SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013460285.1 NCBI__GCF_000183725.1:WP_013460285.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

6addA The crystal structure of rv2747 from mycobacterium tuberculosis in complex with coa and nlq (see paper)
34% identity, 72% coverage: 12:121/153 of query aligns to 13:124/168 of 6addA

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binding coenzyme a: Y24 (≠ E25), I28 (= I29), V75 (≠ M72), A76 (≠ I73), V77 (≠ I74), T82 (≠ R79), G83 (= G80), H84 (≠ R81), G85 (≠ N82), G87 (= G84), H88 (≠ T85), T110 (= T107), E112 (≠ Q109), F115 (= F112)
binding n~2~-acetyl-l-glutamine: K27 (≠ V28), I28 (= I29), L30 (≠ P31), T74 (≠ S71), L109 (= L106)

Sites not aligning to the query:

5yo2A The crystal structure of rv2747 from mycobacterium tuberculosis in complex with acetyl coa and l-arginine (see paper)
34% identity, 72% coverage: 12:121/153 of query aligns to 13:124/168 of 5yo2A

query
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binding acetyl coenzyme *a: Y24 (≠ E25), I28 (= I29), I72 (≠ L69), R73 (= R70), T74 (≠ S71), V75 (≠ M72), V77 (≠ I74), T82 (≠ R79), G83 (= G80), H84 (≠ R81), G85 (≠ N82), G87 (= G84), H88 (≠ T85), T110 (= T107), E112 (≠ Q109), F115 (= F112), F116 (= F113)
binding arginine: G26 (= G27), L29 (= L30), L30 (≠ P31), R73 (= R70), L109 (= L106)

Sites not aligning to the query:

binding arginine: 147

5ygeA Arga complexed with acecoa and glutamate (see paper)
34% identity, 72% coverage: 12:121/153 of query aligns to 14:125/169 of 5ygeA

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binding acetyl coenzyme *a: Y25 (≠ E25), I73 (≠ L69), V76 (≠ M72), A77 (≠ I73), V78 (≠ I74), T83 (≠ R79), G84 (= G80), H85 (≠ R81), G86 (≠ N82), G88 (= G84), H89 (≠ T85), T111 (= T107), E113 (≠ Q109), F117 (= F113)
binding glutamic acid: K33 (≠ S33), E44 (≠ S43), H64 (= H60), R74 (= R70)

Sites not aligning to the query:

binding acetyl coenzyme *a: 1, 2

Query Sequence

>WP_013460285.1 NCBI__GCF_000183725.1:WP_013460285.1
MNITYKKPTLLDIPAMQQLVAPEIESGVILPRSNDEIATNIRSYFLALDGEKLVGFVALH
IHSPALAELRSMIIDTAYRGRNIGTTLVENACEEGRRLGLKEVLALTYQQRFFERLGFVE
IPKESIPEHKIWADCIKCKHFPVCNEVSLIKTL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory