SitesBLAST

3nz2C Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
29% identity, 70% coverage: 43:171/183 of query aligns to 45:175/183 of 3nz2C

query
sites
3nz2C

V

A

L

L

L

Q

L

R

R

E

I

L

F

F

G

G

A

-

K

H

I

L

G

G

K

H

N

K

V

S

V

C

I

V

K

Q

P

P

C

P

V

F

N

H

I

C

K

E

Y

F

P

G

W

K

F

T

L

I

K

R

I

I

G

G

D

D

N

H

V

T

W

F

I

I

G

N

E

M

N

N

V

V

W

V

I

M

D

L

N

D

L

G

T

A

T

P

V

I

E

T

I

I

G

G

N

D

N

H

V

V

C

L

I

I

S

G

Q

P

G

S

A

T

Y

Q

I

F

F
x
Y

T

T

G

A

N

S

H
|
H

N

S

Y

L

-

D

-

Y

-

R

K

R

T

R

Q

Q

S

A

F

W

D

E

L

T

I

I

I

C

K

K

P

P

V

I

I

V

I

I

E

E

D

D

G

D

V

V

W

W

I

I

G
|
G

A

G

K

N

A

V

I

V

V

I

C

N

P

Q

G

G

V

V

R

T

C

I

K

G

S

A

H

R

S

S

I

V

L

V

S
x
A

V
x
A

G
x
N

S

S

V

V

A

V

T
x
N

K

Q

D

D

L

V

E

P

Y

D

T

T

V

L

Y

V

Q
x
G

G

G

N
x
T

P
|
P

A

A

binding acetyl coenzyme *a: Y107 (≠ F106), H111 (= H110), G137 (= G133), A155 (≠ S151), A156 (≠ V152), N161 (≠ T157), G171 (≠ Q167), T173 (≠ N169), P174 (= P170)
binding magnesium ion: N157 (≠ G153), T173 (≠ N169)

Sites not aligning to the query:

binding acetyl coenzyme *a: 178, 179

3nz2J Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
29% identity, 70% coverage: 43:171/183 of query aligns to 48:178/185 of 3nz2J

query
sites
3nz2J

V

A

L

L

L

Q

L

R

R

E

I

L

F

F

G

G

A

-

K

H

I

L

G

G

K

H

N

K

V

S

V

C

I

V

K

Q

P

P

C

P

V

F

N

H

I

C

K

E

Y

F

P

G

W

K

F

T

L

I

K

R

I

I

G

G

D

D

N

H

V

T

W

F

I

I

G

N

E

M

N

N

V

V

W

V

I

M

D

L

N

D

L

G

T

A

T

P

V

I

E

T

I

I

G

G

N

D

N

H

V

V

C

L

I

I

S
x
G

Q

P

G

S

A

T

Y

Q

I

F

F
x
Y

T

T

G

A

N

S

H
|
H

N

S

Y

L

-

D

-

Y

-

R

K

R

T

R

Q

Q

S

A

F

W

D

E

L

T

I

I

I

C

K

K

P

P

V

I

I

V

I

I

E

E

D

D

G

D

V

V

W
|
W

I

I

G
|
G

A

G

K

N

A

V

I

V

V

I

C

N

P

Q

G

G

V

V

R

T

C

I

K

G

S

A

H

R

S

S

I

V

L

V

S
x
A

V
x
A

G

N

S

S

V

V

A

V

T
x
N

K

Q

D

D

L

V

E

P

Y

D

T

T

V

L

Y

V

Q
x
G

G

G

N
x
T

P
|
P

A

A

binding acetyl coenzyme *a: G104 (≠ S100), Y110 (≠ F106), H114 (= H110), W138 (= W131), G140 (= G133), A158 (≠ S151), A159 (≠ V152), N164 (≠ T157), G174 (≠ Q167), T176 (≠ N169), P177 (= P170)

Sites not aligning to the query:

binding acetyl coenzyme *a: 182

4isxA The crystal structure of maltose o-acetyltransferase from clostridium difficile 630 in complex with acetyl-coa
30% identity, 78% coverage: 29:171/183 of query aligns to 34:178/186 of 4isxA

query
sites
4isxA

L

L

F

F

F

N

K

N

T

T

S

L

I

E

P

D

Y

E

P

Y

S

E

K

K

I

R

K

E

V

D

L

I

L

L

L

R

R

Q

I

L

F

F

G

G

A

S

K

-

I

V

G

G

K

K

N

Q

V

I

V

N

I

V

K

E

P

Q

C

N

V

I

N

R

I

C

K

D

Y

Y

P

G

W

Y

F

N

L

I

K

H

I

V

G

G

D

E

N

N

V

F

W

F

I

A

G
x
N

E

Y

N

D

V

C

W

I

I

F

D

L

N

D

L

V

T

C

T

K

V

I

E

E

I

I

G

G

N

D

N

N

V

V

C

M

I

L

S
x
A

Q

P

G

N

A

V

Y

Q

I

I

F

Y

T

T

G

A

N

Y

H

H

N

P

Y

I

K

D

T

A

Q

Q

S

L

F

R

D

N

L

S

I

G

I

I

K

E

-

Y

-

G

-

S

P

P

V

V

I

K

I

I

E

G

D

D

G

N

V

V

W
|
W

I

I

G
|
G

A
x
G

K

G

A

V

I

I

V

I

C

T

P

P

G

G

V

I

R

T

C

I

K

G

S

D

H

N

S

V

I

V

L

I

S

G

V
x
A

G

G

S

S

V

V

A

V

T

T

K

K

D

D

L

I

E

P

Y

N

T

T

V

V

Y

A

Q
x
V

G

G

N

N

P

P

A

C

binding acetyl coenzyme *a: N84 (≠ G80), A104 (≠ S100), W138 (= W131), G140 (= G133), G141 (≠ A134), A159 (≠ V152), V174 (≠ Q167)

Sites not aligning to the query:

binding acetyl coenzyme *a: 182

5u2kA Crystal structure of galactoside o-acetyltransferase complex with coa (h3 space group)
30% identity, 75% coverage: 39:176/183 of query aligns to 44:183/190 of 5u2kA

query
sites
5u2kA

S

N

K

K

I

R

K

K

V

E

L

L

L

I

L

D

R

Q

I

L

F

F

G

Q

A

T

K

T

I

T

G

-

K

D

N

N

V

V

V

S

I

I

K

S

P

I

C

P

V

F

N

D

I

T

K

D

Y

Y

P

G

W

W

F

N

L

V

K

K

I

L

G

G

D

K

N

N

V

V

W

Y

I

V

G

N

E

T

N

N

V

C

W

Y

I

F

D
x
M

N

D

L

G

T

G

T

Q

V

I

E

T

I

I

G

G

N

D

N

N

V

V

C

F

I

I

S

G

Q

P

G

N

A

C

Y

G

I

F

F
x
Y

T

T

G
x
A

N

T

H
|
H

-

P

-

L

-

N

-

F

N

H

Y

H

K

R

T

N

Q

E

S

G

F

F

D

E

L

K

I

A

I

-

K

G

P

P

V

I

I

H

I

I

E

G

D

S

G

N

V

T

W

W

I

F

G

G

A

G

K

H

A

V

I

A

V

V

C
x
L

P

P

G

G

V

V

R

T

C

I

K

G

S

E

H

G

S

S

I

V

L

I

S

G

V

A

G

G

S

S

V

V

A

V

T
|
T

K
|
K

D

D

L

I

E

P

Y

H

T

S

V

L

Y

A

Q

V

G

G

N

N

P

P

A

C

V

K

P

V

K

V

R

R

K

K

binding coenzyme a: M90 (≠ D86), Y110 (≠ F106), A112 (≠ G108), H114 (= H110), L146 (≠ C139), T164 (= T157), K165 (= K158)

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
29% identity, 78% coverage: 29:170/183 of query aligns to 31:177/200 of 1krrA

query
sites
1krrA

L

L

F

M

F

Y

K

E

T

F

S

N

I

H

P

S

Y

H

P

P

S

S

K

E

I

V

-

E

-

K

K

R

V

E

L

S

L

L

L

I

R

K

I

E

F

M

G

F

A

A

K

T

I

V

G

G

K

E

N

N

V

A

V

W

I

V

K

E

P

P

C

P

V

V

N

Y

I

F

K

S

Y

Y

P

G

W

S

F

N

L

I

K

H

I

I

G

G

D

R

N

N

V

F

W

Y

I

A

G
x
N

E

F

N

N

V

L

W

T

I

I

D

V

N

D

L

D

T

Y

T

T

V

V

E

T

I

I

G

G

N

D

N

N

V

V

C

L

I
|
I

S
x
A

Q
x
P

G

N

A

V

Y

T

I

L

-

S

F

V

T
|
T

G

G

N
x
H

-

P

-

V

H

H

N

H

Y

E

K

L

T

R

Q

K

S

N

F

G

D

E

L

M

I

Y

I

S

K

F

P

P

V

I

I

T

I

I

E

G

D

N

G

N

V

V

W

W

I

I

G
|
G

A
x
S

K

H

A

V

I

V

V

I

C
x
N

P

P

G

G

V

V

R

T

C

I

K

G

S

D

H

N

S

S

I

V

L

I

S
x
G

V
x
A

G

G

S

S

V

I

A

V

T

T

K

K

D

D

L

I

E

P

Y

N

T

V

V

V

Y

A

Q
x
A

G

G

N

V

P
|
P

binding acetyl coenzyme *a: N84 (≠ G80), I103 (= I99), A104 (≠ S100), P105 (≠ Q101), T112 (= T107), H114 (≠ N109), G140 (= G133), S141 (≠ A134), N146 (≠ C139), G158 (≠ S151), A159 (≠ V152), A174 (≠ Q167), P177 (= P170)

Sites not aligning to the query:

binding acetyl coenzyme *a: 182

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
29% identity, 78% coverage: 29:170/183 of query aligns to 32:178/203 of P07464

query
sites
P07464

L

L

F

M

F

Y

K

E

T

F

S

N

I

H

P

S

Y

H

P

P

S

S

K

E

I

V

-

E

-

K

K

R

V

E

L

S

L

L

L

I

R

K

I

E

F

M

G

F

A

A

K

T

I

V

G

G

K

E

N

N

V

A

V

W

I

V

K

E

P

P

C

P

V

V

N

Y

I

F

K
x
S

Y

Y

P

G

W

S

F

N

L

I

K

H

I

I

G

G

D

R

N

N

V

F

W

Y

I

A

G
x
N

E

F

N

N

V

L

W

T

I

I

D

V

N

D

L
x
D

T

Y

T

T

V

V

E

T

I

I

G

G

N

D

N

N

V

V

C

L

I

I

S

A

Q

P

G

N

A

V

Y

T

I

L

-

S

F

V

T

T

G

G

N
x
H

-

P

-

V

H

H

N

H

Y

E

K

L

T

R

Q

K

S

N

F

G

D

E

L

M

I

Y

I

S

K

F

P

P

V

I

I

T

I

I

E

G

D

N

G

N

V

V

W

W

I

I

G

G

A
x
S

K

H

A

V

I

V

V

I

C

N

P

P

G

G

V

V

R

T

C

I

K

G

S

D

H

N

S

S

I

V

L

I

S

G

V
x
A

G

G

S

S

V

I

A

V

T
|
T

K
|
K

D

D

L

I

E

P

Y

N

T

V

V

V

Y

A

Q

A

G

G

N

V

P

P

S71 (≠ K66) binding
N85 (≠ G80) binding in other chain; binding in other chain
D93 (≠ L88) binding
H115 (≠ N109) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (≠ A134) binding in other chain
A160 (≠ V152) binding in other chain
TK 165:166 (= TK 157:158) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding
180 binding
183 binding in other chain

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
29% identity, 78% coverage: 29:170/183 of query aligns to 31:177/201 of 1krvA

query
sites
1krvA

L

L

F

M

F

Y

K

E

T

F

S

N

I

H

P

S

Y

H

P

P

S

S

K

E

I

V

-

E

-

K

K

R

V

E

L

S

L

L

L

I

R

K

I

E

F

M

G

F

A

A

K

T

I

V

G

G

K

E

N

N

V

A

V

W

I

V

K

E

P

P

C

P

V

V

N

Y

I

F

K
x
S

Y

Y

P

G

W

S

F

N

L

I

K

H

I

I

G

G

D

R

N

N

V

F

W
x
Y

I

A

G
x
N

E

F

N

N

V

L

W

T

I

I

D
x
V

N

D

L
x
D

T

Y

T

T

V

V

E

T

I

I

G

G

N

D

N

N

V

V

C

L

I

I

S
x
A

Q

P

G

N

A

V

Y

T

I

L

-
x
S

F

V

T
|
T

G

G

N

H

-

P

-

V

H

H

N

H

Y

E

K

L

T

R

Q

K

S

N

F

G

D

E

L
x
M

I

Y

I

S

K

F

P

P

V

I

I

T

I

I

E

G

D

N

G

N

V

V

W
|
W

I

I

G
|
G

A
x
S

K

H

A

V

I

V

V

I

C
x
N

P

P

G

G

V

V

R

T

C

I

K

G

S

D

H

N

S

S

I

V

L

I

S

G

V
x
A

G

G

S

S

V

I

A

V

T

T

K

K

D

D

L

I

E

P

Y

N

T

V

V

V

Y

A

Q

A

G

G

N

V

P
|
P

binding 4-nitrophenyl beta-D-galactopyranoside: S70 (≠ K66), Y82 (≠ W78), N84 (≠ G80), V90 (≠ D86), D92 (≠ L88), M126 (≠ L119)
binding coenzyme a: A104 (≠ S100), S110 (vs. gap), T112 (= T107), W138 (= W131), G140 (= G133), S141 (≠ A134), N146 (≠ C139), A159 (≠ V152), P177 (= P170)

Sites not aligning to the query:

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
29% identity, 78% coverage: 29:170/183 of query aligns to 31:177/201 of 1kruA

query
sites
1kruA

L

L

F

M

F

Y

K

E

T

F

S

N

I

H

P

S

Y

H

P

P

S

S

K

E

I

V

-

E

-

K

K

R

V

E

L

S

L

L

L

I

R

K

I

E

F

M

G

F

A

A

K

T

I

V

G

G

K

E

N

N

V

A

V
x
W

I

V

K

E

P

P

C

P

V

V

N

Y

I

F

K
x
S

Y

Y

P

G

W

S

F

N

L

I

K

H

I

I

G

G

D

R

N

N

V

F

W
x
Y

I

A

G
x
N

E

F

N

N

V

L

W

T

I

I

D
x
V

N

D

L
x
D

T

Y

T

T

V

V

E

T

I

I

G

G

N

D

N

N

V

V

C
x
L

I

I

S

A

Q

P

G

N

A

V

Y

T

I

L

-

S

F

V

T

T

G

G

N
x
H

-

P

-

V

H

H

N

H

Y

E

K

L

T

R

Q

K

S

N

F

G

D

E

L
x
M

I

Y

I

S

K

F

P

P

V

I

I

T

I

I

E

G

D

N

G

N

V

V

W
|
W

I

I

G
|
G

A
x
S

K

H

A

V

I

V

V

I

C

N

P

P

G

G

V

V

R

T

C

I

K

G

S

D

H

N

S

S

I

V

L

I

S
x
G

V
x
A

G

G

S

S

V

I

A

V

T

T

K

K

D

D

L

I

E

P

Y

N

T

V

V

V

Y

A

Q

A

G

G

N

V

P
|
P

binding coenzyme a: H114 (≠ N109), W138 (= W131), G140 (= G133), S141 (≠ A134), G158 (≠ S151), A159 (≠ V152), P177 (= P170)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: W62 (≠ V58), S70 (≠ K66), Y82 (≠ W78), Y82 (≠ W78), N84 (≠ G80), V90 (≠ D86), D92 (≠ L88), L102 (≠ C98), H114 (≠ N109), M126 (≠ L119)

Sites not aligning to the query:

3igjC Crystal structure of maltose o-acetyltransferase complexed with acetyl coenzyme a from bacillus anthracis
30% identity, 70% coverage: 43:171/183 of query aligns to 49:180/188 of 3igjC

query
sites
3igjC

V

T

L

L

L

N

R

Q

I

L

F

L

G

G

A

S

K

S

I

A

G

D

K

G

N

K

V

A

V

Q

I

I

K

N

P

P

C

D

V

F

N

R

I

C

K

D

Y

Y

P

G

W

Y

F

N

L

I

K

H

I

V

G

G

D

K

N

S

V

F

W
x
F

I

A

G

N

E

F

N

N

V

C

W

V

I

I

D

L

N

D

L

V

T

C

T

E

V

V

E

R

I

I

G

G

N

D

N

H

V

C

C

M

I

F

S
x
A

Q

P

G

G

A

V

Y

H

I

I

F
x
Y

T

T

G
x
A

N

T

H
|
H

N

P

Y

L

-

H

-

P

-

V

K

E

T

R

Q

N

S

S

F

G

D

K

L

E

I

Y

I

G

K

K

P

P

V

V

I

K

I

I

E

G

D

N

G

N

V

V

W

W

I

V

G
|
G

A

G

K

G

A

A

I

I

V

I

C
x
N

P

P

G

G

V

V

R

S

C

I

K

G

S

D

H

N

S

A

I

V

L

I

S
x
A

V
x
S

G

G

S

A

V

V

A

V

T
|
T

K

K

D

D

L

V

E

P

E

N

Y

N

T

V

V

V

Y

V

Q

G

G

G

N
|
N

P

P

A

A

binding acetyl coenzyme *a: F84 (≠ W78), A106 (≠ S100), Y112 (≠ F106), A114 (≠ G108), H116 (= H110), G142 (= G133), N148 (≠ C139), A160 (≠ S151), S161 (≠ V152), T166 (= T157), N178 (= N169)

8j40A Crystal structure of catb8 in complex with chloramphenicol (see paper)
26% identity, 52% coverage: 86:181/183 of query aligns to 51:167/209 of 8j40A

query
sites
8j40A

D

E

N

D

L

V

T

D

T

K

V

L

E

I

I

I

G

G

N

S

N

F

V

C

C

S

I

I

S

G

Q

S

G

G

A

A

-

S

Y

F

I

I

F

M

T

A

G

G

N

N

H

Q

N

G

Y

H

K

Q

-

H

-

D

-

W

-

A

-

S

-

F

-

P

-

F

-
x
Y

-

M

-

Q

-

E

-

P

-

A

-

F

T

S

Q

R

S

A

F

L

D

D

L

A

I

F

I

Q

K

R

P

A

-

G

-

D

V

T

I

V

I

I

E

G

D

N

G

D

V

V

W
|
W

I

I

G

G

A

S

K

E

A

A

I

M

V

I

C

M

P

P

G

G

V

I

R

K

C

I

K

G

S

D

H

G

S

A

I
x
V

L

I

S

G

V
x
S

G

R

S

S

V

L

A

V

T

T

K

K

D

D

L

V

E

E

E

P

Y

Y

T

A

V

I

Y

I

Q

G

G

G

N

N

P

P

A

A

V

K

P

Q

K

I

R

K

K

K

R

R

I

F

I

S

D

D

D

E

binding chloramphenicol: Y90 (vs. gap), W117 (= W131), V135 (≠ I149), S138 (≠ V152)

Sites not aligning to the query:

binding chloramphenicol: 7, 8, 9, 29, 31, 47, 50, 187, 190, 191, 202, 203, 206

2xatA Complex of the hexapeptide xenobiotic acetyltransferase with chloramphenicol and desulfo-coenzyme a (see paper)
28% identity, 52% coverage: 86:180/183 of query aligns to 50:165/208 of 2xatA

query
sites
2xatA

D

D

N

D

L

V

T

D

T

K

V

L

E

V

I

I

G

G

N

S

N

F

V

C

C

S

I

I

S

G

Q

S

G

G

A

A

-

A

Y

F

I

I

F

M

T

A

G

G

N

N

H

Q

N

G

Y

H

K

R

T

A

Q

E

-

W

-

A

-

S

-

T

-

F

S

P

F

F

D

H

L

F

I

M

I

H

K

E

P

E

-

P

-

A

-

F

-

A

-

G

-

A

-

V

-

N

-

G

-

Y

-

Q

-

P

-

A

-

G

-

D

V

T

I

L

I

I

E

G

D

H

G

E

V

V

W

W

I

I

G
|
G

A
x
T

K

E

A

A

I

M

V

F

C

M

P

P

G

G

V

V

R

R

C

V

K

G

S

H

H

G

S

A

I

I

L

I

S
x
G

V
x
S

G

R

S

A

V

L

A

V

T

T

K

G

D

D

L

V

E

E

E

P

Y

Y

T

A

V

I

Y

V

Q
x
G

G
|
G

N

N

P

P

A

A

V

R

P

T

K
x
I

R

R

K

K

R

R

I

F

I

S

D

D

binding desulfo-coenzyme a: G118 (= G133), T119 (≠ A134), G136 (≠ S151), S137 (≠ V152), G152 (≠ Q167), G153 (= G168), I159 (≠ K174)

Sites not aligning to the query:

binding chloramphenicol: 6, 7, 8, 28

3dhoA Structure of streptogramin acetyltransferase in complex with an inhibitor
36% identity, 32% coverage: 124:181/183 of query aligns to 114:171/203 of 3dhoA

query
sites
3dhoA

V

T

I

I

E

G

D

N

G

D

V

V

W

W

I

I

G

G

A

K

K

D

A

V

I

V

V

I

C

M

P

P

G

G

V

V

R

K

C

I

K

G

S

D

H

G

S

A

I
|
I

L

V

S
x
A

V

A

G

N

S

S

V

V

A

V

T
x
V

K

K

D

D

L

I

E

A

E

P

Y

Y

T

M

V
x
L

Y

A

Q
x
G

G

G

N

N

P
|
P

A

A

V

N

P

E

K
x
I

R

K

K

Q

R

R

I

F

I

D

D

Q

D

D

binding 6-bromo-N'-[(1Z)-(3,5-dichloro-2-hydroxyphenyl)methylidene]-2-methylquinoline-4-carbohydrazide: I139 (= I149), A141 (≠ S151), V147 (≠ T157), L155 (≠ V165), G157 (≠ Q167), P160 (= P170), I164 (≠ K174)

Sites not aligning to the query:

binding 6-bromo-N'-[(1Z)-(3,5-dichloro-2-hydroxyphenyl)methylidene]-2-methylquinoline-4-carbohydrazide: 84

1khrA Crystal structure of vat(d) in complex with virginiamycin and coenzyme a (see paper)
36% identity, 32% coverage: 124:181/183 of query aligns to 114:171/206 of 1khrA

query
sites
1khrA

V

T

I

I

E

G

D

N

G

D

V

V

W
|
W

I

I

G

G

A

K

K

D

A

V

I

V

V

I

C

M

P

P

G

G

V

V

R

K

C

I

K

G

S

D

H

G

S

A

I

I

L

V

S
x
A

V
x
A

G

N

S

S

V

V

A

V

T
x
V

K

K

D

D

L

I

E

A

E

P

Y

Y

T

M

V
x
L

Y

A

Q
x
G

G

G

N

N

P
|
P

A

A

V

N

P

E

K
x
I

R

K

K

Q

R

R

I

F

I

D

D

Q

D

D

binding coenzyme a: W121 (= W131), A141 (≠ S151), A142 (≠ V152), V147 (≠ T157), L155 (≠ V165), G157 (≠ Q167), P160 (= P170), I164 (≠ K174)

Sites not aligning to the query:

binding coenzyme a: 82, 84
binding virginiamycin m1: 37, 39, 54, 80, 81, 82, 93

P50870 Streptogramin A acetyltransferase; Virginiamycin acetyltransferase D; Vat(D); EC 2.3.1.- from Enterococcus faecium (Streptococcus faecium) (see paper)
36% identity, 32% coverage: 124:181/183 of query aligns to 114:171/209 of P50870

query
sites
P50870

V

T

I

I

E

G

D

N

G

D

V

V

W

W

I

I

G

G

A

K

K

D

A

V

I

V

V

I

C

M

P

P

G

G

V

V

R

K

C

I

K

G

S

D

H

G

S

A

I

I

L

V

S

A

V

A

G

N

S

S

V

V

A

V

T

V

K

K

D

D

L

I

E

A

E

P

Y

Y

T

M

V

L

Y

A

Q

G

G

G

N

N

P

P

A

A

V

N

P

E

K

I

R

K

K

Q

R

R

I

F

I

D

D

Q

D

D

Sites not aligning to the query:

82 H→A: 105-fold decrease in activity.

1mrlA Crystal structure of streptogramin a acetyltransferase with dalfopristin (see paper)
36% identity, 32% coverage: 124:181/183 of query aligns to 114:171/204 of 1mrlA

query
sites
1mrlA

V

T

I

I

E

G

D

N

G

D

V

V

W

W

I

I

G

G

A

K

K

D

A

V

I

V

V

I

C

M

P

P

G

G

V

V

R

K

C

I

K

G

S

D

H

G

S

A

I

I

L

V

S

A

V

A

G

N

S

S

V

V

A

V

T

V

K

K

D

D

L

I

E

A

E

P

Y

Y

T

M

V

L

Y

A

Q

G

G

G

N

N

P

P

A

A

V

N

P

E

K

I

R

K

K

Q

R

R

I

F

I

D

D

Q

D

D

Sites not aligning to the query:

binding 5-(2-diethylamino-ethanesulfonyl)-21-hydroxy-10-isopropyl-11,19-dimethyl-9,26-dioxa-3,15,28-triaza-tricyclo[23.2.1.00,255]octacosa-1(27),12,17,19,25(28)-pentaene-2,8,14,23-tetraone: 37, 39, 54, 56, 81, 82, 93, 102, 108

1kk4A Crystal structure of vat(d) in complex with acetyl-coa (see paper)
36% identity, 32% coverage: 124:181/183 of query aligns to 114:171/205 of 1kk4A

query
sites
1kk4A

V

T

I

I

E

G

D

N

G

D

V

V

W
|
W

I

I

G
|
G

A
x
K

K

D

A

V

I

V

V

I

C

M

P

P

G

G

V

V

R

K

C

I

K

G

S

D

H

G

S

A

I

I

L

V

S
x
A

V
x
A

G

N

S

S

V

V

A

V

T
x
V

K
|
K

D

D

L

I

E

A

E

P

Y

Y

T

M

V
x
L

Y

A

Q
x
G

G
|
G

N

N

P
|
P

A

A

V

N

P

E

K
x
I

R

K

K

Q

R

R

I

F

I

D

D

Q

D

D

binding acetyl coenzyme *a: W121 (= W131), G123 (= G133), K124 (≠ A134), A141 (≠ S151), A142 (≠ V152), V147 (≠ T157), K148 (= K158), L155 (≠ V165), G157 (≠ Q167), G158 (= G168), P160 (= P170), I164 (≠ K174)

Sites not aligning to the query:

binding acetyl coenzyme *a: 69, 70, 111

4hurA Crystal structure of streptogramin group a antibiotic acetyltransferase vata from staphylococcus aureus in complex with acetyl coenzyme a (see paper)
33% identity, 59% coverage: 73:180/183 of query aligns to 56:169/211 of 4hurA

query
sites
4hurA

I

I

G

G

D

D

N

K

V

L

W

I

I

I

G

G

E

R

N

F

V

C

W
x
S

I
|
I

D
x
G

N

P

L

G

T

T

-

F

V

I

E

M

I

N

G

G

N
x
A

N
|
N

V

H

C

R

I

M

S

D

Q

G

G

S

A

T

Y

Y

I

P

F

F

-

H

-

L

-

F

-

R

T

M

G

G

N

W

H

E

N

K

Y

Y

K

M

T

P

Q

S

S

L

F

K

D

D

L

L

I

P

I

L

K
|
K

P

G

V

D

I

I

-

E

I

I

E

G

D

N

G

D

V

V

W
|
W

I
|
I

G
|
G

A
x
R

K

D

A

V

I

T

V

I

C
x
M

P

P

G

G

V

V

R

K

C

I

K

G

S

D

H

G

S

A

I

I

L

I

S
x
A

V
x
A

G

E

S

A

V

V

A

V

T
|
T

K

K

D

N

L

V

E

A

E

P

Y

Y

T

S

V

I

Y

V

Q
x
G

G

G

N

N

P
|
P

A

L

V

K

P

F

K
x
I

R
|
R

K

K

R

R

I

F

I

S

D

D

binding acetyl coenzyme *a: S67 (≠ W84), I68 (= I85), G69 (≠ D86), A79 (≠ N95), N80 (= N96), K110 (= K122), W120 (= W131), I121 (= I132), G122 (= G133), R123 (≠ A134), M128 (≠ C139), A140 (≠ S151), A141 (≠ V152), T146 (= T157), G156 (≠ Q167), P159 (= P170), I163 (≠ K174), R164 (= R175)

Sites not aligning to the query:

binding acetyl coenzyme *a: 51

6u9cA The 2.2 a crystal structure of the type b chloramphenicol acetyltransferase from vibrio cholerae in the complex with acetyl coa
28% identity, 52% coverage: 86:180/183 of query aligns to 51:165/206 of 6u9cA

query
sites
6u9cA

D

D

N

D

L

V

T

D

T

K

V

L

E

V

I

I

G

G

N

S

N

F

V

C

C

S

I

I

S

G

Q

S

G

G

A

A

-

V

Y

F

I

M

F

M

T

A

G

G

N

N

H

Q

N

G

Y

H

K

R

T

S

-

D

-

W

-

I

Q

S

S

T

F

F

D

P

L

F

I

F

I

Y

K

Q

P

D

-

N

-

D

-

N

-

F

-

A

-

D

-

A

-

R

-

D

-

G

-

F

-

T

-

R

-

S

-

G

-

D

V

T

I

I

E

G

D

H

G

D

V

V

W
|
W

I

I

G

G

A

T

K

E

A

A

I

M

V

I

C

M

P

P

G

G

V

V

R

K

C

I

K

G

S

H

H

G

S

A

I

I

L

I

S
x
A

V

S

G

R

S

S

V

V

A

V

T

T

K

K

D

D

L

V

E

A

E

P

Y

Y

T

E

V

V

Y

V

Q

G

G

S

N

N

P

P

A

A

V

K

P

H

K
x
I

R

K

K

F

R

R

I

F

I

S

D

D

binding acetyl coenzyme *a: W116 (= W131), A136 (≠ S151), I159 (≠ K174)

6x3jA Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f0224 (46) (see paper)
33% identity, 59% coverage: 73:180/183 of query aligns to 56:169/206 of 6x3jA

query
sites
6x3jA

I

I

G

G

D

D

N

K

V

L

W

I

I

I

G

G

E

R

N

F

V

C

W

S

I

I

D
x
G

N

P

L

G

T

T

-

F

V

I

E

M

I

N

G

G

N
x
A

N

N

V
x
H

C

R

I

M

S

D

Q

G

G

S

A

T

Y

Y

I

P

F

F

-
x
H

-
x
L

-

F

-

R

T

M

G

G

N

W

H

E

N

K

Y

Y

K
x
M

T
x
P

Q

S

S

L

F

K

D

D

L
|
L

I

P

I

L

K

K

P

G

V

D

I

I

-

E

I

I

E

G

D

N

G

D

V

V

W
|
W

I

I

G
|
G

A

R

K

D

A

V

I

T

V

I

C

M

P

P

G

G

V

V

R

K

C

I

K

G

S

D

H

G

S

A

I

I

L

I

S

A

V

A

G

E

S

A

V

V

A

V

T

T

K

K

D

N

L

V

E

A

E

P

Y

Y

T

S

V

I

Y

V

Q

G

G

G

N

N

P

P

A

L

V

K

P

F

K

I

R

R

K

K

R

R

I

F

I

S

D

D

binding (2R)-2-[(3S,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-4,12-dimethyl-1,7,22-trioxo-4,7,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,3H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosin-3-yl]propyl isoquinolin-3-ylcarbamate: H91 (vs. gap), L92 (vs. gap), M101 (≠ K113), P102 (≠ T114), L107 (= L119)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: G69 (≠ D86), A79 (≠ N95), H81 (≠ V97), W120 (= W131), G122 (= G133)

Sites not aligning to the query:

binding (2R)-2-[(3S,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-4,12-dimethyl-1,7,22-trioxo-4,7,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,3H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosin-3-yl]propyl isoquinolin-3-ylcarbamate: 51, 53, 55

Query Sequence

>WP_013452079.1 NCBI__GCF_000183405.1:WP_013452079.1
MVDLSKYDNSWYDPGNKIKILIWYFINLLFFKTSIPYPSKIKVLLLRIFGAKIGKNVVIK
PCVNIKYPWFLKIGDNVWIGENVWIDNLTTVEIGNNVCISQGAYIFTGNHNYKTQSFDLI
IKPVIIEDGVWIGAKAIVCPGVRCKSHSILSVGSVATKDLEEYTVYQGNPAVPKRKRIID
DGT

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory