SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013459152.1 NCBI__GCF_000183725.1:WP_013459152.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P36204 Bifunctional PGK/TIM; EC 2.7.2.3; EC 5.3.1.1 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
50% identity, 99% coverage: 1:391/396 of query aligns to 1:391/654 of P36204

query
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P36204

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R36 (= R37) binding substrate
R118 (= R119) binding substrate
R151 (= R152) binding substrate

1vpeA Crystallographic analysis of phosphoglycerate kinase from the hyperthermophilic bacterium thermotoga maritima (see paper)
51% identity, 98% coverage: 4:391/396 of query aligns to 3:390/398 of 1vpeA

query
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1vpeA

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active site: R35 (= R37), K196 (= K201), G353 (= G354), G376 (= G377)
binding phosphoaminophosphonic acid-adenylate ester: G194 (= G199), A195 (≠ S200), K196 (= K201), K200 (= K205), G218 (= G223), A219 (≠ G224), N316 (= N320), P318 (= P322), G320 (= G324), V321 (= V325), E323 (= E327), G352 (= G353), G353 (= G354), D354 (= D355), S355 (≠ T356)

1phpA Structure of the adp complex of the 3-phosphoglycerate kinase from bacillus stearothermophilus at 1.65 angstroms (see paper)
51% identity, 99% coverage: 1:394/396 of query aligns to 1:391/394 of 1phpA

query
sites
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active site: R36 (= R37), K197 (= K201), G351 (= G354), G374 (= G377)
binding adenosine-5'-diphosphate: G195 (= G199), K201 (= K205), G219 (= G223), G220 (= G224), L237 (= L241), N316 (= N320), P318 (= P322), G320 (= G324), V321 (= V325), E323 (= E327), G350 (= G353), D352 (= D355), S353 (≠ T356)

P18912 Phosphoglycerate kinase; EC 2.7.2.3 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
51% identity, 99% coverage: 1:394/396 of query aligns to 1:391/394 of P18912

query
sites
P18912

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R

L

L

A

D

P

A

I

V

A

A

R

K

R

R

L

L

H

G

Q

E

L

L

K

E

I

R

D

P

V

V

Q

A

V

K

A

T

D

N

D

E

V

A

V

V

G

G

E

D

S

E

A

V

L

K

K

A

L

A

A

V

H

D

E

R

L

L

K

N

N

E

G

G

E

D

V

V

L

L

E

E

N

N

L

V

R

R

F

F

E

Y

K

P

G

G

E

E

T

E

K

K

N

N

D

D

P

P

D

E

L

L

S

A

A

K

A

A

L

F

A

A

S

E

M

L

A

A

E

D

V

L

Y

Y

I

V

N

N

D

D

A

A

F

F

G

G

V

A

S

A

H

H

R

R

A

A

H

H

A

A

S

S

V

T

E

E

G

G

I

I

T

-

A

A

H

H

F

Y

D

L

N

P

A

A

H

-

K

-

A

V

A

A

G

G

F

F

L

L

L

M

Q

E

K

K

E

E

I

L

Q

E

F

V

F

L

G

G

T

K

L

A

L

L

E

S

H

N

P

P

V

D

R

R

P

P

F

F

A

T

A

A

I

I

V

I

G

G

S

A

K

K

V

V

S

K

G

D

K
|
K

L

I

E

G

A

V

L

I

I

D

N

N

L

L

P

E

K

K

V

V

D

D

K

N

M

L

L

I

I

I

G

G

M

L

A

A

F

Y

T

T

F

F

L

V

K

K

A

A

L

L

G

G

H

H

D

D

V

V

G

G

N

K

S

S

L

L

V

L

E

E

D

E

D

D

L

K

I

I

P

E

E

L

A

A

L

K

K

S

I

F

M

M

E

E

E

K

A

A

K

K

R

E

L

K

G

G

V

V

K

R

F

F

Y

Y

L

M

P

P

V

V

D

D

V

V

A

V

A

A

E

D

K

R

F

F

A

A

P

N

D

D

S

A

A

N

S

T

R

K

L

V

C

V

S

P

V

I

Q

D

E

A

I

I

P

P

S

A

G

D

W

W

M

S

G

A

L

L

D

D

I

I

G

G

P

P

A

K

T

T

V

R

R

E

L

L

Y

Y

R

R

Q

D

V

V

L

I

G

R

D

E

V

S

Q

K

T

L

I

V

L

V

W

W

N
|
N

G

G

P

P

M

M

G

G

V

V

Y

F

E
|
E

M

M

D

D

R

A

Y

F

A

A

R

H

G

G

S

T

N

K

-

A

-

I

-

A

K

E

I

A

A

L

H

A

F

E

V

A

A

L

D

D

S

T

Y

Y

A

-

T

-

S

S

V

V

V

I

G

G

G
|
G

D
|
D

T
x
S

A

A

D

A

L

A

V

V

Q

E

R

K

V

F

G

G

L

L

D

A

E

D

E

K

I

M

T

D

F

H

I

I

S

S

T

T

G

G

A

A

S

S

L

L

E

E

L

F

L

M

E

E

G

G

K

K

I

Q

L

L

P

P

G

G

V

V

K

V

P

A

L

L

K201 (= K205) binding ATP
N316 (= N320) binding ATP
E323 (= E327) binding ATP
GGDS 350:353 (≠ GGDT 353:356) binding ATP

P40924 Phosphoglycerate kinase; EC 2.7.2.3 from Bacillus subtilis (strain 168) (see paper)
49% identity, 99% coverage: 1:394/396 of query aligns to 1:391/394 of P40924

query
sites
P40924

M

M

Q

N

L

K

G

T

I

L

K

K

E

D

C

I

D

D

I

V

H

K

G

G

K

K

K

V

V

V

F

F

I

C

R

R

C

V

D

D

F

F

N

N

V

V

P

P

M

M

D

K

D

D

Y

-

G

G

N

E

I

V

T

T

D

D

R

T

R

R

I

I

R

R

S

A

A

A

L

L

A

P

T

T

I

I

N

K

Y

H

C

L

L

A

D

D

Q

Q

K

G

C

A

A

K

I

V

I

L

L

L

A

A

S

S

H

H

F

L

G

G

R

R

P

P

K

K

G

G

E

E

P

V

D

V

E

E

K

E

Y

L

S

R

L

L

A

T

P

P

I

V

A

A

R

A

R

R

L

L

H

G

Q

E

L

L

K

G

I

K

D

E

V

V

Q

K

V

K

A

A

D

D

D

E

V

A

V

Y

G

G

E

D

S

A

A

V

L

K

K

A

L

Q

A

I

H

S

E

E

L

M

K

K

N

D

G

G

E

D

V

V

L

L

L

V

L

L

E

E

N

N

L

V

R

R

F

F

E

Y

K

P

G

G

E

E

T

E

K

K

N

N

D

D

P

P

D

E

L

L

S

A

A

K

A

A

L

F

A

A

S

E

M

L

A

A

E

D

V

V

Y

Y

I

V

N

N

D

D

A

A

F

F

G

G

V

A

S

A

H

H

R

R

A

A

H

H

A

A

S

S

V

T

E

A

G

G

I

I

T

A

A

E

H

H

F

L

D

-

N

-

A

-

H

P

K

A

A

V

A

A

G

G

F

F

L

L

L

M

Q

E

K

K

E

E

I

L

Q

D

F

V

F

L

G

G

T

K

L

A

L

V

E
x
S

H

N

P

P

V

D

R

R

P

P

F

F

A

T

A

A

I

I

V

I

G

G

S

A

K

K

V

V

S

K

G

D

K

K

L

I

E

G

A

V

L

I

I

E

N

S

L

L

P

D

K

K

V

V

D

D

K

N

M

L

L

I

I

I

G

G

M

L

A

A

F

Y

T

T

F

F

L

V

K

K

A

A

L

L

G

G

H

Y

D

E

V

V

G

G

N

K

S

S

L

L

V

L

E

E

D

E

D

D

L

K

I

I

P

E

E

L

A

A

L

K

K

S

I

F

M

M

E

D

E

R

A

A

K

K

R

E

L

K

G

G

V

V

K

N

F

F

Y

Y

L

M

P

P

V

E

D

D

V

V

V

L

A

V

A

A

E

D

K

D

F

F

A

S

P

N

D

D

S

A

A

N

S

V

R

K

L

I

C

V

S

P

V

I

Q

S

E

E

I

I

P

P

S

S

G

D

W

L

M

E

G

A

L

I

D

D

I

I

G

G

P

T

A

K

T
|
T

V

R

R

E

L

T

Y

Y

R

A

Q

D

V

V

L

I

G

K

D

N

V

S

Q

K

T

L

I

V

L

V

W

W

N

N

G

G

P

P

M

M

G

G

V

V

Y

F

E

E

M

I

D

D

R

L

Y

F

A

A

R

Q

G

G

S

T

N

K

K

A

I

V

A

A

H

E

F

A

V

L

A

A

D

E

S

A

Y

K

A

D

T

T

-

Y

S

S

V

V

V

I

G

G

D

D

T

S

A

A

D

A

L

A

V

V

Q

E

R

K

V

F

G

G

L

L

D

A

E

D

E

K

I

M

T

S

F

H

I

I

S

S

T

T

G

G

A

A

S

S

L

L

E

E

L

F

L

M

E

E

G

G

K

K

I

E

L

L

P

P

G

G

V

V

K

A

P

A

L

L

S183 (≠ E187) modified: Phosphoserine
T299 (= T303) modified: Phosphothreonine

3pgkA The structure of yeast phosphoglycerate kinase at 0.25 nm resolution (see paper)
46% identity, 98% coverage: 4:391/396 of query aligns to 6:408/415 of 3pgkA

query
sites
3pgkA

L

L

G

S

I

V

K

Q

E

D

C

L

D

D

I

L

H

K

G

D

K

K

K

R

V

V

F

F

I

I

R

R

C

V

D

D

F

F

N

N

V

V

P

P

M

L

D

D

D

G

Y

K

G

-

N

K

I

I

T

T

D

S

D

N

R

Q

R
|
R

I

I

R

V

S

A

A

A

L

L

A

P

T

T

I

I

N

K

Y

Y

C

V

L

L

D

E

Q

H

K

H

C

P

A

R

-

Y

I

V

L

L

A

A

S

S

H

H

F

L

G

G

R

R

P

P

K

N

G

G

E

E

P

R

D

N

E

E

K

K

Y

Y

S

S

L

L

A

A

P

P

I

V

A

A

R

K

R

E

L

L

H

Q

Q

S

L

L

K

G

I

K

D

D

V

V

Q

T

V

F

A

L

D

N

D

D

V

C

V

V

G

G

E

P

S

E

A

V

L

E

K

A

L

A

A

V

H

K

E

A

L

S

K

A

N

P

G

G

E

S

V

V

L

I

L

L

E

E

N

N

L

L

R

R

F

Y

-

H

-

I

-

E

E

E

K

E

G

G

E

S

T

R

K

K

N

V

D

D

P

G

D

Q

L

K

S

V

A

K

A

A

-

S

-

K

-

E

-

D

-

V

-

Q

-

K

-

F

-

R

-

H

-

E

L

L

A

S

S

S

M

L

A

A

E

D

V

V

Y

Y

I

I

N

N

D

D

A

A

F

F

G

G

V

T

S

A

H

H

R

R

A

A

H

H

A

S

S

S

V

M

E

V

G

G

I

-

T

-

A

-

H

-

F

F

D

D

N

L

A

P

H

Q

K

R

A

A

G

G

F

F

L

L

Q

E

K

K

E

E

I

L

Q

K

F

Y

F

F

G

G

T

K

L

A

L

L

E

E

H

N

P

P

V

T

R

R

P

P

F

F

A

L

A

A

I

I

V

L

G

G

G
|
G

S
x
A

K
|
K

V

V

S

A

G

D

K

K

L

I

E

Q

A

L

L

I

I

D

N

N

L

L

P

D

K

K

V

V

D

D

K

S

M

I

L

I

I

I

G

G

M

M

A

A

F

F

T

T

F

F

L

K

K

K

A

V

L

L

G

E

H

N

-

T

D

E

V

I

G

G

N

D

S

S

L

I

V

F

E

D

D

K

D

A

L

V

I

G

P

P

E

E

A

I

L

A

K

K

I

L

M

M

E

E

E

K

A

A

K

K

R

A

L

K

G

G

V

V

K

E

F

V

Y

V

L

L

P

P

V

V

D

D

V

F

V

I

A

I

A

A

E

D

K

A

F
|
F

A

S

P

A

D

S

S

A

A

N

S

T

R

K

L

T

C

V

S

T

V

D

Q

K

E

E

-

G

I

I

P

P

S

A

G

G

W

W

M

Q

G

G

L
|
L

D

D

I

N

G

G

P

P

A

E

T

S

V

R

R

K

L

L

Y

F

R

A

Q

A

V

T

L

V

G

A

D

K

V

A

Q

T

T

V

I

I

L

L

W

W

N
|
N

G
|
G

P
|
P

M

P

G
|
G

V
|
V

Y

F

E

E

M

F

D

E

R

K

Y

F

A

A

R

A

G

G

S

T

N

K

K

A

I

L

A

L

H

D

F

E

V

V

A

V

D

K

S

S

Y

S

A

A

-

A

-

G

-

N

T

T

S

V

V

I

G

G

G
|
G

D
|
D

T

T

A

A

D

T

L

V

V

A

Q

K

R

K

V

Y

G

G

L

V

D

T

E

D

E

K

I

I

T

S

F

H

I

V

S

S

T

T

G

G

G
|
G

A

A

S

S

L

L

E

E

L

L

E

E

G

G

K

K

I

E

L

L

P

P

G

G

V

V

active site: R38 (= R37), K213 (= K201), G371 (= G354), G394 (= G377)
binding adenosine-5'-triphosphate: G211 (= G199), A212 (≠ S200), K213 (= K201), F289 (= F276), L311 (= L297), N334 (= N320), G335 (= G321), P336 (= P322), G338 (= G324), V339 (= V325), D372 (= D355)

P00560 Phosphoglycerate kinase; EC 2.7.2.3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
46% identity, 98% coverage: 4:391/396 of query aligns to 7:409/416 of P00560

query
sites
P00560

L

L

G

S

I

V

K

Q

E

D

C

L

D

D

I

L

H

K

G

D

K

K

K

R

V

V

F

F

I

I

R
|
R

C

V

D

D

F

F

N

N

V

V

P

P

M

L

D

D

D

G

Y

K

G

-

N

K

I

I

T

T

D

S

D

N

R

Q

R
|
R

I

I

R

V

S

A

A

A

L

L

A

P

T

T

I

I

N

K

Y

Y

C

V

L

L

D

E

Q

H

K

H

C

P

A

R

-

Y

I

V

L

L

A

A

S

S

H

H

F

L

G

G

R

R

P

P

K

N

G

G

E

E

P

R

D

N

E

E

K

K

Y

Y

S

S

L

L

A

A

P

P

I

V

A

A

R

K

R

E

L

L

H

Q

Q

S

L

L

K

G

I

K

D

D

V

V

Q

T

V

F

A

L

D

N

D

D

V

C

V

V

G

G

E

P

S

E

A

V

L

E

K

A

L

A

A

V

H

K

E

A

L

S

K

A

N

P

G

G

E

S

V

V

L

I

L

L

E

E

N

N

L

L

R
|
R

F

Y

-

H

-

I

-

E

E

E

K

E

G

G

E

S

T

R

K

K

N

V

D

D

P

G

D

Q

L

K

S

V

A

K

A

A

-

S

-

K

-

E

-

D

-

V

-

Q

-

K

-

F

-

R

-

H

-

E

L

L

A

S

S

S

M

L

A

A

E

D

V

V

Y

Y

I

I

N

N

D

D

A

A

F

F

G

G

V

T

S

A

H

H

R
|
R

A

A

H

H

A

S

S

S

V

M

E

V

G

G

I

-

T

-

A

-

H

-

F

F

D

D

N

L

A

P

H

Q

K

R

A

A

G

G

F

F

L

L

Q

E

K

K

E

E

I

L

Q

K

F

Y

F

F

G

G

T

K

L

A

L

L

E

E

H

N

P

P

V

T

R

R

P

P

F

F

A

L

A

A

I

I

V

L

G

G

S

A

K

K

V

V

S

A

G

D

K

K

L

I

E

Q

A

L

L

I

I

D

N

N

L

L

P

D

K

K

V

V

D

D

K

S

M

I

L

I

I

I

G

G

M

M

A

A

F

F

T

T

F

F

L

K

K

K

A

V

L

L

G

E

H

N

-

T

D

E

V

I

G

G

N

D

S

S

L

I

V

F

E

D

D

K

-

A

-

G

-

A

D

E

L

I

I

V

P

P

E

-

A

-

L

-

K

K

I

L

M

M

E

E

E

K

A

A

K

K

R

A

L

K

G

G

V

V

K

E

F

V

Y

V

L

L

P

P

V

V

D

D

V

F

V

I

A

I

A

A

E

D

K

A

F

F

A

S

P

A

D

D

S

A

A

N

S

T

R

K

L

T

C

V

S

T

V

D

Q

K

E

E

-

G

I

I

P

P

S

A

G

G

W

W

M

Q

G

G

L

L

D

D

I

N

G

G

P

P

A

E

T

S

V

R

R

K

L

L

Y

F

R

A

Q

A

V

T

L

V

G

A

D

K

V

A

Q

K

T

T

I

I

L

V

W

W

N

N

G

G

P

P

M

P

G

G

V

V

Y

F

E

E

M

F

D

E

R

K

Y

F

A

A

R

A

G

G

S

T

N

K

K

A

I

L

A

L

H

D

F

E

V

V

A

V

D

K

S

S

Y

S

A

A

-

A

-

G

-

N

T

T

S

V

V

I

G

G

D

D

T

T

A

A

D

T

L

V

V

A

Q

K

R

K

V

Y

G

G

L

V

D

T

E

D

E

K

I

I

T

S

F

H

I

V

S

S

T

T

G

G

A

A

S

S

L

L

E

E

L

L

E

E

G

G

K

K

I

E

L

L

P

P

G

G

V

V

R22 (= R19) mutation to K: 2-fold reduction of Vmax.; mutation to M: 7-fold reduction of Vmax.
R39 (= R37) binding substrate
R122 (= R119) binding substrate
R169 (= R152) binding substrate

1qpgA 3-phosphoglycerate kinase, mutation r65q (see paper)
46% identity, 98% coverage: 4:391/396 of query aligns to 6:408/415 of 1qpgA

query
sites
1qpgA

L

L

G

S

I

V

K

Q

E

D

C

L

D

D

I

L

H

K

G

D

K

K

K

R

V

V

F

F

I

I

R

R

C

V

D

D

F

F

N

N

V

V

P

P

M

L

D

D

D

G

Y

K

G

-

N

K

I

I

T

T

D

S

D

N

R

Q

R
|
R

I

I

R

V

S

A

A

A

L

L

A

P

T

T

I

I

N

K

Y

Y

C

V

L

L

D

E

Q

H

K

H

C

P

A

R

-

Y

I

V

L

L

A

A

S

S

H

H

F

L

G

G

R

Q

P

P

K

N

G

G

E

E

P

R

D

N

E

E

K

K

Y

Y

S

S

L

L

A

A

P

P

I

V

A

A

R

K

R

E

L

L

H

Q

Q

S

L

L

K

G

I

K

D

D

V

V

Q

T

V

F

A

L

D

N

D

D

V

C

V

V

G

G

E

P

S

E

A

V

L

E

K

A

L

A

A

V

H

K

E

A

L

S

K

A

N

P

G

G

E

S

V

V

L

I

L

L

E

E

N

N

L

L

R

R

F

Y

-

H

-

I

-

E

E

E

K

E

G

G

E

S

T

R

K

K

N

V

D

D

P

G

D

Q

L

K

S

V

A

K

A

A

-

S

-

K

-

E

-

D

-

V

-

Q

-

K

-

F

-

R

-

H

-

E

L

L

A

S

S

S

M

L

A

A

E

D

V

V

Y

Y

I

I

N

N

D

D

A

A

F

F

G

G

V

T

S

A

H

H

R

R

A

A

H

H

A

S

S

S

V

M

E

V

G

G

I

-

T

-

A

-

H

-

F

F

D

D

N

L

A

P

H

Q

K

R

A

A

G

G

F

F

L

L

Q

E

K

K

E

E

I

L

Q

K

F

Y

F

F

G

G

T

K

L

A

L

L

E

E

H

N

P

P

V

T

R

R

P

P

F

F

A

L

A

A

I

I

V

L

G

G

S

A

K
|
K

V

V

S

A

G

D

K

K

L

I

E

Q

A

L

L

I

I

D

N

N

L

L

P

D

K

K

V

V

D

D

K

S

M

I

L

I

I

I

G

G

G
|
G

M

M

A

A

F

F

T

T

F

F

L

K

K

K

A

V

L

L

G

E

H

N

-

T

D

E

V

I

G

G

N

D

S

S

L

I

V

F

E

D

D

K

-

A

-

G

-

A

D

E

L

I

I

V

P

P

E

-

A

-

L

-

K

K

I

L

M

M

E

E

E

K

A

A

K

K

R

A

L

K

G

G

V

V

K

E

F

V

Y

V

L

L

P

P

V

V

D

D

V

F

V

I

A

I

A

A

E

D

K

A

F

F

A

S

P

A

D

D

S

A

A

N

S

T

R

K

L

T

C

V

S

T

V

D

Q

K

E

E

-

G

I

I

P

P

S

A

G

G

W

W

M

Q

G

G

L

L

D

D

I

N

G

G

P

P

A

E

T

S

V

R

R

K

L

L

Y

F

R

A

Q

A

V

T

L

V

G

A

D

K

V

A

Q

K

T

T

I

I

L

V

W

W

N
|
N

G

G

P
|
P

M

P

G
|
G

V
|
V

Y
x
F

E
|
E

M

F

D

E

R

K

Y

F

A

A

R

A

G

G

S

T

N

K

K

A

I

L

A

L

H

D

F

E

V

V

A

V

D

K

S

S

Y

S

A

A

-

A

-

G

-

N

T

T

S

V

V

I

G

G

G
|
G

D
|
D

T
|
T

A

A

D

T

L

V

V

A

Q

K

R

K

V

Y

G

G

L

V

D

T

E

D

E

K

I

I

T

S

F

H

I

V

S

S

T

T

G

G

G
|
G

A

A

S

S

L

L

E

E

L

L

E

E

G

G

K

K

I

E

L

L

P

P

G

G

V

V

active site: R38 (= R37), K213 (= K201), G371 (= G354), G394 (= G377)
binding magnesium-5'-adenyly-imido-triphosphate: G235 (= G223), G236 (= G224), N334 (= N320), P336 (= P322), G338 (= G324), V339 (= V325), F340 (≠ Y326), E341 (= E327), G370 (= G353), G371 (= G354), D372 (= D355), T373 (= T356)

4feyA An x-ray structure of a putative phosphogylcerate kinase with bound adp from francisella tularensis subsp. Tularensis schu s4
45% identity, 99% coverage: 1:394/396 of query aligns to 1:386/392 of 4feyA

query
sites
4feyA

M

M

Q

S

L

F

L

L

G

T

I

L

K

K

E

D

C

V

D

D

I

L

H

K

G

D

K

K

V

V

F

L

I

V

R

R

C

V

D

D

F

F

N

N

V

V

P

P

M

V

D

K

D

D

Y

-

G

G

N

K

I

V

T

T

D

S

D

K

R

V

R
|
R

I

I

R

E

S

A

A

A

L

I

A

P

T

T

I

I

N

Q

Y

Y

C

I

L

L

D

D

Q

Q

K

G

C

G

A

A

I

V

I

I

L

L

A

M

S

S

H

H

F

L

G

G

R

R

P

P

-

T

K

E

G

G

E

E

P

Y

D

D

E

S

K

Q

Y

F

S

S

L

L

A

E

P

P

I

V

A

A

R

K

R

A

L

L

H

S

Q

E

L

I

K

N

I

K

D

P

V

V

Q

K

V

F

A

A

D

K

D

D

V

W

V

L

G

D

E

-

S

-

A

-

L

-

K

-

L

-

A

G

H

V

E

D

L

V

K

K

N

A

G

G

E

E

V

I

L

V

L

M

L

C

E

E

N

N

L

V

R

R

F

F

E

N

K

S

G

G

E

E

T

K

K

K

N

S

D

T

P

D

D

D

L

L

S

S

A

K

L

I

A

A

S

S

M

L

A

G

E

D

V

V

Y

F

I

V

N

M

D

D

A

A

F

F

G

A

V

T

S

A

H

H

R

R

A

A

H

Q

A

A

S

S

V

T

E

Y

G

G

I

V

T

A

A

K

H

Y

F

I

D

P

N

V

A

A

H

-

K

-

A

C

A

A

G

G

F

I

L

L

Q

T

K

N

E

E

I

I

Q

Q

F

A

F

L

G

E

T

K

L

A

L

L

E

K

H

S

P

P

V

K

R

K

P

P

F

M

A

A

I

I

V

V

G

G

G
|
G

S
|
S

K
|
K

V

V

S

S

G

T

K
|
K

L

L

E

S

A

V

L

L

I

N

N

N

L

L

P

D

K

K

V

V

D

E

K

I

M

L

L

I

I

V

G

G

G
|
G

G

G

M

I

A

A

F

N

T

T

F

F

L

I

K

K

A

A

L

E

G

G

H

F

D

D

V

V

G

G

N

N

S

S

L

L

V

Y

E

E

D

Q

D

D

L

L

I

V

P

A

E

E

A

A

L

T

K

E

I

I

M

L

E

A

E

K

A

A

K

K

R

A

L

L

G

G

V

V

K

N

F

I

Y

P

L

V

P

P

V

V

D

D

V

V

V

R

A

V

A

A

E

K

K

E

F

F

A

S

P

E

D

N

S

A

A

Q

S

A

R

I

L

I

C

K

S

K

V

V

Q

S

E

D

I

V

P

V

S

A

G

D

W

E

M

M

G

I

L

L

D

D

I

I

G

G

P

P

A

E

T

S

V

Q

R

K

L

I

Y

I

R

A

Q

E

V

L

L

L

G

K

D

S

V

A

Q

N

T

T

I

I

L

L

W

W

N

N

G

G

P

P

M

V

G
|
G

V
|
V

Y

F

E
|
E

M

F

D

D

R

N

Y

F

A

A

R

E

G

G

S

T

N

K

K

A

I

L

A

S

H

L

F

A

V

I

A

A

D

Q

S

S

Y

H

A

A

T

F

S

S

V

V

V

A

G

G

G
|
G

D
|
D

T

T

A

I

D

A

L

A

V

I

Q

E

R

K

V

F

G

G

L

I

D

K

E

D

E

Q

I

V

T

S

F

Y

I

I

S

S

T

T

G

A

G

G

G
|
G

A

A

S

F

L

L

E

E

L

F

L

L

E

E

G

G

K

K

I

K

L

L

P

P

G

A

V

I

K

E

P

I

L

L

active site: R36 (= R37), K193 (= K201), G346 (= G354), G369 (= G377)
binding adenosine-5'-diphosphate: G191 (= G199), S192 (= S200), K197 (= K205), G215 (= G223), G316 (= G324), V317 (= V325), E319 (= E327), D347 (= D355)

4axxA The catalytically active fully closed conformation of human phosphoglycerate kinase in complex with adp 3-phosphoglycerate and beryllium trifluoride
43% identity, 99% coverage: 4:394/396 of query aligns to 5:404/407 of 4axxA

query
sites
4axxA

L

L

G

T

I

L

K

D

E

K

C

L

D

D

I

V

H

K

G

G

K

K

K

R

V

V

F

V

I

M

R

R

C

V

D

D

F

F

N

N

V

V

P

P

M

M

D

K

D

N

Y

-

G

N

N

Q

I

I

T

T

D

N

R

Q

R
|
R

I

I

R

K

S

A

A

A

L

V

A

P

T

S

I

I

N

K

Y

F

C

C

L

L

D

D

Q

N

K

G

C

A

-

K

A

S

I

V

L

L

A

M

S

S

H

H

F

L

G

G

R

R

P

P

K

D

G

G

E

V

P

P

-

M

D

P

E

D

K

K

Y

Y

S

S

L

L

A

E

P

P

I

V

A

A

R

V

R

E

L

L

H

K

Q

S

L

L

K

G

I

K

D

D

V

V

Q

L

V

F

A

L

D

K

D

D

V

C

V

V

G

G

E

P

S

E

A

V

L

E

K

K

L

A

A

C

H

A

E

N

L

P

K

A

N

A

G

G

E

S

V

V

L

I

L

L

E

E

N

N

L

L

R

R

F

F

-

H

-

V

-

E

E

E

K

E

G

G

E

K

T

A

K

K

N

A

D

E

P

P

D

A

-

K

-

I

-

E

-

A

L

F

S

R

A

A

A

S

L

L

A

S

S

K

M

L

A

G

E

D

V

V

Y

Y

I

V

N

N

D

D

A

A

F

F

G

G

V

T

S

A

H

H

R

R

A

A

H

H

A

S

S

S

V

M

E

V

G

G

I

V

T

N

A

L

H

-

F

-

D

-

N

-

A

P

H

Q

K

K

A

A

A

G

G

G

F

F

L

L

L

M

Q

K

K

K

E

E

I

L

Q

N

F

Y

F

F

G

A

T

K

L

A

L

L

E

E

H

S

P

P

V

E

R

R

P

P

F

F

A

L

A

A

I

I

V

L

G

G

G
|
G

S
x
A

K
|
K

V

V

S

A

G

D

K
|
K

L

I

E

Q

A

L

L

I

I

N

N

N

L

M

L

L

P

D

K

K

V

V

D

N

K

E

M

M

L

I

I

I

G

G

G
|
G

M

M

A

A

F

F

T

T

F

F

L

L

K

K

A

V

L

L

G

N

H

N

-

M

D

E

V

I

G

G

N

T

S

S

L

L

V

F

E

D

D

E

L

G

I

A

P

K

E

I

A

V

L

K

K

D

I

L

M

M

E

S

E

K

A

A

K

E

R

K

L

N

G

G

V

V

K

K

F

I

Y

T

L

L

P

P

V

V

D

D

V

F

V

V

A

T

A

A

E

D

K

K

F

F

A

D

P

E

D

N

S

A

A

K

S

T

R

G

L

Q

C

A

S

T

V

V

Q

A

E

S

-

G

I

I

P

P

S

A

G

G

W

W

M

M

G

G

L

L

D

D

I

C

G

G

P

P

A

E

T

S

V

S

R

K

L

K

Y

Y

R

A

Q

E

V

A

L

V

G

T

D

R

V

A

Q

K

T

Q

I

I

L

V

W

W

N
|
N

G

G

P
|
P

M

V

G
|
G

V
|
V

Y

F

E
|
E

M

W

D

E

R

A

Y

F

A

A

R

R

G

G

S

T

N

K

K

A

I

L

A

M

H

D

F

E

V

V

A

V

D

K

S

A

Y

T

A

S

-

R

-

G

-

C

T

I

S

T

V

I

G

G

G
|
G

D
|
D

T
|
T

A

A

D

T

L

C

V

C

Q

A

R

K

V

W

G

N

L

T

D

E

E

D

E

K

I

V

T

S

F

H

I

V

S

S

T

T

G

G

G
|
G

A

A

S

S

L

L

E

E

L

L

E

E

G

G

K

K

I

V

L

L

P

P

G

G

V

V

K

D

P

A

L

L

active site: R37 (= R37), K206 (= K201), G364 (= G354), G387 (= G377)
binding adenosine-5'-diphosphate: G204 (= G199), A205 (≠ S200), K210 (= K205), G228 (= G223), G229 (= G224), N327 (= N320), P329 (= P322), G331 (= G324), V332 (= V325), E334 (= E327), G363 (= G353), G364 (= G354), D365 (= D355), T366 (= T356)
binding beryllium trifluoride ion: K206 (= K201), K210 (= K205), G363 (= G353)

P09041 Phosphoglycerate kinase 2; Phosphoglycerate kinase, testis specific; EC 2.7.2.3 from Mus musculus (Mouse) (see paper)
43% identity, 99% coverage: 4:394/396 of query aligns to 7:414/417 of P09041

query
sites
P09041

L

L

G

T

I

L

K

D

E

K

C

V

D

D

I

L

H

K

G

G

K

K

K

R

V

V

F

I

I

M

R

R

C

V

D

D

F

F

N

N

V

V

P

P

M

M

D

K

D

N

Y

-

G

N

N

Q

I

I

T

T

D

N

R

Q

R

R

I

I

R

K

S

A

A

A

L

I

A

P

T

S

I

I

N

K

Y

H

C

C

L

L

D

D

Q

N

K

G

C

A

-

K

A

S

I

V

L

L

A

M

S

S

H

H

F

L

G

G

R

R

P

P

K

D

G

G

E

I

P

P

-

M

D

P

E

D

K

K

Y

Y

S

S

L

L

A

E

P

P

I

V

A

A

R

D

R

E

L

L

H

K

Q

S

L

L

K

N

I

K

D

D

V

V

Q

I

V

F

A

L

D

K

D

D

V

C

V

V

G

G

E

P

S

E

A

V

L

E

K

Q

L

A

A

C

H

A

E

N

L

P

K

D

N

N

G

G

E

S

V

I

L

I

L

L

E

E

N

N

L

L

R

R

F

F

-

H

-

V

-

E

E

E

K

E

G

G

E

K

T

G

K

K

N

D

-

S

-

G

-

K

-

I

-

S

-

A

D

D

P

P

D

A

-

K

-

V

-

E

-

A

L

F

S

Q

A

A

A

S

L

L

A

S

S

K

M

L

A

G

E

D

V

V

Y

Y

I

V

N

N

D

D

A

A

F

F

G

G

V

T

S

A

H

H

R

R

A

A

H

H

A

S

S

S

V

T

E

V

G

G

I

V

T

N

A

L

H

-

F

-

D

-

N

-

A

P

H

Q

K

K

A

A

A

S

G

G

F

F

L

L

L

M

Q

K

K

K

E

E

I

L

Q

D

F

Y

F

F

G

S

T

K

L

A

L

L

E

E

H

K

P

P

V

E

R

R

P

P

F

F

A

L

A

A

I

I

V

L

G

G

S

A

K

K

V

V

S

K

G

D

K
|
K

L

I

E

Q

A

L

L

I

I

K

N

N

L

M

L

L

P

D

K

K

V

V

D

N

K

F

M

M

L

I

I

I

G

G

M

M

A

A

F

Y

T

T

F

F

L

L

K

K

A

E

L

L

G

K

H

N

-

M

D

Q

V

I

G

G

N

A

S

S

L

L

V

F

E

D

D

E

L

G

I

A

P

T

E

I

A

V

L

K

K

E

I

I

M

M

E

E

E

K

A

A

K

E

R

K

L

N

G

G

V

V

K

K

F

I

Y

V

L

F

P

P

V

V

D

D

V

F

V

V

A

T

A

G

E

D

K

K

F

F

A

D

P

E

D

N

S

A

A

K

S

V

R

G

L

Q

C

A

S

T

V

I

Q

E

E

S

-

G

I

I

P

P

S

S

G

G

W

W

M

M

G
|
G

L

L

D

D

I

C

G

G

P

P

A

E

T

S

V

I

R

K

L

I

Y

N

R

A

Q

Q

V

I

L

V

G

A

D

Q

V

A

Q

K

T

L

I

I

L

V

W

W

N

N

G

G

P

P

M

I

G

G

V

V

Y

F

E
|
E

M

W

D

D

R

A

Y

F

A

A

R

K

G

G

S

T

N

K

K

A

I

L

A

M

H

D

F

E

V

V

A

V

D

K

S

A

Y

T

A

S

T

N

-

G

-

C

-

V

S

T

V

I

G

G

G
|
G

D
|
D

T
|
T

A

A

D

T

L

C

V

C

Q

A

R

K

V

W

G

G

L

T

D

E

E

D

E

K

I

V

T

S

F

H

I

V

S

S

T

T

G

G

A

A

S

S

L

L

E

E

L

L

E

E

G

G

K

K

I

I

L

L

P

P

G

G

V

V

K

E

P

A

L

L

K220 (= K205) binding ATP
G313 (= G296) binding ATP
E344 (= E327) binding ATP
GGDT 373:376 (= GGDT 353:356) binding ATP

2paaA Crystal structure of phosphoglycerate kinase-2 bound to atp and 3pg (see paper)
43% identity, 99% coverage: 4:394/396 of query aligns to 3:410/413 of 2paaA

query
sites
2paaA

L

L

G

T

I

L

K

D

E

K

C

V

D

D

I

L

H

K

G

G

K

K

K

R

V

V

F

I

I

M

R

R

C

V

D

D

F

F

N

N

V

V

P

P

M

M

D

K

D

N

Y

-

G

N

N

Q

I

I

T

T

D

N

R

Q

R
|
R

I

I

R

K

S

A

A

A

L

I

A

P

T

S

I

I

N

K

Y

H

C

C

L

L

D

D

Q

N

K

G

C

A

-

K

A

S

I

V

L

L

A

M

S

S

H

H

F

L

G

G

R

R

P

P

K

D

G

G

E

I

P

P

-

M

D

P

E

D

K

K

Y

Y

S

S

L

L

A

E

P

P

I

V

A

A

R

D

R

E

L

L

H

K

Q

S

L

L

K

N

I

K

D

D

V

V

Q

I

V

F

A

L

D

K

D

D

V

C

V

V

G

G

E

P

S

E

A

V

L

E

K

Q

L

A

A

C

H

A

E

N

L

P

K

D

N

N

G

G

E

S

V

I

L

I

L

L

E

E

N

N

L

L

R

R

F

F

-

H

-

V

-

E

E

E

K

E

G

G

E

K

T

G

K

K

N

D

-

S

-

G

-

K

-

I

-

S

-

A

D

D

P

P

D

A

-

K

-

V

-

E

-

A

L

F

S

R

A

A

A

S

L

L

A

S

S

K

M

L

A

G

E

D

V

V

Y

Y

I

V

N

N

D

D

A

A

F

F

G

G

V

T

S

A

H

H

R

R

A

A

H

H

A

S

S

S

V

M

E

V

G

G

I

V

T

N

A

L

H

-

F

-

D

-

N

-

A

P

H

Q

K

K

A

A

A

S

G

G

F

F

L

L

L

M

Q

K

K

K

E

E

I

L

Q

D

F

Y

F

F

G

S

T

K

L

A

L

L

E

E

H

K

P

P

V

E

R

R

P

P

F

F

A

L

A

A

I

I

V

L

G

G

G
|
G

S
x
A

K
|
K

V

V

S

K

G

D

K
|
K

L

I

E

Q

A

L

L

I

I

K

N

N

L

M

L

L

P

D

K

K

V

V

D

N

K

F

M

M

L

I

I

I

G

G

G
|
G

M

M

A

A

F

Y

T

T

F

F

L

L

K

K

A

E

L

L

G

K

H

N

-

M

D

Q

V

I

G

G

N

A

S

S

L
|
L

V

F

E

D

D

E

L

G

I

A

P

T

E

I

A

V

L

K

K

E

I

I

M

M

E

E

E

K

A

A

K

E

R

K

L

N

G

G

V

V

K

K

F

I

Y

V

L

F

P

P

V

V

D

D

V

F

V

V

A

T

A

G

E

D

K

K

F

F

A

D

P

E

D

N

S

A

A

K

S

V

R

G

L

Q

C

A

S

T

V

I

Q

E

E

S

-

G

I

I

P

P

S

S

G

G

W

W

M

M

G
|
G

L
|
L

D

D

I

C

G

G

P

P

A

E

T

S

V

I

R

K

L

I

Y

N

R

A

Q

Q

V

I

L

V

G

A

D

Q

V

A

Q

K

T

L

I

I

L

V

W

W

N

N

G
|
G

P

P

M

I

G
|
G

V
|
V

Y

F

E
|
E

M

W

D

D

R

A

Y

F

A

A

R

K

G

G

S

T

N

K

K

A

I

L

A

M

H

D

F

E

V

V

A

V

D

K

S

A

Y

T

A

S

T

N

-

G

-

C

-

V

S

T

V

I

G

G

G
|
G

D
|
D

T

T

A

A

D

T

L

C

V

C

Q

A

R

K

V

W

G

G

L

T

D

E

E

D

E

K

I

V

T

S

F

H

I

V

S

S

T

T

G

G

G
|
G

A

A

S

S

L

L

E

E

L

L

E

E

G

G

K

K

I

I

L

L

P

P

G

G

V

V

K

E

P

A

L

L

active site: R35 (= R37), K212 (= K201), G370 (= G354), G393 (= G377)
binding adenosine-5'-triphosphate: G210 (= G199), A211 (≠ S200), K216 (= K205), G235 (= G224), L253 (= L241), G309 (= G296), L310 (= L297), G334 (= G321), G337 (= G324), V338 (= V325), E340 (= E327), D371 (= D355)

O60101 Phosphoglycerate kinase; EC 2.7.2.3 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
45% identity, 99% coverage: 4:394/396 of query aligns to 7:411/414 of O60101

query
sites
O60101

L

L

G

A

I

I

K

T

E

D

C

V

D

D

I

L

H

K

G

G

K

K

K

N

V

V

F

L

I

I

R

R

C

V

D

D

F

F

N

N

V

V

P

P

M

L

D

D

D

G

Y

-

G

D

N

R

I

I

T

T

D

N

R

A

R

R

I

I

R

V

S

G

A

A

L

L

A

P

T

T

I

I

N

K

Y

Y

C

A

L

L

D

E

Q

Q

K

Q

C

P

-

K

A

A

I

V

I

I

L

L

A

M

S

S

H

H

F

L

G

G

R

R

P

P

K

N

G

G

E

A

P

R

D

V

E

A

K

K

Y
|
Y

S
|
S

L

L

A

K

P

P

I

V

A

A

R

A

R

E

L

L

H

S

Q

K

L

L

K

G

I

K

D

P

V

V

Q

K

V

F

A

L

D

D

V

C

V

V

G

G

E

P

S

E

A

V

L

E

K

K

L

A

A

C

H

K

E

E

L

A

K

K

N

G

G

G

E

E

V

V

L

I

L

L

E

E

N

N

L

L

R

R

F

F

-

H

-

I

-

E

E

E

K

E

G

G

E

S

T

A

K

K

N

V

D

D

-

G

-

K

-

V

-

K

P

A

D

D

L

A

S
|
S

A

A

-

V

-

E

-

A

-

F

-

R

-

K

A

S

L

L

A

T

S

S

M

L

A

G

E

D

V

I

Y

F

I

V

N

N

D

D

A

A

F

F

G

G

V

T

S

A

H

H

R

R

A

A

H

H

A
x
S

S
|
S

V

M

E

V

G

G

I

V

T

-

A

-

H

-

F

-

D

-

N

D

A

L

H

P

K

R

A

V

A
x
S

G

G

F

F

L

L

L

M

Q

K

K

K

E

E

I

L

Q

D

F

Y

F

F

G

S

T

K

L

A

L

L

E

E

H

N

P

P

V

A

R

R

P

P

F

F

A

L

A

A

I

I

V

L

G

G

S

A

K

K

V

V

S

A

G

D

K

K

L

I

E

Q

A

L

L

I

I

D

N

N

L

L

P

D

K

K

V

V

D

N

K

R

M

L

L

I

I

I

G

C

G

G

M

M

A

A

F

F

T

T

F

F

L

L

K

K

A

V

L

L

-

N

G

G

H

M

D

K

V

I

G

G

N

D

S
|
S

L

L

V

F

E

D

D

E

D

A

L

G

I
x
S

P

K

E

N

A

V

L

E

K

S

I

M

M

M

E

A

E

K

A

A

K

K

R

K

L

N

G

N

V

V

K

E

F

V

Y

F

L

L

P

P

V

V

D

D

V

F

V

V

A

T

A

A

E

D

K

K

F

F

A

D

P

K

D

D

S

A

A

K

S

V

R

G

L

S

C

A

S
x
T

V

A

Q

E

E

E

-

G

I

I

P

P

S

D

G

G

W

W

M

M

G

G

L

L

D

D

I

C

G

G

P

P

A

K

T

S

V

S

R

A

L

K

Y

F

R

A

Q

E

V

V

L

I

G

T

D

T

V
x
S

Q

K

T

T

I

I

L

V

W

W

N

N

G

G

P

P

M

A

G

G

V

V

Y

F

E

E

M

F

D

D

R

N

Y

F

A

A

R

K

G

G

S

T

N

K

K
x
S

I

M

A

L

H

D

F

A

V

C

A

V

D

K

S

T

Y

C

A

E

T

A

S

G

-

N

-

V

V

I

V

V

G

G

D

D

T
|
T

A

A

D

T

L

V

V

A

Q

K

R

K

V

Y

G

G

L

K

D

E

E

D

E

A

I

L

T
x
S

F

H

I

V

S
|
S

T

T

G

G

A

A

S

S

L

L

E

E

L

L

E

E

G

G

K

K

I

A

L

L

P

P

G

G

V

V

K

V

P

A

L

L

Y75 (= Y72) modified: Phosphotyrosine
S76 (= S73) modified: Phosphoserine
S143 (= S132) modified: Phosphoserine
S172 (≠ A155) modified: Phosphoserine
S173 (= S156) modified: Phosphoserine
S183 (≠ A171) modified: Phosphoserine
S253 (= S240) modified: Phosphoserine
S260 (≠ I247) modified: Phosphoserine
T299 (≠ S286) modified: Phosphothreonine
S328 (≠ V314) modified: Phosphoserine
S351 (≠ K337) modified: Phosphoserine
T373 (= T356) modified: Phosphothreonine
S387 (≠ T370) modified: Phosphoserine
S390 (= S373) modified: Phosphoserine

Sites not aligning to the query:

412 modified: Phosphoserine
413 modified: Phosphoserine

2wzcA The catalytically active fully closed conformation of human phosphoglycerate kinase in complex with adp, 3pg and aluminium tetrafluoride (see paper)
43% identity, 99% coverage: 4:394/396 of query aligns to 5:402/405 of 2wzcA

query
sites
2wzcA

L

L

G

T

I

L

K

D

E

K

C

L

D

D

I

V

H

K

G

G

K

K

K

R

V

V

F

V

I

M

R

R

C

V

D

D

F

F

N

N

V

V

P

P

M

M

D

K

D

N

Y

-

G

N

N

Q

I

I

T

T

D

N

R

Q

R
|
R

I

I

R

K

S

A

A

A

L

V

A

P

T

S

I

I

N

K

Y

F

C

C

L

L

D

D

Q

N

K

G

C

A

-

K

A

S

I

V

L

L

A

M

S

S

H

H

F

L

G

G

R

R

P

P

K

D

G

G

E

V

P

P

-

M

D

P

E

D

K

K

Y

Y

S

S

L

L

A

E

P

P

I

V

A

A

R

V

R

E

L

L

H

K

Q

S

L

L

K

G

I

K

D

D

V

V

Q

L

V

F

A

L

D

K

D

D

V

C

V

V

G

G

E

P

S

E

A

V

L

E

K

K

L

A

A

C

H

A

E

N

L

P

K

A

N

A

G

G

E

S

V

V

L

I

L

L

E

E

N

N

L

L

R

R

F

F

E

H

-

V

-

E

-

G

K

K

G

G

E

E

T

P

K

A

N

K

D

I

P

E

D

A

L

F

S

R

A

A

A

S

L

L

A

S

S

K

M

L

A

G

E

D

V

V

Y

Y

I

V

N

N

D

D

A

A

F

F

G

G

V

T

S

A

H

H

R

R

A

A

H

H

A

S

S

S

V

M

E

V

G

G

I

V

T

N

A

L

H

-

F

-

D

-

N

-

A

P

H

Q

K

K

A

A

A

G

G

G

F

F

L

L

L

M

Q

K

K

K

E

E

I

L

Q

N

F

Y

F

F

G

A

T

K

L

A

L

L

E

E

H

S

P

P

V

E

R

R

P

P

F

F

A

L

A

A

I

I

V

L

G

G

G
|
G

S
x
A

K
|
K

V

V

S

A

G

D

K
|
K

L

I

E

Q

A

L

L

I

I

N

N

N

L

M

L

L

P

D

K

K

V

V

D

N

K

E

M

M

L

I

I

I

G

G

G
|
G

M

M

A

A

F

F

T

T

F

F

L

L

K

K

A

V

L

L

G

N

H

N

-

M

D

E

V

I

G

G

N

T

S

S

L

L

V

F

E

D

D

E

L

G

I

A

P

K

E

I

A

V

L

K

K

D

I

L

M

M

E

S

E

K

A

A

K

E

R

K

L

N

G

G

V

V

K

K

F

I

Y

T

L

L

P

P

V

V

D

D

V

F

V

V

A

T

A

A

E

D

K

K

F

F

A

D

P

E

D

N

S

A

A

K

S

T

R

G

L

Q

C

A

S

T

V

V

Q

A

E

S

-

G

I

I

P

P

S

A

G

G

W

W

M

M

G

G

L

L

D

D

I

C

G

G

P

P

A

E

T

S

V

S

R

K

L

K

Y

Y

R

A

Q

E

V

A

L

V

G

T

D

R

V

A

Q

K

T

Q

I

I

L

V

W

W

N
|
N

G

G

P
|
P

M

V

G
|
G

V
|
V

Y

F

E
|
E

M

W

D

E

R

A

Y

F

A

A

R

R

G

G

S

T

N

K

K

A

I

L

A

M

H

D

F

E

V

V

A

V

D

K

S

A

Y

T

A

S

-

R

-

G

-

C

T

I

S

T

V

I

G

G

G
|
G

D
|
D

T
|
T

A

A

D

T

L

C

V

C

Q

A

R

K

V

W

G

N

L

T

D

E

E

D

E

K

I

V

T

S

F

H

I

V

S

S

T

T

G

G

G
|
G

A

A

S

S

L

L

E

E

L

L

E

E

G

G

K

K

I

V

L

L

P

P

G

G

V

V

K

D

P

A

L

L

active site: R37 (= R37), K204 (= K201), G362 (= G354), G385 (= G377)
binding adenosine-5'-diphosphate: G202 (= G199), A203 (≠ S200), K204 (= K201), K208 (= K205), G226 (= G223), G227 (= G224), N325 (= N320), P327 (= P322), G329 (= G324), V330 (= V325), E332 (= E327), G361 (= G353), D363 (= D355), T364 (= T356)
binding tetrafluoroaluminate ion: R37 (= R37), K204 (= K201), K208 (= K205), G361 (= G353), G362 (= G354), G384 (= G376)

2wzbA The catalytically active fully closed conformation of human phosphoglycerate kinase in complex with adp, 3pg and magnesium trifluoride (see paper)
43% identity, 99% coverage: 4:394/396 of query aligns to 5:402/405 of 2wzbA

query
sites
2wzbA

L

L

G

T

I

L

K

D

E

K

C

L

D

D

I

V

H

K

G

G

K

K

K

R

V

V

F

V

I

M

R

R

C

V

D

D

F

F

N

N

V

V

P

P

M

M

D

K

D

N

Y

-

G

N

N

Q

I

I

T

T

D

N

R

Q

R
|
R

I

I

R

K

S

A

A

A

L

V

A

P

T

S

I

I

N

K

Y

F

C

C

L

L

D

D

Q

N

K

G

C

A

-

K

A

S

I

V

L

L

A

M

S

S

H

H

F

L

G

G

R

R

P

P

K

D

G

G

E

V

P

P

-

M

D

P

E

D

K

K

Y

Y

S

S

L

L

A

E

P

P

I

V

A

A

R

V

R

E

L

L

H

K

Q

S

L

L

K

G

I

K

D

D

V

V

Q

L

V

F

A

L

D

K

D

D

V

C

V

V

G

G

E

P

S

E

A

V

L

E

K

K

L

A

A

C

H

A

E

N

L

P

K

A

N

A

G

G

E

S

V

V

L

I

L

L

E

E

N

N

L

L

R

R

F

F

E

H

-

V

-

E

-

G

K

K

G

G

E

E

T

P

K

A

N

K

D

I

P

E

D

A

L

F

S

R

A

A

A

S

L

L

A

S

S

K

M

L

A

G

E

D

V

V

Y

Y

I

V

N

N

D

D

A

A

F

F

G

G

V

T

S

A

H

H

R

R

A

A

H

H

A

S

S

S

V

M

E

V

G

G

I

V

T

N

A

L

H

-

F

-

D

-

N

-

A

P

H

Q

K

K

A

A

A

G

G

G

F

F

L

L

L

M

Q

K

K

K

E

E

I

L

Q

N

F

Y

F

F

G

A

T

K

L

A

L

L

E

E

H

S

P

P

V

E

R

R

P

P

F

F

A

L

A

A

I

I

V

L

G

G

G
|
G

S
x
A

K
|
K

V

V

S

A

G

D

K
|
K

L

I

E

Q

A

L

L

I

I

N

N

N

L

M

L

L

P

D

K

K

V

V

D

N

K

E

M

M

L

I

I

I

G

G

G
|
G

M

M

A

A

F

F

T

T

F

F

L

L

K

K

A

V

L

L

G

N

H

N

-

M

D

E

V

I

G

G

N

T

S

S

L

L

V

F

E

D

D

E

L

G

I

A

P

K

E

I

A

V

L

K

K

D

I

L

M

M

E

S

E

K

A

A

K

E

R

K

L

N

G

G

V

V

K

K

F

I

Y

T

L

L

P

P

V

V

D

D

V

F

V

V

A

T

A

A

E

D

K

K

F

F

A

D

P

E

D

N

S

A

A

K

S

T

R

G

L

Q

C

A

S

T

V

V

Q

A

E

S

-

G

I

I

P

P

S

A

G

G

W

W

M

M

G

G

L

L

D

D

I

C

G

G

P

P

A

E

T

S

V

S

R

K

L

K

Y

Y

R

A

Q

E

V

A

L

V

G

T

D

R

V

A

Q

K

T

Q

I

I

L

V

W

W

N
|
N

G

G

P
|
P

M

V

G
|
G

V
|
V

Y

F

E
|
E

M

W

D

E

R

A

Y

F

A

A

R

R

G

G

S

T

N

K

K

A

I

L

A

M

H

D

F

E

V

V

A

V

D

K

S

A

Y

T

A

S

-

R

-

G

-

C

T

I

S

T

V

I

G

G

G
|
G

D
|
D

T
|
T

A

A

D

T

L

C

V

C

Q

A

R

K

V

W

G

N

L

T

D

E

E

D

E

K

I

V

T

S

F

H

I

V

S

S

T

T

G

G

G
|
G

A

A

S

S

L

L

E

E

L

L

E

E

G

G

K

K

I

V

L

L

P

P

G

G

V

V

K

D

P

A

L

L

active site: R37 (= R37), K204 (= K201), G362 (= G354), G385 (= G377)
binding adenosine-5'-diphosphate: G202 (= G199), A203 (≠ S200), K204 (= K201), K208 (= K205), G226 (= G223), G227 (= G224), N325 (= N320), P327 (= P322), G329 (= G324), V330 (= V325), E332 (= E327), G361 (= G353), D363 (= D355), T364 (= T356)
binding trifluoromagnesate: K204 (= K201), K208 (= K205), G361 (= G353), G384 (= G376), G385 (= G377)

2wzdA The catalytically active fully closed conformation of human phosphoglycerate kinase k219a mutant in complex with adp, 3pg and aluminium trifluoride (see paper)
43% identity, 99% coverage: 4:394/396 of query aligns to 5:402/405 of 2wzdA

query
sites
2wzdA

L

L

G

T

I

L

K

D

E

K

C

L

D

D

I

V

H

K

G

G

K

K

K

R

V

V

F

V

I

M

R

R

C

V

D

D

F

F

N

N

V

V

P

P

M

M

D

K

D

N

Y

-

G

N

N

Q

I

I

T

T

D

N

R

Q

R
|
R

I

I

R

K

S

A

A

A

L

V

A

P

T

S

I

I

N

K

Y

F

C

C

L

L

D

D

Q

N

K

G

C

A

-

K

A

S

I

V

L

L

A

M

S

S

H

H

F

L

G

G

R

R

P

P

K

D

G

G

E

V

P

P

-

M

D

P

E

D

K

K

Y

Y

S

S

L

L

A

E

P

P

I

V

A

A

R

V

R

E

L

L

H

K

Q

S

L

L

K

G

I

K

D

D

V

V

Q

L

V

F

A

L

D

K

D

D

V

C

V

V

G

G

E

P

S

E

A

V

L

E

K

K

L

A

A

C

H

A

E

N

L

P

K

A

N

A

G

G

E

S

V

V

L

I

L

L

E

E

N

N

L

L

R

R

F

F

E

H

-

V

-

E

-

G

K

K

G

G

E

E

T

P

K

A

N

K

D

I

P

E

D

A

L

F

S

R

A

A

A

S

L

L

A

S

S

K

M

L

A

G

E

D

V

V

Y

Y

I

V

N

N

D

D

A

A

F

F

G

G

V

T

S

A

H

H

R

R

A

A

H

H

A

S

S

S

V

M

E

V

G

G

I

V

T

N

A

L

H

-

F

-

D

-

N

-

A

P

H

Q

K

K

A

A

A

G

G

G

F

F

L

L

L

M

Q

K

K

K

E

E

I

L

Q

N

F

Y

F

F

G

A

T

K

L

A

L

L

E

E

H

S

P

P

V

E

R

R

P

P

F

F

A

L

A

A

I

I

V

L

G

G

G
|
G

S
x
A

K
|
K

V

V

S

A

G

D

K

A

L

I

E

Q

A

L

L

I

I

N

N

N

L

M

L

L

P

D

K

K

V

V

D

N

K

E

M

M

L

I

I

I

G

G

G
|
G

M

M

A

A

F

F

T

T

F

F

L

L

K

K

A

V

L

L

G

N

H

N

-

M

D

E

V

I

G

G

N

T

S

S

L

L

V

F

E

D

D

E

L

G

I

A

P

K

E

I

A

V

L

K

K

D

I

L

M

M

E

S

E

K

A

A

K

E

R

K

L

N

G

G

V

V

K

K

F

I

Y

T

L

L

P

P

V

V

D

D

V

F

V

V

A

T

A

A

E

D

K

K

F

F

A

D

P

E

D

N

S

A

A

K

S

T

R

G

L

Q

C

A

S

T

V

V

Q

A

E

S

-

G

I

I

P

P

S

A

G

G

W

W

M

M

G

G

L

L

D

D

I

C

G

G

P

P

A

E

T

S

V

S

R

K

L

K

Y

Y

R

A

Q

E

V

A

L

V

G

T

D

R

V

A

Q

K

T

Q

I

I

L

V

W

W

N
|
N

G

G

P
|
P

M

V

G
|
G

V
|
V

Y

F

E
|
E

M

W

D

E

R

A

Y

F

A

A

R

R

G

G

S

T

N

K

K

A

I

L

A

M

H

D

F

E

V

V

A

V

D

K

S

A

Y

T

A

S

-

R

-

G

-

C

T

I

S

T

V

I

G

G

G
|
G

D
|
D

T
|
T

A

A

D

T

L

C

V

C

Q

A

R

K

V

W

G

N

L

T

D

E

E

D

E

K

I

V

T

S

F

H

I

V

S

S

T

T

G

G

G
|
G

A

A

S

S

L

L

E

E

L

L

E

E

G

G

K

K

I

V

L

L

P

P

G

G

V

V

K

D

P

A

L

L

active site: R37 (= R37), K204 (= K201), G362 (= G354), G385 (= G377)
binding adenosine-5'-diphosphate: G202 (= G199), A203 (≠ S200), K204 (= K201), G226 (= G223), G227 (= G224), N325 (= N320), P327 (= P322), G329 (= G324), V330 (= V325), E332 (= E327), G361 (= G353), D363 (= D355), T364 (= T356)
binding aluminum fluoride: R37 (= R37), K204 (= K201), G361 (= G353), G362 (= G354), G384 (= G376)

2x15A The catalytically active fully closed conformation of human phosphoglycerate kinase in complex with adp and 1,3- bisphosphoglycerate
43% identity, 99% coverage: 4:394/396 of query aligns to 5:405/408 of 2x15A

query
sites
2x15A

L

L

G

T

I

L

K

D

E

K

C

L

D

D

I

V

H

K

G

G

K

K

K

R

V

V

F

V

I

M

R

R

C

V

D
|
D

F

F

N
|
N

V

V

P

P

M

M

D

K

D

N

Y

-

G

N

N

Q

I

I

T

T

D

N

R

Q

R
|
R

I

I

R

K

S

A

A

A

L

V

A

P

T

S

I

I

N

K

Y

F

C

C

L

L

D

D

Q

N

K

G

C

A

-

K

A

S

I

V

L

L

A

M

S

S

H
|
H

F

L

G

G

R
|
R

P

P

K

D

G

G

E

V

P

P

-

M

D

P

E

D

K

K

Y

Y

S

S

L

L

A

E

P

P

I

V

A

A

R

V

R

E

L

L

H

K

Q

S

L

L

K

G

I

K

D

D

V

V

Q

L

V

F

A

L

D

K

D

D

V

C

V

V

G

G

E

P

S

E

A

V

L

E

K

K

L

A

A

C

H

A

E

N

L

P

K

A

N

A

G

G

E

S

V

V

L

I

L

L

E

E

N

N

L

L

R
|
R

F

F

-

H

-

V

-

E

-

G

-

K

-

G

E

K

K

K

G

A

E

E

T

P

K

A

N

K

D

I

P

E

D

A

L

F

S

R

A

A

A

S

L

L

A

S

S

K

M

L

A

G

E

D

V

V

Y

Y

I

V

N

N

D

D

A

A

F

F

G

G

V

T

S

A

H

H

R
|
R

A

A

H

H

A

S

S

S

V

M

E

V

G

G

I

V

T

N

A

L

H

-

F

-

D

-

N

-

A

P

H

Q

K

K

A

A

A

G

G

G

F

F

L

L

L

M

Q

K

K

K

E

E

I

L

Q

N

F

Y

F

F

G

A

T

K

L

A

L

L

E

E

H

S

P

P

V

E

R

R

P

P

F

F

A

L

A

A

I

I

V

L

G

G

G
|
G

S
x
A

K
|
K

V

V

S

A

G

D

K
|
K

L

I

E

Q

A

L

L

I

I

N

N

N

L

M

L

L

P

D

K

K

V

V

D

N

K

E

M

M

L

I

I

I

G

G

G
|
G

M

M

A

A

F

F

T

T

F

F

L

L

K

K

A

V

L

L

G

N

H

N

-

M

D

E

V

I

G

G

N

T

S

S

L

L

V

F

E

D

D

E

L

G

I

A

P

K

E

I

A

V

L

K

K

D

I

L

M

M

E

S

E

K

A

A

K

E

R

K

L

N

G

G

V

V

K

K

F

I

Y

T

L

L

P

P

V

V

D

D

V

F

V

V

A

T

A

A

E

D

K

K

F

F

A

D

P

E

D

N

S

A

A

K

S

T

R

G

L

Q

C

A

S

T

V

V

Q

A

E

S

-

G

I

I

P

P

S

A

G

G

W

W

M

M

G

G

L

L

D

D

I

C

G

G

P

P

A

E

T

S

V

S

R

K

L

K

Y

Y

R

A

Q

E

V

A

L

V

G

T

D

R

V

A

Q

K

T

Q

I

I

L

V

W

W

N
|
N

G

G

P
|
P

M

V

G
|
G

V
|
V

Y

F

E
|
E

M

W

D

E

R

A

Y

F

A

A

R

R

G

G

S

T

N

K

K

A

I

L

A

M

H

D

F

E

V

V

A

V

D

K

S

A

Y

T

A

S

-

R

-

G

-

C

T

I

S

T

V

I

G

G

G
|
G

D
|
D

T
|
T

A

A

D

T

L

C

V

C

Q

A

R

K

V

W

G

N

L

T

D

E

E

D

E

K

I

V

T

S

F

H

I

V

S

S

T

T

G

G

G
|
G

A

A

S

S

L

L

E

E

L

L

E

E

G

G

K

K

I

V

L

L

P

P

G

G

V

V

K

D

P

A

L

L

active site: R37 (= R37), K207 (= K201), G365 (= G354), G388 (= G377)
binding adenosine-5'-diphosphate: G205 (= G199), A206 (≠ S200), K207 (= K201), K211 (= K205), G229 (= G223), G230 (= G224), N328 (= N320), P330 (= P322), G332 (= G324), V333 (= V325), E335 (= E327), G364 (= G353), G365 (= G354), D366 (= D355), T367 (= T356)
binding adenosine-5'-triphosphate: G205 (= G199), A206 (≠ S200), K207 (= K201), K211 (= K205), G229 (= G223), G230 (= G224), N328 (= N320), G332 (= G324), V333 (= V325), E335 (= E327), G364 (= G353), G365 (= G354), D366 (= D355), T367 (= T356), G387 (= G376), G388 (= G377)
binding 1,3-bisphosphoglyceric acid: D22 (= D21), N24 (= N23), R37 (= R37), H61 (= H60), R64 (= R63), R121 (= R119), R162 (= R152), K207 (= K201), K211 (= K205), G364 (= G353), G387 (= G376), G388 (= G377)

1vjcA Structure of pig muscle pgk complexed with mgatp (see paper)
42% identity, 99% coverage: 4:394/396 of query aligns to 6:413/416 of 1vjcA

query
sites
1vjcA

L

L

G

T

I

L

K

D

E

K

C

L

D

D

I

V

H

K

G

G

K

K

K

R

V

V

F

V

I

M

R

R

C

V

D

D

F

F

N

N

V

V

P

P

M

M

D

K

D

N

Y

-

G

N

N

Q

I

I

T

T

D

N

R

Q

R
|
R

I

I

R

K

S

A

A

A

L

I

A

P

T

S

I

I

N

K

Y

F

C

C

L

L

D

D

Q

N

K

G

C

A

-

K

A

S

I

V

L

L

A

M

S

S

H

H

F

L

G

G

R

R

P

P

K

D

G

G

E

I

P

P

-

M

D

P

E

D

K

K

Y

Y

S

S

L

L

A

E

P

P

I

V

A

A

R

V

R

E

L

L

H

K

Q

S

L

L

K

G

I

K

D

D

V

V

Q

L

V

F

A

L

D

K

D

D

V

C

V

V

G

G

E

P

S

E

A

V

L

E

K

K

L

A

A

C

H

A

E

D

L

P

K

A

N

A

G

G

E

S

V

V

L

I

L

L

E

E

N

N

L

L

R

R

F

F

-

H

-

V

-

E

E

E

K

E

G

G

E

K

T

G

K

K

N

D

-

A

-

S

-

G

-

S

-

K

-

V

-

K

-

A

D

D

P

P

D

A

-

K

-

I

-

E

-

A

L

F

S

R

A

A

A

S

L

L

A

S

S

K

M

L

A

G

E

D

V

V

Y

Y

I

V

N

N

D

D

A

A

F

F

G

G

V

T

S

A

H

H

R

R

A

A

H

H

A

S

S

S

V

M

E

V

G

G

I

V

T

N

A

L

H

-

F

-

D

-

N

-

A

P

H

K

K

K

A

A

A

G

G

G

F

F

L

L

L

M

Q

K

K

K

E

E

I

L

Q

N

F

Y

F

F

G

A

T

K

L

A

L

L

E

E

H

S

P

P

V

E

R

R

P

P

F

F

A

L

A

A

I

I

V

L

G

G

G
|
G

S

A

K
|
K

V

V

S

A

G

D

K
|
K

L

I

E

Q

A

L

L

I

I

N

N

N

L

M

L

L

P

D

K

K

V

V

D

N

K

E

M

M

L

I

I

I

G

G

G
|
G

M

M

A

A

F

F

T

T

F

F

L

L

K

K

A

V

L

L

G

N

H

N

-

M

D

E

V

I

G

G

N

T

S

S

L

L

V

F

E

D

D

E

L

G

I

S

P

K

E

I

A

V

L

K

K

D

I

L

M

M

E

S

E

K

A

A

K

E

R

K

L

N

G

G

V

V

K

K

F

I

Y

T

L

L

P

P

V

V

D

D

V

F

V

V

A

T

A

A

E

D

K

K

F

F

A

D

P

E

D

N

S

A

A

K

S

T

R

G

L

Q

C

A

S

T

V

V

Q

A

E

S

-

G

I

I

P

P

S

A

G

G

W

W

M

M

G

G

L

L

D

D

I

C

G

G

P

P

A

E

T

S

V

S

R

K

L

K

Y

Y

R

S

Q

E

V

A

L

V

G

A

D

R

V

A

Q

K

T

Q

I

I

L

V

W

W

N

N

G

G

P

P

M

V

G
|
G

V
|
V

Y

F

E
|
E

M

W

D

E

R

A

Y

F

A

A

R

Q

G

G

S

T

N

K

K

A

I

L

A

M

H

D

F

E

V

V

A

V

D

K

S

A

Y

T

A

S

-

R

-

G

-

C

T

I

S

T

V

I

G

G

G
|
G

D
|
D

T

T

A

A

D

T

L

C

V

C

Q

A

R

K

V

W

G

N

L

T

D

E

E

D

E

K

I

V

T

S

F

H

I

V

S

S

T

T

G

G

G
|
G

A

A

S

S

L

L

E

E

L

L

E

E

G

G

K

K

I

V

L

L

P

P

G

G

V

V

K

D

P

A

L

L

active site: R38 (= R37), K215 (= K201), G373 (= G354), G396 (= G377)
binding adenosine-5'-triphosphate: G213 (= G199), K219 (= K205), G238 (= G224), G340 (= G324), V341 (= V325), E343 (= E327), D374 (= D355)

2y3iA The structure of the fully closed conformation of human pgk in complex with l-adp, 3pg and the tsa aluminium tetrafluoride (see paper)
42% identity, 99% coverage: 4:394/396 of query aligns to 5:412/414 of 2y3iA

query
sites
2y3iA

L

L

G

T

I

L

K

D

E

K

C

L

D

D

I

V

H

K

G

G

K

K

K

R

V

V

F

V

I

M

R

R

C

V

D

D

F

F

N

N

V

V

P

P

M

M

D

K

D

N

Y

-

G

N

N

Q

I

I

T

T

D

N

R

Q

R
|
R

I

I

R

K

S

A

A

A

L

V

A

P

T

S

I

I

N

K

Y

F

C

C

L

L

D

D

Q

N

K

G

C

A

-

K

A

S

I

V

L

L

A

M

S

S

H

H

F

L

G

G

R

R

P

P

K

D

G

G

E

V

P

P

-

M

D

P

E

D

K

K

Y

Y

S

S

L

L

A

E

P

P

I

V

A

A

R

V

R

E

L

L

H

K

Q

S

L

L

K

G

I

K

D

D

V

V

Q

L

V

F

A

L

D

K

D

D

V

C

V

V

G

G

E

P

S

E

A

V

L

E

K

K

L

A

A

C

H

A

E

N

L

P

K

A

N

A

G

G

E

S

V

V

L

I

L

L

E

E

N

N

L

L

R

R

F

F

E

H

K

V

G

E

E

E

T

E

K

G

N

K

D

G

P

K

D

D

L

A

S

S

-

G

-

N

-

K

-

V

-

K

-

A

-

E

-

P

-

A

-

K

-

I

-

E

-

A

-

F

-

R

A

A

A

S

L

L

A

S

S

K

M

L

A

G

E

D

V

V

Y

Y

I

V

N

N

D

D

A

A

F

F

G

G

V

T

S

A

H

H

R

R

A

A

H

H

A

S

S

S

V

M

E

V

G

G

I

V

T

N

A

L

H

-

F

-

D

-

N

-

A

P

H

Q

K

K

A

A

A

G

G

G

F

F

L

L

L

M

Q

K

K

K

E

E

I

L

Q

N

F

Y

F

F

G

A

T

K

L

A

L

L

E

E

H

S

P

P

V

E

R

R

P

P

F

F

A

L

A

A

I

I

V

L

G

G

G
|
G

S
x
A

K
|
K

V

V

S

A

G

D

K

K

L

I

E

Q

A

L

L

I

I

N

N

N

L

M

L

L

P

D

K

K

V

V

D

N

K

E

M

M

L

I

I

I

G

G

M

M

A

A

F

F

T

T

F

F

L

L

K

K

A

V

L

L

G

N

H

N

-

M

D

E

V

I

G

G

N

T

S

S

L

L

V

F

E

D

D

E

L

G

I

A

P

K

E

I

A

V

L

K

K

D

I

L

M

M

E

S

E

K

A

A

K

E

R

K

L

N

G

G

V

V

K

K

F

I

Y

T

L

L

P

P

V

V

D

D

V

F

V

V

A

T

A

A

E

D

K

K

F
|
F

A

D

P

E

D

N

S

A

A

K

S

T

R

G

L

Q

C

A

S

T

V

V

Q

A

E

S

-

G

I

I

P

P

S

A

G

G

W

W

M

M

G

G

L

L

D

D

I

C

G

G

P

P

A

E

T

S

V

S

R

K

L

K

Y

Y

R

A

Q

E

V

A

L

V

G

T

D

R

V

A

Q

K

T

Q

I

I

L

V

W

W

N
|
N

G

G

P

P

M

V

G
|
G

V
|
V

Y

F

E
|
E

M

W

D

E

R

A

Y

F

A

A

R

R

G

G

S

T

N

K

K

A

I

L

A

M

H

D

F

E

V

V

A

V

D

K

S

A

Y

T

A

S

-

R

-

G

-

C

T

I

S

T

V

I

G

G

G
|
G

D
|
D

T
|
T

A

A

D

T

L

C

V

C

Q

A

R

K

V

W

G

N

L

T

D

E

E

D

E

K

I

V

T

S

F

H

I

V

S

S

T

T

G

G

G
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G

A

A

S

S

L

L

E

E

L

L

E

E

G

G

K

K

I

V

L

L

P

P

G

G

V

V

K

D

P

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L

L

active site: R37 (= R37), K214 (= K201), G372 (= G354), G395 (= G377)
binding tetrafluoroaluminate ion: K214 (= K201), G371 (= G353), G372 (= G354), G394 (= G376)
binding l-adenosine-5'-diphosphate: G212 (= G199), A213 (≠ S200), F290 (= F276), N335 (= N320), G339 (= G324), V340 (= V325), E342 (= E327), G371 (= G353), G372 (= G354), D373 (= D355), T374 (= T356)

P00558 Phosphoglycerate kinase 1; Cell migration-inducing gene 10 protein; Primer recognition protein 2; PRP 2; EC 2.7.2.3 from Homo sapiens (Human) (see 16 papers)
42% identity, 99% coverage: 4:394/396 of query aligns to 7:414/417 of P00558

query
sites
P00558

L

L

G

T

I

L

K

D

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K

C

L

D

D

I

V

H

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G

G

K

K

K

R

V

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R

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C

V

D
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D

F
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F

N
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N

V

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P

M

M

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K

D

N

Y

-

G

N

N

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R
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R

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R

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S

A

A

A

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A

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I

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C

C

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L

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D

Q

N

K

G

C

A

-

K

A

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S

H
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H

F
x
L

G
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G

R
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R

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G

G

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P

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M

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D

K

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Y

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A

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L

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x
K

D

D

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C

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V

G

G

E

P

S

E

A

V

L

E

K

K

L

A

A

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H

A

E

N

L

P

K

A

N

A

G

G

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V

L

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L

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E

N

N

L

L

R
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R

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G

E

E

E

T

E

K

G

N
x
K

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K

D

D

L

A

S

S

-

G

-

N

-

K

-

V

-

K

-

A

-

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-

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-

A

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-

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-

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-

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L

A

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A
x
G

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Y

Y

I

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N

N

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D

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A

F

F

G

G

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H

H

R
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R

A

A

H

H

A

S

S

S

V

M

E

V

G

G

I

V

T

N

A

L

H

-

F

-

D

-

N

-

A

P

H

Q

K

K

A

A

A

G

G

G

F

F

L

L

L

M

Q
x
K

K

K

E

E

I

L

Q

N

F

Y

F

F

G

A

T

K

L

A

L

L

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E

H

S

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R
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R

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P

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F

A

L

A

A

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I

V

L

G

G

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A

K
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K

V

V

S

A

G

D

K
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K

L

I

E

Q

A

L

L

I

I

N

N

N

L

M

L

L

P

D

K

K

V

V

D

N

K

E

M

M

L

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I

I

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G

M

M

A

A

F

F

T

T

F

F

L

L

K

K

A

V

L

L

G

N

H

N

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M

D
x
E

V

I

G

G

N

T

S

S

L

L

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A

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A
x
V

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x
D

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E

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A

A

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K

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N

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L

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D
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D

V

F

V

V

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T

A

A

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D

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K

F

F

A

D

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D

N

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A

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V

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W

W

M

M

G
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G

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D

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C

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A

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Y

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N

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E

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E

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A

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F

A

A

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R

G

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x
T

N

K

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A

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A

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V

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K

S

A

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R

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G

-

C

T

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G

G

G
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G

D
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D

T
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T

A

A

D

T

L

C

V

C

Q

A

R

K

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W

G

N

L

T

D

E

E

D

E

K

I

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T

S

F

H

I

V

S

S

T

T

G

G

A

A

S

S

L

L

E

E

L

L

E

E

G

G

K

K

I

V

L

L

P

P

G

G

V

V

K

D

P

A

L

L

DFN 24:26 (= DFN 21:23) binding substrate
R39 (= R37) binding substrate
HLGR 63:66 (≠ HFGR 60:63) binding substrate
L88 (= L84) to P: in PGK1D; with congenital non-spherocytic anemia; variant Matsue; dbSNP:rs137852531
K97 (≠ D93) modified: N6-(2-hydroxyisobutyryl)lysine; alternate
R123 (= R119) binding substrate
K131 (≠ N127) modified: N6-malonyllysine; alternate
G158 (≠ A139) to V: in PGK1D; with chronic hemolytic anemia; variant Shizuoka; dbSNP:rs137852532
D164 (= D145) to V: in PGK1D; with chronic hemolytic anemia and intellectual disability; variant Amiens; dbSNP:rs137852538
R171 (= R152) binding substrate
K191 (≠ Q176) natural variant: Missing (in PGK1D; with chronic hemolytic anemia; variant Alabama)
R206 (= R191) to P: in PGK1D; with chronic hemolytic anemia; variant Uppsala; dbSNP:rs137852529
K216 (= K201) modified: N6-(2-hydroxyisobutyryl)lysine
K220 (= K205) binding ATP; modified: N6-(2-hydroxyisobutyryl)lysine
E252 (≠ D236) to A: in PGK1D; with chronic hemolytic anemia; variant Antwerp
V266 (≠ A250) to M: in PGK1D; with chronic non-spherocytic hemolytic anemia; variant Tokyo; dbSNP:rs431905501
D268 (≠ K252) to N: in Munchen; 21% of activity; dbSNP:rs137852528
D285 (= D269) to V: in PGK1D; with chronic hemolytic anemia; variant Herlev; 50% of activity; dbSNP:rs137852535
G313 (= G296) binding ATP
D315 (= D298) to N: in PGK1D; with rhabdomyolysis; variant Creteil; dbSNP:rs2149136994
C316 (≠ I299) to R: in PGK1D; with chronic hemolytic anemia; variant Michigan; dbSNP:rs137852533
K323 (≠ L306) modified: N6-(2-hydroxyisobutyryl)lysine
E344 (= E327) binding ATP
T352 (≠ S335) to N: in dbSNP:rs137852530
GGDT 373:376 (= GGDT 353:356) binding ATP

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

Query Sequence

>WP_013459152.1 NCBI__GCF_000183725.1:WP_013459152.1
MQLLGIKECDIHGKKVFIRCDFNVPMDDYGNITDDRRIRSALATINYCLDQKCAIILASH
FGRPKGEPDEKYSLAPIARRLHQLLKIDVQVADDVVGESALKLAHELKNGEVLLLENLRF
EKGETKNDPDLSAALASMAEVYINDAFGVSHRAHASVEGITAHFDNAHKAAGFLLQKEIQ
FFGTLLEHPVRPFAAIVGGSKVSGKLEALINLLPKVDKMLIGGGMAFTFLKALGHDVGNS
LVEDDLIPEALKIMEEAKRLGVKFYLPVDVVAAEKFAPDSASRLCSVQEIPSGWMGLDIG
PATVRLYRQVLGDVQTILWNGPMGVYEMDRYARGSNKIAHFVADSYATSVVGGGDTADLV
QRVGLDEEITFISTGGGASLELLEGKILPGVKPLLK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory