SitesBLAST
Comparing WP_013458941.1 NCBI__GCF_000183725.1:WP_013458941.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
1kjiA Crystal structure of glycinamide ribonucleotide transformylase in complex with mg-amppcp (see paper)
53% identity, 99% coverage: 2:385/387 of query aligns to 2:386/389 of 1kjiA
- active site: E114 (≠ K114), K154 (= K154), S159 (= S159), G161 (= G161), E264 (= E265), E276 (= E277), D283 (= D284), T284 (= T285), R360 (= R360)
- binding phosphomethylphosphonic acid adenylate ester: R113 (= R113), I152 (≠ V152), K154 (= K154), S159 (= S159), S160 (= S160), G161 (= G161), Q164 (= Q164), E192 (= E193), V195 (≠ I196), E200 (= E201), Q222 (= Q223), E264 (= E265), F266 (= F267), E276 (= E277)
- binding magnesium ion: E264 (= E265), E276 (= E277)
1ez1A Structure of escherichia coli purt-encoded glycinamide ribonucleotide transformylase complexed with mg, amppnp, and gar (see paper)
53% identity, 99% coverage: 2:385/387 of query aligns to 2:386/389 of 1ez1A
- active site: E114 (≠ K114), K154 (= K154), S159 (= S159), G161 (= G161), E264 (= E265), E276 (= E277), D283 (= D284), T284 (= T285), R360 (= R360)
- binding phosphoaminophosphonic acid-adenylate ester: R113 (= R113), I152 (≠ V152), K154 (= K154), S159 (= S159), S160 (= S160), G161 (= G161), E192 (= E193), V194 (≠ F195), V195 (≠ I196), F197 (= F198), E200 (= E201), Q222 (= Q223), E264 (= E265), F266 (= F267), E276 (= E277)
- binding glycinamide ribonucleotide: G20 (= G20), E21 (= E21), L22 (= L22), E81 (= E81), I82 (= I82), S160 (= S160), D283 (= D284), K352 (= K352), R359 (= R359), R360 (= R360)
- binding magnesium ion: E264 (= E265), E276 (= E277)
1eyzA Structure of escherichia coli purt-encoded glycinamide ribonucleotide transformylase complexed with mg and amppnp (see paper)
53% identity, 99% coverage: 2:385/387 of query aligns to 2:386/389 of 1eyzA
- active site: E114 (≠ K114), K154 (= K154), S159 (= S159), G161 (= G161), E264 (= E265), E276 (= E277), D283 (= D284), T284 (= T285), R360 (= R360)
- binding phosphoaminophosphonic acid-adenylate ester: R113 (= R113), I152 (≠ V152), K154 (= K154), S159 (= S159), S160 (= S160), G161 (= G161), Q164 (= Q164), E192 (= E193), V195 (≠ I196), F197 (= F198), E200 (= E201), E264 (= E265), F266 (= F267), E276 (= E277)
- binding magnesium ion: E264 (= E265), E276 (= E277)
P33221 Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; GAR transformylase T; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 6.3.1.21 from Escherichia coli (strain K12) (see 5 papers)
53% identity, 99% coverage: 2:385/387 of query aligns to 3:389/392 of P33221
- EL 22:23 (= EL 21:22) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- E82 (= E81) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- R114 (= R113) binding ATP
- K155 (= K154) binding ATP
- SSGKGQ 160:165 (≠ SSGHGQ 159:164) binding ATP
- G162 (= G161) mutation to I: Strong decrease in the reaction rate for the conversion of formate to FGAR and in the affinity for formate. 3- and 2-fold decrease in the affinity for ATP and GAR, respectively.
- K179 (≠ E178) modified: N6-acetyllysine
- EGVV 195:198 (≠ EEFI 193:196) binding ATP
- E203 (= E201) binding ATP
- E267 (= E265) binding Mg(2+)
- E279 (= E277) binding Mg(2+)
- D286 (= D284) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- K355 (= K352) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- RR 362:363 (= RR 359:360) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
1kjjA Crystal structure of glycniamide ribonucleotide transformylase in complex with mg-atp-gamma-s (see paper)
53% identity, 99% coverage: 2:385/387 of query aligns to 2:383/386 of 1kjjA
- active site: E114 (≠ K114), K154 (= K154), S159 (= S159), G161 (= G161), E261 (= E265), E273 (= E277), D280 (= D284), T281 (= T285), R357 (= R360)
- binding phosphothiophosphoric acid-adenylate ester: R113 (= R113), I152 (≠ V152), K154 (= K154), S159 (= S159), S160 (= S160), G161 (= G161), Q164 (= Q164), E189 (= E193), V192 (≠ I196), E197 (= E201), Q219 (= Q223), E261 (= E265), F263 (= F267), E273 (= E277)
- binding magnesium ion: E261 (= E265), E273 (= E277)
1kj8A Crystal structure of purt-encoded glycinamide ribonucleotide transformylase in complex with mg-atp and gar (see paper)
53% identity, 99% coverage: 2:385/387 of query aligns to 2:383/386 of 1kj8A
- active site: E114 (≠ K114), K154 (= K154), S159 (= S159), G161 (= G161), E261 (= E265), E273 (= E277), D280 (= D284), T281 (= T285), R357 (= R360)
- binding adenosine-5'-triphosphate: R113 (= R113), I152 (≠ V152), K154 (= K154), S159 (= S159), S160 (= S160), G161 (= G161), Q164 (= Q164), E189 (= E193), V192 (≠ I196), F194 (= F198), E197 (= E201), Q219 (= Q223), G222 (= G226), E261 (= E265), F263 (= F267), E273 (= E277)
- binding glycinamide ribonucleotide: G20 (= G20), E21 (= E21), L22 (= L22), E81 (= E81), I82 (= I82), S160 (= S160), D280 (= D284), K349 (= K352), R356 (= R359)
- binding magnesium ion: E261 (= E265), E273 (= E277)
1kjqA Crystal structure of glycinamide ribonucleotide transformylase in complex with mg-adp (see paper)
52% identity, 99% coverage: 2:385/387 of query aligns to 2:385/388 of 1kjqA
- active site: E114 (≠ K114), K154 (= K154), E263 (= E265), E275 (= E277), D282 (= D284), T283 (= T285), R359 (= R360)
- binding adenosine-5'-diphosphate: R113 (= R113), I152 (≠ V152), K154 (= K154), E191 (= E193), V193 (≠ F195), V194 (≠ I196), F196 (= F198), E199 (= E201), Q221 (= Q223), F265 (= F267), E275 (= E277)
- binding magnesium ion: E263 (= E265), E275 (= E277)
O58056 Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 6.3.1.21 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
49% identity, 97% coverage: 9:385/387 of query aligns to 14:409/430 of O58056
2dwcB Crystal structure of probable phosphoribosylglycinamide formyl transferase from pyrococcus horikoshii ot3 complexed with adp
47% identity, 97% coverage: 9:385/387 of query aligns to 16:398/409 of 2dwcB
- active site: E265 (= E265), E277 (= E277), D284 (= D284), T285 (= T285), R372 (= R360)
- binding adenosine-5'-diphosphate: R120 (= R113), H159 (≠ V152), K161 (= K154), H190 (≠ F195), I191 (= I196), F193 (= F198), E196 (= E201), F267 (= F267), E277 (= E277)
3q2oB Crystal structure of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
27% identity, 93% coverage: 21:380/387 of query aligns to 18:369/377 of 3q2oB
3v4sB Crystal structure of adp-atp complex of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
27% identity, 93% coverage: 21:380/387 of query aligns to 19:370/381 of 3v4sB
- binding adenosine-5'-triphosphate: R108 (= R113), K148 (= K154), Y154 (vs. gap), D155 (≠ S160), G156 (= G161), Q159 (= Q164), E183 (= E193), W185 (≠ F195), V186 (≠ I196), F188 (= F198), E191 (= E201), H214 (≠ Q223), N217 (≠ G226), E256 (= E265), F258 (= F267), E269 (= E277)
- binding carbonate ion: R273 (= R281), H275 (= H283), N276 (≠ D284)
- binding magnesium ion: T105 (= T110), E111 (≠ R117), E256 (= E265), E269 (= E277), L270 (≠ V278)
3v4sA Crystal structure of adp-atp complex of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
27% identity, 93% coverage: 21:380/387 of query aligns to 17:368/380 of 3v4sA
- binding adenosine-5'-diphosphate: R106 (= R113), K146 (= K154), Y152 (vs. gap), G154 (= G161), Q157 (= Q164), W183 (≠ F195), V184 (≠ I196), E189 (= E201), N215 (≠ G226), F256 (= F267), N266 (≠ S276), E267 (= E277)
- binding carbonate ion: R271 (= R281), H273 (= H283), N274 (≠ D284)
3r5hA Crystal structure of adp-air complex of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
27% identity, 93% coverage: 21:380/387 of query aligns to 18:369/383 of 3r5hA
- binding adenosine-5'-diphosphate: R107 (= R113), K147 (= K154), Q158 (= Q164), W184 (≠ F195), V185 (≠ I196), F187 (= F198), E190 (= E201), N216 (≠ G226), F257 (= F267), N267 (≠ S276), E268 (= E277)
- binding 5-aminoimidazole ribonucleotide: Q18 (≠ E21), L19 (= L22), E76 (= E81), Y153 (vs. gap), R272 (= R281), K340 (= K352), R347 (= R359)
Sites not aligning to the query:
4dlkA Crystal structure of atp-ca++ complex of purk: n5- carboxyaminoimidazole ribonucleotide synthetase (see paper)
27% identity, 93% coverage: 21:380/387 of query aligns to 18:369/380 of 4dlkA
- active site: Y153 (vs. gap), G155 (= G161), E255 (= E265), E268 (= E277), N275 (≠ D284), S276 (≠ T285), K348 (≠ R360)
- binding adenosine-5'-triphosphate: E76 (= E81), F77 (≠ I82), R107 (= R113), K147 (= K154), Y153 (vs. gap), D154 (≠ S160), G155 (= G161), Q158 (= Q164), W184 (≠ F195), V185 (≠ I196), F187 (= F198), E190 (= E201), E255 (= E265), F257 (= F267), N267 (≠ S276), E268 (= E277), R272 (= R281), H274 (= H283), N275 (≠ D284), K340 (= K352), R347 (= R359), K348 (≠ R360)
- binding calcium ion: E255 (= E265), E268 (= E277)
- binding phosphate ion: Q47 (≠ L50), A49 (= A52)
4mamA The crystal structure of phosphoribosylaminoimidazole carboxylase atpase subunit of francisella tularensis subsp. Tularensis schu s4 in complex with an adp analog, amp-cp
27% identity, 78% coverage: 13:313/387 of query aligns to 2:295/373 of 4mamA
- active site: Y144 (≠ S159), G146 (= G161), E247 (= E265), E259 (= E277), N266 (≠ D284), S267 (≠ T285)
- binding phosphomethylphosphonic acid adenosyl ester: R98 (= R113), I136 (≠ V152), K138 (= K154), Y144 (≠ S159), G146 (= G161), Q149 (= Q164), E175 (= E193), F177 (= F195), V178 (≠ I196), F180 (= F198), E183 (= E201), H206 (≠ Q223), F249 (= F267), E259 (= E277)
Sites not aligning to the query:
4ma0A The crystal structure of phosphoribosylaminoimidazole carboxylase atpase subunit of francisella tularensis subsp. Tularensis schu s4 in complex with partially hydrolysed atp
27% identity, 78% coverage: 13:313/387 of query aligns to 2:295/366 of 4ma0A
- active site: Y144 (≠ S159), G146 (= G161), E247 (= E265), E259 (= E277), N266 (≠ D284), S267 (≠ T285)
- binding adenosine monophosphate: I136 (≠ V152), K138 (= K154), E175 (= E193), A176 (≠ E194), F177 (= F195), V178 (≠ I196), E183 (= E201), H206 (≠ Q223), F249 (= F267), E259 (= E277)
Sites not aligning to the query:
5jqwA The crystal structure of phosphoribosylaminoimidazole carboxylase atpase subunit of francisella tularensis subsp. Tularensis schu s4 in complex with adp
27% identity, 78% coverage: 13:313/387 of query aligns to 2:295/365 of 5jqwA
- active site: Y144 (≠ S159), G146 (= G161), E247 (= E265), E259 (= E277), N266 (≠ D284), S267 (≠ T285)
- binding adenosine-5'-diphosphate: R98 (= R113), K138 (= K154), G143 (≠ S158), Y144 (≠ S159), D145 (≠ S160), G146 (= G161), V178 (≠ I196), E183 (= E201), H206 (≠ Q223), F249 (= F267), E259 (= E277)
Sites not aligning to the query:
3ax6A Crystal structure of n5-carboxyaminoimidazole ribonucleotide synthetase from thermotoga maritima
24% identity, 93% coverage: 26:386/387 of query aligns to 16:360/360 of 3ax6A
- active site: E231 (= E265), E244 (= E277), N251 (≠ D284), S252 (≠ T285), K330 (≠ R360)
- binding adenosine-5'-diphosphate: K101 (≠ R113), V136 (= V152), K138 (= K154), E164 (= E193), F166 (= F195), V167 (≠ I196), E172 (= E201), F233 (= F267), N243 (≠ S276)
4ma5A The crystal structure of phosphoribosylaminoimidazole carboxylase atpase subunit of francisella tularensis subsp. Tularensis schu s4 in complex with an atp analog, amp-pnp.
26% identity, 78% coverage: 13:313/387 of query aligns to 2:293/363 of 4ma5A
- active site: Y144 (≠ S159), G146 (= G161), E247 (= E265), E257 (= E277), N264 (≠ D284), S265 (≠ T285)
- binding phosphoaminophosphonic acid-adenylate ester: I136 (≠ V152), K138 (= K154), D145 (≠ S160), G146 (= G161), F177 (= F195), V178 (≠ I196), E183 (= E201), H206 (≠ Q223), E247 (= E265), F249 (= F267), N256 (≠ S276), E257 (= E277), H263 (= H283)
Sites not aligning to the query:
3aw8A Crystal structure of n5-carboxyaminoimidazole ribonucleotide synthetase from thermus thermophilus hb8
32% identity, 60% coverage: 81:313/387 of query aligns to 66:288/360 of 3aw8A
- active site: E240 (= E265), E252 (= E277), N259 (≠ D284), S260 (≠ T285)
- binding adenosine monophosphate: L135 (≠ V152), K137 (= K154), Q142 (= Q164), F168 (= F195), V169 (≠ I196), E174 (= E201), H197 (≠ K224), F242 (= F267), E252 (= E277)
Sites not aligning to the query:
Query Sequence
>WP_013458941.1 NCBI__GCF_000183725.1:WP_013458941.1
MLFSAPLKNNSKKIMLLGSGELGKEVAIEAQRLGIEVIAVDRYNHAPAHLVANRSHVVNM
QDKEAVLKLIRKEKPDYILPEIEAISIDALFAAEAEGFCVIPNAEAVNKTMNRKNIRRFA
AEDLGLKTSRYHFVSTYEDMCASAEDVGFPCVIKPVMSSSGHGQSIARTREDLAASWEIA
KEARGDASELIVEEFIRFDYEITLLTARNGVETVFCEPIGHIQKDGDYIYSWQPMEMSKK
ALKRSQKIAKAITDGLGGKGLFGVELFVAGDEVYFSEVSPRPHDTGMVTLITQSQSEFAL
HVRAVLGLPLGFTFYGDGASAAFKSVVESTEPVVDVADEVFDDNSFVRVFGKPEAHVGRR
MAVVLVFDKVNKAMKKAKKLIKKIKDA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory