SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013461155.1 NCBI__GCF_000183725.1:WP_013461155.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q5F8J4 Homoserine dehydrogenase; HDH; HSD; NAD(+)-dependent homoserine dehydrogenase; NAD(+)-dependent HSD; NgHSD; EC 1.1.1.3 from Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) (see paper)
45% identity, 95% coverage: 20:418/419 of query aligns to 22:430/435 of Q5F8J4

query
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Q5F8J4

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L45 (= L45) mutation to R: Shows a marked increase in the catalytic efficiency with NADP(+).
LS 45:46 (= LS 45:46) mutation to RD: Does not impair the catalytic activity with NAD(+). Slightly increases the activity, but slightly decreases the affinity for NADP(+).; mutation to RR: Causes a shift in coenzyme preference from NAD(+) to NADP(+) by a factor of 974. Shows a slight decrease in the catalytic efficiency with NAD(+) and a 4.5-fold increase in catalytic efficiency with NADP(+).

6dzsA Mycobacterial homoserine dehydrogenase thra in complex with NADP
39% identity, 99% coverage: 4:418/419 of query aligns to 7:428/431 of 6dzsA

query
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binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G8), L12 (≠ V9), G13 (= G10), N14 (≠ T11), V15 (= V12), V45 (= V42), R46 (= R43), R47 (≠ D44), R52 (≠ S49), I63 (≠ Y60), L78 (= L74), M79 (= M75), P84 (≠ L80), A102 (= A98), K104 (= K100), G306 (= G295), T310 (= T299)

4pg7A Crystal structure of s. Aureus homoserine dehydrogenase at ph7.5 (see paper)
41% identity, 74% coverage: 3:310/419 of query aligns to 6:306/402 of 4pg7A

query
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4pg7A

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active site: D190 (= D194), K194 (= K198)
binding lysine: R215 (≠ E219), G219 (= G223)

Sites not aligning to the query:

binding lysine: 394, 395

6a0tB Homoserine dehydrogenase k99a mutant from thermus thermophilus hb8 complexed with hse and NADP+ (see paper)
35% identity, 78% coverage: 3:329/419 of query aligns to 5:325/332 of 6a0tB

query
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6a0tB

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E

H

A

R

W

Y

E

E

S

L

L

Q

R

E

P

I

F

A

A

G

E

D

E

I

G

P

L

F

I

E

Y

F

H

E

E

A

A

S

S

V

V

A

M

G

A

G

G

I

T

P

P

I

A

I

L

N

S

A

F

L

L

R

-

D

E

G

T

L

L

S

R

A

G

N

S

H

E

I

L

L

L

S

E

I

L

M

H

G

G

I

I

M

L

N
|
N

G
|
G

T
|
T

C

T

N

L

F

Y

M

I

L

L

T

Q

K

E

M

M

M

-

N

E

E

K

G

G

T

R

P

T

F

Y

A

A

E

E

V

A

L

L

A

L

E

E

A

A

Q

Q

A

R

L

L

G
|
G

Y
|
Y

A

A

E
|
E

A

A

D

D

P

P

T

T

F

L

D
|
D

I

V

G

E

G

G

F

I

D
|
D

A

A

H

H

K
|
K

L

L

L

T

I

L

L

L

A

A

S

R

I

L

A

L

Y

V

G

D

I

P

D

G

A

F

K

P

P

F

E

A

E

E

I

V

L

E

I

A

E

Q

G

G

I

I

E

A

G

R

I

L

T

T

P

P

A

E

D

V

I

L

A

Q

F

K

A

A

K

E

E

A

F

R

G

G

Y

E

T

R

I

V

K

R

L

L

L

V

G

A

I

S

A

L

K

F

R

G

D

E

D

G

A

G

E

R

V

W

E

R

L

A

R

A

V

V

H

A

A

P

A

R

L

R

V

L

K

P

E

Q

D

D

A

H

M

P

I

L

A

A

K

R

I

A

D

R

G

G

V

-

M

-

N

N

G

A

I

L

S

W

V

V

V

R

G

A

D

R

R

P

V

L

G

G

E

E

T

A

L

F

Y

V

Y

T

G

G

P

P

G

G

A
|
A

G
|
G

G

G

N

G

A

A

T
|
T

A

A

S

S

A

G

V

L

V

F

A

A

N

D

I

L

D

R

I

F

V

L

R

S

S

G

G

A

K

P

-

G

R

H

S

L

P

P

M

A

L

P

G

R

F

A

N

R

H

P

P

P

L

L

E

E

G

G

active site: D191 (= D194), K195 (= K198)
binding l-homoserine: N150 (= N152), G151 (= G153), T152 (= T154), Y178 (= Y181), E180 (= E183), D186 (= D189), K195 (= K198)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G8), G11 (≠ V9), G12 (= G10), T13 (= T11), V14 (= V12), L42 (≠ V41), V43 (= V42), R44 (= R43), D45 (= D44), K48 (= K47), R50 (≠ S49), A73 (≠ L74), M74 (= M75), G75 (= G76), A97 (= A98), N98 (= N99), G177 (= G180), E180 (= E183), A289 (= A294), G290 (= G295), T294 (= T299)

6a0sA Homoserine dehydrogenase from thermus thermophilus hb8 complexed with hse and NADPH (see paper)
34% identity, 68% coverage: 3:288/419 of query aligns to 5:283/331 of 6a0sA

query
sites
6a0sA

R

K

V

I

G

A

V

L

I

L

G

G

V
x
G

G
|
G

T
|
T

V
|
V

G

G

R

S

A

A

V

F

V

Y

Q

N

I

L

L

V

E

L

E

E

N

R

K

A

S

E

I

E

I

L

T

S

A

A

R

-

S

F

G

G

D

V

E

V

I

P

S

R

V

F

K

L

S

G

G

V

V
x
L

V
|
V

R
|
R

D
|
D

L

P

S

R

K
|
K

V

P

S
x
R

D

A

L

I

S

P

I

Q

A

E

V

L

S

L

Q

R

-

A

D

E

P

P

Y

F

D

D

I

L

V

L

D

-

P

-

E

E

I

A

D

D

I

L

V

V

E

E

L
x
A

M
|
M

G

G

V

V

E

E

L

A

P

P

L

L

A

R

V

L

V

V

K

L

K

P

A

A

L

L

Q

E

N

A

G

G

K

I

A

P

V

L

V

I

T

T

A
|
A

N

N

K
|
K

A

A

L

L

A

A

Y

E

H

A

R

W

Y

E

E

S

L

L

Q

R

E

P

I

F

A

A

G

E

D

E

I

G

P

L

F

I

E

Y

F

H

E

E

A

A

S

S

V

V

A

M

G

A

G

G

I

T

P

P

I

A

I

L

N

S

A

F

L

L

R

-

D

E

G

T

L

L

S

R

A

G

N

S

H

E

I

L

L

L

S

E

I

L

M

H

G

G

I

I

M

L

N
|
N

G
|
G

T
|
T

C

T

N

L

F

Y

M

I

L

L

T

Q

K

E

M

M

M

-

N

E

E

K

G

G

T

R

P

T

F

Y

A

A

E

E

V

A

L

L

A

L

E

E

A

A

Q

Q

A

R

L

L

G
|
G

Y
|
Y

A

A

E
|
E

A

A

D

D

P

P

T

T

F

L

D
|
D

I

V

G

E

G

G

F

I

D
|
D

A

A

H

H

K
|
K

L

L

L

T

I

L

L

L

A

A

S

R

I

L

A

L

Y

V

G

D

I

P

D

G

A

F

K

P

P

F

E

A

E

E

I

V

L

E

I

A

E

Q

G

G

I

I

E

A

G

R

I

L

T

T

P

P

A

E

D

V

I

L

A

Q

F

K

A

A

K

E

E

A

F

R

G

G

Y

E

T

R

I

V

K

R

L

L

L

V

G

A

I

S

A

L

K

F

R

G

D

E

D

G

A

G

E

R

V

W

E

R

L

A

R

A

V

V

H

A

A

P

A

R

L

R

V

L

K

P

E

Q

D

D

A

H

M

P

I

L

A

A

K

R

I

A

D

R

G

G

V

-

M

-

N

N

G

A

I

L

S

W

V

V

V

R

G

A

D

R

R

P

V

L

G

G

E

E

T

A

L

F

active site: D191 (= D194), K195 (= K198)
binding l-homoserine: K99 (= K100), N150 (= N152), G151 (= G153), T152 (= T154), Y178 (= Y181), E180 (= E183), D186 (= D189), K195 (= K198)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (≠ V9), G12 (= G10), T13 (= T11), V14 (= V12), L42 (≠ V41), V43 (= V42), R44 (= R43), D45 (= D44), K48 (= K47), R50 (≠ S49), A73 (≠ L74), M74 (= M75), A97 (= A98), K99 (= K100), G177 (= G180), E180 (= E183)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 289, 290, 294

2ejwA Homoserine dehydrogenase from thermus thermophilus hb8
34% identity, 68% coverage: 3:288/419 of query aligns to 5:283/331 of 2ejwA

query
sites
2ejwA

R

K

V

I

G

A

V

L

I

L

G

G

V

G

G

G

T

T

V

V

G

G

R

S

A

A

V

F

V

Y

Q

N

I

L

L

V

E

L

E

E

N

R

K

A

S

E

I

E

I

L

T

S

A

A

R

-

S

F

G

G

D

V

E

V

I

P

S

R

V

F

K

L

S

G

G

V

V

L

V

V

R

R

D

D

L

P

S

R

K

K

V

P

S

R

D

A

L

I

S

P

I

Q

A

E

V

L

S

L

Q

R

-

A

D

E

P

P

Y

F

D

D

I

L

V

L

D

-

P

-

E

E

I

A

D

D

I

L

V

V

E

E

L

A

M

M

G

G

V

V

E

E

L

A

P

P

L

L

A

R

V

L

V

V

K

L

K

P

A

A

L

L

Q

E

N

A

G

G

K

I

A

P

V

L

V

I

T

T

A

A

N

N

K

K

A

A

L

L

A

A

Y

E

H

A

R

W

Y

E

E

S

L

L

Q

R

E

P

I

F

A

A

G

E

D

E

I

G

P

L

F

I

E

Y

F

H

E
|
E

A

A

S

S

V
|
V

A
x
M

G
x
A

G

G

I
x
T

P

P

I

A

I

L

N

S

A

F

L

L

R

-

D

E

G

T

L

L

S

R

A

G

N

S

H

E

I

L

L

L

S

E

I

L

M

H

G

G

I

I

M

L

N

N

G

G

T

T

C

T

N

L

F

Y

M

I

L

L

T

Q

K

E

M

M

M

-

N

E

E

K

G

G

T

R

P

T

F

Y

A

A

E

E

V

A

L

L

A

L

E

E

A

A

Q

Q

A

R

L

L

G

G

Y

Y

A

A

E

E

A

A

D

D

P

P

T

T

F

L

D

D

I

V

G

E

G

G

F

I

D
|
D

A

A

H

H

K
|
K

L

L

L

T

I

L

L

L

A

A

S

R

I

L

A

L

Y

V

G

D

I

P

D

G

A

F

K

P

P

F

E

A

E

E

I

V

L

E

I

A

E

Q

G

G

I

I

E

A

G

R

I

L

T

T

P

P

A

E

D

V

I

L

A

Q

F

K

A

A

K

E

E

A

F

R

G

G

Y

E

T

R

I

V

K

R

L

L

L

V

G

A

I

S

A

L

K

F

R

G

D

E

D

G

A

G

E

R

V

W

E

R

L

A

R

A

V

V

H

A

A

P

A

R

L

R

V

L

K

P

E

Q

D

D

A

H

M

P

I

L

A

A

K

R

I

A

D

R

G

G

V

-

M

-

N

N

G

A

I

L

S

W

V

V

V

R

G

A

D

R

R

P

V

L

G

G

E

E

T

A

L

F

active site: D191 (= D194), K195 (= K198)
binding magnesium ion: E121 (= E122), V124 (= V125), M125 (≠ A126), A126 (≠ G127), T128 (≠ I129)

F9VNG5 Homoserine dehydrogenase; HDH; HSD; StHSD; EC 1.1.1.3 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 3 papers)
33% identity, 73% coverage: 6:312/419 of query aligns to 5:301/304 of F9VNG5

query
sites
F9VNG5

V

L

I

F

G

G

V
x
Y

G

G

T
x
N

V
|
V

G

G

R

K

A

A

V

F

V

R

Q

K

I

L

L

L

E

H

E

E

N

K

K

R

S

S

I

P

-

E

-

L

-

N

-

D

-

V

-

I

-

G

I

I

T

V

A

T

R
|
R

S
x
R

G

G

D

I

E

M

I

L

S

Q

V

D

K

K

S

E

G

D

V

F

V

T

R

P

D

D

L

L

S

E

K

-

V

-

S

G

D

D

L

V

S

F

I

K

A

A

V

F

S

E

Q

K

D

I

P

K

Y

P

D

D

I

I

V

I

D

-

P

-

E

-

I

V

D

D

I

V

V
x
S

V

S

E

A

L

N

M

Y

G

N

V

G

E

E

L

P

P

S

L

L

A

S

V

L

V

Y

K

K

K

E

A

A

L

I

Q

K

N

D

G

G

K

V

A

N

V

I

T

T

A
x
T

N

N

K
|
K

A

A

L

P

L

L

A

A

Y

L

H

A

R

F

Y

N

E

E

L

I

Q

F

E

S

I

L

A

A

G

R

D

S

-

K

-

G

I

V

P

K

F

I

E

G

F

F

E

Q

A

G

S

T

V

V

A

M

G

S

G

G

I

T

P

P

I

S

I

I

N

N

A

L

L

Y

R

R

D

-

G

V

L

L

S

P

A

G

N

S

H

R

I

V

L

I

S

K

I

I

M

R

G

G

I

I

M

L

N

N

G

G

T

T

C

T

N

N

F

F

M

I

L

L

T

T

K

-

M

L

M

M

N

N

E

K

G

G

T

V

P

S

F

F

A

E

E

E

V

A

L

L

A

K

E

E

A

A

Q

Q

A

R

L

R

G
|
G

Y

Y

A

A

E
|
E

A

E

D

D

P

P

T

T

F

L

D

D

I

I

G

N

G

G

F

F

D

D

A

A

H

A

K

K

L

I

L

T

I

I

L

L

A

A

S

N

I

F

A

M

Y

I

G

G

I

N

D

S

A

V

K

T

P

I

E

K

E

D

I

V

L

K

I

F

E

E

G

G

I

I

E

N

G

R

I

D

T

L

P

P

A

K

D

N

I

-

A

-

F

-

A

-

K

-

E

-

F

-

G

-

Y

E

T

K

I

I

K

K

L

L

L

I

G

A

I

Y

A

A

K

D

R

-

D

-

A

-

E

E

V

K

E

E

L

V

R

W

V

V

H

K

A

P

A

L

L

P

V

I

K

S

E

Q

D

D

A

D

M

P

I

L

A

Y

K

N

I

V

D

D

G

G

V

V

M

E

N

N

G

A

I

L

S

E

V

I

V

T

G

T

D

D

R

-

V

I

G

Q

E

S

T

I

L

L

Y

I

Y

R

G

G

P

P

G

G

A

A

G
|
G

G

P

N

V

A

N

T

A

A

A

S

Y

A

G

V

A

V

L

A

S

N

D

I

L

I

I

D

L

I

L

V

K

R

R

Y8 (≠ V9) binding NADP(+)
N10 (≠ T11) binding NADP(+)
V11 (= V12) binding NADP(+)
R38 (= R30) binding NADP(+)
R39 (≠ S31) binding NADP(+)
S73 (≠ V71) binding NADP(+)
T100 (≠ A98) binding NADP(+)
K102 (= K100) binding NADP(+)
G182 (= G180) binding NADP(+)
E185 (= E183) binding NADP(+)
G284 (= G295) binding NADP(+)

Sites not aligning to the query:

303 modified: Interchain

7f4cA The crystal structure of the immature holo-enzyme of homoserine dehydrogenase complexed with NADP and 1,4-butandiol from the hyperthermophilic archaeon sulfurisphaera tokodaii. (see paper)
33% identity, 73% coverage: 6:312/419 of query aligns to 5:297/300 of 7f4cA

query
sites
7f4cA

V

L

I
x
F

G
|
G

V
x
Y

G
|
G

T
x
N

V
|
V

G

G

R

K

A

A

V

F

V

R

Q

K

I

L

L

L

E

H

E

E

N

K

K

R

S

S

I

P

-

E

-

L

-

N

-

D

-

V

-

I

-

G

I

I

T

V

A
x
T

R
|
R

S
x
R

G

G

D

I

E

M

I

L

S

Q

V

D

K

K

S

E

G

D

V

F

V

T

R

P

D

D

L

L

S

E

K

-

V

-

S

G

D

D

L

V

S

F

I

K

A

A

V

F

S

E

Q

K

D

I

P

K

Y

P

D

D

I

I

V

I

D

-

P

-

E

-

I

V

D

D

I
x
V

V
x
S

E

A

L

N

M

Y

G

N

V

G

E

E

L

P

P

S

L

L

A

S

V

L

V

Y

K

K

K

E

A

A

L

I

Q

K

N

D

G

G

K

V

A

N

V

I

T

T

A
x
T

N

N

K
|
K

A

A

L

P

L

L

A

A

Y

L

H

A

R

F

Y

N

E

E

L

I

Q

F

E

S

I

L

A

A

G

R

D

S

-

K

-

G

I

V

P

K

F

I

E

G

F

F

E

Q

A
x
G

S

T

V

V

A

M

G
x
S

G

G

I

T

P

P

I

S

I

I

N

N

A

L

L

Y

R

R

D

-

G

V

L

L

S

P

A

G

N

S

H

R

I

V

L

I

S

K

I

I

M

R

G

G

I

I

M

L

N

N

G

G

T

T

C

T

N

N

F

F

M

I

L

L

T

T

K

-

M

L

M

M

N

N

E

K

G

G

T

V

P

S

F

F

A

E

E

E

V

A

L

L

A

K

E

E

A

A

Q

Q

A

R

L

R

G

G

Y

Y

A

A

E
|
E

A

E

D

D

P

P

T

T

F

L

D

D

I

I

G

N

G

G

F

F

D

D

A

A

H

A

K

K

L

I

L

T

I

I

L

L

A

A

S

N

I

F

A

M

Y

I

G

G

I

N

D

S

A

V

K

T

P

I

E

K

E

D

I

V

L

K

I

F

E

E

G

G

I

I

E

N

G

-

I

-

T

-

P

-

A

R

D

D

I

L

A

K

F

-

A

-

K

-

E

-

F

-

G

-

Y

-

T

-

I

I

K

K

L

L

L

I

G

A

I

Y

A

A

K

D

R

-

D

-

A

-

E

E

V

K

E

E

L

V

R

W

V

V

H

K

A

P

A

L

L

P

V

I

K

S

E

Q

D

D

A

D

M

P

I

L

A

Y

K

N

I

V

D

D

G

G

V

V

M

E

N

N

G

A

I

L

S

E

V

I

V

T

G

T

D

D

R

-

V

I

G

Q

E

S

T

I

L

L

Y

I

Y

R

G

G

P

P

G

G

A

A

G
|
G

G

P

N

V

A

N

T
x
A

A

A

S

Y

A

G

V

A

V

L

A

S

N

D

I

L

I

I

D

L

I

L

V

K

R

R

binding nadp nicotinamide-adenine-dinucleotide phosphate: F6 (≠ I7), G7 (= G8), Y8 (≠ V9), G9 (= G10), N10 (≠ T11), V11 (= V12), T37 (≠ A29), R38 (= R30), R39 (≠ S31), V72 (≠ I70), S73 (≠ V71), S74 (≠ V72), T100 (≠ A98), K102 (= K100), G127 (≠ A123), S131 (≠ G127), E185 (= E183), G280 (= G295), A284 (≠ T299)

5x9dA Crystal structure of homoserine dehydrogenase in complex with l- cysteine and NAD (see paper)
33% identity, 73% coverage: 6:312/419 of query aligns to 5:299/302 of 5x9dA

query
sites
5x9dA

V

L

I
x
F

G
|
G

V
x
Y

G
|
G

T
x
N

V
|
V

G

G

R

K

A

A

V

F

V

R

Q

K

I

L

L

L

E

H

E

E

N

K

K

R

S

S

I

P

-

E

-

L

-

N

-

D

-

V

-

I

-

G

I

I

T

V

A
x
T

R
|
R

S

R

G

G

D

I

E

M

I

L

S

Q

V

D

K

K

S

E

G

D

V

F

V

T

R

P

D

D

L

L

S

E

K

-

V

-

S

G

D

D

L

V

S

F

I

K

A

A

V

F

S

E

Q

K

D

I

P

K

Y

P

D

D

I

I

V

I

D

-

P

-

E

-

I

V

D

D

I
x
V

V
x
S

E

A

L

N

M

Y

G

N

V

G

E

E

L
x
P

P

S

L

L

A

S

V

L

V

Y

K

K

K

E

A

A

L

I

Q

K

N

D

G

G

K

V

A

N

V

I

T

T

A
x
T

N
|
N

K
|
K

A

A

L

P

L

L

A

A

Y

L

H

A

R

F

Y

N

E

E

L

I

Q

F

E

S

I

L

A

A

G

R

D

S

-

K

-

G

I

V

P

K

F

I

E

G

F

F

E

Q

A
x
G

S

T

V

V

A

M

G
x
S

G

G

I

T

P

P

I

S

I

I

N

N

A

L

L

Y

R

R

D

-

G

V

L

L

S

P

A

G

N

S

H

R

I

V

L

I

S

K

I

I

M

R

G

G

I

I

M

L

N
|
N

G
|
G

T
|
T

C

T

N

N

F

F

M

I

L

L

T

T

K

-

M

L

M

M

N

N

E

K

G

G

T

V

P

S

F

F

A

E

E

E

V

A

L

L

A

K

E

E

A

A

Q

Q

A

R

L

R

G

G

Y
|
Y

A
|
A

E
|
E

A

E

D

D

P

P

T

T

F

L

D
|
D

I

I

G

N

G

G

F

F

D
|
D

A

A

H

A

K
|
K

L

I

L

T

I

I

L

L

A

A

S

N

I

F

A

M

Y

I

G

G

I

N

D

S

A

V

K

T

P

I

E

K

E

D

I

V

L

K

I

F

E

E

G

G

I

I

E

N

G

R

I

-

T

D

P

P

A

K

D

N

I

-

A

-

F

-

A

-

K

-

E

-

F

-

G

-

Y

E

T

K

I

I

K

K

L

L

L

I

G

A

I

Y

A

A

K

D

R

-

D

-

A

-

E

E

V

K

E

E

L

V

R

W

V

V

H

K

A

P

A

L

L

P

V

I

K

S

E

Q

D

D

A

P

M

L

I

Y

A

-

K

N

I

V

D

D

G

G

V

V

M

E

N

N

G

A

I

L

S

E

V

I

V

T

G

T

D

D

R

-

V

I

G

Q

E

S

T

I

L

L

Y

I

Y

R

G

G

P

P

G

G

A
|
A

G
|
G

G

P

N

V

A

N

T
x
A

A

A

S

Y

A

G

V

A

V

L

A

S

N

D

I

L

I

I

D

L

I

L

V

K

R

R

active site: D196 (= D194), K200 (= K198)
binding (2R)-3-[[(4S)-3-aminocarbonyl-1-[(2R,3R,4S,5R)-5-[[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-3,4-bis(oxidanyl)oxolan-2-yl]-4H-pyridin-4-yl]sulfanyl]-2-azanyl-propanoic acid: F6 (≠ I7), G7 (= G8), Y8 (≠ V9), G9 (= G10), N10 (≠ T11), V11 (= V12), T37 (≠ A29), R38 (= R30), V72 (≠ I70), S73 (≠ V71), S74 (≠ V72), P82 (≠ L80), T100 (≠ A98), N101 (= N99), K102 (= K100), G127 (≠ A123), S131 (≠ G127), N155 (= N152), G156 (= G153), T157 (= T154), Y183 (= Y181), A184 (= A182), E185 (= E183), D191 (= D189), D196 (= D194), K200 (= K198), A281 (= A294), G282 (= G295), A286 (≠ T299)

4xb2A Hyperthermophilic archaeal homoserine dehydrogenase mutant in complex with NADPH (see paper)
31% identity, 73% coverage: 4:308/419 of query aligns to 5:309/319 of 4xb2A

query
sites
4xb2A

V

I

G

S

V

I

I
x
F

G

G

V
x
F

G
|
G

T
|
T

V
|
V

G

G

R

R

A

A

V

L

V

A

Q

E

I

I

L

I

E

A

E

E

N

K

K

S

S

R

I

I

-

F

-

G

-

V

-

E

-

L

-

N

-

V

-

I

-

S

I

I

T

T

A

D

R
|
R

S

S

G

G

-

T

-

I

-

W

-

G

-

D

-

F

-

D

-

L

-

E

-

A

D

K

E

E

I

V

S

A

V

E

K

S

S

T

G

G

V

-

R

-

D

K

L

L

S

S

K

N

V

I

S

G

D

D

L

Y

S

E

I

V

A

-

V

Y

S

N

Q

F

D

S

P

P

Y

Q

D

E

I

L

V

V

D

E

P

V

E

K

I

P

D

N

I

I

V

L

V

V

E

D

L
x
V

M
x
S

G
x
S

G

W

V

D

E

E

L

-

P

A

L

H

A

E

V

M

V

Y

K

K

K

V

A

A

L

L

Q

G

N

E

G

G

K

I

A

S

V

V

T

T

A
x
S

N
|
N

K
|
K

A

P

L

P

L

I

A

A

Y

N

H

Y

R

Y

Y

D

E

E

L

L

Q

M

E

N

I

L

A

A

-

K

-

E

G

N

D

N

I

A

P

G

F

I

E

F

F

F

E

E

A
x
S

S

T

V

V

A

M

G

A

G

G

I

T

P

P

I

I

N

G

A

V

L

L

R

R

D

E

G

N

L

L

S

L

A

G

N

E

H

N

I

I

L

K

S

R

I

I

M

D

G

A

I

V

M

V

N

N

G
x
A

T
x
S

C

T

N

T

F

F

M

I

L

L

T

T

K

K

M

M

M

-

N

S

E

E

G

G

T

K

P

T

F

L

A

D

E

D

V

A

L

I

A

E

E

E

A

A

Q

K

A

S

L

L

G

G

Y

I

A

L

E

E

A

E

D

D

P

P

T

S

F

K

D
|
D

I

I

G

D

G

G

F

I

D
|
D

A

A

A

Y

H

Y

K
|
K

L

A

L

K

I

I

L

L

A

H

S

W

I

V

A

S

Y

Y

G

G

I

E

D

P

A

P

K

E

P

E

E

E

I

R

L

L

I

-

E

-

G

G

I

I

E

R

G

E

I

V

T

R

P

D

A

A

D

-

I

-

A

-

F

-

A

-

K

-

E

-

F

-

G

-

Y

R

T

N

I

V

K

R

L

L

L

V

G

A

I

Q

A

V

K

S

R

K

D

G

D

K

A

I

E

S

V

V

E

K

L

P

R

R

V

-

H

-

A

-

A

K

L

L

V

S

K

S

E

D

D

N

A

P

M

L

I

L

A

-

K

-

I

V

D

E

G

G

V

V

M

Q

N

N

G

A

I

A

S

V

V

I

V

R

G

T

D

N

R

N

V

L

G

G

E

E

T

V

L

I

Y

L

Y

K

G

G

P

P

G

G

A
x
G

G

G

N

R

A

V

T
|
T

A

A

S

S

A

G

V

V

V

F

A

T

N

D

I

I

active site: D211 (= D194), K215 (= K198)
binding l-homoserine: A171 (≠ G153), S172 (≠ T154), D206 (= D189), K215 (= K198)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F8 (≠ I7), F10 (≠ V9), G11 (= G10), T12 (= T11), V13 (= V12), R40 (= R30), V91 (≠ L74), S92 (≠ M75), S93 (≠ G76), S114 (≠ A98), N115 (= N99), K116 (= K100), S141 (≠ A123), G295 (≠ A294), T300 (= T299)

4xb1A Hyperthermophilic archaeal homoserine dehydrogenase in complex with NADPH (see paper)
31% identity, 73% coverage: 4:308/419 of query aligns to 5:309/319 of 4xb1A

query
sites
4xb1A

V

I

G

S

V

I

I
x
F

G

G

V
x
F

G
|
G

T
|
T

V
|
V

G

G

R

R

A

A

V

L

V

A

Q

E

I

I

L

I

E

A

E

E

N

K

K

S

S

R

I

I

-

F

-

G

-

V

-

E

-

L

-

N

-

V

-

I

-

S

I

I

T

T

A
x
D

R
|
R

S

S

G

G

-

T

-

I

-

W

-

G

-

D

-

F

-

D

-

L

-

E

-

A

D

K

E

E

I

V

S
x
K

V

E

K

S

S

T

G

G

V

-

R

-

D

K

L

L

S

S

K

N

V

I

S

G

D

D

L

Y

S

E

I

V

A

-

V

Y

S

N

Q

F

D

S

P

P

Y

Q

D

E

I

L

V

V

D

E

P

V

E

K

I

P

D

N

I

I

V

L

V

V

E

D

L
x
V

M
x
S

G
x
S

G

W

V

D

E

E

L

-

P

A

L

H

A

E

V

M

V

Y

K

K

K

V

A

A

L

L

Q

G

N

E

G

G

K

I

A

S

V

V

T

T

A
x
S

N

N

K
|
K

A

P

L

P

L

I

A

A

Y

N

H

Y

R

Y

Y

D

E

E

L

L

Q

M

E

N

I

L

A

A

-

K

-

E

G

N

D

N

I

A

P

G

F

I

E

F

F

F

E

E

A
x
S

S

T

V

V

A

M

G

A

G

G

I

T

P

P

I

I

N

G

A

V

L

L

R

R

D

E

G

N

L

L

S

L

A

G

N

E

H

N

I

I

L

K

S

R

I

I

M

D

G

A

I

V

M

V

N

N

G

A

T

S

C

T

N

T

F

F

M

I

L

L

T

T

K

K

M

M

M

-

N

S

E

E

G

G

T

K

P

T

F

L

A

D

E

D

V

A

L

I

A

E

E

E

A

A

Q

K

A

S

L

L

G

G

Y

I

A

L

E

E

A

E

D

D

P

P

T

S

F

K

D

D

I

I

G

D

G

G

F

I

D
|
D

A

A

A

Y

H

Y

K
|
K

L

A

L

K

I

I

L

L

A

H

S

W

I

V

A

S

Y

Y

G

G

I

E

D

P

A

P

K

E

P

E

E

E

I

R

L

L

I

-

E

-

G

G

I

I

E

R

G

E

I

V

T

R

P

D

A

A

D

-

I

-

A

-

F

-

A

-

K

-

E

-

F

-

G

-

Y

R

T

N

I

V

K

R

L

L

L

V

G

A

I

Q

A

V

K

S

R

K

D

G

D

K

A

I

E

S

V

V

E

K

L

P

R

R

V

-

H

-

A

-

A

K

L

L

V

S

K

S

E

D

D

N

A

P

M

L

I

L

A

-

K

-

I

V

D

E

G

G

V

V

M

Q

N

N

G

A

I

A

S

V

V

I

V

R

G

T

D

N

R

N

V

L

G

G

E

E

T

V

L

I

Y

L

Y

K

G

G

P

P

G

G

A
x
G

G

G

N

R

A

V

T
|
T

A

A

S

S

A

G

V

V

V

F

A

T

N

D

I

I

active site: D211 (= D194), K215 (= K198)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F8 (≠ I7), F10 (≠ V9), G11 (= G10), T12 (= T11), V13 (= V12), D39 (≠ A29), R40 (= R30), K57 (≠ S36), V91 (≠ L74), S92 (≠ M75), S93 (≠ G76), S114 (≠ A98), K116 (= K100), S141 (≠ A123), G295 (≠ A294), T300 (= T299)

O58802 Homoserine dehydrogenase; HDH; HSD; HseDH; EC 1.1.1.3 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see paper)
31% identity, 73% coverage: 4:308/419 of query aligns to 5:309/319 of O58802

query
sites
O58802

V

I

G

S

V

I

I

F

G

G

V
x
F

G

G

T
|
T

V
|
V

G

G

R

R

A

A

V

L

V

A

Q

E

I

I

L

I

E

A

E

E

N

K

K

S

S

R

I

I

-

F

-

G

-

V

-

E

-

L

-

N

-

V

-

I

-

S

I

I

T

T

A

D

R
|
R

S

S

G

G

-

T

-

I

-

W

-

G

-

D

-

F

-

D

-

L

-

E

-

A

D

K

E

E

I

V

S
x
K

V

E

K

S

S

T

G

G

V

-

R

-

D

K

L

L

S

S

K

N

V

I

S

G

D

D

L

Y

S

E

I

V

A

-

V

Y

S

N

Q

F

D

S

P

P

Y

Q

D

E

I

L

V

V

D

E

P

V

E

K

I

P

D

N

I

I

V

L

V

V

E

D

L

V

M
x
S

G
x
S

G

W

V

D

E

E

L

-

P

A

L

H

A

E

V

M

V

Y

K

K

K

V

A

A

L

L

Q

G

N

E

G

G

K

I

A

S

V

V

T

T

A
x
S

N

N

K
|
K

A

P

L

P

L

I

A

A

Y

N

H

Y

R

Y

Y

D

E

E

L

L

Q

M

E

N

I

L

A

A

-

K

-

E

G

N

D

N

I

A

P

G

F

I

E

F

F

F

E
|
E

A

S

S

T

V
|
V

A

M

G
x
A

G

G

I
x
T

P

P

I

I

N

G

A

V

L

L

R

R

D

E

G

N

L

L

S

L

A

G

N

E

H

N

I

I

L

K

S

R

I

I

M

D

G

A

I

V

M

V

N

N

G

A

T

S

C

T

N

T

F

F

M

I

L

L

T

T

K

K

M

M

M

-

N

S

E

E

G

G

T

K

P

T

F

L

A

D

E

D

V

A

L

I

A

E

E

E

A

A

Q

K

A

S

L

L

G

G

Y

I

A

L

E

E

A

E

D

D

P

P

T

S

F

K

D

D

I

I

G

D

G

G

F

I

D

D

A

A

A

Y

H

Y

K

K

L

A

L

K

I

I

L

L

A

H

S

W

I

V

A

S

Y

Y

G

G

I

E

D

P

A

P

K

E

P

E

E

E

I

R

L

L

I

-

E

-

G

G

I

I

E

R

G

E

I

V

T

R

P

D

A

A

D

-

I

-

A

-

F

-

A

-

K

-

E

-

F

-

G

-

Y

R

T

N

I

V

K

R

L

L

L

V

G

A

I

Q

A

V

K

S

R

K

D

G

D

K

A

I

E

S

V

V

E

K

L

P

R

R

V

-

H

-

A

-

A

K

L

L

V

S

K

S

E

D

D

N

A

P

M

L

I

L

A

-

K

-

I

V

D

E

G

G

V

V

M

Q

N

N

G

A

I

A

S

V

V

I

V

R

G

T

D

N

R

N

V

L

G

G

E

E

T

V

L

I

Y

L

Y

K

G

G

P

P

G

G

A

G

G
|
G

G

G

N

R

A

V

T

T

A

A

S

S

A

G

V

V

V

F

A

T

N

D

I

I

F10 (≠ V9) binding NADPH
T12 (= T11) binding NADPH
V13 (= V12) binding NADPH
R40 (= R30) binding NADPH; mutation to A: Increases activity with NADP.
K57 (≠ S36) binding NADPH; mutation to A: Increases activity with NADP.
S92 (≠ M75) binding NADPH
S93 (≠ G76) binding NADPH
S114 (≠ A98) binding NADPH
K116 (= K100) binding NADPH
E140 (= E122) binding Na(+)
V143 (= V125) binding Na(+)
A145 (≠ G127) binding Na(+)
T147 (≠ I129) binding Na(+)
G296 (= G295) binding NADPH

3ingA Crystal structure of homoserine dehydrogenase (np_394635.1) from thermoplasma acidophilum at 1.95 a resolution
27% identity, 53% coverage: 3:222/419 of query aligns to 4:237/319 of 3ingA

query
sites
3ingA

R

R

V

I

G

I

V

L

I

M

G
|
G

V
x
T

G
|
G

T
x
N

V
|
V

G

G

R

L

A

N

V

V

V

L

Q

R

I

I

L

I

E

D

E

A

N

S

K

N

-

R

-

F

S

S

I

I

I

K

T

V

A

V

R

G

S

V

G

S

D
|
D

E
x
S

I

R

S

S

V

Y

K

A

S

S

G

G

V

R

V

N

R

L

D

D

L

I

S

S

-

S

-

I

-

S

-

N

-
x
K

-

E

-

K

-

T

-

G

K

R

V

I

S

S

D

D

L

R

S

A

I

F

A

S

V

G

S

P

Q

E

D

D

P

L

Y

M

D

G

I

E

V

A

D

A

D

D

P

L

E

L

I

V

D

D

I
x
C

V
x
T

V
x
P

E

A

L

S

M

R

G

D

G

G

V

V

E

R

L

-

P

E

L

Y

A

S

V

L

V

Y

K

R

K

M

A

A

L

F

Q

E

N

S

G

G

K

M

A

N

V

V

T

T

A
|
A

N
|
N

K
|
K

A

S

L

G

L

L

A

A

Y

N

H

K

R

W

Y

H

E

D

L

I

Q

M

E

D

I

S

A

A

G

N

D

Q

I

N

P

S

-

K

-

Y

F

I

E

R

F

Y

E

E

A
|
A

S

T

V

V

A

A

G

G

I

V

P

P

I

L

I

F

N

S

A

V

L

L

R

D

D

Y

G

S

L

I

S

L

A

P

N

S

H

K

I

V

L

K

S

R

I

F

M

R

G

G

I

I

M

V

N

S

G

S

T

T

C

I

N

N

F

Y

M

V

L

I

T

R

K

N

M

M

M

A

N

N

E

-

G

G

T

R

P

S

F

L

A

R

E

D

V

V

L

V

A

D

E

D

A

A

Q

I

A

K

L

K

G

G

Y

I

A

A

E
|
E

A
x
S

D

N

P

P

T

Q

F

D

D

D

I

L

G

N

G

G

F

L

D
|
D

A

A

H

R

K
|
K

L

S

L

V

I

I

L

L

A

V

S

N

I

H

A

I

Y

F

G

G

I

T

D

E

A

Y

K

T

P

L

E

N

E

D

I

V

L

E

I

Y

E

S

G

G

I

V

E

D

active site: D209 (= D194), K213 (= K198)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G9 (= G8), T10 (≠ V9), G11 (= G10), N12 (≠ T11), V13 (= V12), D38 (= D33), S39 (≠ E34), K57 (vs. gap), C85 (≠ I70), T86 (≠ V71), P87 (≠ V72), A112 (= A98), N113 (= N99), K114 (= K100), A139 (= A123), E198 (= E183), S199 (≠ A184)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 302, 305

3jsaA Homoserine dehydrogenase from thermoplasma volcanium complexed with NAD
35% identity, 33% coverage: 82:221/419 of query aligns to 101:239/321 of 3jsaA

query
sites
3jsaA

L

L

A

A

V

F

V

Y

K

K

K

E

A

T

L

F

Q

E

N

N

G

G

K

K

A

D

V

V

T

T

A
|
A

N
|
N

K

K

A

S

L

G

L

L

A

A

Y

N

H

F

R

W

Y

P

E

E

L

I

Q

M

E

E

I

Y

A

A

-

R

-

S

G

N

D

N

I

R

P

R

F

I

E

R

F

Y

E

E

A
|
A

S

T

V

V

A

A

G

G

I

V

P

P

I

L

I

F

N

S

A

F

L

I

R

D

D

Y

G

S

L

V

S

L

A

P

N

S

H

R

I

I

L

K

S

K

I

F

M

R

G

G

I

I

M

V

N

S

G

L

T

T

C

I

N

N

F

Y

M

F

L

I

T

R

K

E

M

L

M

A

N

N

E

K

G

-

T

R

P

E

F

F

A

D

E

D

V

V

L

L

A

S

E

E

A

A

Q

T

A

K

L

L

G

G

Y

I

A

V

E

N

A

Y

D

K

P

D

T

-

F

-

D

D

I

L

G

T

G

G

F

L

D
|
D

A

A

H

R

K
|
K

L

S

L

V

I

I

L

L

A

C

S

N

I

H

A

L

Y

Y

G

G

I

S

D

S

A

Y

K

R

P

L

E

S

E

D

I

V

L

F

I

Y

E

E

G

G

I

I

active site: D212 (= D194), K216 (= K198)
binding nicotinamide-adenine-dinucleotide: A117 (= A98), N118 (= N99), A144 (= A123)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 10, 11, 12, 13, 14, 42, 43, 90, 91, 92, 308

O81852 Bifunctional aspartokinase/homoserine dehydrogenase 2, chloroplastic; AK-HD 2; AK-HSDH 2; Beta-aspartyl phosphate homoserine 2; EC 2.7.2.4; EC 1.1.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
28% identity, 53% coverage: 89:310/419 of query aligns to 662:908/916 of O81852

query
sites
O81852

L

L

Q

R

N

K

G

G

K

I

A

H

V

V

V

I

T

T

A

P

N

N

K

K

-

K

-

A

-

N

-

S

-

G

A

P

L

L

L

D

A

Q

Y

Y

H

L

R

K

Y

L

E

R

L

D

Q

L

E

Q

I

R

A

K

G

S

D

Y

I

T

P

H

F

Y

E

F

F

Y

E

E

A

A

S

T

V

V

A

G

G

A

G

G

I

L

P

P

I

I

N

S

A

T

L

L

R

R

D

G

G

L

L

L

-

E

S

T

A

G

N

D

H

K

I

I

L

L

S

R

I

I

M

E

G

G

I

I

M

C

N

S

G

G

T

T

C

L

N

S

F

Y

M

L

L

F

T

N

K

N

M

F

M

V

N

G

E

D

G

R

T

S

P

-

F

F

A

S

E

E

V

V

L

V

A

T

E

E

A

A

Q

K

A

N

L

A

G

G

Y

F

A

T

E

E

A

P

D

D

P

P

T

R

F

D

D

D

I

L

G

S

G

G

F

T

D

D

A

V

A

A

H

R

K

K

L

V

L

I

I

I

L

L

A

A

S

R

I

E

A

S

Y

-

G

G

I

L

-

K

-

L

-

D

-

L

-

A

D

D

A

L

K

P

P

I

E

R

E

S

I

L

L

V

I

P

E

E

G

P

I

L

E

K

G

G

I

C

T

T

P

S

A

V

-

E

-

F

-

M

-

E

-

K

-

L

-

P

-

Q

-

Y

D

D

I

G

A

D

F

L

A

A

K

K

E

E

-

R

-

L

-

D

-

A

-

E

-

N

F

S

G

G

Y

E

T

V

I

L

K

R

L

Y

L

V

G

G

I

V

-

V

-

D

A

A

K

V

R

N

D

Q

D

K

A

G

E

T

V

V

E

E

L

L

R

R

V

R

H

Y

A

-

L

-

V

-

K

K

E

K

D

E

A

H

M

P

I

F

A

A

K

Q

I

L

D

A

G

G

V

S

M

D

N

N

G

I

I

I

S

A

V

F

V

T

G

T

D

T

R

R

V

Y

G

K

E

D

-

H

-

P

T

L

L

I

Y

V

Y

R

G

G

P

P

G

G

A

A

G

G

G

A

N

Q

A

V

T

T

A

A

S

G

A

G

V

I

V

F

A

S

N

D

I

I

I

L

D

R

I

L

Sites not aligning to the query:

441 I→A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
443 Q→A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
522 I→A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.
524 Q→A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.

P31116 Homoserine dehydrogenase; HDH; HSD; EC 1.1.1.3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
27% identity, 67% coverage: 1:279/419 of query aligns to 5:300/359 of P31116

query
sites
P31116

M

V

T

V

R

N

V

V

G

A

V

V

I

I

G

G

V
x
A

G

G

T
x
V

V
|
V

G

G

R

S

A

A

V

F

V

L

Q

D

I

Q

L

L

E

L

E

A

N

M

K

K

S

S

I

T

I

I

T

T

A

Y

R

N

-

L

-

V

-

L

S

A

G

E

D
x
A

E

E

I

R

S

S

V

L

K

I

S

S

G

K

V

D

V

F

R

S

D

P

L

L

S

N

K

V

V

G

S

S

D

D

L

W

S

K

I

A

A

A

V

L

S

A

Q

A

D

S

P

T

Y

T

D

K

I

T

V

L

D

-

P

P

E

L

I

D

D

D

I

L

V

I

V

A

E
x
H

L

L

M

K

G

T

G

S

V

P

E

K

L

-

P

P

L

V

A

I

V

L

V

V

-

D

-

N

-
x
T

-

S

-

A

-

Y

-

I

-

A

-

G

-

F

-

Y

K

T

K

K

A

F

L

V

Q

E

N

N

G

G

K

I

A

S

V

I

V

A

T

T

A

P

N

N

K

K

A

K

L

A

L

F

A

S

Y

S

H

D

R

L

Y

A

E

T

L

W

Q

K

E

A

I

L

A

F

G

S

D

N

I

K

P

P

-

T

-

N

-

G

F

F

E

V

F

Y

-

H

E
|
E

A

A

S

T

V
|
V

A

G

G
x
A

G

G

I
x
L

P

P

I

I

N

S

A

F

L

L

R

R

D

E

G

I

L

I

-

Q

S

T

A

G

N

D

H

E

I

V

L

E

S

K

I

I

M

E

G

G

I

I

M

F

N

S

G

G

T

T

C

L

N

S

F

Y

M

I

L

F

-

N

-

E

-

F

-

S

T

T

K

S

M

Q

M

A

N

N

E

D

G

-

T

V

P

K

F

F

A

S

E

D

V

V

L

V

A

K

E

V

A

A

Q

K

A

K

L

L

G

G

Y

Y

A

T

E
|
E

A

P

D

D

P

P

T

R

F

D

D

D

I

L

G

N

G

G

F

L

D
|
D

A

V

A

A

H

R

K
|
K

L

V

L

T

I

I

L

V

A

G

S

R

I

I

A

S

Y

-

G

G

I

V

D

E

A

V

K

E

-

S

-

P

-

T

-

S

-

F

P

P

E

V

E

Q

I

S

L

L

I

I

-

P

E

K

G

P

I

L

E

E

G

S

I

V

T

K

P

S

A

A

D

D

I

E

A

F

F

L

A

E

K

K

E

L

F

S

G

D

Y

Y

T

D

I

K

K

D

L

L

L

T

G

Q

I

L

A

K

K

K

R

-

D

-

D

E

A

A

E

A

V

T

E

E

L

N

R

K

V

V

H

L

A

-

L

-

V

-

K

-

E

-

D

-

A

R

M

F

I

I

A

G

K

K

I

V

D

D

G

V

V

A

M

T

N

K

G

S

I

V

S

S

V

V

A13 (≠ V9) binding NAD(+)
V15 (≠ T11) binding NAD(+)
V16 (= V12) binding NAD(+)
A41 (≠ D33) binding NAD(+)
H79 (≠ E73) mutation to A: Reduces kcat 2-fold.
T93 (vs. gap) binding NAD(+)
E143 (= E122) binding Na(+)
V146 (= V125) binding Na(+)
A148 (≠ G127) binding Na(+)
L150 (≠ I129) binding Na(+)
E208 (= E183) binding L-homoserine; mutation to D: Increases KM for aspartate-semialdehyde 48-fold and reduces kcat by 50%.; mutation E->L,Q: Loss of activity.
D219 (= D194) binding L-homoserine; mutation to L: Reduces kcat 150-fold.
K223 (= K198) mutation to V: Loss of activity.

Sites not aligning to the query:

309 H→A: Reduces kcat 40-fold. Affects dimer formation.
340 binding NAD(+)

1tveA Homoserine dehydrogenase in complex with 4-(4-hydroxy-3- isopropylphenylthio)-2-isopropylphenol (see paper)
27% identity, 67% coverage: 1:279/419 of query aligns to 4:299/358 of 1tveA

query
sites
1tveA

M

V

T

V

R

N

V

V

G

A

V

V

I

I

G

G

V

A

G

G

T

V

V

V

G

G

R

S

A

A

V

F

V

L

Q

D

I

Q

L

L

E

L

E

A

N

M

K

K

S

S

I

T

I

I

T

T

A

Y

R

N

-

L

-

V

-

L

S

A

G

E

D

A

E

E

I

R

S

S

V

L

K

I

S

S

G

K

V

D

V

F

R

S

D

P

L

L

S

N

K

V

V

G

S

S

D

D

L

W

S

K

I

A

A

A

V

L

S

A

Q

A

D

S

P

T

Y

T

D

K

I

T

V

L

D

-

P

P

E

L

I

D

D

D

I

L

V

I

V

A

E

H

L

L

M

K

G

T

G

S

V

P

E

K

L

-

P

P

L

V

A

I

V

L

V

V

-

D

-

N

-

T

-
x
S

-

S

-

A

-

Y

-

I

-

A

-

G

-

F

-

Y

K

T

K

K

A

F

L

V

Q

E

N

N

G

G

K

I

A

S

V

I

V

A

T

T

A

P

N

N

K
|
K

A

K

L

A

L

F

A

S

Y

S

H

D

R

L

Y

A

E

T

L

W

Q

K

E

A

I

L

A

F

G

S

D

N

I

K

P

P

-

T

-

N

-

G

F

F

E

V

F

Y

-

H

E

E

A

A

S

T

V

V

A

G

G

A

G

G

I

L

P

P

I

I

N

S

A

F

L

L

R

R

D

E

G

I

L

I

-

Q

S

T

A

G

N

D

H

E

I

V

L

E

S

K

I

I

M

E

G

G

I

I

M

F

N

S

G

G

T
|
T

C

L

N

S

F

Y

M

I

L

F

-

N

-

E

-

F

-

S

T

T

K

S

M

Q

M

A

N

N

E

D

G

-

T

V

P

K

F

F

A

S

E

D

V

V

L

V

A

K

E

V

A

A

Q

K

A

K

L

L

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

T

R

F

D

D
|
D

I

L

G

N

G

G

F

L

D
|
D

A

V

A

A

H

R

K
|
K

L

V

L

T

I

I

L

V

A

G

S

R

I

I

A

S

Y

-

G

G

I

V

D

E

A

V

K

E

-

S

-

P

-

T

-

S

-

F

P

P

E

V

E

Q

I

S

L

L

I

I

-

P

E

K

G

P

I

L

E

E

G

S

I

V

T

K

P

S

A

A

D

D

I

E

A

F

F

L

A

E

K

K

E

L

F

S

G

D

Y

Y

T

D

I

K

K

D

L

L

L

T

G

Q

I

L

A

K

K

K

R

-

D

-

D

E

A

A

E

A

V

T

E

E

L

N

R

K

V

V

H

L

A

-

L

-

V

-

K

-

E

-

D

-

A

R

M

F

I

I

A

G

K

K

I

V

D

D

G

V

V

A

M

T

N

K

G

S

I

V

S

S

V

V

active site: D218 (= D194), K222 (= K198)
binding 4-(4-hydroxy-3-isopropylphenylthio)-2-isopropylphenol: S93 (vs. gap), K116 (= K100), T175 (= T154), D213 (= D189), D218 (= D194)

1q7gA Homoserine dehydrogenase in complex with suicide inhibitor complex NAD-5-hydroxy-4-oxonorvaline (see paper)
27% identity, 67% coverage: 1:279/419 of query aligns to 4:299/358 of 1q7gA

query
sites
1q7gA

M

V

T

V

R

N

V

V

G

A

V

V

I

I

G

G

V

A

G
|
G

T
x
V

V
|
V

G

G

R

S

A

A

V

F

V

L

Q

D

I

Q

L

L

E

L

E

A

N

M

K

K

S

S

I

T

I

I

T

T

A

Y

R

N

-

L

-

V

-

L

S

A

G
x
E

D

A

E

E

I

R

S

S

V

L

K

I

S

S

G

K

V

D

V

F

R

S

D

P

L

L

S

N

K

V

V

G

S

S

D

D

L

W

S

K

I

A

A

A

V

L

S

A

Q

A

D

S

P

T

Y

T

D

K

I

T

V

L

D

-

P

P

E

L

I

D

D

D

I

L

V

I

V

A

E

H

L

L

M

K

G

T

G

S

V

P

E

K

L

-

P

P

L

V

A

I

V

L

V

V

-

D

-
x
N

-
x
T

-
x
S

-

S

-

A

-

Y

-
x
I

-

A

-

G

-

F

-

Y

K

T

K

K

A

F

L

V

Q

E

N

N

G

G

K

I

A

S

V

I

V

A

T

T

A
x
P

N

N

K
|
K

A

K

L

A

L

F

A

S

Y

S

H

D

R

L

Y

A

E

T

L

W

Q

K

E

A

I

L

A

F

G

S

D

N

I

K

P

P

-

T

-

N

-

G

F

F

E

V

F

Y

-

H

E

E

A
|
A

S

T

V

V

A

G

G

A

G

G

I

L

P

P

I

I

N

S

A

F

L

L

R

R

D

E

G

I

L

I

-

Q

S

T

A

G

N

D

H

E

I

V

L

E

S

K

I

I

M

E

G

G

I

I

M

F

N
x
S

G

G

T

T

C

L

N

S

F

Y

M

I

L

F

-

N

-

E

-

F

-

S

T

T

K

S

M

Q

M

A

N

N

E

D

G

-

T

V

P

K

F

F

A

S

E

D

V

V

L

V

A

K

E

V

A

A

Q

K

A

K

L

L

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

T

R

F

D

D

D

I

L

G

N

G

G

F

L

D
|
D

A

V

A

A

H

R

K
|
K

L

V

L

T

I

I

L

V

A

G

S

R

I

I

A

S

Y

-

G

G

I

V

D

E

A

V

K

E

-

S

-

P

-

T

-

S

-

F

P

P

E

V

E

Q

I

S

L

L

I

I

-

P

E

K

G

P

I

L

E

E

G

S

I

V

T

K

P

S

A

A

D

D

I

E

A

F

F

L

A

E

K

K

E

L

F

S

G

D

Y

Y

T

D

I

K

K

D

L

L

L

T

G

Q

I

L

A

K

K

K

R

-

D

-

D

E

A

A

E

A

V

T

E

E

L

N

R

K

V

V

H

L

A

-

L

-

V

-

K

-

E

-

D

-

A

R

M

F

I

I

A

G

K

K

I

V

D

D

G

V

V

A

M

T

N

K

G

S

I

V

S

S

V

V

active site: D218 (= D194), K222 (= K198)
binding nicotinamide-adenine-dinucleotide-5-hydroxy-4-oxonorvaline: G13 (= G10), V14 (≠ T11), V15 (= V12), E39 (≠ G32), N91 (vs. gap), T92 (vs. gap), S93 (vs. gap), I97 (vs. gap), P114 (≠ A98), K116 (= K100), A143 (= A123), S173 (≠ N152), K222 (= K198)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide-5-hydroxy-4-oxonorvaline: 338, 343

1ebuD Homoserine dehydrogenase complex with NAD analogue and l-homoserine (see paper)
27% identity, 67% coverage: 1:279/419 of query aligns to 4:299/358 of 1ebuD

query
sites
1ebuD

M

V

T

V

R

N

V

V

G

A

V

V

I

I

G
|
G

V
x
A

G
|
G

T
x
V

V
|
V

G

G

R

S

A

A

V

F

V

L

Q

D

I

Q

L

L

E

L

E

A

N

M

K

K

S

S

I

T

I

I

T

T

A

Y

R

N

-

L

-

V

-

L

S

A

G
x
E

D
x
A

E

E

I

R

S

S

V

L

K

I

S

S

G

K

V

D

V

F

R

S

D

P

L

L

S

N

K

V

V

G

S

S

D

D

L

W

S

K

I

A

A

A

V

L

S

A

Q

A

D

S

P

T

Y

T

D

K

I

T

V

L

D

-

P

P

E

L

I

D

D

D

I

L

V

I

V

A

E

H

L

L

M

K

G

T

G

S

V

P

E

K

L

-

P

P

L

V

A

I

V

L

V

V

-

D

-
x
N

-

T

-
x
S

-

S

-

A

-

Y

-

I

-

A

-

G

-

F

-

Y

K

T

K

K

A

F

L

V

Q

E

N

N

G

G

K

I

A

S

V

I

V

A

T

T

A

P

N

N

K
|
K

A

K

L

A

L

F

A

S

Y

S

H

D

R

L

Y

A

E

T

L

W

Q

K

E

A

I

L

A

F

G

S

D

N

I

K

P

P

-

T

-

N

-

G

F

F

E

V

F

Y

-

H

E

E

A

A

S

T

V

V

A

G

G

A

G

G

I

L

P

P

I

I

N

S

A

F

L

L

R

R

D

E

G

I

L

I

-

Q

S

T

A

G

N

D

H

E

I

V

L

E

S

K

I

I

M

E

G

G

I

I

M

F

N

S

G

G

T

T

C

L

N

S

F

Y

M

I

L

F

-

N

-

E

-

F

-

S

T

T

K

S

M

Q

M

A

N

N

E

D

G

-

T

V

P

K

F

F

A

S

E

D

V

V

L

V

A

K

E

V

A

A

Q

K

A

K

L

L

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

T

R

F

D

D

D

I

L

G

N

G

G

F

L

D
|
D

A

V

A

A

H

R

K
|
K

L

V

L

T

I

I

L

V

A

G

S

R

I

I

A

S

Y

-

G

G

I

V

D

E

A

V

K

E

-

S

-

P

-

T

-

S

-

F

P

P

E

V

E

Q

I

S

L

L

I

I

-

P

E

K

G

P

I

L

E

E

G

S

I

V

T

K

P

S

A

A

D

D

I

E

A

F

F

L

A

E

K

K

E

L

F

S

G

D

Y

Y

T

D

I

K

K

D

L

L

L

T

G

Q

I

L

A

K

K

K

R

-

D

-

D

E

A

A

E

A

V

T

E

E

L

N

R

K

V

V

H

L

A

-

L

-

V

-

K

-

E

-

D

-

A

R

M

F

I

I

A

G

K

K

I

V

D

D

G

V

V

A

M

T

N

K

G

S

I

V

S

S

V

V

active site: D218 (= D194), K222 (= K198)
binding 3-aminomethyl-pyridinium-adenine-dinucleotide: G11 (= G8), A12 (≠ V9), G13 (= G10), V14 (≠ T11), V15 (= V12), E39 (≠ G32), A40 (≠ D33), N91 (vs. gap), S93 (vs. gap), K116 (= K100)

Sites not aligning to the query:

binding 3-aminomethyl-pyridinium-adenine-dinucleotide: 343

1ebfA Homoserine dehydrogenase from s. Cerevisiae complex with NAD+ (see paper)
27% identity, 67% coverage: 1:279/419 of query aligns to 4:299/358 of 1ebfA

query
sites
1ebfA

M

V

T

V

R

N

V

V

G

A

V

V

I
|
I

G

G

V
x
A

G
|
G

T
x
V

V
|
V

G

G

R

S

A

A

V

F

V

L

Q

D

I

Q

L

L

E

L

E

A

N

M

K

K

S

S

I

T

I

I

T

T

A

Y

R

N

-

L

-

V

-

L

S

A

G
x
E

D
x
A

E

E

I

R

S

S

V

L

K

I

S

S

G

K

V

D

V

F

R

S

D

P

L

L

S

N

K

V

V

G

S

S

D

D

L

W

S

K

I

A

A

A

V

L

S

A

Q

A

D

S

P

T

Y

T

D

K

I

T

V

L

D

-

P

P

E

L

I

D

D

D

I

L

V

I

V

A

E

H

L

L

M

K

G

T

G

S

V

P

E

K

L

-

P

P

L

V

A

I

V

L

V

V

-

D

-

N

-
x
T

-
x
S

-

S

-

A

-

Y

-

I

-

A

-

G

-

F

-

Y

K

T

K

K

A

F

L

V

Q

E

N

N

G

G

K

I

A

S

V

I

V

A

T

T

A
x
P

N

N

K

K

A

K

L

A

L

F

A

S

Y

S

H

D

R

L

Y

A

E

T

L

W

Q

K

E

A

I

L

A

F

G

S

D

N

I

K

P

P

-

T

-

N

-

G

F

F

E

V

F

Y

-

H

E

E

A

A

S

T

V

V

A

G

G

A

G

G

I

L

P

P

I

I

N

S

A

F

L

L

R

R

D

E

G

I

L

I

-

Q

S

T

A

G

N

D

H

E

I

V

L

E

S

K

I

I

M

E

G

G

I

I

M

F

N

S

G

G

T

T

C

L

N

S

F

Y

M

I

L

F

-

N

-

E

-

F

-

S

T

T

K

S

M

Q

M

A

N

N

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D

G

-

T

V

P

K

F

F

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D

V

V

L

V

A

K

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V

A

A

Q

K

A

K

L

L

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

T

R

F

D

D

D

I

L

G

N

G

G

F

L

D
|
D

A

V

A

A

H

R

K
|
K

L

V

L

T

I

I

L

V

A

G

S

R

I

I

A

S

Y

-

G

G

I

V

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A

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K

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-

S

-

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-

T

-

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-

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P

P

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E

Q

I

S

L

L

I

I

-

P

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K

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P

I

L

E

E

G

S

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V

T

K

P

S

A

A

D

D

I

E

A

F

F

L

A

E

K

K

E

L

F

S

G

D

Y

Y

T

D

I

K

K

D

L

L

L

T

G

Q

I

L

A

K

K

K

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-

D

-

D

E

A

A

E

A

V

T

E

E

L

N

R

K

V

V

H

L

A

-

L

-

V

-

K

-

E

-

D

-

A

R

M

F

I

I

A

G

K

K

I

V

D

D

G

V

V

A

M

T

N

K

G

S

I

V

S

S

V

V

active site: D218 (= D194), K222 (= K198)
binding nicotinamide-adenine-dinucleotide: I10 (= I7), A12 (≠ V9), G13 (= G10), V14 (≠ T11), V15 (= V12), E39 (≠ G32), A40 (≠ D33), T92 (vs. gap), S93 (vs. gap), P114 (≠ A98)

Query Sequence

>WP_013461155.1 NCBI__GCF_000183725.1:WP_013461155.1
MTRVGVIGVGTVGRAVVQILEENKSIITARSGDEISVKSGVVRDLSKVSDLSIAVSQDPY
DIVDDPEIDIVVELMGGVELPLAVVKKALQNGKAVVTANKALLAYHRYELQEIAGDIPFE
FEASVAGGIPIINALRDGLSANHILSIMGIMNGTCNFMLTKMMNEGTPFAEVLAEAQALG
YAEADPTFDIGGFDAAHKLLILASIAYGIDAKPEEILIEGIEGITPADIAFAKEFGYTIK
LLGIAKRDDAEVELRVHAALVKEDAMIAKIDGVMNGISVVGDRVGETLYYGPGAGGNATA
SAVVANIIDIVRSGKRSPMLGFNHPLEEGLRLKNSEDIRSKYYLRLRVSDKPGILAKITS
LFADESISIEAVIQRPSETECAHLLFATHEATERALHGLMAKLETLDAVLESPFMIRIV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory