SitesBLAST
Comparing WP_013459726.1 NCBI__GCF_000183725.1:WP_013459726.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3du4A Crystal structure of 7-keto-8-aminopelargonic acid bound 7,8- diaminopelargonic acid synthase in bacillus subtilis (see paper)
39% identity, 97% coverage: 14:431/431 of query aligns to 13:443/448 of 3du4A
- active site: F17 (≠ C18), Y146 (= Y147), E217 (= E212), D251 (= D246), A254 (≠ L249), K280 (= K275), A417 (≠ Y405)
- binding 7-keto-8-aminopelargonic acid: L82 (≠ A84), Y146 (= Y147), G315 (≠ S310), S317 (= S312), R410 (= R398)
- binding pyridoxal-5'-phosphate: S112 (≠ N114), G113 (= G115), A114 (≠ S116), Y146 (= Y147), H147 (= H148), E217 (= E212), D251 (= D246), V253 (≠ I248), A254 (≠ L249), K280 (= K275), H316 (= H311), S317 (= S312)
P53555 L-Lysine--8-amino-7-oxononanoate transaminase; 7,8-diamino-pelargonic acid aminotransferase; DAPA AT; DAPA aminotransferase; 7,8-diaminononanoate synthase; DANS; Diaminopelargonic acid synthase; L-Lysine--8-amino-7-oxononanoate aminotransferase; EC 2.6.1.105 from Bacillus subtilis (strain 168) (see paper)
39% identity, 97% coverage: 14:431/431 of query aligns to 13:443/448 of P53555
- GA 113:114 (≠ GS 115:116) binding
- Y146 (= Y147) binding
- K280 (= K275) modified: N6-(pyridoxal phosphate)lysine
- G315 (≠ S310) binding
- HS 316:317 (= HS 311:312) binding
- R410 (= R398) binding
6zhkA Crystal structure of adenosylmethionine-8-amino-7-oxononanoate aminotransferase from methanocaldococcus jannaschii dsm 2661
37% identity, 97% coverage: 10:428/431 of query aligns to 9:433/438 of 6zhkA
6wnnA Bacillus subtilis bioa in complex with amino donor l-lys
38% identity, 97% coverage: 14:431/431 of query aligns to 10:415/420 of 6wnnA
- active site: F14 (≠ C18), Y143 (= Y147), D223 (= D246), K252 (= K275)
- binding (2S)-2-amino-6-[[3-hydroxy-2-methyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]hexanoic acid: F14 (≠ C18), W51 (= W56), S109 (≠ N114), G110 (= G115), A111 (≠ S116), Y143 (= Y147), H144 (= H148), D223 (= D246), V225 (≠ I248), K252 (= K275), R382 (= R398)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: H288 (= H311), S289 (= S312)
3dodA Crystal structure of plp bound 7,8-diaminopelargonic acid synthase in bacillus subtilis (see paper)
38% identity, 97% coverage: 14:431/431 of query aligns to 11:412/417 of 3dodA
- active site: F15 (≠ C18), E186 (= E212), D220 (= D246), A223 (≠ L249), K249 (= K275), A386 (≠ Y405)
- binding pyridoxal-5'-phosphate: W52 (= W56), S110 (≠ N114), G111 (= G115), A112 (≠ S116), D220 (= D246), V222 (≠ I248), K249 (= K275), H285 (= H311), S286 (= S312)
6ed7A Crystal structure of 7,8-diaminopelargonic acid synthase bound to inhibitor mac13772
38% identity, 98% coverage: 8:430/431 of query aligns to 7:423/429 of 6ed7A
- active site: Y17 (≠ C18), Y144 (= Y147), D245 (= D246), K274 (= K275)
- binding 2-[(2-nitrophenyl)sulfanyl]acetohydrazide: Y17 (≠ C18), W52 (= W55), W52 (= W55), Y144 (= Y147), D147 (≠ E150), A217 (= A218), K274 (= K275), R391 (= R398), F393 (≠ L400), F393 (≠ L400)
- binding pyridoxal-5'-phosphate: G112 (= G115), S113 (= S116), Y144 (= Y147), H145 (= H148), D245 (= D246), I247 (= I248), K274 (= K275)
P12995 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase; 7,8-diamino-pelargonic acid aminotransferase; DAPA AT; DAPA aminotransferase; 7,8-diaminononanoate synthase; DANS; Diaminopelargonic acid synthase; EC 2.6.1.62 from Escherichia coli (strain K12) (see 4 papers)
38% identity, 98% coverage: 8:430/431 of query aligns to 7:423/429 of P12995
- Y17 (≠ C18) mutation to F: Severely reduces the aminotransferase activity.
- W52 (= W55) binding
- GS 112:113 (= GS 115:116) binding
- Y144 (= Y147) mutation to F: Severely reduces the aminotransferase activity.
- D147 (≠ E150) mutation to N: Loss of aminotransferase activity.
- D245 (= D246) binding
- R253 (= R254) mutation to A: Has only a small effect on the rate of reaction with DAPA.; mutation to K: Increases aminotransferase activity toward SAM.; mutation to M: Loss of aminotransferase activity.; mutation to Q: Increases aminotransferase activity toward SAM.
- K274 (= K275) binding ; modified: N6-(pyridoxal phosphate)lysine
- G307 (≠ S310) binding
- PT 308:309 (≠ HS 311:312) binding
- R391 (= R398) binding ; mutation to A: Reduces aminotransferase activity.
1mlzA Crystal structure of 7,8-diaminopelargonic acid synthase in complex with the trans-isomer of amiclenomycin. (see paper)
38% identity, 98% coverage: 8:430/431 of query aligns to 7:422/427 of 1mlzA
- active site: Y17 (≠ C18), Y144 (= Y147), E210 (= E212), D244 (= D246), A247 (≠ L249), K273 (= K275), Y397 (= Y405)
- binding pyridoxal-5'-phosphate: G112 (= G115), S113 (= S116), Y144 (= Y147), H145 (= H148), D244 (= D246), I246 (= I248), K273 (= K275), P307 (≠ H311), T308 (≠ S312)
- binding trans-amiclenomycin: W52 (= W55), W53 (= W56), Y144 (= Y147), K273 (= K275), R390 (= R398), F392 (≠ L400)
1mlyA Crystal structure of 7,8-diaminopelargonic acid synthase in complex with the cis isomer of amiclenomycin (see paper)
38% identity, 98% coverage: 8:430/431 of query aligns to 7:422/427 of 1mlyA
- active site: Y17 (≠ C18), Y144 (= Y147), E210 (= E212), D244 (= D246), A247 (≠ L249), K273 (= K275), Y397 (= Y405)
- binding cis-amiclenomycin: W52 (= W55), W53 (= W56), K273 (= K275), R390 (= R398), F392 (≠ L400)
- binding pyridoxal-5'-phosphate: G112 (= G115), S113 (= S116), Y144 (= Y147), H145 (= H148), D244 (= D246), I246 (= I248), K273 (= K275), P307 (≠ H311), T308 (≠ S312)
6erkA Crystal structure of diaminopelargonic acid aminotransferase from psychrobacter cryohalolentis (see paper)
36% identity, 98% coverage: 1:424/431 of query aligns to 1:409/420 of 6erkA
1qj3A Crystal structure of 7,8-diaminopelargonic acid synthase in complex with 7-keto-8-aminopelargonic acid (see paper)
38% identity, 98% coverage: 8:430/431 of query aligns to 7:411/416 of 1qj3A
- active site: Y17 (≠ C18), Y144 (= Y147), E201 (= E212), D235 (= D246), A238 (≠ L249), K264 (= K275), Y386 (= Y405)
- binding 7-keto-8-aminopelargonic acid: Y17 (≠ C18), W52 (= W55), Y144 (= Y147), K264 (= K275), R379 (= R398), F381 (≠ L400)
- binding pyridoxal-5'-phosphate: G112 (= G115), S113 (= S116), Y144 (= Y147), H145 (= H148), G146 (= G149), D235 (= D246), I237 (= I248), A238 (≠ L249), K264 (= K275)
1dtyA Crystal structure of adenosylmethionine-8-amino-7-oxonanoate aminotransferase with pyridoxal phosphate cofactor.
38% identity, 98% coverage: 8:430/431 of query aligns to 7:423/429 of 1dtyA
- active site: Y17 (≠ C18), Y144 (= Y147), E211 (= E212), D245 (= D246), A248 (≠ L249), K274 (= K275), Y398 (= Y405)
- binding pyridoxal-5'-phosphate: G112 (= G115), S113 (= S116), Y144 (= Y147), H145 (= H148), D245 (= D246), I247 (= I248), K274 (= K275)
4cxqA Mycobaterium tuberculosis transaminase bioa complexed with substrate kapa (see paper)
33% identity, 97% coverage: 5:424/431 of query aligns to 5:418/427 of 4cxqA
- active site: Y18 (≠ C18), Y149 (= Y147), E212 (= E212), D246 (= D246), A249 (≠ L249), K275 (= K275), Y399 (= Y405)
- binding 7-keto-8-aminopelargonic acid: W56 (= W55), Y149 (= Y147), G308 (≠ S310), T310 (≠ S312), R392 (= R398)
- binding pyridoxal-5'-phosphate: G116 (= G115), S117 (= S116), Y149 (= Y147), H150 (= H148), G151 (= G149), E212 (= E212), D246 (= D246), I248 (= I248), K275 (= K275), P309 (≠ H311), T310 (≠ S312)
3tfuA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, post-reaction complex with a 3,6- dihydropyrid-2-one heterocycle inhibitor (see paper)
33% identity, 97% coverage: 5:424/431 of query aligns to 6:410/417 of 3tfuA
- active site: Y19 (≠ C18), Y151 (= Y147), E204 (= E212), D238 (= D246), A241 (≠ L249), K267 (= K275), Y391 (= Y405)
- binding [5-hydroxy-4-({[6-(3-hydroxypropyl)-2-oxo-1,2-dihydropyridin-3-yl]amino}methyl)-6-methylpyridin-3-yl]methyl dihydrogen phosphate: W58 (= W55), G118 (= G115), S119 (= S116), Y151 (= Y147), H152 (= H148), D238 (= D246), I240 (= I248), K267 (= K275), P301 (≠ H311), T302 (≠ S312)
4w1vA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with a thiazole inhibitor (see paper)
33% identity, 97% coverage: 5:424/431 of query aligns to 5:416/425 of 4w1vA
- active site: Y18 (≠ C18), Y147 (= Y147), E210 (= E212), D244 (= D246), A247 (≠ L249), K273 (= K275), Y397 (= Y405)
- binding dimethyl (2R)-5-(3-fluorophenyl)-1H-pyrrolo[1,2-c][1,3]thiazole-6,7-dicarboxylate 2-oxide: P17 (= P17), Y18 (≠ C18), W54 (= W55), M81 (≠ I82), G83 (≠ A84), Y147 (= Y147), G306 (≠ S310), P307 (≠ H311), T308 (≠ S312), F392 (≠ L400)
- binding pyridoxal-5'-phosphate: G114 (= G115), S115 (= S116), Y147 (= Y147), H148 (= H148), E210 (= E212), D244 (= D246), I246 (= I248), K273 (= K275)
4cxrA Mycobaterium tuberculosis transaminase bioa complexed with 1-(1,3- benzothiazol-2-yl)methanamine (see paper)
33% identity, 97% coverage: 5:424/431 of query aligns to 5:416/425 of 4cxrA
- active site: Y18 (≠ C18), Y147 (= Y147), E210 (= E212), D244 (= D246), A247 (≠ L249), K273 (= K275), Y397 (= Y405)
- binding 1-(1,3-benzothiazol-2-yl)methanamine: Y18 (≠ C18), W54 (= W55), W55 (= W56), A216 (= A218)
- binding pyridoxal-5'-phosphate: G114 (= G115), S115 (= S116), Y147 (= Y147), H148 (= H148), E210 (= E212), D244 (= D246), I246 (= I248), K273 (= K275), P307 (≠ H311), T308 (≠ S312)
6ge8A Crystal structure of mycobacterium tuberculosis bioa
33% identity, 97% coverage: 5:424/431 of query aligns to 6:414/422 of 6ge8A
- binding [(2~{S})-3-(1~{H}-indol-3-yl)-1-[(2~{E})-2-[[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylidene]hydrazinyl]-1-oxidanylidene-propan-2-yl]azanium: Y19 (≠ C18), W58 (= W55), G118 (= G115), S119 (= S116), Y151 (= Y147), H152 (= H148), E208 (= E212), D242 (= D246), I244 (= I248), K271 (= K275), P305 (≠ H311), T306 (≠ S312)
6ge8B Crystal structure of mycobacterium tuberculosis bioa
33% identity, 97% coverage: 5:424/431 of query aligns to 6:412/420 of 6ge8B
- binding [(2~{S})-3-(1~{H}-indol-3-yl)-1-[(2~{E})-2-[[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylidene]hydrazinyl]-1-oxidanylidene-propan-2-yl]azanium: W58 (= W55), G118 (= G115), S119 (= S116), Y151 (= Y147), H152 (= H148), A212 (= A218), D240 (= D246), I242 (= I248), K269 (= K275), P303 (≠ H311), T304 (≠ S312)
- binding magnesium ion: H152 (= H148), G153 (= G149), A158 (= A154), C162 (≠ G158)
P9WQ81 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase; 7,8-diamino-pelargonic acid aminotransferase; DAPA AT; DAPA aminotransferase; 7,8-diaminononanoate synthase; DANS; 7,8-diaminopelargonic acid synthase; DAPAS; Diaminopelargonic acid synthase; EC 2.6.1.62 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
33% identity, 97% coverage: 5:424/431 of query aligns to 12:426/437 of P9WQ81
- Y25 (≠ C18) mutation to A: Does not show detectable activity at 335 nm with SAM, even up to concentrations of 3 mM, and shows approximately 70% reduced activity with high concentrations of DAPA (0.5 mM).
- W64 (= W55) binding
- Y157 (= Y147) binding
- K283 (= K275) modified: N6-(pyridoxal phosphate)lysine
- G316 (≠ S310) binding
5te2A Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with a mechanism-based inhibitor (see paper)
33% identity, 97% coverage: 5:424/431 of query aligns to 5:419/428 of 5te2A
- active site: Y18 (≠ C18), Y150 (= Y147), E213 (= E212), D247 (= D246), A250 (≠ L249), K276 (= K275), Y400 (= Y405)
- binding [5-hydroxy-4-({[6-(3-hydroxypropyl)-4-oxo-1,4-dihydropyridin-3-yl]amino}methyl)-6-methylpyridin-3-yl]methyl dihydrogen phosphate: Y18 (≠ C18), W57 (= W55), G117 (= G115), S118 (= S116), Y150 (= Y147), H151 (= H148), G152 (= G149), D247 (= D246), I249 (= I248), K276 (= K275), G309 (≠ S310), P310 (≠ H311), T311 (≠ S312)
Query Sequence
>WP_013459726.1 NCBI__GCF_000183725.1:WP_013459726.1
MNNLEISARDLNVLWHPCTQMKDHETIPLVPIAKGDGVYLEDFEGNRIIDAISSWWVNLF
GHCNPYINRKIKEQLESLEHVILAGFTHEGIVRLSERLVALSPEGLTRCFYADNGSSAIE
VALKMSYHSHKNRGEERGLFVSLTDSYHGETLGALSVGDVALYKETYEPLLIRSIQTLSP
RDQSIEAALEAAKSFEILLQERGKEIAALIVEPLVQGAGGMKMYHPAFLSETKRLCEEYG
IHFIADEILVGFGRTGSMFACEQAQITPDFLVLSKGLTGGYLPLSVVLTTESVYGSFYCD
YNPVRSFLHSHSYTGNALACAAANATLDIFESENVIENNRKTIAAMAEELKRFKSLPKVK
EVRQCGMIAAIEVEEFEPHERIGLKIHRLCMEQGVLIRPLGSVIYVMTPYVITPDELKSV
FDAIESALGNI
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory