SitesBLAST

L86 (= L91) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P160) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (= D165) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.

Sites not aligning to the query:

306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

1g291 Malk (see paper)
44% identity, 72% coverage: 15:223/291 of query aligns to 14:229/372 of 1g291

query
sites
1g291

G

G

S

E

L

V

N

T

A

A

C

V

F

R

E

E

L

M

T

S

-

L

-

E

I

V

T

K

D

D

G

G

E

E

F

F

L

M

T

I

L

L

F

L

G

G

P

P

S
|
S

G
|
G

A
x
C

G
|
G

K
|
K

T
|
T

L

T

M

L

R

R

M

M

I

I

A

A

G

G

L

L

E

E

Q

E

P

P

E

S

S

R

G

G

I

Q

I

I

E

Y

V

I

D

G

G

D

E

K

V

L

W

V

F

A

D
|
D

S

P

T
x
E

K
|
K

K

G

I

I

N

F

L

V

T

P

P

P

Q
x
K

K
x
D

R

R

S

D

V

I

G

A

F

M

V

V

F

F

Q

Q

D

S

Y

Y

A

A

L

L

F

Y

P

P

T

H

M

M

S

T

V

V

R

Y

E

D

N

N

L

I

L

A

F

F

A

P

A

L

E

K

T

L

A

R

E

K

Q

V

R

P

R

R

S

Q

-

E

-

I

-

D

-

Q

-

R

V

V

D

R

E

E

L

V

I

A

E

E

L

L

V

L

E

G

L

L

S

T

Q

E

L

L

S

L

E

N

R

R

L

K

P

P

A

R

T

E

L

L

S

S

G

G

Q

Q

K

R

Q

Q

R

R

V

V

A

A

L

L

A

G

R

R

A

A

L

I

V

V

R

R

H

K

P

P

K

Q

I

V

L

F

L

L

L

M

D

D

E

E

P

P

L

L

S

S

A

N

L

L

D

D

P

A

T

K

M

L

R

R

Q

V

K

R

L

M

Q

R

D

A

E

E

L

L

S

K

L

K

L

L

H

Q

K

R

R

Q

L

L

G

G

I

V

T

T

L

I

L

Y

V

V

S

T

H

H

D

D

I

Q

S

V

E

E

T

A

V

M

K

T

L

M

S

G

D

D

R

R

M

I

A

A

S

V

I

M

E

N

L

R

G

G

K

V

I

L

I

Q

R

Q

V

V

G

G

D

S

P

P

L

D

E

E

F

V

F

Y

S

D

binding magnesium ion: D69 (= D68), E71 (≠ T70), K72 (= K71), K79 (≠ Q78), D80 (≠ K79)
binding pyrophosphate 2-: S38 (= S37), G39 (= G38), C40 (≠ A39), G41 (= G40), K42 (= K41), T43 (= T42), T44 (= T43)

Sites not aligning to the query:

binding magnesium ion: 292, 293, 359

P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
45% identity, 69% coverage: 21:222/291 of query aligns to 23:223/393 of P9WQI3

query
sites
P9WQI3

F

L

E

N

L

L

T

T

I

I

T

A

D

D

G

G

E

E

F

F

L

L

T

I

L

L

F

V

G

G

P

P

S

S

G

G

A

C

G

G

K

K

T

T

L

T

M

L

R

N

M

M

I

I

A

A

G

G

L

L

E

E

Q

D

P

I

E

S

S

S

G

G

I

E

I

L

E

R

V

I

D

A

G

G

E

E

V

-

W

-

F

-

D

-

S

-

T

-

K

-

K

R

I

V

N

N

-

E

L

K

T

A

P

P

Q

K

K

D

R

R

S

D

V

I

G

A

F

M

V

V

F

F

Q

Q

D

S

Y

Y

A

A

L

L

F

Y

P

P

T

H

M

M

S

T

V

V

R

R

E

Q

N

N

L

I

L

A

F

F

A

P

A

L

E

T

T

L

A

A

E

K

Q

M

R

R

R

K

S

A

-

D

-

I

-

A

-

Q

-

K

V

V

D

S

E

E

L

T

I

A

E

K

L

I

V

L

E

D

L

L

S

T

Q

N

L

L

S

L

E

D

R

R

L

K

P

P

A

S

T

Q

L

L

S

S

G

G

Q

Q

K

R

Q

Q

R

R

V

V

A

A

L

M

A

G

R

R

A

A

L

I

V

V

R

R

H

H

P

P

K

K

I

A

L

F

L

L

L

M

D

D

E

E

P

P

L

L

S

S

A

N

L

L

D

D

P

A

T

K

M

L

R

R

Q

V

K

Q

L

M

Q

R

D

G

E

E

L

I

S

A

L

Q

L

L

H

Q

K

R

R

R

L

L

G

G

I

T

T

T

L

V

L

Y

V

V

S

T

H
|
H

D

D

I

Q

S

T

E

E

T

A

V

M

K

T

L

L

S

G

D

D

R

R

M

V

A

V

S

V

I

M

E

Y

L

G

G

G

K

I

I

A

I

Q

R

Q

V

I

G

G

D

T

P

P

L

E

E

E

F

L

F

Y

H193 (= H192) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.

8hplC Lpqy-sugabc in state 1 (see paper)
44% identity, 70% coverage: 21:223/291 of query aligns to 20:221/384 of 8hplC

query
sites
8hplC

F

F

E

S

L

M

T

T

I

I

T

A

D

D

G

G

E

E

F

F

L

I

T

I

L

L

F

V

G

G

P

P

S

S

G
|
G

A
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

T

M

L

R

N

M

M

I

I

A

A

G

G

L

L

E

E

Q

E

P

I

E

T

S

S

G

G

I

E

I

L

E

R

V

I

D

G

G

G

E

E

V

-

W

-

F

-

D

-

S

-

T

-

K

-

K

R

I

V

N

N

-

E

L

K

T

A

P

P

Q

K

K

D

R

R

S

D

V

I

G

A

F

M

V

V

F

F

Q

Q

D

S

Y

Y

A

A

L

L

F

Y

P

P

T

H

M

M

S

T

V

V

R

R

E

Q

N

N

L

I

L

A

F

F

-

P

-

L

-

T

-

L

-

A

A

K

A

V

E

P

T

K

A

A

E

E

Q

I

R

A

S

K

V

V

D

E

E

E

L

T

I

A

E

K

L

I

V

L

E

D

L

L

S

S

Q

E

L

L

S

L

E

D

R

R

L

K

P

P

A

G

T

Q

L

L

S

S

G

G

Q

Q

K

R

Q

Q

R

R

V

V

A

A

L

M

A

G

R

R

A

A

L

I

V

V

R

R

H

S

P

P

K

K

I

A

L

F

L

L

L

M

D

D

E

Q

P

P

L

L

S

S

A

N

L

L

D

D

P

A

T

K

M

L

R

R

Q

V

K

Q

L

M

Q

R

D

A

E

E

L

I

S

S

L

R

L

L

H

Q

K

D

R

R

L

L

G

G

I

T

T

T

L

V

L

Y

V

V

S

T

H

H

D

D

I

Q

S

T

E

E

T

A

V

M

K

T

L

L

S

G

D

D

R

R

M

V

A

V

S

V

I

M

E

L

L

A

G

G

K

E

I

V

I

Q

R

Q

V

I

G

G

D

T

P

P

L

D

E

E

F

L

F

Y

S

S

binding adenosine-5'-triphosphate: G37 (= G38), C38 (≠ A39), G39 (= G40), K40 (= K41), S41 (≠ T42), T42 (= T43)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12

8hprD Lpqy-sugabc in state 4 (see paper)
44% identity, 70% coverage: 21:223/291 of query aligns to 22:223/362 of 8hprD

query
sites
8hprD

F

F

E

S

L

M

T

T

I

I

T

A

D

D

G

G

E

E

F

F

L

I

T

I

L

L

F

V

G

G

P

P

S
|
S

G

G

A
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

T

M

L

R

N

M

M

I

I

A

A

G

G

L

L

E

E

Q

E

P

I

E

T

S

S

G

G

I

E

I

L

E

R

V

I

D

G

G

G

E

E

V

-

W

-

F

-

D

-

S

-

T

-

K

-

K

R

I

V

N

N

-

E

L

K

T

A

P

P

Q

K

K

D

R

R

S

D

V

I

G

A

F

M

V

V

F

F

Q
|
Q

D

S

Y

Y

A

A

L

L

F

Y

P

P

T

H

M

M

S

T

V

V

R

R

E

Q

N

N

L

I

L

A

F

F

-

P

-

L

-

T

-

L

-

A

A

K

A

V

E

P

T

K

A

A

E

E

Q

I

R

A

S

K

V

V

D

E

E

E

L

T

I

A

E

K

L

I

V

L

E

D

L

L

S

S

Q

E

L

L

S

L

E

D

R
|
R

L

K

P

P

A

G

T
x
Q

L

L

S
|
S

G
|
G

Q

Q

K

R

Q

Q

R

R

V

V

A

A

L

M

A

G

R

R

A

A

L

I

V

V

R

R

H

S

P

P

K

K

I

A

L

F

L

L

L

M

D

D

E
x
Q

P

P

L

L

S

S

A

N

L

L

D

D

P

A

T

K

M

L

R

R

Q

V

K

Q

L

M

Q

R

D

A

E

E

L

I

S

S

L

R

L

L

H

Q

K

D

R

R

L

L

G

G

I

T

T

T

L

V

L

Y

V

V

S

T

H
|
H

D

D

I

Q

S

T

E

E

T

A

V

M

K

T

L

L

S

G

D

D

R

R

M

V

A

V

S

V

I

M

E

L

L

A

G

G

K

E

I

V

I

Q

R

Q

V

I

G

G

D

T

P

P

L

D

E

E

F

L

F

Y

S

S

binding adenosine-5'-triphosphate: S38 (= S37), C40 (≠ A39), G41 (= G40), K42 (= K41), S43 (≠ T42), T44 (= T43), Q82 (= Q87), R129 (= R129), Q133 (≠ T133), S135 (= S135), G136 (= G136), G137 (= G137), Q159 (≠ E159), H192 (= H192)
binding magnesium ion: S43 (≠ T42), Q82 (= Q87)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
43% identity, 75% coverage: 5:222/291 of query aligns to 7:221/374 of 2awnB

query
sites
2awnB

N

N

I

V

T

T

K

K

R

A

L
x
W

D

-

T

-

S

G

E

E

G

V

S

V

L
x
V

N

S

A

K

C

D

F

I

E

N

L

L

T

D

I

I

T

H

D

E

G

G

E

E

F

F

L

V

T

V

L

F

F

V

G

G

P

P

S
|
S

G
|
G

A

C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

M

L

R

R

M

M

I

I

A

A

G

G

L

L

E

E

Q

T

P

I

E

T

S

S

G

G

I

D

I

L

E

-

V

-

D

-

G

-

E

-

V

-

W

-

F

F

D

I

S

G

T

E

K

K

K

R

I

M

N

N

L

D

T

T

P

P

-

P

Q

A

K

E

R

R

S

G

V

V

G

G

F

M

V

V

F

F

Q

Q

D

S

Y

Y

A

A

L

L

F

Y

P

P

T

H

M

L

S

S

V

V

R

A

E

E

N

N

L

M

L

S

F

F

A

G

A

L

E

K

T

L

A

A

E

G

Q

A

R

K

S

E

V

V

-

I

-

N

-

Q

-

R

-

V

D

N

E

Q

L

V

I

A

E

E

L

V

V

L

E

Q

L

L

S

A

Q

H

L

L

S

L

E

D

R

R

L

K

P

P

A

K

T

A

L

L

S

S

G

G

Q

Q

K

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

V

V

R

A

H

E

P

P

K

S

I

V

L

F

L

L

D

D

E

E

P

P

L

L

S

S

A

N

L

L

D

D

P

A

T

A

M

L

R

R

Q

V

K

Q

L

M

Q

R

D

I

E

E

L

I

S

S

L

R

L

L

H

H

K

K

R

R

L

L

G

G

I

R

T

T

T

M

L

I

L

Y

V

V

S

T

H

H

D

D

I

Q

S

V

E

E

T

A

V

M

K

T

L

L

S

A

D

D

R

K

M

I

A

V

S

V

I

L

E

D

L

A

G

G

K

R

I

V

I

A

R

Q

V

V

G

G

D

K

P

P

L

L

E

E

F

L

F

Y

binding adenosine-5'-diphosphate: W12 (≠ L10), V17 (≠ L17), S37 (= S37), G38 (= G38), G40 (= G40), K41 (= K41), S42 (≠ T42), T43 (= T43)

8hprC Lpqy-sugabc in state 4 (see paper)
44% identity, 70% coverage: 21:223/291 of query aligns to 22:223/363 of 8hprC

query
sites
8hprC

F

F

E

S

L

M

T

T

I

I

T

A

D

D

G

G

E

E

F

F

L

I

T

I

L

L

F

V

G

G

P

P

S
|
S

G
|
G

A

C

G
|
G

K
|
K

T
x
S

T

T

L

T

M

L

R

N

M

M

I

I

A

A

G

G

L

L

E

E

Q

E

P

I

E

T

S

S

G

G

I

E

I

L

E

R

V

I

D

G

G

G

E

E

V

-

W

-

F

-

D

-

S

-

T

-

K

-

K

R

I

V

N

N

-

E

L

K

T

A

P

P

Q

K

K

D

R

R

S

D

V

I

G

A

F

M

V

V

F

F

Q
|
Q

D

S

Y

Y

A

A

L

L

F

Y

P

P

T

H

M

M

S

T

V

V

R

R

E

Q

N

N

L

I

L

A

F

F

-

P

-

L

-

T

-

L

-

A

A

K

A

V

E

P

T

K

A

A

E

E

Q

I

R

A

S

K

V

V

D

E

E

E

L

T

I

A

E

K

L

I

V

L

E

D

L

L

S

S

Q

E

L

L

S

L

E

D

R

R

L

K

P

P

A

G

T
x
Q

L

L

S

S

G
|
G

Q
|
Q

K

R

Q

Q

R

R

V

V

A

A

L

M

A

G

R

R

A

A

L

I

V

V

R

R

H

S

P

P

K

K

I

A

L

F

L

L

L

M

D

D

E

Q

P

P

L

L

S

S

A

N

L

L

D

D

P

A

T

K

M

L

R

R

Q

V

K

Q

L

M

Q

R

D

A

E

E

L

I

S

S

L

R

L

L

H

Q

K

D

R

R

L

L

G

G

I

T

T

T

L

V

L

Y

V

V

S

T

H
|
H

D

D

I

Q

S

T

E

E

T

A

V

M

K

T

L

L

S

G

D

D

R

R

M

V

A

V

S

V

I

M

E

L

L

A

G

G

K

E

I

V

I

Q

R

Q

V

I

G

G

D

T

P

P

L

D

E

E

F

L

F

Y

S

S

binding adenosine-5'-triphosphate: S38 (= S37), G39 (= G38), G41 (= G40), K42 (= K41), S43 (≠ T42), Q82 (= Q87), Q133 (≠ T133), G136 (= G136), G137 (= G137), Q138 (= Q138), H192 (= H192)
binding magnesium ion: S43 (≠ T42), Q82 (= Q87)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
43% identity, 75% coverage: 5:222/291 of query aligns to 7:221/371 of 3puyA

query
sites
3puyA

N

N

I

V

T

T

K

K

R

A

L
x
W

D

-

T

-

S

G

E

E

G

V

S

V

L

V

N

S

A

K

C

D

F

I

E

N

L

L

T

D

I

I

T

H

D

E

G

G

E

E

F

F

L

V

T

V

L

F

F

V

G

G

P

P

S
|
S

G
|
G

A
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

M

L

R

R

M

M

I

I

A

A

G

G

L

L

E

E

Q

T

P

I

E

T

S

S

G

G

I

D

I

L

E

-

V

-

D

-

G

-

E

-

V

-

W

-

F

F

D

I

S

G

T

E

K

K

K

R

I

M

N

N

L

D

T

T

P

P

-

P

Q

A

K

E

R

R

S

G

V

V

G

G

F

M

V

V

F

F

Q
|
Q

D

S

Y

Y

A

A

L

L

F

Y

P

P

T

H

M

L

S

S

V

V

R

A

E

E

N

N

L

M

L

S

F

F

A

G

A

L

E

K

T

L

A

A

E

G

Q

A

R

K

S

E

V

V

-

I

-

N

-

Q

-

R

-

V

D

N

E

Q

L

V

I

A

E

E

L

V

V

L

E

Q

L

L

S

A

Q

H

L

L

S

L

E

D

R
|
R

L

K

P

P

A

K

T
x
A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

K

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

V

V

R

A

H

E

P

P

K

S

I

V

L

F

L

L

D

D

E
|
E

P

P

L

L

S

S

A

N

L

L

D

D

P

A

T

A

M

L

R

R

Q

V

K

Q

L

M

Q

R

D

I

E

E

L

I

S

S

L

R

L

L

H

H

K

K

R

R

L

L

G

G

I

R

T

T

T

M

L

I

L

Y

V

V

S

T

H
|
H

D

D

I

Q

S

V

E

E

T

A

V

M

K

T

L

L

S

A

D

D

R

K

M

I

A

V

S

V

I

L

E

D

L

A

G

G

K

R

I

V

I

A

R

Q

V

V

G

G

D

K

P

P

L

L

E

E

F

L

F

Y

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ L10), S37 (= S37), G38 (= G38), C39 (≠ A39), G40 (= G40), K41 (= K41), S42 (≠ T42), T43 (= T43), Q81 (= Q87), R128 (= R129), A132 (≠ T133), S134 (= S135), G136 (= G137), Q137 (= Q138), E158 (= E159), H191 (= H192)
binding magnesium ion: S42 (≠ T42), Q81 (= Q87)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
43% identity, 75% coverage: 5:222/291 of query aligns to 7:221/371 of 3puxA

query
sites
3puxA

N

N

I

V

T

T

K

K

R

A

L
x
W

D

-

T

-

S

G

E

E

G

V

S

V

L

V

N

S

A

K

C

D

F

I

E

N

L

L

T

D

I

I

T

H

D

E

G

G

E

E

F

F

L

V

T

V

L

F

F

V

G

G

P

P

S
|
S

G
|
G

A
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

M

L

R

R

M

M

I

I

A

A

G

G

L

L

E

E

Q

T

P

I

E

T

S

S

G

G

I

D

I

L

E

-

V

-

D

-

G

-

E

-

V

-

W

-

F

F

D

I

S

G

T

E

K

K

K

R

I

M

N

N

L

D

T

T

P

P

-

P

Q

A

K

E

R

R

S

G

V

V

G

G

F

M

V

V

F

F

Q
|
Q

D

S

Y

Y

A

A

L

L

F

Y

P

P

T

H

M

L

S

S

V

V

R

A

E

E

N

N

L

M

L

S

F

F

A

G

A

L

E

K

T

L

A

A

E

G

Q

A

R

K

S

E

V

V

-

I

-

N

-

Q

-

R

-

V

D

N

E

Q

L

V

I

A

E

E

L

V

V

L

E

Q

L

L

S

A

Q

H

L

L

S

L

E

D

R
|
R

L

K

P

P

A

K

T

A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

K

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

V

V

R

A

H

E

P

P

K

S

I

V

L

F

L

L

D

D

E

E

P

P

L

L

S

S

A

N

L

L

D

D

P

A

T

A

M

L

R

R

Q

V

K

Q

L

M

Q

R

D

I

E

E

L

I

S

S

L

R

L

L

H

H

K

K

R

R

L

L

G

G

I

R

T

T

T

M

L

I

L

Y

V

V

S

T

H
|
H

D

D

I

Q

S

V

E

E

T

A

V

M

K

T

L

L

S

A

D

D

R

K

M

I

A

V

S

V

I

L

E

D

L

A

G

G

K

R

I

V

I

A

R

Q

V

V

G

G

D

K

P

P

L

L

E

E

F

L

F

Y

binding adenosine-5'-diphosphate: W12 (≠ L10), G38 (= G38), C39 (≠ A39), G40 (= G40), K41 (= K41), S42 (≠ T42), T43 (= T43), R128 (= R129), S134 (= S135), Q137 (= Q138)
binding beryllium trifluoride ion: S37 (= S37), G38 (= G38), K41 (= K41), Q81 (= Q87), S134 (= S135), G136 (= G137), H191 (= H192)
binding magnesium ion: S42 (≠ T42), Q81 (= Q87)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
43% identity, 75% coverage: 5:222/291 of query aligns to 7:221/371 of 3puwA

query
sites
3puwA

N

N

I

V

T

T

K

K

R

A

L
x
W

D

-

T

-

S

G

E

E

G

V

S

V

L
x
V

N

S

A

K

C

D

F

I

E

N

L

L

T

D

I

I

T

H

D

E

G

G

E

E

F

F

L

V

T

V

L

F

F

V

G

G

P

P

S
|
S

G
|
G

A
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

M

L

R

R

M

M

I

I

A

A

G

G

L

L

E

E

Q

T

P

I

E

T

S

S

G

G

I

D

I

L

E

-

V

-

D

-

G

-

E

-

V

-

W

-

F

F

D

I

S

G

T

E

K

K

K

R

I

M

N

N

L

D

T

T

P

P

-

P

Q

A

K

E

R

R

S

G

V

V

G

G

F

M

V

V

F

F

Q
|
Q

D

S

Y

Y

A

A

L

L

F

Y

P

P

T

H

M

L

S

S

V

V

R

A

E

E

N

N

L

M

L

S

F

F

A

G

A

L

E

K

T

L

A

A

E

G

Q

A

R

K

S

E

V

V

-

I

-

N

-

Q

-

R

-

V

D

N

E

Q

L

V

I

A

E

E

L

V

V

L

E

Q

L

L

S

A

Q

H

L

L

S

L

E

D

R
|
R

L

K

P

P

A

K

T
x
A

L

L

S
|
S

G
|
G

Q
|
Q

K

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

V

V

R

A

H

E

P

P

K

S

I

V

L

F

L

L

D

D

E
|
E

P

P

L

L

S

S

A

N

L

L

D

D

P

A

T

A

M

L

R

R

Q

V

K

Q

L

M

Q

R

D

I

E

E

L

I

S

S

L

R

L

L

H

H

K

K

R

R

L

L

G

G

I

R

T

T

T

M

L

I

L

Y

V

V

S

T

H
|
H

D

D

I

Q

S

V

E

E

T

A

V

M

K

T

L

L

S

A

D

D

R

K

M

I

A

V

S

V

I

L

E

D

L

A

G

G

K

R

I

V

I

A

R

Q

V

V

G

G

D

K

P

P

L

L

E

E

F

L

F

Y

binding adenosine-5'-diphosphate: W12 (≠ L10), V17 (≠ L17), G38 (= G38), C39 (≠ A39), G40 (= G40), K41 (= K41), S42 (≠ T42), T43 (= T43), R128 (= R129), A132 (≠ T133), S134 (= S135), Q137 (= Q138)
binding tetrafluoroaluminate ion: S37 (= S37), G38 (= G38), K41 (= K41), Q81 (= Q87), S134 (= S135), G135 (= G136), G136 (= G137), E158 (= E159), H191 (= H192)
binding magnesium ion: S42 (≠ T42), Q81 (= Q87)

3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
43% identity, 75% coverage: 5:222/291 of query aligns to 7:221/371 of 3puvA

query
sites
3puvA

N

N

I

V

T

T

K

K

R

A

L
x
W

D

-

T

-

S

G

E

E

G

V

S

V

L
x
V

N

S

A

K

C

D

F

I

E

N

L

L

T

D

I

I

T

H

D

E

G

G

E

E

F

F

L

V

T

V

L

F

F

V

G

G

P

P

S

S

G
|
G

A
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

M

L

R

R

M

M

I

I

A

A

G

G

L

L

E

E

Q

T

P

I

E

T

S

S

G

G

I

D

I

L

E

-

V

-

D

-

G

-

E

-

V

-

W

-

F

F

D

I

S

G

T

E

K

K

K

R

I

M

N

N

L

D

T

T

P

P

-

P

Q

A

K

E

R

R

S

G

V

V

G

G

F

M

V

V

F

F

Q
|
Q

D

S

Y

Y

A

A

L

L

F

Y

P

P

T

H

M

L

S

S

V

V

R

A

E

E

N

N

L

M

L

S

F

F

A

G

A

L

E

K

T

L

A

A

E

G

Q

A

R

K

S

E

V

V

-

I

-

N

-

Q

-

R

-

V

D

N

E

Q

L

V

I

A

E

E

L

V

V

L

E

Q

L

L

S

A

Q

H

L

L

S

L

E

D

R
|
R

L

K

P

P

A

K

T
x
A

L

L

S
|
S

G

G

Q
|
Q

K

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

V

V

R

A

H

E

P

P

K

S

I

V

L

F

L

L

D

D

E

E

P

P

L

L

S

S

A

N

L

L

D

D

P

A

T

A

M

L

R

R

Q

V

K

Q

L

M

Q

R

D

I

E

E

L

I

S

S

L

R

L

L

H

H

K

K

R

R

L

L

G

G

I

R

T

T

T

M

L

I

L

Y

V

V

S

T

H

H

D

D

I

Q

S

V

E

E

T

A

V

M

K

T

L

L

S

A

D

D

R

K

M

I

A

V

S

V

I

L

E

D

L

A

G

G

K

R

I

V

I

A

R

Q

V

V

G

G

D

K

P

P

L

L

E

E

F

L

F

Y

binding adenosine-5'-diphosphate: W12 (≠ L10), V17 (≠ L17), G38 (= G38), C39 (≠ A39), G40 (= G40), K41 (= K41), S42 (≠ T42), T43 (= T43), R128 (= R129), A132 (≠ T133), S134 (= S135), Q137 (= Q138)
binding magnesium ion: S42 (≠ T42), Q81 (= Q87)

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
43% identity, 75% coverage: 5:222/291 of query aligns to 8:222/371 of P68187

query
sites
P68187

N

N

I

V

T

T

K

K

R

A

L

W

D

-

T

-

S

G

E

E

G

V

S

V

L

V

N

S

A

K

C

D

F

I

E

N

L

L

T

D

I

I

T

H

D

E

G

G

E

E

F

F

L

V

T

V

L

F

F

V

G

G

P

P

S

S

G

G

A

C

G

G

K

K

T

S

T

T

L

L

M

L

R

R

M

M

I

I

A

A

G

G

L

L

E

E

Q

T

P

I

E

T

S

S

G

G

I

D

I

L

E

-

V

-

D

-

G

-

E

-

V

-

W

-

F

F

D

I

S

G

T

E

K

K

K

R

I

M

N

N

L

D

T

T

P

P

-

P

Q

A

K

E

R

R

S

G

V

V

G

G

F

M

V

V

F

F

Q

Q

D

S

Y

Y

A
|
A

L

L

F

Y

P

P

T

H

M

L

S

S

V

V

R

A

E

E

N

N

L

M

L

S

F

F

A

G

A

L

E

K

T

L

A

A

E

G

Q

A

R
x
K

R

K

S

E

V

V

-

I

-

N

-

Q

-

R

-
x
V

D

N

E

Q

L
x
V

I

A

E
|
E

L

V

V

L

E

Q

L

L

S
x
A

Q

H

L

L

S

L

E

D

R

R

L

K

P

P

A

K

T

A

L

L

S

S

G

G

G
|
G

Q

Q

K

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

V

V

R

A

H

E

P

P

K

S

I

V

L

F

L

L

D
|
D

E

E

P

P

L

L

S

S

A

N

L

L

D

D

P

A

T

A

M

L

R

R

Q

V

K

Q

L

M

Q

R

D

I

E

E

L

I

S

S

L

R

L

L

H

H

K

K

R

R

L

L

G

G

I

R

T

T

T

M

L

I

L

Y

V

V

S

T

H

H

D

D

I

Q

S

V

E

E

T

A

V

M

K

T

L

L

S

A

D

D

R

K

M

I

A

V

S

V

I

L

E

D

L

A

G

G

K

R

I

V

I

A

R

Q

V

V

G

G

D

K

P

P

L

L

E

E

F

L

F

Y

A85 (= A90) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ R111) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (vs. gap) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ L117) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (= E119) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (≠ S124) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G137) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D158) mutation to N: Loss of maltose transport but retains ability to repress mal genes.

Sites not aligning to the query:

228 R→C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
43% identity, 75% coverage: 5:222/291 of query aligns to 5:219/367 of 1q12A

query
sites
1q12A

N

N

I

V

T

T

K

K

R

A

L
x
W

D

-

T

-

S

G

E

E

G

V

S

V

L

V

N

S

A

K

C

D

F

I

E

N

L

L

T

D

I

I

T

H

D

E

G

G

E

E

F

F

L

V

T

V

L

F

F

V

G

G

P

P

S
|
S

G
|
G

A
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

M

L

R

R

M

M

I

I

A

A

G

G

L

L

E

E

Q

T

P

I

E

T

S

S

G

G

I

D

I

L

E

-

V

-

D

-

G

-

E

-

V

-

W

-

F

F

D

I

S

G

T

E

K

K

K

R

I

M

N

N

L

D

T

T

P

P

-

P

Q

A

K

E

R

R

S

G

V

V

G

G

F

M

V

V

F

F

Q

Q

D

S

Y

Y

A

A

L

L

F

Y

P

P

T

H

M

L

S

S

V

V

R

A

E

E

N

N

L

M

L

S

F

F

A

G

A

L

E

K

T

L

A

A

E

G

Q

A

R

K

S

E

V

V

-

I

-

N

-

Q

-

R

-

V

D

N

E

Q

L

V

I

A

E

E

L

V

V

L

E

Q

L

L

S

A

Q

H

L

L

S

L

E

D

R
|
R

L

K

P

P

A

K

T
x
A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

K

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

V

V

R

A

H

E

P

P

K

S

I

V

L

F

L

L

D

D

E

E

P

P

L

L

S

S

A

N

L

L

D

D

P

A

T

A

M

L

R

R

Q

V

K

Q

L

M

Q

R

D

I

E

E

L

I

S

S

L

R

L

L

H

H

K

K

R

R

L

L

G

G

I

R

T

T

T

M

L

I

L

Y

V

V

S

T

H

H

D

D

I

Q

S

V

E

E

T

A

V

M

K

T

L

L

S

A

D

D

R

K

M

I

A

V

S

V

I

L

E

D

L

A

G

G

K

R

I

V

I

A

R

Q

V

V

G

G

D

K

P

P

L

L

E

E

F

L

F

Y

binding adenosine-5'-triphosphate: W10 (≠ L10), S35 (= S37), G36 (= G38), C37 (≠ A39), G38 (= G40), K39 (= K41), S40 (≠ T42), T41 (= T43), R126 (= R129), A130 (≠ T133), S132 (= S135), G134 (= G137), Q135 (= Q138)

P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
46% identity, 75% coverage: 21:237/291 of query aligns to 36:251/378 of P69874

query
sites
P69874

F

L

E

D

L

L

T

T

I

I

T

N

D

N

G

G

E

E

F
|
F

L

L

T

T

L

L

F

L

G

G

P

P

S

S

G

G

A
x
C

G

G

K

K

T

T

L

V

M
x
L

R

R

M

L

I

I

A

A

G

G

L

L

E

E

Q

T

P

V

E

D

S

S

G

G

I

R

I

I

E

M

V
x
L

D

D

G

N

E

E

V

-

W

-

F

-

D

D

S

I

T

T

K

-

I

-

N

H

L

V

T

P

P

A

Q

E

K

N

R

R

S

Y

V

V

G

N

F

T

V

V

F

F

Q

Q

D

S

Y

Y

A

A

L

L

F

F

P

P

T

H

M

M

S

T

V

V

R

F

E

E

N

N

L

V

L

A

F

F

A

G

-

L

-

R

-

M

-

Q

-

K

A

T

E

P

T

A

A

A

E

E

Q

I

R

T

R

P

S

R

V

V

D

M

E

E

L

A

I

L

E

R

L

M

V
|
V

E

Q

L

L

S

E

Q

T

L

F

S

A

E

Q

R

R

L

K

P

P

A

H

T

Q

L

L

S

S

G

G

Q

Q

K

Q

Q

Q

R

R

V

V

A

A

L

I

A

A

R

R

A

A

L

V

V

V

R

N

H

K

P

P

K

R

I

L

L

L

D
|
D

E

E

P

S

L

L

S

S

A

A

L

L

D

D

P

Y

T

K

M

L

R

R

Q

K

K

Q

L

M

Q

Q

D

N

E

E

L

L

S

K

L

A

L

L

H

Q

K

R

R

K

L

L

G

G

I

I

T

T

T

F

L

V

L

F

V

V

S

T

H

H

D

D

I

Q

S

E

E

E

T

A

V

L

K

T

L

M

S

S

D

D

R

R

M

I

A

V

S

V

I

M

E

R

L

D

G

G

K

R

I

I

I

E

R

Q

V

D

G

G

D

T

P

P

L

R

E

E

F

I

F

Y

-

E

S

E

P

P

H

K

T

N

L

L

S

F

T

V

K

A

L

-

Q

G

L

F

I

I

G

G

E

E

V

I

F45 (= F30) mutation to L: Lower ATPase activity and transport efficiency.
C54 (≠ A39) mutation to T: Loss of ATPase activity and transport.
L60 (≠ M45) mutation to F: Lower ATPase activity and transport efficiency.
L76 (≠ V61) mutation to P: Lower ATPase activity and transport efficiency.
V135 (= V121) mutation to M: Loss of ATPase activity and transport.
D172 (= D158) mutation to N: Loss of ATPase activity and transport.

Sites not aligning to the query:

26 C→A: Lower ATPase activity and transport efficiency.
27 F→L: Lower ATPase activity and transport efficiency.
276 C→A: Lower ATPase activity and transport efficiency.
297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.

8y5iA Cryo-em structure of e.Coli spermidine transporter potd-potabc in translocation intermidiate state (see paper)
46% identity, 74% coverage: 22:237/291 of query aligns to 22:236/358 of 8y5iA

query
sites
8y5iA

E

D

L

L

T

T

I

I

T

N

D

N

G

G

E

E

F

F

L

L

T

T

L

L

F

L

G

G

P

P

S

S

G
|
G

A

C

G

G

K
|
K

T
|
T

L

V

M

L

R

R

M

L

I

I

A

A

G

G

L

L

E

E

Q

T

P

V

E

D

S

S

G

G

I

R

I

I

E

M

V

L

D

D

G

N

E

E

V

-

W

-

F

-

D

D

S

I

T

T

K

-

I

-

N

H

L

V

T

P

P

A

Q

E

K

N

R

R

S

Y

V

V

G

N

F

T

V

V

F

F

Q

Q

D

S

Y

Y

A

A

L

L

F

F

P

P

T

H

M

M

S

T

V

V

R

F

E

E

N

N

L

V

L

A

F

F

A

G

-

L

-

R

-

M

-

Q

-

K

A

T

E

P

T

A

A

A

E

E

Q

I

R

T

R

P

S

R

V

V

D

M

E

E

L

A

I

L

E

R

L

M

V

V

E

Q

L

L

S

E

Q

T

L

F

S

A

E

Q

R
|
R

L

K

P

P

A

H

T
x
Q

L

L

S
|
S

G

G

Q

Q

K

Q

Q

Q

R

R

V

V

A

A

L

I

A

A

R

R

A

A

L

V

V

V

R

N

H

K

P

P

K

R

I

L

L

L

D

D

E

Q

P

S

L

L

S

S

A

A

L

L

D

D

P

Y

T

K

M

L

R

R

Q

K

K

Q

L

M

Q

Q

D

N

E

E

L

L

S

K

L

A

L

L

H

Q

K

R

R

K

L

L

G

G

I

I

T

T

T

F

L

V

L

F

V

V

S

T

H

H

D

D

I

Q

S

E

E

E

T

A

V

L

K

T

L

M

S

S

D

D

R

R

M

I

A

V

S

V

I

M

E

R

L

D

G

G

K

R

I

I

I

E

R

Q

V

D

G

G

D

T

P

P

L

R

E

E

F

I

F

Y

-

E

S

E

P

P

H

K

T

N

L

L

S

F

T

V

K

A

L

-

Q

G

L

F

I

I

G

G

E

E

V

I

binding adenosine-5'-triphosphate: G38 (= G38), K41 (= K41), T42 (= T42), T43 (= T43), R128 (= R129), Q132 (≠ T133), S134 (= S135)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12, 15

3d31A Modbc from methanosarcina acetivorans (see paper)
46% identity, 69% coverage: 23:222/291 of query aligns to 21:216/348 of 3d31A

query
sites
3d31A

L

L

T

K

I

V

T

E

D

S

G

G

E

E

F

Y

L

F

T

V

L

I

F

L

G

G

P

P

S

T

G

G

A

A

G

G

K

K

T

T

T

L

L

F

M

L

R

E

M

L

I

I

A

A

G

G

L

F

E

H

Q

V

P

P

E

D

S

S

G

G

I

R

I

I

E

L

V

L

D

D

G

G

E

K

V

-

W

-

F

-

D

D

S

V

T

T

K

-

I

-

N

D

L

L

T

S

P

P

Q

E

K

K

R

H

S

D

V

I

G

A

F

F

V

V

F

Y

Q

Q

D

N

Y

Y

A

S

L

L

F

F

P

P

T

H

M

M

S

N

V

V

R

K

E

K

N

N

L

L

L

E

F

F

A

G

-

M

-

R

-

M

A

K

E

K

T

I

A

K

E

D

Q

P

R

K

R

R

S

V

V

L

D

D

E

T

L

A

I

R

E

D

L

L

V

-

E

K

L

I

S

E

Q

H

L

L

S

L

E

D

R

R

L

N

P

P

A

L

T

T

L

L

S

S

G

G

Q

E

K

Q

Q

Q

R

R

V

V

A

A

L

L

A

A

R

R

A

A

L

L

V

V

R

T

H

N

P

P

K

K

I

I

L

L

D

D

E

E

P

P

L

L

S

S

A

A

L

L

D

D

P

P

T

R

M

T

R

Q

Q

E

K

N

L

A

Q

R

D

E

E

M

L

L

S

S

L

V

L

L

H

H

K

K

R

K

L

N

G

K

I

L

T

T

T

V

L

L

L

H

V

I

S

T

H

H

D

D

I

Q

S

T

E

E

T

A

V

R

K

I

L

M

S

A

D

D

R

R

M

I

A

A

S

V

I

V

E

M

L

D

G

G

K

K

I

L

I

I

R

Q

V

V

G

G

D

K

P

P

L

E

E

E

F

I

F

F

Sites not aligning to the query:

binding tungstate(vi)ion: 286, 320, 323, 340, 341, 342

1oxvD Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
39% identity, 76% coverage: 22:241/291 of query aligns to 25:248/353 of 1oxvD

query
sites
1oxvD

E

N

L

I

T

N

I

I

T

E

D

N

G

G

E

E

F

R

L

F

T

G

L

I

F

L

G

G

P

P

S
|
S

G
|
G

A

A

G
|
G

K
|
K

T
|
T

L

F

M

M

R

R

M

I

I

I

A

A

G

G

L

L

E

D

Q

V

P

P

E

S

S

T

G

G

I

-

E

-

V

-

D

-

G

-

E

E

V

L

W

Y

F

F

D

D

-

D

-

R

-

L

-

V

-

A

S

S

T

N

K

G

K

K

I

L

N

I

L

V

T

P

P

P

Q

E

K

D

R

R

S

K

V

I

G

G

F

M

V

V

F

F

Q
|
Q

D

T

Y

W

A

A

L

L

F

Y

P

P

T

N

M

L

S

T

V

A

R

F

E

E

N

N

L

I

L

A

F

F

-

P

-

L

-

T

-

N

-

M

A

K

A

M

E

S

T

K

A

E

E

E

Q

I

R

R

R

K

S

R

V

V

D

E

E

E

L

V

I

A

E

K

L

I

V

L

E

D

L

I

S

H

Q

H

L

V

S

L

E

N

R

H

L

F

P

P

A

R

T

E

L

L

S

S

G

G

Q

Q

K

Q

Q

Q

R

R

V

V

A

A

L

L

A

A

R

R

A

A

L

L

V

V

R

K

H

D

P

P

K

S

I

L

L

L

D

D

E
|
E

P

P

L

F

S

S

A

N

L

L

D

D

P

A

T

R

M

M

R

R

Q

D

K

S

L

A

Q

R

D

A

E

L

L

V

S

K

L

E

L

V

H

Q

K

S

R

R

L

L

G

G

I

V

T

T

T

L

L

L

L

V

V

V

S

S

H

H

D

D

I

P

S

A

E

D

T

I

V

F

K

A

L

I

S

A

D

D

R

R

M

V

A

G

S

V

I

L

E

V

L

K

G

G

K

K

I

L

I

V

R

Q

V

V

G

G

D

K

P

P

L

E

E

D

F

L

F

Y

S

D

P

N

H

P

T

V

L

S

S

I

T

Q

K

V

L

A

Q

S

L

L

I

I

G

G

E

E

V

I

L

N

K

E

I

L

E

E

binding phosphoaminophosphonic acid-adenylate ester: S40 (= S37), G41 (= G38), G43 (= G40), K44 (= K41), T45 (= T42), T46 (= T43), Q89 (= Q87), E166 (= E159)
binding magnesium ion: T45 (= T42), Q89 (= Q87)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 13

1oxvA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
39% identity, 76% coverage: 22:241/291 of query aligns to 25:248/353 of 1oxvA

query
sites
1oxvA

E

N

L

I

T

N

I

I

T

E

D

N

G

G

E

E

F

R

L

F

T

G

L

I

F

L

G

G

P

P

S
|
S

G
|
G

A

A

G
|
G

K
|
K

T
|
T

L

F

M

M

R

R

M

I

I

I

A

A

G

G

L

L

E

D

Q

V

P

P

E

S

S

T

G

G

I

-

E

-

V

-

D

-

G

-

E

E

V

L

W

Y

F

F

D

D

-

D

-

R

-

L

-

V

-

A

S

S

T

N

K

G

K

K

I

L

N

I

L

V

T

P

P

P

Q

E

K

D

R

R

S

K

V

I

G

G

F

M

V

V

F

F

Q

Q

D

T

Y

W

A

A

L

L

F

Y

P

P

T

N

M

L

S

T

V

A

R

F

E

E

N

N

L

I

L

A

F

F

-

P

-

L

-

T

-

N

-

M

A

K

A

M

E

S

T

K

A

E

E

E

Q

I

R

R

R

K

S

R

V

V

D

E

E

E

L

V

I

A

E

K

L

I

V

L

E

D

L

I

S

H

Q

H

L

V

S

L

E

N

R

H

L

F

P

P

A

R

T

E

L

L

S

S

G

G

Q

Q

K

Q

Q

Q

R

R

V

V

A

A

L

L

A

A

R

R

A

A

L

L

V

V

R

K

H

D

P

P

K

S

I

L

L

L

D

D

E
|
E

P

P

L

F

S

S

A

N

L

L

D

D

P

A

T

R

M

M

R

R

Q

D

K

S

L

A

Q

R

D

A

E

L

L

V

S

K

L

E

L

V

H

Q

K

S

R

R

L

L

G

G

I

V

T

T

T

L

L

L

L

V

V

V

S

S

H

H

D

D

I

P

S

A

E

D

T

I

V

F

K

A

L

I

S

A

D

D

R

R

M

V

A

G

S

V

I

L

E

V

L

K

G

G

K

K

I

L

I

V

R

Q

V

V

G

G

D

K

P

P

L

E

E

D

F

L

F

Y

S

D

P

N

H

P

T

V

L

S

S

I

T

Q

K

V

L

A

Q

S

L

L

I

I

G

G

E

E

V

I

L

N

K

E

I

L

E

E

binding phosphoaminophosphonic acid-adenylate ester: S40 (= S37), G41 (= G38), G43 (= G40), K44 (= K41), T45 (= T42), T46 (= T43), E166 (= E159)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 13

1oxuA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
39% identity, 76% coverage: 22:241/291 of query aligns to 25:248/353 of 1oxuA

query
sites
1oxuA

E

N

L

I

T

N

I

I

T

E

D

N

G

G

E

E

F

R

L

F

T

G

L

I

F

L

G

G

P

P

S

S

G
|
G

A

A

G
|
G

K
|
K

T
|
T

L

F

M

M

R

R

M

I

I

I

A

A

G

G

L

L

E

D

Q

V

P

P

E

S

S

T

G

G

I

-

E

-

V

-

D

-

G

-

E

E

V

L

W

Y

F

F

D

D

-

D

-

R

-

L

-

V

-

A

S

S

T

N

K

G

K

K

I

L

N

I

L

V

T

P

P

P

Q

E

K

D

R

R

S

K

V

I

G

G

F

M

V

V

F

F

Q

Q

D

T

Y

W

A

A

L

L

F

Y

P

P

T

N

M

L

S

T

V

A

R

F

E

E

N

N

L

I

L

A

F

F

-

P

-

L

-

T

-

N

-

M

A

K

A

M

E

S

T

K

A

E

E

E

Q

I

R

R

R

K

S

R

V

V

D

E

E

E

L

V

I

A

E

K

L

I

V

L

E

D

L

I

S

H

Q

H

L

V

S

L

E

N

R

H

L

F

P

P

A

R

T

E

L

L

S

S

G

G

Q

Q

K

Q

Q

Q

R

R

V

V

A

A

L

L

A

A

R

R

A

A

L

L

V

V

R

K

H

D

P

P

K

S

I

L

L

L

D

D

E

E

P

P

L

F

S

S

A

N

L

L

D

D

P

A

T

R

M

M

R

R

Q

D

K

S

L

A

Q

R

D

A

E

L

L

V

S

K

L

E

L

V

H

Q

K

S

R

R

L

L

G

G

I

V

T

T

T

L

L

L

L

V

V

V

S

S

H

H

D

D

I

P

S

A

E

D

T

I

V

F

K

A

L

I

S

A

D

D

R

R

M

V

A

G

S

V

I

L

E

V

L

K

G

G

K

K

I

L

I

V

R

Q

V

V

G

G

D

K

P

P

L

E

E

D

F

L

F

Y

S

D

P

N

H

P

T

V

L

S

S

I

T

Q

K

V

L

A

Q

S

L

L

I

I

G

G

E

E

V

I

L

N

K

E

I

L

E

E

binding adenosine-5'-diphosphate: G41 (= G38), G43 (= G40), K44 (= K41), T45 (= T42), T46 (= T43)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 13, 20

Query Sequence

>WP_013459998.1 NCBI__GCF_000183725.1:WP_013459998.1
MISVNITKRLDTSEGSLNACFELTITDGEFLTLFGPSGAGKTTLMRMIAGLEQPESGIIE
VDGEVWFDSTKKINLTPQKRSVGFVFQDYALFPTMSVRENLLFAAETAEQRRSVDELIEL
VELSQLSERLPATLSGGQKQRVALARALVRHPKILLLDEPLSALDPTMRQKLQDELSLLH
KRLGITTLLVSHDISETVKLSDRMASIELGKIIRVGDPLEFFSPHTLSTKLQLIGEVLKI
EEDFPISVVTLLVGSSVVRTVLSNEEAHLLCVGKHAIISTKAFNPIVMPVE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory