R

E

E

E

A

L

V

V

K

K

E

L

V

Y

V

K

K

H

S

K

R

N

-

I

M

N

E

V

L

L

F

G

G

S

S

A

V

G

G

K

R

A

A

active site: D80 (= D94), H81 (= H95), E140 (= E154), H178 (= H190), H208 (= H226), N251 (= N269)
binding cobalt (ii) ion: H81 (= H95), E132 (= E146), H178 (= H190), H208 (= H226)
binding sulfate ion: R116 (= R130), H123 (= H137), S211 (= S229), D253 (= D271), T254 (≠ S272)

2isvA Structure of giardia fructose-1,6-biphosphate aldolase in complex with phosphoglycolohydroxamate (see paper)
44% identity, 97% coverage: 10:324/324 of query aligns to 2:283/298 of 2isvA

query
sites
2isvA

L

L

V

C

N

T

T

L

R

R

E

Q

M

M

F

L

K

G

K

E

A

A

M

R

E

K

G

H

K

K

Y

Y

A

G

V

V

P

G

A

A

Y

F

N

N

F

V

N

N

L

M

E

E

Q

Q

L

I

Q

Q

A

G

I

I

I

M

Q

K

A

A

C

V

V

V

E

Q

T

L

K

K

S

S

P

P

V

V

I

I

L

L

Q

Q

V

C

S

S

K

R

G

G

A

A

R

L

E

K

Y

Y

A

S

N

D

A

M

T

I

I

Y

L

L

R

K

Y

K

L

L

A

C

L

-

G

-

A

-

T

-

Q

E

L

A

A

A

E

L

E

E

M

K

G

H

H

P

K

D

I

I

P

P

I

I

A

C

L

I

H

H

L

L

D
|
D

H
|
H

G

G

D

D

S

T

F

L

E

E

I

S

C

V

K

K

S

M

C

A

V

I

D

D

Y

L

G

G

F

F

S

S

V

V

M

M

I

I

D

D

G

A

S

S

H

H

L

H

P

P

F

F

E

D

E

E

N

N

I

V

A

R

V

I

T

T

R

K

K

E

V

V

E

A

Y

Y

A

A

H

H

Q

A

F

R

D

S

V

V

T

S

V

V

E

E

G

A

E

E

L

L

G

G

V

L

L

-

A

-

G

-

I

-

E

-

D

-

V

-

S

-

A

-

E

-

H

-

S

-

H

-

Y

-

T

T

D

E

P

P

A

Q

Q

D

V

A

E

K

F

F

V

V

A

E

K

L

T

T

G

G

V

V

D

D

S

A

L

L

A

A

I

V

S

A

I

I

G

G

T

T

S

S

H
|
H

G

G

A

A

Y

R

K

-

F

-

K

-

V

-

K

-

P

-

G

-

E

-

V

-

P

-

L

L

R

A

F

I

D

D

I

R

L

V

E

K

E

T

V

I

E

S

K

D

R

-

L

L

P

T

G

G

F

I

P

P

I

L

V

V

L

M

H
|
H

G
|
G

A

S

S
|
S

S

S

V

V

V

P

P

K

E

D

Y

V

V

K

E

D

L

M

I

I

N

N

K

K

Y

Y

G

G

K

K

L

M

E

P

G

D

A

A

V

V

G

G

V

V

P

P

E

I

E

E

Q

S

L

I

R

V

R

H

A

A

A

I

A

G

S

E

A

G

V

V

C

C

K

K

I

I

N
|
N

I

V

D
|
D

S
|
S

D

D

G

S

R

R

L

M

A

A

F

M

T

T

A

G

K

A

V

I

R

R

E

K

Y

V

L

F

A

V

N

E

N

H

P

P

K

E

E

K

F

F

D

D

P

P

R

R

K

D

Y

Y

L

L

K

G

P

P

A

G

R

R

E

D

E

A

L

I

V

T

K

E

L

M

Y

L

K

I

H

P

K

K

N

-

I

I

N

K

V

A

L

F

G

G

S

S

A

A

G

G

K

H

A

A

binding phosphoglycolohydroxamic acid: D82 (= D94), H163 (= H190), H186 (= H226), G187 (= G227), S189 (= S229), N229 (= N269), D231 (= D271), S232 (= S272)
binding zinc ion: H83 (= H95), H163 (= H190), H186 (= H226)

3q94A The crystal structure of fructose 1,6-bisphosphate aldolase from bacillus anthracis str. 'Ames ancestor'
44% identity, 97% coverage: 10:324/324 of query aligns to 3:285/285 of 3q94A

query
sites
3q94A

L

L

V

V

N

S

T

M

R

K

E

E

M

M

F

L

K

N

K

K

A

A

M

L

E

E

G

G

K

K

Y

Y

A

A

V

V

P

G

A

Q

Y

F

N

N

F

M

N

N

L

L

E

E

Q

W

L

T

Q

Q

A

A

I

I

I

L

Q

A

A

A

C

A

V

E

E

E

T

E

K

K

S

S

P

P

V

V

I

I

L

L

Q

G

V

V

S

S

K

E

G

G

A

A

R

A

E

R

Y

H

A

M

N

-

A

-

T

T

I

G

L

F

R

K

Y

T

L

V

A

V

L

A

G

M

A

V

T

K

Q

A

L

L

A

I

E

E

M

M

G

N

H

I

K

T

I

V

P

P

I

V

A

A

L

I

H

H

L

L

D
|
D

H
|
H

G

G

D

S

S

S

F

F

E

E

I

K

C

C

K

K

S

E

C

A

V

I

D

D

Y

A

G

G

F

F

S

T

S

S

V

V

M

M

I

I

D

D

G

A

S

S

H

H

L

H

P

P

F

F

E
|
E

E

E

N

N

I

V

A

E

V

T

T

T

R

K

K

K

V

V

E

E

Y

Y

A

A

H

H

Q

A

F

R

D

N

V

V

T

S

V

V

E

E

G

A

E

E

L

L

G

G

V

T

L

V

A

G

G

G

I

Q

E
|
E

D

D

V

V

S

I

A

A

E

E

H

G

S

V

H

I

Y

Y

T

A

D

D

P

P

A

A

Q

E

V

C

E

K

E
x
H

F

L

V

V

A

E

K

A

T

T

G

G

V

I

D

D

S

C

L

L

A

A

I

P

S

A

I

L

G

G

T

S

S

V

H
|
H

G

G

A

P

Y

Y

K

K

F

-

K

-

V

-

K

-

P

-

G

G

E

E

E

-

V

-

P

P

P

N

L

L

R

G

F

F

D

A

I

E

L

M

E

E

E

Q

V

V

E

-

K

R

R

D

L

F

P

T

G

G

F

V

P

P

I

L

V

V

L

L

H
|
H

G

G

A

G

S

T

S

-

V

-

P

-

E

-

Y

-

V

-

E

-

L

-

I

-

N

-

K

-

Y

-

G

-

K

-

L

-

E

-

G

-

A

-

V

-

G

G

V

I

P

P

E

T

E

A

Q

D

L

I

R

E

R

K

A

A

A

I

A

S

S

L

A

G

V

T

C

S

K

K

I

I

N
|
N

I

V

D

N

S

T

D
x
E

G

N

R

Q

L

I

A
x
E

F

F

T

T

A

K

K

A

V

V

R

R

E

E

Y

V

L

L

A

N

N

K

N

D

P

Q

K

E

E

V

F

Y

D

D

P

P

R

R

K

K

Y

F

L

I

K

G

P

P

A

G

R

R

E

-

E

D

L

A

V

I

K

K

L

A

Y

T

K

V

H

I

K

G

N

K

I

I

N

R

V

E

L

F

G

G

S

S

A

N

G

G

K

K

A

A

active site: D85 (= D94), H86 (= H95), E145 (= E154), H181 (= H190), H209 (= H226), N231 (= N269)
binding zinc ion: H86 (= H95), E114 (= E123), H163 (≠ E172), H181 (= H190), H209 (= H226), E235 (≠ D273), E239 (≠ A277)

3n9sA Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with n-(4-hydroxybutyl)- glycolohydroxamic acid bis- phosphate, a competitive inhibitor (see paper)
44% identity, 97% coverage: 10:323/324 of query aligns to 2:306/307 of 3n9sA

query
sites
3n9sA

L

L

V

V

N

K

T

G

R

N

E

E

M

I

F

L

K

L

K

K

A

A

M

H

E

K

G

E

K

G

Y

Y

A

G

V

V

P

G

A

A

Y

F

N
|
N

F

F

N

V

N

N

L

F

E

E

Q

M

L

L

Q

N

A

A

I

I

I

F

Q

E

A

A

C

G

V

N

E

E

T

E

K

N

S

S

P

P

V

L

I

F

L

I

Q

Q

V

A

S
|
S

K

E

G

G

A

A

R

I

E

K

Y

Y

A

M

N

G

A

I

T

D

I

-

L

-

R

-

Y

-

L

M

A

A

L

V

G

G

A

M

T

V

Q

K

L

I

A

M

E
x
C

E
|
E

M

R

G

Y

H

P

K

H

I

I

P

P

I

V

A

A

L

L

H

H

L

L

D
|
D

H
|
H

G

G

D

T

S

T

F

F

E

E

I

S

C

C

K

E

S

K

C

A

V

V

D

K

Y

A

G

G

F

F

S

T

S

S

V

V

M

M

I

I

D
|
D

G

A

S
|
S

H

H

L

H

P

A

F

F

E

E

N

N

I

L

A

E

V

L

T

T

R

S

K

K

V

V

E

K

Y

M

A

A

H

H

Q

N

F

A

D

G

V

V

T

S

V

V

E

E

G

A

E
|
E

L

L

G
|
G

V

R

L

L

A

M

G

G

I

I

E

E

D

D

D

N

V

I

S

S

A

V

E

D

H

E

S

K

H

D

-

A

-

V

Y

L

T

V

D

N

P

P

A

K

Q

E

V

A

E

E

E

Q

F

F

V

V

A

K

K

E

T

S

G

Q

V

V

D

D

S

Y

L

L

A

A

I

P

S

A

I

I

G

G

T

T

S

S

H
|
H

G
|
G

A

A

Y

F

K
|
K

F

F

K

K

V

-

K

-

P

-

G

G

E

E

E

-

V

-

P

P

P

K

L

L

R

D

F

F

D

E

I

R

L

L

E

Q

E

E

V

V

E

-

K

K

R

R

L

L

P

T

G

N

F

I

P

P

I

L

V

V

L

L

H
|
H

G
|
G

A

A

S
|
S

S

A

V

I

V

P

P

D

E

N

Y

V

V

R

E

K

L

S

I

Y

N

L

K

D

Y
x
A

G

G

K

D

L

L

E

K

G

G

A

S

V

K

G

G

V

V

P

P

E

F

E

E

Q

F

L

L

R

Q

R

E

A

S

A

V

A

K

S

G

A

G

V

I

C

N

K

K

I

V

N
|
N

I

T

D
|
D

S
x
T

D

D

G

L

R

R

L

I

A

A

F

F

T

I

A

A

K

E

V

V

R

R

E

K

Y

V

L

A

A

N

N

E

N

D

P

K

K

S

E

Q

F

F

D

D

P

L

R

R

K

K

Y

F

L

F

K

S

P

P

A

A

R

Q

E

L

E

A

L

L

V

K

K

N

L

V

Y

V

K

K

H

E

K

R

N

-

I

M

N

K

V

L

L

L

G

G

S

S

A

A

G

N

K

K

active site: C69 (≠ E81), E70 (= E82), G136 (= G148), H180 (= H190), A226 (≠ Y242), N253 (= N269)
binding calcium ion: D104 (= D116), S106 (= S118), E134 (= E146)
binding 4-{hydroxy[(phosphonooxy)acetyl]amino}butyl dihydrogen phosphate: N23 (= N31), S49 (= S57), D82 (= D94), H83 (= H95), H180 (= H190), G181 (= G191), K184 (= K194), H210 (= H226), G211 (= G227), S213 (= S229), N253 (= N269), D255 (= D271), T256 (≠ S272)
binding zinc ion: H83 (= H95), H180 (= H190), H210 (= H226)

P13243 Probable fructose-bisphosphate aldolase; FBP aldolase; FBPA; Fructose-1,6-bisphosphate aldolase; EC 4.1.2.13 from Bacillus subtilis (strain 168) (see paper)
44% identity, 92% coverage: 10:306/324 of query aligns to 3:268/285 of P13243

query
sites
P13243

L

L

V

V

N

S

T

M

R

T

E

E

M

M

F

L

K

N

K

T

A

A

M

K

E

E

G

K

K

G

Y

Y

A

A

V

V

P

G

A

Q

Y

F

N

N

F

L

N

N

L

L

E

E

Q

F

L

T

Q

Q

A

A

I

I

I

L

Q

Q

A

A

C

A

V

E

E

E

T

E

K

K

S

S

P

P

V

V

I

I

L

L

Q

G

V

V

S

S

K

E

G

G

A

A

R

G

E

R

Y

Y

A

M

N

G

A

G

T

-

I

-

L

F

R

K

Y

T

L

V

A

V

L

A

G

M

A

V

T

K

Q

A

L

L

A

M

E

E

M

Y

G

K

H

V

K

T

I

V

P

P

I

V

A

A

L

I

H

H

L

L

D

D

H

H

G

G

D

S

S

S

F

F

E

E

I

S

C

C

K

A

S

K

C

A

V

I

D

H

Y

A

G

G

F

F

S

T

S

S

V

V

M

M

I

I

D

D

G

A

S

S

H

H

L

H

P

P

F

F

E

E

N

N

I

V

A

A

V

T

T

T

R

A

K

K

V

V

E

E

Y

L

A

A

H

H

Q

F

F

H

D

G

V

V

T

S

V

V

E

E

G

A

E

E

L

L

G

G

V

T

L

V

A

G

G

G

I

Q

E

E

D

D

V

V

S

I

A

A

E

E

H

G

S

V

H

I

Y

Y

T

A

D

D

P

P

A

K

Q

E

V

C

E

Q

E

E

F

L

V

V

A

E

K

R

T

T

G

G

V

I

D

D

S

C

L

L

A

A

I

P

S

A

I

L

G

G

T

S

S

V

H

H

G

G

A

P

Y

Y

K

K

F

-

K

-

V

-

K

-

P

-

G

G

E

E

E

-

V

-

P

P

P

N

L

L

R

G

F

F

D

K

I

E

L

M

E

E

V

I

E

G

K

K

R

S

L

T

P

-

G

G

F

L

P

P

I

L

V

V

L

L

H

H

G

G

A

G

S
x
T

S

-

V

-

P

-

E

-

Y

-

V

-

E

-

L

-

I

-

N

-

K

-

Y

-

G

-

K

-

L

-

E

-

G

-

A

-

V

-

G

G

V

I

P

P

E

T

E

A

Q

D

L

I

R

K

A

S

A

I

A

S

S

L

A

G

V

T

C

A

K

K

I

I

N

N

I

V

D

N

S
x
T

D

E

G

N

R

Q

L

I

A

S

F

S

T

A

A

K

K

A

V

V

R

R

E

E

Y

T

L

L

A

A

N

A

N

K

P

P

K

D

E

E

F

Y

D

D

P

P

R

R

K

K

Y

Y

L

L

K

G

P

P

A

A

R

R

E

E

E

A

L

I

T212 (≠ S229) modified: Phosphothreonine
T234 (≠ S272) modified: Phosphothreonine

4to8A Methicillin-resistant staphylococcus aureus class iib fructose 1,6- bisphosphate aldolase (see paper)
41% identity, 97% coverage: 10:324/324 of query aligns to 2:277/279 of 4to8A

query
sites
4to8A

L

L

V

V

N

S

T

M

R

K

E

E

M

M

F

L

K

I

K

D

A

A

M

K

E

E

G

N

K

G

Y

Y

A

A

V

V

P

G

A

Q

Y

Y

N

N

F

I

N

N

L

L

E

E

Q

F

L

T

Q

Q

A

A

I

I

I

L

Q

E

A

A

C

S

V

Q

E

E

T

E

K

N

S

A

P

P

V

V

I

I

L

L

Q

G

V

V

S

S

K

E

G

G

A

A

R

A

E

R

Y

Y

A

M

N

S

A

G

-

F

-

Y

T

T

I

I

L

V

R

K

Y

M

L

V

A

-

L

-

G

-

A

-

T

E

Q

G

L

L

A

M

E

H

E

D

M

L

G

N

H

I

K

T

I

I

P

P

I

V

A

A

L

I

H

H

L

L

D
|
D

H
|
H

G

G

D

S

S

S

F

F

E

E

I

K

C

C

K

K

S

E

C

A

V

I

D

D

Y

A

G

G

F

F

S

T

S

S

V

V

M

M

I

I

D

D

G

A

S

S

H

H

L

S

P

P

F

F

E

E

N

N

I

V

A

A

V

T

T

T

R

K

K

K

V

V

E

E

Y

Y

A

A

H

H

Q

E

F

K

D

G

V

V

T

S

V

V

E

E

G

A

E

E

L

L

G

G

V

T

L

V

A

G

G

G

I

-

E

-

D

D

V

V

S

V

A

A

E

D

H

G

S

I

H

I

Y

Y

T

A

D

D

P

P

A

K

Q

E

V

C

E

Q

E

E

F

L

V

V

A

E

K

K

T

T

G

G

V

I

D

D

S

A

L

L

A

A

I

P

S

A

I

L

G

G

T

S

S

V

H
|
H

G

G

A

-

Y

-

K

-

F

-

K

-

V

-

K

-

P

-

G

-

E

-

V

-

P

P

P

K

L

L

R

G

F

F

D

K

I

E

L

M

E

E

V

I

E

G

K

L

R

S

L

T

P

-

G

G

F

L

P

P

I

L

V

V

L

L

H
|
H

G

G

A

G

S

T

S

-

V

-

P

-

E

-

Y

-

V

-

E

-

L

-

I

-

N

-

K

-

Y

-

G

-

K

-

L

-

E

-

G

-

A

-

V

-

G

G

V

I

P

P

E

T

E

K

Q

D

L

I

R

Q

R

K

A

A

A

I

A

P

S

F

A

G

V

T

C

A

K

K

I

I

N
|
N

I

V

D

N

S

T

D

E

G

N

R

Q

L

I

A

A

F

S

T

A

A

K

K

A

V

V

R

R

E

D

Y

V

L

L

A

N

N

N

N

D

P

K

K

E

E

V

F

Y

D

D

P

P

R

R

K

K

Y

Y

L

L

K

G

P

P

A

A

R

R

E

E

E

A

L

I

V

K

K

E

L

T

Y

V

K

K

H

G

K

K

N

-

I

I

N

K

V

E

L

F

G

G

S

T

A

S

G

N

K

R

A

A

active site: D84 (= D94), H85 (= H95), H178 (= H190), H201 (= H226), N223 (= N269)
binding zinc ion: H85 (= H95), H201 (= H226)

3n9rA Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with n-(4-hydroxybutyl)-phosphoglycolohydroxamic acid, a competitive inhibitor (see paper)
43% identity, 97% coverage: 10:323/324 of query aligns to 2:296/297 of 3n9rA

query
sites
3n9rA

L

L

V

V

N

K

T

G

R

N

E

E

M

I

F

L

K

L

K

K

A

A

M

H

E

K

G

E

K

G

Y

Y

A

G

V

V

P

G

A

A

Y

F

N

N

F

F

N

V

N

N

L

F

E

E

Q

M

L

L

Q

N

A

A

I

I

I

F

Q

E

A

A

C

G

V

N

E

E

T

E

K

N

S

S

P

P

V

L

I

F

L

I

Q

Q

V

A

S

S

K

E

G

G

A

A

R

I

E

K

Y

Y

A

M

N

G

A

I

T

D

I

-

L

-

R

-

Y

-

L

M

A

A

L

V

G

G

A

M

T

V

Q

K

L

I

A

M

E
x
C

E
|
E

M

R

G

Y

H

P

K

H

I

I

P

P

I

V

A

A

L

L

H

H

L

L

D

D

H
|
H

G

G

D

T

S

T

F

F

E

E

I

S

C

C

K

E

S

K

C

A

V

V

D

K

Y

A

G

G

F

F

S

T

S

S

V

V

M

M

I

I

D

D

G

A

S

S

H

H

L

H

P

A

F

F

E

E

N

N

I

L

A

E

V

L

T

T

R

S

K

K

V

V

E

K

Y

M

A

A

H

H

Q

N

F

A

D

G

V

V

T

S

V

V

E

E

G

A

E

E

L

L

G
|
G

V

R

L

L

A

M

G

G

I

I

E

-

D

-

V

-

S

-

A

-

E

-

H

-

S

A

H

V

Y

L

T

V

D

N

P

P

A

K

Q

E

V

A

E

E

E

Q

F

F

V

V

A

K

K

E

T

S

G

Q

V

V

D

D

S

Y

L

L

A

A

I

P

S

A

I

I

G

G

T

T

S

S

H
|
H

G
|
G

A

A

Y

F

K
|
K

F

F

K

K

V

-

K

-

P

-

G

G

E

E

E

-

V

-

P

P

P

K

L

L

R

D

F

F

D

E

I

R

L

L

E

Q

E

E

V

V

E

-

K

K

R

R

L

L

P

T

G

N

F

I

P

P

I

L

V

V

L

L

H
|
H

G
|
G

A

A

S
|
S

S

A

V

I

V

P

P

D

E

N

Y

V

V

R

E

K

L

S

I

Y

N

L

K

D

Y
x
A

G

G

K

D

L

L

E

K

G

G

A

S

V

K

G

G

V

V

P

P

E

F

E

E

Q

F

L

L

R

Q

R

E

A

S

A

V

A

K

S

G

A

G

V

I

C

N

K

K

I

V

N
|
N

I

T

D
|
D

S
x
T

D

D

G

L

R

R

L

I

A

A

F

F

T

I

A

A

K

E

V

V

R

R

E

K

Y

V

L

A

A

N

N

E

N

D

P

K

K

S

E

Q

F

F

D

D

P

L

R

R

K

K

Y

F

L

F

K

S

P

P

A

A

R

Q

E

L

E

A

L

L

V

K

K

N

L

V

Y

V

K

K

H

E

K

R

N

-

I

M

N

K

V

L

L

L

G

G

S

S

A

A

G

N

K

K

active site: C69 (≠ E81), E70 (= E82), G136 (= G148), H170 (= H190), A216 (≠ Y242), N243 (= N269)
binding 2-[hydroxy(4-hydroxybutyl)amino]-2-oxoethyl dihydrogen phosphate: H83 (= H95), H170 (= H190), G171 (= G191), K174 (= K194), H200 (= H226), G201 (= G227), S203 (= S229), N243 (= N269), D245 (= D271), T246 (≠ S272)
binding zinc ion: H83 (= H95), H170 (= H190), H200 (= H226)

3c56A Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with n-(3-hydroxypropyl)-glycolohydroxamic acid bisphosphate, a competitive inhibitor (see paper)
43% identity, 97% coverage: 10:323/324 of query aligns to 2:296/297 of 3c56A

query
sites
3c56A

L

L

V

V

N

K

T

G

R

N

E

E

M

I

F

L

K

L

K

K

A

A

M

H

E

K

G

E

K

G

Y

Y

A

G

V

V

P

G

A

A

Y

F

N
|
N

F

F

N

V

N

N

L

F

E

E

Q

M

L

L

Q

N

A

A

I

I

I

F

Q

E

A

A

C

G

V

N

E

E

T

E

K

N

S

S

P

P

V

L

I

F

L

I

Q

Q

V

A

S
|
S

K

E

G

G

A

A

R

I

E

K

Y

Y

A

M

N

G

A

I

T

D

I

-

L

-

R

-

Y

-

L

M

A

A

L

V

G

G

A

M

T

V

Q

K

L

I

A

M

E
x
C

E
|
E

M

R

G

Y

H

P

K

H

I

I

P

P

I

V

A

A

L

L

H

H

L

L

D
|
D

H
|
H

G

G

D

T

S

T

F

F

E

E

I

S

C

C

K

E

S

K

C

A

V

V

D

K

Y

A

G

G

F

F

S

T

S

S

V

V

M

M

I

I

D

D

G

A

S

S

H

H

L

H

P

A

F

F

E

E

N

N

I

L

A

E

V

L

T

T

R

S

K

K

V

V

E

K

Y

M

A

A

H

H

Q

N

F

A

D

G

V

V

T

S

V

V

E

E

G

A

E

E

L

L

G
|
G

V

R

L

L

A

M

G

G

I

I

E

-

D

-

V

-

S

-

A

-

E

-

H

-

S

A

H

V

Y

L

T

V

D

N

P

P

A

K

Q

E

V

A

E

E

E

Q

F

F

V

V

A

K

K

E

T

S

G

Q

V

V

D

D

S

Y

L

L

A

A

I

P

S

A

I

I

G

G

T

T

S

S

H
|
H

G

G

A

A

Y

F

K
|
K

F

F

K

K

V

-

K

-

P

-

G

G

E

E

E

-

V

-

P

P

P

K

L

L

R

D

F

F

D

E

I

R

L

L

E

Q

E

E

V

V

E

-

K

K

R

R

L

L

P

T

G

N

F

I

P

P

I

L

V

V

L

L

H
|
H

G
|
G

A

A

S
|
S

S

A

V

I

V

P

P

D

E

N

Y

V

V

R

E

K

L

S

I

Y

N

L

K

D

Y
x
A

G

G

K

D

L

L

E

K

G

G

A

S

V

K

G

G

V

V

P

P

E

F

E

E

Q

F

L

L

R

Q

R

E

A

S

A

V

A

K

S

G

A

G

V

I

C

N

K

K

I

V

N
|
N

I

T

D
|
D

S
x
T

D

D

G

L

R
|
R

L

I

A

A

F

F

T

I

A

A

K

E

V

V

R

R

E

K

Y

V

L

A

A

N

N

E

N

D

P

K

K

S

E

Q

F

F

D

D

P

L

R

R

K

K

Y

F

L

F

K

S

P

P

A

A

R

Q

E

L

E

A

L

L

V

K

K

N

L

V

Y

V

K

K

H

E

K

R

N

-

I

M

N

K

V

L

L

L

G

G

S

S

A

A

G

N

K

K

active site: C69 (≠ E81), E70 (= E82), G136 (= G148), H170 (= H190), A216 (≠ Y242), N243 (= N269)
binding 3-{hydroxy[(phosphonooxy)acetyl]amino}propyl dihydrogen phosphate: N23 (= N31), S49 (= S57), D82 (= D94), H170 (= H190), K174 (= K194), G201 (= G227), S203 (= S229), N243 (= N269), D245 (= D271), T246 (≠ S272), R249 (= R275)
binding zinc ion: H83 (= H95), H170 (= H190), H200 (= H226)

3c52A Class ii fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with phosphoglycolohydroxamic acid, a competitive inhibitor (see paper)
43% identity, 97% coverage: 10:323/324 of query aligns to 2:295/296 of 3c52A

query
sites
3c52A

L

L

V

V

N

K

T

G

R

N

E

E

M

I

F

L

K

L

K

K

A

A

M

H

E

K

G

E

K

G

Y

Y

A

G

V

V

P

G

A

A

Y

F

N

N

F

F

N

V

N

N

L

F

E

E

Q

M

L

L

Q

N

A

A

I

I

I

F

Q

E

A

A

C

G

V

N

E

E

T

E

K

N

S

S

P

P

V

L

I

F

L

I

Q

Q

V

A

S

S

K

E

G

G

A

A

R

I

E

K

Y

Y

A

M

N

G

A

I

T

D

I

-

L

-

R

-

Y

-

L

M

A

A

L

V

G

G

A

M

T

V

Q

K

L

I

A

M

E
x
C

E
|
E

M

R

G

Y

H

P

K

H

I

I

P

P

I

V

A

A

L

L

H

H

L

L

D
|
D

H
|
H

G

G

D

T

S

T

F

F

E

E

I

S

C

C

K

E

S

K

C

A

V

V

D

K

Y

A

G

G

F

F

S

T

S

S

V

V

M

M

I

I

D
|
D

G

A

S
|
S

H

H

L

H

P

A

F

F

E

E

N

N

I

L

A

E

V

L

T

T

R

S

K

K

V

V

E

K

Y

M

A

A

H

H

Q

N

F

A

D

G

V

V

T

S

V

V

E

E

G

A

E
|
E

L

L

G
|
G

V

R

L

L

A

M

G

G

I

I

E

V

D

-

V

-

S

-

A

-

E

-

H

-

S

-

H

-

Y

L

T

V

D

N

P

P

A

K

Q

E

V

A

E

E

E

Q

F

F

V

V

A

K

K

E

T

S

G

Q

V

V

D

D

S

Y

L

L

A

A

I

P

S

A

I

I

G

G

T

T

S

S

H
|
H

G

G

A

A

Y

F

K
|
K

F

F

K

K

V

-

K

-

P

-

G

G

E

E

E

-

V

-

P

P

P

K

L

L

R

D

F

F

D

E

I

R

L

L

E

Q

E

E

V

V

E

-

K

K

R

R

L

L

P

T

G

N

F

I

P

P

I

L

V

V

L

L

H
|
H

G
|
G

A

A

S
|
S

S

A

V

I

V

P

P

D

E

N

Y

V

V

R

E

K

L

S

I

Y

N

L

K

D

Y
x
A

G

G

K

D

L

L

E

K

G

G

A

S

V

K

G

G

V

V

P

P

E

F

E

E

Q

F

L

L

R

Q

R

E

A

S

A

V

A

K

S

G

A

G

V

I

C

N

K

K

I

V

N
|
N

I

T

D
|
D

S
x
T

D

D

G

L

R

R

L

I

A

A

F

F

T

I

A

A

K

E

V

V

R

R

E

K

Y

V

L

A

A

N

N

E

N

D

P

K

K

S

E

Q

F

F

D

D

P

L

R

R

K

K

Y

F

L

F

K

S

P

P

A

A

R

Q

E

L

E

A

L

L

V

K

K

N

L

V

Y

V

K

K

H

E

K

R

N

-

I

M

N

K

V

L

L

L

G

G

S

S

A

A

G

N

K

K

active site: C69 (≠ E81), E70 (= E82), G136 (= G148), H169 (= H190), A215 (≠ Y242), N242 (= N269)
binding calcium ion: D104 (= D116), S106 (= S118), E134 (= E146)
binding phosphoglycolohydroxamic acid: D82 (= D94), H83 (= H95), H169 (= H190), K173 (= K194), H199 (= H226), G200 (= G227), S202 (= S229), N242 (= N269), D244 (= D271), T245 (≠ S272)
binding zinc ion: H83 (= H95), H169 (= H190), H199 (= H226)

5uckA Class ii fructose-1,6-bisphosphate aldolase of helicobacter pylori with cleavage products (see paper)
43% identity, 97% coverage: 10:323/324 of query aligns to 2:290/291 of 5uckA

query
sites
5uckA

L

L

V

V

N

K

T

G

R

N

E

E

M

I

F

L

K

L

K

K

A

A

M

H

E

K

G

E

K

G

Y

Y

A

G

V

V

P

G

A

A

Y

F

N

N

F

F

N

V

N

N

L

F

E

E

Q

M

L

L

Q

N

A

A

I

I

I

F

Q

E

A

A

C

G

V

N

E

E

T

E

K

N

S

S

P

P

V

L

I

F

L

I

Q

Q

V

A

S
|
S

K

E

G

G

A

A

R

I

E

K

Y

Y

A

M

N

G

A

I

T

D

I

-

L

-

R

-

Y

-

L

M

A

A

L

V

G

G

A

M

T

V

Q

K

L

I

A

M

E

C

E

E

M

R

G

Y

H

P

K

H

I

I

P

P

I

V

A

A

L

L

H

H

L

L

D
|
D

H
|
H

G

G

D

T

S

T

F

F

E

E

I

S

C

C

K

E

S

K

C

A

V

V

D

K

Y

A

G

G

F

F

S

T

S

S

V

V

M

M

I

I

D

D

G

A

S

S

H

H

L

H

P

A

F

F

E

E

N

N

I

L

A

E

V

L

T

T

R

S

K

K

V

V

E

K

Y

M

A

A

H

H

Q

N

F

A

D

G

V

V

T

S

V

V

E

E

G

A

E
|
E

L

L

G

G

V

R

L

L

A

-

G

-

I

-

E

-

D

-

V

-

S

-

A

-

E

-

H

-

S

-

H

-

Y

-

T

V

D

N

P

P

A

K

Q

E

V

A

E

E

E

Q

F

F

V

V

A

K

K

E

T

S

G

Q

V

V

D

D

S

Y

L

L

A

A

I

P

S

A

I

I

G

G

T

T

S

S

H
|
H

G

G

A

A

Y

F

K

K

F

F

K

K

V

-

K

-

P

-

G

G

E

E

E

-

V

-

P

P

P

K

L

L

R

D

F

F

D

E

I

R

L

L

E

Q

E

E

V

V

E

-

K

K

R

R

L

L

P

T

G

N

F

I

P

P

I

L

V

V

L

L

H
|
H

G

G

A

A

S

S

S

A

V

I

V

P

P

D

E

N

Y

V

V

R

E

K

L

S

I

Y

N

L

K

D

Y

A

G

G

K

D

L

L

E

K

G

G

A

S

V

K

G

G

V

V

P

P

E

F

E

E

Q

F

L

L

R

Q

R

E

A

S

A

V

A

K

S

G

A

G

V

I

C

N

K

K

I

V

N

N

I

T

D
|
D

S

T

D

D

G

L

R
|
R

L

I

A

A

F

F

T

I

A

A

K

E

V

V

R

R

E

K

Y

V

L

A

A

N

N

E

N

D

P

K

K

S

E

Q

F

F

D

D

P

L

R

R

K

K

Y

F

L

F

K

S

P

P

A

A

R

Q

E

L

E

A

L

L

V

K

K

N

L

V

Y

V

K

K

H

E

K

R

N

-

I

M

N

K

V

L

L

L

G

G

S

S

A

A

G

N

K

K

binding glyceraldehyde-3-phosphate: S49 (= S57), D82 (= D94), H83 (= H95), H164 (= H190), D239 (= D271), R243 (= R275)
binding zinc ion: H83 (= H95), H83 (= H95), E134 (= E146), H164 (= H190), H194 (= H226), H194 (= H226)

5ucpA Class ii fructose-1,6-bisphosphate aldolase e142a variant of helicobacter pylori with fbp and cleavage products (see paper)
42% identity, 97% coverage: 10:323/324 of query aligns to 2:291/292 of 5ucpA

query
sites
5ucpA

L

L

V

V

N

K

T

G

R

N

E

E

M

I

F

L

K

L

K

K

A

A

M

H

E

K

G

E

K

G

Y

Y

A

G

V

V

P

G

A

A

Y

F

N

N

F

F

N

V

N

N

L

F

E

E

Q

M

L

L

Q

N

A

A

I

I

I

F

Q

E

A

A

C

G

V

N

E

E

T

E

K

N

S

S

P

P

V

L

I

F

L

I

Q

Q

V

A

S
|
S

K

E

G

G

A

A

R

I

E

K

Y

Y

A

M

N

G

A

I

T

D

I

-

L

-

R

-

Y

-

L

M

A

A

L

V

G

G

A

M

T

V

Q

K

L

I

A

M

E

C

E

E

M

R

G

Y

H

P

K

H

I

I

P

P

I

V

A

A

L

L

H

H

L

L

D
|
D

H
|
H

G

G

D

T

S

T

F

F

E

E

I

S

C

C

K

E

S

K

C

A

V

V

D

K

Y

A

G

G

F

F

S

T

S

S

V

V

M

M

I

I

D

D

G

A

S

S

H

H

L

H

P

A

F

F

E

E

N

N

I

L

A

E

V

L

T

T

R

S

K

K

V

V

E

K

Y

M

A

A

H

H

Q

N

F

A

D

G

V

V

T

S

V

V

E

E

G

A

E
|
E

L

L

G

G

V

R

L

V

A

-

G

-

I

-

E

-

D

-

V

-

S

-

A

-

E

-

H

-

S

-

H

-

Y

L

T

V

D

N

P

P

A

K

Q

E

V

A

E

E

E

Q

F

F

V

V

A

K

K

E

T

S

G

Q

V

V

D

D

S

Y

L

L

A

A

I

P

S

A

I

I

G

G

T

T

S

S

H
|
H

G

G

A

A

Y

F

K
|
K

F

F

K

K

V

-

K

-

P

-

G

G

E

E

E

-

V

-

P

P

P

K

L

L

R

D

F

F

D

E

I

R

L

L

E

Q

E

E

V

V

E

-

K

K

R

R

L

L

P

T

G

N

F

I

P

P

I

L

V

V

L

L

H
|
H

G
|
G

A

A

S
|
S

S

A

V

I

V

P

P

D

E

N

Y

V

V

R

E

K

L

S

I

Y

N

L

K

D

Y

A

G

G

K

D

L

L

E

K

G

G

A

S

V

K

G

G

V

V

P

P

E

F

E

E

Q

F

L

L

R

Q

R

E

A

S

A

V

A

K

S

G

A

G

V

I

C

N

K

K

I

V

N
|
N

I

T

D
|
D

S
x
T

D

D

G

L

R
|
R

L

I

A

A

F

F

T

I

A

A

K

E

V

V

R

R

E

K

Y

V

L

A

A

N

N

E

N

D

P

K

K

S

E

Q

F

F

D

D

P

L

R

R

K

K

Y

F

L

F

K

S

P

P

A

A

R

Q

E

L

E

A

L

L

V

K

K

N

L

V

Y

V

K

K

H

E

K

R

N

-

I

M

N

K

V

L

L

L

G

G

S

S

A

A

G

N

K

K

binding 1,6-di-O-phosphono-D-fructose: S49 (= S57), D82 (= D94), H83 (= H95), H165 (= H190), K169 (= K194), G196 (= G227), S198 (= S229), N238 (= N269), D240 (= D271), T241 (≠ S272), R244 (= R275)
binding zinc ion: H83 (= H95), H83 (= H95), H83 (= H95), E134 (= E146), H165 (= H190), H165 (= H190), H165 (= H190), H195 (= H226), H195 (= H226)

5ud4A Class ii fructose-1,6-bisphosphate aldolase h180q variant of helicobacter pylori with tbp (see paper)
42% identity, 97% coverage: 10:323/324 of query aligns to 2:292/293 of 5ud4A

query
sites
5ud4A

L

L

V

V

N

K

T

G

R

N

E

E

M

I

F

L

K

L

K

K

A

A

M

H

E

K

G

E

K

G

Y

Y

A

G

V

V

P

G

A

A

Y

F

N

N

F

F

N

V

N

N

L

F

E

E

Q

M

L

L

Q

N

A

A

I

I

I

F

Q

E

A

A

C

G

V

N

E

E

T

E

K

N

S

S

P

P

V

L

I

F

L

I

Q

Q

V

A

S
|
S

K

E

G

G

A

A

R

I

E

K

Y

Y

A

M

N

G

A

I

T

D

I

-

L

-

R

-

Y

-

L

M

A

A

L

V

G

G

A

M

T

V

Q

K

L

I

A

M

E

C

E

E

M

R

G

Y

H

P

K

H

I

I

P

P

I

V

A

A

L

L

H

H

L

L

D
|
D

H
|
H

G

G

D

T

S

T

F

F

E

E

I

S

C

C

K

E

S

K

C

A

V

V

D

K

Y

A

G

G

F

F

S

T

S

S

V

V

M

M

I

I

D

D

G

A

S

S

H

H

L

H

P

A

F

F

E

E

N

N

I

L

A

E

V

L

T

T

R

S

K

K

V

V

E

K

Y

M

A

A

H

H

Q

N

F

A

D

G

V

V

T

S

V

V

E

E

G

A

E
|
E

L

L

G

G

V

R

L

L

A

V

G

-

I

-

E

-

D

-

V

-

S

-

A

-

E

-

H

-

S

-

H

-

Y

L

T

V

D

N

P

P

A

K

Q

E

V

A

E

E

E

Q

F

F

V

V

A

K

K

E

T

S

G

Q

V

V

D

D

S

Y

L

L

A

A

I

P

S

A

I

I

G

G

T

T

S

S

H
x
Q

G
|
G

A

A

Y

F

K
|
K

F

F

K

K

V

-

K

-

P

-

G

G

E

E

E

-

V

-

P

P

P

K

L

L

R

D

F

F

D

E

I

R

L

L

E

Q

E

E

V

V

E

-

K

K

R

R

L

L

P

T

G

N

F

I

P

P

I

L

V

V

L

L

H
|
H

G
|
G

A

A

S
|
S

S

A

V

I

V

P

P

D

E

N

Y

V

V

R

E

K

L

S

I

Y

N

L

K

D

Y

A

G

G

K

D

L

L

E

K

G

G

A

S

V

K

G

G

V

V

P

P

E

F

E

E

Q

F

L

L

R

Q

R

E

A

S

A

V

A

K

S

G

A

G

V

I

C

N

K

K

I

V

N
|
N

I

T

D
|
D

S
x
T

D

D

G

L

R
|
R

L

I

A

A

F

F

T

I

A

A

K

E

V

V

R

R

E

K

Y

V

L

A

A

N

N

E

N

D

P

K

K

S

E

Q

F

F

D

D

P

L

R

R

K

K

Y

F

L

F

K

S

P

P

A

A

R

Q

E

L

E

A

L

L

V

K

K

N

L

V

Y

V

K

K

H

E

K

R

N

-

I

M

N

K

V

L

L

L

G

G

S

S

A

A

G

N

K

K

binding 1,6-di-O-phosphono-D-tagatose: S49 (= S57), D82 (= D94), Q166 (≠ H190), G167 (= G191), K170 (= K194), G197 (= G227), S199 (= S229), N239 (= N269), D241 (= D271), T242 (≠ S272), R245 (= R275)
binding zinc ion: H83 (= H95), H83 (= H95), E134 (= E146), H196 (= H226), H196 (= H226)

5ud0A Class ii fructose-1,6-bisphosphate aldolase e149a variant of helicobacter pylori with cleavage products (see paper)
40% identity, 97% coverage: 10:323/324 of query aligns to 2:281/282 of 5ud0A

query
sites
5ud0A

L

L

V

V

N

K

T

G

R

N

E

E

M

I

F

L

K

L

K

K

A

A

M

H

E

K

G

E

K

G

Y

Y

A

G

V

V

P

G

A

A

Y

F

N

N

F

F

N

V

N

N

L

F

E

E

Q

M

L

L

Q

N

A

A

I

I

I

F

Q

E

A

A

C

G

V

N

E

E

T

E

K

N

S

S

P

P

V

L

I

F

L

I

Q

Q

V

A

S

S

K

E

G

G

A

A

R

I

E

K

Y

Y

A

M

N

G

A

I

T

D

I

-

L

-

R

-

Y

-

L

M

A

A

L

V

G

G

A

M

T

V

Q

K

L

I

A

M

E

C

E

E

M

R

G

Y

H

P

K

H

I

I

P

P

I

V

A

A

L

L

H

H

L

L

D

D

H
|
H

G

G

D

T

S

T

F

F

E

E

I

S

C

C

K

E

S

K

C

A

V

V

D

K

Y

A

G

G

F

F

S

T

S

S

V

V

M

M

I

I

D

D

G

A

S

S

H

H

L

H

P

A

F

F

E

E

N

N

I

L

A

E

V

L

T

T

R

S

K

K

V

V

E

K

Y

M

A

A

H

H

Q

N

F

A

D

G

V

V

T

S

V

V

E

E

G

A

E
|
E

L

L

G

G

V

L

L

V

A

-

G

-

I

-

E

-

D

-

V

-

S

-

A

-

E

-

H

-

S

-

H

-

Y

-

T

-

D

N

P

P

A

K

Q

E

V

A

E

E

E

Q

F

F

V

V

A

K

K

E

T

S

G

Q

V

V

D

D

S

Y

L

L

A

A

I

P

S

A

I

I

G

G

T

T

S

S

H
|
H

G

G

A

-

Y

-

K

-

F

-

K

-

V

-

K

-

P

-

G

-

E

-

V

-

P

-

P

K

L

L

R

D

F

F

D

E

I

R

L

L

E

Q

E

E

V

V

E

-

K

K

R

R

L

L

P

T

G

N

F

I

P

P

I

L

V

V

L

L

H
|
H

G

G

A

A

S

S

S

A

V

I

V

P

P

D

E

N

Y

V

V

R

E

K

L

S

I

Y

N

L

K

D

Y

A

G

G

K

D

L

L

E

K

G

G

A

S

V

K

G

G

V

V

P

P

E

F

E

E

Q

F

L

L

R

Q

R

E

A

S

A

V

A

K

S

G

A

G

V

I

C

N

K

K

I

V

N

N

I

T

D

D

S

T

D

D

G

L

R

R

L

I

A

A

F

F

T

I

A

A

K

E

V

V

R

R

E

K

Y

V

L

A

A

N

N

E

N

D

P

K

K

S

E

Q

F

F

D

D

P

L

R

R

K

K

Y

F

L

F

K

S

P

P

A

A

R

Q

E

L

E

A

L

L

V

K

K

N

L

V

Y

V

K

K

H

E

K

R

N

-

I

M

N

K

V

L

L

L

G

G

S

S

A

A

G

N

K

K

binding zinc ion: H83 (= H95), E134 (= E146), H163 (= H190), H185 (= H226)

P0AB74 D-tagatose-1,6-bisphosphate aldolase subunit KbaY; TBPA; TagBP aldolase; D-tagatose-bisphosphate aldolase class II; Ketose 1,6-bisphosphate aldolase class II; Tagatose-bisphosphate aldolase; EC 4.1.2.40 from Escherichia coli (strain K12) (see paper)
36% identity, 98% coverage: 8:323/324 of query aligns to 1:283/286 of P0AB74

query
sites
P0AB74

L

M

G

S

L

I

V

I

N

S

T

T

R

K

E

Y

M

L

F

L

K

Q

K

D

A

A

M

Q

E

A

G

N

K

G

Y

Y

A

A

V

V

P

P

A

A

Y

F

N

N

F

I

N

H

N

N

L

A

E

E

Q

T

L

I

Q

Q

A

A

I

I

I

L

Q

E

A

V

C

C

V

S

E

E

T

M

K

R

S

S

P

P

V

V

I

I

L

L

Q

A

V

G

S

T

K

P

G

G

A

T

R

-

E

-

Y

-

A

-

N

-

A

-

T

-

I

-

L

F

R

K

Y

H

L

I

A

A

L

L

G

E

-

E

-

I

-

Y

A

A

T

L

Q

C

L

S

A

A

E

Y

E

S

M

T

G

T

H

Y

K

N

I

M

P

P

I

L

A

A

L

L

H

H

L

L

D
|
D

H
|
H

G

H

D

E

S

S

F

L

E

D

I

D

C

I

K

R

S

R

C

K

V

V

D

H

Y

A

G

G

F

V

S

R

S

S

V

A

M

M

I

I

D

D

G

G

S

S

H

H

L

F

P

P

F

F

E

A

E

E

N

N

I

V

A

K

V

L

T

V

R

K

K

S

V

V

E

D

Y

F

A

C

H

H

Q

S

F

Q

D

D

V

C

T

S

V

V

E

E

G

A

E

E

L

L

G

G

V

R

L

L

A

G

G

G

I

V

E

E

D

D

V

M

S

S

-

V

-

D

A

A

E

E

H

S

S

A

H

F

Y

L

T

T

D

D

P

P

A

Q

Q

E

V

A

E

K

E

R

F

F

V

V

A

E

K

L

T

T

G

G

V

V

D

D

S

S

L

L

A

A

I

V

S

A

I

I

G

G

T

T

S

A

H
|
H

G

G

A

L

Y

Y

K

S

F

-

K

-

V

-

K

-

P

-

G

-

E

-

E

K

V

T

P

P

P

K

L

I

R

D

F

F

D

Q

I

R

L

L

E

A

E

E

V

I

E

-

K

R

R

E

L

V

P

V

G

D

F

V

P

P

I

L

V

V

L

L

H
|
H

G

G

A

A

S

S

S

D

V

-

P

-

E

-

Y

-

V

-

E

-

L

-

I

-

N

-

K

-

Y

-

G

-

K

-

L

-

E

-

G

-

A

-

V

-

G

-

V

V

P

P

E

D

E

E

Q

F

L

V

R

R

A

T

A

I

A

E

S

L

A

G

V

V

C

T

K

K

I

V

N

N

I

V

D

A

S

T

D

E

G

L

R

K

L

I

A

A

F

F

T

A

A

G

K

A

V

V

R

K

E

A

Y

W

L

F

A

A

N

E

N

N

P

P

K

Q

E

G

F

N

D

D

P

P

R

R

K

Y

Y

Y

L

M

K

R

P

V

A

G

R

M

E

D

E

A

L

M

V

K

K

E

L

V

Y

V

K

R

H

N

K

K

N

-

I

I

N

N

V

V

L

C

G

G

S

S

A

A

G

N

K

R

D82 (= D94) active site, Proton donor
H83 (= H95) binding Zn(2+)
H180 (= H190) binding Zn(2+)
H208 (= H226) binding Zn(2+)

1gvfB Structure of tagatose-1,6-bisphosphate aldolase (see paper)
35% identity, 97% coverage: 10:323/324 of query aligns to 2:274/275 of 1gvfB

query
sites
1gvfB

L

I

V

I

N

S

T

T

R

K

E

Y

M

L

F

L

K

Q

K

D

A

A

M

Q

E

A

G

N

K

G

Y

Y

A

A

V

V

P

P

A

A

Y

F

N

N

F

I

N

H

N

N

L

A

E

E

Q

T

L

I

Q

Q

A

A

I

I

I

L

Q

E

A

V

C

C

V

S

E

E

T

M

K

R

S

S

P

P

V

V

I

I

L

L

Q

A

V

G

S

T

K

P

G

G

A

T

R

-

E

-

Y

-

A

-

N

-

A

-

T

-

I

-

L

F

R

K

Y

H

L

I

A

A

L

L

G

E

-

E

-

I

-

Y

A

A

T

L

Q

C

L

S

A

A

E

Y

E

S

M

T

G

T

H

Y

K

N

I

M

P

P

I

L

A

A

L

L

H

H

L

L

D
|
D

H
|
H

G

H

D

E

S

S

F

L

E

D

I

D

C

I

K

R

S

R

C

K

V

V

D

H

Y

A

G

G

F

V

S

R

S

S

V

A

M

M

I

I

D

D

G

G

S

S

H

H

L

F

P

P

F

F

E

A

E

E

N

N

I

V

A

K

V

L

T

V

R

K

K

S

V

V

E

D

Y

F

A

C

H

H

Q

S

F

Q

D

D

V

C

T

S

V

V

E

E

G

A

E

E

L

L

G

G

V

R

L

L

A

G

G

G

I

V

E

E

D

S

D

-

V

-

S

-

A

-

E

-

H

-

S

A

H

F

Y

L

T

T

D

D

P

P

A

Q

Q

E

V

A

E

K

E

R

F

F

V

V

A

E

K

L

T

T

G

G

V

V

D

D

S

S

L

L

A

A

I

V

S

A

I

I

G

G

T

T

S

A

H
|
H

G
|
G

A

L

Y

Y

K

S

F

-

K

-

V

-

K

-

P

-

G

-

E

-

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K

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T

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P

P

K

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I

R

D

F

F

D

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I

R

L

L

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A

E

E

V

I

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-

K

R

R

E

L

V

P

V

G

D

F

V

P

P

I

L

V

V

L

L

H
|
H

G
|
G

A

A

S
|
S

S

D

V

-

P

-

E

-

Y

-

V

-

E

-

L

-

I

-

N

-

K

-

Y

-

G

-

K

-

L

-

E

-

G

-

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-

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-

G

-

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V

P

P

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D

E

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Q

F

L

V

R

R

A

T

A

I

A

E

S

L

A

G

V

V

C

T

K

K

I

V

N
|
N

I
x
V

D
x
A

S
x
T

D

E

G

L

R

K

L

I

A

A

F

F

T

A

A

G

K

A

V

V

R

K

E

A

Y

W

L

F

A

A

N

E

N

N

P

P

K

Q

E

G

F

N

D

D

P

P

R

R

K

Y

Y

Y

L

M

K

R

P

V

A

G

R

M

E

D

E

A

L

M

V

K

K

E

L

V

Y

V

K

R

H

N

K

K

N

-

I

I

N

N

V

V

L

C

G

G

S

S

A

A

G

N

K

R

active site: D81 (= D94), H82 (= H95), H171 (= H190), H199 (= H226), N221 (= N269)
binding phosphoglycolohydroxamic acid: D81 (= D94), H82 (= H95), H171 (= H190), G172 (= G191), H199 (= H226), G200 (= G227), S202 (= S229), N221 (= N269), V222 (≠ I270), A223 (≠ D271), T224 (≠ S272)
binding zinc ion: H82 (= H95), H171 (= H190), H199 (= H226)

Query Sequence

>WP_013452043.1 NCBI__GCF_000183405.1:WP_013452043.1
MAVSYKELGLVNTREMFKKAMEGKYAVPAYNFNNLEQLQAIIQACVETKSPVILQVSKGA
REYANATILRYLALGATQLAEEMGHKIPIALHLDHGDSFEICKSCVDYGFSSVMIDGSHL
PFEENIAVTRKVVEYAHQFDVTVEGELGVLAGIEDDVSAEHSHYTDPAQVEEFVAKTGVD
SLAISIGTSHGAYKFKVKPGEEVPPLRFDILEEVEKRLPGFPIVLHGASSVVPEYVELIN
KYGGKLEGAVGVPEEQLRRAAASAVCKINIDSDGRLAFTAKVREYLANNPKEFDPRKYLK
PAREELVKLYKHKNINVLGSAGKA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory