SitesBLAST

G98 (= G12) binding NAD(+)
F99 (= F13) binding NAD(+)
V100 (= V14) binding NAD(+)
D119 (= D33) binding NAD(+)
N120 (= N34) binding NAD(+)
F122 (= F36) binding NAD(+)
T123 (= T37) binding NAD(+)
G124 (= G38) binding NAD(+)
D144 (= D58) binding NAD(+)
V145 (= V59) binding NAD(+)
L149 (≠ I63) binding UDP-alpha-D-glucuronate
Y150 (≠ L64) binding UDP-alpha-D-glucuronate
L159 (= L73) binding NAD(+)
S161 (≠ C75) binding NAD(+)
K177 (≠ R91) binding UDP-alpha-D-glucuronate
T178 (= T92) binding NAD(+)
N185 (= N99) binding UDP-alpha-D-glucuronate
G188 (≠ D102) binding UDP-alpha-D-glucuronate
K191 (≠ R105) binding UDP-alpha-D-glucuronate
R192 (≠ K106) binding UDP-alpha-D-glucuronate
A200 (= A114) binding NAD(+)
E204 (= E118) mutation to A: Reduced UDP-glucuronic acid decarboxylase activity.
Y231 (= Y145) active site, Proton acceptor; binding NAD(+); mutation to F: Abolished UDP-glucuronic acid decarboxylase activity.
K235 (= K149) binding NAD(+)
R236 (= R150) mutation to H: Strongly reduced UDP-glucuronic acid decarboxylase activity, caused by a local unfolding of the active site that allows for a rotation of the dimer interface, leading to the formation of a homohexamer.
Y245 (= Y159) binding UDP-alpha-D-glucuronate
Q248 (= Q162) binding UDP-alpha-D-glucuronate
E249 (≠ H163) binding UDP-alpha-D-glucuronate
T261 (= T175) binding NAD(+)
H267 (≠ N181) binding NAD(+)
R272 (= R186) binding NAD(+)
R361 (= R277) mutation to Q: Strongly reduced UDP-glucuronic acid decarboxylase activity.

Sites not aligning to the query:

1 modified: N-acetylmethionine

2b69A Crystal structure of human udp-glucoronic acid decarboxylase
58% identity, 98% coverage: 2:308/312 of query aligns to 1:305/312 of 2b69A

query
sites
2b69A

R

R

K

K

R

R

I

I

L

L

V

I

T

T

G
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G

G

G

A

A

G
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G

F
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F

V
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V

G

G

S

S

H

H

L

L

C

T

R

D

R

K

L

L

L

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D

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N

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G

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K

E

R

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N

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C
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Y

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F

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M

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G

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N

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F

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G

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M

N

H

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M

D

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D

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R

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N

F

F

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A

A

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T

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Y

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Q

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R

S

A

F

F

Q

Q

Y

Y

V

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D

S

D

D

L

L

V

V

E

N

G

G

M

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M

N

N

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N

D

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F

-

T

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G

S

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N

N

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G

N

N

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G

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x
I

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L

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active site: T115 (= T116), S116 (= S117), E117 (= E118), Y144 (= Y145), K148 (= K149), R185 (= R186)
binding nicotinamide-adenine-dinucleotide: G8 (= G9), G11 (= G12), F12 (= F13), V13 (= V14), D32 (= D33), N33 (= N34), T36 (= T37), G37 (= G38), D57 (= D58), V58 (= V59), L72 (= L73), A73 (= A74), S74 (≠ C75), A76 (= A77), T91 (= T92), T115 (= T116), Y144 (= Y145), K148 (= K149), I171 (= I172), N173 (= N174), R185 (= R186)
binding uridine-5'-diphosphate: P61 (= P62), L62 (≠ I63), Y63 (≠ L64), P78 (= P79), N98 (= N99), G101 (≠ D102), L102 (≠ I103), K104 (≠ R105), R105 (≠ K106), Y158 (= Y159), N173 (= N174), R185 (= R186), V186 (= V187), N189 (= N190), T201 (= T202), Y203 (= Y204), Q208 (= Q209), R210 (= R211), I244 (≠ M247), D270 (= D273)

Q6GMI9 UDP-glucuronic acid decarboxylase 1; UDP-glucuronate decarboxylase 1; UXS-1; EC 4.1.1.35 from Danio rerio (Zebrafish) (Brachydanio rerio) (see paper)
58% identity, 98% coverage: 2:307/312 of query aligns to 86:389/418 of Q6GMI9

query
sites
Q6GMI9

R

R

K

K

R

R

I

I

L

L

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I

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T

G

G

A

A

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G

F

F

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H

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D

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N

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M

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N

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A

A

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G

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A

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Y

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A

F

F

Q

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Y

Y

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D

D

L

L

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N

G

G

M

L

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S

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L

M

M

N

N

S

S

D

N

D

-

S

-

F

I

T

S

G

S

P

P

V

V

N

N

I

L

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G

N

N

P

P

G

E

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M

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A

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F

R234 (= R150) mutation to H: In Man o' war (mow) mutant; characterized by severe craniofacial defects. Fishes show defective organization and morphogenesis of chondrocytes, perichondrium and bone.

4lk3B Crystal structure of human udp-xylose synthase r236a substitution (see paper)
50% identity, 98% coverage: 2:308/312 of query aligns to 1:267/274 of 4lk3B

query
sites
4lk3B

R

R

K

K

R

R

I

I

L

L

V

I

T

T

G
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G

G

G

A

A

G
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G

F
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F

V
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V

G

G

S

S

H

H

L

L

C

T

R

D

R

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L

L

M

N

M

E

D

G

G

N

H

D

E

V

V

I

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C

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L

V

D
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D

N
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N

F

F

T
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T

G
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G

S

R

K

K

E

R

N

N

I

V

I

E

D

H

L

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L

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G

H

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P

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F

E

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H

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D

V
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V

Q

V

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E

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P

I
x
L

L
x
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L

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E

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D

E

Q

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Y

N

H

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L

A
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A

C
x
S

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P

A

A

S

S

P

P

P

-

H

-

Y

-

Q

M

F

Y

D

N

P

P

V
x
I

A

K

T

T

T

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x
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E

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I

F

F

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N

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M

N
x
H

P

M

D

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D

D

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G

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R

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V

V

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S

S

N
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N

F

F

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Q

A

A

L

L

R

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Q

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T

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Y
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Y

G

G

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S

G

G

T

S

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Q

T

T

R
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R

S

A

F

F

Q

Q

Y

Y

V

V

D

S

D

D

L

L

V

V

E

N

G

G

M

L

I

V

R

A

L

L

M

M

N

N

S

S

D

N

D

V

S

S

F

-

T

-

G

S

P

P

V

V

N

N

I

L

G

G

N

N

P

P

G

E

E

E

F

H

T

T

M
x
I

L

L

E

E

L

F

A

A

Q

Q

K

L

I

I

I

K

T

N

L

L

T

V

G

G

S

S

S

G

S

S

K

E

L

I

T

Q

Y

F

K

-

P

-

L

-

P

-

Q

-

D

-

P

-

L

-

Q

L

R

R

Q

K

P

P

D

D

I

I

S

K

L

K

A

A

K

K

K

L

E

M

L

L

D

G

W

W

E

E

P

P

T

V

I

V

A

P

L

L

E

E

G

G

L

L

K

N

K

K

T

A

I

I

A

H

Y

Y

F

F

K

R

active site: T112 (= T116), S113 (= S117), E114 (= E118), K119 (= K149), R156 (= R186)
binding nicotinamide-adenine-dinucleotide: G8 (= G9), G11 (= G12), F12 (= F13), V13 (= V14), D32 (= D33), N33 (= N34), T36 (= T37), G37 (= G38), D57 (= D58), V58 (= V59), L72 (= L73), A73 (= A74), S74 (≠ C75), P75 (= P76), T88 (= T92), A110 (= A114), T112 (= T116), K119 (= K149), I142 (= I172), H151 (≠ N181)
binding uridine-5'-diphosphate: R156 (= R186), V157 (= V187), N160 (= N190), T172 (= T202), Y174 (= Y204), Q179 (= Q209), R181 (= R211), I215 (≠ M247)
binding uridine-5'-diphosphate-glucuronic acid: P61 (= P62), L62 (≠ I63), Y63 (≠ L64), I83 (≠ V87), K87 (≠ R91), N95 (= N99), G98 (≠ D102), L99 (≠ I103), K101 (≠ R105), Y129 (= Y159), E133 (≠ H163)

4lk3C Crystal structure of human udp-xylose synthase r236a substitution (see paper)
49% identity, 98% coverage: 2:308/312 of query aligns to 1:264/271 of 4lk3C

query
sites
4lk3C

R

R

K

K

R

R

I

I

L

L

V

I

T

T

G
|
G

G

G

A

A

G
|
G

F
|
F

V
|
V

G

G

S

S

H

H

L

L

C

T

R

D

R

K

L

L

L

M

N

M

E

D

G

G

N

H

D

E

V

V

I

T

C

V

L

V

D
|
D

N
|
N

F

F

T
|
T

G
|
G

S

R

K

K

E

R

N

N

I

V

I

E

D

H

L

W

L

I

P

G

H

H

P

E

Y

N

F

F

E

E

L

L

I

I

R

N

H

H

D
|
D

V
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V

Q

V

E

E

P
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P

I
x
L

L
x
Y

L

I

E

E

V

V

D

D

E

Q

I

I

Y

Y

N

H

L
|
L

A
|
A

C
x
S

P
|
P

A

A

S

S

P

-

H

-

Y

-

Q

-

F

Y

D

N

P

P

V

I

A

K

T

T

T

L

R

K

T
|
T

S

N

V

T

L

I

G

G

A

T

I

L

N
|
N

M

M

L

L

D
x
G

I
x
L

A

A

R
x
K

K
x
R

C

V

K

G

A

A

K

R

I

L

L

L

Q

L

A

A

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T
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T

S
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S

E
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E

V

V

Y

Y

G

-

D

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P

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E

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H

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Y

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G

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S

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T

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G

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I

-

R

-

A

-

C

-

Y

-

D

-

E

E

G

G

K
|
K

R

A

C

V

A

A

E

E

T

T

L

M

F

C

F

Y

D

A

Y
|
Y

Y

M

R

K

Q

Q

H
x
E

G

G

V

V

K

E

I

V

K

R

V

V

I

A

R

R

I
|
I

F

F

N

N

T

T

Y

F

G

G

P

P

Y

R

M

M

N
x
H

P

M

D

N

D

D

G

G

R
|
R

V
|
V

V

V

S

S

N

N

F

F

I

I

M

L

Q

Q

A

A

L

L

R

Q

N

G

Q

E

D

P

I

L

T

T

I

V

Y

Y

G

G

A

S

G

G

T

S

Q

Q

T

T

R

R

S

A

F

F

Q

Q

Y

Y

V

V

D

S

D

D

L

L

V

V

E

N

G

G

M

L

I

V

R

A

L

L

M

M

N

N

S

S

D

N

D

V

S

S

F

-

T

-

G

S

P

P

V

V

N

N

I

L

G

G

N

N

P

P

G

E

E

E

F

H

T

T

M

I

L

L

E

E

L

F

A

A

Q

Q

K

L

I

I

I

K

T

N

L

L

T

V

G

G

S

S

S

G

S

S

K

E

L

I

T

Q

Y

F

K

-

P

-

L

-

P

-

Q

-

D

-

P

-

L

-

Q

-

R

L

Q

K

P

P

D

D

I

I

S

K

L

K

A

A

K

K

K

L

E

M

L

L

D

G

W

W

E

E

P

P

T

V

I

V

A

P

L

L

E

E

G

G

L

L

K

N

K

K

T

A

I

I

A

H

Y

Y

F

F

K

R

active site: T110 (= T116), S111 (= S117), E112 (= E118), K117 (= K149), R154 (= R186)
binding nicotinamide-adenine-dinucleotide: G8 (= G9), G11 (= G12), F12 (= F13), V13 (= V14), D32 (= D33), N33 (= N34), T36 (= T37), G37 (= G38), D57 (= D58), V58 (= V59), L72 (= L73), A73 (= A74), S74 (≠ C75), P75 (= P76), T86 (= T92), K117 (= K149), I140 (= I172), H149 (≠ N181)
binding pyrophosphate 2-: R154 (= R186), V155 (= V187)
binding uridine-5'-diphosphate-glucuronic acid: P61 (= P62), L62 (≠ I63), Y63 (≠ L64), N93 (= N99), G96 (≠ D102), L97 (≠ I103), K99 (≠ R105), R100 (≠ K106), Y127 (= Y159), E131 (≠ H163)

4m55E Crystal structure of human udp-xylose synthase r236h substitution (see paper)
36% identity, 76% coverage: 4:239/312 of query aligns to 1:156/164 of 4m55E

query
sites
4m55E

R

R

I

I

L

L

V

I

T

T

G
|
G

G

G

A

A

G
|
G

F
|
F

V
|
V

G

G

S

S

H

H

L

L

C

T

R

D

R

K

L

L

L

M

N

M

E

D

G

G

N

H

D

E

V

V

I

T

C

V

L

V

D
|
D

N
|
N

F
|
F

F

F

T
|
T

G
|
G

S

R

K

K

E

R

N

N

I

V

I

E

D

H

L

W

L

I

P

G

H

H

P

E

Y

N

F

F

E

E

L

L

I

I

R

N

H

H

D
|
D

V
|
V

Q

V

E

E

P

P

I

L

L

Y

L

I

E

E

V

V

D

D

E

Q

I

I

Y

Y

N

H

L

L

A

A

C
x
S

P
|
P

A

-

S

-

P

-

H

-

Y

-

Q

-

F

-

D

N

P

P

V

I

A

K

T

T

T

L

R

K

T
|
T

S

N

V

T

L

I

G

G

A

T

I

L

N

N

M

M

L

L

D

G

I

L

A

A

R

K

K

R

C

V

K

G

A

A

K

R

I

L

L

L

Q

L

A

A

S

S

T
|
T

S

S

E

-

V

-

Y

-

G

-

D

-

P

-

E

-

V

-

H

-

P

-

Q

-

P

-

E

-

S

-

Y

-

R

-

G

-

S

-

V

-

S

-

T

-

G

-

I

-

R

-

A

-

C

-

Y

-

D

-

E

-

G

-

K

-

R

-

C

V

A

A

E

E

T

T

L

M

F

C

F

Y

D

A

Y

Y

Y

M

R

K

Q

Q

H

E

G

G

V

V

K

E

I

V

K

R

V

V

I

A

R

R

I

I

F

F

N

N

T
|
T

Y

-

G

-

P

-

Y

-

M

-

N

-

P

-

D

-

G

-

R

-

V

-

S

-

N

-

F

-

I

-

M

-

Q

-

A

-

L

-

R

-

N

-

Q

-

D

-

I

-

T

-

I

-

Y

-

G

-

A

-

G

-

T

-

Q

-

T

-

R

-

S

-

F

F

Q

Q

Y

Y

V

V

D

S

D

D

L

L

V

V

E

N

G

G

M

L

I

V

R

A

L

L

M

M

N

N

S

S

D

N

D

V

S

S

F

-

T

-

G

S

P

P

V

V

N

N

I

L

active site: T105 (= T116)
binding nicotinamide-adenine-dinucleotide: G6 (= G9), G9 (= G12), F10 (= F13), V11 (= V14), D30 (= D33), N31 (= N34), F32 (= F35), T34 (= T37), G35 (= G38), D55 (= D58), V56 (= V59), S72 (≠ C75), P73 (= P76), T81 (= T92), T105 (= T116), T131 (= T175)

4zrnA Crystal structure of udp-glucose 4-epimerase (tm0509) with udp-glucose from hyperthermophilic eubacterium thermotoga maritima (see paper)
32% identity, 97% coverage: 5:308/312 of query aligns to 3:305/309 of 4zrnA

query
sites
4zrnA

I

I

L

L

V

V

T

T

G
|
G

G

G

A

A

G
|
G

F
|
F

V
x
I

G

G

S

S

H

H

L

V

C

V

R

D

R

K

L

L

L

I

N

E

E

N

G

G

N

Y

D

G

V

V

I

I

C

V

L

V

D
|
D

N
|
N

F

L

F
x
S

T
x
S

G
|
G

S

K

K

V

E

E

N

N

I

L

I

N

D

R

L

N

L

A

P

L

H

F

P

Y

Y

E

F

Q

E
x
S

L
x
I

I

E

R

D

H

E

D

E

V

M

Q

M

E

E

P

R

I

I

-

F

-

S

L

L

L

H

E

R

V

P

D

E

E

Y

I

V

Y

F

N

H

L
|
L

A
|
A

C
x
A

P

Q

A

A

S

S

P

V

P

A

H

I

Y

S

Q

V

F

R

D

E

P

P

V

A

A

R

T

D

T

A

R

K

T
|
T

S

N

V

I

L

I

G

G

A

S

I

L

N

V

M

L

L

L

D

E

I

K

A

S

R

I

K

K

C

Y

K

G

A

V

K

K

-

K

-

F

I

I

L

F

Q
x
S

A
x
S

S
x
T

T

G

S
x
G

E
x
A

V

I

Y

Y

G

G

D

E

P

N

-

V

E

K

V

V

H

F

P

P

Q

T

P

P

E

E

S

T

-

E

Y

I

R

P

G

H

S

P

V

I

S

S

T

P

T
x
Y

G

G

I

I

R

-

A

-

C

-

Y

-

D

-

E

-

G

A

K
|
K

R

Y

C

S

A

T

E

E

T

M

L

Y

F

L

F

E

D

F

Y

F

Y

A

R

R

Q

E

H

Y

G

G

V

L

K

K

I

Y

K

T

V

V

I

L

R

R

I
x
Y

F

A

N
|
N

T
x
V

Y

Y

G

G

P

P

Y

R

M

Q

N

D

P

P

-
x
Y

D

G

D

E

G

A

R
x
G

V
|
V

V

V

S

A

N

I

F

F

I

T

M

E

Q

R

A

M

L

L

R

R

N

G

Q

E

D

E

I

V

T
x
H

I

I

Y
x
F

G

G

A

D

G

G

T

E

Q
x
Y

T

V

R
|
R

S

D

F

Y

Q

V

Y

Y

V

V

D

D

L

V

V

V

E

R

G

A

M

N

I

L

R

L

L

A

M

M

N

E

S

K

D

G

D

D

S

N

F

E

T

V

G

-

P

-

V

F

N

N

I

I

G

G

N

T

P

G

G

R

E

G

F

T

T

T

M
x
V

L

N

E

Q

L

L

A

F

Q

K

K

L

I

L

I

K

T

E

L

I

T

T

G

G

S

Y

S

D

S

K

K

E

L

P

T

V

Y

Y

K

K

P

P

L

P

P
x
R

Q

K

D

G

D
|
D

P

V

L

R

Q

K

R

S

Q

I

P

L

D

D

I

Y

S

T

L

K

A

A

K

K

K

E

E

K

L

L

D

G

W

W

E

E

P

P

T

K

I

V

A

S

L

L

E

E

G

G

L

L

K

K

K

L

T

T

I

V

A

E

Y

Y

F

F

K

R

active site: T117 (≠ S115), G119 (≠ S117), A120 (≠ E118), Y143 (≠ T139), K147 (= K149), Y181 (vs. gap), G185 (≠ R186)
binding nicotinamide-adenine-dinucleotide: G7 (= G9), G10 (= G12), F11 (= F13), I12 (≠ V14), D31 (= D33), N32 (= N34), S34 (≠ F36), S35 (≠ T37), G36 (= G38), S51 (≠ E53), I52 (≠ L54), L73 (= L73), A74 (= A74), A75 (≠ C75), T92 (= T92), S115 (≠ Q113), S116 (≠ A114), Y143 (≠ T139), K147 (= K149), Y170 (≠ I172), V173 (≠ T175)
binding uridine-5'-diphosphate-glucose: T117 (≠ S115), G119 (≠ S117), A120 (≠ E118), Y143 (≠ T139), N172 (= N174), G185 (≠ R186), V186 (= V187), H201 (≠ T202), F203 (≠ Y204), Y208 (≠ Q209), R210 (= R211), V244 (≠ M247), R267 (≠ P270), D270 (= D273)

6zllA Crystal structure of udp-glucuronic acid 4-epimerase from bacillus cereus in complex with udp-galacturonic acid and NAD (see paper)
30% identity, 99% coverage: 4:312/312 of query aligns to 2:315/321 of 6zllA

query
sites
6zllA

R

K

I

I

L

L

V

V

T

T

G
|
G

G

A

A

A

G
|
G

F
|
F

V
x
I

G

G

S

S

H

H

L

L

C

C

R

Q

R

A

L

L

-

K

N

N

E

S

G

A

N

Y

D

H

V

V

I

V

C

G

L

I

D
|
D

N
x
H

F
|
F

F
x
I

-

G

-

P

-

T

-

P

-

A

T

T

G

L

S
x
K

K

T

E

G

N

N

I

I

I

Q

D

S

L

L

L

E

P

L

H

N

P

S

Y

R

F

F

E

Q

L

F

I

I

R

R

H

E

D
|
D

V
x
I

Q

L

-

N

-

T

-

D

-

L

E

S

P

K

I

L

L

L

L

Q

E

D

V

I

D

D

E

V

I

V

Y

Y

N

H

L
|
L

A
|
A

C
x
A

-

I

P
|
P

A

G

S
x
V

P
x
R

P

T

H

S

Y

W

Q

G

F

K

D

D

P

F

V

Q

A

P

T

Y

T

V

R

T

T

N

S

N

V

I

L

M

G

V

A

T

I

Q

N

Q

M

L

L

L

D

E

I

A

A

C

R

K

K

H

C

I

K

K

A

L

-

D

K

K

I

F

L

I

Q

H

A
x
I

S

S

T
|
T

S
|
S

E
x
S

V

V

Y

Y

G

G

D

E

P

K

-

S

-

G

-

A

-

V

-

S

-

E

E

D

V

L

H

L

P

P

Q

I

P

P

E

L

S

S

Y

P

R
x
Y

G

G

S

V

V

T

S
x
K

T

L

T

S

G

G

I

E

R

H

A

L

C

C

Y

H

D

V

E

-

G

-

K

-

R

-

C

-

A

-

E

-

T

-

L

-

F

-

D

-

Y

Y

Y

H

R

K

Q

N

H

F

G

H

V

I

K

P

I

I

K

V

V

I

I

L

R

R

I
x
Y

F

F

N
x
T

T
x
V

Y

Y

G

G

P

P

Y

R

M

Q

N
x
R

P

P

D

D

D

-

G

-

R
x
M

V
x
A

V

F

S

H

N
x
R

F

L

I

I

M

K

Q

Q

A

M

L

L

R

E

N

D

Q

K

D

P

I

L

T
|
T

I

I

Y
x
F

G

G

A

D

G

G

T

T

Q
|
Q

T

T

R
|
R

S

D

F

F

Q

T

Y

Y

V

I

D

D

L

C

V

I

E

R

G

G

M

T

I

V

R

A

L

A

M

L

N

E

S

T

D

K

S

N

F

I

T

I

G

G

P

E

V

V

-

I

N

N

I

I

G

G

N

G

P

K

G

E

E

Q

F

A

T

S

M
x
I

L

L

E

D

L

I

A

I

Q

S

K

M

I

L

I

E

T

K

L

I

T

S

G

G

S

K

S

S

S

A

K

T

L

K

T

N

Y

F

K

L

P

K

L

S

P

V

Q

P

D

G

D
x
E

P

P

L

K

Q

Q

R

T

Q

W

P

A

D

D

I

I

S

S

L

K

A

A

K

S

K

T

E

L

L

L

D

Q

W

Y

E

S

P

P

T

T

I

V

A

S

L

L

E

S

E

D

G

G

L

L

K

E

K

A

T

E

I

Y

A

D

Y

Y

F

I

K

K

S

Q

L

L

T

Y

R

K

active site: T126 (= T116), S127 (= S117), S128 (≠ E118), Y149 (≠ R133), K153 (≠ S137)
binding nicotinamide-adenine-dinucleotide: G7 (= G9), G10 (= G12), F11 (= F13), I12 (≠ V14), D32 (= D33), H33 (≠ N34), F34 (= F35), I35 (≠ F36), K43 (≠ S39), D62 (= D58), I63 (≠ V59), L81 (= L73), A82 (= A74), A83 (≠ C75), I124 (≠ A114), T126 (= T116), Y149 (≠ R133), K153 (≠ S137), Y176 (≠ I172), V179 (≠ T175), R185 (≠ N181), M188 (≠ R186)
binding (2S,3R,4S,5R,6R)-6-[[[(2R,3S,4R,5R)-5-(2,4-dioxopyrimidin-1-yl)-3,4-dihydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl]oxy-hydroxy-phosphoryl]oxy-3,4,5-trihydroxy-oxane-2-carboxylic acid: P85 (= P76), V87 (≠ S78), R88 (≠ P79), T126 (= T116), S127 (= S117), Y149 (≠ R133), T178 (≠ N174), R185 (≠ N181), A189 (≠ V187), R192 (≠ N190), T204 (= T202), F206 (≠ Y204), Q211 (= Q209), R213 (= R211), I250 (≠ M247), E276 (≠ D273)

6zldA Crystal structure of udp-glucuronic acid 4-epimerase from bacillus cereus in complex with udp-glucuronic acid and NAD (see paper)
30% identity, 98% coverage: 4:310/312 of query aligns to 2:313/314 of 6zldA

query
sites
6zldA

R

K

I

I

L

L

V

V

T

T

G
|
G

G

A

A

A

G
|
G

F
|
F

V
x
I

G

G

S

S

H

H

L

L

C

C

R

Q

R

A

L

L

-

K

N

N

E

S

G

A

N

Y

D

H

V

V

I

V

C

G

L

I

D
|
D

N
x
H

F
|
F

F
x
I

-

G

-

P

-

T

-

P

-

A

T

T

G

L

S
x
K

K

T

E

G

N

N

I

I

I

Q

D

S

L

L

L

E

P

L

H

N

P

S

Y

R

F

F

E

Q

L

F

I

I

R

R

H

E

D
|
D

V
x
I

Q

L

-

N

-

T

-

D

-

L

E

S

P

K

I

L

L

L

L

Q

E

D

V

I

D

D

E

V

I

V

Y

Y

N

H

L
|
L

A
|
A

C
x
A

-

I

P
|
P

A

G

S

V

P
x
R

P

T

H

S

Y

W

Q

G

F

K

D

D

P

F

V

Q

A

P

T

Y

T

V

R

T

T

N

S

N

V

I

L

M

G

V

A

T

I

Q

N

Q

M

L

L

L

D

E

I

A

A

C

R

K

K

H

C

I

K

K

A

L

-

D

K

K

I

F

L

I

Q

H

A
x
I

S

S

T
|
T

S
|
S

E
x
S

V

V

Y

Y

G

G

D

E

P

K

-

S

-

G

-

A

-

V

-

S

-

E

E

D

V

L

H

L

P

P

Q

I

P

P

E

L

S

S

Y

P

R
x
Y

G

G

S

V

V

T

S
x
K

T

L

T

S

G

G

I

E

R

H

A

L

C

C

Y

H

D

V

E

-

G

-

K

-

R

-

C

-

A

-

E

-

T

-

L

-

F

-

D

-

Y

Y

Y

H

R

K

Q

N

H

F

G

H

V

I

K

P

I

I

K

V

V

I

I

L

R

R

I
x
Y

F
|
F

N
x
T

T

V

Y

Y

G

G

P

P

Y

R

M

Q

N
x
R

P

P

D

D

D

-

G

-

R
x
M

V
x
A

V

F

S

H

N
x
R

F

L

I

I

M

K

Q

Q

A

M

L

L

R

E

N

D

Q

K

D

P

I

L

T
|
T

I

I

Y
x
F

G

G

A

D

G

G

T

T

Q
|
Q

T

T

R
|
R

S

D

F

F

Q

T

Y

Y

V

I

D

D

L

C

V

I

E

R

G

G

M

T

I

V

R

A

L

A

M

L

N

E

S

T

D

K

S

N

F

I

T

I

G

G

P

E

V

V

-

I

N

N

I

I

G

G

N

G

P

K

G

E

E

Q

F

A

T

S

M
x
I

L

L

E

D

L

I

A

I

Q

S

K

M

I

L

I

E

T

K

L

I

T

S

G

G

S

K

S

S

S

A

K

T

L

K

T

N

Y

F

K

L

P

K

L

S

P

V

Q

P

D

G

D
x
E

P

P

L

K

Q

Q

R

T

Q

W

P

A

D

D

I

I

S

S

L

K

A

A

K

S

K

T

E

L

L

L

D

Q

W

Y

E

S

P

P

T

T

I

V

A

S

L

L

E

S

E

D

G

G

L

L

K

E

K

A

T

E

I

Y

A

D

Y

Y

F

I

K

K

S

Q

L

L

active site: T126 (= T116), S127 (= S117), S128 (≠ E118), Y149 (≠ R133), K153 (≠ S137)
binding nicotinamide-adenine-dinucleotide: G7 (= G9), G10 (= G12), F11 (= F13), I12 (≠ V14), D32 (= D33), H33 (≠ N34), F34 (= F35), I35 (≠ F36), K43 (≠ S39), D62 (= D58), I63 (≠ V59), L81 (= L73), A82 (= A74), A83 (≠ C75), I124 (≠ A114), T126 (= T116), K153 (≠ S137), Y176 (≠ I172), T178 (≠ N174), R185 (≠ N181), M188 (≠ R186)
binding uridine-5'-diphosphate-glucuronic acid: P85 (= P76), R88 (≠ P79), T126 (= T116), S127 (= S117), S128 (≠ E118), Y149 (≠ R133), F177 (= F173), T178 (≠ N174), R185 (≠ N181), M188 (≠ R186), A189 (≠ V187), R192 (≠ N190), T204 (= T202), F206 (≠ Y204), Q211 (= Q209), R213 (= R211), I250 (≠ M247), E276 (≠ D273)

6zl6A Crystal structure of udp-glucuronic acid 4-epimerase from bacillus cereus in complex with udp and NAD (see paper)
30% identity, 98% coverage: 4:310/312 of query aligns to 2:313/314 of 6zl6A

query
sites
6zl6A

R

K

I

I

L

L

V

V

T

T

G
|
G

G

A

A

A

G
|
G

F
|
F

V
x
I

G

G

S

S

H

H

L

L

C

C

R

Q

R

A

L

L

-

K

N

N

E

S

G

A

N

Y

D

H

V

V

I

V

C

G

L

I

D
|
D

N
x
H

F
|
F

F
x
I

-

G

-

P

-

T

-

P

-

A

T

T

G

L

S
x
K

K

T

E

G

N

N

I

I

I

Q

D

S

L

L

L

E

P

L

H

N

P

S

Y

R

F

F

E

Q

L

F

I

I

R

R

H

E

D
|
D

V
x
I

Q

L

-

N

-

T

-

D

-

L

E

S

P

K

I

L

L

L

L

Q

E

D

V

I

D

D

E

V

I

V

Y

Y

N

H

L
|
L

A
|
A

C
x
A

-

I

P

P

A

G

S

V

P

R

P

T

H

S

Y

W

Q

G

F

K

D

D

P

F

V

Q

A

P

T

Y

T

V

R

T

T

N

S

N

V

I

L

M

G

V

A

T

I

Q

N

Q

M

L

L

L

D

E

I

A

A

C

R

K

K

H

C

I

K

K

A

L

-

D

K

K

I

F

L

I

Q

H

A
x
I

S

S

T
|
T

S
|
S

E
x
S

V

V

Y

Y

G

G

D

E

P

K

-

S

-

G

-

A

-

V

-

S

-

E

E

D

V

L

H

L

P

P

Q

I

P

P

E

L

S

S

Y

P

R
x
Y

G

G

S

V

V

T

S
x
K

T

L

T

S

G

G

I

E

R

H

A

L

C

C

Y

H

D

V

E

-

G

-

K

-

R

-

C

-

A

-

E

-

T

-

L

-

F

-

D

-

Y

Y

Y

H

R

K

Q

N

H

F

G

H

V

I

K

P

I

I

K

V

V

I

I

L

R

R

I
x
Y

F

F

N
x
T

T
x
V

Y

Y

G

G

P

P

Y

R

M

Q

N
x
R

P

P

D

D

D

-

G

-

R
x
M

V
x
A

V

F

S

H

N
x
R

F

L

I

I

M

K

Q

Q

A

M

L

L

R

E

N

D

Q

K

D

P

I

L

T
|
T

I

I

Y
x
F

G

G

A

D

G

G

T

T

Q
|
Q

T

T

R
|
R

S

D

F

F

Q

T

Y

Y

V

I

D

D

L

C

V

I

E

R

G

G

M

T

I

V

R

A

L

A

M

L

N

E

S

T

D

K

S

N

F

I

T

I

G

G

P

E

V

V

-

I

N

N

I

I

G

G

N

G

P

K

G

E

E

Q

F

A

T

S

M
x
I

L

L

E

D

L

I

A

I

Q

S

K

M

I

L

I

E

T

K

L

I

T

S

G

G

S

K

S

S

S

A

K

T

L

K

T

N

Y

F

K

L

P

K

L

S

P

V

Q

P

D

G

D
x
E

P

P

L

K

Q

Q

R

T

Q

W

P

A

D

D

I

I

S

S

L

K

A

A

K

S

K

T

E

L

L

L

D

Q

W

Y

E

S

P

P

T

T

I

V

A

S

L

L

E

S

E

D

G

G

L

L

K

E

K

A

T

E

I

Y

A

D

Y

Y

F

I

K

K

S

Q

L

L

active site: T126 (= T116), S127 (= S117), S128 (≠ E118), Y149 (≠ R133), K153 (≠ S137)
binding nicotinamide-adenine-dinucleotide: G7 (= G9), G10 (= G12), F11 (= F13), I12 (≠ V14), D32 (= D33), H33 (≠ N34), F34 (= F35), I35 (≠ F36), K43 (≠ S39), D62 (= D58), I63 (≠ V59), L81 (= L73), A82 (= A74), A83 (≠ C75), I124 (≠ A114), T126 (= T116), K153 (≠ S137), Y176 (≠ I172), T178 (≠ N174), V179 (≠ T175), R185 (≠ N181), M188 (≠ R186)
binding uridine-5'-diphosphate: T178 (≠ N174), A189 (≠ V187), R192 (≠ N190), T204 (= T202), F206 (≠ Y204), Q211 (= Q209), R213 (= R211), I250 (≠ M247), E276 (≠ D273)

6zljA Crystal structure of udp-glucuronic acid 4-epimerase y149f mutant from bacillus cereus in complex with udp-4-deoxy-4-fluoro-glucuronic acid and NAD (see paper)
30% identity, 98% coverage: 4:310/312 of query aligns to 2:313/314 of 6zljA

query
sites
6zljA

R

K

I

I

L

L

V

V

T

T

G
|
G

G

A

A

A

G
|
G

F
|
F

V
x
I

G

G

S

S

H

H

L

L

C

C

R

Q

R

A

L

L

-

K

N

N

E

S

G

A

N

Y

D

H

V

V

I

V

C

G

L

I

D
|
D

N
x
H

F
|
F

F
x
I

-

G

-

P

-

T

-

P

-

A

T

T

G

L

S
x
K

K

T

E

G

N

N

I

I

I

Q

D

S

L

L

L

E

P

L

H

N

P

S

Y

R

F

F

E

Q

L

F

I

I

R

R

H

E

D
|
D

V
x
I

Q

L

-

N

-

T

-

D

-

L

E

S

P

K

I

L

L

L

L

Q

E

D

V

I

D

D

E

V

I

V

Y

Y

N

H

L
|
L

A
|
A

C
x
A

-

I

P
|
P

A

G

S

V

P
x
R

P

T

H

S

Y

W

Q

G

F

K

D

D

P

F

V

Q

A

P

T

Y

T

V

R

T

T

N

S

N

V

I

L

M

G

V

A

T

I

Q

N

Q

M

L

L

L

D

E

I

A

A

C

R

K

K

H

C

I

K

K

A

L

-

D

K

K

I

F

L

I

Q

H

A
x
I

S

S

T
|
T

S
|
S

E
x
S

V

V

Y

Y

G

G

D

E

P

K

-

S

-

G

-

A

-

V

-

S

-

E

E

D

V

L

H

L

P

P

Q

I

P

P

E

L

S

S

Y

P

R
x
F

G

G

S

V

V

T

S
x
K

T

L

T

S

G

G

I

E

R

H

A

L

C

C

Y

H

D

V

E

-

G

-

K

-

R

-

C

-

A

-

E

-

T

-

L

-

F

-

D

-

Y

Y

Y

H

R

K

Q

N

H

F

G

H

V

I

K

P

I

I

K

V

V

I

I

L

R

R

I
x
Y

F
|
F

N
x
T

T
x
V

Y

Y

G

G

P

P

Y

R

M

Q

N
x
R

P

P

D

D

D

-

G

-

R
x
M

V
x
A

V

F

S

H

N
x
R

F

L

I

I

M

K

Q

Q

A

M

L

L

R

E

N

D

Q

K

D

P

I

L

T
|
T

I

I

Y
x
F

G

G

A

D

G

G

T

T

Q
|
Q

T

T

R
|
R

S

D

F

F

Q

T

Y

Y

V

I

D

D

L

C

V

I

E

R

G

G

M

T

I

V

R

A

L

A

M

L

N

E

S

T

D

K

S

N

F

I

T

I

G

G

P

E

V

V

-

I

N

N

I

I

G

G

N

G

P

K

G

E

E

Q

F

A

T

S

M
x
I

L

L

E

D

L

I

A

I

Q

S

K

M

I

L

I

E

T

K

L

I

T

S

G

G

S

K

S

S

S

A

K

T

L

K

T

N

Y

F

K

L

P

K

L

S

P

V

Q

P

D

G

D
x
E

P

P

L

K

Q

Q

R

T

Q

W

P

A

D

D

I

I

S

S

L

K

A

A

K

S

K

T

E

L

L

L

D

Q

W

Y

E

S

P

P

T

T

I

V

A

S

L

L

E

S

E

D

G

G

L

L

K

E

K

A

T

E

I

Y

A

D

Y

Y

F

I

K

K

S

Q

L

L

active site: T126 (= T116), S127 (= S117), S128 (≠ E118), F149 (≠ R133), K153 (≠ S137)
binding nicotinamide-adenine-dinucleotide: G7 (= G9), G10 (= G12), F11 (= F13), I12 (≠ V14), D32 (= D33), H33 (≠ N34), F34 (= F35), I35 (≠ F36), K43 (≠ S39), D62 (= D58), I63 (≠ V59), L81 (= L73), A82 (= A74), A83 (≠ C75), I124 (≠ A114), T126 (= T116), K153 (≠ S137), Y176 (≠ I172), T178 (≠ N174), V179 (≠ T175), R185 (≠ N181), M188 (≠ R186)
binding (2~{R},3~{S},4~{R},5~{R},6~{R})-6-[[[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3-fluoranyl-4,5-bis(oxidanyl)oxane-2-carboxylic acid: P85 (= P76), R88 (≠ P79), T126 (= T116), S127 (= S117), S128 (≠ E118), F149 (≠ R133), F177 (= F173), T178 (≠ N174), R185 (≠ N181), M188 (≠ R186), A189 (≠ V187), R192 (≠ N190), T204 (= T202), F206 (≠ Y204), Q211 (= Q209), R213 (= R211), I250 (≠ M247), E276 (≠ D273)

8vr2B Crystal structure of the pcryo_0617 oxidoreductase/decarboxylase from psychrobacter cryohalolentis k5 in the presence of NAD and udp
32% identity, 98% coverage: 3:309/312 of query aligns to 8:313/321 of 8vr2B

query
sites
8vr2B

K

K

R

K

I

I

L

F

V

I

T

T

G
|
G

G

G

A

A

G
|
G

F
|
F

V
x
I

G

G

S

S

H

T

L

L

C

I

R

G

R

R

L

L

L

I

N

-

E

E

G

N

N

N

D

E

V

M

I

I

C

V

L

Y

D
|
D

N
|
N

F

L

F

-

T

-

G

-

S

-

K
x
E

E
x
R

N

N

I

T

I

L

D

K

L

S

L

Q

P

P

-

F

-

A

-

N

H

H

P

K

Y

N

F

L

E

T

L

L

I

I

R

Q

H

G

D
x
N

V
|
V

-

L

-

D

Q

Q

E

E

P

K

I

I

L

I

-

E

-

A

L

A

E

K

V

G

D

S

E

E

I

I

Y

F

-

I

N

H

L
x
A

A
|
A

C
x
A

P

I

A

A

S

G

P

I

P

D

H

N

Y

T

Q

V

F

K

D

S

P

P

V

V

A

R

T

T

T

M

R

T

T

V

S

N

V

M

L

I

G

G

A

T

I

A

N

N

M

A

L

L

D

E

I

A

A

A

R

H

K

Q

C

A

K

G

A

T

-

V

-

Q

K

R

I

F

L

L

Q

E

A
x
F

S

S

T

T

S

S

E

E

V

V

Y

F

G

G

D

-

P

-

E

-

V

-

H

-

P

-

Q

-

P

S

E

R

S

A

Y

Y

R

R

G

-

S

-

V

V

S

D

T

E

T

T

G

G

I

A

-

V

-

G

-

E

-

A

R
|
R

A

W

C

T

Y

Y

D

A

E

V

G

S

K
|
K

R

L

C

A

A

G

E

E

T

H

L

L

F

T

F

H

D

A

Y

Y

Y

N

R

R

Q

E

H

H

G

G

V

L

K

P

I

T

K

V

V

T

I

F

R

R

I
x
P

F

F

N
|
N

T

V

Y

Y

G

G

P

P

Y

G

M

Q

N

I

P

G

D

E

D
x
G

G
x
A

R

-

V

-

V

I

S

S

N

I

F
x
M

I

I

M

R

Q

K

A

A

L

L

R

N

N

N

Q

E

D

D

I

I

T
x
Y

I
|
I

Y
x
F

G

G

A

D

G

G

T

S

Q

Q

T

I

R
|
R

S

A

F

W

Q

C

Y

Y

V

V

D

D

L

M

V

I

E

D

G

A

M

L

I

M

R

K

L

A

M

L

N

S

S

V

D

P

D

Q

S

A

F

I

T

G

G

E

P

S

V

F

N

N

I

I

G

G

N

N

P

A

G

R

E

A

F

I

T

T

M

T

L
x
I

-

Y

E

G

L

L

A

A

Q

Q

K

T

I

I

I

C

T

R

L

V

T

L

G

N

S

S

S

K

S

S

K

E

L

I

T

I

Y

F

K

R

P

E

L

A

P

L

Q

S

D

A

D

D

P

I

L

E

Q

L

R

R

Q

I

P

P

D

N

I

V

S

D

L

K

A

S

K

E

E

L

L

L

D

G

W

F

E

K

P

A

T

Q

I

V

A

D

L

L

E

E

G

G

L

L

K

I

K

R

T

T

I

A

A

D

Y

W

F

L

K

S

S

A

binding nicotinamide-adenine-dinucleotide: G14 (= G9), G17 (= G12), F18 (= F13), I19 (≠ V14), D37 (= D33), N38 (= N34), E40 (≠ K40), R41 (≠ E41), N61 (≠ D58), V62 (= V59), A81 (≠ L73), A82 (= A74), A83 (≠ C75), F124 (≠ A114), K154 (= K149), P177 (≠ I172), N179 (= N174)
binding uridine-5'-diphosphate: R147 (= R142), G189 (≠ D184), A190 (≠ G185), M194 (≠ F191), Y205 (≠ T202), I206 (= I203), F207 (≠ Y204), R214 (= R211), I251 (≠ L248)

6bwlA X-ray structure of pal from bacillus thuringiensis (see paper)
31% identity, 98% coverage: 4:310/312 of query aligns to 2:312/313 of 6bwlA

query
sites
6bwlA

R

K

I

I

L

L

V

V

T

T

G
|
G

G

G

A

A

G
|
G

F
|
F

V
x
I

G

G

S

R

H

W

L

V

C

V

R

K

R

R

L

L

N

Q

E

D

G

K

N

H

D

E

V

V

I

W

C

I

L

L

D
|
D

N
|
N

F
x
L

F

A

T
x
N

G
x
S

S

T

K

T

E

A

N

N

I

I

I

T

D

E

L

F

L

A

P

H

H

D

P

L

Y

N

F

L

-

K

E

Q

L

C

I

I

R

Q

H

G

D
|
D

V
x
I

Q

K

E

D

P

K

I

K

L

L

L

V

-

A

-

Q

-

L

-

F

-

E

-

N

E

S

V

F

D

D

E

L

I

C

Y

Y

N

H

L
|
L

A
|
A

C
x
A

P

S

A
x
I

S

N

P

V

P

Q

H

D

Y

S

Q

I

F

D

D

D

P

A

V

R

A

A

T

T

T

F

R

E

T

N

S

D

V

T

L

I

G

G

A

T

I

F

N

N

M

L

L

L

D

E

I

Q

A

C

R

L

K

N

C

Y

K

D

A

V

K

K

I

M

L

V

Q

F

A
x
M

S

S

T
|
T

S
x
C

E
x
M

V

V

Y

Y

-

D

-

K

-

A

-

T

-

N

-

I

-

Q

G

G

D

I

P

S

E

E

V

L

H

D

P

P

-

I

Q

K

P

P

E

A

S

S

-

P

Y
|
Y

R

A

G

G

S

S

V

-

S

-

T

-

G

-

I

-

R

-

A

-

C

-

Y

-

D

-

E

-

G

-

K
|
K

R

I

C

A

A

A

E

E

T

N

L

M

F

V

F

L

D

S

Y

Y

R

Y

Q

A

H

Y

G

K